Genome-resolved metagenomics reveals the nitrifiers enrichment and species succession in activated sludge under extremely low dissolved oxygen.

Nitrification, a process carried out by aerobic microorganisms that oxidizes ammonia to nitrate via nitrite, is an indispensable step in wastewater nitrogen removal. To facilitate energy and carbon savings, applying low dissolved oxygen (DO) is suggested to shortcut the conventional biological nitrogen removal pathway, however, the impact of low DO on nitrifying communities within activated sludge is not fully understood. This study used genome-resolved metagenomics to compare nitrifying communities under extremely low- and high-DO. Two bioreactors were parallelly operated to perform nitrification and DO was respectively provided by limited gas-liquid mass transfer from the atmosphere (AN reactor, DO < 0.1 mg/L) and by sufficient aeration (AE reactor, DO > 5.0 mg/L). Low DO was thought to limit nitrifiers growth; however, we demonstrated that complete nitrification could still be achieved under the extremely low-DO conditions, but with no nitrite accumulation observed. Kinetic analysis showed that after long-term exposure to low DO, nitrifiers had a higher oxygen affinity constant and could maintain a relatively high nitrification rate, particularly at low levels of DO (<0.2 mg/L). Community-level gene analysis indicated that low DO promoted enrichment of nitrifiers (the genera Nitrosomonas and Nitrospira, increased by 2.3- to 4.3-fold), and also harbored with 2.3 to 5.3 times higher of nitrification functional genes. Moreover, 46 high-quality (>90 % completeness and <5 % contamination) with 3 most abundant medium-quality metagenome-assembled genomes (MAGs) were retrieved using binning methods. Genome-level phylogenetic analysis revealed the species succession within nitrifying populations. Surprisingly, compared to DO-rich conditions, low-DO conditions were found to efficiently suppressed the ordinary heterotrophic microorganisms (e.g., the families Anaerolineales, Phycisphaerales, and Chitinophagales), but selected for the specific candidate denitrifiers (within phylum Bacteroidota). This study provides new microbial insights to demonstrate that low-DO favors the enrichment of autotrophic nitrifiers over heterotrophs with species-level successions, which would facilitate the optimization of energy and carbon management in wastewater treatment.

Effects of hypoxia on the reproductive endocrine axis of the pejerrey (Odontesthes bonariensis).

Recently, hypoxic areas have been identified in water bodies of the Pampas region due to human activity. The objective of this work was to study the effect of low concentrations of dissolved oxygen (hypoxia) on the reproductive endocrine axis of a pampas fish (Odontesthes bonariensis). Groups of 8 males and 8 females were subjected to severe hypoxia (2-3 mg l-1) and normoxia (7-9 mg l-1) in 3000 l tanks by duplicate during the reproductive season (spring). After 21 days, 4 males and 4 females from each tank were sacrificed, and blood was drawn to measure estradiol (E2) and testosterone (T). The brain, pituitary gland and a portion of the gonads were extracted and processed to measure the expression of: gnrh1, cyp19a1b, fshß, lhß, fshr, lhcgr and cyp19a1a. From the second experimental week, no spawning was found in the hypoxic females, while at the end of the treatment period no male released sperm. Fish under hypoxic conditions showed signs of gonadal regression, reduction of GSI and plasma levels of sex steroids. Furthermore, the expression of gnrh1 in both sexes, cyp19a1b and fshr in males and only fshß and cyp19a1a in females decreased in comparison with normoxic fish. After 40 days under normal conditions, signs of reproductive recovery were observed in the treated fish. The results obtained demonstrated that hypoxia generated an inhibition of some components of the pejerrey's reproductive endocrine axis, but the effect was reversible.

A novel simultaneous short-course nitrification, denitrification and fermentation process: bio-enhanced phenol degradation and denitrification in a single reactor.

