Temporal effect of flaxseed oil in boar's diet on semen quality, antioxidant status and in-vivo fertility under hot humid sub-tropical condition.

The present study investigates the temporal effects of flaxseed supplementation on boar semen quality, antioxidant status, and in-vivo fertility under high-temperature humidity index (THI) conditions in a sub-tropical climate. Twelve Hampshire crossbreed boars were randomly assigned to control and treatment groups, with the treatment group receiving flaxseed oil supplementation. Semen samples were collected and analyzed for semen quality parameters, sperm kinematics, and antioxidant status. Fertility outcomes were assessed through in-vivo mating trials. Flaxseed supplementation resulted in time dependent significant improvements in semen volume, sperm concentration, total and progressive sperm motility, sperm quality parameters, and antioxidant status. Fertility outcomes, including farrowing rates and litter sizes, were also enhanced in the flaxseed-supplemented group. These findings highlight the potential of flaxseed supplementation to improve boar fertility under high ambient stress conditions, with implications for optimizing reproductive performance in swine production systems.

Unexpected arboviruses found in an epidemiological surveillance of acute tropical febrile syndrome in the department of Meta, Eastern Colombia.

BACKGROUND: Nonspecific acute tropical febrile illnesses (NEATFI) are common in the Latin American tropics. Dengue, Chikungunya, Zika, Mayaro, and Usutu, among others, can coexist in the American tropics. This study aimed to surveil the arboviruses that cause| acute febrile syndrome in patients in the Meta department, Colombia. METHODS: Between June 2021 and February 2023, an epidemiological surveillance study was conducted in the Llanos of the Meta department in Eastern Colombia. RESULTS: One hundred patients in the acute phase with typical prodromal symptoms of NEATFI infection who attended the emergency department of the Villavicencio Departmental Hospital were included. ELISA tests were performed for Dengue, Usutu, Chikungunya, and Mayaro. RT-qPCR was performed to detect the arboviruses Usutu, Dengue, Zika, Mayaro, and Oropouche. The seroprevalence for the Chikungunya, Mayaro, and Usutu viruses was 41 % (28/68), 40 % (27/67), and 62 % (47/75), respectively. Seroconversion for Chikungunya was observed in one patient; two seroconverted to Mayaro and one to Usutu. The NS5 gene fragment of the Usutu virus was detected in nine febrile patients. RT-qPCR of the remaining arboviruses was negative. The clinical symptoms of the nine Usutu-positive patients were very similar to those of Dengue, Chikungunya, Zika, and Mayaro infections. CONCLUSIONS: The pervasive detection of unexpected viruses such as Usutu and Mayaro demonstrated the importance of searching for other viruses different from Dengue. Because Usutu infection and Mayaro fever have clinical features like Dengue, a new algorithm should be proposed to improve the accuracy of acute tropical fevers.

Thermotolerance in Angus cattle is related to hair coat characteristics but not to coat color.

This study evaluated the impact of coat color (CC) and hair coat characteristics (HC) on productive and physiological traits related to thermotolerance in Angus heifers. The goal was to determine if HC and/or CC were reliable indicators of thermotolerance on a large scale for future breeding programs. Ninety-three 15-month-old Angus heifers (52 black, 41 red) were evaluated in three periods on a beef cattle farm in Brazil. Heifers were classified by CC and HC, and body weight, body condition score (BCS), and reproductive tract score (RTS) were compared between groups. In the summer evaluation, surface temperature (infrared thermography), internal temperature (intravaginal sensors), sweating rate, and behavior were assessed in a subset of heifers. Temperature-humidity index (THI) was calculated using meteorological data. The proportion of heifers with short, fine, and smooth hair (HC1) increased (P < 0.05) over the evaluations. Heifers with thick, long, and woolly hair (HC3) had lower (P < 0.05) body weights than those with finer coats, regardless of CC. Black heifers had greater (P < 0.05) puberty rates than red heifers in the first two evaluations. At a THI of 66, black heifers with HC1 exhibited a lower (P < 0.05) internal temperature compared to black heifers with HC3. At a THI of 75, all heifers with HC1 had lower (P < 0.05) internal temperatures, regardless of CC. Red heifers and those with HC3 experienced hyperthermia for longer (P < 0.05) periods. Neither HC nor CC affected (P > 0.05) surface temperatures or sweating rates. At a THI of 72, more black heifers remained standing, suggesting behavioral adaptation. In conclusion, coat color and characteristics influence thermal stress and performance in Angus heifers, though color impact is limited. Internal temperature monitoring effectively determines thermotolerance. In tropical regions, selecting for short, fine, smooth hair may improve heat tolerance.

