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1.
Braz. j. biol ; 83: e246934, 2023. tab, graf
Artigo em Inglês | LILACS-Express | MEDLINE, LILACSEXPRESS | ID: biblio-1285605

RESUMO

Abstract Physids belong to Class Gastropoda; Phylum Mollusca have important position in food web and act as bio indicators, pests and intermediate host. Being resistant these are called cockroaches of malacology. Physid snails were collected from different water bodies of Faisalabad (Punjab) and were identified up to species using morphological markers. The morphometry of the specimens was carried out with the help of a digital Vernier caliper in millimeters (mm) using linear measurement of shell characters. Linear regression analysis of the AL/SW ratio vs AL and SL/SW ratio vs AL indicated that allometric growth exists only in Physa acuta when compared with P.gyrina and P. fontinalis. This study will lead to assess the status of the Physid species in Central Punjab. The Principal component analysis shows that the Component 1 (Shell Length) and component 2 (Shell Width) are the most prolific components and nearly 80 percent of the identification. The distance between P. acuta and P. fontinalis is 5.4699, P. acuta and P. gyrina is 7.6411, P. fontinalis and P. gyrina is 16.6080 showing that P. acuta resembles with P. fontinalis, and both these specimens donot resemble with P. gyrina. P.acuta is an invasive species and shows bioactivity making it a potent candidate for bioactive substances.


Resumo Os físidos pertencem à classe Gastropoda; o filo Mollusca possui importante posição na teia alimentar e atua como bioindicador, praga e hospedeiro intermediário. Por serem resistentes, são chamadas baratas de malacologia. Os caramujos físidos foram coletados em diferentes corpos d'água de Faisalabad (Punjab) e identificados até as espécies por meio de marcadores morfológicos. A morfometria dos corpos de prova foi realizada com auxílio de paquímetro digital Vernier em milímetros (mm) por meio de medida linear dos caracteres da casca. A análise de regressão linear da razão AL / SW vs. AL e razão SL / SW vs. AL indicou que o crescimento alométrico existe apenas em Physa acuta quando comparado com P. gyrina e P. fontinalis. Este estudo levará a avaliar a situação das espécies de físido no Punjab Central. A análise do componente principal mostra que o componente 1 (comprimento da casca) e o componente 2 (largura da casca) são os componentes mais prolíficos e quase 80% da identificação. A distância entre P. acuta e P. fontinalis é 5,4699, P. acuta e P. gyrina é 7,6411, P. fontinalis e P. gyrina é 16,6080, mostrando que P. acuta se assemelha a P. fontinalis, e ambos os espécimes não se parecem com P. gyrina. P. acuta é uma espécie invasora e apresenta bioatividade, tornando-se uma candidata potente para substâncias bioativas.

2.
Braz. j. biol ; 83: e246984, 2023. tab, graf
Artigo em Inglês | LILACS-Express | MEDLINE, LILACSEXPRESS | ID: biblio-1285632

RESUMO

Abstract Physids belong to Class Gastropoda; belong to Phylum Mollusca and being bioindicators, intermediate hosts of parasites and pests hold a key position in the ecosystem. There are three species of Genus Physa i.e. P. fontinalis, Physa acuta and P. gyrina water bodies of Central Punjab and were characterized on the basis of molecular markers High level of genetic diversity was revealed by polymorphic RAPD, however SSR markers were not amplified. The multivariate analysis revealed polymorphism ranging from 9.09 percent to 50 percent among the three Physid species. Total number of 79 loci were observed for the three species under study and 24 loci were observed to be polymorphic. These RAPD fragment(s) can be developed into co dominant markers (SCAR) by cloning and can be further sequenced for the development of the Physa species specific markers to identify the introduced and native species in Pakistan.


