Ethnomedicinal survey and mutagenic studies of plants used in Accra metropolis, Ghana.

ETHNOPHARMACOLOGICAL RELEVANCE: Majority of people living in Ghana and many other developing countries rely on traditional medicinal plants for their primary healthcare. These plants are used either alone or in combination to manage a wide range of ailments. However, most of these plants have not been investigated for their mutagenic effects. AIM OF THE STUDY: This study, therefore aimed at evaluating the mutagenic activity of the most frequently used medicinal plants amongst Ghanaians living within the Accra metropolis, Ghana. MATERIALS AND METHODS: Validated questionnaires were administered to 53 herbalists and herbal medicines dealers in the Makola, Madina and Nima communities. Plants that were identified as being frequently used were investigated for their mutagenicity using the Ames test. RESULTS: A total of 110 medicinal plants belonging to 53 families were identified as most frequently used plants in the study sites. These are used to treat various ailments including gastric ulcer, fever, malaria, male impotence, diabetes, typhoid, high blood pressure and candidiasis. Thirteen samples (52%) showed moderate to high mutagenicity in the TA 100 bacterial strain before and after metabolism with rat liver enzyme. CONCLUSIONS: The study showed that over half of the frequently used medicinal plants showed moderate to high mutagenicity before and after metabolism at the concentration of a 100 µg/mL. This may have implications for the safety of those who use them to manage diseases. These findings will suggest the need for an in-depth study of the mutagenic potentials of plants commonly used by indigenous people and more especially for those exhibiting high mutagenicity in this study.

Ethnomedicinal survey of plants used in the treatment of malaria in Southern Nigeria.

ETHNOPHARMACOLOGICAL RELEVANCE: Malaria is one of the most severe public health problems worldwide. It is a leading cause of death and disease in many developing countries, where young children and pregnant women are the groups most affected. Spread of multidrug-resistant strains of Plasmodium and the adverse side effects of the existing anti-malarial drugs have necessitated the search for novel, well tolerated and more efficient antimalarial drugs. This ethnomedicinal study surveyed the different types of medicinal plants used for the treatment of malaria in Southern Nigeria with the intent of identifying plants that are traditionally employed in the treatment of malaria across geopolitical boundaries. MATERIALS AND METHODS: Data were collected from 79 respondents composed of 50 traditional herbsellers and 29 herbal practitioners using a semi-structured questionnaire. Data was analyzed using frequency and percentages. RESULTS: Of the 79 respondents interviewed, 24% were males while 76% were females. A total of 156 species belonging to 60 families were reported being used to treat malaria in the study area. Fabaceae was the most represented family having fourteen (14) plant species. Of the plants identified during the survey, Azadirachta indica was the species of highest relative frequency of citation (RFC - 1.0). The dominant plant parts used in the preparation of remedies were leaves (50.50%) and Decoction was the main method of preparation. Analysis of regional plant occurrence revealed that South-Western Nigeria represented the region with the highest plant occurrence (60.7%) followed by South-South (24%) and South-East (15.3%). Regional occurrence of plants used in the treatment of malaria in Southern Nigeria is reported here for the first time. CONCLUSION: This study has documented a great diversity of plants used in the treatment of malaria in Southern Nigeria. Extracts prepared strictly according to the practitioners' recipes should therefore be screened for antiplasmodial activity and toxicity by in vitro and in vivo standard tests to justify their local usage. These studies might lead to the isolation and possible identification of potentially active compounds, which may be regarded as future promising phytomedicines in the treatment of malaria. Conservation of these plant species is also recommended to ensure their continuous availability for future use.