Evaluation of the Main Macro-, Micro- and Trace Elements Found in Fallopia japonica Plants and Their Traceability in Its Honey: A Case Study from the Northwestern and Western Part of Romania.

Fallopia japonica (Japanese knotweed, Reynoutria japonica or Polygonum cuspidatum) is considered an extremely invasive plant worldwide and a bioindicator of heavy metals. Yet, its potential as a crop for honeybees is still underevaluated. This study employs atomic absorption spectrometry to quantitatively analyze the concentration of macro-elements, namely, calcium (Ca), potassium (K) and magnesium (Mg); micro-elements, such as copper (Cu), iron (Fe), manganese (Mn) and selenium (Se); and trace elements, i.e., cadmium (Cd), chromium (Cr), nickel (Ni) and lead (Pb) in different anatomic parts of Fallopia japonica (FJ) plants (roots, rhizomes, stems, leaves) and their traceability into honey. This research encompasses a thorough examination of samples collected from the northwestern and western part of Romania, providing insights into their elemental composition. The results showed that the level of trace elements decreases in terms of traceability in honey samples (Pb was not detected in any of the analyzed samples, while Cd had a minimum content 0.001 mg/kg), ensuring its quality and health safety for consumption. Moreover, the data generated can function as a valuable resource to explore the plant's positive eco-friendly impacts, particularly in relation to its honey.

Is residential proximity to polluted sites during pregnancy associated with preterm birth or low birth weight? Results from an integrated exposure database in North Carolina (2003-2015).

BACKGROUND: Preterm birth (PTB) and term low birth weight (LBW) have been associated with pollution and other environmental exposures, but the relationship between these adverse outcomes and specific characteristics of polluted sites is not well studied. OBJECTIVES: We conducted a retrospective cohort study to examine relationships between residential proximity to polluted sites in North Carolina (NC) and PTB and LBW. We further stratified exposure to polluted sites by route of contaminant emissions and specific contaminants released at each site. METHODS: We created an integrated exposure geodatabase of polluted sites in NC from 2002 to 2015 including all landfills, Superfund sites, and industrial sites. Using birth certificates, we assembled a cohort of 1,494,651 singleton births in NC from 2003 to 2015. We geocoded the gestational parent residential address on the birth certificate, and defined exposure to polluted sites as residence within one mile of a site. We used log-binomial regression models to estimate adjusted risk ratios (aRR) and 95% confidence intervals (CI). Binomial models were used to estimate adjusted risk differences (aRD) per 10,000 births and 95% CIs for associations between exposure to polluted sites and PTB or LBW. RESULTS: We observed weak associations between residential proximity to polluted sites and PTB [aRR(95% CI): 1.07(1.06,1.09); aRD(95% CI): 61(48,74)] and LBW [aRR(95% CI): 1.09(1.06,1.12); aRD(95% CI): 24(17,31)]. Secondary analyses showed increased risk of both PTB and LBW among births exposed to sites characterized by water emissions, air emissions, and land impoundment. In analyses of specific contaminants, increased risk of PTB was associated with proximity to sites containing arsenic, benzene, cadmium, lead, mercury, and polycyclic aromatic hydrocarbons. LBW was associated with exposure to arsenic, benzene, cadmium, lead, and mercury. SIGNIFICANCE: This study provides evidence for potential reproductive health effects of polluted sites, and underscores the importance of accounting for heterogeneity between polluted sites when considering these exposures. IMPACT STATEMENT: We documented an overall increased risk of both PTB and LBW in births with gestational exposure to polluted sites using a harmonized geodatabase of three site types, and further examined exposures stratified by site characteristics (route of emission, specific contaminants present). We observed increased risk of both PTB and LBW among births exposed to sites with water emissions or air emissions, across site types. Increased risk of PTB was associated with gestational proximity to sites containing arsenic, benzene, cadmium, lead, mercury, and polycyclic aromatic hydrocarbons; increased risk of LBW was associated with exposure to arsenic, benzene, cadmium, lead, and mercury.

Knufia obscura sp. nov. and Knufia victoriae sp. nov., two new species from extreme environments.