Resumo Os físidos pertencem à classe Gastropoda; pertencem ao filo Mollusca e, sendo bioindicadores, hospedeiros intermediários de parasitas e pragas, ocupam uma posição-chave no ecossistema. Existem três espécies do gênero Physa, ou seja, P. fontinalis, Physa acuta e P. gyrina em corpos d'água do Punjab Central e foram caracterizadas com base em marcadores moleculares. Alto nível de diversidade genética foi revelado por RAPD polimórfico, no entanto os marcadores SSR não foram amplificados. A análise multivariada revelou polimorfismo variando de 9,09% a 50% entre as três espécies de Physid. Um número total de 79 loci foi observado para as três espécies em estudo e 24 loci foram observados como polimórficos. Esses fragmentos RAPD podem ser desenvolvidos em marcadores codominantes (SCAR) por clonagem e podem ser posteriormente sequenciados para o desenvolvimento de marcadores específicos da espécie Physa para identificar as espécies introduzidas e nativas no Paquistão.

3.
Clin Transl Oncol ; 2021 Oct 05.
Artigo em Inglês | MEDLINE | ID: mdl-34609675

RESUMO

Natural products, especially polyphenols (phenolic acids, lignans, and stilbenes) are suggested to be more potent anticancer drugs because of their no or less adverse effects, excess availability, high accuracy, and secure mode of action. In the present review, potential anticancer mechanisms of action of some polyphenols including phenolic acids, lignans, and stilbenes are discussed based on clinical, epidemiological, in vivo, and in vitro studies. The emerging evidence revealed that phenolic acids, lignans, and stilbenes induced apoptosis in the treatment of breast (MCF-7), colon (Caco-2), lung (SKLU-1), prostate (DU-145 and LNCaP), hepatocellular (hepG-2), and cervical (A-431) cancer cells, cell cycle arrest (S/G2/M/G1-phases) in gastric (MKN-45 and MKN-74), colorectal (HCT-116), bladder (T-24 and 5637), oral (H-400), leukemic (HL-60 and MOLT-4) and colon (Caco-2) cancer cells, and inhibit cell proliferation against the prostate (PC-3), liver (LI-90), breast (T47D and MDA-MB-231), colon (HT-29 and Caco-2), cervical (HTB-35), and MIC-1 cancer cells through caspase-3, MAPK, AMPK, Akt, NF-κB, Wnt, CD95, and SIRT1 pathways. Based on accumulated data, we suggested that polyphenols could be considered as a viable therapeutic option in the treatment of cancer cells in the near future.

4.
Braz J Biol ; 83: e246934, 2021.
Artigo em Inglês | MEDLINE | ID: mdl-34431912

RESUMO

Physids belong to Class Gastropoda; Phylum Mollusca have important position in food web and act as bio indicators, pests and intermediate host. Being resistant these are called cockroaches of malacology. Physid snails were collected from different water bodies of Faisalabad (Punjab) and were identified up to species using morphological markers. The morphometry of the specimens was carried out with the help of a digital Vernier caliper in millimeters (mm) using linear measurement of shell characters. Linear regression analysis of the AL/SW ratio vs AL and SL/SW ratio vs AL indicated that allometric growth exists only in Physa acuta when compared with P.gyrina and P. fontinalis. This study will lead to assess the status of the Physid species in Central Punjab. The Principal component analysis shows that the Component 1 (Shell Length) and component 2 (Shell Width) are the most prolific components and nearly 80 percent of the identification. The distance between P. acuta and P. fontinalis is 5.4699, P. acuta and P. gyrina is 7.6411, P. fontinalis and P. gyrina is 16.6080 showing that P. acuta resembles with P. fontinalis, and both these specimens donot resemble with P. gyrina. P.acuta is an invasive species and shows bioactivity making it a potent candidate for bioactive substances.


Assuntos
Espécies Introduzidas , Caramujos , Animais
5.
Braz J Biol ; 83: e246984, 2021.
Artigo em Inglês | MEDLINE | ID: mdl-34431913

RESUMO

Physids belong to Class Gastropoda; belong to Phylum Mollusca and being bioindicators, intermediate hosts of parasites and pests hold a key position in the ecosystem. There are three species of Genus Physa i.e. P. fontinalis, Physa acuta and P. gyrina water bodies of Central Punjab and were characterized on the basis of molecular markers High level of genetic diversity was revealed by polymorphic RAPD, however SSR markers were not amplified. The multivariate analysis revealed polymorphism ranging from 9.09 percent to 50 percent among the three Physid species. Total number of 79 loci were observed for the three species under study and 24 loci were observed to be polymorphic. These RAPD fragment(s) can be developed into co dominant markers (SCAR) by cloning and can be further sequenced for the development of the Physa species specific markers to identify the introduced and native species in Pakistan.


