Exploring optimal folic acid supplementation level for lactating-pregnant rabbit does with different litter size.

The lactation-pregnancy overlap in the industrialized 49-day breeding model increases nutritional demands for lactating-pregnant rabbit does. This study examined the effects of folic acid (FA) on the production performance and intestinal microflora of does with different litter size (LS, or number of kits). A total of 144 third-parity Hyplus does, aged 11 months and weighing approximately 5.00 ± 0.07 kg, were divided four treatment groups: control group (basal diet) and FA groups (basal diet + 15, 30, and 45 mg/kg FA). Does with LS of 5, 7, and 9 were slected after weaning. Our findings revealed that 1) Increased FA supplementation initially increased and then decreased the pre-lactation body weight of 21-day-old kits and prolactin (PRL) level of does, while showing an inverse trend for estrogen (E) level of does. Increased LS significantly (P < 0.05) correlated with reduced milk yield of does. There was a significant (P < 0.05) interaction between FA and LS affecting PRL, E, growth hormone (GH) levels of does, and the pre-lactation body weight of 21-day-old kits. 2) Increased FA supplementation initially increased and then decreased the post-lactation body weight of 21-day-old and 35-day-old kits, elevated plasma folic acid (PFA) level of does, and significantly (P < 0.05) impacted the pregnancy rate of does. Increased LS was associated with reduced post-lactation body weight in 21-day-old and 35-day-old kits (P < 0.05). There was a significant (P < 0.05) interaction between FA and LS affecting plasma insulin-like growth factor-1 (IGF-1), homocysteine (HCY), PFA levels of does, post-lactation body weight of 21-day-old kits, and weaning body weight of 35-day-old kits. 3) FA supplementation promoted the growth of Ruminococcaceae and Rikenella_RC9_gut_group in feces of does, indicating enhanced anti-inflammatory capabilities and crude fiber (CF) breakdown. In summary, FA supplementation improved conception rates, regulated lactation-related hormone synthesis and secretion, increased body weight of 21-day-old kits, and enhanced intestinal anti-inflammatory functions.

Differences in the regulation of soil carbon pool quality and stability by leaf-litter and root-litter decomposition.

Litter plays a crucial role in soil ecosystems. However, the differences in decomposition between leaf-litter and root-litter and their relative contributions to soil carbon pools and stability are not yet clear. Therefore, we conducted a 450-day in situ decomposition experiment in a semi-arid grassland to investigate the effects of soil biophysical and chemical properties on litter decomposition and to elucidate the dynamics of soil carbon pools during the decomposition process. The results showed that the decomposition rate (K) of leaf-litter was significantly higher than that of root-litter, and litter quality was the most important factor affecting the K of leaf-litter (58%) and root-litter (63%). Leaf-litter decomposition was more effective in promoting the increase in soil leucine aminopeptidase and ß-1,4-glucosidase activities, as well as the accumulation of microbial biomass carbon (MBC), particulate organic carbon (POC), and dissolved organic carbon (DOC), compared to root-litter. However, the difference in the impact of leaf-litter and root-litter on soil organic carbon (SOC) was not significant. The decomposition of leaf-litter contributed more significantly to enhancing the soil carbon pool management index (CPMI) compared to root-litter, with increases of 39% and 25%, respectively. In contrast, leaf-litter decomposition significantly reduced the mineral-associated organic carbon (MAOC) and the MAOC/POC ratio, while root-litter decomposition significantly increased the MAOC and MAOC/POC. Random forest, partial correlation, and path analysis indicated that the effects of leaf-litter and root-litter decomposition on CPMI were mainly regulated by decomposition time and soil carbon components, while the effects on MAOC/POC were mainly controlled by litter quality. The results demonstrate that both leaf-litter and root-litter can enhance soil carbon storage and CPMI, but root-litter may be more beneficial for soil carbon pool stability. These results further contribute to the understanding of the continuous system of litter-soil carbon pools.

Beach litter in the European Arctic: Accumulation patterns, likely sources and pathways.

