Biochemical, toxicological, and microbiological assessment of calcined poultry manure for potential use as bone scaffold material.

The biosafety of thermally calcined poultry manure as a hydroxyapatite source for potential use as bone-making material was investigated in this study. In vitro assays were used to determine the sensitivity of the antioxidant properties to the thermal calcination temperature used to process the poultry manure (750, 800, and 850 °C ). The effect of the extract of both calcined poultry manure (local) and analytical grade hydroxyapatite (foreign) at various concentrations of 100%-25 % inclusion at (100 mg/kg) body weight intubation for 21 days on kidney, liver, and serum of animal model used was assessed. The results show that the thermally calcined poultry manure-derived hydroxyapatite generally possessed good antioxidant properties with the poultry manure treated at 750 °C having the most promising antioxidant properties compared to those treated at 800 and 850 °C , and hence a more likely improved anti-toxicity potential. The various blends of the analytical high-grade hydroxyapatite and thermally calcined poultry manure hydroxyapatite samples are safe compared to the normal control rats with regards hepatic function and renal function parameters with the equal blend of analytical high grade and thermally calcined poultry manure-derived hydroxyapatite (1:1) possessing the lowest activity concentrations. In addition, the enzymatic (glutathione peroxidase) and non-enzymatic (reduced glutathione) antioxidant concentrations of the experimental animals administered the varied compositions of the analytical high grade and thermally calcined poultry manure-derived hydroxyapatite, were lower when compared to normal control rats. The microbiological evaluation suggests that the calcined poultry manure inclusion at various concentrations could not pose a negative effect on various pathology in the liver, kidney, and blood.

Anaerobic digestion of poultry manure to power a poultry farm in Ba: Pilot and techno-economic study.

Although poultry is the largest meat by volume produced in Fiji, there has not been any established study, nor application of the anaerobic digestion (AD) of poultry manure (PM) in the country. This paper aims to determine the techno-economic feasibility of the AD of PM to power a poultry farm in Fiji. A pilot scale study was first conducted with mono-digestion batches of poultry manure, and co-digestions with kitchen waste (KW) and newspaper waste (NPW). Solid state anaerobic digestion (SSAD) was employed in all the batches, and the key operational parameters of AD were studied, along with its influence on biogas production. The pilot study revealed that even slight changes in environmental temperature had the greatest effect on biogas production. The most resilient to the temperature changes were the co-digested feedstocks of KW. Yet, given a substantial AD period, the anaerobes in the mono-digesters were able to eventually acclimatize to the SSAD environment, and produce the overall highest biogas production. The pilot study results were then used to conduct a feasibility study of the full-scale design. The analysis showed that the SSAD system would generate 189.46 MWh of electricity annually, with a levelized cost of energy of FJ$0.17/KWh.

Mitigating water deficit stress in lemon balm (Melissa officinalis L.) through integrated soil amendments: A pathway to sustainable agriculture.

Lemon balm (Melissa officinalis L.) is a valuable medicinal plant, but its growth can be significantly impacted by drought stress. This study aimed to mitigate the adverse effects of water deficit stress on lemon balm biomass by integrating poultry manure compost, poultry manure biochar, NPK fertilizer, Trichoderma harzianum, Thiobacillus thioparus, and elemental sulfur as soil amendments. The experiment was conducted in a greenhouse using a completely randomized design with a factorial arrangement, consisting of three replicates. It included a water deficit stress factor at three levels (95-100%, 75-80%, and 55-60% of field capacity) and a soil amendment treatment factor with eleven different fertilizer levels. Treatments included control (no amendment), NPK fertilizer, poultry manure compost, poultry manure biochar, and combinations of these with T. harzianum, T. thioparus, and elemental sulfur under various water deficit levels. Water deficit stress significantly reduced photosynthetic pigments, gas exchange parameters, chlorophyll fluorescence, relative water content, and antioxidant enzyme activity, while increasing membrane permeability and lipid peroxidation in lemon balm plants. However, the integrated application of organic, biological, and chemical amendments mitigated these negative impacts. The combined treatment of poultry manure compost, poultry manure biochar, NPK fertilizer, T. harzianum, T. thioparus, and elemental sulfur was the most effective in improving the morpho-physiological properties (1.97-60%) and biomass (2.31-2.76 times) of lemon balm under water deficit stress. The results demonstrate the potential of this holistic approach to enhance the resilience of lemon balm cultivation in water-scarce environments. The integration of organic, biological, and chemical amendments can contribute to sustainable agricultural practices by improving plant morphological and physiological properties and plant performance under drought conditions.

