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In central Canada, surveys for the three invasive (Palearctic) Agriotes species-A. obscurus, A. lineatus, and A. sputator-commonly collect A. pubescens, a North American native (Nearctic) click beetle (Coleoptera: Elateridae) that generally co-occurs with Nearctic A. mancus. Despite the abundance of A. pubescens on farmland, its life history and potential economic impact remain largely unknown. Here, we report the identification and field testing of the A. pubescens sex pheromone. We collected headspace volatiles from a single female beetle on Porapak Q, then extracted the female's pheromone gland, and analyzed aliquots of both Porapak extract and pheromone gland extract by gas chromatographic-electroantennographic detection (GC-EAD) and by GC-mass spectrometry. In GC-EAD recordings of gland extract, two esters-geranyl butanoate and geranyl octanoate-elicited antennal responses from A. pubescens males. In a field experiment in Quebec, traps baited with a 1:1 blend of these esters captured approximately 100× more A. pubescens males than traps baited with a single ester. This is the same trap lure used for capturing A. lineatus. In this experiment, and in a similar one run in British Columbia, heterospecific pheromone components added to conspecific pheromone lures reduced captures of A. mancus, A. sputator, A. lineatus, and A. obscurus by 29%, 96%, 44%, and 71%, respectively. These data indicate that, in North America, lures containing pheromone components of multiple Agriotes congeners may not be optimally attractive to all target species.
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Current understanding of atmospheric transport of semivolatile organic contaminants (SVOCs) in alpine areas is limited due to complex meteorology and topography. Salt Lake City, Utah borders protected wilderness areas in the Wasatch Mountains, exhibiting a useful model system in which an urban source of SVOCs, including polyaromatic hydrocarbons (PAHs) and polychlorinated biphenyls (PCBs), is located directly adjacent to an alpine sink. Our objective was to investigate the impacts of topographical features on the transport and deposition of SVOCs across an urban-alpine boundary. To do so, we measured PAHs and PCBs in soils along a transect starting at the urban-mountain interface and extending into an alpine wilderness, crossing several prominent ridgelines. Concentrations of PAHs and PCBs in soils were heavily influenced by soil organic carbon content, air temperature, and proximity to the urban boundary. However, the role of source proximity was only revealed after normalizing concentrations in soil to organic carbon content and air temperature. Further, we present evidence of SVOC emission/deposition cycles driven by diurnal alpine winds that do not extend past topographical features. Our results illustrate the roles of multiple competing processes on SVOC transport in alpine systems and their importance at an urban-alpine boundary.
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Poluentes Atmosféricos , Atmosfera , Bifenilos Policlorados , Atmosfera/química , Poluentes Atmosféricos/análise , Monitoramento Ambiental , Hidrocarbonetos Policíclicos Aromáticos/análise , Cidades , Compostos Orgânicos Voláteis , Solo/química , Poluentes do Solo , UtahRESUMO
The coronavirus disease 2019 (COVID-19) pandemic, caused by the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), continues to challenge global health despite widespread vaccination efforts, underscoring the need for innovative strategies to combat emerging infectious diseases effectively. Herein, LCB1-NPs and LCB3-NPs are engineered as a novel class of protein-only nanoparticles formed through coiled coil-driven self-assembly and tailored to interact specifically with the SARS-CoV-2 spike protein. The multivalency of LCB1-NPs and LCB3-NPs offers a strategy for efficiently targeting and neutralizing SARS-CoV-2 both in solution and when immobilized on surfaces. It is demonstrated that LCB1-NPs and LCB3-NPs bind to the SARS-CoV-2 spike protein's receptor-binding domain (RBD) with high affinity, effectively blocking the entry of SARS-CoV-2 virus-like particles into angiotensin-converting enzyme 2 (ACE2)-coated human cells. The cost-effectiveness, scalability, and straightforward production process of these protein nanoparticles make them suitable for developing novel anti-viral materials. Accordingly, it is shown how these nanostructures can be packed into columns to build up economic and highly potent trapping devices for SARS-CoV-2 adsorption.
