Enhanced photorespiratory and TCA pathways by elevated CO2 to manage ammonium nutrition in tomato leaves.

Plants grown under exclusive ammonium (NH4+) nutrition have high carbon (C) demand to sustain proper nitrogen (N) assimilation and energy required for plant growth, generally impaired when compared to nitrate (NO3-) nutrition. Thereby, the increment of the atmospheric carbon dioxide (CO2) concentration, in the context of climate change, will potentially allow plants to better face ammonium nutrition. In this work, tomato (Solanum lycopersicum L.) plants were grown under ammonium or nitrate nutrition in conditions of ambient (aCO2, 400 ppm) or elevated CO2 (eCO2, 800 ppm) atmosphere. Elevated CO2 increased photosynthesis rate and tomato shoot growth regardless of the N source. In the case of NH4+-fed leaves the positive effect of elevated CO2 occurred despite of the high tissue NH4+ accumulation. Under eCO2 ammonium nutrition triggered, among others, the modulation of genes related to C provision pathways (including carbonic anhydrase and glyoxylate cycle), antioxidant response and cell membranes protection. The enhanced photosynthate production at eCO2 facilitated C skeleton provision through the TCA cycle and anaplerotic pathways to promote amino acid synthesis. Moreover, photorespiratory activity was stimulated by eCO2 and contributed to yield serine as additional sink for NH4+ excess. Overall, these changes denote a connection between the respiratory and the photorespiratory pathways linked to ammonium nutrition. This metabolic strategy may allow crops to grow efficiently using ammonium as fertilizer in a future climate change scenario, while mitigating N losses.

Elevated pCO2 may increase the edible safety risk of clams exposed to toxic Alexandrium spp.

Toxic harmful algal blooms (HABs) have received increasing attention owing to their threat to the health of aquatic life and seafood consumers. This study evaluated the impacts of elevated atmospheric partial pressure of CO2 (pCO2) on the production of paralytic shellfish toxins (PSTs) in different Alexandrium spp. strains, together with its further effects on the bioaccumulation/elimination dynamics of PSTs in bivalves contaminated with PSTs from toxic dinoflagellates. Our results showed that elevated pCO2 stimulated the growth of the two Alexandrium spp. (A. catenella and A. pacificum) isolated from the northern and southern coastal areas of China, respectively, and affected PST production including content and toxicity of the two strains differently. Further PSTs bioaccumulation/elimination in PSTs-contaminated Manila clam, Ruditapes philippinarum under high pCO2 also occurred. It is worth noting the biotransformation of neosaxitoxin (NEO) with high toxicity through trophic transfer with effect of elevated pCO2. When in microalgae cultured under the control (410 ppm) and elevated pCO2 conditions (495 and 850 ppm), the proportion of NEO in the PST content produced by A. catenella was reduced from 11.1 to 6.4 and 2.6 %, while the proportion of NEO in A. pacificum was increased from 3.1 to 3.6 and 4.7 %, respectively. NEO accounted for >50 % of total PST contents in clams, which were biotransformed via transfer from dinoflagellates and higher pCO2 enhanced this biotransformation leading to increased NEO accumulation. The negatively affected elimination of PSTs, especially NEO, in clams fed with A. catenella or A. pacificum, indicates that the detoxification of PSTs-contaminated clams may be more difficult under elevated pCO2. This study provides reference for developing models to assess the safety of bivalves under the co-stress of environmental change and toxic HABs, suggesting that ocean acidification may lead to the higher safety risk of Manila clams exposed to toxic HAB dinoflagellates.

Elevated concentrations of soil carbon dioxide with partial root-zone drying enhance drought tolerance and agro-physiological characteristics by regulating the expression of genes related to aquaporin and stress response in cucumber plants.

