Eggshell derived scaffold of hydroxyapatite-ammonium bicarbonate nano-composite: Bioactivity and cytotoxicity studies.

This work investigated the facile synthesis of porous scaffold eggshell derived hydroxylapatite (ESHAp) as a composite with ammonium bicarbonate (AMB) for potential biomaterial in tissue engineering application. The phase purity, composition, size, functional groups and morphology of the apatite were elucidated using high resolution transmission electron microscopy (HTEM), X-Ray Diffraction (XRD), Fourier Transform Infrared Spectroscopy (FT-IR) and scanning electron microscopy (SEM). The results showed that hydroxylapatite (HAp) nanoparticles have round morphologies with average diameters between 20 nm and 80 nm, FT-IR analysis confirmed significant hydroxylapatite functional groups like carbonate, phosphate, and hydroxyl groups, while XRD analysis revealed a well crystalline monophasic HAp powder. The scaffold samples containing 10, 20, 25 and 30 % of AMB withstood a compressive stress up to 5, 20, 30 and 42 N/mm2 respectively which indicates that the compressive stress increased with the AMB content introduced as the pore forming agent. MTT assay performed using MG63 osteosarcoma cell lines showed that on comparing the sample of ESTHAp which contained 0 % AMB with other samples in the range of 0.01-1 mM, viability of above 85 % MG63 cells was achieved except for ESTHAp with 40 % AMB, which showed some level of toxicity. The cell adhesion studies of sintered ESTHAp porous scaffold with different weight percent of the pore forming agents using inverted microscopic images of MG 63 cells incubated with ESTHAp samples and treated with heat at 1000 °C appeared to be unstable in the media used with particle leaching observed, and no cells observed near to the samples.

Structure Formation in Engineered Wood Using Wood Waste and Biopolyurethane.

This research aims to find suitable processing methods that allow the reuse of wood waste to produce wood waste-based engineered wood logs for construction that meet the strength requirements for structural timber for sawn structural softwood. Three types of wood waste were examined: wood packaging waste (W), waste from the construction and furniture industry (PLY), and door manufacturing waste (DW). The wood waste was additionally crushed and sieved, and the granulometric composition and shape of the particles were evaluated. The microstructure of the surface of the wood waste particles was also analysed. A three-component biopolyurethane adhesive was used to bind wood waste particles. An analysis of the contact zones between the particles and biopolyurethane was performed, and the adhesion efficiency of their surfaces was evaluated. Analysis was performed using tensile tests, and the formation of contact zones was analysed with a scanning electron microscope. The wood particles were chemically treated with sodium carbonate, calcium hypochlorite, and peroxide to increase the efficiency of the contact zones between the particles and the biopolyurethane adhesive. Chemical treatment made fillers up to 30% lighter and changed the tensile strength depending on the solution used. The tensile strength of engineered wood prepared from W and treated with sodium carbonate increased from 8331 to 12,702 kPa compared to untreated waste. Additionally, the compressive strength of engineered wood made of untreated and treated wood waste particles was determined to evaluate the influence of the wood particles on the strength characteristics.

Effect of Compressive Stress on Copper Bonding Quality and Bonding Mechanisms in Advanced Packaging.

The thermal expansion behavior of Cu plays a critical role in the bonding mechanism of Cu/SiO2 hybrid joints. In this study, artificial voids, which were observed to evolve using a focused ion beam, were introduced at the bonded interfaces to investigate the influence of compressive stress on bonding quality and mechanisms at elevated temperatures of 250 °C and 300 °C. The evolution of interfacial voids serves as a key indicator for assessing bonding quality. We quantified the bonding fraction and void fraction to characterize the bonding interface and found a notable increase in the bonding fraction and a corresponding decrease in the void fraction with increasing compressive stress levels. This is primarily attributed to the Cu film exhibiting greater creep/elastic deformation under higher compressive stress conditions. Furthermore, these experimental findings are supported by the surface diffusion creep model. Therefore, our study confirms that compressive stress affects the Cu-Cu bonding interface, emphasizing the need to consider the depth of Cu joints during process design.

