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2.
Clin Exp Immunol ; 152(1): 102-10, 2008 Apr.
Artigo em Inglês | MEDLINE | ID: mdl-18241224

RESUMO

Ulcerative colitis (UC) is a multi-factorial inflammatory disease of the colon and rectum. The present study was undertaken to investigate the effect of taurine, an anti-oxidant amino acid, on oxidative stress and the expression of apoptosis-related proteins, pro-apoptotic Bax and anti-apoptotic B cell lymphoma-2 (Bcl-2) in colon tissue in rats with 2,4,6-trinitrobenzene sulphonic acid (TNBS)-induced colitis. Rats received taurine (1.5% w/v) in drinking water for 15 days before and 15 days after administration of TNBS solution. Then, colonic myeloperoxidase (MPO) activity, malondialdehyde (MDA) and glutathione (GSH) levels, and Bax and Bcl-2 expression were measured. TNBS-induced colitis caused significantly increased MPO activity and MDA levels and decreased GSH levels in colon tissue compared to controls. Increase in Bax expression and decrease in Bcl-2 expression were detected in colon of rats with TNBS-induced colitis. Taurine treatment was associated with amelioration in macroscopic and microscopic colitis scores, decreased colonic MPO activity and MDA levels and increased GSH levels in TNBS-induced colitis. In addition, taurine reduced the expression of Bax and prevented the loss of Bcl-2 proteins in colon tissue of rats with TNBS-induced colitis. The results of this study show that taurine administration may exert beneficial effects in UC by decreasing inflammatory reactions, oxidative stress and apoptosis.


Assuntos
Proteínas Reguladoras de Apoptose/metabolismo , Colite Ulcerativa/metabolismo , Estresse Oxidativo/efeitos dos fármacos , Taurina/farmacologia , Animais , Apoptose/efeitos dos fármacos , Western Blotting/métodos , Colite Ulcerativa/induzido quimicamente , Colite Ulcerativa/patologia , Colo , Modelos Animais de Doenças , Glutationa/metabolismo , Masculino , Malondialdeído/metabolismo , Peroxidase/metabolismo , Proteínas Proto-Oncogênicas c-bcl-2/metabolismo , Ratos , Ratos Wistar , Ácido Trinitrobenzenossulfônico , Proteína X Associada a bcl-2/metabolismo
3.
Neurol Sci ; 28(1): 31-4, 2007 Mar.
Artigo em Inglês | MEDLINE | ID: mdl-17385092

RESUMO

Alzheimer's disease (AD) is defined pathologically by the presence of beta-amyloid plaques, neurofibrillary tangles and extensive neuronal loss. Evidence indicates that increased DNA damage may contribute to neuronal loss in AD. Recently, it has been shown that in AD neurons have a reduced capacity for some types of DNA repair. Polymorphisms in DNA repair genes may be associated with differences in repair efficiency of DNA damage. Variants of several DNA repair genes, including the base excision repair gene XRCC1, have been described previously. We hypothesised that Arg194Trp polymorphism of XRCC1 gene may contribute to genetic susceptibility for AD. In order to test this hypothesis, we investigated Arg194Trp polymorphism at the XRCC1 gene in the DNA samples of 98 patients with AD and 95 healthy subjects. The frequency of the Trp allele was more pronounced among cases (11.2%) compared with controls (5.8%). On combining the homozygous and heterozygous variants of each codon, the variants seemed to be at twofold risk of AD, although the risk estimates were not statistically significant (OR=1.95, 95% CI 0.88-4.34, p=0.09). In addition, the 194Trp allele revealed a borderline significance (OR=2.05, 95% CI 0.96-4.37, p=0.056). According to our results, it may be speculated that the polymorphic variants of XRCC1 codon 194 have a role in the development of AD.