Water scarcity and soil carbon dioxide elevation in arid regions are considered the most serious factors affecting crop growth and productivity. This study aimed to investigate the impacts of elevated CO2 levels (eCO2 at rates of 700 and 1000 ppm) on agro-physiological attributes to induce drought tolerance in cucumbers by activating the expression of genes related to aquaporin and stress response, which improved the yield of cucumber under two levels of irrigation water conditions [75% and 100% crop evapotranspiration (ETc)]. Therefore, two field experiments were conducted in a greenhouse with controlled internal climate conditions, at the Mohamed Naguib sector of the national company for protected agriculture, during the winter seasons of 2021-2022 and 2022-2023. The treatments included eCO2 in soil under normal and partial root zoon drying (PRD, 100% ETc Full irrigations, and 75% ETc). All the applied treatments were organized as a randomized complete block design (RCBD) and each treatment was replicated six times. Untreated plants were designed as control treatment (CO2 concentration was 400 ppm). The results of this study showed that elevating CO2 at 700 and 1000 ppm in soil significantly increased plant growth parameters, photosynthesis measurements, and phytohormones [indole acetic acid (IAA) and gibberellic acid (GA3)], under partial root-zone drying (75% ETc) and full irrigation conditions (100% ETc). Under PRD condition, eCO2 at 700 ppm significantly improved plant height (13.68%), number of shoots (19.88%), Leaf greenness index (SPAD value, 16.60%), root length (24.88%), fresh weight (64.77%) and dry weight (61.25%) of cucumber plant, when compared to untreated plants. The pervious treatment also increased photosynthesis rate, stomatal conductance, and intercellular CO2 concentration by 50.65%, 15.30% and 12.18%; respectively, compared to the control treatment. Similar findings were observed in nutrient concentration, carbohydrate content, Proline, total antioxidants in the leaf, and nutrients. In contrast, eCO2 at 700 ppm in the soil reduced the values of transpiration rate (6.33%) and Abscisic acid (ABA, 34.03%) content in cucumber leaves compared to untreated plants under both water levels. Furthermore, the results revealed that the gene transcript levels of the aquaporin-related genes (CsPIP1-2 and CsTIP4) significantly increased compared with a well-watered condition. The transcript levels of CsPIP improved the contribution rate of cell water transportation (intermediated by aquaporin's genes) and root or leaf hydraulic conductivity. The quantitative real-time PCR expression results revealed the upregulation of CsAGO1 stress-response genes in plants exposed to 700 ppm CO2. In conclusion, elevating CO2 at 700 ppm in the soil might be a promising technique to enhance the growth and productivity of cucumber plants in addition to alleviating the adverse effects of drought stresses.

Elevated CO2 enhances growth and cyanide assimilation in nitrogen-deficient rice: A transcriptome and metabolomic perspective.

Plants face multiple challenges from environmental pollutants and higher emissions of atmospheric CO2. Therefore, a hydroponic-based experiment was used to explore the combined effects of elevated [CO2] (700 ppm) and exogenous cyanide (CN-) (3.0 mg CN/L) on rice seedlings under nitrogen deficiency, utilizing metabonomic and transcriptomic analysis. Elevated [CO2] significantly improved the growth of CN--treated rice seedlings compared to those with ambient [CO2] (350 ppm), and it also significantly affected CN- assimilation. Transcriptome analysis revealed distinct impacts on differentially expressed genes (DEGs) across treatments and tissues. KEGG analysis showed variability in DEGs enriched in amino acid (AA) and energy metabolism pathways due to elevated [CO2] and CN-. Metabonomic indicated that higher input of [CO2] and exogenous CN- more severely impacted energy metabolism elements than the individual species of AAs. Positive synergistic effects of elevated [CO2] and CN- were observed for glutamine and asparagine in shoots, and methionine in roots, wherein negative effects were noted for phenylalanine in shoots, and phenylalanine, valine, and alanine in roots. Meanwhile, positive effects on fumarate in shoots and α-ketoglutarate and succinate in roots were also found. Overall, elevated [CO2] enhanced growth in CN--treated rice seedlings under nitrogen deficiency by altering AA and energy metabolism. This is the first attempt to provide new evidence of [CO2]-based gaseous fertilization as an energy-saving strategy for rice plants fed with biodegradable N-containing pollutants as a supporting N source under N deficient conditions.

Lower grass stomatal conductance under elevated CO2 can decrease transpiration and evapotranspiration rates despite carbon fertilization.