Six strains of black meristematic fungi were isolated from Antarctic soils, gasoline car tanks and from the marine alga Flabellia petiolata. These fungi were characterized by morphological, physiological and phylogenetic analyses. According to the maximum-likelihood analysis reconstructed with ITS and LSU sequences, these strains belonged to the genus Knufia. Knufia obscura sp. nov. (holotype CBS 148926) and Knufia victoriae sp. nov. (holotype CBS 149015) are proposed as two novel species and descriptions of their morphological, physiological and phylogenetic features are presented. Based on the maximum-likelihood analyses, K. obscura was closely related to Knufia hypolithi (99 % bootstrap support), while K. victoriae clustered in the clade of Knufia cryptophialidica and Knufia perfecta (93 % bootstrap support). Knufia victoriae, recorded in Antarctic soil samples, had a psychrophilic behaviour, with optimal growth between 10 and 15 °C and no growth recorded at 20 °C. Knufia obscura, from a gasoline car tank and algae, displayed optimal growth between 20 and 25 °C and was more tolerant to salinity than K. victoriae.

Pollution pressure and soil depth drive prokaryotic microbial assemblage and co-occurrence patterns in an organic polluted site.

Organic polluted sites have become a global concern of soil contamination, yet little is known about microbial vertical distribution and community assembly in organic polluted sites. Here, high-throughput sequencing technology was employed to investigate prokaryotic microbial diversity and community assembly along soil profile in an abandoned chemical organic contaminated site. Results showed that there was no significant difference (P > 0.05) observed in microbial alpha diversity among different soil layers, whereas the structure of microbial communities presented significantly different (P < 0.05) in the superficial layer (0-0.5 m) compared with intermediate (1-1.5 m) and bottom (2.5-3 m) layers. Soil prokaryotic microbial community evolved to possess the potential of degrading organic pollutants under long-term organic pollution stress. A relatively homogeneous environment created by the organic polluted site mainly induced the ecological process of homogeneous selection driving community assembly, while dispersal limitation gained importance with the increase of soil depth. Organic contaminants were identified as the key driver of destabilizing co-occurrence networks, while the frequent cooperative behaviors among species could combat organic pollution stress and sustain prokaryotic community stability. Collectively, pollution pressure and soil depth jointly affected prokaryotic microbial assemblage and co-occurrence that underpinned the spatial scaling patterns of organic contaminated sites microbiota.

Bioaccessibility of Metals in Soils at Typical Legacy Industrial Sites: In Vitro Evaluation Using Physiologically-Based Extraction.

Risk assessment of soil metal pollution based on total metal contents might give overestimates by neglecting the bioaccessibility of the pollutants to soil biota. Physiologically-based extraction tests (PBET) are in vitro methods for evaluation of bioaccessibility of soil pollutants. A total of 27 soil samples collected from four types of legacy industrial site representing metal smelting, lead-acid battery factories, chemical plants and steel plants were analyzed for the bioaccessibility of six potentially toxic metals using a PBET method. The metal pollutants at the industrial sites depended on the former industrial processes and emissions. The highest proportions of gastric phase and intestinal phase in these soil samples were 43.9% for Cd and 27% for Cu, respectively. Factors affecting metal bioaccessibility included type of industry and soil properties. The soils at a lead-acid battery factory showed relatively high bioaccessibility of Pb, Zn and Cd and those at the steel plant showed relatively low metal bioaccessibility. Soil organic matter and clay contents were positively related to metal bioaccessibility but soil pH and CEC showed negative relationships. Further studies are recommended to determine the speciation of the bioaccessible metals in these soils.

Improving the assessment of polluted sites using an integrated bio-physico-chemical monitoring framework.

Soil - water pollution resulting from anthropogenic activities is a growing concern internationally. Effective monitoring techniques play a crucial role in the detection, prevention, and remediation of polluted sites. Current pollution monitoring practices in many geographical locations are primarily based on physico-chemical assessments which do not always reflect the potential toxicity of contaminant 'cocktails' and harmful chemicals not screened for routinely. Biomonitoring provides a range of sensitive techniques to characterise the eco-toxicological effects of chemical contamination. The bioavailability of contaminants, in addition to their effects on organisms at the molecular, cellular, individual, and community level allows the characterisation of the overall health status of polluted sites and ecosystems. Quantifying bioaccumulation, changes to community structure, faunal morphology, behavioural, and biochemical responses are standard procedures employed in biomonitoring studies in many High-Income Countries (HICs). This review highlights the need to integrate biomonitoring tools alongside physico-chemical monitoring techniques by using 'effect-based' tools to provide more holistic information on the ecological impairment of soil-water systems. This paper considers the wider implementation of biomonitoring methods in Low to Middle Income Countries (LMICs) and their significance in pollution investigations and proposes an integrated monitoring framework that can identify toxicity drivers by utilising 'effect-based' and 'risk-based' monitoring approaches.