Hematite nanomaterial from a tropical freshwater ecosystem: Geological, environmental, and industrial implications.

Synthetic hematite (Fe2O3) nanoparticles are extensively explored for medicine, optics, and environmental remediation. However, natural iron nanoparticles in a freshwater ecosystem have not been well characterized. Here we report the presence of natural iron nanoparticles in a tropical freshwater ecosystem in southern India. These iron nanoparticles that exist as slime in the natural water system were characterized through a multiproxy investigation involving Field-Emission Scanning Electron Microscopy (FE-SEM), X-ray Diffraction (XRD), X-ray Fluorescence (XRF), X-ray Photoelectron Spectroscopy (XPS), and Raman spectroscopy and BET analyses. These nanoparticles exist as amorphous hematite (Fe2O3), with the XRD peaks matching that of the iron arsenate compound. Fe2O3 occurs as mesoporous hollow microspheres with a size range of 14.97 to 61.3 nm and a surface area of 48.45m2/g. Further, the identification of Bacillus cereus in the slime suggests its role in iron sequestration, indicating a biogeochemical origin, which we infer is a particularly common phenomenon in tropical river basins where lateritic soils prevail. This study is the first to describe natural iron nanoparticles in a tropical freshwater ecosystem. It identifies their amorphous hematite structure and biogeochemical origin, offering new insights into their ecological roles and potential applications. This discovery presents an opportunity for utilizing this slime as an important source of hematite nanomaterials, with potential industrial applications.

Tunnel-Ventilated Sheds with Negative Pressure Reduce Thermal Stress and Improve the Meat Quality of Broilers.

This study aimed to evaluate the thermal performance and meat quality in broilers reared in positive pressure tunnel ventilation (PP) and negative pressure tunnel ventilation (NP) in production houses. 320 Cobb broilers (40 broilers per house) were used. Pectoralis major muscles from 40 broilers (10 broilers per house) were randomly selected and analysed for L* (lightness), a* (redness), b* (yellowness), pH, drip loss (DL), cooking loss (CL) and shear force (SF). Air temperature and humidity of the transportation and slaughterhouse waiting room were recorded in the last week of rearing. Subsequently, the enthalpy comfort index (ECI) was calculated. Air temperature and ECI were higher (p < 0.05) in positive pressure sheds, whereas relative humidity was higher (p < 0.001) in negative pressure sheds. There was no statistically significant difference between the enthalpy comfort index during transport and lairage (p > 0.005). Meat quality defects (high L*, DL, CL, SF) were found in PP and NP. It was observed that b* was higher in PP, although pH and CL were higher in NP. Differences in pH, b* and CL indicate that broilers from PP had a higher level of heat stress. In conclusion, differences in pH, b*value and cooking loss in breast broilers indicate that birds in PP had a higher level of heat stress. Additional studies investigating pre-slaughter handling methods to minimise injuries and heat stress are recommended in order to improve animal welfare and meat quality.

Probability and severity of climate change threats to natural world heritage sites vary across site specifics and over time.