Anthropogenic increase in carbon dioxide (CO2) affects plant physiology. Plant responses to elevated CO2 typically include: (1) enhanced photosynthesis and increased primary productivity due to carbon fertilization and (2) suppression of leaf transpiration due to CO2-driven decrease in stomatal conductance. The combined effect of these responses on the total plant transpiration and on evapotranspiration (ET) has a wide range of implications on local, regional, and global hydrological cycles, and thus needs to be better understood. Here, we investigated the net effect of CO2-driven perennial ryegrass (Lolium perenne) physiological responses on transpiration and evapotranspiration by integrating physiological and hydrological (water budget) methods, under a controlled environment. Measurements of the net photosynthetic rate, stomatal conductance, transpiration rate, leaf mass per area, aboveground biomass, and water balance components were recorded. Measured variables under elevated CO2 were compared with those of ambient CO2. As expected, our results show that elevated CO2 significantly decreases whole-plant transpiration rates (38% lower in the final week) which is a result of lower stomatal conductance (57% lower in the final week) despite a slight increase in aboveground biomass. Additionally, there was an overall decline in evapotranspiration (ET) under elevated CO2, indicating the impact of CO2-mediated suppression of transpiration on the overall water balance. Although studies with larger sample sizes are needed for more robust conclusions, our findings have significant implications for global environmental change. Reductions in ET from ryegrass-dominated grasslands and pastures could increase soil moisture and groundwater recharge, potentially leading to increased surface runoff and flooding.

Plant growth-promoting rhizobacteria enhance active ingredient accumulation in medicinal plants at elevated CO2 and are associated with indigenous microbiome.

Introduction: Plant growth-promoting rhizobacteria (PGPR) and elevated CO2 (eCO2) have demonstrated their individual potential to enhance plant yield and quality through close interaction with rhizosphere microorganisms and plant growth. However, the efficacy of PGPR under eCO2 on rhizosphere microbiome and, ultimately, plant yield and active ingredient accumulation are not yet fully understood. Methods: This study investigated how the medicinal plant Pseudostellaria heterophylla (P. heterophylla) and its rhizosphere microbes respond to PGPR (Bacillus subtilis and Pseudomonas fluorescens) at eCO2 (1,000 ppm). Results and Discussion: It was found that the yield and active ingredient polysaccharides accumulation in the tuber of P. heterophylla were significantly increased by 38 and 253%, respectively. This promotion has been associated with increased root development and changes in the indigenous microbial community. Metagenomics analysis revealed a significant reduction in pathogenic Fusarium abundance in the rhizosphere. Potential biocontrol bacteria Actinobacteria and Proteobacteria were enriched, especially the genera Bradyrhizobium and Rhodanobacter. The reshaping of the rhizosphere microbiome was accompanied by the upregulation of biological pathways related to metabolite biosynthesis in the rhizosphere. These modifications were related to the promotion of the growth and productivity of P. heterophylla. Our findings highlighted the significant role played by PGPR in medicinal plant yield and active ingredient accumulation when exposed to eCO2.

Does the response of Rubisco and photosynthesis to elevated [CO2] change with unfavourable environmental conditions?

Climate change due to anthropogenic CO2 emissions affects plant performance globally. To improve crop resilience, we need to understand the effects of elevated CO2 concentration (e[CO2]) on CO2 assimilation and Rubisco biochemistry. However, the interactive effects of e[CO2] and abiotic stress are especially unclear. This study analyses the CO2 effect on photosynthetic capacity under different water availability and temperature conditions in 42 different crop species, varying in functional group, photosynthetic pathway and phenological stage. We analysed close to 3000 data points extracted from 120 published manuscripts. For C3 species, e[CO2] increases net photosynthesis and intercellular [CO2], while reducing stomatal conductance and transpiration. Vmaxc, Rubisco in vitro extractable maximal activity and content also decrease with e[CO2] in C3 species, while C4 crops are less responsive to e[CO2]. The interaction with drought and/or heat stress does not significantly alter these photosynthetic responses, indicating that the photosynthetic capacity of stressed plants responds to e[CO2]. Moreover, e[CO2] has strong effect on the photosynthetic capacity of grasses mainly in the final stages of development. This study provides insight into the intricate interactions within the plant photosynthetic apparatus under the influence of climate change, enhancing the understanding of mechanisms governing plant responses to environmental parameters.

Understanding the impact of elevated CO2 and O3 on growth and yield in Indian wheat cultivars: Implications for food security in a changing climate.

