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1.
Data Brief ; 28: 104975, 2020 Feb.
Artigo em Inglês | MEDLINE | ID: mdl-32226798

RESUMO

Oil palm (Elaeis guineensis Jacq.) from being almost unknown crop a mere three decades ago is now the most consumed and the most traded edible oil in the world. It is a highest yielding crop producing on an average 4 to 6 tons of oil per ha per year. Due to its innumerable uses in the food, oleochemicals and biofuel industries, cultivation of oil palm has expanded enormously in recent years. Since oil palm is a perennial monocotyledonous species with a single growing apex, the plant cannot be multiplied vegetatively and the conventional propagation through seed is limited by dormancy. Thus in vitro germination has become the key method for multiplication of elite oil palm genotypes. Although there are several reports on in vitro germination of oil palm, still there is a lack of an efficient & repeatable method. Hence an attempt is made to standardize the suitable culture media for direct germination from mature oil palm zygotic embryos. The data presented here represents the effect of genotypes, pretreatments and culture media on Mean Germination Time, Speed of Germination Index, Shoot Formation Index and Root Formation Index during in vitro culturing of oil palm zygotic embryos.

2.
Database (Oxford) ; 20202020 01 01.
Artigo em Inglês | MEDLINE | ID: mdl-32283556

RESUMO

Improvement of edible bananas (a triploid and sterile crop) through conventional breeding is a challenging task owing to its recalcitrant nature for seed set, prolonged crop duration. In addition, the need of huge man power at different stages of progeny development and evaluation often leads to mislabeling, poor data management and loss of vital data. All this can be overcome by the application of advanced information technology source. This ensured secure and efficient data management such as storage, retrieval and data analysis and further could assist in tracking the breeding status in real time. Thus, a user-friendly web-based banana breeding tracker (BBT) has been developed using MySQL database with Hypertext Preprocessor (PHP). This BBT works on all operating systems with access to multiple users from anywhere at any time. Quick responsive (QR) code labels can be generated by the tracker, which can be decoded using QR scanner. Also for each and every updated progress in breeding stages, a new QR code can be generated, which in turn reduce labeling errors. Moreover, the tracker has additional tools to search, sort and filter the data from the data sets for efficient retrieval and analysis. This tracker is being upgraded with phenotypic and genotypic data that will be made available in the public domain for hastening the banana improvement program.


Assuntos
Bases de Dados Factuais , Musa/crescimento & desenvolvimento , Musa/genética , Melhoramento Vegetal/métodos , Humanos , Armazenamento e Recuperação da Informação/métodos , Internet , Ploidias , Interface Usuário-Computador
3.
Water Environ Res ; 90(2): 101-109, 2018 Feb 01.
Artigo em Inglês | MEDLINE | ID: mdl-28600862

RESUMO

This paper presents the biophysical impact of various interventions made under watershed development programs, in terms of the creation of additional water resources, and resultant changes in land use and cropping patterns in the Bundelkhand region of Madhya Pradesh State, India. Both primary and secondary data gathered from randomly selected watersheds and their corresponding control villages were used in this study. Analysis revealed that emphasis was given primarily to the creation of water resources potential during implementation of the programs, which led to augmentation of surface and groundwater availability for both irrigation and non-agricultural purposes. In addition, other land based interventions for soil and moisture conservation, plantation activities, and so forth, were taken up on both arable and nonarable land, which helped to improve land slope and land use, cropping pattern, agricultural productivity, and vegetation cover.


Assuntos
Agricultura/métodos , Conservação dos Recursos Naturais/métodos , Recursos Hídricos/provisão & distribuição , Abastecimento de Água/métodos , Índia , Solo , Abastecimento de Água/estatística & dados numéricos
4.
PLoS One ; 12(2): e0171933, 2017.
Artigo em Inglês | MEDLINE | ID: mdl-28192462

