RESUMO
Mitochondrial aconitase is a reversible enzyme that catalyzes the conversion of citrate to isocitrate in the tricarboxylic acid cycle. Mitochondrial aconitase is very sensitive to oxidative inactivation and can aggregate and accumulate in the mitochondrial matrix causing mitochondrial dysfunction. Lon protease, one of the major quality control proteases in mitochondria, degrades oxidized aconitase maintaining mitochondrial homeostasis. This chapter describes a step-by-step protocol for a simple and reliable measurement of mitochondrial aconitase, as well as citrate synthase activity, using isolated mitochondria from cells. The protocol is simple and fast, and it is optimized for a 96-well plate using a microplate reader.
Assuntos
Proteases Dependentes de ATP/metabolismo , Aconitato Hidratase/análise , Ensaios Enzimáticos/métodos , Proteínas Mitocondriais/metabolismo , Aconitato Hidratase/metabolismo , Animais , Linhagem Celular Tumoral , Citrato (si)-Sintase/análise , Citrato (si)-Sintase/metabolismo , Ensaios Enzimáticos/instrumentação , Fibroblastos , Camundongos , Mitocôndrias/metabolismo , Oxirredução , Estresse Oxidativo , Cultura Primária de CélulasRESUMO
Candida albicans is an opportunist pathogen responsible for a large spectrum of infections, from superficial mycosis to the systemic disease candidiasis. Its ability to adopt various morphological forms, such as unicellular yeasts, filamentous pseudohyphae and hyphae, contributes to its ability to survive within the host. It has been suggested that the antioxidant glutathione is involved in the filamentation process. We investigated S-glutathionylation, the reversible binding of glutathione to proteins, and the functional consequences on C. albicans metabolic remodeling during the yeast-to-hyphae transition. Our work provided evidence for the specific glutathionylation of mitochondrial proteins involved in bioenergetics pathways in filamentous forms and a regulation of the main enzyme of the glyoxylate cycle, isocitrate lyase, by glutathionylation. Isocitrate lyase inactivation in the hyphal forms was reversed by glutaredoxin treatment, in agreement with a glutathionylation process, which was confirmed by proteomic data showing the binding of one glutathione molecule to the enzyme (data are available via ProteomeXchange with identifier PXD003685). We also assessed the effect of alternative carbon sources on glutathione levels and isocitrate lyase activity. Changes in nutrient availability led to morphological flexibility and were related to perturbations in glutathione levels and isocitrate lyase activity, confirming the key role of the maintenance of intracellular redox status in the adaptive metabolic strategy of the pathogen.
Assuntos
Candida albicans/crescimento & desenvolvimento , Candidíase/microbiologia , Proteínas Fúngicas/metabolismo , Glutationa/metabolismo , Hifas/crescimento & desenvolvimento , Proteínas Mitocondriais/metabolismo , Aconitato Hidratase/análise , Aconitato Hidratase/metabolismo , Sequência de Aminoácidos , Candida albicans/química , Candida albicans/enzimologia , Candida albicans/metabolismo , Proteínas Fúngicas/análise , Humanos , Hifas/química , Hifas/enzimologia , Hifas/metabolismo , Isocitrato Liase/análise , Isocitrato Liase/metabolismo , Malato Sintase/análise , Malato Sintase/metabolismo , Proteínas Mitocondriais/análise , Modelos Moleculares , Alinhamento de SequênciaRESUMO
Intracytoplasmic sperm injection (ICSI) has been successfully used to produce offspring in several mammalian species including humans. However, ICSI has not been successful in birds because of the size of the egg and difficulty in mimicking the physiological polyspermy that takes place during normal fertilization. Microsurgical injection of 20 or more spermatozoa into an egg is detrimental to its survival. Here, we report that injection of a single spermatozoon with a small volume of sperm extract (SE) or its components led to the development and birth of healthy quail chicks. SE contains three factors - phospholipase Cζ (PLCZ), aconitate hydratase (AH) and citrate synthase (CS) - all of which are essential for full egg activation and subsequent embryonic development. PLCZ induces an immediate, transient Ca(2+) rise required for the resumption of meiosis. AH and CS are required for long-lasting, spiral-like Ca(2+) oscillations within the activated egg, which are essential for cell cycle progression in early embryos. We also found that co-injection of cRNAs encoding PLCZ, AH and CS support the full development of ICSI-generated zygotes without the use of SE. These findings will aid our understanding of the mechanism of avian fertilization and embryo development, as well as assisting in the manipulation of the avian genome and the production of transgenic and cloned birds.