Investigating the Dynamic Mechanical Properties and Strengthening Mechanisms of Ti-6Al-4V Alloy by Using the Ultrasonic Surface Rolling Process.

The demand for titanium alloy has been increasing in various industries, including aerospace, marine, and biomedical fields, as they fulfilled the need for lightweight, high-strength, and corrosion-resistant material for modern manufacturing. However, titanium alloy has relatively low hardness, poor wear performance, and fatigue properties, which limits its popularization and application. These disadvantages could be efficiently overcome by surface strengthening technology, such as the ultrasonic surface rolling process (USRP). In this study, the true thermo-mechanical deformation behavior of Ti-6Al-4V was obtained by dynamic mechanical experiment using a Hopkinson pressure bar. Moreover, USRP was applied on the Ti-6Al-4V workpiece with different parameters of static forces to investigate the evolution in surface morphology, surface roughness, microstructure, hardness, residual stress, and fatigue performance. The strain rate and temperature during the USRP of Ti-6Al-4V under the corresponding conditions were about 3000 s-1 and 200 °C, respectively, which were derived from the numerical simulation. The correlation between the true thermo-mechanical behavior of Ti-6Al-4V alloy and the USRP parameters of the Ti-6Al-4V workpiece was established, which could provide a theoretical contribution to the optimization of the USRP parameters. After USRP, the cross-sectional hardness distribution of the workpiece was shown to initially rise, followed by a subsequent decrease, ultimately to matrix hardness. The cross-sectional residual compressive stress distribution of the workpiece showed a tendency to initially reduce, then increase, and finally decrease to zero. The fatigue performance of the workpiece was greatly enhanced after USRP due to the effect of grain refinement, work hardening, and beneficial residual compressive stress, thereby inhibiting the propagation of the fatigue crack. However, it could be noted that the excessive static force parameter of USRP could induce the decline in surface finish and compressive residual stress of the workpiece, which eliminated the beneficial effect of the USRP treatment. This indicated that the choice of the optimal USRP parameters was highly crucial. This work would be conducive to achieving high-efficiency and low-damage USRP machining, which could be used to effectively guide the development of high-end equipment manufacturing.

RhNi Bimetallenes with Lattice-Compressed Rh Skin towards Ultrastable Acidic Nitrate Electroreduction.

Harvesting recyclable ammonia (NH3) from acidic nitrate (NO3 -)-containing wastewater requires the utilization of corrosion-resistant electrocatalytic materials with high activity and selectivity towards acidic electrochemical nitrate reduction (NO3ER). Herein, ultrathin RhNi bimetallenes with Rh-skin-type structure (RhNi@Rh BMLs) are fabricated towards acidic NO3ER. The Rh-skin atoms on the surface of RhNi@Rh BMLs experience the lattice compression-induced strain effect, resulting in shortened Rh-Rh bond and downshifted d-band center. Experimental and theoretical calculation results corroborate that Rh-skin atoms can inhibit NO2*/NH2* adsorption-induced Rh dissolution, contributing to the exceptional electrocatalytic durability of RhNi@Rh BMLs (over 400 h) towards acidic NO3ER. RhNi@Rh BMLs also reveal an excellent catalytic performance, boasting a 98.4% NH3 Faradaic efficiency and a 13.4 mg h-1 mgcat -1 NH3 yield. Theoretical calculations reveal that compressive stress tunes the electronic structure of Rh skin atoms, which facilitates the reduction of NO* to NOH* in NO3ER. The practicality of RhNi@Rh BMLs has also been confirmed in an alkaline-acidic hybrid zinc-nitrate battery with a 1.39 V open circuit voltage and a 10.5 mW cm-2 power density. This work offers valuable insights into the nature of electrocatalyst deactivation behavior and guides the development of high-efficiency corrosion-resistant electrocatalysts for applications in energy and environment.

Bacteria Colonies Modify Their Shear and Compressive Mechanical Properties in Response to Different Growth Substrates.