Assuntos
Gastrópodes , Espécies Introduzidas , Animais , Ecossistema , Paquistão , Filogenia , Técnica de Amplificação ao Acaso de DNA Polimórfico
7.
Pediatr Neurosurg ; 48(1): 48-50, 2012.
Artigo em Inglês | MEDLINE | ID: mdl-22831960

RESUMO

Encephaloceles arise from developmental defects in neural tube formation. These lesions contain brain and meninges which herniate through a defect in the skull. These may present as isolated malformations or rarely be associated with brain tumors. We hereby discuss a case of an unusual association of an occipital encephalocele with papillary intralymphatic angioendothelioma or Dabska tumor arising from the sac itself. The patient underwent resection of the herniated brain tissue with repair and closure of the dural defect. Histopathological examination revealed evidence of Dabska tumor from the sac. This is the first case report of the association of an occipital encephalocele with a rare vascular tumor, i.e. papillary intralymphatic angioendothelioma.


Assuntos
Encefalocele/cirurgia , Meningioma/cirurgia , Meningocele/cirurgia , Encefalocele/diagnóstico , Encefalocele/patologia , Feminino , Humanos , Recém-Nascido , Meningioma/diagnóstico , Meningioma/patologia , Meningocele/diagnóstico , Meningocele/patologia
8.
Pediatr Neurosurg ; 47(1): 22-30, 2011.
Artigo em Inglês | MEDLINE | ID: mdl-21613777

RESUMO

We present our experience after analyzing retrospectively the clinicopathological characteristics, surgical approaches, immediate and long-term postoperative complications and survival outcome of pediatric spinal epidural tumors treated over a period of 10 years from 2000 to 2009 in a tertiary-care hospital. Our study included benign and malignant tumors. The majority of our patients were boys (14:8). The duration of symptoms was longer in benign lesions. Non-Hodgkin's lymphoma was the commonest malignant tumor and aneurysmal bone cyst was the commonest benign tumor. The mean duration of follow-up was 19 months (range 3-48 months). Most of the patients improved in their neurological grade after surgery; however, the degree of excision had no bearing on the length of survival for malignant lesions.


Assuntos
Neoplasias Epidurais/diagnóstico , Neoplasias Epidurais/etnologia , Adolescente , Fatores Etários , Criança , Pré-Escolar , Neoplasias Epidurais/terapia , Feminino , Seguimentos , Humanos , Índia/etnologia , Estimativa de Kaplan-Meier , Masculino , Paquistão/etnologia , Estudos Retrospectivos , Neoplasias da Coluna Vertebral/diagnóstico , Neoplasias da Coluna Vertebral/etnologia , Neoplasias da Coluna Vertebral/terapia
9.
J Physiol ; 589(Pt 5): 1081-93, 2011 Mar 01.
Artigo em Inglês | MEDLINE | ID: mdl-21224236

RESUMO

Glucagon like peptide 1 (GLP-1) based therapies are now widely used for the treatment of type 2 diabetes. Developing our understanding of intestinal GLP-1 release may facilitate the development of new therapeutics aimed at targeting the GLP-1 producing L-cells. This study was undertaken to characterise the electrical activity of primary L-cells and the importance of voltage gated sodium and calcium channels for GLP-1 secretion. Primary murine L-cells were identified and purified using transgenic mice expressing a fluorescent protein driven by the proglucagon promoter. Fluorescent L-cells were identified within primary colonic cultures for patch clamp recordings. GLP-1 secretion was measured from primary colonic cultures. L-cells purified by flow cytometry were used to measure gene expression by microarray and quantitative RT-PCR. Electrical activity in L-cells was due to large voltage gated sodium currents, inhibition of which by tetrodotoxin reduced both basal and glutamine-stimulated GLP-1 secretion. Voltage gated calcium channels were predominantly of the L-type, Q-type and T-type, by expression analysis, consistent with the finding that GLP-1 release was blocked both by nifedipine and ω-conotoxin MVIIC. We observed large voltage-dependent potassium currents, but only a small chromanol sensitive current that might be attributable to KCNQ1. GLP-1 release from primary L-cells is linked to electrical activity and activation of L-type and Q-type calcium currents. The concept of an electrically excitable L-cell provides a basis for understanding how GLP-1 release may be modulated by nutrient, hormonal and pharmaceutical stimuli.