Firmicutes primarily drive odor emission profiles in poultry manure treatments.

Odor emission during livestock manure treatment poses a threat to the environment and human health. However, the odor emission profiles and related factors of commonly employed poultry manure treatments have rarely been studied. Here, we explored the odor emission profiles of 3 common poultry manure treatments in China, namely, ectopic fermentation beds (EFB), annular composting troughs (ACT) and air-drying rooms (ADR). The results revealed that the total odor concentrations in the EFB, ACT and ADR groups were 2407.67 ± 512.94, 13444.00 ± 1269.92 and 621.33 ± 59.27, respectively. The ACT had the greatest number of odorants (31), followed by the ADR (27) and the EFB (24). Methyl mercaptan, acetic acid, acetaldehyde, hydrogen sulfide, ammonia and acrolein were the key odorous compounds detected in all the treatments. ACT contained the greatest number of key odorants (11) and exhibited an extensive co-occurrence relationship with the bacterial community. The 3 poultry manure treatments exhibited significant differences in the beta diversities of the bacterial community. The phylum of most bacteria associated with key odorants was Firmicutes, and Enterococcus and Oceanobacillus were significantly positively correlated with methyl mercaptan. The bacterial functional groups were enriched in carbohydrate metabolism, amino acid metabolism and energy metabolism, and the functional genes shaped the odor emission patterns in the poultry manure treatments. Redundancy analysis demonstrated that odor emission in the 3 treatments was positively correlated with Firmicutes abundance, pH, electrical conductivity and moisture. Thus, our study provides a good understanding of odor emission profiles in poultry manure treatments and data for precise odor emission control during livestock production.

Innovative insights into organic nitrogen degradation through protein family domains analysis in chicken and pig manure composting using metagenomic sequencing.

The nitrogen loss in composting is primarily driven by the transformation of organic nitrogen, yet the mechanisms underlying the degradation process remain incompletely understood. This study employed protein family domains (Pfams) analysis based on metagenomic sequencing to investigate the functional characteristics, key microorganisms, and environmental parameters influencing organic nitrogen degradation in chicken manure and pig manure composting. 154 Pfams associated with ammonification function were identified. Predominant Pfams: proteolytic peptidase, followed by chitin/cell wall degraders, least involved in nucleic acid degradation. Ammonifying microbial diversity was basically consistent among compost types, particularly in the thermophilic stage with the peak of abundance of dominant ammonifying microorganisms. Viruses played an important role in ammonification process, especially Uroviricota. 26 key ammonifying genera were identified by the microbial network. pH dominated the metabolic activity of ammonifying microorganisms in various manure compost types, primarily consisting of protein-degrading bacteria with stable community structures.

Nutrient release pattern and mitigation of N2O emissions under the application of activated poultry manure compost biochar with organic resources.

Biochar was generally used to reduce the macronutrient releases and to mitigate N2O gas emissions in cropland. This experiment evaluated the trend of major plant nutrient releases using the modified Hyperbola model and the greenhouse gas emissions by incorporating different poultry manure compost biochar with organic resources. The treatments consisted of the control as the organic fertilizer materials, the incorporated poultry manure compost biochar with organic fertilizer materials (PMCBF), and the incorporated plasma-activated poultry manure compost biochar with organic fertilizer materials (PAMBF) under redox conditions. The results showed that the cumulated highest concentrations of NH4-N and NO3-N were 2168.6 mg L-1 and 21.7 mg L-1 in the control, respectively. Compared with the control, the predicted reduction rates of NH4-N release from the PMCBF and PAMBF were 26.2% and 15.4%, respectively. In the control, the cumulated highest concentrations of PO4-P and K in leachate were 681.04 mg L-1 and 120.5 mg L-1, respectively. The predicted reduction rates of PO4-P and K were 55.1% and 15.5%, respectively, under the PAMBF compared to the control. The modified Hyperbola model with cumulated NH4-N, PO4-P, and K-releases under the treatments was a good fit (p < 0.0001). For greenhouse gas (GHG) emissions, the lowest cumulative N2O was 59.59 mg m-2 in the soil incorporated with PMCBF, and its reduction rate was 23.5% compared with the control. The findings of this study will contribute to more profound insights into the potential application of PAMBF and PMCBF as bio-fertilizers adapted to mitigate NH4-N, PO4-P, and K releases and N2O emissions, offering scientific evidence for organic farming strategies.