Bacteria build multicellular communities termed biofilms, which are often encased in a self-secreted extracellular matrix that gives the community mechanical strength and protection against harsh chemicals. How bacteria assemble distinct multicellular structures in response to different environmental conditions remains incompletely understood. Here, we investigated the connection between bacteria colony mechanics and the colony growth substrate by measuring the oscillatory shear and compressive rheology of bacteria colonies grown on agar substrates. We found that bacteria colonies modify their own mechanical properties in response to shear and uniaxial compression in a manner that depends on the concentration of agar in their growth substrate. These findings highlight that mechanical interactions between bacteria and their microenvironments are an important element in bacteria colony development, which can aid in developing strategies to disrupt or reduce biofilm growth.

Spherical Cap Studs: A novel speed bump alternative to reduce discomfort with effective speed reduction.

OBJECTIVE: The aim of this study is to assess the potential of Spherical Cap Studs (SCS) as a substitute for conventional speed bumps, with a focus on limiting two wheeler speed while minimizing discomfort to riders by comparing the speed reduction capabilities and discomfort levels associated with SCS and speed bumps. METHODS: The study uses experimental approach to compare the speed limiting ability and discomfort caused to rider by the proposed SCS and a standard speed bump. Speed profiles were developed for two wheelers passing over both SCS and speed bump. The parameter employed to compare speed profiles is the Mean Absolute Percentage Difference (MAPD), offering valuable insights into how effectively the two traffic calming measures reduce two wheeler speeds. To compare discomfort, the study calculates the 'Vibration Dose Value' (VDV) experienced by riders when traversing both speed bump and SCS. Additionally, 'Static Compressive Stress' (Se) applied to the spinal cord is also calculated in both scenarios. RESULTS: The analysis of speed profiles reveals an MAPD value of 13.70% indicating that SCS exhibits speed reduction capabilities comparable to traditional speed bump. In terms of discomfort, the VDV for two wheelers passing over a speed bump is measured at 5.92 m/s1.75, whereas the VDV for SCS is found to be 5.16 m/s1.75. Similarly, the Static Compressive Stress (Se) experienced at a speed bump is 0.60 MPa, in contrast to the 0.33 MPa recorded for SCS. This data underscores a noteworthy 12.8% reduction in VDV and a substantial 45.57% reduction in Se. CONCLUSION: The study's findings support the potential adoption of SCS as an effective alternative to conventional speed bumps for controlling two wheeler speeds. SCS demonstrate a speed reduction capability similar to that of traditional speed bumps while significantly alleviating discomfort for riders. SCS is expected to be a promising solution for traffic calming purposes in various settings, such as markets, residential areas, institutional campuses, and parking lots.

Long-Term Oxidation of Zirconium Alloy in Simulated Nuclear Reactor Primary Coolant-Experiments and Modeling.

Oxidation of Zr-1%Nb fuel cladding alloy in simulated primary coolant of a pressurized water nuclear reactor is followed by in-situ electrochemical impedance spectroscopy. In-depth composition and thickness of the oxide are estimated by ex-situ analytical techniques. A kinetic model of the oxidation process featuring interfacial reactions of metal oxidation and water reduction, as well as electron and ion transport through the oxide governed by diffusion-migration, is parameterized by quantitative comparison to impedance data. The effects of compressive stress on diffusion and ionic space charge on migration of ionic point defects are introduced to rationalize the dependence of transport parameters on thickness (or oxidation time). The influence of ex-situ and in-situ hydrogen charging on kinetic and transport parameters is also studied.

Sapropel as a Binding Material for Wood Processing Waste in the Development of Thermal Insulation Biocomposite.

When developing new innovative building materials, their performance characteristics as well as their environmental friendliness are important. It is difficult to produce a fully ecological material for building envelopes, because there is a lack of ecological binding materials on the market, good binding materials are very expensive, and cheaper ones have poorer adhesive properties and performance characteristics. In this work, natural organic sapropel was used as an ecological binder. Before use, an organic sapropel was additionally mechanically activated. Its activation efficiency was evaluated on the basis of consistency and tensile strength. Sapropel activation increased its consistency from 112 to 168 mm and its tensile strength from 466 to 958 kPa. Wood processing waste was used as a filler for the thermal insulation biocomposite. Additionally, the wood waste was chopped to regulate the density and main performance properties of the biocomposite. The density of the biocomposite was also regulated using different amounts of sapropel and the degree of compaction of the composite mixture. In this work, the influence of the amount of sapropel, the level of compression of the biocomposite mixture, and the size of the wood waste particles on the thermal conductivity and compressive stress of the biocomposite was analyzed. It was found that the compression level had the greatest influence on both the compressive stress and thermal conductivity, up to 12 times and 43.3%, respectively.