The feasibility of a simultaneous nitrification, denitrification and fermentation process (SNDF) under electric stirrer agitation conditions was verified in a single reactor. Enhanced activated sludge for phenol degradation and denitrification in pharmaceutical phenol-containing wastewater under low dissolved oxygen conditions, additional inoculation with Comamonas sp. BGH and optimisation of co-metabolites were investigated. At a hydraulic residence time (HRT) of 28 h, 15 mg/L of substrate as strain BGH co-metabolised substrate degraded 650 ± 50 mg/L phenol almost completely and was accompanied by an incremental increase in the quantity of strain BGH. Strain BGH showed enhanced phenol degradation. Under trisodium citrate co-metabolism, strain BGH combined with activated sludge treated phenol wastewater and degraded NO2--N from 50 ± 5 to 0 mg/L in only 7 h. The removal efficiency of this group for phenol, chemical oxygen demand (COD) and TN was 99.67%, 90.25% and 98.71%, respectively, at an HRT of 32 h. The bioaugmentation effect not only promotes the degradation of pollutants, but also increases the abundance of dominant bacteria in activated sludge. Illumina MiSeq sequencing research showed that strain BGH promoted the growth of dominant genera (Acidaminobacter, Raineyella, Pseudarcobacter) and increased their relative abundance in the activated sludge system. These genera are resistant to toxicity and organic matter degradation. This paper provides some reference for the activated sludge to degrade high phenol pharmaceutical wastewater under the action of biological enhancement.

Metagenomics insights into high-rate nitrogen removal from municipal wastewater by integrated nitrification, partial denitrification and Anammox at an extremely short hydraulic retention time.

To achieve high-rate nitrogen removal in municipal wastewater treatment through anaerobic ammonia oxidation (Anammox), the nitrification, partial denitrification, and Anammox processes were integrated by a step-feed strategy. An exceptional nitrogen removal load of 0.224 kg N/(m3·d) was achieved by gradient-reducing the hydraulic retention time (HRT) to 5 h. Metagenomic analysis demonstrated that Nitrosospira could express all genes encoding ammonia oxidation under low nitrogen and dissolved oxygen conditions (less than 0.5 mg/L), enabling complete nitrification. With the short of HRT, the relative abundance of Thauera increased from 2.8 % to 6.4 %. Frequent substrate exchanges at such extremely short HRT facilitated enhanced synergistic interactions among Nitrosospira, Thauera, and Candidatus Brocadia. These findings provide a comprehensive understanding of the utilization of Anammox combined processes for high-speed nitrogen removal in municipal wastewater treatment and the microbial interactions involved.

Pilot-scale study of step-feed anaerobic coupled four-stage micro-oxygen gradient aeration process for treating digested swine wastewater with low carbon/nitrogen ratios.

This study developed an innovative step-feed anaerobic coupled four-stage micro-oxygen gradient aeration process to treat digested swine wastewater. An anaerobic zone was used for prepositive denitrification; four micro-oxygen reactors (zones O1-O4) were used for simultaneous partial nitrification and denitrification through low-dissolved oxygen gradient control, step-feed, and swine wastewater-digested swine wastewater distribution. The nitrogen-removal efficiency was satisfactory (93 ± 3 %; effluent total nitrogen, 53 ± 19 mg/L). Mass balance coupled with quantitative polymerase chain reaction analysis revealed that simultaneous partial nitrification and denitrification was achieved in four micro-oxygen zones. Zones O1 were the major denitrification zones for nitrogen removal; nitrification was primary happened in zones O2 and O3. Correlation analysis confirmed that low-dissolved oxygen gradient control was the key to achieving efficient nitrogen removal. This study provides a low oxygen energy consumption method to treat digested swine wastewater with a low carbon/nitrogen ratio (<3).

Robust nitrogen removal from municipal wastewater by partial nitrification anammox at ultra-low dissolved oxygen in a pure biofilm system.

Efficient nitrogen removal from municipal wastewater applying a pure biofilm system has promise. In this study, a partial nitrification anammox (PNA) pure biofilm system was established in a sequencing batch reactor with anaerobic/oxic/anoxic operation; using this reactor, robust nitrogen removal from municipal wastewater at ambient temperature was achieved with a nitrogen removal efficiency (NRE) of 93.3 %. Partial nitrification with anammox could be coupled at dominant nitrite-oxidizing bacteria (NOB) abundance by controlling ultra-low dissolved oxygen (<0.1 mg/L) in the aerobic section where the contribution to nitrogen removal was 79.4 %. Microorganisms with different oxygen affinity spatially distributed on the carrier. Ammonia-oxidizing bacteria (AOB) dominated on the surface of the carrier, while anammox bacteria dominated on the interior of the carrier, with their relative abundance increasing from 0.26 % to 1.78 %. The intercalary NOB were inhibited by the restricted oxygen transfer. Overall, this study provides a new approach to realize PNA in biofilm system.