Effect of pyrolysis temperature on the binding characteristics of DOM derived from livestock manure biochar with Cu(II).

Biochar-derived dissolved organic matter (BDOM) has the potential to influence the environmental application of biochar and the behavior of heavy metals. In this study, the binding properties of BDOM derived from livestock manure biochar at different pyrolysis temperatures with Cu(II) were investigated based on a multi-analytical approach. The results showed that the DOC concentration, aromatics, and humification degree of BDOM were higher in the process of low pyrolysis of biochar. The pyrolysis temperature changed the composition of BDOM functional groups, which affected the binding mechanism of BDOM-Cu(II). Briefly, humic-like and protein-like substances dominated BDOM-Cu(II) binding at low and high pyrolysis temperatures, respectively. The higher binding capacity for Cu(II) was exhibited by BDOM derived from the lower pyrolysis temperature, due to the carboxyl as the main binding site in humic acid had high content and binding ability at low-temperature. The amide in proteins only participated in the BDOM-Cu(II) binding at high pyrolysis temperature, and polysaccharides also played an important role in the binding process. Moreover, the biochar underwent the secondary reaction at certain high temperatures, which led to condensation reaction of the aromatic structure and the conversion of large molecules into small molecules, affecting the BDOM-Cu(II) binding sites.

Neopusillimonas aromaticivorans sp. nov. isolated from poultry manure.

A polyphasic taxonomic approach was used to characterize a novel bacterium, designated strain CC-YST667T, isolated from poultry manure sampled in Taiwan. The cells were observed to be aerobic, motile and non-spore-forming rods, displaying positive reactions for oxidase. Optimal growth of CC-YST667T was observed at 25 °C, pH 8.0 and with 1 % (w/v) NaCl. The polar lipid profile consisted of phosphatidylmonomethylethanolamine, phosphatidylglycerol, diphosphatidylglycerol, phosphatidylethanolamine and multiple unidentified polar lipids. The major polyamine was spermidine. The major cellular fatty acids (>5 %) included C16 : 0, C17 : 0cyclo, C19 : 0cyclo ω8c and C14 : 0 3OH/iso-C16 : 1 I. On the basis of the results of analysis of 16S rRNA gene sequences, this isolate showed the closest phylogenetic relationship with 'Neopusillimonas minor' (with 98.2 % similarity) and Paralcaligenes ureilyticus (with 97.3 % similarity) of the family Alcaligenaceae. The draft genome, (3.3 Mb) with a DNA G+C content of 57.2 mol%, harboured various genes involved in the biodegradation of aromatic hydrocarbons. CC-YST667T shared highest orthologous average nucleotide identity (OrthoANI) with the type strains of species of of the genera Neopusillimonas (72.4‒77.9 %, n=2), Pusillimonas (72.8‒73.0 %, n=2) and Pollutimonas (71.7‒73.0 %, n=5). On the basis of its distinct phylogenetic, phenotypic and chemotaxonomic traits together with the results of comparative 16S rRNA gene sequencing, OrthoANI, digital DNA-DNA hybridization (DDH) and the phylogenomic placement, strain CC-YST667T is considered to represent a novel species of the genus Neopusillimonas, for which the name Neopusillimonas aromaticivorans sp. nov. is proposed. The type strain is CC-YST667T (=BCRC 81321T =JCM 34761T).

Use of coffee husks - comparison of pellet bedding quality, performance features, and some welfare indicators of broiler chickens.

BACKGROUND: The study aimed to evaluate the influence of wheat straw and different coffee husk (CHs) levels in pellet bedding on its quality, broiler chickens' performance, meat quality, and welfare indicators. In total, 200 Ross 308 chickens were divided into 4 groups: C - control with wheat straw pellet; CH10 - pellet with 10% CHs, CH25 - pellet with 25% CHs, and CH50 - pellet with 50% CHs. During 42 days of rearing, each bedding's physicochemical features were analyzed. The production results were controlled, and the footpad dermatitis, hock burns, and feather quality were assessed. From chosen birds, carcass composition was analyzed, as well as the qualitative features (color, water-holding capacity, drip loss) and breaking bone strength. RESULTS: The bedding material and rearing days influenced the content of dry matter, crude fiber, nitrogen, phosphorus, potassium, NDF, ADF, and pH. The results were inconclusive. The increasing trends in nitrogen, phosphorus, and potassium content were noticed at the end of rearing. Strong coefficient determination in bedding features was found (0.580 - 0.986). The pellet with CHs had no adverse effect on the growth performance of broilers. In the CH50 group, a lower fat percentage was found. A beneficial effect on water-holding capacity was noticed in leg muscles from CH10 and pectoral muscles from CH25. A significant decrease was found in footpad dermatitis incidence in groups CH25 and CH50. CONCLUSIONS: It can be concluded that CHs reuse in broilers as the pellet bedding material is possible due to the beneficial effect on some meat quality features and no adverse effect on the performance of broiler chickens. The positive impact on lower foot pad dermatitis incidence indicated the possibility of using CHs in pellet bedding.

Properties of Poultry-Manure-Derived Biochar for Peat Substitution in Growing Media.

Peat is considered a contentious input in horticulture. Therefore, there is a search for suitable alternatives with similar properties that can be used for partial or complete peat substitution in growing media. Poultry-manure-derived biochar (PMB) is considered such an alternative. This study aimed at determining the properties of PMBs obtained through pyrolysis at selected temperatures and assessing their potentials to substitute peat in growing media based on the selected properties. The scope included the laboratory-scale pyrolysis of poultry manure at the temperatures of 425-725 °C; the determination of selected physico-chemical and physical properties of the obtained biochars, including the contaminants; and the assessment of the potentials of produced biochars to be used as peat substitutes. PMBs contained less than 36% of total organic carbon (TOC). The contents of P and K were about 2.03-3.91% and 2.74-5.13%, respectively. PMBs did not retain N. They can be safely used as the concentrations of heavy metals, polycyclic aromatic hydrocarbons (PAHs), polychlorinatd biphenyls (PCBs), dioxins, and furans are within the permissible values (except for Cr). Due to high pH (9.24-12.35), they can have a liming effect. High water holding capacity (WHC) in the range of 158-232% w/w could allow for the maintenance of moisture in the growing media. PMBs obtained at 525 °C, 625 °C, and 725 °C showed required stability (H/Corg < 0.7).

The Influence of Poultry Manure-Derived Biochar and Compost on Soil Properties and Plant Biomass Growth.

Promising methods for managing poultry manure (PM) include converting poultry manure through pyrolysis to biochar, which can be used for soil applications. The overall goal of this study was to determine the effects of poultry manure-derived biochar and compost on the soil and growth of cherry tomatoes. The biochar obtained at 475 °C was characterized by a relatively high organic matter content of 39.47% and nitrogen content of 3.73%, while it had the lowest C/N ratio of 8.18. According to the recommendations of the EBC, the biochar obtained at 475 °C demonstrated the most beneficial effects in terms of fertilizing potential. The composting of poultry manure with the straw was successful, and the limit of 60 °C was exceeded, which allowed for the hygienization of the compost. The produced compost and biochar are sanitary safe and do not exceed the limits of heavy metal content. The lowest plant biomass was obtained from growing medium A with 3.6 g wet weight (0.24 g dry weight). The measurements of the height of cherry tomatoes showed that growing media D, E, and F allowed the plants to obtain from 602 to 654 mm in height.

[Distribution Characteristics and Risk Assessment of Tetracycline Antibiotics (TCs) in Soil-Vegetable System with Soil Fertilized with Animal Manure].

The effects of antibiotic contamination on vegetable safety and the ecological risks of soil after returning livestock and poultry manure to the land require sufficient future attention. Tetracycline antibiotics (TCs) are often detected at high concentrations in livestock manure and vegetable production soils. Recently, pot experiments and field investigation methods have often been used to understand the effects of TCs contamination on the vegetable safety and ecological risks of soil, whereas field experiments are employed less frequently. This study investigated the distribution characteristics of TCs in the soil-vegetable system following manure application using a combination of pot and field experiments. The human health risks of the edible parts of Chinese flowing cabbage were assessed using the health risk quotient method based on the acceptable daily intake (ADI-HQ), and the ecological risks of TCs-contaminated soils were evaluated using the risk quotient method associated with the species sensitivity distribution model (SSD-RQ). The results showed that oxytetracycline (OTC) was the major type of TCs in Chinese flowering cabbage based on both the pot and field experiments. The maximum contents (dry weight) of OTC in the aboveground parts of the Chinese flowering cabbage for the pot and field experiments were 29.25 µg·kg-1 and 45.03 µg·kg-1, respectively, whereas those of their underground parts were 87.32 µg·kg-1 and 135.44 µg·kg-1, respectively. Meanwhile, higher contents of TCs were detected in Chinese flowering cabbage collected from the field experiment than those from the pot experiment. OTC was also the major type of TCs in soil from both the pot and field experiments, with their contents up to 604.30 µg·kg-1 and 1013.68 µg·kg-1, respectively. Higher residual contents of three TCs were detected in soils collected from the field experiment than those from the pot experiment. Under the experimental conditions, with the except that OTC in Chinese flowering cabbage from the field experiment would pose medium health risks (HQ>0.1) to children, the contents of three TCs in other treated Chinese flowering cabbage would pose low health risks (HQ ≤ 0.1) to adults and children. In the pot experiments, three TCs present in Chinese flowering cabbage would pose low health risks (HQ ≤ 0.1) to adults and children. Additionally, the TCs in soils with manure application from the pot and field experiments may have posed both moderate or high levels of ecological risks (HQ>0.1 or HQ>1). Therefore, the effects of antibiotic contamination on vegetable safety and their potential ecological risks on soil following manure fertilization need to be given special attention.

On effect of poultry manure treatment with Effective Microorganisms with or without zeolite.

The decomposition process of poultry manure is generally mediated by microorganisms, whose degradation activity has beneficial effects on soil fertility but, on the other hand, leads to the generation of malodour gas. Indeed, a relevant problem of poultry farms is represented by the release of bad smells, which are mainly a consequence of decomposition process of chicken feces, chicken bedding, plumes, dropped feed, and dust. Furthermore, the unpleasant odour, associated with poultry manure degradation, not only limits its use in agriculture but also negatively affects the housing communities located near the farms. This study aimed at evaluating the effects in vitro of different doses of Effective Microorganisms (EM), mainly consisting of live communities of lactic acid bacteria, photosynthetic bacteria, and yeasts, on poultry manure alone or with zeolite, a porous mineral with absorbent and ion-exchange properties, belonging to the family of aluminosilicates. The obtained results demonstrated that these treatments were able to reduce the poultry manure malodours, associated mainly with a decrease in the ammonia (NH3) levels with respect to controls. The pH tended to increase, the nitrogen to go down, and the phosphorus to go up. Thus, all the effects described above were evident, testifying to a slower degradation of proteins, both with EM alone or in combination with zeolite. The presence of a pool of pesticides (65 components) was evaluated, and no variation was observed in the different experimental conditions versus control, as well as for REEs and metals. In conclusion, these preliminary results demonstrated that the use of EM with or without the addition of zeolite is a valid tool to eliminate the bad smell of manure and to make it a useful product as a fertilizer.

Post-hydrolysis versus side-stream ammonia stripping in semi-continuous two-stage anaerobic digestion of high nitrogen feedstock.

With the increased popularity of two-stage anaerobic digesters, post-hydrolysis ammonia stripping (PHAS) has resurfaced as a new possible treatment configuration. This study discusses for the first time the semi-continuous operation of PHAS and compares it with the most used stripping configuration nowadays, side-stream ammonia stripping (SSAS), under similar conditions using air or renewable natural gas (RNG) as stripping mediums. Ammonia stripping operating conditions were set to pH 9.5, 55 °C, and flowrate of 100 L gas/L/hour. RNG removed 50-58% of ammonia while air removed 70-78%. Interestingly, the PHAS system showed more flexibility and resilience than the SSAS system when testing parameters were changed. Volumetric and specific biogas production from PHAS and SSAS scenarios averaged up to 1.91 and 1.26 L/L/day and 831 and 701 L biogas/ kg VS/day under organic loading rates of 2.61 and 1.8 g VS/L/day, respectively.

Hydrothermal carbonization aqueous phase promotes nutrient retention and humic substance formation during aerobic composting of chicken manure.

The aqueous phase (AP) of hydrothermal carbonization is rich in humic substances (HSs), which could influence the poultry manure composting process and the product quality. Here, raw AP and its modified product (MAP) with different nitrogen (N) contents were added into chicken manure composting at low (5%) or high (10%) rate. Results showed that all APs addition decreased the temperature and pH but AP-10% increased total N, HSs, and humic acid (HA) of compost by 12%, 18% and 27%, respectively. MAP applications increased the total phosphorus by 8-9% and MAP-10% enhanced the total potussium content by 20%. Additionally, both AP and MAP additions increased the contents of three major components of dissolved organic matter by 20-64%. In conclusion, both AP and MAP can generally improve the chicken manure compost quality, which provides a new idea for the recycling of APs derived from agro-forestry wastes during hydrothermal carbonization.

Comparative effectiveness of exogenous organic amendments on soil fertility, growth, photosynthesis and heavy metal accumulation in cereal crops.

With soil fertility loss reached a critical state in arid and semi-arid regions, farmers are constrained to use mineral fertilizers, which are costly, non-eco-friendly and less effective in improving soil fertility than organic fertilizers such as dewatered sewage sludge (SS) and poultry manure (PM). In this regard, the current study aimed to highlight through experiments the positive effect of SS and PM applications on soil fertility and durum wheat growth. It targeted to demonstrate the safe and wise use of organic fertilization while assessing heavy metals in both soil and plant. The experiment was carried out in two batches of thirty-two pots, one for each treatment (SS and PM), in addition to the control with no fertilization. SS and PM were applied separately in three doses (D1 = 50 g, D2 = 100 g, and D3 = 200 g DM fertilizer/pot). The applications of both SS and PM induced a significant increase in plant-available phosphorus, organic matter, nitrates, moisture and electrical conductivity in soil, where these improvements were higher in PM compared to SS treatment. A significant accumulation of proline associated with an increase in biomass that were both proportional with fertilizer dose levels. Our findings revealed a loss in relative water content and leaf area of the plant. Correlations showed several significant relationships between soil parameters studied. The dose D2 of each fertilizer was the most efficient to improving both soil properties and plant components. Plant zinc concentration increased significantly with increase in soil zinc in PM amendments, however it decreased in SS. These relationships were not significant in copper for the two fertilizers. Both SS and PM improved soil fertility and plant growth compared to the control, thus this practice is a promising solution to tackle soil fertility loss and low production in drylands.

Evaluation of the methanogenic potential of anaerobic digestion of agro-industrial wastes.

Waste management technologies have become a way to generate value-added products. Anaerobic digestion (AD) allows biogas generation by treating organic wastes. In this work, the methanogenic potentials of anaerobic digestion of rumen and chicken manure, two typical agro-industrial wastes from the Colombian Caribbean region, were evaluated. On a first stage, the effect of temperature on anaerobic digestion of manure inoculated with liquid rumen was measured. Results revealed that the thermophilic digestion produces more biogas (up to 47% higher than the mesophilic digestion), but the mesophilic digestion has better biogas quality (up to 20% more methane than the thermophilic digestion). On the second experimental stage, it was assessed the effect of temperature regimen and the addition of fat-oil-grease (FOG) on cumulative biogas production, methane percentage, and physicochemical parameters. It was found that the anaerobic digestion of the rumen with FOG in mesophilic conditions had the best performance in terms of quantity and quality of biogas (2520 NL CH4/kg VS, CH4 93%, H2S 1 mg/L, H2O 16 mg/L). Finally, rumen and manure had methane concentrations above 40% in all cases studied, after 60 days of anaerobic digestion. It was concluded that rumen and manure are good candidates for biogas generation.

Impact of greywater on germination and physiological responses of Triticum aestivum L. HD 2967 in soil amended with poultry biochar.

ABSTRACTChemical fertilizers boost crop production; however, their continued use decreases soil fertility in the long run. Nutrient recycling by the beneficiation of poultry manure into biochar and application as a soil amendment is a long-term solution for plant nutrition. The effect of poultry manure, poultry biochar and crop irrigation with 50% and 100% greywater (GW) was assessed on soil properties and growth of wheat (Triticum aestivum L. HD-2967) on the 7th and 14th day of sowing. This resulted in greater nutrients (OC, OM, C:N have values of 3.51%, 6.58%, and 16.52, respectively) in soil on the 14th day after sowing irrigated with 100% GW for soil and 10 g biochar amendments than manure. The germination and growth were boosted to 100% on day 6 after seed sowing soil and 5 g biochar. The maximum number of leaflets (4), rootlets (7) and shoot length (26.58 cm) was obtained for soil and 10 g biochar amendments with 100% GW on the 14th day of sowing. The significance of the work is that greywater and poultry biochar has been used for the irrigation of wheat as a step towards the management of both solid and liquid waste. Biochar being a potential adsorbent reduces the pollutant load of greywater while increasing the nutritive value of soil substratum and influencing plant growth. The findings could offer crucial knowledge for creating agronomic procedures to repurpose the nutrients in poultry manure and biochar to grow crops by adding value to waste and meeting the goal of bioeconomic sustainability.

Biogas production from anaerobic co-digestion using kitchen waste and poultry manure as substrate-part 1: substrate ratio and effect of temperature.

The rapidly declining fossil fuels are no longer able to meet the ever-increasing energy demand. Moreover, they are considered responsible for greenhouse gas (GHG) emission, contributing to the global warming. On the other hand, organic wastes, such as kitchen waste (KW) and poultry manure (PM), represent considerable pollution threat to the environment, if not properly managed. Therefore, anaerobic co-digestion of KW and PM could be a sustainable way of producing clean and renewable energy in the form of biogas while minimizing environmental impact. In this study, the anaerobic co-digestion of KW with PM was studied to assess the rate of cumulative biogas (CBG) production and methane percentage in four digester setups (D1, D2, D3, and D4) operated in batch mode. Each digester setup consisted of five parallelly connected laboratory-scale digesters having a capacity of 1 L each. The digester setups were fed with KW and PM at ratios of 1:0 (D1), 1:1 (D2), 2:1 (D3), and 3:1 (D4) at a constant loading rate of 300 mg/L with 50 gm cow manure (CM) as inoculum and were studied at both room temperature (28 °C) and mesophilic temperature (37 °C) over 24 days. The co-digestion of KW with PM demonstrated a synergistic effect which was evidenced by a 16% and 74% increase in CBG production and methane content, respectively, in D2 over D1. The D3 with 66.7% KW and 33.3% PM produced the highest CBG and methane percentage (396 ± 8 mL and 36%) at room temperature. At mesophilic condition, all the digesters showed better performance, and the highest CBG (920 ± 11 mL) and methane content (48%) were observed in D3. The study suggests that co-digestion of KW and PM at mesophilic condition might be a promising way to increase the production of biogas with better methane composition by ensuring nutrient balance, buffering capacity, and stability of the digester.

The Use of Raw Poultry Waste as Soil Amendment Under Field Conditions Caused a Loss of Bacterial Genetic Diversity Together with an Increment of Eutrophic Risk and Phytotoxic Effects.

Poultry waste has been used as fertilizer to avoid soil degradation caused by the long-term application of chemical fertilizer. However, few studies have evaluated field conditions where livestock wastes have been used for extended periods of time. In this study, physicochemical parameters, metabarcoding of the 16S rRNA gene, and ecotoxicity indexes were used for the characterization of chicken manure and poultry litter to examine the effect of their application to agricultural soils for 10 years. Poultry wastes showed high concentrations of nutrients and increased electrical conductivity leading to phytotoxic effects on seeds. The bacterial communities were dominated by typical members of the gastrointestinal tract, noting the presence of pathogenic bacteria. Soils subjected to poultry manure applications showed statistically higher values of total and extractable phosphorous, increasing the risk of eutrophication. Moreover, while the soil bacterial community remained dominated by the ones related to the biogeochemical cycles of nutrients and plant growth promotion, losses of alpha diversity were observed on treated soils. Altogether, our work would contribute to understand the effects of common local agricultural practices and support the adoption of the waste treatment process in compliance with environmental sustainability guidelines.