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Radical-based advanced oxidation processes (AOPs) are among the most effective technologies employed to destroy organic pollutants. Compared to common inorganic radicals, such as â¢OH, O2â¢-, and SO4â¢-, organic radicals are widespread, and more selective, but are easily overlooked. Furthermore, a systematic understanding of the generation and contributions of organic radicals remains lacking. In this review, we systematically summarize the properties, possible generation pathways, detection methods, and contributions of organic radicals in AOPs. Notably, exploring organic radicals in AOPs is challenging due to (1) limited detection methods for generated organic radicals; (2) controversial organic radical-mediated reaction mechanisms; and (3) rapid transformation of organic radicals as reaction intermediates. In addition to their characteristics and reactivity, we examine potential scenarios of organic radical generation in AOPs, including during the peroxide activation process, in water matrices or with coexisting organic pollutants, and due to the addition of quenching agents. Subsequently, we summarize various methods for organic radical detection as reported previously, such as electron paramagnetic resonance spectroscopy (EPR), 31P nuclear magnetic resonance spectroscopy (31P NMR), liquid/gas chromatography-mass spectroscopy (GC/LC-MS), and fluorescence probes. Finally, we review the contributions of organic radicals to decontamination processes and provide recommendations for future research.
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Background: Chronic obstructive pulmonary disease (COPD) often presents with dyspnea resulting from the condition of air trapping, assessed by lung volume measurement studies. This study aimed to investigate the relationship between handgrip strength (HGS) and air trapping in COPD patients. Methods: Cross-sectional research was conducted in COPD patients at Thammasat University Hospital, Thailand between May 2022 and December 2023. HGS was assessed using the Jamar® Smart Hand Dynamometer, and air trapping was measured using a body plethysmograph. Air trapping was defined as a ratio of residual volume (RV) to total lung capacity (TLC) greater than 40%. Receiver operator characteristic (ROC) curves, sensitivity, and specificity values were calculated to determine the optimal cutoff value of HGS for predicting air trapping. Results: A total of 72 patients (90.3% male) were included, with an average age of 72.4±9.7 years. The body mass index was 23.5±4.3 kg/m2. The smoking history was 23.2±14.8 pack-years. Common comorbidities included hypertension (36.1%) and diabetes (22.2%). Post-bronchodilator forced expiratory volume in 1 second (FEV1) was 72.1%±21.2%. Air trapping was found in 55.6%. A negative correlation was found between HGS and RV/TLC (R=-0.399, P=0.001). The best cutoff value for HGS to predict air trapping was 28.3 kg, with 71.9% sensitivity and 65.0% specificity. The area under the ROC curve for identifying air trapping was 0.681 (95% CI: 0.554 to 0.808, P=0.009). Conclusions: Air trapping is common in COPD patients, and HGS is significantly correlated with air trapping. Thus, HGS may serve as an alternative tool for assessing air trapping.
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Myosins are ATP-powered, force-generating motor proteins involved in cardiac and muscle contraction. The external load experienced by the myosins modulates and coordinates their function in vivo. Here, this study investigates the tension-sensing mechanisms of rabbit native ß-cardiac myosin (ßM-II) and slow skeletal myosins (SolM-II) that perform in different physiological settings. Using mobile optical tweezers with a square wave-scanning mode, a range of external assisting and resisting loads from 0 to 15 pN is exerted on single myosin molecules as they interact with the actin filament. Influenced of load on specific strongly-bound states in the cross-bridge cycle is examined by adjusting the [ATP]. The results implies that the detachment kinetics of actomyosin ADP.Pi strongly-bound force-generating state are load sensitive. Low assisting load accelerates, while the resisting load hinders the actomyosin detachment, presumably, by slowing both the Pi and ADP release. However, under both high assisting and resisting load, the rate of actomyosin dissociation decelerates. The transition from actomyosin ADP.Pi to ADP state appears to occur with a higher probability for ßM-II than SolM-II. This study interpret that dissociation of at least three strongly-bound actomyosin states are load-sensitive and may contribute to functional diversity among different myosins.
