A cross-sectional study on occupational exposure to microorganisms, endotoxin, hydrogen sulfide, and dust during work at drilling waste treatment plants.

This cross-sectional study aims to obtain knowledge about workers' exposure to airborne dust, bacterial and fungal species, endotoxin, biofilm formation, and hydrogen sulfide (H2S) in drilling waste treatment plants. In total, 408 full-shift personal samples, 66 work areas, 40 drilling waste, and reference (outdoor air and seawater) samples were analyzed. Some workers were exposed to high levels of endotoxin (207 EU/m3), bacteria (3.8 × 104 colony forming units (CFU)/m3 and 9.8 × 104 DNA copies/m3), or fungi (1.4 × 107 CFU/m3 and 3,600 copies/m3). The exposure levels to endotoxin, bacteria, and peaks of H2S were dependent on the treatment technique. All types of drilling waste contained large concentrations of bacteria compared to the seawater references. Elevated concentrations of airborne bacteria were found close to drilling waste basins. In total, 116, 146, and 112 different bacterial species were found in workers' exposure, work areas, and the drilling waste, respectively. An overlap in bacterial species found in the drilling waste and air (personal and work area) samples was found. Of the bacterial species found, 49 are classified as human pathogens such as Escherichia coli, Enterobacter cloacae, and Klebsiella oxytoca. In total, 44 fungal species were found in the working environment, and 6 of these are classified as human pathogens such as Aspergillus fumigatus. In conclusion, across the drilling waste treatment plants, human pathogens were present in the drilling waste, and workers' exposure was affected by the drilling waste treated at the plants with elevated exposure to endotoxin and bacteria. Elevated exposure was related to working as apprentices or chemical engineers, and working with cleaning, or slop water, and working in the daytime.

Banana starch modified by heat moisture treatment and annealing: Study on digestion kinetics and enzyme affinity.

Starch modification by annealing (ANN) and heat-moisture treatment (HMT) results in a lower crystallinity compared to native but the change of B crystalline type to A type is only observed in HMT starch. All starches possess two different digestion rate constants i.e. k1 (at rapid phase) and k2 (at slow phase) which may be linked to the preserved intact starch granule following thermal treatment. HMT starch contains higher content of slowly digestible starch (C2∞) compared to the C2∞ of the other starches. The lower enzyme binding to HMT starch (Kd value increases from 0.12 mg/mL in native starch to 0.83 mg/mL) may be linked to the increase in the degree of ordered structure of the granule surface (observed from the absorption band ratio of 1000 cm-1/1022 cm-1). The lower affinity may lead to a lower k1 value. This holds true for ANN and native starch which displays similar k1, Kd value and degree of ordered to disordered structure. Lower k2 in HMT starch compared to the corresponding k2 in the other starches may be linked to the slower enzyme diffusion into the core of starch granule due to the tightly packed structure of A crystalline type in HMT starch.

Association between estimated glomerular filtration rate slope and cardiovascular disease among individuals with and without diabetes: a prospective cohort study.

BACKGROUND: Previous studies have reported an association between a significant decline in estimated glomerular filtration rate (eGFR) over time and an increased risk of cardiovascular disease (CVD). This study aimed to investigate the association between the eGFR slope and CVD among individuals with and without diabetes. METHODS: This prospective cohort study was conducted within the Tehran Lipid and Glucose Study (TLGS) framework. We studied 6919 adults aged 20-70 years, including 985 with diabetes and 5934 without diabetes. The eGFR slope was determined based on repeated measurements of eGFR through linear mixed-effects models. A multivariable Cox proportional hazard model was employed to evaluate the association between eGFR slope, both in continuous and categorical form, and the risk of CVD. RESULTS: The slopes of eGFR exhibited a bell-shaped distribution, with a mean (standard deviation (SD)) of -0.63 (0.13) and - 0.70 (0.14) ml/min per 1.73 m2 per year in individuals with and without diabetes, respectively. During a median follow-up of 8.22 years, following the 9-year eGFR slope ascertainment period, a total of 551 CVD events (195 in patients with diabetes) were observed. Among individuals with diabetes, a steeper decline in eGFR slope was significantly associated with a higher risk of CVD events, even after adjusting for baseline eGFR, demographic factors, and traditional risk factors for CVD; slopes of (-1.05 to -0.74) and (-0.60 to -0.52) were associated with 2.12 and %64 higher risks for CVD, respectively, compared with a slope of (-0.51 to 0.16). Among individuals without diabetes, the annual eGFR slope did not show a significant association with the risk of CVD. CONCLUSION: Monitoring the eGFR slope may serve as a potential predictor of CVD risk in individuals with diabetes.

Trend Analysis of Gallbladder Cancer for Dibrugarh District, Assam, During the Period of 2003-2016.