Assuntos
Doença de Alzheimer/genética , Arginina/genética , Proteínas de Ligação a DNA/genética , Polimorfismo Genético , Risco , Triptofano/genética , Idoso , Idoso de 80 Anos ou mais , Doença de Alzheimer/epidemiologia , Estudos de Casos e Controles , Feminino , Frequência do Gene , Genótipo , Humanos , Masculino , Pessoa de Meia-Idade , Proteína 1 Complementadora Cruzada de Reparo de Raio-X
4.
Clin Exp Med ; 7(4): 158-63, 2007 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-18188529

RESUMO

Oxidative modifications of apo-B-containing lipoproteins (LDL+VLDL) appear to play a role in atherogenesis. Increased atherosclerosis is one of the major causes of morbidity and mortality during ageing. The aim of this study was to investigate the susceptibility of serum and LDL+VLDL to lipid peroxidation in 12 young (6 months) and 12 old (22 months) rats. Serum endogenous malondialdehyde (MDA) and diene conjugate (DC) levels were found to be increased by 24.9% and 30.0% respectively, in old rats. In addition, 2,2'-azobis-(2-amidinopropane) hydrochloride (AAPH)-induced lipid peroxide levels were increased in serum of old rats. Although serum vitamin E levels were significantly increased (27.4%), there was a significant decrease in cholesterol-adjusted vitamin E levels (14.3%) in old rats. Serum vitamin C and total sulphydryl levels were found to be decreased by 25.5% and 22.7% respectively. The ferric reducing ability of plasma (FRAP) was also lower (15.9%) in old rats. Endogenous DC and copper-induced MDA levels were significantly higher (65.1% and 26.7% respectively) in LDL+VLDL fractions obtained from EDTA-plasma by dextrane sulphate and MgCl(2) solution in old rats. The propagation rate and maximal amount of DC increased, but the lag phase and t (max) were shortened in LDL+VLDL fractions of old rats. Our results clearly indicate that the susceptibility of whole serum and LDL+VLDL fractions to lipid peroxidation is increased in aged rats.


Assuntos
Envelhecimento/metabolismo , Peroxidação de Lipídeos , Lipoproteínas LDL/metabolismo , Lipoproteínas VLDL/metabolismo , Amidinas/farmacologia , Animais , Masculino , Malondialdeído/sangue , Ratos , Ratos Wistar
5.
Amino Acids ; 32(1): 115-9, 2007 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-16622601

RESUMO

We aimed to investigate the effect of decreased taurine levels on endogenous and induced lipid peroxide levels in liver, brain, heart and erythrocytes as well as prooxidant and antioxidant balance in the liver of rats administered beta-alanine (3%, w/v) in drinking water for 1 month to decrease taurine levels of tissues. This treatment caused significant decreases in taurine levels of liver (86%), brain (36%) and heart (15%). We found that endogenous and ascorbic acid-, NADPH- and cumene hydroperoxide-induced malondialdehyde (MDA) levels did not change in the liver, brain and heart homogenates following beta-alanine treatment. Also, H(2)O(2)-induced MDA levels remained unchanged in erythrocytes. In addition, we did not observe any changes in levels of MDA, diene conjugates, glutathione, alpha-tocopherol, ascorbic acid and the activities of superoxide dismutase, glutathione peroxidase and glutathione transferase in the liver. According to this, buffering or sequestering capacity of tissues to exogenous stimuli was not influenced by reduced taurine levels in tissues of rats.


Assuntos
Peroxidação de Lipídeos/efeitos dos fármacos , Taurina/metabolismo , beta-Alanina/farmacologia , Animais , Antioxidantes/metabolismo , Derivados de Benzeno/farmacologia , Peróxido de Hidrogênio/farmacologia , Masculino , Malondialdeído/metabolismo , NADP/metabolismo , Especificidade de Órgãos/efeitos dos fármacos , Oxidantes/farmacologia , Ratos , Ratos Wistar
6.
Clin Exp Med ; 3(4): 237-42, 2004 Apr.
Artigo em Inglês | MEDLINE | ID: mdl-15103515

RESUMO

In this study, we investigated serum pro-oxidantantioxidant balance in 210 healthy subjects divided into groups with low and high atherogenic risk according to the levels of serum total cholesterol (TC) and low-density lipoprotein-cholesterol (LDL-C) and high-density lipoprotein- cholesterol (HDL-C). Diene conjugate (DC), malondialdehyde (MDA), polyunsaturated fatty acid (PUFA), vitamin E, and vitamin C levels and antioxidant activity (AOA) were determined in the serum. Endogenous DC and copper-induced MDA levels were also measured in the LDL fraction isolated by precipitation with buffered heparin from plasma in 80 healthy subjects with different serum LDL-C levels. Subjects with a high atherogenic risk had significantly higher plasma DC, MDA, and PUFA levels, but lower vitamin E/TC values and AOA than subjects with low atherogenic risk. Endogenous DC and copper-induced MDA levels in the LDL fraction were increased in subjects with serum LDL-C levels higher than 4.14 mM compared with those with normal LDL-C levels. In conclusion, this study clearly indicates that a disturbance in serum pro-oxidant-antioxidant balance and an increase in LDL oxidation are concomitant with higher TC and LDL-C and lower HDL-C levels in the serum.