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Hibiscus mealybug, Nipaecoccus viridis (Newstead) (Hemiptera: Pseudococcidae), is a recent invasive pest of citrus and many other crops in Florida. Nipaecoccus viridis attacks all above ground parts of citrus trees and heavy infestation can cause leaf drop and premature abortion of developing fruits. We quantified greater captures of N. viridis in cardboard band traps on areas of citrus trees that were intentionally injured by mechanical rasping of epidermal tissues as compared with similar but uninjured citrus branches. Direct field collection of headspace volatiles from mechanically injured or intact citrus branches revealed both qualitative and quantitative differences. Certain volatiles (γ-terpinene, citronellal, citronellyl acetate, ß-E-farnesene, α-humulene, and α-E-E-farnesene) were only present in samples from damaged citrus branches. Behavioral assays using a laboratory Y-tube olfactometer revealed attraction of N. viridis to volatiles associated with mechanical damage of citrus including synthetic ß-ocimene, γ-terpinene, sabinene, isomers of farnesene, and citronellal when loaded into lures at either of 2 concentrations (0.01 or 0.1â µg/µl). Subsequent field trapping experiments confirmed increased captures of various life stages of N. viridis in cardboard band traps baited with a 10.0â µg/µl concentration of farnesene:ocimene:sabinene blend (in 7:13:17 ratio), as well as those releasing either farnesene or ocimene alone at this same concentration, as compared with the mineral oil (diluent) negative control. Our results indicate that common plant related terpenes released by citrus following mechanical damage may be useful for development of an effective monitoring trap for N. viridis.
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Distal anterior cerebral artery (ACA) aneurysms comprise 4-5% of all intracranial aneurysms.1-3 Rarely, these aneurysms can be complex and less amenable to conventional clipping or endovascular techniques, requiring alternative treatment strategies.4,5 Surgical modalities employed in these situations may involve trapping and flow replacement techniques to exclude the aneurysm while maintaining normal perfusion to the affected territories.4-7 In this video, we describe the Modified Trapping Technique for cases where two branches arise from the aneurysm and cannot be sacrificed. This technique involves the transposition of one of the branches and its reimplantation distally to the lesion. The aneurysm is then clipped, trapping the segment from which the disconnected branch originated, while preserving anterograde blood flow to both non-occluded and reimplanted branches. Compared to complete trapping in similar situations, this technique prevents the formation of a dead-end in the parent artery that could lead to thrombosis,8 poses no risk to uninvolved arteries and requires only one anastomosis. This technique was applied in a 54-year-old female patient who presented at our institution with an incidental fusiform distal ACA aneurysm diagnosed after head trauma. Imaging demonstrated that the aneurysm originated from a bihemispheric ACA with two pericallosal arteries arising from it. The patient tolerated the procedure well, and postoperative imaging showed complete aneurysm occlusion and patency of both the non-occluded and reimplanted pericallosal arteries. The patient consented to the procedure and the publication of her images. Institutional review board approval was deemed unnecessary.
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Electrochemical impedance spectroscopy (EIS) is a non-invasive and label-free method widely used for characterizing cell cultures and monitoring their structure, behavior, proliferation and viability. Microfluidic systems are often used in combination with EIS methods utilizing small dimensions, controllable physicochemical microenvironments and offering rapid real-time measurements. In this work, an electrode array capable of conducting EIS measurements was integrated into a multichannel microfluidic chip which is able to trap individual cells or cell populations in specially designed channels comparable to the size of cells. An application-specific printed circuit board (PCB) was designed for the implementation of the impedance measurement in order to facilitate connection with the device used for taking EIS spectra and for selecting the channels to be measured. The PCB was designed in consideration of the optical screening of trapped cells in parallel with the EIS measurements which allows the comparison of EIS data with optical signals. With continuous EIS measurement, the filling of channels with cell suspension can be followed. Yeast cells were trapped in the microfluidic system and EIS spectra were recorded considering each individual channel, which allows differentiating between the number of trapped cells.
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Espectroscopia Dielétrica , Espectroscopia Dielétrica/métodos , Espectroscopia Dielétrica/instrumentação , Técnicas Analíticas Microfluídicas/instrumentação , Técnicas Analíticas Microfluídicas/métodos , Saccharomyces cerevisiae/citologia , Microfluídica/métodos , Microfluídica/instrumentação , Eletrodos , Técnicas Eletroquímicas/métodos , Técnicas Eletroquímicas/instrumentaçãoRESUMO
Changes in lunar illumination alter the balance of risks and opportunities for animals, influencing activity patterns and species interactions. We examined if and how terrestrial mammals respond to the lunar cycle in some of the darkest places: the floors of tropical forests. We analysed long-term camera trapping data on 86 mammal species from 17 protected forests on three continents. Conservative categorization of activity during the night revealed pronounced avoidance of moonlight (lunar phobia) in 12 species, compared with pronounced attraction to moonlight (lunar philia) in only three species. However, half of all species in our study responded to lunar phases, either changing how nocturnal they were, altering their overall level of activity, or both. Avoidance of full moon was more common, exhibited by 30% of all species compared with 20% of species that exhibited attraction. Nocturnal species, especially rodents, were over-represented among species that avoided full moon. Artiodactyla were more prominent among species attracted to full moon. Our findings indicate that lunar phases influence animal behaviour even beneath the forest canopy. Such impacts may be exacerbated in degraded and fragmented forests. Our study offers a baseline representing relatively intact and well-protected contexts together with an intuitive approach for detecting activity shifts in response to environmental change.