Assuntos
Estimulação Elétrica , Células Enteroendócrinas/metabolismo , Peptídeo 1 Semelhante ao Glucagon/metabolismo , Potenciais de Ação/fisiologia , Animais , Cálcio/metabolismo , Bloqueadores dos Canais de Cálcio/farmacologia , Canais de Cálcio/genética , Canais de Cálcio/metabolismo , Células Cultivadas , Eletrofisiologia , Células Enteroendócrinas/efeitos dos fármacos , Ensaio de Imunoadsorção Enzimática , Citometria de Fluxo , Ativação do Canal Iônico/efeitos dos fármacos , Ativação do Canal Iônico/fisiologia , Camundongos , Camundongos Transgênicos , Nifedipino/farmacologia , Análise de Sequência com Séries de Oligonucleotídeos , Reação em Cadeia da Polimerase Via Transcriptase Reversa
10.
J Clin Neurosci ; 18(3): 411-3, 2011 Mar.
Artigo em Inglês | MEDLINE | ID: mdl-21237658

RESUMO

Intraparenchymal schwannomas are rare. The usual presentation is of a classical intracranial mass lesion. We report a frontal intraparenchymal schwannoma in a pediatric patient with a history of seizure. An MRI scan revealed a uniformly enhancing tumor with perilesional edema. This type of tumor is rarely found in this location. It is important to recognize and distinguish a schwannoma from other more common frontal brain tumors as the outcome is good and recurrence is rare.


Assuntos
Neoplasias Encefálicas/patologia , Lobo Frontal/patologia , Neurilemoma/patologia , Adolescente , Neoplasias Encefálicas/complicações , Neoplasias Encefálicas/cirurgia , Lobo Frontal/cirurgia , Humanos , Imageamento por Ressonância Magnética , Masculino , Neurilemoma/complicações , Neurilemoma/cirurgia , Convulsões/etiologia , Convulsões/cirurgia
12.
Int J Soc Psychiatry ; 56(4): 326-35, 2010 Jul.
Artigo em Inglês | MEDLINE | ID: mdl-20472661

RESUMO

A diagnostic system for ICD-11 is proposed which commences with broad reorganization and simplification of the current categories and the use of clinically relevant specifiers. Such changes have implications for the positioning of diagnostic groups and lead to a range of possibilities for improving terminology and the juxtaposition of individual conditions. The development of ICD-11 provides the fi rst opportunity in almost two decades to improve the validity and reliability of the international classification system. Widespread change in broad categories and criteria cannot be justified by research that has emerged since the last revision. It would also be disruptive to clinical practice and might devalue past research work. However, the case for reorganization of the categories is stronger and has recently been made by an eminent international group of researchers (Andrews et al., 2009). A simpler, interlinked diagnostic system is proposed here which is likely to have fewer categories than its predecessor. There are major advantages of such a system for clinical practice and research and it could also produce much needed simplification for primary care (Gask et al., 2008) and the developing world (Wig, 1990; Kohn et al., 2004).