Assuntos
Antioxidantes/análise , Colesterol/sangue , Lipoproteínas LDL/sangue , Lipoproteínas LDL/metabolismo , Oxidantes/sangue , Adulto , Idoso , Antioxidantes/metabolismo , Interpretação Estatística de Dados , Feminino , Humanos , Peroxidação de Lipídeos , Masculino , Pessoa de Meia-Idade , Oxidantes/metabolismo , Oxirredução
7.
Fitoterapia ; 74(7-8): 618-23, 2003 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-14630164

RESUMO

Ecballium elaterium fruit juice is used for the treatment of sinusitis in Turkish folk medicine. The aim of this study was to increase the yield of cucurbitacin B, an anti-inflammatory compound previously isolated in various organs of E. elaterium, through tissue culture techniques. Higher yields of cucurbitacin B (1.126%) were obtained from the first subculture calluses from stem nodes in the presence of benzyl adenine (BA; 1 mg/l) and naphtalene acetic acid (NAA; 0.1 mg/l) in comparison with the yields obtained from plant material (0.01%).


Assuntos
Anti-Inflamatórios não Esteroides/metabolismo , Cucurbitaceae/crescimento & desenvolvimento , Fitoterapia , Triterpenos/metabolismo , Células Cultivadas , Cucurbitaceae/citologia , Meios de Cultura , Frutas , Humanos , Extratos Vegetais/química , Folhas de Planta/citologia , Folhas de Planta/crescimento & desenvolvimento , Caules de Planta/citologia , Caules de Planta/crescimento & desenvolvimento
8.
Clin Exp Med ; 2(4): 171-4, 2003 Feb.
Artigo em Inglês | MEDLINE | ID: mdl-12624707

RESUMO

Endogenous malondialdehyde and diene conjugate levels, the susceptibility of apolipoprotein B-containing lipoproteins to copper-induced lipid peroxidation, and antibody titer against oxidized low-density lipoproteins were increased, but serum antioxidant activity was unchanged in obese women. Serum cholesterol, low-density lipoproteincholesterol, and trigliceride levels were also elevated, but high-density lipoprotein-cholesterol levels remained unchanged in obese women. In vitro, oxidation of apolipoprotein B-containing lipoproteins and levels of antibody against oxidized low-density lipoprotein correlated with body mass index, serum total cholesterol, and low-density lipoproteincholesterol levels in obese women. These results indicate that obesity is associated with increases in endogenous lipid peroxides, oxidation of low-density lipoproteins, and lipids in serum.


Assuntos
Peróxidos Lipídicos/sangue , Lipoproteínas LDL/metabolismo , Obesidade/metabolismo , Adulto , Arteriosclerose/metabolismo , Índice de Massa Corporal , Colesterol/sangue , Feminino , Humanos , Malondialdeído/sangue , Pessoa de Meia-Idade , Oxirredução , Fatores de Risco , Estatística como Assunto
9.
Hum Exp Toxicol ; 21(7): 359-64, 2002 Jul.
Artigo em Inglês | MEDLINE | ID: mdl-12269697

RESUMO

Hepatic cirrhosis is produced in rats by administration of thioacetamide (TAA) (0.3 g/L tap water for a period of three months). This treatment caused an increase in oxidative stress in the liver. Lipopolysaccharide (LPS) administration (5 mg/kg) to rats with cirrhosis was observed to increase hepatotoxicity as well as oxidative stress according to biochemical and histopathological findings. However, aminoguanidine (AG), an inducible nitric oxide synthase (iNOS) inhibitor, plus N-acetylcysteine (NAC) treatment reduced the LPS-augmented hepatotoxicity in rats with cirrhosis without making any changes in oxidative stress in the liver.