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Florestas , Mamíferos , Lua , Animais , Mamíferos/fisiologia , Clima Tropical , Comportamento AnimalRESUMO
Porous organic cages (POCs) with permanent porosity and excellent host-guest property hold great potentials in regulating ion transport behavior, yet their feasibility as solid-state electrolytes has never been testified in a practical battery. Herein, we design and fabricate a quasi-solid-state electrolyte (QSSE) based on a POC to enable the stable operation of Li-metal batteries (LMBs). Benefiting from the ordered channels and cavity-induced anion-trapping effect of POC, the resulting POC-based QSSE exhibits a high Li+ transference number of 0.67 and a high ionic conductivity of 1.25 × 10-4 S cm-1 with a low activation energy of 0.17 eV. These allow for homogeneous Li deposition and highly reversible Li plating/stripping for over 2000 h. As a proof of concept, the LMB assembled with POC-based QSSE demonstrates extremely stable cycling performance with 85% capacity retention after 1000 cycles. Therefore, our work demonstrates the practical applicability of POC as SSEs for LMBs and could be extended to other energy-storage systems, such as Na and K batteries.
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We aimed to fabricate reliable memory devices using HfO2, which is gaining attention as a charge-trapping layer material for next-generation NAND flash memory. To this end, a new atomic layer deposition process using sequential remote plasma (RP) and direct plasma (DP) was designed to create charge-trapping memory devices. Subsequently, the operational characteristics of the devices were analyzed based on the thickness ratio of thin films deposited using the sequential RP and DP processes. As the thickness of the initially RP-deposited thin film increased, the memory window and retention also increased, while the interface defect density and leakage current decreased. When the thickness of the RP-deposited thin film was 7 nm, a maximum memory window of 10.1 V was achieved at an operating voltage of ±10 V, and the interface trap density (Dit) reached a minimum value of 1.0 × 1012 eV-1cm-2. Once the RP-deposited thin film reaches a certain thickness, the ion bombardment effect from DP on the substrate is expected to decrease, improving the Si/SiO2/HfO2 interface and thereby enhancing device endurance and reliability. This study confirmed that the proposed sequential RP and DP deposition processes could resolve issues related to unstable interface layers, improve device performance, and enhance process throughput.
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Alzheimer's disease (AD) is a debilitating neurodegenerative disorder characterized by brain aggregation of ß-amyloid (Aß) peptides and phosphorylated tau (P-tau) proteins. Extracellular vesicles (EVs) can be isolated and studied for potential roles in disease. While several studies have tested plasma-derived EVs in AD, few have analyzed EVs from cerebrospinal fluid (CSF), which are potentially more closely related to brain changes. This study included 20 AD patients and 20 cognitively unimpaired (CU) participants. Using a novel EV isolation method based on acoustic trapping, we isolated and purified EVs from minimal CSF volumes. EVs were lysed and analyzed by immunoassays for P-tau217 and P-tau181. Isolation was confirmed through transmission electron microscopy and the presence of EV-specific markers (CD9, CD63, CD81, ATP1A3). Nanoparticle tracking analysis revealed a high variance in EV distribution. AD patients exhibited increased P-tau181 and decreased P-tau217 in EVs, leading to a higher EV P-tau181/P-tau217 ratio compared to CU. No significant differences in EV counts or sizes were observed between AD and CU groups. This study is the first to use acoustic trapping to isolate EVs from CSF and demonstrates differential P-tau content in AD-derived EVs, warranting further research to understand the relationship between these EV changes and brain pathology.