Combining continuous flow aerobic granulation using an external selector and carbon-efficient nutrient removal with AvN control in a full-scale simultaneous nitrification-denitrification process.

The James R. Dolorio Water Reclamation Facility in Pueblo, Colorado, uses AvN aeration controls to lower aeration energy while promoting carbon-efficient nutrient removal and hydrocyclone-based wasting to achieve SVI improvements and process intensification. The results from the full-scale installation showed that hydrocyclone-based wasting helped improve settling characteristics by reducing the SVI from 200 ± 52 mL/g to 83 ± 22 mL/g within weeks of operation. PAO and nitrifiers were preferentially retained in dense flocs and granules, while lighter heterotrophic and filamentous organisms were preferentially wasted, thus uncoupling the SRT of these two fractions relative to the overall SRT. The SRT was estimated at 14.4 ± 3.4 days for dense aggregates and 7.1 ± 2.3 days for lighter flocs. The use of AvN control with continuous low DO conditions resulted in low DO conditions (< 0.3 mgO2/L) reducing air demand by 50% while providing excellent nitrogen (effluent TIN < 11 mgN/L) and TP removal (effluent TP < 1 mgP/L) at low primary effluent COD/N ratio of 6.0. The presence of comammox was demonstrated through molecular analysis, while ex-situ batch tests revealed the presence of DPAO, which could have attributed to the energy and carbon-efficient biological nutrient removal.

A novel simultaneous partial nitritation, denitratation and anammox (SPNDA) process in sequencing batch reactor for advanced nitrogen removal from ammonium and nitrate wastewater.

This study presented a novel simultaneous partial nitritation (PN), denitratation and anammox (SPNDA) process for treating ammonium and nitrate wastewater. Results indicated that SPNDA could achieve a great total nitrogen (TN) removal of 97.6 ± 0.5%, leading to effluent TN concentration of only 3.4 mg/L. Mass balance indicated that nitrogen removal rates via anammox, simultaneous nitrification and denitrification were 96.7% and 3.3%, respectively. Extended aerobic duration (12 h) and low dissolved oxygen (DO) concentration (0.15 mg/L) could improve ammonia-oxidizing bacteria (AOB) activity and maintain PN stability. The stable suppression of nitrite-oxidizing bacteria activity was attributed to the low DO (0.15 mg/L) and high free ammonia (3.63 mg/L) in SPND. Besides, the nitrogen conversion mechanisms for SPNDA were revealed based on a typical operational cycle. Microbial analysis showed that AOB (Nitrosomonas) and partial denitrifying bacteria (Thauera and Denitratisoma) coexisted with anammox bacteria (Candidatus Brocadia and Candidatus Anammoxoglobus) in the mixotrophic bio-community.

Influence of Riverbed Incision and Hydrological Evolution on Water Quality and Water Age Based on Numerical Simulation: A Case Study of the Minjiang Estuary.

In recent years, problems such as water quality deterioration, saltwater invasion, and low oxygen have appeared in estuaries all over the world. The Minjiang River in Fujian, as a typical tidal estuary area, is facing these thorny problems. In this paper, the effects of topography and hydrologic evolution on the water age and water quality of the lower reaches of the Minjiang River were simulated by building a hydrodynamic and water quality model. The results show that: (1) It was found that the riverbed incision of the lower reaches of the Minjiang River led to the overall decline of river water level, the increase of river volume, and the increase of downstream water age, which eventually led to the decrease of dissolved oxygen (DO) and the deterioration of water quality in the downstream from Shuikou to Baiyantan. However, the decline of topography led to the increase of tidal volume in the estuary, the enhancement of the dilution effect of oxygen-rich water bodies in the open sea, and the increase of DO in the lower reaches of Baiyantan. (2) Under no tidal action, the concentration of pollutants in the water of the North Channel increased, the DO decreased, and the DO decreased from Baiyantan to the offshore water. After the enhancement of tidal action, the dilution of oxygen-enriched water from the offshore water increased, and the DO increased. (3) The hydrological and water quality characteristics of the upper part of the lower reaches of the Minjiang River were mainly controlled by topography, runoff, and pollutant discharge, which were more affected by the tidal current transport operation and pollutant discharge near the open sea. In recent decades, the deterioration of water quality and the aggravation of saltwater intrusion in the Minjiang River were closely related to the serious topographic downcutting. The results provide a scientific basis for revealing the deterioration of estuary water quality and long-term management of the estuary.