Assuntos
Acetilcisteína/uso terapêutico , Guanidinas/uso terapêutico , Lipopolissacarídeos/toxicidade , Cirrose Hepática Experimental/tratamento farmacológico , Óxido Nítrico Sintase/antagonistas & inibidores , Análise de Variância , Animais , Sequestradores de Radicais Livres/uso terapêutico , Lipopolissacarídeos/antagonistas & inibidores , Cirrose Hepática Experimental/enzimologia , Cirrose Hepática Experimental/patologia , Masculino , Estresse Oxidativo/efeitos dos fármacos , Ratos , Ratos Wistar
10.
Int J Androl ; 25(3): 180-5, 2002 Jun.
Artigo em Inglês | MEDLINE | ID: mdl-12031047

RESUMO

A fourth Na,K-ATPase alpha isoform, which was found to be abundant in testes, was proved to be a catalytical subunit of the enzyme. Recently, it has been shown that the alpha 4 isoform along with alpha 1 is expressed in the midpiece of the flagellum of mature rat sperm and the inhibition of alpha 4 with ouabain led to sperm immotility. In this study, sperm from 135 males with normal semen profile and 50 males with oligoasthenospermia were treated with 10-5 and 10-2 M ouabain solutions to inhibit alpha 4, and alpha 4 plus alpha 1 isoforms, respectively. In males with normal semen profile, sperm motility has been demonstrated to decrease with time to almost the same level with both ouabain solutions. In oligoasthenospermic males motility was also found almost completely lost. These observations showed us that the alpha 4 isoform may be held responsible for human sperm motility. When sperm plasma membrane Na,K-ATPase activity was assayed for both normal and oligoasthenospermic males, a significant decrease in enzyme activity of males with oligoasthenospermia was observed (p < 0.05). In our recent study, sperm motility was found decreased by treatment with peroxynitrite (ONOO-). To investigate the effect of ONOO- on sperm Na,K-ATPase activity, sperm plasma membranes were treated with 100 microM ONOO- and plasma membrane Na,K-ATPase activity was observed to be significantly decreased (p < 0.05). Although total sulfhydryl (SH) content of sperm plasma membrane was also found significantly lower, no correlation was found between Na,K-ATPase activity and SH content.


Assuntos
ATPase Trocadora de Sódio-Potássio/fisiologia , Motilidade Espermática/fisiologia , Espermatozoides/enzimologia , Adulto , Humanos , Masculino , Ácido Peroxinitroso/farmacologia , Motilidade Espermática/efeitos dos fármacos , Espermatozoides/fisiologia
11.
J Cancer Res Clin Oncol ; 128(2): 91-5, 2002 Feb.
Artigo em Inglês | MEDLINE | ID: mdl-11862479

RESUMO

PURPOSE: In this study, 16 paired samples of colorectal and gastric cancers and adjacent non-neoplastic tissues were analysed for the determination of glutathione S-transferase (GST) activities and the expression of GST-pi. METHODS: Western blotting procedure as well as plasma GST-pi levels were used. RESULTS: GST activities were found to be increased in malignant tissues of patient compared with adjacent normal tissues. A significant correlation was detected between GST activity and GST-pi expression in malignant tissues of patients. Plasma GST-pi levels increased in patients compared to aged-matched control subjects. When the patients were grouped according to TNM stage, GST-pi expression in malignant tissues as well as plasma GST-pi levels were higher in patients with more advanced tumor stages. CONCLUSIONS: Our results indicate that GST-pi expression in malignant tissues and plasma GST-pi levels in human colorectal and gastric cancers increased depending on the stages of tumor.