The incidence of gallbladder cancer (GBC) is very high in India and it increases day by day due to population aging and changing of life style. The current study scrutinized the trend analysis of gallbladder cancer of Dibrugarh district. The registered gallbladder cancer data for the period of 14 years, i.e., 2003-2016, is collected from Population-Based Cancer Registry (PBCR), Upper Assam. The Mann Kendall and Sen's Slop test is performed to analyze the gallbladder cancer data. A total of 716 number of patients with gallbladder cancer were included and overall gallbladder cancer was found highest among patients of age 50-70 years, accounting for 56.84% (n = 407). The disease was most common in females (69.13%) in comparison to males (30.87%) and most of the patients were from urban areas (66.89%). Majority (29.19%) of the patients had completed their primary education whereas 22.07% were illiterate. Mean of age at the time of diagnosis was 54.51 ± 12.29 years which was 57.43 ± 12.19 for male and 53.21 ± 12.13 for female. The time trends and patterns of gallbladder cancer have striking differences within the country as well as in the state of Assam. Large, focussed, inclusive studies are required in India to assess early detection and diagnosis of gallbladder cancer in the country.

Exceptional adsorption of different spectral indices of natural organic matter (NOM) by using cerium oxide nanoparticles (CONPs).

In this study, the efficiency of cerium oxide nanoparticles (CONPs) was examined for the adsorptive removal of various spectral indices of Natural Organic Matter (NOM). Two methods, viz. efficient microwave combustion (ECM) and hydroxide mediated approach (HMA), were used to synthesize CONPs. The developed materials were characterized by the field emission scanning electron microscope (FESEM) with energy dispersive X-ray (EDX) and the Fourier transform infrared spectroscopy (FTIR). Moreover, the X-ray powder diffraction (XRD) confirmed the cubic structure with an average crystal size of 20.16 nm (CONP-I) and 6.75 nm (CONP-II). The observed point of zero (pHPZC) charge was approximately 7.0. The enhanced BET surface area (85.43 m2/g, 78.59 m2/g) and pore volume (0.007310 cm3/g, 0.006761 cm3/g) of CONPs support the higher adsorption. The effect of operational parameters (pH, contact time, and adsorbent dosage) and thermodynamical aspects of adsorption was also investigated. The Temkin isotherms described the experimental data better, with a maximum adsorption capacity of 238.9 mg/g (CONP-I) at neutral pH. Further, the experimental data can better be modeled by the pseudo-second-order kinetics (R2, 0.9851). Overall, CONPs possess great efficiency for the simultaneous removal of DOC (94%), UV254 (93%), adsorption slop index (ASI) (95%), phenolic content (88%), and carboxylic content (73%).

The Escherichia coli CitT transporter can be used as a succinate exporter for succinate production.

Escherichia coli strain, whose gene is one of the subunits of succinate dehydrogenase (sdhA), and gene of the transcriptional repressor of isocitrate lyase (iclR) were disrupted, accumulated 6.6 times as much intracellular succinate as the wild-type MG1655 strain in aerobic growth, but succinate was not found in the culture medium. E. coli citT gene that encodes a citrate transporter was cloned under the control of the lacI promoter in pBR322-based plasmid and the above strain was transformed. This transformant, grown under aerobic condition in M9-tryptone medium with citrate, accumulated succinate in the medium while no succinate was found in the medium without citrate. CitT was active as a succinate transporter for 168 h by changing the culture medium or for 24 h in fed-batch culture. This study suggests that the CitT transporter functions as a succinate exporter in E. coli for succinate production in the presence of citrate.

Biodegradation of slop oil by endophytic Bacillus cereus EN18 coupled with lipase from Rhizomucor miehei (Palatase®).

Removal of slop oil, a by-product of oil refining, also obtained in cleaning up of oil tanks and filters is a difficult issue. High content of hydrocarbons (C3-C40) and other organic compounds makes this waste difficult to eliminate from the environment. The purpose of this investigation was to combine bacterial degradation by endophytic Bacillus cereus EN18 with biotransformation performed using lipase enzyme preparation (Palatase®) to remove recalcitrant compounds present in slop oil from the environment. Endophytic B. cereus EN18 was able to biodegrade up to 40% of slop oil while supplementation with lipase improved the efficiency of contamination removal in about one third. Also the use of lipase enzyme preparation resulted in higher microbial activity of B. cereus EN18 bacterial strain, as well as higher concentration of fatty acids in the culture medium, which indicates higher degradation efficiency. Obtained results suggest that lipase preparation from Rhizomucor miehei (Palatase®) may be a useful agent to improve microbial degradation of recalcitrant pollutants, like slop oil in water environments. GC and spectrometric analysis revealed that hydrocarbons from slop oil were effectively degraded while using both microbial degradation and lipase catalysis.