This study contributes a first comparison of current and potential threats to Natural World Heritage Sites from climate change, as assessed by experts, when site and location characteristics (size, year of inscription to the World Heritage list, continent, climate zone and kind of site) are controlled for. The probability of a threat as well as its intensity is analysed. Another novelty lies in the use of data from the IUCN Conservation Outlook Assessment, covering all 245 Natural and Mixed World Heritage Sites across the world for three points in time: 2014, 2017 and 2020. The threat of climate change is broadly defined and includes temperature extremes, rising temperatures, disappearing glaciers, coral bleaching, droughts, desertification, and rising sea levels. Results based on a simultaneous Probit model with random effects show that the probability of actual and potential climate change threats increases over time, but with differences for size, kind of site and location. The probability that a threat is identified is highest for marine and coastal sites, and for those in Latin America, while it is significantly lower for sites on the African continent. Larger sites have a higher probability of being assessed as at risk and the severity of threats is found to be lower for recently inscribed sites. The rate at which the likelihood of a threat assessment increases is consistent for both current and future situations, while the probability of the most severe threat is larger for the current than the future period. A serious threat from climate change is assessed as highest for locations in the tropical monsoon (current period) or the tropical savannah climate (future period). Estimations also show that pure descriptive statistics or bivariate correlations may not correctly identify the risk or the dignity of a threat.

Behavioral Adaptations in Tropical Dairy Cows: Insights into Calving Day Predictions.

This study examined changes in the activity patterns of tropical dairy cows during the transition period to assess their potential for predicting calving days. This study used the AfiTag-II biosensor to monitor activity, rest time, rest per bout, and restlessness ratio in 298 prepartum and 347 postpartum Holstein Friesian cows across three lactation groups (1, 2, and ≥3). The data were analyzed using generalized linear mixed models in SPSS, and five machine learning models, including random forest, decision tree, gradient boosting, Naïve Bayes, and neural networks, were used to predict the calving day, with their performance evaluated via ROC curves and AUC metrics. For all lactations, activity levels peak on the calving day, followed by a gradual return to prepartum levels within two weeks. First-lactation cows displayed the shortest rest duration, with a prepartum rest time of 568.8 ± 5.4 (mean ± SE), which is significantly lower than higher-lactation animals. The random forest and gradient boosting displayed an effective performance, achieving AUCs of 85% and 83%, respectively. These results indicate that temporal changes in activity behavior have the potential to be a useful indicator for calving day prediction, particularly in tropical climates where seasonal variations can obscure traditional prepartum indicators.

Dynamics of dissolved inorganic carbon (DIC) and stable C isotope ratios (δ13CDIC) in a tropical watershed with diversified land use in São Paulo State, Brazil.

The fluvial transport of dissolved inorganic carbon (DIC) is an important component of the global carbon cycle. Herein, we assessed the dynamics of DIC and the C stable isotopic composition (δ13CDIC) in a watershed with diversified land use in São Paulo State (Brazil), more specifically in the Sorocaba River basin (SRB) and considered the temporal and spatial scales. For this purpose, twelve fluvial samples at each sampling point (e.g., S1, S2, S3, S4 and S5) were collected in the SRB, from June 2009 to May 2010, which represented one complete hydrological cycle that included the extremes of the rainfall and discharge regimes. In addition, the δ13CDIC values were also characterized in the wet and dry season at all sampling points to verify their seasonal variability. The results reflected the seasonal variation in discharges, water temperatures, and electrical conductivities. Most of the DIC was transported in the wet season at all sampling points, where the less negative δ13CDIC values were characterized. The natural sources of DIC are associated with atmospheric/soil CO2 consumption. The anthropogenic impacts on both [DIC] and δ13CDIC are linked to untreated urban sewage that is discharged directly into the Sorocaba River, as well as to aquatic photosynthesis in the Itupararanga Reservoir. From 1970 to 2020, the modeling proposed in this study indicated that the annual flux of DIC (Friver) varied from 9.0 to 78.7 t km-2 a-1, confirming that the El Niño Southern Oscillation (ENSO) controlled Friver in the SRB, with higher and lower Friver values occurring during strong El Niño (EN) and La Niña (LN) years. The average Friver value was 20 t km-2 a-1, which is higher than those obtained in natural several temperate and tropical watersheds due to the influences of urban areas on [DIC] in the SRB.

