Insects to the rescue? Insights into applications, mechanisms, and prospects of insect-driven remediation of organic contaminants.

Traditional and emerging contaminants pose significant human and environmental health risks. Conventional physical, chemical, and bioremediation techniques have been extensively studied for contaminant remediation. However, entomo- or insect-driven remediation has received limited research and public attention. Entomo-remediation refers to the use of insects, their associated gut microbiota, and enzymes to remove or mitigate organic contaminants. This novel approach shows potential as an eco-friendly method for mitigating contaminated media. However, a comprehensive review of the status, applications, and challenges of entomo-remediation is lacking. This paper addresses this research gap by examining and discussing the evidence on entomo-remediation of various legacy and emerging organic contaminants. The results demonstrate the successful application of entomo-remediation to remove legacy organic contaminants such as persistent organic pollutants. Moreover, entomo-remediation shows promise in removing various groups of emerging contaminants, including microplastics, persistent and emerging organic micropollutants (e.g., antibiotics, pesticides), and nanomaterials. Entomo-remediation involves several insect-mediated processes, including bio-uptake, biotransfer, bioaccumulation, and biotransformation of contaminants. The mechanisms underlying the biotransformation of contaminants are complex and rely on the insect gut microbiota and associated enzymes. Notably, while insects facilitate the remediation of contaminants, they may also be exposed to the ecotoxicological effects of these substances, which is often overlooked in research. As an emerging field of research, entomo-remediation has several knowledge gaps. Therefore, this review proposes ten key research questions to guide future perspectives and advance the field. These questions address areas such as process optimization, assessment of ecotoxicological effects on insects, and evaluation of potential human exposure and health risks.

The Variety of Applications of Hermetia illucens in Industrial and Agricultural Areas-Review.

Hermetia illucens (Diptera: Stratiomyidae, Linnaeus, 1978), commonly known as the black soldier fly (BSF), is a saprophytic insect, which in recent years has attracted significant attention from both the scientific community and industry. The unrestrained appetite of the larvae, the ability to forage on various organic waste, and the rapid growth and low environmental impact of its breeding has made it one of the insect species bred on an industrial scale, in the hope of producing fodder or other ingredients for various animals. The variety of research related to this insect has shown that feed production is not the only benefit of its use. H. illucens has many features and properties that could be of interest from the point of view of many other industries. Biomass utilization, chitin and chitosan source, biogas, and biodiesel production, entomoremediation, the antimicrobial properties of its peptides, and the fertilizer potential of its wastes, are just some of its potential uses. This review brings together the work of four years of study into H. illucens. It summarizes the current state of knowledge and introduces the characteristics of this insect that may be helpful in managing its breeding, as well as its use in agro-industrial fields. Knowledge gaps and under-studied areas were also highlighted, which could help identify future research directions.

Biodegradation of Different Types of Plastics by Tenebrio molitor Insect.

Looking for new, sustainable ways to utilize plastics is still a very pertinent topic considering the amount of plastics produced in the world. One of the newest and intriguing possibility is the use of insects in biodegradation of plastics, which can be named entomoremediation. The aim of this work was to demonstrate the ability of the insect Tenebrio molitor to biodegrade different, real plastic waste. The types of plastic waste used were: remains of thermal building insulation polystyrene foam (PS), two types of polyurethane (kitchen sponge as PU1 and commercial thermal insulation foam as PU2), and polyethylene foam (PE), which has been used as packaging material. After 58 days, the efficiency of mass reduction for all of the investigated plastics was 46.5%, 41.0%, 53.2%, and 69.7% for PS, PU1, PU2, and PE, respectively (with a dose of 0.0052 g of each plastic per 1 mealworm larvae). Both larvae and imago were active plastic eaters. However, in order to shorten the duration of the experiment and increase the specific consumption rate, the two forms of the insect should not be combined together in one container.

A New Approach to Quantifying Bioaccumulation of Elements in Biological Processes.

Bioaccumulation, expressed as the bioaccumulation factor (BAF), is a phenomenon widely investigated in the natural environment and at laboratory scale. However, the BAF is more suitable for ecological studies, while in small-scale experiments it has limitations, which are discussed in this article. We propose a new indicator, the bioaccumulation index (BAI). The BAI takes into account the initial load of test elements, which are added to the experimental system together with the biomass of the organism. This offers the opportunity to explore the phenomena related to the bioaccumulation and, contrary to the BAF, can also reveal the dilution of element concentration in the organism. The BAF can overestimate bioaccumulation, and in an extremal situation, when the dilution of element concentration during organism growth occurs, the BAF may produce completely opposite results to the BAI. In one of the examples presented in this work (Tschirner and Simon, 2015), the concentration of phosphorous in fly larvae was lower after the experiment than in the younger larvae before the experiment. Because the phosphorous concentration in the feed was low, the BAF indicated a high bioaccumulation of this element (BAF = 14.85). In contrast, the BAI showed element dilution, which is a more realistic situation (BAI = -0.32). By taking more data into account, the BAI seems to be more valid in determining bioaccumulation, especially in the context of entomoremediation research.

Liminal presence of exo-microbes inoculating coconut endosperm waste to enhance black soldier fly larval protein and lipid.

The anaerobic decomposition of coconut endosperm waste (CEW), residue derived from cooking, has been insidiously spewing greenhouse gasses. Thus, the bioconversion of CEW via in situ fermentation by exo-microbes from commercial Rid-X and subsequent valorization by black soldier fly larvae (BSFL) was the primary objective of the current study to gain sustainable larval lipid and protein. Accordingly, various concentrations of exo-microbes were separately homogenized with CEW to perform fermentation amidst feeding to BSFL. It was found that 2.50% of exo-microbes was the threshold amount entailed to assuage competition between exo-microbes and BSFL for common nutrients. The presence of remnant nutrients exuded from the fermentation using 2.50% of exo-microbes was confirmed to promote BSFL growth measured as maximum larval weight gained and growth rate. Although the BSFL could accumulate the highest protein (16 mg/larva) upon feeding with CEW containing 2.50% of exo-microbes, more lipid (13 mg/larva) was stored in employing 0.10% of exo-microbes because of minimum loss to metabolic processes while prolonging the BSFL in its 5th instar stage.

Hermetia illucens as a new and promising species for use in entomoremediation.

This study investigated the use of Hermetia illucens (black soldier fly - BSF) larvae as a new species for use in entomoremediation. The H. illucens larvae effectively reduced the dry mass of polluted corn leaves by an average of 49% after 36days for both Cd and Zn (50mg·kg-1), which is a better result than that, which can be obtained by one of the standard proposed pretreatments for biomass polluted after phytoextraction: composting. The presence of heavy metals did not significantly affect the dry mass utilization efficiency. Based on this, we proposed the use of H. illucens as a new post-harvest management method of phytoextraction-polluted biomass. Cadmium mostly accumulates in the puparium, while Zn accumulates in the adults. The high Cd content in the puparium further creates possibility of its application in the metal recovery process. It is also proposed that the general definition of entomoremediation be expanded.