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Doença de Alzheimer , Vesículas Extracelulares , Proteínas tau , Idoso , Idoso de 80 Anos ou mais , Feminino , Humanos , Masculino , Pessoa de Meia-Idade , Doença de Alzheimer/líquido cefalorraquidiano , Doença de Alzheimer/metabolismo , Doença de Alzheimer/patologia , Biomarcadores/líquido cefalorraquidiano , Vesículas Extracelulares/metabolismo , Fosforilação , Projetos Piloto , Proteínas tau/líquido cefalorraquidiano , Proteínas tau/metabolismoRESUMO
The past decade has witnessed remarkable progress in high-performance Triboelectric nanogenerators (TENG) with the design and synthesis of functional dielectric materials, the exploration of novel dynamic charge transport mechanisms, and the innovative design of architecture, making it one of the most crucial technologies for energy harvesting. High output charge density is fundamental for TENG to expand its application scope and accelerate industrialization; it depends on the dynamic equilibrium of charge generation, trapping, de-trapping, and migration within its core components. Here, this review classifies and summarizes innovative approaches to enhance the charge density of the charge generation, charge trapping, and charge collection layers. The milestone of high charge density TENG is reviewed based on material selection and innovative mechanisms. The state-of-the-art principles and techniques for generating high charge density and suppressing charge decay are discussed and highlighted in detail, and the distinct charge transport mechanisms, the technologies of advanced materials preparation, and the effective charge excitation strategy are emphatically introduced. Lastly, the bottleneck and future research priorities for boosting the output charge density are summarized. A summary of these cutting-edge developments intends to provide readers with a deep understanding of the future design of high-output TENG.
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Diocalandra frumenti (Fabricius) (Coleoptera: Dryophthoridae) is a weevil present in the Canary Islands, affecting economically important palms such as Phoenix canariensis H. Wildpret and its hybrids, for which there were no trapping tools. The larvae cause the main damage by burrowing galleries in the rachis of the leaves, causing premature drying and collapse. To develop an effective trap, six trials were carried out to evaluate the effect of trap type, design, colour, height, distance and location of the trap in relation to the palm tree on D. frumenti captures. This study confirms that the Econex® trap, green in colour, without a cover and with two ventilation holes of 2.5 cm in diameter, diametrically opposite each other and at 1 cm from the top of the base of the trap, baited with sugar cane and water, and placed between the first and second ring of green leaves of the palm canopy, is efficient in capturing D. frumenti. These results establish a basis for future research focused on the development of a specific trapping system based on semiochemicals to serve as a tool for detection, monitoring and mass trapping of D. frumenti.
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Halyomorpha halys (Stål) (Hemiptera: Pentatomidae) is an invasive pest causing significant damage to tree crops. Our study examined the impact of newly designed aggregation pheromone-baited 'mini-sailboat' (MSB) traps for controlling H. halys and its effect on the spatial distribution of fruit damage. Four replicates of four traps, with a total of 16 MSB traps, were placed along a 1.3 km border of apple orchards, concentrating the traps on one side of the orchards. A fruit damage assessment for incidence and severity was conducted at increasing distances from the orchard border where the traps were placed, encompassing 107 assessment points. Our study showed that deploying MSB traps along the orchard border significantly increased fruit damage within the first 45 m compared to control plots without traps. However, beyond the first 45 m from the border, there was a significant reduction in damage incidence. In the treated plots, 50% of the damage occurred within 26 m of the traps, while in control plots, within 85 m. Shifting the fruit damage pattern means restricting the pests lingering in a narrow strip near the MSB traps, which paves the way for improved techniques to restructure the crop perimeter.
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Tungsten diselenide (WSe2) field-effect transistors (FETs) are promising for emerging electronics because of their tunable polarity, enabling complementary transistor technology, and their suitability for flexible electronics through material transfer. In this work, we demonstrate flexible p-type WSe2 FETs with absolute drain currents |ID| up to 7 µA/µm. We achieve this by fabricating flexible top-gated FETs with a combined WSe2 and metal contact transfer approach using WSe2 grown by metal-organic chemical vapor deposition on sapphire. Despite moderate WSe2 crystal grain size, our devices show similar or higher |ID| and ID on/off ratio (â¼105) compared to most devices with exfoliated single-crystal WSe2 from the literature. We analyze charge trapping in our devices using pulsed and bias stress measurements. Notably, the high |ID| values are preserved during pulsing, where charge trapping is minimized. Overall, we demonstrate a fabrication approach advantageous for high drain currents in flexible 2D transistors.
