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1.
Oncogene ; 28(5): 773-80, 2009 Feb 05.
Artigo em Inglês | MEDLINE | ID: mdl-18997822

RESUMO

The receptor for macrophage colony-stimulating factor 1 receptor (CSF1R) is a product of the proto-oncogene c-fms and a member of the class III transmembrane tyrosine kinase receptor family. Earlier, we described increased mRNA expression of CSF1R in human telomerase reverse transcriptase (hTERT) immortalized human ovarian surface epithelial (IOSE) cell lines derived from a single donor. Here, we further describe that CSF1R is upregulated at both the mRNA and protein level in hTERT immortalized human normal OSE cells from two different donors and in hTERT immortalized human pancreatic ductal epithelial cells. CSF1R was not upregulated in hTERT immortalized epithelial clones that subsequently underwent senescence or in immortalized fibroblasts. Upon stimulation by the CSF1R ligand CSF1, the immortalized epithelial cell lines showed rapid internalization of CSF1R with concomitant down-modulation and colocalization of phosphorylated NFkappaBp65 with hTERT protein, hTERT translocation into the nucleus and the binding of c-Myc to the hTERT promoter region. Reducing the expression of CSF1R using short hairpin interfering RNA abolished these effects and also decreased cell survival and the number of population doublings under suboptimal culture conditions. The telomerase inhibitor GRN163L confirmed a role for telomerase in the cleavage of the intracellular domain of CSF1R. On the basis of these findings, we suggest that CSF1R may be a critical factor facilitating hTERT immortalization of epithelial cells.


Assuntos
Transformação Celular Neoplásica/genética , Células Epiteliais/patologia , Receptor de Fator Estimulador de Colônias de Macrófagos/fisiologia , Telomerase/genética , Linhagem Celular Transformada , Células Epiteliais/metabolismo , Regulação Neoplásica da Expressão Gênica/efeitos dos fármacos , Humanos , Fator Estimulador de Colônias de Macrófagos/farmacologia , Receptor de Fator Estimulador de Colônias de Macrófagos/genética , Receptor de Fator Estimulador de Colônias de Macrófagos/metabolismo , Telomerase/metabolismo , Transfecção , Regulação para Cima/efeitos dos fármacos
2.
Rev Elev Med Vet Pays Trop ; 49(2): 110-3, 1996.
Artigo em Inglês | MEDLINE | ID: mdl-9008959

RESUMO

An outbreak of infectious bursal disease (IBD) occurred concurrently with acute septicaemic colibacillosis in 15 week old prelayer hens. The septicaemia was preceded by a subclinical IBD. Mortality in the outbreak began with lesions of septicaemia and Escherichia coli was isolated from the heart blood of the birds. After antibiotic treatment of the bacteraemia, mortality continued, spiked, declined and then ceased. IBD was confirmed by bursal lesions characterized by severe lymphocytolysis and cystic degeneration of the lymphoid follicles. Out of 253 birds, 42 (16.60%) died within eight days. The circumstances of the outbreak suggested that lack of IBD booster vaccination favoured the establishment of subclinical IBD, which suppressed immunity to predispose the birds to colisepticaemia.


Assuntos
Bacteriemia/veterinária , Galinhas , Infecções por Coronavirus/veterinária , Infecções por Escherichia coli/veterinária , Vírus da Bronquite Infecciosa , Doenças das Aves Domésticas/epidemiologia , Animais , Bacteriemia/complicações , Bacteriemia/epidemiologia , Infecções por Coronavirus/complicações , Infecções por Coronavirus/epidemiologia , Infecções por Escherichia coli/complicações , Infecções por Escherichia coli/epidemiologia , Feminino , Nigéria/epidemiologia
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