Assessment of the adaptive capacity of Morada Nova ewes with different coat coloration.

Coat color is a factor affecting heat tolerance in tropical ruminant and a particular coat color can determine which is more resilient to environmental changes. The aim of this study was to measure the level of adaptation of Morada Nova sheep with different coat color by using an Adaptability Index (AI). Adult ewes were used, including two different coat colors of Morada Nova sheep (red and white) with mean of body weight of 28.02 ± 5.70 kg and 31.47 ± 3.41 kg, respectively. Physiology parameters, hematology, electrolytes, acid-base status, mineral, renal functions, metabolites, enzymes, and proteins were measured. AI was designed using a multivariate approach (principal component analysis) to "weigh" the influence of each variable in the animal responses. The variables more important for adaptive aspects of Red Morada Nova were: haematology, electrolytes and acid-base status. The hemoglobin (HG), mean corpuscular hemoglobin (MCH), mean corpuscular hemoglobin concentration (MCHC), sodium (Na+), oxygen pressure (PO2), glucose (GLU) and albumin (ALB) were significantly higher in Red Morada Nova sheep and hydrogen carbonate (HCO3), base excess (BE), total carbon dioxide concentration (TCO2) and URE were significantly higher in the white phenotype. The variables more important for adaptive aspects of White Morada Nova sheep were: (K+), total protein (TP), PO2, HG, cholesterol (CHO), rectal temperature (RT) and glucose (GLU). Both phenotypes showed a high adaptation level, however, a higher value was generated for the Red Morada Nova sheep (81.97). This study concludes that both phenotypes of the Morada Nova sheep breed are well adapted to the climatic condition of the Brazilian tropical region using different adaptive mechanisms.

Remote sensing-based drought hazard monitoring and assessment in a coastal plain: A principal component approach.

Accurate drought information is essential for preventing agricultural and societal losses. The indicators of how severe a drought is the deficiency in precipitation, soil moisture, and vegetation stress. The indicators were evaluated using the Precipitation Condition Index (PCI), Vegetation Condition Index (VCI), and Temperature Condition Index (TCI).The indices were combined using Principal Component Analysis to create the Synthetic Drought Index (SDI) for the evaluation of drought severity. The indices were estimated using multi-source remote sensing data from the Tropical Rainfall Measuring Mission (TRMM) and Operational Land Imager (OLI) of various years. Temporal analysis showed that the district is drought-prone and deficiency of 65% of precipitation in northeast monsoon of 2016 and below average non-monsoon rainfall in 2017, caused drought and affected 223.5 Km2 in 2017. Below average precipitation in northeast monsoon of 2018 and below average non-monsoon rainfall in 2019, caused drought and affected 423 Km2 in 2019. The northeast coastal regions of Ottapidaram, Thoothukudi, and Vilathikulam taluks of the district were more severely prone to drought. Failure of monsoon is the root cause of water deficit in water bodies. The semi-arid coastal climate accelerates the evaporation of water in water bodies and causes soil moisture deficit that leads to drought in the coastal district. A sequential evaluation of this index can be used to identify the onset of drought and mitigate the effect of drought.

Sanya climatic-treatment cohort profile: objectives, design, and baseline characteristics.

