Cysteinyl leukotriene 1 receptor expression in nasal polyps.

Cysteinyl leukotrienes (CysLTs) have been implicated in the pathogenesis of chronic rhinosinusitis (CRS). This study was undertaken to better understand the role of CysLTs in the development of CRS through 1). assessment of the pattern of expression of CysLT1 receptor in nasal polyps from patients with CRS and 2). correlation of expression of CysLT1 receptor with clinical features. Expression of CysLT1 receptor was evaluated immunohistochemically in nasal polyps from 20 patients with CRS and nasal mucosa from 10 control subject undergoing plastic surgery of the nose. Patients with CRS showed significantly higher expression of CysLT1 receptor as compared with the control group (p<0.05). The expression of CysLT1 receptor in sub-epithelial inflammatory infiltrates tended to be higher in patients with CRS and allergy as compared with patients with CRS but without allergy (p=0.07). In particular, the expression of CysLT1 receptor in sub-epithelial inflammatory infiltrates was significantly greater in 3 patients with CRS and drug allergy as compared with patients with CRS but without drug allergy (p=0.03). Increased expression of CysLT1 receptor in inflamed mucosa of nasal polyps in patients with allergy might suggest particular role of CysLTs in the pathogenesis of nasal polyps in this group of patients.

[The usefulness of evaluation of: ferritin, ultrasensitive CRP and tissue specific polypeptide 18th (TPS) in assessment of therapy efficacy in patients with nasal polyps]. / Wartosc oznaczen wybranych parametrów diagnostycznych: ferrytyny, ultraczulego CRP i rozpuszczalnych fragmentów cytokeratyny 18. (TPS) w ocenie skutecznosci terapii chorych z polipami nosa.

BACKGROUND: Chronic rhinosinusitis with nasal polyps is social, clinical and cost-effective problem, by reason of bothersome symptoms, chronic nature of the disease, tendency to recur and lack of satisfying treatment. AIM: The aim of this study is assessment of suitability of hsCRP, ferritin and blood levels in nasal polyps patients in evaluation of treatment efficacy. METHODS: The study enrolled 38 patients between 20 and 68 years of age. Patients were divided into 2 groups. Levels of ultrasensitive CRP ferritin and TPS have been measured in all patients. The ultrasensitive CRP levels have been measured by chemiluminescence method. Ferritin levels have been measured by MEIA method. The TPS levels have been measured by chemiluminescence method. RESULTS: Comparison of mean ferritin levels in both study groups in each stage of observation shows the significant difference of mean values in only 6 weeks after surgery. Mean ferritin level is significantly lower in group I than in group II (p<0.05). Mean hsCRP levels vary from one corresponding to ferritin levels. Statistically significant difference between study groups in 2nd and 6th week after surgery has been ascertained (p<0.05). Similarly, like in ferritin levels, the TPS levels are significantly different in 6th week after surgery. CONCLUSIONS: Analysis of ferritin, hsCRP and TPS serum levels indicates that these may be useful in assessment of treatment efficacy in patients with nasal polyps. Rise of the chosen inflammatory state parameter level in the postoperative monitoring and anti-inflammatory treatment introduction in nasal polyps patients may inhibit the recurrence of the disease.

[Influence of complex deacon-tetra (N1,3-triazole,кN2) copper (II) on the barrier and antioxidant pro people with nasal polyps]. / Wplyw kompleksu diaka-tetra (N1,3-triazol,кN2) miedz (II) na bariere pro- i antyoksydacyjna osób z polipami nosa.

INTRODUCTION: The aim of this study was synthesized properties 1,2,4-triazole complex ion Cu (II) to determine their potential antioxidant properties, further indication of activity of antioxidant enzymes: catalase (CAT), glutathione peroxidase (GPX) and superoxide dismutase (Cu-Zn SOD) in patients with nasal polyps and the control group. MATERIAL AND METHODS: The study was conducted in a group of 30 patients with nasal polyps aged 48±13.50 (test 1 - group with a compound, test 2 - no relation), and 30 in the control group aged 48±17.90 (control 3 - group with a compound, control 4 - no relation). Blood samples were collected from the antecubital vein into tubes with anticoagulant (heparin sodium). The activity of catalase, superoxide dismutase and glutathione peroxidase were determined in erythrocytes of patients with nasal polyps and the control group (those without inflammatory conditions. RESULTS: We found that patients with nasal polyps show a decrease in the activity of antioxidant enzymes compared to control: CAT (control 3 - 13.78±6.71 BU/gHb; test 1 - 8.52±7.96 BU/gHb), SOD (control 3 - 1377,614.93±93.44 U/gHb/100ml; test 1 - 867,270.59±49 U/gHb/100ml) and GPX (control 3 - 101.62±8.23 U/gHb; test 1 - 72.97±14.34 U/gHb). Simultaneously, we observed that increasing the activity of antioxidant enzymes compared in the groups control 4 to test 2 (in groups which do not given coordination compound Cu (II): CAT (control 4 - 13.38±7.60 BU/gHb; test 2 - 10.12±3.62 BU/gHb) SOD (control 4 - 1138.88±490.10 U/gHb/100ml; test 2 - 1283.85±439.87 U/gHb/100ml) and GPX (control 4 - 65.18±20.94 U/gHb; test 2 - 90.27±19.42 U/gHb). CONCLUSIONS: The study shows that the complex deacon-tetra (N1,3-triazole,кN2) copper (II) affects the activity of antioxidant enzymes CAT, SOD and GPX.