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Previously developed behavioral approaches for controlling the apple maggot fly, Rhagoletis pomonella (Walsh) (Diptera: Tephritidae), include attract-and-kill (AK) systems such as perimeter trapping using either, odor-baited red sticky spheres or odor-baited, sticky-free attracticidal spheres with contoured tops that ensure the sustained release of both insecticide and feeding stimulant. Here, over a 3-year span in 26 commercial apple orchards across Massachusetts, New Hampshire, and Maine, we evaluated the effectiveness of a novel AK strategy for R. pomonella management. Synthetic apple blend lures were deployed on perimeter-row trees to attract the pest, while perimeter-row insecticide sprays blended with 2% sugar, a known phagostimulant, served as the lethal component. Comparatively, grower standard (GS) blocks received full-block insecticide sprays devoid of sugar and lures. Monitoring revealed that red sticky spheres in AK blocks captured significantly more R. pomonella adults than unbaited monitoring spheres in GS blocks. Penetration of R. pomonella into block interiors remained consistently low and statistically similar for both AK and GS blocks. Whole-block infestation levels showed no significant difference between the 2 management approaches. Notably, insecticide application in AK blocks was reduced by 75%, 64.7%, and 64.2% in 2019, 2020, and 2021, respectively, compared with GS blocks. This grower-friendly AK approach could be integral to a reduced insecticide spray-based strategy for apple cultivation in the northeastern United States.
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Introductions of transboundary animal diseases (TADs) into free-ranging wildlife can be difficult to control and devastating for domestic livestock trade. Combating a new TAD introduction in wildlife with an emergency response requires quickly limiting spread of the disease by intensely removing wild animals within a contiguous area. In the case of African swine fever virus (ASFv) in wild pigs (Sus scrofa), which has been spreading in many regions of the world, there is little information on the time- and cost-efficiency of methods for intensively and consistently culling wild pigs and recovering carcasses in an emergency response scenario. We compared the efficiencies of aerial operations, trapping, experimental toxic baiting, and ground shooting in northcentral Texas, USA during two months in 2023. Culling and recovering carcasses of wild pigs averaged a rate of 0.15 wild pigs/person hour and cost an average of $233.04/wild pig ($USD 2023) across all four methods. Aerial operations required the greatest initial investment but subsequently was the most time- and cost-efficient, costing an average of $7266 to reduce the population by a standard measure of 10â¯%, including recovering carcasses. Aerial operations required a ground crew of â¼7 people/helicopter to recover carcasses. Costs for reducing the population of wild pigs using trapping were similar, although took 13.5 times longer to accomplish. In cases where carcass recovery and disposal are needed (e.g., response to ASFv), a benefit of trapping was immediate carcass recovery. Toxic baiting was less efficient because both culling and carcass recovery required substantial time. We culled very few wild pigs with ground shooting in this landscape. Our results provide insight on the efficiencies of each removal method. Strategically combining removal methods may increase overall efficiency. Overall, our findings inform the preparation of resources, personnel needs, and deployment readiness for TAD responses involving wild pigs.
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Surface modification, including the anchoring of functional groups is a popular method to increase the photocatalytic activity of semiconductor photocatalysts. These species can trap excited electrons, thus prolonging the life of the charge carriers. N-containing functional groups are suitable for this purpose due to their high electron density. Here, we report a facile synthesis method for preparing interfacial N-based functional groups-modified and nitrogen-doped SrTiO3 photocatalysts. Among the modified samples (with 0.42-11.14 at.% nominal nitrogen content), the one with 7.71 at.% nitrogen showed 6.4 times higher photooxidation efficiency for phenol and 2.2 times better photoreduction efficiency for CO2 conversion than the unmodified SrTiO3 reference. Characterization results showed that using a low amount of nitrogen source resulted in low but measurable nitrogen doping, which did not significantly affect the photocatalytic activity. The formation of surface amine groups was significant even at lower initial nitrogen concentrations, while higher amounts of nitrogen source gradually resulted in the incorporation of nitrogen in higher amounts. Surface amine groups decreased the recombination of charge carriers, resulting in increased photocatalytic activity.