Evaluating the status of phytochemicals within Catharanthus roseus due to higher metal stress.

Catharanthus roseus (Madagascar periwinkle) is the source of terpenoid indole alkaloids which are minor products in the plants, making their isolation elaborate and costly. The low yield of the alkaloids in the plant combined with their high market price made Catharanthus roseus an important model system for plant biotechnology and secondary metabolism studies. This study highlights the responses of Catharanthus roseus due to metal stress under pot culture conditions. It was observed that metals like zinc and copper showed a positive effect on accumulation of few secondary metabolites in Catharanthus roseus. Accumulation of total alkaloids, phenolics and flavonoids in the plants were observed to increase with increasing concentrations of these metal treatments given to growing plants. Similarly, plants grown in industrially contaminated soils also showed varying alkaloid, phenolics and flavonoid accumulation in comparison to those grown in garden soil. Higher metal contaminations in the soil lead to higher production of certain phytochemicals in different plant parts.

Risk valuation of ecological resources at contaminated deactivation and decommissioning facilities: methodology and a case study at the Department of Energy's Hanford site.

Many countries are faced with monumental cleanup tasks remaining from World War II and the Cold War and consistent methodologies are essential to assess the risk from pollutants and the risk from cleanup. In the USA, the Department of Energy (DOE), and other federal and state agencies need to be able to rapidly evaluate the risk to ecological resources for remediation projects. While ecological risk assessments for radionuclides and other contaminants can be performed for different species, evaluations of species assemblages, communities, and ecosystems is more difficult. We summarize an evaluation method for ecological resources on individual remediation units that will allow comparison among a large number of units and that can be modified and applied to the DOE complex-wide. We evaluated the deactivation and decommissioning (D & D) facilities at the Hanford site as case studies. Remediation of these sites has the potential to provide harm to, or increase the value of, ecological resources during and after the process. The evaluation method includes three categories: (1) general steps, (2) ecological descriptions, and (3) ecological ratings. The general steps include identifying the categories of resources (level of resource value), identifying the units to be evaluated (e.g., remediation units), identifying a reasonable ecological buffer around the evaluation units, identifying the remediation options (from milestones or other agreements), and developing a rating scale. Ecological descriptions include identifying previous ecological values of specific areas on the evaluation unit, conducting field studies to assess the current conditions, and summarizing the percent of each resource value on the evaluation unit and buffer area. The ecological risk of harm is determined by using the rating scale to evaluate the potential harm to the ecological (and eco-cultural) resources on each evaluation unit currently, during remediation, and in the post-remediation phase. Currently, the risks (potential harm) to ecological resources on the D & D facilities at the Hanford site are non-discernible, but they increase to very high (for reactors) during remediation when there is physical disruption, increased traffic and personnel, and possible increased contamination. Following remediation, the potential harm to ecological resources is low, and the value may be increased due to restoration of native vegetation on sites that were largely industrial prior to remediation. These methods provide managers, regulators, tribes, and the general public with assurance that ecological and eco-cultural resources and the environment are being protected during and following remediation.

Deposition of absolute and relative airborne metals on eggshells: a field study.

A biomonitoring field study was conducted to test the deposition of airborne metals on chicken eggshell. The goal was to correlate PM2.5 particle concentration in air, absolute (metals in air) and relative (metals in PM2.5 particles) metals, and metal accumulation on eggshell. The PM2.5 sample was collected for 8 h at different air pollution sites as well as an unpolluted site with glass fiber filters via a fine particulate sampler (with and without addition of eggshell). The PM2.5 particle concentration was high at a cement factory site and low at a sugar factory site. The highest absolute total mass of metal (total mass of all metals in air) was found in the traffic site, and the highest relative total mass of metal (total mass of all metals in PM2.5 particles) was found at the tannery factory. The accumulation of Zn and Pb in eggshell was high at the tannery and sugar factory sites, and Fe was high in the other areas. Three common metals including Cd, As, and Pb were found with significant positive correlation between absolute and relative metals with the metals accumulated in eggshell. The results concluded that the eggshells might be useful tools for monitoring the airborne metals.