Assuntos
Fertilização/fisiologia , Codorniz/fisiologia , Injeções de Esperma Intracitoplásmicas/veterinária , Espermatozoides/química , Aconitato Hidratase/análise , Animais , Cálcio/metabolismo , Cromatografia Líquida , Citrato (si)-Sintase/análise , Immunoblotting , Masculino , Microscopia de Fluorescência , Óvulo/metabolismo , Fosfoinositídeo Fosfolipase C/análise , Injeções de Esperma Intracitoplásmicas/métodos , Espectrometria de Massas em Tandem , Resultado do TratamentoRESUMO
The molecular mechanisms underlying skeletal muscle aging and associated sarcopenia have been linked to an altered oxidative status of redox-sensitive proteins. Reactive oxygen and reactive nitrogen species (ROS/RNS) generated by contracting skeletal muscle are necessary for optimal protein function, signaling, and adaptation. To investigate the redox proteome of aging gastrocnemius muscles from adult and old male mice, we developed a label-free quantitative proteomic approach that includes a differential cysteine labeling step. The approach allows simultaneous identification of up- and downregulated proteins between samples in addition to the identification and relative quantification of the reversible oxidation state of susceptible redox cysteine residues. Results from muscles of adult and old mice indicate significant changes in the content of chaperone, glucose metabolism, and cytoskeletal regulatory proteins, including Protein DJ-1, cAMP-dependent protein kinase type II, 78 kDa glucose regulated protein, and a reduction in the number of redox-responsive proteins identified in muscle of old mice. Results demonstrate skeletal muscle aging causes a reduction in redox-sensitive proteins involved in the generation of precursor metabolites and energy metabolism, indicating a loss in the flexibility of the redox energy response. Data is available via ProteomeXchange with identifier PXD001054.
Assuntos
Envelhecimento/metabolismo , Cisteína/química , Proteínas Musculares/metabolismo , Músculo Esquelético/fisiologia , Proteômica/métodos , Acetilação , Aconitato Hidratase/análise , Aconitato Hidratase/metabolismo , Sequência de Aminoácidos , Animais , Western Blotting , Chaperona BiP do Retículo Endoplasmático , Frutose-Bifosfato Aldolase/metabolismo , Masculino , Camundongos Endogâmicos C57BL , Dados de Sequência Molecular , Proteínas Musculares/análise , Músculo Esquelético/metabolismo , Oxirredução , Estresse Oxidativo , Espectrometria de Massas em Tandem/métodosRESUMO
In the present study, we tried to answer the following questions: which kind of defense pathways are activated after Aß insult? How defense systems react against noxious effects of Aß and whether they are able to deal against apoptosis or not? So, we traced some molecular pathways including autophagy, mitophagy, and mitochondrial biogenesis before reaching to the endpoint of apoptosis. Besides, we measured the function of mitochondria after injection of Aß (1-42) in CA1 area of hippocampus as a model of Alzheimer's disease (AD). Based on our data, autophagy markers reached to their maximum level and returned to the control level as apoptotic markers started to increase. As a specialized form of autophagy, mitophagy markers followed the trend of autophagy markers. Whereas mitochondrial dynamic processes shifted toward fission, mitochondrial biogenesis was severely affected by Aß and significantly decreased. Alongside suppression of mitochondrial biogenesis, activity of specific enzymes involved in antioxidant defense system, electron transport chain, and tricarboxylic acid cycle (TCA) decreased in response to the Aß. Activity of antioxidant enzymes increased at first and then decreased significantly compared to the control. TCA enzymes aconitase and malate dehydrogenase activities reduced immediately while citrate synthase and fumarase activities did not change. Based on our finding, monitoring of the master molecules of intracellular cascades and determining their trends before the destructive function of Aß could be the target of therapeutic issues for AD.