Superior nitrogen removal and sludge reduction in a suspended sludge system with in-situ enriching anammox bacteria for real sewage treatment.

Mainstream partial anammox provides a cost- and energy-efficient alternative for wastewater treatment. This study provided a new strategy to achieve mainstream partial anammox in a single-stage suspended sludge system. The novel method coupling external excess sludge fermentation with simultaneous partial nitritation-anammox-denitrification process (SF-SPNAD) was established for 202 days in an anaerobic-aerobic-anoxic sequencing batch reactor (AOA-SBR) with real sewage and actual sludge fermentation products. Under the condition of low DO (0.6 ± 0.2 mg/L), short oxic and long anoxic hydraulic retention time (HRToxic = 6.5 h, HRTanoxic = 8 h), the average total inorganic nitrogen (TIN) concentration in the influent and effluent during 110-day operation were 61.0 and 3.4 mg/L, respectively, and the TIN removal efficiency was 94.56%. Under the inhibitory effect of continuous sludge fermentation products addition, nitrite accumulation ratio reached 99.1% and the external sludge reduction ratio reached 38.75%. 15N-stable isotope tracing tests showed the great potential of nitrogen removal by anammox pathway in the system. High-throughput sequencing confirmed that CandidatusBrocadia (not detected to 0.50%) and CandidatusKuenenia (not detected to 0.06%) were successfully in-situ enriched. Nitrogen conversion pathways based on stoichiometry and cycle tests show that 34.69% of the TIN removal was obtained by simultaneous nitritation denitrification and anammox under oxic stage and 35.21% of the TIN removal was carried out by anammox under anoxic stage. Overall, the SF-SPNAD process provides a new possibility for coupling autotrophic and heterotrophic nitrogen removal with excess sludge utilization.

Influence of activated sludge dissolved oxygen concentration on a membrane bioreactor performance with intermittent aeration.

This study measured the effect of low activated sludge dissolved oxygen (DO) concentration on a membrane bioreactor (MBR) treating real urban wastewater with respect to organic matter and nitrogen removal efficiency and transmembrane pressure evolution. For this purpose, a full-scale experimental pre-denitrification MBR system was operated at a constant permeate flow rate of Q = 0.45 m3h-1 with intermittent aeration. The experimental installation worked at high hydraulic retention time, variable sludge retention time and with activated sludge temperatures of between 22.0 to 31.3 °C. Mean DO concentrations in the activated sludge were gradually decreased from 1.25 mgO2L-1 to less than 0.20 mgO2L-1. Variations in DO set points did not affect the main operational parameters of the MBR system and no clear relation was shown between DO concentration decrease and membrane fouling. At DO concentrations lower than 0.2 mgO2L-1, a deterioration in MBR effluent quality was observed, mainly with respect to chemical oxygen demand, biochemical oxygen demand at five days and NH4+, however, the opposite effect was observed for NO3-. These results indicate that employing low DO set points is a promising strategy for application in MBR systems.

Long-Term Low Dissolved Oxygen Operation Decreases N2O Emissions in the Activated Sludge Process.