Assuntos
Classificação Internacional de Doenças/tendências , Transtornos Mentais/classificação , Transtornos Mentais/diagnóstico , Adulto , Pesquisa Biomédica/tendências , Criança , Previsões , Humanos , Internacionalidade , Encaminhamento e Consulta , Reprodutibilidade dos Testes , Reino Unido
14.
J Pediatr Neurosci ; 4(2): 127-30, 2009 Jul.
Artigo em Inglês | MEDLINE | ID: mdl-21887197

RESUMO

Congenital midline paraspinal cutaneous markers have been practically linked to the location and nature of neural-tissue lesions. One of the most interesting congenital midline paraspinal cutaneous markers has been the human tail in the lumbosacral region, with underlying spinal dysraphism. Human tails have many shapes and sizes and are usually localized to the lumbosacral region. After a complete neurological examination, the MRI is the most sensitive diagnostic modality to reveal the underlying occult spinal dysraphic state. Surgical excision is aimed at untethering of the spinal cord in symptomatic children and for aesthetic reasons in asymptomatic patients. Here we report an asymptomatic male child with normal external genitilia, whose tail is attached to the gluteal region like an adult phallus and investigations revealed an underlying spinal dysraphic state.

15.
Pediatr Neurosurg ; 44(3): 204-7, 2008.
Artigo em Inglês | MEDLINE | ID: mdl-18334844

RESUMO

OBJECTIVES: The aim of the study was the assessment of head injury caused by cricket ball injury in children. In underdeveloped countries, this is particularly important due to the absence of safety precautions. The study was undertaken to increase the public awareness of the need to reduce the morbidity related to this sport. METHODS: This was a prospective study in which all the children <18 years who had sustained head injury due to cricket balls were enrolled. RESULTS: The study included 27 children, 26 males and 1 female. Out of these 21 cases of head injury were due to hard plastic cricket balls and the rest were due to conventional cricket balls. In 25 patients, CT scan was done, which revealed a lesion in 21 of them. Six patients required surgery, 3 for extradural hematoma, 1 for acute subdural hematoma, 1 for contusion and 1 for compound depressed fracture. One death occurred in our series. There was no significant difference in the nature of injury sustained by either plastic or conventional cricket balls. CONCLUSION: Head injury due to cricket balls can lead to serious consequences even if an innocuous plastic ball is used in place of a conventional cricket ball. Public awareness apart from safety measures is required so that helmets are worn during playing. Change in the nature of the balls may bring a reduction in the severity of head injury.


Assuntos
Traumatismos Craniocerebrais/diagnóstico , Traumatismos Craniocerebrais/epidemiologia , Países em Desenvolvimento , Equipamentos Esportivos/efeitos adversos , Esportes/tendências , Criança , Traumatismos Craniocerebrais/terapia , Serviços Médicos de Emergência/métodos , Serviços Médicos de Emergência/tendências , Dispositivos de Proteção da Cabeça/tendências , Humanos , Estudos Prospectivos
16.
Iran J Immunol ; 4(1): 15-25, 2007 Mar.
Artigo em Inglês | MEDLINE | ID: mdl-17652839

RESUMO

BACKGROUND: Immunophenotypic characterization of the leukemic cells has been widely used as a tool for diagnosis, classification, stratification and prognosis of leukaemia. OBJECTIVE: To investigate the immunophenotypic subtype profiles of Iranian patients with acute lymphoblastic leukemia (ALL) and its association to disease outcome. METHODS: In this study, a total of 60 Iranian patients with ALL were immunophenotyped by flow cytometry using a panel of monoclonal antibodies specific for CD2, CD3, CD5, CD10, CD13, CD14, CD19, CD20, CD33, CD34, CD45, HLA-DR and TdT molecules. RESULTS: The samples were initially categorized into T-ALL (n=9), B-ALL (n=50) and mixed lineage (n=1) based on the expression patterns of CD3 and CD19 molecules. B-ALL patients could further be classified into four subtypes, including Pro-B (n=7, 11.7%), Pre-B I (n=28, 46.7%), Pre-B II (n=13, 21.7%) and immature/mature B cells (n=2, 3.3%) on the basis of expression of CD10, CD19, CD20, HLA-DR and TdT. Clinical manifestations and laboratory findings of the patients did not reveal association with immunophenotypic subtypes of ALL, with the exception of mediastinal mass and WBC count at the time of diagnosis which were found to be significantly higher in patients with T-ALL compared with B-ALL (p=0.001 and 0.014), respectively. CONCLUSION: Our results indicate that overall the immunophenotypic profile of Iranian ALL patients is similar to previous reports and it might be used for monitoring of minimal residual disease and prognosis.