The pressing issue of increasing tropospheric ozone (O3) levels necessitates the development of effective stress management strategies for plant protection. While considerable research has elucidated the adverse impacts of O3, understanding the combined effects of O3 and CO2 requires further investigation. This study focuses on assessing the response of stomatal O3 flux under various O3 and CO2 treatments, individually and in combination, and their repercussions on physiological, growth, and yield attributes in two Indian wheat cultivars, HUW-55 and PBW-550, which exhibit varying levels of sensitivities against elevated O3. Results indicated significant alterations in stomatal O3 flux in both O3-sensitive and tolerant wheat cultivars across different treatments, influencing the overall yield outcomes. Particularly, the ECO2+EO3 treatment demonstrated more positive yield protection in the O3-sensitive cultivar PBW-550, compared to HUW-55 indicating enhanced allocation of photosynthates towards reproductive development in PBW-550, compared to the tolerant cultivar HUW-55, as evidenced by higher harvest index (HI). Furthermore, the study revealed a stronger correlation between yield response and stomatal O3 flux in PBW-550 (R2 = 0.88) compared to HUW-55 (R2 = 0.79), as indicated by a steeper regression slope for PBW-550. The research also confirmed the role of elevated CO2 in reducing stomatal O3- flux in the tested cultivars, with discernible effects on their respective yield responses. Further experimentation is necessary to confirm these results across different cultivars exhibiting varying sensitivities to O3. These findings can potentially revolutionize agricultural productivity in regions affected by O3 stress. The criteria for recommending cultivars for agricultural practices should not be based only on their sensitivity/tolerance to O3. Still, they should also consider the effect of CO2 fertilization in the growing area. This experiment offers hope to sustain global food security, as the O3-sensitive wheat cultivar also showed promising results at elevated CO2. In essence, this research could pave the way for more resilient agricultural systems in the era of changing climate under elevated O3 and CO2 conditions.

Effect of CO2 Elevation on Tomato Gas Exchange, Root Morphology and Water Use Efficiency under Two N-Fertigation Levels.

Atmospheric elevated CO2 concentration (e[CO2]) decreases plant nitrogen (N) concentration while increasing water use efficiency (WUE), fertigation increases crop nutrition and WUE in crop; yet the interactive effects of e[CO2] coupled with two N-fertigation levels during deficit irrigation on plant gas exchange, root morphology and WUE remain largely elusive. The objective of this study was to explore the physiological and growth responses of ambient [CO2] (a[CO2], 400 ppm) and e[CO2] (800 ppm) tomato plant exposed to two N-fertigation regimes: (1) full irrigation during N-fertigation (FIN); (2) deficit irrigation during N-fertigation (DIN) under two N fertilizer levels (reduced N (N1, 0.5 g pot-1) and adequate N (N2, 1.0 g pot-1). The results indicated that e[CO2] associated with DIN regime induced the lower N2 plant water use (7.28 L plant-1), maintained leaf water potential (-5.07 MPa) and hydraulic conductivity (0.49 mol m-2 s-1 MPa-1), greater tomato growth in terms of leaf area (7152.75 cm2), specific leaf area (223.61 cm2 g-1), stem and total dry matter (19.54 g and 55.48 g). Specific root length and specific root surface area were increased under N1 fertilization, and root tissue density was promoted in both e[CO2] and DIN environments. Moreover, a smaller and denser leaf stomata (4.96 µm2 and 5.37 mm-2) of N1 plant was obtained at e[CO2] integrated with DIN strategy. Meanwhile, this combination would simultaneously reduce stomatal conductance (0.13 mol m-2 s-1) and transpiration rate (1.91 mmol m-2 s-1), enhance leaf ABA concentration (133.05 ng g-1 FW), contributing to an improvement in WUE from stomatal to whole-plant scale under each N level, especially for applying N1 fertilization (125.95 µmol mol-1, 8.41 µmol mmol-1 and 7.15 g L-1). These findings provide valuable information to optimize water and nitrogen fertilizer management and improve plant water use efficiency, responding to the potential resource-limited and CO2-enriched scenario.

Effect of copper and temperature on the photosynthetic physiological characteristics of Ulva linza under elevated CO2 concentrations.

Copper (Cu) is vital for macroalgae's functions, but high concentrations can be toxic. Rising CO2 levels affect algal growth and Cu bioavailability. In this study, the results reveal that at 5 °C, low Cu increased Ulva linza growth, while high Cu and elevated CO2 decreased growth. At 10 °C, low Cu and elevated CO2 enhanced growth, but high Cu did not have a significant impact. At 15 °C, high Cu reduced growth, but elevated CO2 offset this effect. Furthermore, under elevated CO2 conditions, the chloroplast structure of the algae appeared to be denser, accompanied by a large amount of starch granules, compared to low CO2 conditions. These results emphasize that lower temperatures, in conjunction with elevated CO2 concentration, could intensify the toxic effects of high Cu concentrations on thalli. However, at higher temperatures, elevated CO2 concentration appeared to be capable of mitigating the detrimental effects of heavy metals on algae.