Systematic literature review of reproductive outcome associated with residential proximity to polluted sites.

This study aims to assess the evidence on adverse pregnancy outcome associated with living close to polluted industrial sites, and identify the strengths and weaknesses of published epidemiological studies. A systematic literature search has been performed on all epidemiological studies published in developed countries since 1990, on the association between residential proximity to industrial sites (hazardous waste sites, industrial facilities and landfill sites) and adverse pregnancy outcome (low birth weight, preterm birth, small for gestational age, intrauterine growth retardation, infant mortality, congenital malformation). Based on 41 papers, our review reveals an excess risk of reproductive morbidity. However, no studies show significant excess risk of mortality including fetal death, neonatal or infant mortality and stillbirth. All published studies tend to show an increased risk of congenital abnormalities, yet not all are statistically significant. All but two of these studies revealed an excess risk of low birth weight. Results for preterm birth, small for gestational age and intrauterine growth retardation show the same pattern. There is suggestive evidence from the post-1990 literature that residential proximity to polluted sites (including landfills, hazardous waste sites and industrial facilities) might contribute to adverse reproductive outcomes, especially congenital malformations and low birth weight-though not mortality. This body of evidence has limitations that impede the formulation of firm conclusions, and new, well-focused studies are called for. The review findings suggest that continued strengthening of rules governing industrial emissions as well as industrial waste management and improved land use planning are needed.

[Brief Introduction of Pollution Sites Remediation and Risk Assessment and Its Policy Making in United States].

Site remediation has become an imperative part of environmental protection in China due to recent economic development, urban spreading, new industries replacing old ones, relocation of old industrial sites, and increased environmental conscience. This paper mainly introduced the concept, method, calculation, risk assessment and management for polluted sites remediation based on experience from California, USA. Further, the paper presented the concept of vapor intrusion and how to use vapor intrusion methodology to determine site remediation standard. Mathematical modeling approaches were also discussed in terms of how to determine the residual pollutant concentrations in soil and how to calculate indoor vapor concentrations. Based on risk assessment, California Environmental Protection Agency, Water Resources Control Board issued a 'Low Threat Underground Storage Tank Case Closure Policy' for impacted underground storage tank sites. The numerical criteria in the Policy were based on calculations of human health risk assessment. Finally, a real case study in California, USA, was presented to demonstrate how the risk assessment calculations were applied in polluted site remediation, which helps to answer the question of 'how clean is clean'.

Bioremediation of Southern Mediterranean oil polluted sites comes of age.

Mediterranean Sea is facing a very high risk of oil pollution due to the high number of oil extractive and refining sites along the basin coasts, and the intense maritime traffic of oil tankers. All the Mediterranean countries have adopted severe regulations for minimizing pollution events and bioremediation feasibility studies for the most urgent polluted sites are undergoing. However, the analysis of the scientific studies applying modern 'meta-omics' technologies that have been performed on marine oil pollution worldwide showed that the Southern Mediterranean side has been neglected by the international research. Most of the studies in the Mediterranean Sea have been done in polluted sites of the Northern side of the basin. Those of the Southern side are poorly studied, despite many of the Southern countries being major oil producers and exporters. The recently EU-funded research project ULIXES has as a major objective to increase the knowledge of the bioremediation potential of sites from the Southern Mediterranean countries. ULIXES is targeting four major polluted sites on the coastlines of Egypt, Jordan, Morocco and Tunisia, including seashore sands, lagoons, and oil refinery polluted sediments. The research is designed to unravel, categorize, catalogue, exploit and manage the diversity and ecology of microorganisms thriving in these polluted sites. Isolation of novel hydrocarbon degrading microbes and a series of state of the art 'meta-omics' technologies are the baseline tools for improving our knowledge on biodegradation capacities mediated by microbes under different environmental settings and for designing novel site-tailored bioremediation approaches. A network of twelve European and Southern Mediterranean partners is cooperating for plugging the existing gap of knowledge for the development of novel bioremediation processes targeting such poorly investigated polluted sites.