Precision Hydrogels for the Study of Cancer Cell Mechanobiology.

Cancer progression is associated with extensive remodeling of the tumor microenvironment (TME), resulting in alterations of biochemical and biophysical cues that affect both cancer and stromal cells. In particular, the mechanical characteristics of the TME extracellular matrix undergo significant changes. Bioengineered polymer hydrogels can be instrumental to systematically explore how mechanically changed microenvironments impact cancer cell behavior, including proliferation, survival, drug resistance, and invasion. This article reviews studies that have explored the impact of different mechanical cues of the cells' 3D microenvironment on cancer cell behavior using hydrogel-based in vitro models. In particular, advanced engineering strategies are highlighted for tailored hydrogel matrices recapitulating the TME's micrometer- and sub-micrometer-scale architectural and mechanical features, while accounting for its intrinsically heterogenic and dynamic nature. It is anticipated that such precision hydrogel systems will further the understanding of cancer mechanobiology.

Compressive stress induces spinal vertebral growth plate chondrocytes apoptosis via Piezo1.

Many clinical studies have indicated an association between biomechanical factors and the incidence and pathological progression of adolescent idiopathic scoliosis (AIS). However, at present, the research on AIS is mainly focused on the etiology, and there are few studies reporting the causes of progressive aggravation of AIS. In the present study, we aim to investigate the role of Piezo1 in compressive stress-induced mouse spinal vertebral growth plate chondrocytes apoptosis. First, a scoliosis mouse model was established, and the expression of Piezo1 as well as the degree of apoptosis were investigated. We found that the expression of Piezo1 and the degree of apoptosis were significantly higher on the concave sides than that on the convex sides of the vertebral growth plate in mice with scoliosis. Spinal vertebral growth plate chondrocytes were further isolated and treated with Yoda1 to mimic Piezo1 overload. Excess Piezo1 significantly promoted apoptosis of spinal vertebral growth plate chondrocytes. Moreover, static gas compressive stress was used to simulate the increased concave compressive stress in the process of scoliosis with or without GsMTx4, a Piezo inhibitor. It was observed that with the increase of static compressive stress, the expression of Piezo1 increased, and the chondrocytes of vertebral growth plate treated with Piezo1 inhibitor GsMTx4 weakened the above phenomena. In conclusion, our results indicated that compressive stress is strongly associated with the different degrees of apoptosis on both sides on the convex and concave sides of the vertebral growth plate in scoliosis via inducing different expressions of Piezo1. Reducing the expression of Piezo1 in the concave side of the vertebral growth plate and inhibiting the apoptosis of chondrocytes in the bilateral vertebral growth plate caused by asymmetric stress on both sides of the concave vertebral body may be a promising treatment strategy for AIS.

Comparative Studies of c- and m-Plane AlN Seeds Grown by Physical Vapor Transport.

The ultra-wide bandgap semiconductor AlN has attracted a great deal of attention owing to its wide application potential in the field of electronics and optoelectronic devices. In this report, based on the mechanism of the physical vapor transport (PVT) growth of AlN crystal, the c- and m-plane AlN seed crystals were prepared simultaneously through special temperature field design. It is proved that AlN crystals with different orientations can be obtained at the same temperature field. The structure parameter of AlN crystal was obtained through the characteristic evaluations. In detail, XPS was used to analyze the chemical states and bonding states of the surface of seed crystals. The content of oxygen varied along with distinct orientations. Raman spectrum documented a small level of compressive stress on these crystal seeds. Tested results confirmed that the prepared AlN crystal seeds had high quality.

Low-Cost Fiber Chopped Strand Mat Composites for Compressive Stress and Strain Enhancement of Concrete Made with Brick Waste Aggregates.