Assuntos
Peptídeos beta-Amiloides/toxicidade , Apoptose/efeitos dos fármacos , Autofagia/efeitos dos fármacos , Região CA1 Hipocampal/efeitos dos fármacos , Ciclo do Ácido Cítrico/efeitos dos fármacos , Mitofagia/efeitos dos fármacos , Neurônios/efeitos dos fármacos , Fragmentos de Peptídeos/toxicidade , Aconitato Hidratase/análise , Animais , Região CA1 Hipocampal/patologia , Catalase/análise , Citrato (si)-Sintase/análise , Citocromos/análise , Transporte de Elétrons , Indução Enzimática , Fumarato Hidratase/análise , Glutationa/análise , Malato Desidrogenase/análise , Masculino , Microinjeções , Mitocôndrias/efeitos dos fármacos , Mitocôndrias/metabolismo , Proteínas do Tecido Nervoso/análise , Proteínas do Tecido Nervoso/fisiologia , Neurônios/patologia , Proteínas Quinases/análise , Ratos , Ratos Wistar , Espécies Reativas de Oxigênio/metabolismo , Superóxido Dismutase/análise , Fatores de TempoRESUMO
Ferritin is a storage protein that plays a key role in iron metabolism. In this study, we report on the sequence characterization of a ferritin-coding cDNA in Eisenia andrei earthworms isolated by RT-PCR using degenerated primers, and we suggest the presence of a putative IRE in the 5'-UTR of ferritin mRNA. The obtained ferritin sequence was compared with those of other animals showing sequence and structure homology in consensus sites, including the iron-responsive element (IRE) and ferroxidase centers. Despite the sequence homology in the E. andrei mRNA of ferritin with the sequences of other animals in consensus IRE sites, the presented cytosine in the IRE of E. andrei ferritin in the expected position does not form a conventional bulge. The presence of ferritin in the coelomic fluid of E. andrei was proven by iron staining assay. Moreover, aconitase activity in the coelomic fluid was assessed by aconitase assay, suggesting the presence of an iron regulatory protein. Quantitative analysis revealed changes in the gene expression levels of ferritin in coelomocytes in response to bacterial challenge, reaching the maximum level 8h after the stimulation with both Gram-positive and Gram-negative bacteria.
Assuntos
Ferritinas/química , Ferritinas/genética , Oligoquetos/genética , Aconitato Hidratase/análise , Aconitato Hidratase/genética , Aconitato Hidratase/metabolismo , Sequência de Aminoácidos , Animais , Bacillus subtilis/metabolismo , Sequência de Bases , Ceruloplasmina/genética , Clonagem Molecular , Primers do DNA , DNA Complementar/biossíntese , DNA Complementar/genética , DNA Complementar/isolamento & purificação , Escherichia coli K12/metabolismo , Regulação da Expressão Gênica , Dados de Sequência Molecular , Conformação de Ácido Nucleico , Oligoquetos/metabolismo , RNA/genética , RNA/isolamento & purificação , RNA Mensageiro/química , RNA Mensageiro/genética , Reação em Cadeia da Polimerase Via Transcriptase Reversa , Análise de Sequência de DNARESUMO
Aging compromises restoration of the cardiac mechanical function during reperfusion. We hypothesized that this was due to an ampler release of mitochondrial reactive oxygen species (ROS). This study aimed at characterising ex vivo the mitochondrial ROS release during reperfusion in isolated perfused hearts of middle-aged rats. Causes and consequences on myocardial function of the observed changes were then evaluated. The hearts of rats aged 10- or 52-week old were subjected to global ischemia followed by reperfusion. Mechanical function was monitored throughout the entire procedure. Activities of the respiratory chain complexes and the ratio of aconitase to fumarase activities were determined before ischemia and at the end of reperfusion. H(2)O(2) release was also evaluated in isolated mitochondria. During ischemia, middle-aged hearts displayed a delayed contracture, suggesting a maintained ATP production but also an increased metabolic proton production. Restoration of the mechanical function during reperfusion was however reduced in the middle-aged hearts, due to lower recovery of the coronary flow associated with higher mitochondrial oxidative stress indicated by the aconitase to fumarase ratio in the cardiac tissues. Surprisingly, activity of the respiratory chain complex II was better maintained in the hearts of middle-aged animals, probably because of an enhanced preservation of its membrane lipid environment. This can explain the higher mitochondrial oxidative stress observed in these conditions, since cardiac mitochondria produce much more H(2)O(2) when they oxidize FADH(2)-linked substrates than when they use NADH-linked substrates. In conclusion, the lower restoration of the cardiac mechanical activity during reperfusion in the middle-aged hearts was due to an impaired recovery of the coronary flow and an insufficient oxygen supply. The deterioration of the coronary perfusion was explained by an increased mitochondrial ROS release related to the preservation of complex II activity during reperfusion.