Background: The prevalence of allergic diseases has increased globally, climate and environment also have important effects on respiratory or allergic diseases. However, population-based studies investigating the impact of tropical climates and environments on migratory-bird old people (MBOP) are lacking. Methods/Design: For this prospective cohort study, we recruited 756 participants from the community in Sanya City, Hainan Province, China. In addition to the completed baseline survey, a follow-up survey will be conducted during the periods of October-December and March-April for the next 3 years of MBEPs from northern China who spend the winter in Sanya. We will continue to record the height, weight, and blood pressure of all participants, as well as lung function for those with asthma and chronic obstructive pulmonary disease (COPD). Venous blood at baseline and urine samples will be collected during follow-up. Results: A total of 756 volunteers were recruited. Their average age is 66.1 years; 32.1% of them have high-school educations, while 37.3% have graduated from college or done undergraduate studies. The top five diseases in this cohort are allergic rhinitis (57.9%); eczema, urticaria, or dermatitis (35.6%); bronchitis and bronchiectasis (35.6%); asthma (14.7%); and emphysema (11.7%). Compared with their symptoms while at their summer places of residence, rates of remission reported by participants while living in Sanya were 80.4% for allergic rhinitis, 82.3% for bronchitis and emphysema, 85.2% for asthma, 96.0% for COPD (P < 0.001). Conclusions: The baseline survey has been completed. The preliminary findings support that a tropical climate may relieve the symptoms of allergic diseases in migratory-bird old people.

Data in experimental stand-alone microgrid: Solar production, domestic loads, battery storage and meteorological series.

At the centre of Reunion Island, a region denoted « Mafate ¼ is non-connected to the main electrical grid. In the context of the "Micro Reseau Mafate" research project, financed by the European Regional Development Fund, the PIMENT Laboratory and the local electrification union SIDELEC aim to develop microgrid stations in this isolated area. More than 300 homes are concerned by this alternative solution that these microgrids represent. Presented data come from an experimental microgrid between 3 homes at the place called « Roche Plate ¼, where electrical production is obtained by photovoltaic panels and storage by batteries. Local data are collected to simulate electrical systems and determine the optimal working point of energy distribution. These data concern the meteorological conditions, the electrical performance of devices and the inhabitants' usage of appliances. A weather station placed on the solar plant gives the meteorological conditions of this enclave region. Daily loads for the three families are measured and all the data are stored for analysis. A continuous period of one year of measurements is available for the community and is shared throughout this data paper. These datasets will be completed later. Electrical and meteorological data are collected in situ and stored in a central computer server located at the university.

Factors associated with allergic rhinitis and combined allergic rhinitis and asthma syndrome (CARAS) in adults undergoing immunotherapy in a tropical country: Is ISAAC-III questionnaire useful in adult populations?

BACKGROUND: Allergic diseases affect about 10-30% of the population in low- or middle-income tropical countries. Few studies describe the factors associated with allergic diseases in adult patients undergoing immunotherapy in Latin American countries. OBJECTIVE: This study aimed to determine the factors associated with allergic rhinitis (AR) and AR in comorbidity with asthma (CARAS) in adults treated with immunotherapy in two allergy referral centers in Bogotá (Colombia). MATERIAL AND METHODS: Observational, cross-sectional study conducted between January 2018 and January 2019. ISAAC-III and sociodemographic questionnaires were applied to determine the factors associated with AR and CARAS in adults treated with immunotherapy who attended the allergy consult at the Fundación Santa Fe de Bogotá and Unimeq-Orl. RESULTS: Among 416 adults aged 18-68 years, 71.4% (n = 297) were women. Regarding the sensitization results obtained by skin prick test, the most frequent allergens were house dust mites (64.18%): 49.03% were positive for both Dermatophagoides pteronyssinus and Dermatophagoides farinae, while 28.61% were positive for Blomia tropicalis. Excluding house dust mites, the most frequent allergens were dog hair (31.01%), cat hair (15.1%), grasses (15.9%), and food (15.9%). The main factor associated with exclusive AR was regular acetaminophen use more than four times a year: Prevalence ratio (PR) = 1.77 (95% CI: 1.12-2.25). The main factor associated with CARAS was cesarean delivery PR: 1.44 (95% CI: 1.09-1.78). CONCLUSION: The main factor associated with AR was regular acetaminophen use, while that associated with CARAS was cesarean delivery. The ISAAC-III questionnaire can be a useful low-cost tool to assess the factors associated with allergic diseases in adults in tropical countries.