Patterns of beach litter accumulation in the European Arctic was investigated by sampling beaches on the Norwegian mainland in the southern Barents Sea, on northern Novaya Zemlya (Russia), and the southern shore of the Svalbard archipelago. The coast of Finnmark county on the Norwegian mainland was considerably more polluted than the other regions. More than half the surveyed beaches there were more polluted than the "very clean" designation from the Clean Coast Index, compared to <20 % in the other regions. The mean litter density was 8 items m-1, compared to 0.6-1.2 items m-1 in the other regions. Litter from maritime sources was common, and regional patterns in beach litter densities mirror regional patterns in nearshore (<50 km) fishing vessel activities as indicated by analyses of AIS tracks. A review of previously conducted beach litter Deep Dive analyses also indicate prevalent Norwegian and Russian packaging, further supporting a predominance of local litter sources.

Restoration of soil multifunctional indicators requires more than thirty years in degraded shrubland of a semi-arid mountainous ecosystem.

Vegetation degradation in natural environments leads to considerable fluctuations in soil function indicators, particularly in the sensitive and delicate habitats of semi-arid regions. In this study, the dynamic of both litter and soil properties was examined in northern Iran, in sites with Crataegus melanocarpa and Berberis integerrima dominance. The chosen sites have been degraded in 1993 due to exploitation for fuel production. Litter and soil (0-10, 10-20 and 20-30 cm depths) samples were collected in sites that have been degraded [i.e., 10-years post-degradation (2003), 20-years post-degradation (2013), and 30-years post-degradation (2023)]. A site not to exposed to degradation (year 2023) was also included as a control. In total, 48 litter samples (4 study sites × 12 samples) and 144 soil samples (4 study sites × 3 depths × 12 samples) were collected. Our findings showed a significant reduction of approximately 60-70% in the chemical characteristics of the litter, such as phosphorus (P) and magnesium (Mg) after 10-years post-degradation (2003) compared to the control site. Soil fertility indicators were also reduced 2-6 times as a result of habitat degradation. Aligned with this, the biota population in the 10-year post-degradation site showed a decrease of 70-90% with respect to the control. Moreover, the densities of fungi and bacteria decreased by approximately 30%-70% and 45-80% respectively compared to the 30-year post-degraded site. The peak activation of soil enzymes including urease, acid phosphatase, arylsulfatase, and invertase was found in the site that was not subjected to degradation, being 3.5, 5.8, 6.7 and 6.7- times higher in comparison to the 10-year post-degradation site. Overall, the results showed that in the non-degraded ecosystems, the input of plant-based organic matter was accompanied by an increase in soil fertility, organism population and soil function. This pinpoints the importance of protecting plant species in these areas, while in degraded areas planting native species adapted to the region's climate and fertilization could significantly contribute to restoring these areas.

Country-wide assessment of plastic removal rates on riverbanks and water surfaces.

Plastic pollution in river basins poses a significant environmental challenge, particularly in Japan, located in the northwestern North Pacific, often referred to as a hotspot for plastics. This study quantified the annual recovery of anthropogenic plastic litter from riverbanks and water surfaces, utilizing a nationwide cleanup dataset provided by the Japanese Ministry of Land, Infrastructure, Transport and Tourism. The dataset includes the amount of litter recovered through volunteer and administrative cleanup campaigns from 2016 to 2020 in 109 Japanese river basins. Total plastic recovery ranged from 763 to 1177 tons/year, averaging 938 tons/year. Basin-specific recovery was associated with extreme weather events, such as typhoon approaches and heavy rain, which caused significant flood damage in local regions. The estimated annual plastic recovery was an order of magnitude smaller than the previously estimated annual emission of land-based plastic.

Bacterial population-level trade-offs between drought tolerance and resource acquisition traits impact decomposition.

Microbes drive fundamental ecosystem processes such as decomposition. Environmental stressors are known to affect microbes, their fitness, and the ecosystem functions that they perform, yet understanding the causal mechanisms behind this influence has been difficult. We used leaf litter on soil surface as a model in situ system to assess changes in bacterial genomic traits and decomposition rates over 18 months with drought as a stressor. We hypothesized that genome-scale trade-offs due to investment in stress tolerance traits under drought reduce the capacity for bacterial populations to carry out decomposition, and that these population-level trade-offs scale up to impact emergent community traits thereby reducing decomposition rates. We observed drought tolerance mechanisms that were heightened in bacterial populations under drought, identified as higher gene copy numbers in metagenome-assembled genomes. A subset of populations under drought had reduced carbohydrate-active enzyme genes which suggested - as a trade-off - a decline in decomposition capabilities. These trade-offs were driven by community succession and taxonomic shifts as distinct patterns appeared in populations. We show that trait-tradeoffs in bacterial populations under drought could scale up to reduce overall decomposition capabilities and litter decay rates. Using a trait-based approach to assess the population ecology of soil bacteria, we demonstrate genome-level trade-offs in response to drought with consequences for decomposition rates.

