THE CHEWING LICE OF BROWN BOOBY (SULA LEUCOGASTER) IN SOUTHEASTERN BRAZIL.

The brown booby (Sula leucogaster Boddaert, 1783) has a wide geographic distribution, being found throughout the intertropical range except for the west coast of South America. Chewing lice (Phthiraptera: Amblycera and Ischnocera) are ectoparasites commonly found in association with a wide variety of bird species, and extensive infestations can lead to severe itching and irritation that causes skin lesions, damage to the plumage, and abandonment of nests. Sula leucogaster lice have an atypical distribution, not fully following the distribution of their host. In the years 2018 and 2019, 4 marine animal rehabilitation centers located in Rio de Janeiro and São Paulo, Brazil received live birds and carcasses of Sula leucogaster. The animals were deloused and lice of 2 different species were collected. Identification was performed by optical microscopy, and the species found were Eidmanniella albescens Piaget, 1880 and Pectinopygus garbeiPessoa and Guimarães, 1935. This is the first record of both species in Rio de Janeiro.

PRELIMINARY EXPERIMENTATIONS ON THE IN VITRO REARING OF HEMATOPHAGOUS PIGEON LOUSE HOHORSTIELLA LATA (AMBLYCERA: PHTHIRAPTERA: INSECTA).

Rearing hematophagous amblyceran lice in vitro is a challenging task. The hematophagous nature and active habits of amblycerans are distinct hurdles to in vitro rearing. The literature indicates only limited success in rearing the hematophagous amblyceran avian louse. Herein we report on the results of in vitro experimentation on an amblyceran pigeon louse, Hohorstiella lata. The incubation period of eggs was 5.47 ± 0.52 days. The durations of first, second, and third nymphal instars were 5.14 ± 0.55, 5.65 ± 0.83, and 6.35 ± 0.82 days, respectively. The average lifespan of adult females (7.45 ± 5.88 days) was higher than adult males (4.61 ± 3.57 days). Adult females laid a lifetime average of 3.73 eggs at a rate of 0.45 eggs/female/day under in vitro conditions (35 ± 1 C, 75-82% relative humidity, feather diet).

Diversity and host associations of Myrsidea chewing lice (Phthiraptera: Menoponidae) in the tropical rainforest of Malaysian Borneo.

The tropical rainforests of Sundaland are a global biodiversity hotspot increasingly threatened by human activities. While parasitic insects are an important component of the ecosystem, their diversity and parasite-host relations are poorly understood in the tropics. We investigated parasites of passerine birds, the chewing lice of the speciose genus MyrsideaWaterston, 1915 (Phthiraptera: Menoponidae) in a natural rainforest community of Malaysian Borneo. Based on morphology, we registered 10 species of lice from 14 bird species of six different host families. This indicated a high degree of host specificity and that the complexity of the system could be underestimated with the potential for cryptic lineages/species to be present. We tested the species boundaries by combining morphological, genetic and host speciation diversity. The phylogenetic relationships of lice were investigated by analyzing the partial mitochondrial cytochrome oxidase I (COI) and the nuclear elongation factor alpha (EF-1α) genes sequences of the species. This revealed a monophyletic group of Myrsidea lineages from seven hosts of the avian family Pycnonotidae, one host of Timaliidae and one host of Pellorneidae. However, species delimitation methods supported the species boundaries hypothesized by morphological studies and confirmed that four species of Myrsidea are not single host specific. Cophylogenetic analysis by both distance-based test ParaFit and event-based method Jane confirmed overall congruence between the phylogenies of Myrsidea and their hosts. In total we recorded three cospeciation events for 14 host-parasite associations. However only one host-parasite link (M. carmenae and their hosts Terpsiphone affinis and Hypothymis azurea) was significant after the multiple testing correction in ParaFit. Four new species are described: Myrsidea carmenae sp.n. ex Hypothymis azurea and Terpsiphone affinis, Myrsidea franciscae sp.n. ex Rhipidura javanica, Myrsidea ramoni sp.n. ex Copsychus malabaricus stricklandii, and Myrsidea victoriae sp.n. ex. Turdinus sepiarius.

Four new species of Myrsidea (Phthiraptera: Amblycera: Menoponidae) from Chinese babblers (Passeriformes: Leiothrichidae, Paradoxornithidae, Timaliidae).