Assuntos
Imunofenotipagem , Leucemia de Células B/imunologia , Leucemia-Linfoma Linfoblástico de Células Precursoras/imunologia , Adulto , Criança , Progressão da Doença , Humanos , Irã (Geográfico)/epidemiologia , Leucemia de Células B/diagnóstico , Leucemia de Células B/epidemiologia , Leucemia-Linfoma Linfoblástico de Células Precursoras/diagnóstico , Leucemia-Linfoma Linfoblástico de Células Precursoras/epidemiologia , Valor Preditivo dos Testes , Recidiva
17.
J Pak Med Assoc ; 53(4): 140-2, 2003 Apr.
Artigo em Inglês | MEDLINE | ID: mdl-12776897

RESUMO

OBJECTIVE: To determine the clinical presentation and find out the underlying cause of chronic renal failure (CRF). METHODS: This is a descriptive prospective study of patients attending children hospital, Chandka Medical College Larkana and National Institute of Child Health (Paediatric Nephrology Unit) Karachi from January 1993 to December 1996. RESULTS: Seventy eight confirmed cases of chronic renal failure (CRF) were included in the study. Out of them 51 were male and 27 females (M:F ratio 1.8:1). Most of the children (90%) presented after 3 years of age. Common clinical presentation were anaemia (93.5%) cases, growth retardation (74.3% cases), urinary complaints (65.3%) cases and hypertension (53.8%) cases. Common causes leading to CRF were reflux nephropathy (24.5%), posterior uretheral valves (23%), chronic glomerulonephritis (15.4%) and urolithiasis (14%). CONCLUSION: Early diagnosis and management of most common causes of CRF may prevent and delay the progression to end stage renal disease (ESRD).


Assuntos
Falência Renal Crônica/diagnóstico , Falência Renal Crônica/epidemiologia , Transplante de Rim/estatística & dados numéricos , Diálise Renal/estatística & dados numéricos , Distribuição por Idade , Criança , Pré-Escolar , Estudos de Coortes , Países em Desenvolvimento , Feminino , Humanos , Falência Renal Crônica/terapia , Masculino , Paquistão/epidemiologia , Prevalência , Prognóstico , Estudos Prospectivos , Fatores de Risco , Índice de Gravidade de Doença , Distribuição por Sexo , Fatores Socioeconômicos , Análise de Sobrevida
18.
Neurol India ; 50(2): 217-9, 2002 Jun.
Artigo em Inglês | MEDLINE | ID: mdl-12134196

RESUMO

The occurrence of bilateral extradural hematomas is an uncommon consequence of craniocerebral trauma and its incidence is variable in various studies ranging from 2-25%.1 We studied all cases of head injury brought to our institute over a period of 6 months and found the incidence of bilateral extradural hematomas to be 13.3%.


Assuntos
Traumatismos Craniocerebrais/complicações , Hematoma Epidural Craniano/epidemiologia , Hematoma Epidural Craniano/etiologia , Adulto , Criança , Hematoma Epidural Craniano/diagnóstico por imagem , Humanos , Incidência , Índia , Estudos Retrospectivos , Tomografia Computadorizada por Raios X
20.
Australas Radiol ; 40(4): 434-6, 1996 Nov.
Artigo em Inglês | MEDLINE | ID: mdl-8996907

RESUMO

Intercranial epidermoid tumours are benign lesions of congenital origin that are potentially curable. Although computerized axial tomography (CT) and magnetic resonance imaging (MRI) are usually diagnostic, a few atypical features sometimes make pre-operative diagnosis difficult. Two cases of cystic epidermoids with peripheral ring enhancement are reported. Relevant literature has been reviewed.


Assuntos
Encefalopatias/diagnóstico , Cisto Epidérmico/diagnóstico , Adulto , Encefalopatias/epidemiologia , Encefalopatias/cirurgia , Cisto Epidérmico/epidemiologia , Cisto Epidérmico/cirurgia , Feminino , Humanos , Imageamento por Ressonância Magnética , Masculino , Tomografia Computadorizada por Raios X
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