Assessing the spread of sulfachloropyridazine in poultry environment and its impact on Escherichia coli resistance.

Sulfachloropyridazine (SCP) is an antimicrobial (AM) commonly used in the poultry industry. This drug is excreted as the original compound, which may accumulate in litter. This work was done to assess whether SCP residues from droppings of broiler chickens that were treated with therapeutic doses of this drug spread into the production environment and to determine if these events were associated with the selection of resistant bacteria. To this end, broiler chickens were raised under controlled conditions, and their droppings and litter were processed to detect and identify SCP residues using an HPLC-MS/MS technique. This study selected Escherichia coli as an indicator bacterium for AM resistance. Its phenotypic resistance was determined using the Kirby-Bauer disk diffusion method, and its genotypic resistance was determined by performing a conventional PCR test. Our results showed that SCP residues did spread from the treated group to untreated sentinel groups because SCP residues in the litter reached levels up to 43.05 µg·kg-1 in a group placed immediately adjoining to the treated group, while another group placed 30 cm away showed a concentration of 29.79 µg·kg-1. Meanwhile, only trace concentrations were detected in droppings collected from sentinel groups. Of 239 strains of E. coli isolated from droppings, 12.13 % were resistant to sulfonamides, whereas 23.91 % of 92 E. coli isolated from broiler litter were resistant. The most prevalent resistance gene was the sul2 gene, both in droppings and litter, followed by the sul1 gene. The SCP concentrations were associated with the probability of E. coli being resistant to sulfonamides (p-value = 0.01). A Multiple Correspondence Analysis (MCA) also showed that phenotypic and genotypic resistances were associated and that both genes sul1 and sul2 would determine phenotypic resistance to sulfonamides in E. coli. The results presented in this study show that inedible by-products of the poultry industry are potentially a source of drug resistance that can spread from the animal production line to the environment, so awareness of the correct use of antimicrobials is essential to combat antimicrobial resistance.

Dominant Tree Species and Litter Quality Govern Fungal Community Dynamics during Litter Decomposition.

Litter decomposition is a crucial biochemical process regulated by microbial activities in the forest ecosystem. However, the dynamic response of the fungal community during litter decomposition to vegetation changes is not well understood. Here, we investigated the litter decomposition rate, extracellular enzyme activities, fungal community, and nutrient cycling-related genes in leaf and twig litters over a three-year decomposition period in a pure Liquidamabar formosana forest and a mixed L. formosana/Pinus thunbergii forest. The result showed that during the three-year decomposition, twig litter in the mixed forest decomposed faster than that in the pure forest. In both leaf litter and twig litter, ß-cellobiosidase and N-acetyl-glucosamidase exhibited higher activities in the mixed forest, whereas phosphatase, ß-glucosidase, and ß-xylosidase were higher in the pure forest. The fungal α-diversity were higher in both litters in the pure forest compared to the mixed forest, with leaf litter showing higher α-diversity than twig litter. Fungal species richness and α-diversity within leaf litter increased as decomposition progressed. Within leaf litter, Basidiomycota dominated in the mixed forest, while Ascomycota dominated in the pure forest. Funguild analysis revealed that Symbiotroph and ectomycorrhizal fungi were more abundant in the mixed forest compared to the pure forest. In the third-year decomposition, genes related to phosphorus cycling were most abundant in both forests, with the pure forest having a higher abundance of cex and gcd genes. Fungal community structure, predicted functional structure, and gene composition differed between the two forest types and between the two litter types. Notably, the fungal functional community structure during the first-year decomposition was distinct from that in the subsequent two years. These findings suggest that dominant tree species, litter quality, and decomposition time all significantly influence litter decomposition by attracting different fungal communities, thereby affecting the entire decomposition process.

Exotic mangrove Laguncularia racemosa litter input accelerates nutrient cycling in mangrove ecosystems.