Nitrous oxide (N2O) is an important greenhouse gas and a dominant ozone-depleting substance. Nitrification in the activated sludge process (ASP) is an important N2O emission source. This study demonstrated that a short-term low dissolved oxygen (DO) increased the N2O emissions by six times, while long-term low DO operation decreased the N2O emissions by 54% (P < 0.01). Under long-term low DO, the ammonia oxidizer abundance in the ASP increased significantly, and thus, complete nitrification was recovered and no NH3 or nitrite accumulated. Moreover, long-term low DO decreased the abundance of ammonia-oxidizing bacteria (AOB) by 28%, while increased the abundance of ammonia-oxidizing archaea (AOA) by 507%, mainly due to their higher oxygen affinity. As a result, AOA outnumbered AOB with the AOA/AOB amoA gene ratio increasing to 19.5 under long-term low DO. The efficient nitrification and decreased AOB abundance might not increase N2O production via AOB under long-term low DO conditions. The enriched AOA could decrease the N2O emissions because they were reported to lack canonical nitric oxide (NO) reductase genes that convert NO to N2O. Probably because of AOA enrichment, the positive and significant (P = 0.02) correlation of N2O emission and nitrite concentration became insignificant (P = 0.332) after 80 days of low DO operation. Therefore, ASPs can be operated with low DO and extended sludge age to synchronously reduce N2O production and carbon dioxide emissions owing to lower aeration energy without compromising the nitrification efficiency.

A novel pilot-scale IFAS-MBR system with low aeration for municipal wastewater treatment: Linkages between nutrient removal and core functional microbiota.

In this study, we proposed a novel IFAS-MBR with low aeration for the treatment of real municipal wastewater. With biocarriers packed in the anoxic tank, the pilot-scale IFAS-MBR operated with average dissolved oxygen concentrations of 0.56 mg/L in the oxic tank. Over 110 days of operation, highly efficient nutrient removal was achieved with the total nitrogen (TN) and phosphorus (TP) removal efficiencies of 78.1 ± 7.2% and 93.7 ± 5.8%, respectively. The average effluent concentrations of TN and TP reached 5.4 and 0.26 mg/L, respectively. Meanwhile, the removal efficiency of COD reached 95.3 ± 1.3% in the system, and the concentrations of COD decreased from 31.9 ± 3.7 (sludge supernatant) to 12.7 ± 1.6 mg/L (permeate) after membrane filtration. Microbial community analysis showed that Nitrosomonas (0.32%) and Nitrospira (1.85%) in activated sludge were the main drivers of the nitrification process, while various denitrifying bacteria in activated sludge and biofilms were responsible for nitrate reduction in the anoxic tank. Candidatus Accumulibacter (0.34%) and Dechloromonas (1.31%) primarily contributed to denitrifying phosphorus uptake in the anoxic tank. Furthermore, these organisms (i.e., core functional microbiota) exhibited stable levels over the entire operation. The highly enriched hydrolytic fermentation bacteria drove community succession, and the remarkable functional robustness of microbial communities in activated sludge and biofilms favored nutrient removal. Overall, the novel IFAS-MBR system provides an energy-efficient MBR alternative owing to its highly efficient performance and low operating costs enabled by low aeration rates and the absence of an external carbon source.

Enhanced biological nitrogen removal under low dissolved oxygen in an anaerobic-anoxic-oxic system: Kinetics, stoichiometry and microbial community.

A lab-scale anaerobic-anoxic-oxic system was used to investigate the nitrogen removal mechanism under low dissolved oxygen (DO) conditions. When DO was decreased from 2 to 0.5 mg L-1, chemical oxygen demand (COD) and NH4+ removals were not influenced, while total nitrogen removal increased from 69% to 79%. Further batch tests indicated that both the specific nitrification rate and denitrification rate greatly increased under low DO conditions. When DO was decreased from 2 to 0.5 mg L-1, the oxygen half saturation constant value for ammonia oxidizing bacteria (AOB) decreased from 0.39 to 0.29 mg-O2 L-1, and for nitrite oxidizing bacteria (NOB), it reduced from 0.29 to 0.09 mg-O2 L-1. Correspondingly, the observed yield coefficients increased from 0.05 to 0.10 mg-cell mg-1-N for AOB, and from 0.02 to 0.06 mg-cell mg-1-N for NOB. High-throughput sequencing revealed that the relative abundances of AOB increased from 6.13% to 6.54%, Nitrospira-like NOB increased from 3.67% to 6.50%, and denitrifiers increased from 2.84% to 7.04%. Improved simultaneous nitrification and denitrification under low DO conditions contributed to the enhanced nitrogen removal.