Four new species of amblyceran chewing lice of the genus Myrsidea Waterston, 1915 are described from hosts of the babbler families Leiothrichidae, Paradoxornithidae and Timaliidae in China. They are: Myrsidea attenuata n. sp. from Garrulax maesi maesi (Oustalet, 1890), Myrsidea zhangae n. sp. from Ianthocincla berthemyi (Oustalet, 1876), Myrsidea liopari n. sp. from Lioparus chrysotis amoenus (Mayr, 1941) and L. chrysotis swinhoii (Verreaux, 1871), and Myrsidea suthorae n. sp. from Suthora verreauxi verreauxi Sharpe, 1883. A checklist of host-louse associations for identified and unidentified Myrsidea species known from babblers is provided.

Sex interacts with age-dependent change in the abundance of lice-infesting Amur Falcons (Falco amurensis).

Sex-biassed and age-biassed parasite infections are common in nature, including ectoparasites-vertebrate host systems. We investigated the effect of Amur Falcons' sex, age and body size on the abundance of their lice at a migratory stopover site, where the falcons' habitat use and behaviour are more homogeneous across sex and age categories than during the breeding season. We sampled Amur Falcons in Nagaland, India at major roosting sites in 2016. We applied generalized linear models (with negative binomial distribution and log-link) to model the abundance of their two most numerous lice (Colpocephalum subzerafae and Degeeriella rufa) using the host age category (juvenile or adult) and wing length, both in interaction with sex, as explanatory variables. The abundance of C. subzerafae was only affected by host age, being nearly four times higher on juveniles than on adults. Juveniles were also more infested with D. rufa than the adults. Additionally, the abundance of the latter species was lower on adult male Falcons as compared to adult females. A juvenile bias in ectoparasite infestations is common in nature, probably due to juveniles being immunologically naïve, more resource-limited and may be inexperienced in body maintenance behaviours like preening and grooming. On the other hand, female-biassed infestations are much rarer than male-biassed infestations. We briefly discuss the possible causes of female-biassed infestations on Amur Falcons reported here, and in the closely related Red-footed Falcon and Lesser Kestrel as reported in the literature.

Sex-dependent changes in the louse abundance of red-footed falcons (Falco vespertinus).

Permanent ectoparasites live in stable environments; thus, their population dynamics are mostly adapted to changes in the host life cycle. We aimed to investigate how static and dynamic traits of red-footed falcons interplay with the dynamics of their louse subpopulations during breeding and how they affect the colonisation of new hosts by lice. We sampled red-footed falcon (Falco vespertinus) nestlings (two breeding seasons) and adults (one breeding season) in southern Hungary. The mean abundance of Colpocephalum subzerafae and Degeeriella rufa lice on the nestlings was modelled with generalized linear mixed models using clutch size and host sex in interaction with wing length. For adults, we used wing length and the number of days after laying the first egg, both in interaction with sex. D. rufa abundances increased with the nestlings' wing length. In one year, this trend was steeper on females. In adult birds, both louse species exhibited higher abundances on females at the beginning, but it decreased subsequently through the breeding season. Contrarily, abundances were constantly low on adult males. Apparently, D. rufa postpones transmission until nestlings develop juvenile plumage and choose the more feathered individual among siblings. The sexual difference in the observed abundance could either be caused by the different plumage, or by the females' preference for less parasitized males. Moreover, females likely have more time to preen during the incubation period, lowering their louse burdens. Thus, sex-biased infestation levels likely arise due to parasite preferences in the nestlings and host behavioural processes in the adult falcons.

The Influence of Host Body Size and Food Guild On Prevalence and Mean Intensity of Chewing Lice (Phthiraptera) On Birds In Southern China.

Chewing lice (Insecta: Phthiraptera) are abundant ectoparasites of birds and mammals. They are adapted to life in the plumage or pelage of their hosts and virtually never leave the host during their life cycle. Most species are highly host specific. This study was carried out to determine species richness, abundance, and prevalence of chewing lice of wild forest birds in the southern region of China. Between July 2012 and June 2016, 2,210 birds (belonging to 8 orders, 45 families, and 215 species) were captured by mist nets and examined for chewing lice. In total, 622 birds of 117 species were parasitized by lice belonging to 89 species in 25 genera from 2 suborders (Amblycera and Ischnocera). Of these, 28 louse species represent new host-louse records for China and 10 worldwide. Chewing louse prevalence varied significantly among host species. There was no evidence of a correlation between climate zones and louse prevalence, but host guild affected prevalence significantly, with insectivorous birds having the lowest prevalence. Louse prevalence was positively correlated with host body mass and bill length, but mean intensity was only correlated with host body mass. These findings contribute further knowledge of avian chewing lice.

Chewing lice (Insecta: Phthiraptera) associated with vertebrates in Mexico.