The Horizontal Rain-Cell Span and Wind Impact on Multisite Diversity Scheme in a Tropical Region during El-Niño and La-Niña.

Site diversity is the most effective way to recover a signal lost during heavy downpours, especially in tropical regions since other mitigation techniques such as adaptive power control and code modulation may be unreliable during such. Duplicated links at diverse sites are deployed, and the least-attenuated signal of either site will be routed to the prime site for further operation. Since the deployment is costly, a diversity-gain model is used to estimate the appropriateness of selected sites. Diversity gain is known to depend on site-separation distance and elevation angle and, optionally, baseline angle and signal frequency, based on the region of research. In addition to these factors, the horizontal rain-cell span and the wind's impact on the gain are ongoing investigations, especially in tropical regions. This article presented the rain analysis from the year 2014 to mid-July 2017 at eight sites in the Gombak and Sepang districts of Malaysia to investigate the dependency relevancies. The rain rates were then used to predict the attenuation using the ITU-R P.618-13 rain-attenuation model, and the inter- and cross-district gain characteristics were evaluated. The observation of diurnal rain during the northeast seasons yielded that the northeast wind stimulates intense rain at locations along its direction, thus, extending the horizontal rain-cell span to 15 km distant from a host. Meanwhile, sites located at 5 km distant, slightly perpendicular to the wind direction, and from 90° to 180° from due north of the host, experience less rain. The baseline angle variation establishes nonimpact to the gain and lengthening the site-separation distance presented equal chances to the shorter span towards diversity-gain increment. The research outcome is necessary to formulate a more reliable diversity-gain model to be used in the industry.

Durability of Polymer-Modified Asphalt Mixture with Wasted Tire Powder and Epoxy Resin under Tropical Climate Curing Conditions.

The quality of pavements in tropical climates is negatively affected by the frequent wet and dry cycles during the rainy season, as well as by issues related to overloading from heavy trucks and traffic congestion. Contributing to this deterioration are factors such as acid rainwater, heavy traffic oils, and municipal debris. In light of these challenges, this study aims to assess the viability of a polymer-modified asphalt concrete mixture. This study investigates the feasibility of a polymer-modified asphalt concrete mixture with the addition of 6% crumb rubber powder from waste car tires and 3% epoxy resin to counter the harsh conditions of tropical climate weather. The study involved subjecting test specimens to five to 10 cycles of contaminated water (100% rainwater + 10% used oil from trucks), curing for 12 h, and air drying in a chamber of 50 °C for 12 h to simulate critical curing conditions. The specimens underwent fundamental laboratory performance tests such as the indirect tensile strength test, dynamic modulus test, four points bending test, and Cantabro test, as well as the double load condition in the Hamburg wheel tracking test to determine the effectiveness of the proposed polymer-modified material in actual conditions. The test results confirmed that the simulated curing cycles had a critical impact on the durability of the specimens, with the greater curing cycles leading to a significant drop in the strength of the material. For example, the TSR ratio of the control mixture dropped from 90% to 83% and 76% after five and 10 curing cycles, respectively. Meanwhile, the modified mixture showed a decrease from 93% to 88% and 85% under the same conditions. The test results revealed that the effectiveness of the modified mixture outperformed the conventional condition in all tests, with a more prominent impact observed under overload conditions. Under double conditions in the Hamburg wheel tracking test and a curing condition of 10 cycles, the maximum deformation of the control mixture sharply increased from 6.91 to 22.7 mm, whereas the modified mixture increased from 5.21 to 12.4 mm. Overall, the test results confirm the durability of the polymer-modified asphalt concrete mixture under harsh tropical climate conditions, promoting its application for sustainable pavements, especially in Southeast Asian countries.

NASA POWER satellite meteorological system is a good tool for obtaining estimates of the temperature-humidity index under Brazilian conditions compared to INMET weather stations data.