RESUMO

The oil palm fruit forms (dura, pisifera and tenera) governed by the shell thickness gene (Sh) plays a major role in identification of fruit type and also influences palm oil yield. Identification of desired fruit type is a major asset to the breeders and oil palm workers for applications in breeding, seed certification and to reduce time, space and money spent on identification of fruit form. In the present study, we developed Sh gene specific primer pairs and bulk segregant analysis was done using 300 genomic and 8 genic SSR markers. We identified one cleaved amplified polymorphic site (CAPS) marker for differentiation of oil palm fruit type which produced two alleles (280 and 250bp) in dura genotypes, three alleles in tenera genotypes (550, 280, and 250bp) and one allele in pisifera genotypes (550bp). The shell allele sequencing results showed that two SNPs were present, of which SNP2 contributed for variation of fruit forms. The nucleotide 'A' was present in only dura genotypes, where as 'T' was present only in pisifera genotypes, which in turn led to the change of amino acid lysine to aspargine. The identified CAPS marker was validated on 300 dura, 25 pisifera and 80 tenera genotypes, 80 dura/ pisifera cross progenies and 60 lines of tenera/ tenera cross progeny. Association mapping of marker data with phenotypic data of eight oil yield related traits resulted in identification of seven significant QTLs by GLM approach, four by MLM approach at a significant threshold (P) level of 0.001. Significant QTLs were identified for fruit to bunch and oil to bunch traits, which explained R2 of 12.9% and 11.5% respectively. The CAPS marker used in the present study facilitate selection and timely distribution of desirable high yielding tenera sprouts to the farmers instead of waiting for 4-5 years. This saves a lot of land, time and money which will be a major breakthrough to the oil palm community.


Assuntos
Arecaceae/genética , Frutas/genética , Repetições de Microssatélites/genética , Polimorfismo de Nucleotídeo Único , Alelos , Sequência de Aminoácidos , Arecaceae/crescimento & desenvolvimento , Sequência de Bases , Mapeamento Cromossômico , Cromossomos de Plantas/genética , DNA de Plantas/química , DNA de Plantas/genética , Frutas/crescimento & desenvolvimento , Genes de Plantas/genética , Genótipo , Fenótipo , Melhoramento Vegetal/métodos , Locos de Características Quantitativas/genética , Reprodutibilidade dos Testes , Sementes/genética , Sementes/crescimento & desenvolvimento , Análise de Sequência de DNA , Homologia de Sequência de Aminoácidos , Homologia de Sequência do Ácido Nucleico
5.
Mol Genet Genomics ; 290(4): 1393-402, 2015 Aug.
Artigo em Inglês | MEDLINE | ID: mdl-25675870

RESUMO

This genetic diversity study aimed to estimate the population structure and explore the use of association mapping strategies to identify linked markers for bacterial resistance, growth and fruit quality in pomegranate collections from India. In total, 88 accessions including 37 cultivated types were investigated. A total of 112 alleles were amplified by use of 44 publicly available microsatellites for estimating molecular genetic diversity and population structure. Neighbor-joining analysis, model-based population structure and principal component analysis corroborated the genetic relationships among wild-type and cultivated pomegranate collections from India. Our study placed all 88 germplasm into four clusters. We identified a cultivated clade of pomegranates in close proximity to Daru types of wild-type pomegranates that grow naturally near the foothills of the Himalayas. Admixture analysis sorted various lineages of cultivated pomegranates to their respective ancestral forms. We identified four linked markers for fruit weight, titratable acidity and bacterial blight severity. PGCT001 was found associated with both fruit weight and bacterial blight, and the association with fruit weight during both seasons analyzed was significant after Bonferroni correction. This research demonstrates effectiveness of microsatellites to resolve population structure among the wild and cultivar collection of pomegranates and future use for association mapping studies.


Assuntos
Variação Genética , Lythraceae/genética , Repetições de Microssatélites/genética , Doenças das Plantas/genética , Análise de Variância , Mapeamento Cromossômico , Análise por Conglomerados , Resistência à Doença/genética , Fluxo Gênico , Genética Populacional , Interações Hospedeiro-Patógeno , Índia , Lythraceae/classificação , Lythraceae/microbiologia , Filogenia , Doenças das Plantas/microbiologia , Análise de Componente Principal , Estações do Ano , Especificidade da Espécie , Xanthomonas axonopodis/fisiologia
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