Dynamic changes in monoterpene accumulation and biosynthesis during grape ripening in three Vitis vinifera L. cultivars.

Monoterpenes are important aroma components in grapes and wines. We analyzed the free and bound monoterpene profiles and the transcript levels of terpenoid biosynthesis genes in Vitis Vinifera cvs. Muscat Hamburg, Riesling, and Sauvignon Blanc grapes at five ripening stages. Principal component analyses revealed that the three cultivars had different free monoterpene profiles at harvest and the early stage of ripening. In all cultivars, the total bound monoterpene contents were higher than the free monoterpene contents during grape ripening. The changes in monoterpene profiles in different grape varieties were correlated with the transcript levels of some VviTPS and VviGT genes. In Riesling, the VviGT14 and VviUGT88A1L1 transcript levels were related to geraniol glucoside accumulation. In Muscat Hamburg, the VviPNLGl1, VviPNLGl2, and VviPNLGl4 transcript levels were related to linalool accumulation. Understanding the dynamic changes in monoterpene accumulation and biosynthesis will allow winemakers to devise strategies to improve grape and wine aromas.

Effect of cluster zone leaf removal on monoterpene profiles of Sauvignon Blanc grapes and wines.

Monoterpenes contribute to the varietal aromas of grapes and wines. We determined the effects of cluster zone leaf removal on the monoterpene profiles of Sauvignon Blanc grape berries and wines, and on the expression of key genes in the terpenoid pathway. Leaf removal at two intensities (half basic, 50%; full basic, 100%) was conducted at two weeks before veraison, veraison, and two weeks after veraison. Half basic leaf removal increased the pH and decreased the tartaric acid content in grapes and wines. The concentrations of most free- and bound-form monoterpenes in grapes were increased by early leaf removal. The total monoterpene contents were increased in wines in the defoliation treatments, but were significantly lower in wines from the full basic leaf removal treatments than in wines from the half basic leaf removal treatments. The defoliation treatments resulted in increased transcript levels of some key genes in terpene biosynthesis (VvPNLinNer1, VvPNLinNer2, VvPNLNGl1, VvPNLNGl2, and VvUGT88A1L1).