Exotic plant litter presents different chemical and physical properties relative to native plant litter and alters ecosystem processes and functions that may facilitate exotic plant dispersal. However, these effects are largely unknown, especially within wetland ecosystems. This study examines whether introducing litter from the exotic mangrove Laguncularia racemosa could result in (1) accelerated community litter decomposition rates and increased nutrient cycling rates and (2) microbial community structure changes in the invaded areas. A single decomposition experiment using litterbags was conducted to examine the short-term effects of L. racemosa litter in the native mangrove forest ecosystem. The soil nutrients and microbial communities of Rhizophora stylosa, L. racemosa, and mixed forests were also compared to explore the long-term cumulative effects of L. racemosa litter in native ecosystems. The results indicated that L. racemosa has lower-quality leaf litter than R. stylosa and a significantly faster decomposition rate. This may result from changes in the soil microbial community structure caused by L. racemosa leaf litter input, which favors the decomposition of its own litter. Both the short-term and cumulative effect experiments demonstrated that L. racemosa leaf litter significantly increased the relative abundance of microbes related to litter decomposition, such as Proteobacteria and Bdellovibrionota, and enhanced the alpha diversity of soil fungi, thus creating a microbial environment conducive to L. racemosa leaf litter decomposition. Moreover, the accumulation of soil nutrients was lower under L. racemosa than under R. stylosa over several years. This may be related to the more rapid growth of L. racemosa, which causes soil nutrient absorption and storage within the plant tissues, thereby reducing the soil nutrient content. Inputting exotic mangrove L. racemosa leaf litter reduced the soil blue carbon content, potentially adversely affecting global climate change. L. racemosa may employ a unique strategy to lower soil nutrient levels in native mangroves based on its low-quality leaf litter, thereby weakening the competitive ability of native plants that are intolerant to low-nutrient conditions and enhancing its own competitive advantage to further spread into these areas. In summary, the input of exotic L. racemosa leaf litter accelerates nutrient cycling in local mangroves.

Beached Plastic Debris Index; a modern index for detecting plastics on beaches.

Plastic pollution on shorelines poses a significant threat to coastal ecosystems, underscoring the urgent need for scalable detection methods to facilitate debris removal. In this study, the Beached Plastic Debris Index (BPDI) was developed to detect plastic accumulation on beaches using shortwave infrared spectral features. To validate the BPDI, plastic targets with varying sub-pixel covers were placed on a sand spit and captured using WorldView-3 satellite imagery. The performance of the BPDI was analysed in comparison with the Normalized Difference Plastic Index (NDPI), the Plastic Index (PI), and two hydrocarbon indices (HI, HC). The BPDI successfully detected the plastic targets from sand, water, and vegetation, outperforming the other indices and identifying pixels with <30 % plastic cover. The robustness of the BPDI suggests its potential as an effective tool for mapping plastic debris accumulations along coastlines.

Biodegradation of untreated plasticizers-free linear low-density polyethylene films by marine bacteria.

Polyethylene significantly contributes to marine plastic pollution. This study focuses on isolating bacteria from sea water and microplastic samples collected from the Tyrrhenian Sea and evaluating their ability to degrade virgin plasticizers-free linear low-density polyethylene (LLDPE) films. The isolates grew on the plastic film under aerobic conditions in shaken flasks leading to LLDPE mass losses of up to 2.597 ± 0.971 % after 60 days incubation. Biofilm formation on the film surface was confirmed by adhered protein quantification while film surface erosion and appearance of functional groups were revealed using SEM and FTIR analyses confirming biodegradation capabilities especially for isolates Bacillus velezensis MT9, Vreelandella venusta MT1 and Vreelandellatitanicae MT11. This is the first report on the biodegradation of plasticizers-free non pretreated LLDPE films by marine Bacillus sp. and Vreelandella sp.; most of the LLDPE biodegradation studies have been so far performed on plasticizer containing, pre-treated, or naturally weathered films.

Genome-Wide Association Study of Reproductive Traits in Large White Pigs.