Heat stress negatively affects livestock, with undesirable effects on animals' production and reproduction. Temperature and humidity index (THI) is a climatic variable used worldwide to study the effect of heat stress on farm animals. Temperature and humidity data can be obtained in Brazil through the National Institute of Meteorology (INMET), but complete data may not be available due to temporary failures on weather stations. An alternative to obtaining meteorological data is the National Aeronautics and Space Administration Prediction of Worldwide Energy Resources (NASA POWER) satellite-based weather system. We aimed to compare THI estimates obtained from INMET weather stations and NASA POWER meteorological information sources using Pearson correlation and linear regression. After quality check, data from 489 INMET weather stations were used. The hourly, average daily and maximum daily THI were evaluated. We found greater correlations and better regression evaluation metrics when average daily THI values were considered, followed by maximum daily THI, and hourly THI. NASA POWER satellite-based weather system is a suitable tool for obtaining the average and maximum THI values using information collected from Brazil, showing high correlations with THI estimates from INMET and good regression evaluation metrics, and can assist studies that aim to analyze the impact of heat stress on livestock production in Brazil, providing additional data to complement the existing information available in the INMET database.

Energy characterization of residential and office buildings in a tropical location.

Governments have established regulations to reduce energy consumption and polluting gas emissions from buildings. In Colombia, the government issued Resolution 0549 in 2015, a regulation that imposed savings percentages on different types of buildings. Builders have had to improve their designs to meet this standard. However, this task requires a detailed understanding of the energy behaviour of buildings. In the absence of follow-up data, this study performed energy characterisations of a group of 20 residential and office buildings located in a tropical climate using DesignBuilder software. The simulations show a notable influence of plug-in loads on energy consumption as well as favourable thermal comfort conditions for all categories except for the low-income category. Solar radiation through windows is the largest source of heat in buildings. Additionally, the study shows the impact of a set of energy-saving measures on energy consumption. The results of this study can help designers reduce the energy consumption of tropical buildings and/or meet energy performance standards.

Comparative analysis of whole blood transcriptomics between European and local Caribbean pigs in response to feed restriction in a tropical climate.

BACKGROUND: Feed restriction occurs frequently during pig growth, either due to economic reasons or stressful environmental conditions. Local breeds are suggested to have better tolerance to periods of feed restriction. However, the mechanisms underlying the response to feed restriction in different breeds is largely unknown. The aims of the present study were (1) to compare the blood transcriptome profile in response to feed restriction and refeeding of two contrasted breeds, Large White (LW), which has been selected for high performance, and Creole (CR), which is adapted to tropical conditions, and (2) to investigate the effect of a moderate feed restriction and refeeding on whole blood transcriptome. Analysis of blood transcriptome allows to study the response to feed restriction and refeeding in a dynamic way. RNAseq was performed on blood samples of growing LW and CR pigs at two time points: after 3 weeks of feed restriction and after 3 weeks of refeeding. The data was compared with samples from control animals offered the same diet on an ad libitum basis throughout the whole experiment. RESULTS: In terms of performance (body weight and feed efficiency), CR pigs were less impacted by feed restriction than LW. The transcriptional response to feed restriction and refeeding between CR and LW was contrasted both in terms of number of DEGs and enriched pathways. CR demonstrated a stronger transcriptional response to feed restriction whereas LW had a stronger response to refeeding. Differences in the transcriptional response to feed restriction between CR and LW were related to cell stress response (Aldosterone Signalling, Protein ubiquitination, Unfolded Protein Signalling) whereas after refeeding, differences were linked to thermogenesis, metabolic pathways and cell proliferation (p38 MAPK, ERK/MAPK pathway). In both breeds, transcriptional changes related to the immune response were found after restriction and refeeding. CONCLUSIONS: Altogether, the present study indicates that blood transcriptomics can be a useful tool to study differential genetic response to feed restriction in a dynamic way. The results indicate a differential response of blood gene expression to feed restriction and refeeding between breeds, affecting biological pathways that are in accordance with performance and thermoregulatory results.