The chewing lice (Insecta: Phthiraptera: Amblycera and Ischnocera) of Mexico have been little studied and many publications include isolated records. This paper summarizes current knowledge of chewing lice recorded from Mexico resulting from an exhaustive search of the literature published from 1866 to 2017. We found 342 louse species associated with 206 bird and 28 mammal species. As a result, we provide a checklist of the chewing lice recorded from Mexico, including a host-parasite list and their geographical distribution within the country.

Chewing lice from wild birds in northern Greece.

Greece represents an important area for wild birds due to its geographical position and habitat diversity. Although the bird species in Greece are well recorded, the information about the chewing lice that infest them is practically non-existent. Thus, the aim of the present study was to record the species of lice infesting wild birds in northern Greece and furthermore, to associate the infestation prevalence with factors such as the age, sex, migration and social behaviour of the host as well as the time of the year. In total 729 birds, (belonging to 9 orders, 32 families and 68 species) were examined in 7 localities of northern Greece, during 9 ringing sessions from June 2013 until October 2015. Eighty (11%) of the birds were found to be infested with lice. In 31 different bird species, 560 specimens of lice, belonging to 33 species were recorded. Mixed infestations were recorded in 11 cases where birds were infested with 2-3 different lice species. Four new host-parasite associations were recorded i.e. Menacanthus curuccae from Acrocephalus melanopogon, Menacanthus agilis from Cettia cetti, Myrsidea sp. from Acrocephalus schoenobaenus, and Philopretus citrinellae from Spinus spinus. Moreover, Menacanthus sinuatus was detected on Poecile lugubris, rendering this report the first record of louse infestation in this bird species. The statistical analysis of the data collected showed no association between parasitological parameters (prevalence, mean and median intensity and mean abundance) in two different periods of the year (breeding vs post-breeding season). However, there was a statistically significant difference in the prevalence of infestation between a) migrating and sedentary passerine birds (7.4% vs 13.2%), b) colonial and territorial birds (54.5% vs 9.6%), and c) female and male birds in breeding period (2.6% vs 15.6%).

A new species of the genus Amyrsidea (Phthiraptera: Amblycera: Menoponidae) parasitizing domestic chickens in Saudi Arabia.

Amyrsidea Ewing, 1927 is a genus of chewing lice comprising 52 species, divided into five subgenera: Amyrsidea sensu stricto, Argimenopon Eichler, 1947, Cracimenopon Carriker, 1954, Desumenopon Carriker, 1954 and Numidimenopon Scharf & Price, 1977 (see Price et al. 2003: 86), which parasitise a wide range of hosts belonging to the avian order Galliformes (see Price et al. 2003: 321). Species of Amyrsidea can be distinguished from species of Menacanthus Neumann, 1912-the other menoponid genus with species parasitic on the same hosts-by lacking ventral spinous processes in the head (Scharf & Price 1977: 815). Revisions of all subgenera and species of Amyrsidea, including keys for their identification, were published by Scharf & Price (1977, 1983) and Scharf & Emerson (1983, 1984).

A note on the haematophagous nature of poultry shaft louse, Menopon gallinae (Amblycera: Phthiraptera).

An analysis of the crop contents of the poultry shaft louse, Menopon gallina indicates that 67 % of adult males and 72 % of the adult females examined carried red content compatible to host blood, along with feather barbules. The percentage of nymphal instars carrying red content in the crop was quite low. On the basis of crop contents the louse does not seem to harbor any triturating agent and is also not involved in cannibalism or predation. SEM studies on the ventral side of head do not indicate the presence of any pointed structure which can be used to pierce the skin.

Rate of population increase of poultry shaft louse, Menopon gallinae in vivo condition.

Attempts were made to determine the rate of expansion of Menopon gallinae by releasing 10 healthier looking adult lice on each of the twelve similar aged fowls and delousing two fowls fortnightly (up to 90 days). The experiments were performed in the months of summer as well as in the months of winter. It was found that an initial inoculum of 10 M. gallinae could produce 636 lice in summers (doubling time 15 days). During winter months 323 M. gallinae were recovered from an inoculum of 10 lice (doubling time 18 days). Thus, summer months appear to be more favorable for the population build up of lice.

Walk or ride? Phoretic behaviour of amblyceran and ischnoceran lice.