[C:N:P stoichiometry characteristics of litter and soil of forests in Great Xing'an Mountains with different fire years.] / å¤§å ´å®‰å²­ä¸åŒç«çƒ§å¹´é™æ£®æž—å‡‹è½ç‰©å’ŒåœŸå£¤C、N、P化学计量特征.

We investigated the fire impacts on nutrients in litter and soil, and their C:N:P stoichio-metry in forests of Great Xing'an Mountains. The studied sites differed in their burning year (post-fire 4, 14, 40, 70 years and unburned within 120 years) and had different topographic locations (sloped land and flat land). The results showed that there were significant differences in stoichio-metry characteristics of C, N, P for both litter and soil with different burning years. No significant fluctuation was observed for the litter C content, while the contents of litter N and P increased with the increasing post-fire recovery years. In specific, we found the contents of litter N and P decreased at post-fire 4 and 14 years and nearly recovered to the control level at 40 years after fire. Additionally, C:N and C:P ratios of litter decreased, but N:P ratio of litter increased following post-fire recovery time. The contents of C, N, P and their ratios (C:N, C:P and N:P) in soil decreased with soil depth. Soil C content exhibited an increasing trend following post-fire recovery time and was significantly higher than the control at post-fire 70 years in sloped land, but no significant difference in the flat land. Significant interactive effects between fire history and slope were observed in soil P content and C:P ratio. Soil P content was higher than the control at post-fire 4 years in sloped land, but was higher than the control at post-fire 40 years in flat land. The C:P ratio recovered to the control level at post-fire 14 years in sloped land, and there was no significant diffe-rence in flat land. Redundancy analysis showed that slope effect played a more vital role than fire history effect in soil organic layer, while fire history effect was the most important factor for the varia-tion of soil nutrients in soil mineral layer. In our study, nutrients of litter and soil were lower than the control level at post-fire 4 and 14 years. The quality of litter and soil was improved with accele-rated plant growth and litter decomposition following post-fire recovery time and recovered to the pre-fire level at post-fire 40 years, reaching a steady status.

Influences of nutrition and adiposity on bone mineral density in individuals with chronic spinal cord injury: A cross-sectional, observational study.

BACKGROUND: Dietary inadequacy and adiposity, both prevalent in the chronic spinal cord injury (SCI) population, are known to influence bone turnover and may be potential modifiable risk factors for the development of sublesional osteoporosis following SCI. This pilot study in an SCI cohort aimed to assess measures of nutrition and obesity, to determine if these measures were associated with bone mineral density (BMD), and to compare these measures to a non-SCI control cohort. METHODS: In a cross-sectional observational study, volunteers with chronic SCI (> 1 year post-injury, lesions from C1 to T12 and severity category A-D by the American Spinal Injury Association Impairment Scale) were assessed, and 8 non-SCI individuals were recruited as a comparison group. BMD at the femoral neck (FN) and lumbar spine (LS), and an estimate of visceral adipose tissue (VAT) from lumbar vertebrae 1 through 4 were measured using dual energy X-ray absorptiometry (DXA); nutrient intake of calcium, vitamins D & K, and protein were estimated using a food frequency questionnaire; plasma 25-hydroxyvitamin D (25(OH)D) was analyzed using ultra-high performance liquid chromatography/tandem mass spectroscopy; and serum leptin, adiponectin and insulin were analyzed using a multiplex assay. RESULTS: A total of 34 individuals with SCI (n = 22 tetraplegic; n = 12 paraplegic; 94% male) who averaged 12.7 (9.0) years post-injury, age 40.0 (10.9) years and % body fat of 28.4 (7.3) were assessed. Multiple linear regression analyses in the SCI cohort showed significant associations between BMD at the FN and LS with leptin (FN: r = 0.529, p = 0.005; LS: r = 0.392, p = 0.05), insulin (FN: r = 0.544, p = 0.003; LS: r = 0.388, p = 0.05), and VAT percent (FN: r = 0.444, p = 0.02; LS: r = 0.381, p = 0.05). Adiponectin was only correlated with LS BMD (r = 0.429, p = 0.03). No significant relationships were found between BMD and serum 25(OH)D, or intakes of calcium, vitamins D & K, and protein. Intake of vitamin D was adequate in 69% of participants with SCI, where 91% of those persons consumed either vitamin D and/or multivitamin supplements. Vitamin D status was similar between SCI and non-SCI groups as was sub-optimal status (25(OH)D < 75 nmol/L) (60% of SCI compared to 50% of non-SCI). Participants with SCI had significantly lower FN BMD in comparison to non-SCI controls (p = 0.001). CONCLUSIONS: Compromised BMD among individuals with SCI was not associated with a deficiency of vitamin D or other bone nutrients. The observed positive associations between BMD and leptin, insulin, adiponectin and VAT provide a framework to evaluate links between adiposity and bone health in a larger SCI cohort.