Given the excessive demolition of structures each year, the issues related to the generated structural waste are striking. Bricks being a major constituent in the construction industry, also hold a significant proportion of the construction waste generated annually. The reuse of this brick waste in new constructions is an optimal solution considering cost-effectiveness and sustainability. However, the problems related to the substandard peak stress and ultimate strain of concrete constructed with recycled brick aggregates (CRAs) limit its use in non-structural applications. The present study intends to improve the unsatisfactory mechanical characteristics of CRAs by utilizing low-cost glass fiber chopped strand mat (FCSM) sheets. The efficacy of FCSM sheets was assessed by wrapping them around CRA specimens constructed with different concrete strengths. A remarkable increase in the peak compressive stress and the ultimate strain of the CRA specimens were observed. For low, medium, and high strength CRAs, the ultimate strain improved by up to 320%, 308%, and 294%, respectively, as compared to the respective control specimens. Several existing analytical models were utilized to predict the peak compressive stress and ultimate strain of the CRAs strengthened using FCSM sheets. None of the considered models reproduced experimental results accurately. Therefore, equations were formulated using regression predicting the peak stress and ultimate strain of the CRAs confined with FCSM sheets. The predicted values were found to correlate well with the experimental values.

The Prediction of Compressive Strength and Compressive Stress-Strain of Basalt Fiber Reinforced High-Performance Concrete Using Classical Programming and Logistic Map Algorithm.

In this research, the authors have developed an algorithm for predicting the compressive strength and compressive stress-strain curve of Basalt Fiber High-Performance Concrete (BFHPC), which is enhanced by a classical programming algorithm and Logistic Map. For this purpose, different percentages of basalt fiber from 0.6 to 1.8 are mixed with High-Performance Concrete with high-volume contact of cement, fine and coarse aggregate. Compressive strengths and compressive stress-strain curves are applied after 7-, 14-, and 28-day curing periods. To find the compressive strength and predict the compressive stress-strain curve, the Logistic Map algorithm was prepared through classical programming. The results of this study prove that the logistic map is able to predict the compressive strength and compressive stress-strain of BFHPC with high accuracy. In addition, various types of methods, such as Coefficient of Determination (R2), are applied to ensure the accuracy of the algorithm. For this purpose, the value of R2 was equal to 0.96, which showed that the algorithm is reliable for predicting compressive strength. Finally, it was concluded that The Logistic Map algorithm developed through classical programming could be used as an easy and reliable method to predict the compressive strength and compressive stress-strain of BFHPC.

Effect of compressive stress on cavitation erosion-corrosion behavior of nickel-aluminum bronze alloy.

The effect of compressive stress on cavitation erosion-corrosion behavior of nickel-aluminum bronze alloy was investigated, and the results showed that the alloy exhibited selective phase corrosion of eutectoid "α + κiii" and its destruction was aggravated with more cavitation mass loss up to 1.74 times of the specimen without stress. It was mainly owing to the enhanced corrosion-induced erosion caused by compressive stress, which led to lattice distortion of the alloy and the resulting accelerated selective phase corrosion with increasing surface roughness, and then intensified the synergistic effect of electrochemical corrosion and mechanical erosion.

Investigation on the Air Permeability and Pore Structure of Concrete Subjected to Carbonation under Compressive Stress.

Concrete structures have to withstand the combined effects of external load and environmental factors. Therefore, it is meaningful to study the durability of concrete under compression and carbonation. The air permeability coefficient (kAu) and pore structure of concrete under uniaxial compression and carbonation were measured by the Autoclam method and mercury intrusion porosimetry (MIP). The Autoclam test results showed that the concrete kAu changed in a concave parabolic manner with the compressive stress level, and the inflection point of the stress level was 45%. The MIP results showed that the characteristic pore structural parameters (porosity, average pore diameter, median pore diameter by area, and median pore diameter by volume) first decreased and then increased with the stress level change. The change in concrete microstructure was a result of the combined effect of pore filling, decalcification, and densification, as well as the split effect. The key pore structural parameters affecting kAu were confirmed using gray relational analysis (GRA). The top three parameters with the highest correlation with the carbonated concrete kAu were porosity (gray relational grade γi = 0.789), median pore diameter by volume (γi = 0.763), and proportion of transition pore volume (γi = 0.827). Furthermore, the regression analysis showed a good linear relation between kAu and the important pore structural parameters.