Impact of elevated CO2 on microbial communities and functions in riparian sediments: Role of pollution levels in modulating effects.

The impact of elevated CO2 levels on microorganisms is a focal point in studying the environmental effects of global climate change. A growing number of studies have demonstrated the importance of the direct effects of elevated CO2 on microorganisms, which are confounded by indirect effects that are not easily identified. Riparian zones have become key factor in identifying the environmental effects of global climate change because of their special location. However, the direct effects of elevated CO2 levels on microbial activity and function in riparian zone sediments remain unclear. In this study, three riparian sediments with different pollution risk levels of heavy metals and nutrients were selected to explore the direct response of microbial communities and functions to elevated CO2 excluding plants. The results showed that the short-term effects of elevated CO2 did not change the diversity of the bacterial and fungal communities, but altered the composition of their communities. Additionally, differences were observed in the responses of microbial functions to elevated CO2 levels among the three regions. Elevated CO2 promoted the activities of nitrification and denitrification enzymes and led to significant increases in N2O release in the three sediments, with the greatest increase of 76.09 % observed in the Yuyangshan Bay (YYS). Microbial carbon metabolism was promoted by elevated CO2 in YYS but was significantly inhibited by elevated CO2 in Gonghu Bay and Meiliang Bay. Moreover, TOC, TN, and Pb contents were identified as key factors contributing to the different microbial responses to elevated CO2 in sediments with different heavy metal and nutrient pollution. In conclusion, this study provides in-depth insights into the responses of bacteria and fungi in polluted riparian sediments to elevated CO2, which helps elucidate the complex interactions between microbial activity and environmental stressors.

Responses of Brassica napus to soil cadmium under elevated CO2 concentration based on rhizosphere microbiome, root transcriptome and metabolome.

Rising atmospheric carbon dioxide (CO2) and soil heavy metal pollution affect crop safety and production. Exposure to elevated CO2 (ECO2) increases cadmium (Cd) uptake in some crops like wheat and rice, however, it remains unclear how ECO2 affects Cd uptake by Brassica napus. Here, we investigated the responses of B. napus seedlings exposed to ECO2 and Cd through analyses of physiology, transcriptome, metabolome, and rhizosphere microbes. Compared with Cd-stress alone (Cd50_ACO2), ECO2 boosted the uptake of Cd by B. napus roots by 38.78% under coupled stresses (Cd50_ECO2). The biomass and leaf chlorophyll a content increased by 38.49% and 79.66% respectively in Cd50_ECO2 relative to Cd50_ACO2. Activities of superoxide dismutase (SOD) and peroxidase (POD) enhanced by 8.42% and 185.01%, respectively, while glutathione (GSH) and ascorbic acid (AsA) contents increased by 16.44% and 52.48%, and abundances of rhizosphere microbes changed significantly under coupled stresses (Cd50_ECO2) relative to Cd-stress alone (Cd50_ACO2). Also, the upregulation of glutathione, glutathione transferase genes, and heavy metal ATPase expression promoted the detoxification effect of rapeseed on Cd. Changes in the expression of transcription factors like MAPK, WRKY, BAK1 and PR1, as well as changes in metabolic pathways like ß-alanine, may be involved in the regulatory mechanism of stress response. These findings provide new insights for studying the regulatory mechanism of rapeseed under ECO2 on soil Cd stress, and also provide a basis for further research on Cd tolerant rapeseed varieties in the future climate context.

Climate change does not impact the water flow of barley at the vegetative stage, ameliorates at anthesis and worsens after subsequent drought episodes.

Climate change will bring the interaction of stresses such as increased temperature and drought under high [CO2] conditions. This is likely to impact on crop growth and productivity. This study aimed to (i) determine the response of barley water relations to vegetative and anthesis drought periods under triple interaction conditions, (ii) test the possibility to prime barley plants for drought, and (iii) analyse the involvement of aquaporins in (i) and (ii). The water status of barley was not affected by drought at the vegetative stage, regardless of the environmental conditions. At the anthesis stage, when the water shortage period was more severe, barley plants growing under combined elevated CO2 and temperature conditions were able to maintain a better water status compared with plants grown under current conditions. Elevated CO2 and temperature conditions reduced the stomatal conductance and slowed down the plant water flow through a root-leaf hydraulic conductivity coordination. Leaf HvPIP2;1 and HvTIP1;1 aquaporins seemed to play a key role regulating barley's water flow, while leaf and root HvPIP2;5 provided basic level of water flow. At anthesis drought and under future combined conditions, plants showed a reduced cell dehydration and decrease in leaf relative water content compared with plants grown under current conditions. Exposure to a previous drought did not prime the water status of barley plants to a subsequent drought, but instead worsened the response under future conditions. This was due to an imbalance between the roots versus shoot development.