(1) Background: Reproductive performance is crucial for the pork industry's success. The Large White pig is central to this, yet the genetic factors influencing its reproductive traits are not well understood, highlighting the need for further research. (2) Methods: This study utilized Genome-Wide Association Studies to explore the genetic basis of reproductive traits in the Large White pig. We collected data from 2237 Large White sows across four breeding herds in southern China, focusing on eight reproductive traits. Statistical analyses included principal component analysis, linkage disequilibrium analysis, and univariate linear mixed models to identify significant single-nucleotide polymorphisms and candidate genes. (3) Results: Forty-five significantly related SNPs and 17 potential candidate genes associated with litter traits were identified. Individuals with the TT genotype at SNP rs341909772 showed an increase of 1.24 in the number of piglets born alive (NBA) and 1.25 in the number of healthy births (NHBs) compared with those with the CC genotype. (4) Conclusions: The SNPs and genes identified in this study offer insights into the genetics of reproductive traits in the Large White pig, potentially guiding the development of breeding strategies to improve litter size.

High microplastic pollution in marine sediments associated with urbanised areas along the SW Bulgarian Black Sea coast.

This study aims to provide baseline data on microplastic (MP) concentrations in beach and seabed sediments of the SW Black Sea coast, and to identify possible sources of MP pollution in the area. MP concentrations in beach sediments in urbanised coastal areas, as well as in all seabed sediments, were on average higher than in other parts of the Black Sea and most other European seas. Urban coastal areas showed the highest average MP concentrations (mean ± SD): 464.44 ± 272.50 MP·kg-1 dry sediment in beach samples, 825.93 ± 396.30 MP·kg-1 dry sediment in seabed samples, while coastal sediments in an area with no local anthropogenic activities had significantly lower average MP concentrations: 84.44 ± 147.57 MP·kg-1 in beach samples, and 550.62 ± 245.71 MP·kg-1 in seabed samples. Possible identified sources of MPs include sewage water discharges, urban waste, coastal fisheries and aquaculture, as well as accumulation of marine litter on beaches and in adjacent offshore areas, serving as sources of secondary MP pollution.

Incorporation of plastic debris into the nests of great cormorants (Phalacrocorax carbo sinensis) in the Gulf of Finland.

Several bird species are known to utilise marine debris among their nest materials in different parts of the world. This study examines the prevalence of debris in cormorant nests in the Gulf of Finland, focusing mainly on plastic debris. 200 nests were sampled on four different nesting islets, and plastic debris in the nest was counted and classified according to their type, colour, origin and polymer type. In total, 58 % of the nests contained debris (95 % plastics), but the prevalence of debris in the nests varied between the colonies. Core nests contained more debris than periphery nests. The relative number of threadlike plastics in the nests was higher than that in the surrounding environment, indicating active selection by cormorants. Based on the results, nest surveys could be a useful tool in evaluating the effectiveness of certain reduction measures aiming to tackle marine plastic pollution in the Baltic Sea.

Benthic litter in fishing grounds of the Northern Adriatic: Role of the trawling fleet as cleaners of the seafloor.

This study represents the baseline of estimation of the potential service provided by fishermen as "cleaners of the sea". The amount, composition and depth distribution of marine litter in fishing grounds of the Northern Adriatic seafloor has been investigated through the fishing for litter (FFL) scheme. Passive FFL campaigns were carried out by trawlers from two of the most important fishing ports in the northern Adriatic, Chioggia and Goro, from May 2020 to May 2021. Over the course of 256 days of fishing, over 6 tons of litter were removed from 265 km2 of seafloor. Abandoned, lost and derelict fishing gears (ALDFG) were the most represented litter category (48 % of the total litter), and of these 67 % were plastic ALDFG (mostly mussel socks and fishing nets). Fouling on plastic waste was analyzed to determine the fraction of collected litter items that could be destinated to recycling. Only a small percentage of the plastic litter analyzed was "clean" from adherent and/or encrusting organisms. Approximately 2.4 tons of plastic were recovered, but, due to the biological colonization of surfaces, they cannot be recycled by using the technologies present in the area.

The use of partially slatted floor designs as an alternative to littered systems in broiler chickens. I. The effects on the performance, slaughter and carcass traits.