Effects of hypoxia on the behavior and physiology of kelp forest fishes.

Forecasts from climate models and oceanographic observations indicate increasing deoxygenation in the global oceans and an elevated frequency and intensity of hypoxic events in the coastal zone, which have the potential to affect marine biodiversity and fisheries. Exposure to low dissolved oxygen (DO) conditions may have deleterious effects on early life stages in fishes. This study aims to identify thresholds to hypoxia while testing behavioral and physiological responses of two congeneric species of kelp forest fish to four DO levels, ranging from normoxic to hypoxic (8.7, 6.0, 4.1, and 2.2 mg O2 /L). Behavioral tests identified changes in exploratory behavior and turning bias (lateralization), whereas physiological tests focused on determining changes in hypoxia tolerance (pCrit), ventilation rates, and metabolic rates, with impacts on the resulting capacity for aerobic activity. Our findings indicated that copper rockfish (Sebastes caurinus) and blue rockfish (Sebastes mystinus) express sensitivity to hypoxia; however, the strength of the response differed between species. Copper rockfish exhibited reduced absolute lateralization and increased escape time at the lowest DO levels, whereas behavioral metrics for blue rockfish did not vary with oxygen level. Both species exhibited decreases in aerobic scope (as a function of reduced maximum metabolic rate) and increases in ventilation rates to compensate for decreasing oxygen levels. Blue rockfish had a lower pCrit and stronger acclimation response compared to copper rockfish. The differences expressed by each species suggest that acclimatization to changing ocean conditions may vary, even among related species that recruit to the same kelp forest habitat, leading to winners and losers under future ocean conditions. Exposure to hypoxia can decrease individual physiological fitness through metabolic and aerobic depression and changes to anti-predator behavior, with implications for the outcome of ecological interactions and the management of fish stocks in the face of climate change.

Interactive effects of mercury exposure and hypoxia on ECG patterns in two Neotropical freshwater fish species: Matrinxã, Brycon amazonicus and traíra, Hoplias malabaricus.

Hypoxia and mercury contamination often co-occur in tropical freshwater ecosystems, but the interactive effects of these two stressors on fish populations are poorly known. The effects of mercury (Hg) on recorded changes in the detailed form of the electrocardiogram (ECG) during exposure to progressive hypoxia were investigated in two Neotropical freshwater fish species, matrinxã, Brycon amazonicus and traíra, Hoplias malabaricus. Matrinxã were exposed to a sublethal concentration of 0.1 mg L-1 of HgCl2 in water for 96 h. Traíra were exposed to dietary doses of Hg by being fed over a period of 30 days with juvenile matrinxãs previously exposed to HgCl2, resulting in a dose of 0.45 mg of total Hg per fish, each 96 h. Both species showed a bradycardia in progressive hypoxia. Hg exposure impaired cardiac electrical excitability, leading to first-degree atrioventricular block, plus profound extension of the ventricular action potential (AP) plateau. Moreover, there was the development of cardiac arrhythmias and anomalies such as occasional absence of QRS complexes, extra systoles, negative Q-, R- and S-waves (QRS complex), and T wave inversion, especially in hypoxia below O2 partial pressures (PO2) of 5.3 kPa. Sub-chronic dietary Hg exposure induced intense bradycardia in normoxia in traira, plus lengthening of ventricular AP duration coupled with prolonged QRS intervals. This indicates slower ventricular AP conduction during ventricular depolarization. Overall, the data indicate that both acute waterborne and sub-chronic dietary exposure (trophic level transfer), at sublethal concentrations of mercury, cause damage in electrical stability and rhythm of the heartbeat, leading to myocardial dysfunction, which is further intensified during hypoxia. These changes could lead to impaired cardiac output, with consequences for swimming ability, foraging capacity, and hence growth and/or reproductive performance.

Simultaneous partial nitritation and denitritation coupled with polished anammox for advanced nitrogen removal from low C/N domestic wastewater at low dissolved oxygen conditions.