Improving hydrological modeling to close the gap between elevated CO2 concentration and crop response: Implications for water resources.

Rising atmospheric carbon dioxide concentrations ([CO2]) affect crop growth and the associated hydrological cycle through physiological forcing, which is mainly regulated by reducing stomatal conductance (gs) and increasing leaf area index (LAI). However, reduced gs and increased LAI can affect crop water consumption, and the overall effects need to be quantified under elevated [CO2]. Here we develop a SWAT-gs-LAI model by incorporating a nonlinear gs-CO2 equation and a missing LAI-CO2 relationship to investigate the responses of water consumption of grain maize, maize yield, and losses of water and soil to elevated [CO2] in the Upper Mississippi River Basin (UMRB; 492,000 km2). Results exhibited enhanced maize yield with decreased water consumption for increases in [CO2] from 495 ppm to 825 ppm during the historical period (1985-2014). Elevated [CO2] promoted surface runoff but suppressed sediment loss as the predominant impact of LAI-CO2 leading to enhanced surface cover. A comprehensive analysis of future climate change showed increased maize water consumption in comparison to the historical period, driven by the more pronounced effects of overall climate change rather than solely elevated [CO2]. Generally, future climate change promoted maize yield in most regions of the UMRB for three Shared Socioeconomic Pathway (SSP) scenarios. Surface runoff was shown to increase generally in the future with sediment loss increasing by an average of 0.39, 0.42, and 0.66 ton ha-1 for SSP1-2.6, SSP2-4.5, and SSP5-8.5, respectively. This was due to negative climatic change effects largely surpassing the positive effect of elevated [CO2], particularly in zones near the middle and lower stream. Our results underscore the crucial role of employing a physically-based model to represent crop physiological processes under elevated [CO2] conditions, improving the reliability of predictions related to crop growth and the hydrological cycle.

Resistance of grassland productivity to drought and heatwave over a temperate semi-arid climate zone.

Drought and heatwave are the primary climate extremes for vegetation productivity loss in the global temperate semi-arid grassland, challenging the ecosystem productivity stability in these areas. Previous studies have indicated a significant decline in the resistance of global grassland productivity to drought, but we still lack a systematic understanding of the mechanisms determining the spatiotemporal variations in grassland resistance to drought and heatwave. In this study, we focused on temperate semi-arid grasslands of China (TSGC) to assess the spatiotemporal variations of grassland productivity resistance to different climate extremes: compound dry-hot events, individual drought events, and individual heatwave events that occurred during 2000-2019. Based on the explainable machine learning model, we explored the resistance to the interaction of drought and heatwave and identify the dominant factors determining the spatiotemporal variations in resistance. The results revealed that grassland resistance to climate extremes had decreased in Xilingol Grassland and Mu Us Sandy Land, and had a not significant increase in Otindag Desert during 2000-2019. Human activities and the increase in CO2 concentration causes a decline in resistance in Mu Us Sandy Land, and the increase of VPD and shift of vegetation loss event timing caused a decline in resistance in Xilingol Grassland, while the weakening of climate extremes, especially the shortening of drought duration, increase the resistance in Otindag Desert. Mean annual temperature dominates the spatial differences in resistance among different grasslands. When drought and heatwave occur simultaneously, there is an additive effect on resistance and causes lower resistance to compound dry-hot events compared to individual drought and heatwave events. Our analysis provides crucial insights into understanding the impact of climate extremes on the temperate semi-arid grasslands of China.

Effect of biochar amendment on bacterial community and their role in nutrient acquisition in spinach (Spinacia oleracea L.) grown under elevated CO2.