Assuntos
Neoplasias Colorretais/enzimologia , Regulação Neoplásica da Expressão Gênica , Glutationa Transferase/biossíntese , Isoenzimas/biossíntese , Estadiamento de Neoplasias , Neoplasias Gástricas/enzimologia , Adulto , Idoso , Idoso de 80 Anos ou mais , Western Blotting , Neoplasias Colorretais/patologia , Eletroforese em Gel de Poliacrilamida , Feminino , Glutationa S-Transferase pi , Glutationa Transferase/metabolismo , Humanos , Isoenzimas/metabolismo , Masculino , Pessoa de Meia-Idade , Neoplasias Gástricas/patologia
12.
Hum Exp Toxicol ; 20(7): 373-6, 2001 Jul.
Artigo em Inglês | MEDLINE | ID: mdl-11530836

RESUMO

Glutaredoxin is an important enzyme in thiol homeostasis. As a thioltransferase, it reduces oxidized thiols. It also has dehydroascorbate reductase (DHAR) activity to reduce dehydroascorbate (DHA) to ascorbic acid. Peroxynitrite (ONOO-) is one of the most active elements of oxidative stress that can be formed wherever nitric oxide and superoxide are produced simultaneously. ONOO- is known to react with free thiols easily. To observe the effect of ONOO on glutaredoxin, rat liver cytosolic fractions were incubated with 0-250 microM ONOO-. Thioltransferase activity was found to be decreased as ONOO concentration increased. The inhibition was not reversible with dithiothreitol (DTT). In cytosol besides glutaredoxin, another enzyme with DHAR activity is also present. In our study, the cytosolic DHAR activity which consisted both enzymes, was also inhibited by ONOO-, but DTT was able to return the activity almost completely.


Assuntos
Ácido Peroxinitroso/farmacologia , Proteínas/metabolismo , Animais , Técnicas de Cultura de Células , Glutarredoxinas , Fígado/efeitos dos fármacos , Masculino , Oxirredutases/efeitos dos fármacos , Oxirredutases/metabolismo , Proteínas/efeitos dos fármacos , Ratos , Ratos Wistar
13.
J Pharm Biomed Anal ; 26(1): 111-21, 2001 Aug.
Artigo em Inglês | MEDLINE | ID: mdl-11451648

RESUMO

Lime flowers are used for various medicinal purposes in phytotherapy. Flavonoids, volatile oil and mucilage components are known as the active ingredients. In European Pharmacopoeia (EP), a simple thin-layer chromatographic (TLC) technique, which based on the analysis of the flavonoid composition was defined for the qualitative analysis of the drug. In this study, flavonoid composition in the flowers, bracts and leaves of the officinal species, Tilia platyphyllos were studied using a reversed-phase high performance liquid chromatographic (HPLC) technique, in order to develop a rapid, reliable and accurate method for quantitative analysis. The results were further compared with in those parts of two common species growing in Turkey, Tilia rubra and Tilia argentea. Results of the present study revealed that flavonoid composition of each lime species possesses a specific fingerprint HPLC chromatogram depending upon the parts used and evaluation of the data might be helpful in the quality assurance as well as determination of adulteration of the crude drug.


Assuntos
Cromatografia Líquida de Alta Pressão/métodos , Flavonoides/análise , Extratos Vegetais/análise , Plantas Medicinais/química , Garantia da Qualidade dos Cuidados de Saúde , Reprodutibilidade dos Testes
15.
Hum Exp Toxicol ; 20(5): 251-4, 2001 May.
Artigo em Inglês | MEDLINE | ID: mdl-11476157

RESUMO

Thioacetamide (TAA) administration (0.3 g/l of tap water for a period of 3 months) to rats resulted in hepatic cirrhosis as assessed by biochemical and histopathological findings. This treatment caused an increase in the levels of malondialdehyde (MDA) and diene conjugates (DCs) and a decrease in the levels of glutathione (GSH), vitamin E, vitamin C and the activities of glutathione peroxidase (GSH-Px) in the liver of rats. Superoxide dismutase (SOD) activities were unchanged. Taurine (2% w/w, added to the chow diet) was administered together with TAA (0.3 g/l of drinking water) for 3 months. Taurine was found to decrease TAA-induced hepatic lipid peroxidation and to increase TAA-depleted vitamin E levels and GSH-Px activities. Histopathological findings also suggested that taurine has an inhibitive effect on TAA-induced hepatic cirrhosis. These results indicate that taurine treatment has a protective effect against TAA-induced liver cirrhosis by decreasing oxidative stress.


