RESUMO
A Gram-stain-positive, rod-shaped, aerobic, motile bacterium, J379T, was isolated from radioactive water spring C1, located in a former silver-uranium mine in the Czech Republic. This slow-growing strain exhibited optimal growth at 24-28â°C on solid media with <1â% salt concentration and alkaline pH 8-10. The only respiratory quinone found in strain J379T was MK-7(H4). C18â:â1 ω9c (60.9â%), C18â:â0 (9.4â%), C16â:â0 and alcohol-C18â:â0 (both 6.2â%) were found to be the major fatty acids. The peptidoglycan contained directly cross-linked meso-diaminopimelic acid. Phylogenetic reconstruction based on the 16S rRNA gene sequences and the core-genome analysis revealed that strain J379T forms a separate phylogenetic lineage within the recently amended order Solirubrobacterales. A comparison of the 16S rRNA gene sequences between strain J379T and other members of the order Solirubrobacterales showed <96â% similarity. This analysis revealed that the closest type strains were Parviterribacter kavangonensis D16/0 /H6T (95.2â%), Capillimicrobium parvum 0166_1T (94.9â%) and Conexibacter arvalis KV-962T (94.5â%). Whole-genome analysis showed that the closest type strain was Baekduia soli BR7-21T with an average nucleotide identity of 78â%, average amino acid identity of 63.2â% and percentage of conserved proteins of 48.2â%. The G+C content of the J379T genomic DNA was 71.7âmol%. Based on the phylogenetic and phylogenomic data, as well as its physiological characteristics, strain J379T is proposed to represent a type strain (DSM 113746T=CCM 9300T) of Svornostia abyssi gen. nov. sp. nov. within the family Baekduiaceae.