Effect of Uniaxial Compressive Stress on Phase Transformation Kinetics of Low-Carbon Steel.

To attain microstructure transformation and the kinetics of phase transformation under stress during the annealing process, dilatometric curves of phase transformation for Q235 steel were tested using a Gleeble-3500 thermal-mechanical simulator under different uniaxial compressive stresses. The Johnson-Mehl-Avrami (JMA) model considering impingement correction was applied to study the phase-transformation kinetics during annealing. The results showed that the grain size increased with increasing uniaxial compressive stresses because it provided additional energy for grain growth. Furthermore, the interfacial migration velocity decreased with increasing stress owing to grain coarsening and a decrease in the density of the α/γ boundary. Meanwhile, the stress reduces the sum of the misfit accommodation energy and interface energy caused by the transformation, and the driving force required for the transformation of austenite to ferrite decreases. Hence, it was concluded that uniaxial compressive stress plays a complex role in the phase transformation, which inhibits interfacial migration and the transformation rate while providing additional energy for the transformation.

In Situ Observation of Thermoelastic Martensitic Transformation of Cu-Al-Mn Cryogenic Shape Memory Alloy with Compressive Stress.

The thermoelastic martensitic transformation and its reverse transformation of the Cu-Al-Mn cryogenic shape memory alloy, both with and without compressive stress, has been dynamically in situ observed. During the process of thermoelastic martensitic transformation, martensite nucleates and gradually grow up as they cool, and shrink to disappearance as they heat. The order of martensite disappearance is just opposite to that of their formation. Observations of the self-accommodation of martensite variants, which were carried out by using a low temperature metallographic in situ observation apparatus, showed that the variants could interact with each other. The results of in situ synchrotron radiation X-ray and metallographic observation also suggested there were some residual austenites, even if the temperature was below Mf, which means the martensitic transformation could not be 100% accomplished. The external compressive stress would promote the preferential formation of martensite with some orientation, and also hinder the formation of martensite with other nonequivalent directions. The possible mechanism of the martensitic reverse transformation is discussed.

Unique Role of Vimentin Networks in Compression Stiffening of Cells and Protection of Nuclei from Compressive Stress.

In this work, we investigate whether stiffening in compression is a feature of single cells and whether the intracellular polymer networks that comprise the cytoskeleton (all of which stiffen with increasing shear strain) stiffen or soften when subjected to compressive strains. We find that individual cells, such as fibroblasts, stiffen at physiologically relevant compressive strains, but genetic ablation of vimentin diminishes this effect. Further, we show that unlike networks of purified F-actin or microtubules, which soften in compression, vimentin intermediate filament networks stiffen in both compression and extension, and we present a theoretical model to explain this response based on the flexibility of vimentin filaments and their surface charge, which resists volume changes of the network under compression. These results provide a new framework by which to understand the mechanical responses of cells and point to a central role of intermediate filaments in response to compression.

Ultrafast Time-Resolved Pump-Probe Investigation of Nanosecond Extreme Ultraviolet-Light-Induced Damage Dynamics on B4C/Ru Nano-Bilayer Film.

An ultrafast time-resolved pump-probe setup with both high temporal and spatial resolution is developed to investigate the transient interaction between a nanosecond extreme ultraviolet (EUV) pulse and matter. By using a delayed femtosecond probe pulse, the pattern evolution of surface modification induced by an EUV pump at a wavelength of 13.5 nm can be imaged at different delay times, which provides deep insight into the EUV-induced damage dynamics and damage mechanisms. As a demonstration, single-shot EUV damage on a B4C(6.0 nm)/Ru(30.4 nm)/D263 nano-bilayer optical film is studied using this pump-probe method. A recoverable phenomenon is found during the evolution process of the dome-shaped damage region. This is explained by the elastic and plastic deformations resulting from the huge compressive stress difference at the Ru-substrate interface with the help of simulations on the thermal effects and mechanical responses. This damage mechanism is further proven by the complementary experiments at a higher EUV fluence at 13.5 nm.