Global climate change is anticipated to shift the soil bacterial community structure and plant nutrient utilization. The use of biochar amendment can positively influence soil bacterial community structure, soil properties, and nutrient use efficiency of crops. However, little is known about the underlying mechanism and response of bacterial community structure to biochar amendment, and its role in nutrient enhancement in soil and plants under elevated CO2. Herein, the effect of biochar amendment (0, 0.5, 1.5%) on soil bacterial community structure, spinach growth, physiology, and soil and plant nutrient status were investigated under two CO2 concentrations (400 and 600 µmol mol-1). Findings showed that biochar application 1.5% (B.2.E) significantly increased the abundance of the bacterial community responsible for growth and nutrient uptake i.e. Firmicutes (42.25%) Bacteroidetes (10.46%), and Gemmatimonadetes (125.75%) as compared to respective control (CK.E) but interestingly abundance of proteobacteria decreased (9.18%) under elevated CO2. Furthermore, the soil available N, P, and K showed a significant increase in higher biochar-amended treatments under elevated CO2. Spinach plants exhibited a notable enhancement in growth and photosynthetic pigments when exposed to elevated CO2 levels and biochar, as compared to ambient CO2 conditions. However, there was variability observed in the leaf gas exchange attributes. Elevated CO2 reduced spinach roots and leaves nutrient concentration. In contrast, the biochar amendment (B2.E) enhanced root and shoot Zinc (494.99%-155.33%), magnesium (261.15%-183.37%), manganese (80.04%-152.86%), potassium (576.24%-355.17%), calcium (261.88%-165.65%), copper (325.42%-282.53%) and iron (717.63%-177.90%) concentration by influencing plant physiology and bacterial community. These findings provide insights into the interaction between plant and bacterial community under future agroecosystems in response to the addition of biochar contributing to a deeper understanding of ecological dynamics.

Biochemical versus stomatal acclimation of dynamic photosynthetic gas exchange to elevated CO2 in three horticultural species with contrasting stomatal morphology.

Understanding photosynthetic acclimation to elevated CO2 (eCO2) is important for predicting plant physiology and optimizing management decisions under global climate change, but is underexplored in important horticultural crops. We grew three crops differing in stomatal density-namely chrysanthemum, tomato, and cucumber-at near-ambient CO2 (450 µmol mol-1) and eCO2 (900 µmol mol-1) for 6 weeks. Steady-state and dynamic photosynthetic and stomatal conductance (gs) responses were quantified by gas exchange measurements. Opening and closure of individual stomata were imaged in situ, using a novel custom-made microscope. The three crop species acclimated to eCO2 with very different strategies: Cucumber (with the highest stomatal density) acclimated to eCO2 mostly via dynamic gs responses, whereas chrysanthemum (with the lowest stomatal density) acclimated to eCO2 mostly via photosynthetic biochemistry. Tomato exhibited acclimation in both photosynthesis and gs kinetics. eCO2 acclimation in individual stomatal pore movement increased rates of pore aperture changes in chrysanthemum, but such acclimation responses resulted in no changes in gs responses. Although eCO2 acclimation occurred in all three crops, photosynthesis under fluctuating irradiance was hardly affected. Our study stresses the importance of quantifying eCO2 acclimatory responses at different integration levels to understand photosynthetic performance under future eCO2 environments.

Rare earth elements as a tool to study the foliar nutrient uptake phenomenon under ambient and elevated atmospheric CO2 concentration.

The ability of plants to uptake nutrients from mineral dust lying on their foliage may prove to be an important mechanism by which plants will cope with increasing CO2 levels in the atmosphere. This mechanism had only recently been reported and was shown to compensate for the projected dilution in plants ionome. However, this phenomenon has yet to be thoroughly studied, particularly in terms of the expected trends under different dust types and varying atmospheric CO2 concentrations, as projected by the IPCC. We treated plants grown under ambient (415 ppm) and elevated CO2 (850 ppm) conditions with either desert dust, volcanic ash, and fire ash analogues by applying it solely on plant foliage and studied their Rare Earth Elements concentrations and patterns. The Rare Earth Elements compositions of the treated plants originated from the dust application, and their incorporation into the plants led to a significant increase in plants vitality, evident in increased photosynthetic activity and biomass. Two trends in the foliar nutrient uptake mechanism were revealed by the Rare Earth Elements, one is that different treatments affected the plant in decreasing order volcanic ash > desert dust > fire ash. The second trend is that foliar intake becomes more significant under elevated CO2, an observation not previously seen. This testifies that the use of Rare Earth Elements in the study of foliar nutrient uptake, and other biological mechanisms is fundamental, and that foliar pathways of nutrient uptake will indeed become more dominant with increasing CO2 under expected atmospheric changes.