Assuntos
Carcinógenos/efeitos adversos , Cirrose Hepática/induzido quimicamente , Cirrose Hepática/prevenção & controle , Estresse Oxidativo/efeitos dos fármacos , Taurina/farmacologia , Tioacetamida/efeitos adversos , Administração Oral , Animais , Glutationa/metabolismo , Fígado/enzimologia , Cirrose Hepática/veterinária , Masculino , Malondialdeído/metabolismo , Ratos , Ratos Wistar , Superóxido Dismutase/metabolismo
16.
Hum Exp Toxicol ; 20(1): 23-7, 2001 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-11339621

RESUMO

Thioacetamide (TAA) administration (three consecutive intraperitoneal injections of 400 mg/kg at 24-h interval) to rats resulted in hepatic injury as assessed by the measurement of serum transaminase activities and histopathological findings. This treatment caused an increase in the levels of malondialdehyde (MDA), diene conjugates (DCs) and glutathione (GSH) and the activity of superoxide dismutase SOD ), and a decrease in the levels of vitamins E and C and the activity of glutathione peroxidase (GSH-Px) in the liver of rats. Taurine administration (400 mg/kg, i.p., every 12 h and started 24 h prior to the first TAA injection) was found to decrease serum transaminase activities and hepatic lipid peroxidation without any significant change in hepatic antioxidant system. Histopathological findings also suggested that taurine has ameliorated effect on TAA-induced hepatic necrosis. These results indicate that taurine treatment, together with TAA administration, diminished the severity of the liver injury by decreasing oxidative stress due to its possible scavenger effect.


Assuntos
Doença Hepática Induzida por Substâncias e Drogas/prevenção & controle , Taurina/uso terapêutico , Tioacetamida/antagonistas & inibidores , Tioacetamida/toxicidade , Alanina Transaminase/sangue , Animais , Aspartato Aminotransferases/sangue , Doença Hepática Induzida por Substâncias e Drogas/patologia , Feminino , Radicais Livres/metabolismo , Fígado/patologia , Ratos , Ratos Wistar
17.
Res Commun Mol Pathol Pharmacol ; 109(5-6): 299-308, 2001.
Artigo em Inglês | MEDLINE | ID: mdl-12889513

RESUMO

Malondialdehyde (MDA) and diene conjugates (DC) and vitamin C levels and the activities of glutathione peroxidase (GSH-Px) and superoxide dismutase (SOD) were determined in the liver and kidney and their mitochondrial fractions of guinea pigs 48 h after the injection of L-buthionine-(S,R)-sulfoximine (BSO), a glutathione (GSH) depleting agent. In BSO-induced GSH depletion, lipid peroxidation and SOD activities were found to be increased but GSH-Px activities did not change in the liver and kidney and their mitochondrial fractions. In addition, vitamin C levels remained unchanged in the liver and kidney homogenates. These results indicate that GSH depletion may influence oxidative stress in the liver and kidney and their mitochondrial fractions of guinea pigs.


Assuntos
Butionina Sulfoximina/toxicidade , Inibidores Enzimáticos/toxicidade , Glutationa Peroxidase/metabolismo , Glutationa/deficiência , Rim/efeitos dos fármacos , Peroxidação de Lipídeos/efeitos dos fármacos , Mitocôndrias Hepáticas/efeitos dos fármacos , Animais , Cobaias , Rim/metabolismo , Rim/patologia , Masculino , Malondialdeído/metabolismo , Mitocôndrias Hepáticas/metabolismo , Mitocôndrias Hepáticas/patologia , Superóxido Dismutase/metabolismo
18.
Jpn J Cancer Res ; 91(12): 1258-63, 2000 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-11123424

RESUMO

Lipid peroxide levels and superoxide dismutase (SOD), glutathione peroxidase (GSH-Px), and glutathione transferase (GST) activities were investigated in mitochondrial fractions obtained from tumorous and nontumorous colorectal tissues of fourteen patients with colon and rectum cancer. Histopathological evaluations, including type, stage, necrosis and lymphocyte infiltration were also performed for each patient. The activities of SOD, GSH-Px and GST were increased significantly, but lipid peroxide levels remained unchanged in mitochondria obtained from tumors compared to adjacent normal tissues of subjects with colorectal cancer. When the patients were grouped according to their histopathological evaluation, such as type, stage, necrosis and lymphocyte infiltration, no relationship was observed between the histopathological results and the mitochondrial lipid peroxidation or antioxidant enzyme activities.