In this study, the effects of fully littered, slatted and their varying combinations on growth, performance, carcass and meat quality characteristics in broiler chickens were investigated. In the experiment, five treatment groups were formed as fully littered (FL), fully slatted (FS), 1/3 littered + 2/3 slatted (2/3 SF), 1/2 littered + 1/2 slatted (1/2SF), 2/3 littered + 1/3 slatted (1/3 SF). Each treatment group included 5 replicates (24 chicks in each) and a total of 600 male-female mixed fast growing broiler chicks (Ross-308) were used during 6 weeks. Weekly body weight (BW, g), feed intake (FI, g), feed conversion ratio (FCR, g.feed intake/g.weight gain), livability (%) and EPEF (European Production Efficiency Factor) were determined as performance characteristics. Carcass yield (CY, %), and the percentages of abdominal fat (AFP, %), edible internal organs (EIOP, %), gizzard (GP, %), breast (BRP, %) and thigh (TP, %) meat were the carcass characteristics. Meat quality measured by pH and color (L*: lightness, a*: redness, b*: yellowness) of breast and thigh meat, and occurrence of white striping and hemorrhaging in breast meat. The weekly BW differences among the floor treatments were significant (P < 0.01), and 1/3SF broilers were the heaviest at slaughter age (3311.6 g). The 6-week FCR in FS broilers was significantly worse than the others (1.471), but FI, livability and EPEF were similar among treatments. AFP tended to be higher in FL birds (P = 0.066), while CY, EIOP, GP, BRP and TP did not differ between groups. Breast pH was similar between treatments, with 1/2SF showing the highest (6.34) and FL the lowest (6.24) breast pH (P < 0.01). The partially slatted systems caused lighter, redder and yellower color values in breast meat (P < 0.01) than the fully littered or slatted systems, but did not change the color of the thigh meat. White striping and hemorrhaging in breast meat were not affected by the floor treatments. This study suggests that the use of partially slatted systems in broiler chickens improves meat color without compromising performance traits (body weight, feed efficiency, EPEF), litter moisture and slaughter traits and these systems could be an alternative to fully littered systems.

Study of pollution status in urban environment caused by attitude and waste littering behavior of citizens.

Littering behavior is one of the main challenges in waste management, especially in big cities. In this study, density and composition of litter in Tehran was investigated and the urban pollution status was calculated by using the clean environment index. The results showed that the average density of litter in the studied locations was 0.182 number/m2. Spatial variation in the litter density showed that the average density in commercial and residential land-uses was 0.22 and 0.14 number/m2, respectively. Cigarette butts (29%), bottle caps (18%), and paper receipts (10%) consisted the largest ratio of the litter composition in the studied locations. Clean environment index for the studied locations was calculated in the range of 2.34 to 10.42. Based on this, 30% of the studied locations were in a clean status and 70% were in a polluted and worse status. The litter density in the city, interviews with citizens, and the results of previous studies showed insufficient awareness of citizens about the consequences of littering in the urban environment, which led to the prevalence of littering behavior. So, the littering behavior by citizens has caused the urban environment pollution and increased health risk. Educating citizens and modifying their behavior to avoid littering is necessary to improve the observed status.

Assessment of transboundary macro-litter on the remote island of Andaman and Nicobar: Unveiling the governing factors and risk assessment.

The increasing occurrence of mismanaged plastic litter along India's coastline and the ominous challenges it poses to biodiversity and ecosystem health is a growing environmental concern. To address this issue, we comprehensively investigated the abundance, composition, and probable sources of marine litter on North Cinque Island, a remote uninhabited island in the Andaman and Nicobar archipelago, Bay of Bengal. This island is a designated wildlife sanctuary and serves as an important nesting site for Green, Hawksbill and Leatherback turtles. A total of 6227 litter items were enumerated, with an average concentration of 0.12 items/m2, representing 20 diverse litter types, with plastic dominating the litter composition (86 %). The cleanliness and environmental hazards of the coast due to the litter were assessed using different indices such as the Clean Coast Index (CCI), Plastic Accumulation Index (PAI), Hazardous Item Index (HII), and Clean Environment Index (CEI). CCI indicates the moderately clean-to-clean status of the surveyed sites. PAI points to low to moderate accumulation of plastic litter. HII of all five coasts fell in category II, suggesting a moderate abundance of hazardous items that can inflict injuries to the foraging turtle and their hatchlings. The CEI articulates the moderately clean to very clean status of the sites. Litter brand audit suggests a considerable amount of stranded litter on the coasts was transboundary and originated from six Indian Ocean Rim Countries (IORC), namely Thailand, Myanmar, Malaysia, Indonesia, Sri Lanka, and UAE. Joint solid waste management by the IORC is the need of the hour to avert litter accumulation on the pristine, remote islands.