Assuntos
Envelhecimento/metabolismo , Vasos Coronários/fisiopatologia , Complexo de Proteínas da Cadeia de Transporte de Elétrons/metabolismo , Contração Miocárdica/fisiologia , Traumatismo por Reperfusão Miocárdica/metabolismo , Espécies Reativas de Oxigênio/metabolismo , Aconitato Hidratase/análise , Animais , Modelos Animais de Doenças , Fumarato Hidratase/análise , Peróxido de Hidrogênio/metabolismo , Técnicas In Vitro , Masculino , Mitocôndrias Cardíacas/metabolismo , Traumatismo por Reperfusão Miocárdica/fisiopatologia , Miocárdio/química , Estresse Oxidativo/fisiologia , Oxigênio/metabolismo , Perfusão , Ratos , Ratos WistarRESUMO
This study examined the time-dependent effects of a cell permeable SOD mimetic, MnTMPyP, on mitochondrial function in renal ischemia-reperfusion injury (IRI). Male SD rats were subject to either sham operation or bilateral renal ischemia for 45 min followed by reperfusion for 1, 4 or 24 h. A sub-set of animals was treated with either saline vehicle or 5 mg/Kg of MnTMPyP (i.p.). EPR measurements showed that at 1-h reperfusion MnTMPyP prevented a decrease in aconitase activity (p < 0.05) and attenuated the increase in the high spin heme at g = 6 and oxidation of 4Fe4S to 3Fe4S signal at g = 2.015 (p < 0.01). MnTMPyP was effective in preventing loss of mitochondrial complexes and prevented the loss of cytochrome c and Smac/Diablo from mitochondria early in reperfusion. Following 24 h of reperfusion MnTMPyP was effective in attenuating caspase-3 and blocking apoptosis (p < 0.05). In conclusion, MnTMPyP has biphasic effects in renal IRI, inhibiting mitochondrial dysfunction at the early phases of reperfusion and prevention of apoptosis following longer durations of reperfusion.
Assuntos
Antioxidantes/uso terapêutico , Rim/irrigação sanguínea , Metaloporfirinas/uso terapêutico , Mitocôndrias/efeitos dos fármacos , Traumatismo por Reperfusão/tratamento farmacológico , Aconitato Hidratase/análise , Animais , Antioxidantes/farmacologia , Apoptose/efeitos dos fármacos , Proteínas Reguladoras de Apoptose , Proteínas de Transporte/análise , Caspase 3/análise , Citocromos c/análise , Avaliação Pré-Clínica de Medicamentos , Espectroscopia de Ressonância de Spin Eletrônica , Heme/análise , Marcação In Situ das Extremidades Cortadas , Masculino , Metaloporfirinas/farmacologia , Mitocôndrias/fisiologia , Proteínas Mitocondriais/análise , Oxirredução , Estresse Oxidativo/efeitos dos fármacos , Ratos , Ratos Sprague-Dawley , Espécies Reativas de Oxigênio/metabolismo , Traumatismo por Reperfusão/metabolismo , Superóxido Dismutase/análise , Fatores de TempoRESUMO
PURPOSE: In patients with mesial temporal lobe epilepsy (MTLE) it remains an unresolved issue whether the interictal decrease in N-acetyl aspartate (NAA) detected by proton magnetic resonance spectroscopy ((1)H-MRS) reflects the epilepsy-associated loss of hippocampal pyramidal neurons or metabolic dysfunction. METHODS: To address this problem, we applied high-resolution (1)H-MRS at 14.1 Tesla to measure metabolite concentrations in ex vivo tissue slices from three hippocampal subfields (CA1, CA3, dentate gyrus) as well as from the parahippocampal region of 12 patients with MTLE. RESULTS: In contrast to four patients with lesion-caused MTLE, we found a large variance of NAA concentrations in the individual hippocampal regions of patients with Ammon's horn sclerosis (AHS). Specifically, in subfield CA3 of AHS patients despite of a moderate preservation of neuronal cell densities the concentration of NAA was significantly lowered, while the concentrations of lactate, glucose, and succinate were elevated. We suggest that these subfield-specific alterations of metabolite concentrations in AHS are very likely caused by impairment of mitochondrial function and not related to neuronal cell loss. CONCLUSIONS: A subfield-specific impairment of energy metabolism is the probable cause for lowered NAA concentrations in sclerotic hippocampi of MTLE patients.
Assuntos
Ácido Aspártico/análogos & derivados , Epilepsia do Lobo Temporal/metabolismo , Hipocampo/química , Aconitato Hidratase/análise , Adulto , Idoso , Ácido Aspártico/análise , Ácido Aspártico/metabolismo , Contagem de Células , Eletroencefalografia/estatística & dados numéricos , Epilepsia do Lobo Temporal/patologia , Epilepsia do Lobo Temporal/cirurgia , Feminino , Glucose/análise , