Determination of α-methylenecyclopropylglycine in Shahi and China litchi cultivars at three different maturity stages: A quantitative study using liquid chromatography tandem mass spectrometry.

This study presents the contents of α-methylenecyclopropylglycine, a potentially toxic amino acid, in the peel, pulp and seed fractions of two well-known litchi varieties, namely Shahi and China, over a span of three harvest-seasons. For analysing α-methylenecyclopropylglycine, an LC-MS/MS-based method was validated. The method-accuracies fell within 75-110 % (RSD, <15 %) at 0.1 mg/kg (LOQ) and higher levels. A comparative evaluation of the results in peel, pulp and seed at 30 days before harvest (DBH), 15-DBH, and edible-ripe stage revealed that α-methylenecyclopropylglycine content increased as the litchi seeds grew towards maturity, regardless of the cultivar. In arils, at maturity, the concentration of α-methylenecyclopropylglycine ranged from not-detected to 11.7 µg/g dry weight. The Shahi cultivar showed slightly higher α-methylenecyclopropylglycine content in comparison to China litchi. This paper presents the first known analysis of combined seasonal data on different fruit components at various growth stages for the two chosen litchi cultivars grown in India.

Tapioca starch/konjac gum-based composite film incorporated with nanoliposomes encapsulated grape seed oil: Structure, functionality, controlled release and its preservation role for chilled mutton.

In this study, grape seed oil nanoliposomes (GSO-NLs) were constructed and doped into tapioca starch/konjac gum composite films (TK-GSO-NLs) to evaluate the preservation of chilled mutton. The results showed that the GSO-NLs have a good spherical or rounded state and good stability. The doping of GSO-NLs resulted in a smooth, flat, and dense structure on the surface and cross-section of the TK films. The TK-GSO-NLs showed the best compatibility among the components, with excellent mechanical and barrier properties. FTIR and XRD confirmed the presence of ionic bonds between the components, further improving the copolymer crystal structure. Notably, the packaging material provided ideal antioxidant and bacteriostatic stability as well as delayed GSO release. This packaging could effectively maintain the quality of chilled mutton and prolong the shelf-life to 15 days. The study provides ideas for the design of green and active food packaging and for extending the shelf life of meat.

Hyaluronic acid promotes heat-induced gelation of ginkgo seed proteins.

This study aimed to investigate how varying concentrations (0.01-0.5 %, w/v) and molecular weights (50, 500, 1500 kDa) of hyaluronic acid (HA) affect the physicochemical properties of heat-induced ginkgo seed protein isolate (GSPI)-HA composite gel. Incorporating HA increased viscosity (up to 14 times) and charge (up to 23 %) of GSPI-HA aggregates, while reducing particle size (up to 31 %) and improving gel texture, particularly with high molecular weight HA. However, high concentrations (0.5 %, w/v) of HA weakened gel texture. Non-covalent bonds primarily drive the formation of a continuous gel network between HA and GSPI, resulting in small pores and enhanced hydration properties. With increasing HA molecular weight, non-covalent interactions between GSPI and HA increased, leading to improved gel thermal stability. Overall, the study suggests that manipulating the molecular weight and concentration of HA can enhance the gelling properties of GSPI, leading to the development of a diverse array of GSPI-HA composite gels with varied properties.

Revival of cryopreserved larvae from the important aquaculture species Pacific White Shrimp (Litopenaeus vannamei) using vitrification and ultra-rapid laser warming.

Cryopreservation of aquatic embryos or larvae is needed to help safeguard genetics from important wild and captive species, increase aquaculture output, and meet the global demand for protein. To this end, the development of a cryopreservation protocol for nauplius larvae of the commercially important aquaculture species Litopenaeus vannamei, or Pacific White Shrimp, was pursued. Toxicity screening was performed using multiple cryoprotective agents (CPA), and a multi-constituent CPA cocktail was developed to achieve reliable vitrification of shrimp larvae encapsulated in 1.0-µL droplets containing gold nanoparticles. Vitrification and ultra-rapid laser warming were used to cryopreserve and revive nauplius-V stage larvae. Laser warming parameters were optimized to protect the pigmented eye spot from laser-induced ablation, and ice recrystallization inhibitors (IRIs) were tested to induce long-term survival. Approximately 54 % of revived larvae resumed active swimming, but all failed to molt to the zoea-I stage of development or live beyond 15 h post warming.

Microbial biosurfactant-mediated green synthesis of zinc oxide nanoparticles (ZnO NPs) and exploring their role in enhancing chickpea and rice seed germination.

Malnutrition is one of the greatest challenges faced by humanity, which may be addressed by improving crop productivity to ensure food security. However, extensive use of synthetic fertilizers can lead to soil fertility degradation. This study highlights the potential of combining nanotechnology with biotechnology to enhance the germination rates of commercially important crop seeds. Bacterial biosurfactant extracted from a newly isolated Klebsiella sp. strain RGUDBI03 was used as a reducing and capping agent for the synthesis of zinc oxide nanoparticles (ZnO NPs) through a simple method. Extensive characterization of ZnO NPs through electron microscopic analysis showed well-dispersed, homogeneous NPs with a size range of 2-10 nm. High-resolution transmission electron microscopy (HR-TEM) images also revealed molecular fringes of 0.26 nm in single crystal ZnO NPs, with approximately 50% of the NPs exhibiting a size range of 2-4 nm. X-ray diffraction (XRD) results of ZnO NPs indicated the presence of (100), (002), (101), (102), (200), and (112) planes, confirming their crystalline nature. The presence of C = C-H, C = C, C-H, and C = C groups in both the bacterial biosurfactant and ZnO NPs, as depicted by Fourier-transform infrared spectroscopy (FTIR) spectra, confirmed the function of the biosurfactant as a reducing and capping agent. The nano-primed chickpea (Cicer arietinum) and rice (Oryza sativa) seeds showed an increase in water uptake rate, 89% and 92% respectively, compared to the control (73% and 44%), leading to an enhanced germination rate of 98% and 76%, compared to their respective controls (80% and 30%) under optimized conditions. Additionally, the nano-primed seeds exhibited higher levels of α-amylase activity in both seeds (0.37 mg/g for chickpea and 2.49 mg/g for rice) compared to the control. Notably, the ZnO NP priming solution exhibited no cytotoxicity on red blood cells and earthworms (Eudrilus eugeniae), indicating their non-cytotoxic and eco-friendly nature for future field trials.

Inhibitory effects of phenylpropionamides from the hemp seed husk on tyrosinase.

Tyrosinase is an enzyme involved in melanin production. To identify inhibitors of this enzyme in natural products, extracts and fractions of hemp seed husk were screened for tyrosinase inhibition. Phenylpropionamides 1-9, which were isolated from the ethyl acetate fraction, had inhibitory effects; compounds 3 and 4 had the greatest inhibitory effects. Cannabisin A (3) was a non-competitive and cannabisin B (4) was a competitive inhibitor. The binding site and interaction on tyrosinase of each inhibitor were calculated by computational chemistry. Potential inhibitor 3 decreased the melanin content and tyrosinase activity in B16F10 melanoma cells. Therefore, cannabisin A (3) has potential as a tyrosinase inhibitor.

Applying integrated enhanced cognitive behaviour therapy (I-CBTE) to severe and longstanding eating disorders (SEED) Paper 2: An in-depth case study for clinicians.

BACKGROUND: This case study examines the application of Integrated Enhanced Cognitive Behavioural Therapy (I-CBTE) for a patient with severe, longstanding anorexia nervosa and multiple comorbidities, including organic hallucinosis, complex post-traumatic stress disorder (CPTSD), and severe self-harm. Such complex presentations often result in patients falling between services, which can lead to high chronicity and increased mortality risk. Commentaries from two additional patients who have recovered from severe and longstanding anorexia nervosa are included. CASE STUDY: The patient developed severe anorexia nervosa and hallucinosis after a traumatic brain injury in 2000. Despite numerous hospitalisations and various psychotropic medications in the UK and France, standard treatments were ineffective for 17 years. However, Integrated Enhanced Cognitive Behaviour Therapy (I-CBTE) using a whole-team approach and intensive, personalised psychological treatment alongside nutritional rehabilitation proved effective. METHODS: In this paper, we describe the application of the I-CBTE model for individuals with severe, longstanding, and complex anorexia nervosa, using lived experience perspectives from three patients to inform clinicians. We also outline the methodology for adapting the model to different presentations of the disorder. OUTCOMES: The patient achieved and maintained full remission from her eating disorder over the last 6 years, highlighting the benefit of the I-CBTE approach in patients with complex, longstanding eating disorder histories. Successful treatment also saved in excess of £360 k just by preventing further hospitalisations and not accounting for the improvement in her quality of life. This suggests that this method can improve outcomes and reduce healthcare costs. CONCLUSION: This case study, with commentaries from two patients with histories of severe and longstanding anorexia nervosa, provides a detailed description of the practical application of I-CBTE for patients with severe and longstanding eating disorders with complex comorbidities, and extensive treatment histories. This offers hope for patients and a framework for clinicians to enhance existing treatment frameworks, potentially transforming the trajectory of those traditionally deemed treatment resistant. RECOMMENDATIONS: We advocate the broader integration of CBT for EDs into specialist services across the care pathway to help improve outcomes for patients with complex eating disorders. Systematic training and supervision for multidisciplinary teams in this specialised therapeutic approach is recommended. Future research should investigate the long-term effectiveness of I-CBTE through longitudinal studies. Patient feedback on experiences of integrated models of care such as I-CBTE is also needed. In addition, systematic health economics studies should be conducted.

This case study, with commentaries from two other patients who have recovered from severe and longstanding anorexia nervosa, examines the use of Integrated Enhanced Cognitive Behavioural Therapy (I-CBTE) for a patient with severe and longstanding anorexia nervosa and multiple comorbidities. The patient had a history of multiple hospitalisations and was treated with various psychotropic medications without success for 17 years. However, she responded to I-CBTE. The model integrates multidisciplinary treatment to address the eating disorder and co-occurring conditions effectively. The patient achieved and maintained full remission from her eating disorder over the last 6 years, highlighting the effectiveness of the I-CBTE approach in patients with complex, longstanding and severe eating disorders. This intervention is cost-effective and has significant financial advantages for healthcare systems. The authors recommend further research into the long-term effectiveness of I-CBTE and broader integration of CBT for ED into clinical services and existing treatment frameworks to enhance care for patients with severe and longstanding eating disorders. Systematic training and supervision for multidisciplinary teams is needed and patient feedback on experiences of integrated models of care such as I-CBTE is also needed. Finally, systematic health economics studies should be conducted.

A pearl millet plasma membrane protein, PgPM19, facilitates seed germination through the negative regulation of abscisic acid-associated genes under salinity stress in Arabidopsis thaliana.

MAIN CONCLUSION: The pearl millet gene PgPM19 inhibits seed dormancy by negatively regulating the ABA biosynthesis and ABA signaling pathways in response to salinity stress in Arabidopsis. Abscisic acid (ABA) plays a pivotal role in orchestrating plant stress responses and development. However, how the ABA signal is transmitted in response to stresses remains primarily uncertain, particularly in monocotyledonous plants. In this study, PgPM19, a gene whose expression is induced by drought, salinity, heat, and ABA in both leaf and root tissues, was isolated from pearl millet. The expression of PgPM19 in yeast cells did not influence their growth when subjected to mannitol, sorbitol, or NaCl stress. However, Arabidopsis plants overexpressing PgPM19 (PgPM19_OE plants) exhibited increased germination rates, greater fresh weights and longer roots under salinity stress during germination, compared to wild-type (WT) plants. Conversely, the pm19L1 (SALK_075435) mutant, featuring a transfer DNA insertion in a closely related PgPM19 homolog (AT1G04560) in Arabidopsis, demonstrated reduced germination rates and smaller fresh weights under salinity-stressed condition than did WT and PgPM19_OE plants. A pivotal ABA biosynthesis gene, NCED3, ABA signaling pathway genes, such as PYL6 and SnRK2.7, alongside downstream ABI genes and stress-responsive genes RAB28 and RD29, were downregulated in PgPM19_OE plants, as evidenced by both transcriptome analysis and quantitative reverse transcription-PCR. These findings raise the possibility that PgPM19 is involved in regulating seed germination by mediating ABA biosynthesis and signaling pathway in response to salinity stress in Arabidopsis. This study contributes to a better understanding of PgPM19 in response to salinity stress and establishes a foundation for unraveling the crosstalk of stress responses and ABA in Arabidopsis and other plant species.

The effect of Coix lachrymal L. seed extract on the expression of inflammation and fibrogenesis genes in human retinal pigment epithelial cells.

Proliferative vitreoretinopathy (PVR) is a vision-threatening condition associated with retinal-detachment (RD), primarily caused by fibrocellular scar membrane formation. This study investigates the therapeutic potential of adlay seed extract fractions in mitigating PVR-associated pathways, focusing on oxidative stress, proliferation, inflammation, and fibrogenesis in retinal pigment epithelial (RPE) cells. Adlay seed extract fractions (methanolic: MeOH and residual: Res) were obtained through solvent extraction and characterized for carbohydrate, protein, flavonoid content, and antioxidant activity. RPE cells were cultured, and their viability in response to adlay fractions was assessed using the MTT assay. Gene expression analysis of IL-1ß, IL-6, LIF, TGF-ß, Snail and α-SMA genes was conducted via real-time PCR after treatment with adlay fractions. The Res fraction exhibited higher levels of protein, carbohydrate, flavonoids, and phenols compared to the MeOH fraction, along with significantly enhanced antioxidant activity. Both fractions showed inhibitory effects on RPE cell viability, with the Res fraction demonstrating a more pronounced impact. Gene expression analysis revealed a significant decrease in IL-6 and TGF-ß expression with the MeOH fraction treatment, while the Res fraction led to decreased expression of IL-6, LIF, TGF-ß, Snail and α-SMA, indicating a more comprehensive modulation of PVR-associated pathways. This study highlights the potential therapeutic benefits of adlay seed extract fractions in mitigating PVR-associated pathways in RPE cells. The Res fraction, particularly rich in bioactive compounds and exhibiting potent antioxidant activity, shows promise in attenuating oxidative stress, proliferation, inflammation, and fibrogenesis, critical processes in PVR development. These findings underscore the potential of adlay seed extracts as a novel therapeutic strategy for PVR warranting further investigation and clinical validation.

Effects of moisture regulation and heat treatment synergy on structural properties and digestibility of jackfruit seed starch.

To investigate the impact of moisture regulation and heat treatment synergy on the structural properties and digestibility of jackfruit seed starch (JSS), starch samples underwent heat-moisture treatment (HMT) and annealing treatment (ANN) with varying moisture content (10-30 % for HMT at 120 °C, and 50-90 % for ANN at 40 °C). The physicochemical properties and in vitro digestibility of modified-JSS were systematically investigated. Results showed that the birefringence intensity of HMT-JSS decreased at high moisture levels but remained unchanged for HMT-JSS and ANN-JSS at low moisture levels. As moisture content increased for HMT and ANN, the amylose content and relative crystallinity increased and then slightly decreased. The gelatinization temperatures increased while enthalpy and viscosity declined. At high moisture content, the infrared absorbance ratio of 1047 cm-1/1022 cm-1 decreased on HMT but increased on ANN. Resistant starch (RS) content of both HMT-JSS and ANN-JSS was increased at appropriate moisture levels (10-15 % for HMT, 50-80 % for ANN), but decreased with excessive moisture. Besides, these changes were more pronounced on HMT than ANN. Correlation analysis showed that the RS was significantly affected by the short-range ordered structure during HMT and ANN. These results revealed that hydrothermal treatment efficiently modified the structure properties and digestibility of JSS.

Biofortification of potato nutrition.

BACKGROUND: Potato (Solanum tuberosum L.) is the fourth most important food crop after rice, wheat and maize in the world with the potential to feed the world's population, and potato is a major staple food in many countries. Currently, potato is grown in more than 100 countries and is consumed by more than 1 billion people worldwide, and the global annual output exceeds 300 million tons. With the rapid increase in the global population, potato will play a key role in food supply. These aspects have driven scientists to genetically engineer potato for yield and nutrition improvement. AIM OF REVIEW: Potato is an excellent source of carbohydrates, rich in vitamins, phenols and minerals. At present, the nutritional fortification of potato has made remarkable progress, and the biomass and nutrient compositions of potato have been significantly improved through agronomic operation and genetic improvement. This review aims to summarize recent advances in the nutritional fortification of potato protein, lipid and vitamin, and provides new insights for future potato research. KEY SCIENTIFIC CONCEPTS OF REVIEW: This review comprehensively summarizes the biofortification of potato five nutrients from protein, lipid, starch, vitamin and mineral. Meanwhile, we also discuss the multilayered insights in the prospects of edible potato fruit, vaccines and high-value products synthesis and diploid potato seeds reproduction.

Hydroxyl radical-induced oxidation boosts the gelation of ginkgo seed protein in the presence of hyaluronic acid.

Hydroxyl radical-induced oxidation can modify gelling properties of food proteins. In this study, a hydroxyl radical generating system (HRGS), consisting of 0.1 mM Fe3+, 0.1 mM ascorbic acid, and 1, 10, or 20 mM H2O2, was used to oxidize ginkgo seed protein isolate (GSPI) for 4 h at ambient temperature in the presence of 0.3 % (w/v) hyaluronic acid (HA) to enhance its gelation properties. HRGS treatment led to increased protein hydrophobicity, reduced sulfhydryl content, and disulfide bond-mediated protein crosslinking. Moreover, the secondary structure of GSPI varied with H2O2 concentrations. Moderate oxidation (approximately 10 mM H2O2) promoted GSPI aggregation and improved mechanical strength, rheological properties, water holding capacity, and whiteness of GSPI gels. However, excessive oxidation disrupted hydrogen bonding, generated excessive disulfide bonds, hindered active group interaction, inhibited gel network formation, and reduced gel strength. Hence, hydroxyl radical-induced oxidation holds potential for enhancing GSPI gelation within specific concentration ranges. This study suggests that controlled oxidation could be a novel approach for developing protein-based gel products.

Effects of acetylated polysaccharide on the prebiotic properties of grape seed proanthocyanidins - Based on in vitro fermentation with Artemisia sphaerocephala Krasch glucomannan.

This study evaluated the impact of Artemisia sphaerocephala Krasch glucomannan with different degrees of acetyl group substitution (DS) on the prebiotic properties of grape seed proanthocyanidins (GSP). UV spectra, CIELab, and dynamic light scattering analyses indicated DS-influenced variable interactions between GSP and glucomannan. In vitro fermentation demonstrated that glucomannan enhanced the solubility of some phenolic compounds, and decreased the pH value of fermentation liquids. The production of acetate acid and total short chain fatty acids in the GSP fermentation liquid increased with the degree of DS of glucomannan. Notably, acetylated glucomannan exerted dramatic effects on GSP-induced gut microbiota modulation. The relative abundances of Bacteroides ovatus and Bacteroides decreased as DS increased. Meanwhile, Bacteroides acidifaciens and Akkermansia muciniphila have a positive correlation, even though the GSP-promoted enrichment of A. muciniphila was inhibited by the added glucomannan. Moreover, glucomannan enhanced the metabolism of nucleotides, secondary bile acid, and glycan inhibited by GSP. These findings suggest that acetylated glucomannan affect the prebiotic properties of GSP with its DS serving as a key factor.

Review and Call for Improved Cannabis Measurement and the Potential for Leveraging Cannabis Seed-To-Sale Systems.

International consensus has emerged, which proposes that the measurement and standardization of the quantity of THC potency, or dose (mgTHC), is a requisite for identifying consistent relationships between patterns of cannabis use and the risk for negative health outcomes, especially critical given the increasing number of U.S. states and European countries considering legalizing cannabis for recreational use. Despite the lack of measurement approaches that provide accurate, real-time data on THC dosage, the number of states that have implemented legal adult use cannabis markets has coincided with the use of regulatory seed-to-sale or track-and-trace systems. Seed-to-sale tracking systems are designed to track the amount of cannabis cultivated, and typically leverage biochemical testing contractors to label the THC potencies and amounts on a diverse array of cannabis products. We propose leveraging seed-to-sale tracking systems to facilitate a more objective measure of THC potency. In the current review, we will: 1) summarize the complexities of accurately measuring the multidimensional aspects of cannabis use patterns; 2) review recent advancements in quantifying THC and the importance of standardizing THC dose in measuring cannabis use; and 3) discuss the potential value of future research efforts examining how state-level seed-to-sale tracking data can inform the standard THC dose.

Effect of dietary supplementation of rubber seed kernel pellet on feed utilization, rumen fermentation, fatty acid profiles and health status in swamp buffalo.

Objective: Rubber seed kernel is a by-product of rubber cultivation and are high in oil and protein. This study was conducted to evaluate the effect of supplementing rubber seed kernel pellet (RUSKEP) on feed intake, nutrient digestibility, rumen fermentation, rumen fatty acid profiles, blood parameters, and immune response in swamp buffalo. Methods: Four male swamp buffalo with an initial body weight (BW) of 254 ± 10 kg and 26 months of age were used in this research. The experimental design was a 4  4 Latin squared design with RUSKEP supplementation at 0, 4, 6, and 8% of dry matter intake (DMI). Animals were fed concentrate at 1.0% BW, while rice straw was fed ad libitum. Results: Supplementation with RUSKEP did not change DMI or nutrient digestibility (p>0.05), while ether extract (EE) digestibility increased cubically with the addition of RUSKEP (p<0.01). The ruminal pH, ammonia-nitrogen (NH3-N), and the proportion of acetate (C2) were similar among treatments (p>0.05). The proportion of propionate (C3) increased linearly (p≤0.04), leading to a decrease in the acetate to propionate ratio (C2:C3) (p≤0.04) with the addition of RUSKEP. Furthermore, the butyrate (C4) proportion decreased linearly with RUSKEP supplementation (p=0.03). The addition of RUSKEP did not affect on linoleic acid (LA; C18:2 cis-9,12 + trans-9,12), or α-linolenic acid (ALA; C18:3 cis-9,12,15) (p>0.05). With RUSKEP supplementation, the stearic acid (C18:0) content increased quadratically (p<0.01). The increasing level of RUSKEP was higher in cholesterol and eosinophils (p≤0.03). The immune function (IgA, IgM, and IgG) was similar among treatments (p>0.05). Conclusion: Supplementing RUSKEP with up to 8% of DMI could improve rumen fermentation efficiency without affecting feed utilization, rumen PUFA profile, or immune response in swamp buffalo.

Effects of supplementing rumen-protected rubber seed oil to dairy cattle on feed digestibility and milk production.

Objective: The aim of this study was to determine the effect of rumen-protected rubber seed oil supplementation on feed digestibility, milk yield, and milk composition in tropical dairy cows. Methods: Twelve crossbred Holstien-Friesian dairy cows (75% Holstein-Friesian, 25% Thai native breed) with a mean body weight (BW) of 460 ± 30 kg and 20 ± 5 days in milk were randomly assigned to 1 of 3 treatments according to a completely randomized design. The treatments were as follows: a basal diet without rumen-protected fat (RPF) (control) or supplementation of rumen-protected palm oil (RPPO) at 300 g/h/d and rumen-protected rubber seed oil (RPRSO) at 300 g/h/d. Each cow was fed a total mixed ration ad libitum. Results: The nutrient intake was similar among treatments (p>0.05). Adding RPF did not affect nutrient digestibility, while organic matter digestibility increased in dairy cows receiving RPRSO (p<0.01). Blood urea nitrogen, total protein, or glucose did not alter among treatments (p>0.05), while triglycerides and cholesterol were increased when cows were fed RPPO (p<0.01). Adding RPF increases milk yield in cows (p<0.01). The supplementation of RPRSO increased milk fat (p=0.04). Milk fat yield was higher in RPPO and highest in RPRSO (p<0.01). The addition of RPF increased the oleic acid (OA, C18:1 cis-9) in milk (p=0.01). In addition, cows fed RPRSO increased linoleic acid (LA; C18:26 cis-9,12 + trans-9,12) and -linolenic acid (ALA; C18-33 cis-9,12,15) in milk (p<0.01). The addition of RPF increased milk unsaturated fatty acids (UFA) and monounsaturated fatty acids (MUFA) (P≤0.04). The PUFA in milk increased with RPRSO supplementation (p<0.01). Conclusion: Supplementation of RPRSO during early lactation can increase feed digestibility and the concentration of milk fat with PUFA (LA and ALA) in tropical dairy cows.

Seed priming enhances seed germination and plant growth in four neglected cultivars of Capsicum annuum L.

Priming is basically a water-based technique inducing controlled seed rehydration to trigger the metabolic processes normally activated during the early phase of germination. It is regarded as an ecofriendly approach alternative to fertilizers in traditional agriculture, but also a method to synchronize off-field crops and resume stored seeds, improving vigor, and allowing for a rapid, uniform seedling emergence. In this work we tested several methods of seed priming (i.e., hydro-priming, halopriming by KNO3, and acid priming with HCl) in four ancient and neglected cultivars of Capsicum annuum L., a crop species belonging to Solanaceae family cultivated worldwide. We followed germination performance, seedling growth and selected morphological traits, antioxidant production in the leaves, and protein content of the seeds. Apart from acid priming, which inhibited root emergence, both hydropriming and halopriming decreased the mean germination time in all cultivars. The best treatments were KNO3 6% for 96 h > KNO3 4% for 48 h > hydropriming for 24 h. In particular, KNO3 6% for 96 h in all four cultivars significantly increased plant growth, simple vigor index, development germination index, leaf antioxidant concentration and protein content in the seeds, in comparison to control and other priming treatments, indicating the prompt activation of pre-germinative processes.

A crude, cold-pressed oil from elderberry (Sambucus nigra L.) seeds: Comprehensive approach to properties and characterization using HPLC, DSC, and multispectroscopic methods.

The physicochemical characterization of fresh, undiluted, cold-pressed oil from elderberry seeds (EO) is presented. The results showed EO's uniqueness for the 93 % presence of essential fatty acids, including linoleic n-6 (41 %), α-linolenic n-3 (38 %), and oleic n-9 (13 %) acids with favorable ratios for human nutrition, n-3/n-6 = 0.93. A γ-tocopherol is the dominant tocopherol (96 %), with a concentration of 20.62 mg/100 g, indicating low oil oxidative stability. DSC heating and cooling traces determined the thermal properties. These results also revealed the presence of metastable triacylglycerol (TAG) structures composed of polyunsaturated fatty acids. The presence of characteristic groups for fatty acids and TAGs in EO was confirmed by FTIR-ATR spectra. For the first time, Langmuir monolayer studies on EO revealed its low compressibility, indicating its low emulsifiability, and the presence of minor components of EO, including tocopherols, phenolic acids, polyphenols, flavonoids, and carotenoids, was determined using UV-Vis absorption and fluorescence excitation-emission matrix (EEM) along with the chemometric method.

A seed-specific DNA-binding with One Finger transcription factor, RPBF, positively regulates galactinol synthase to maintain seed vigour in rice.

Seed vigour and longevity are intricate yet indispensable physiological traits for agricultural crops, as they play a crucial role in facilitating the successful emergence of seedlings and exert a substantial influence on crop productivity. Transcriptional regulation plays an important role in seed development, maturation, and desiccation tolerance, which are important attributes for seed vigour and longevity. Here, we have investigated the regulatory role of the seed-specific DNA binding with the One Finger (DOF) transcription factor and the RPBF (Rice P-box Binding Factor) in seed vigour. RPBF modulates the transcription of galactinol synthase and improves seed vigour. The promoter region of Galactinol synthase (GolS)-encoding genes from different species was enriched with DOF binding sites, and the expression levels of both RPBF and OsGolS were found to enhance during seed development. Furthermore, direct interaction of RPBF with OsGolS promoter has been demonstrated through multiple approaches: yeast one-hybrid (Y1H) assays, in planta promoter-GUS assays, dual luciferase assay, and in silico molecular docking. To assess functionality, Agrobacterium-mediated genetic transformation of rice was performed to generate the RNAi lines with reduced RPBF expression. In these RNAi lines, a reduction in both galactinol and raffinose content was observed. Since galactinol and raffinose are known contributors to seed vigour, the T2-transgenic lines were assessed for vigour and viability. For this, RNAi seeds were subjected to accelerated ageing by exposing them to high relative humidity and temperature, followed by scoring the germination and viability potential. Tetrazolium and seed germination assay revealed that the RNAi seeds were more sensitive to ageing compared to their wild-type and vector control counterparts. Collectively, this is the first report demonstrating that the DOF transcription factor RPBF controls the seed vigour through transcriptional regulation of galactinol synthase.

Yield, Physicochemical Properties, and Fatty Acid Profile of Vegetable Oil Extracted From Seed Coats of Strychnos pungens Soler. Fruits Collected in Chikomba, Zimbabwe.

The study examined Strychnos pungens Soler. (family LOGANIACEAE) fruit as a potential source of vegetable oil. Ripe fruits collected from a forested site in Zimbabwe were processed to determine the partitioning of fresh and dry fruit biomass. The oil was extracted from the seed coat using a hand-operated screw press, and its physiological properties were analyzed. Seeds contributed the most to the fresh weight of the fruit, followed by the shell and pulp. The seed coat was a significant component of the seeds. The seed coat, but not the pulp of the fruit, was found to contain screw press-extractable oil, the yield of which was substantial, amounting to around 39% of the dry weight of the seed coat. The oil was found to have a high free fatty acid content and a moderate iodine value (83 gI2/100 g), indicating a degree of unsaturation. Furthermore, the oil contained carotenoids and tocols, which serve as antioxidants that help to protect the oil from oxidation. The oil had a high content of monounsaturated oleic acid (78.3%), which is known for its stability and health benefits. The low levels of saturated and polyunsaturated fatty acids make it a high oleic oil. The volatile profile of the oil included compounds with pleasant fruity aromas that enhance its flavour and fragrance. The results highlighted the need for waste management strategies if S. pungens is industrialized as an oil crop. Significant waste, including shells, pulp, cake residue, and seed kernels, would need proper handling and valorisation. In summary, the research showed that S. pungens has the potential to be a valuable source of high-quality vegetable oil with good oxidative stability and health benefits, primarily due to its high content of oleic acid and antioxidant compounds.