Phoresy is a behaviour where one organism hitches a ride on another more mobile organism. This is a common dispersal mechanism amongst relatively immobile species that specialise on patchy resources. Parasites specialise on patchily distributed resources: their hosts. Although host individuals are isolated in space and time, parasites must transmit between hosts or they will die with their hosts. Lice are permanent obligate ectoparasites that complete their entire life cycle on their host. They typically transmit when hosts come into direct contact; however, lice are also capable of transmitting phoretically. Yet, phoresy is rare amongst some groups of lice. Fundamental morphological differences have traditionally been used to explain the phoretic differences amongst different suborders of lice; however, these hypotheses do not fully explain observed patterns. We propose that a more fundamental natural history trait may better explain variation in phoresy. Species able to disperse under their own power should be less likely to engage in phoresy than more immobile species. Here we experimentally tested the relationship between independent louse mobility and phoresy using a system with four species of lice (Phthiraptera: Ischnocera and Amblycera) that all parasitize a single host species, the Rock Pigeon (Columba livia). We quantified the relative ability of all four species of lice to move independently off the host, and we quantified their ability to attach to, and remain attached to, hippoboscid flies (Pseudolynchia canariensis). Our results show that the most mobile louse species is the least phoretic, and the most phoretic species is quite immobile off the host. Our findings were consistent with the hypothesis that phoretic dispersal should be rare amongst species of lice that are capable of independent dispersal; however other factors such as interspecific competition may also play a role.

Contribución al conocimiento de los malófagos (Phthiraptera, Amblycera, Ischnocera) de aves peruanas: Parte 1

En el presente trabajo se reporta nueve especies de malófagos colectados de aves peruanas. Dos malófagos Amblycera: Heteromenopon laticapitis y Menacanthus pici, así como siete malófagos Ischnocera: Alcedoffula theresae, Brueelia brunneinucha, Chelopistes meleagridis, Columbicola columbae, Paragoniocotes aratingae, Penenirmus jungens y Saemundssonia (Saemundssonia) lari, fueron determinados mediante el diagnóstico morfológico. Se realiza una breve descripción de cada malófago, así como la discusión de sus hospederos y distribución.

Nine species of Mallophaga from Peruvian birds are reported in this work. Two Mallophaga Amblycera: Heteromenopon laticapitis y Menacanthus pici, as well as seven Mallophaga Ischnocera: Alcedoffula theresae, Brueelia brunneinucha, Chelopistes meleagridis, Columbicola columbae, Paragoniocotes aratingae, Penenirmus jungens y Saemundssonia (Saemundssonia) lari, were identified morphologically. A brief description of each chewing lice and discussion about their hosts and distribution is done.

Population levels of Phthiraptera on domestic ducks (Anas platyrhynchos) (Anseriformes: Anatidae).

Three phthirapteran species (two Ischnocera and one Amblycera) were recovered from hundred ducks in district Bareilly and Rampur during 2011-2012. Prevalence of Anaticola crassicornis was comparatively higher (31 %) than that of Anatoecus dentatus (16 %) and Holomenpon leucoxanthum (28 %). However, the intensity of infestation of H. leucoxanthum (22.89) remained higher than the other two species. Distribution pattern of all lice were skewed but negative binomial model was not found to be a good fit. Sex ratios of all three species were skewed in favour of females (A. crassicornis-1:1.23, H. leucoxanthum-1:1.19, A. dentatus-1:1.72) and nymphal population exceeded the adult population (A. crassicornis-1:1.26, H. leucoxanthum-1:1.12, A. dentatus-1:1.61).

Prevalence of Myrsidea salimalii (Amblycera: Phthiraptera) on striated babblers (Turdoides earlei) (Timaliidae: Passeriformes: Aves).

Survey of literature showed that the population characteristics of the phthirapterans parasitizing striated babblers deserved investigation. Hence, 30 birds were examined during 2007-2009 in district Rampur U.P. The prevalence of an amblyceran louse, Myrsidea salimalii on striated babblers was 40%. The mean intensity of infestation and the sample mean abundance were 33.5 and 13.4, respectively. The variance to mean ratio of the population exceeded unity (36.24). The frequency distribution pattern of the louse was aggregated but did not conform to the negative binomial model. Females outnumbered the males in natural condition (M:F-1:1.4) while the nymphal population had an edge over adult population (A:N-1:1.3).

A survey of phthirapteran ectoparasites on the Grey Francolin, Francolinus Pondicerianus (Galliformes: phasianidae) in North-India.

As many as 144 brown francolin (Francolinus pondicerianus) were netted from 6 sites of Uttarakhand during the period from October, 2006 to September, 2008, to note the occurrence of phthirapteran species. Out of 144 birds, 45.83% were found to be infested with two species of lice i.e. Menacanthus kalatitar and Goniocotes jirufi, the former species occurring in 29.17% of birds (mean intensity 11.45 and sample mean abundance 3.34) whereas the later infesting only 25.69% of birds (mean intensity 8.22 and sample mean abundance 2.11). Significant positive correlation existed between mean monthly lice index and mean monthly temperature as well as photoperiod, while negative correlation was seen with respect to relative humidity and rainfall.