Occurrence, Multidrug Resistance, and Multilocus Sequence Typing of Extended-Spectrum Beta-Lactamase/AmpC-Producing Escherichia coli from Farmed Eggs.

The present study evaluated the occurrence, antibiogram profile, and sequence types (STs) of multidrug resistant (MDR) Escherichia coli from freshly laid eggs (n = 480), feed (n = 24), water (n = 24), poultry droppings (n = 24), and hand swab samples (n = 10) collected from 24 deep litter (DL) and caged poultry layer farms (12 per category) across Punjab, India. The overall E. coli contamination rate in DL and cage farms was 32% (95% confidence intervals [CI], 26.6-37.8%) and 16.7% (95% CI, 12.6-21.6%), respectively. The logistic regression analysis revealed that the DL system had higher odds of occurrence (odds ratio [OR]) of extended-spectrum beta-lactamase (ESBL) (2.195, 95% CI, 1.065, 4.522) and ESBL/AmpC coproducers (2.69, 95% CI, 1.122, 6.45) compared to the cage system. Additionally, isolates from the DL were 4.065 (95% CI, 1.477, 11.188) times more tetracycline resistant compared to the latter; however, resistance to amoxyclavulanate (OR, 0.437; 95% CI, 0.209, 0.912), and ampicillin (OR, 0.343; 95% CI, 0.163, 0.720) was lesser in DL system. Notably, around 97.7% and 87.2% of the isolates from the DL and cage system were MDR, with the DL system having 6.439 (95% CI, 1.246, 33.283) times more chances of harboring MDR E. coli. Additionally, among the resistance genes, the DL system demonstrated significantly high presence of blaAmpC (56%), qnrA/B/S (42.3%), and tetA/B (30.6%). Furthermore, multilocus sequence typing of 11 MDR isolates (n = 5, DL, and 6, cage) revealed the presence of 10 STs, of which ST10, ST155, and ST156 were found to be of public health importance. Therefore, the present study highlights the burden of MDR, ESBL, and AmpC-producing E. coli on poultry eggs and farm environment, which could be carried over to human handlers and consumers upon direct contact during handling and processing.

Fate of fluoroquinolones associated with antimicrobial resistance in circular periurban agriculture.

Animal antibiotic use contributes to antimicrobial resistance (AMR) in humans. While animal manure benefits soil fertility, it also acts as hotspot for antibiotic residues, antibiotic-resistant bacteria, and their genes. Amending soils with poultry litter is recognized as "magic" among horticulture farmers and it remains a common practice globally. However, this poses a risk especially in countries where prophylactic use of antibiotics is allowed. In Argentina, fluoroquinolones are used in this way besides being listed as essential medicines and classified as "watch" by the World Health Organization. Antibiotic selective pressure can favour AMR in the environment but the fate of antibiotic residues and AMR dissemination from these practices remains poorly understood. Our research addresses this gap with a biological model tracing fluoroquinolones from poultry to soil to lettuce and tracking anthropogenic AMR with the proposed biomarker genes sul1 and intI1. Fresh poultry litter was stored for six months before application in a horticulture field experiment. The experiment included control and manured plots where lettuce was cultivated till harvest. Enrofloxacin concentration was 7.3 µg/kg in fresh poultry litter, while its metabolite ciprofloxacin was 39.22 µg/kg after storage. Although no fluoroquinolones were detected in soils, lettuce from manured plots contained enrofloxacin and ciprofloxacin at 14.97 and 9.77 µg/kg, respectively, providing evidence of fluoroquinolone bioaccumulation in plants. Abundance of sul1 and intI1 in poultry litter was not affected by storage. Manured soils showed better soil quality than controls, but sul1 gene abundance was 1.6 times higher, reaching 7.61 Log sul1/g soil. A less sensitive, but significant effect was registered for intI1. These findings show that static storage is insufficient to stop the transmission of antibiotics and AMR biomarkers from poultry to horticulture. Amending soil with industrial poultry litter contributes to pollution with these emergent contaminants and risks human antibiotic exposure through fresh vegetables.