Assuntos
Adenocarcinoma/metabolismo , Neoplasias Colorretais/metabolismo , Glutationa Peroxidase/metabolismo , Glutationa Transferase/metabolismo , Peroxidação de Lipídeos , Mitocôndrias/metabolismo , Superóxido Dismutase/metabolismo , Adenocarcinoma/patologia , Adenocarcinoma/cirurgia , Colo/metabolismo , Neoplasias do Colo/metabolismo , Neoplasias do Colo/patologia , Neoplasias do Colo/cirurgia , Neoplasias Colorretais/patologia , Neoplasias Colorretais/cirurgia , Feminino , Humanos , Mucosa Intestinal/metabolismo , Linfócitos do Interstício Tumoral/patologia , Masculino , Pessoa de Meia-Idade , Necrose , Estadiamento de Neoplasias , Neoplasias Retais/metabolismo , Neoplasias Retais/patologia , Neoplasias Retais/cirurgia , Reto/metabolismo
19.
J Lab Clin Med ; 136(4): 287-91, 2000 Oct.
Artigo em Inglês | MEDLINE | ID: mdl-11039849

RESUMO

Increased oxidative stress has been speculated to be one possible mechanism of ethanol toxicity. This study evaluates malondialdehyde (MDA) and protein carbonyl content in serum as markers of oxidative stress and DNA damage in lymphocytes in the same patients with chronic alcoholism. Patients with chronic alcoholism showed a significant increase in MDA levels and protein carbonyl content of their serum as compared with non-alcoholic control subjects. Increases in endogenous and H2O2-induced DNA damage were also observed in lymphocytes of patients with chronic alcoholism. In addition, there were significant correlations between endogenous and H2O2-induced DNA damage and serum MDA or protein carbonyl content in patients with chronic alcoholism. These results clearly indicate the presence of oxidative stress in patients with chronic alcoholism.


Assuntos
Alcoolismo/genética , Alcoolismo/metabolismo , Dano ao DNA , Metabolismo dos Lipídeos , Albumina Sérica/metabolismo , Adulto , Alanina Transaminase/sangue , Aspartato Aminotransferases/sangue , Depressores do Sistema Nervoso Central/toxicidade , Doença Crônica , Etanol/toxicidade , Feminino , Humanos , Peróxido de Hidrogênio , Linfócitos/enzimologia , Masculino , Malondialdeído/sangue , Pessoa de Meia-Idade , Estresse Oxidativo/fisiologia
20.
Life Sci ; 66(20): 1905-13, 2000 Apr 07.
Artigo em Inglês | MEDLINE | ID: mdl-10821115

RESUMO

Cytotoxic effects of ionizing radiation on gastrointestinal epithelium may be related to oxidative stress. In this study, we wanted to investigate the effects of selenium, vitamin E and selenium plus vitamin E pretreatments prior to whole abdominal irradiation on intestinal injury. Irradiation caused increased lipid peroxide and decreased GSH levels in the intestine. Intestinal superoxide dismutase and glutathione peroxidase activities were increased, but glutathione transferase activity decreased following irradiation. Selenium and/or vitamin E pretreatments ameliorated these disturbances in prooxidant-antioxidant balance. This amelioriation has been verified with histopathological findings. These results indicate that antioxidant pretreatments prior to irradiation may have some beneficial effects against irradiation-induced intestinal injury.


Assuntos
Enterite/prevenção & controle , Íleo/efeitos dos fármacos , Íleo/efeitos da radiação , Lesões Experimentais por Radiação/prevenção & controle , Protetores contra Radiação/farmacologia , Selênio/farmacologia , Vitamina E/farmacologia , Animais , Quimioterapia Combinada , Enterite/metabolismo , Enterite/patologia , Glutationa/metabolismo , Glutationa Peroxidase/metabolismo , Glutationa Transferase/metabolismo , Íleo/metabolismo , Íleo/patologia , Peróxidos Lipídicos/metabolismo , Masculino , Lesões Experimentais por Radiação/metabolismo , Lesões Experimentais por Radiação/patologia , Ratos , Ratos Sprague-Dawley , Superóxido Dismutase/metabolismo , Substâncias Reativas com Ácido Tiobarbitúrico/metabolismo
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