Simultaneous partial nitritation and denitritation (SPND) coupled with anammox was established in this study to treat domestic wastewater. Two lab-scale bioreactors, namely SPND-SBR and ANA-UASB, were used in the two-stage system. In SPND-SBR, stable nitrogen removal efficiency of 51.1% was achieved with a high ammonia oxidation rate of 0.117 kg N/(m3·d). Besides, successful out-selection of nitrite-oxidizing bacteria (NOB) under low-DO of 0.1 mg/L during the steady period, resulting in an average effluent NO2--N/NH4+-N ratio of 1.04. In ANA-UASB, the abundance of Candidatus Brocadia and Candidatus Kuenenia increased from 8.21% and 4.01% to 21.33% and 6.41% with low influent substrate contents of only 38 mg N/L. The effluent total inorganic nitrogen (TIN) was only 8.4 ± 1.1 mg N/L and the nitrogen removal efficiency reached 88.24%. Overall, the study demonstrated that the novel low-DO two-stage process for nitrogen removal is a promising technique for wastewater of low C/N ratio.

Advanced nitrogen removal from municipal wastewater via two-stage partial nitrification-simultaneous anammox and denitrification (PN-SAD) process.

A modified two-stage anammox process was constructed and achieved advanced nitrogen removal from municipal wastewater. The first stage was Partial Nitrification (PN), in which nitrite accumulation rate was over 95% by controlling dissolved oxygen concentration (<1 mg/L) and aeration time (90-120 min). The second stage was simultaneous anammox and denitrification (SAD), in which the reactor was fed with the effluent of the first stage and a part of raw wastewater. The effluent total inorganic nitrogen (NH4+-N, NO2--N and NO3--N) was only 1.6 ± 0.8 mg N/L and the nitrogen removal efficiency reached 97.1%. The proportion of anammox in nitrogen removal was up to 73-82% and Candidatus Brocadia was the main anammox genus accounted for 8.0-2.2%. And partial denitrification occurred with the appearance of Thauera (0-1.0%). The PN-SAD process is an energy-saving treatment for municipal wastewater with a total hydraulic retention time of 6 h.

A pilot-scale study on the treatment of landfill leachate by a composite biological system under low dissolved oxygen conditions: Performance and microbial community.

In this work, a pilot-scale low dissolved oxygen (DO) composite biological system (LDOCBS) composed of an anoxic rotating biological contactor (RBC) and four aeration tanks with gradient aeration was used to treat landfill leachate for 88 d. The maximum removals of 85.65%, 99.92% and 84.06% for chemical oxygen demand (COD), ammonia (NH4+-N) and total nitrogen (TN) were achieved, respectively. The three-dimensional exaction and emission matrix (3D-EEM) fluorescence spectroscopy revealed that the biodegradability of leachate was significantly improved by the LDOCBS. Mass balance calculations showed that the COD removal and denitrification process mainly occurred in RBC while 1# contributed primarily to nitrification. High-throughput sequencing analysis indicated that denitrifying bacteria with highly relative abundances of 46.45%-53.81% played key roles in organic degradation and nitrogen removal. This work could add some guiding insights into the cost-efficient treatment of landfill leachate by the composite biological system.

Effects of low-intensity ultrasound on nitrite accumulation and microbial characteristics during partial nitrification.

Ultrasound technology has attracted increasing attention in the field of sewage sludge treatment. This study investigated the nitrite accumulation ratio (NAR) and microbial characteristics of the partial nitrification (PN) process in a sequencing batch reactor employing ultrasonic treatment (ultrasound density = 0.25 W/mL, irradiation time = 10 min). PN was achieved over 73 days with a NAR above 85% under ambient temperatures. A low dissolved oxygen (DO) environment was generated in the reactor by enhancing the oxygen utilization rate of ammonia-oxidizing bacteria (AOB). Additionally, the application of long-term ultrasonic treatment led to the enhancement of the dominance of the Nitrosomonas genus of AOB, while populations of the Nitrospira genus of nitrite-oxidizing bacteria (NOB) were eradicated. At the same time, the activities of the aerobic denitrifying bacteria Thauera, Terrimonas, Defluviimonas, and Thermomonas were enhanced and their relative abundance was increased. Overall, the results suggest that ultrasonic treatment can enhance AOB activity and generate a low DO environment that facilitates effective PN.