Incorporating theoretical and PRACTICAL approaches to assess the amount of sunlight CAPTURED by a tilted surface in a tropical climate.

Global solar radiation can theoretically be approximated in terms of tilt and azimuth of the surface regarding the impossibility of simultaneous measurement of solar radiation at various surface tilt and azimuth angles. Moreover, the random and anisotropic nature of diffuse radiation in a tropical climate makes it extremely difficult to estimate global solar radiation accurately as a function of surface tilt and azimuth angles. This study aims to develop a novel experimental and theoretical approach in the form of a computational network in order to determine a precise combined model integrated with global horizontal solar radiation to evaluate global tilted solar radiation in a tropical climate. Obtained results revealed that precisely estimation of the global tilted solar radiation was possible, by combining geometric factors for the tilted beam solar radiation, a combination of Gueymard and Louche models for the tilted diffuse solar radiation, and isotropic ground reflectance model for the ground reflected radiation, along with global horizontal solar radiation. It was observed that the accuracy of the model developed was higher for the partly sunny sky compared to the cloudy and rainy sky, estimates were more accurate on south-facing surfaces, and the model's accuracy declined with the increasing tilt angle of the surface. The statistical analysis exhibited excellent agreement between the measured data and simulation results, considering the value of normalized mean absolute error (nMAE %), normalized root mean squared error (nRMSE %), and mean absolute percentage error (MAPE %), which were in the ranges 0.22-0.94, 0.27-1.11, and 0.23-1.02, respectively for estimating global tilted solar radiation in various regions of Peninsular Malaysia, and they were respectively found in the range of 10.2-27.5%, 16.1-38.9%, and 6.0-17.8%, for evaluating the monthly optimum tilt angle towards the south, that leads to a loss of solar energy from 1.3 to 5.4 kWh/m2/year in Peninsular Malaysia. This search revealed that the experimental and theoretical approach employed in this study can be extended to more climatic regions.

Factors associated with allergic rhinitis and combined allergic rhinitis and asthma syndrome (CARAS) in children aged 5-18 years undergoing immunotherapy in a tropical low- or middle-income country.

BACKGROUND: Tropics have some particularities that can impact the natural history and factors associated with allergic diseases. However, few studies described the characteristics of patients with allergic rhinitis (AR) and combined AR and asthma syndrome (CARAS) in Latin American tropical countries. OBJECTIVE: This study aimed to determine the medical, social, and environmental factors associated with AR and CARAS in children aged 5-18 years treated with immunotherapy in two allergy referral centers in Bogotá (Colombia). MATERIAL: and methods: An observational, cross-sectional study was conducted between January 2018 and January 2019. International Study Asthma Allergies Childhood-III and sociodemographic questionnaires were applied to adolescents and parents of children undergoing immunotherapy at the allergy consult in the Hospital Universitario Fundación Santa Fe de Bogotá and UNIMEQ-ORL. RESULTS: Among 830 children aged 5-18 years, 38.1% (n = 316) were women. Up to 63.25% of the population had a positive skin prick test for house dust mites: 63.25% for Dermatophagoides pteronyssinus, 61.81% for Dermatophagoides farinae, and 31.57% for Blomia tropicalis. The factors associated with AR were male sex (PR: 1.31, 95% CI: 1.08-1.57), antibiotic consumption during the first year of life (PR: 0.80; 95% CI: 0.63-0.99), and exposure to dogs (PR: 1.32; 95% CI: 1.06-2.66). The factors associated with CARAS were older age (PR=0.95; 95% CI: 0.95-0.99), acetaminophen consumption over four times a year (PR: 1.31; 95% CI: 1.03-1.55), and antibiotic consumption during the first year of life (PR: 1.21; 95% CI: 1.06-1.34). CONCLUSION: A high prevalence of B. tropicalis was found in this study. The factors associated with AR and CARAS are like those described in high-income tropical countries. Further studies are needed in low- or middle-income tropical countries to identify modifiable factors associated with allergic diseases.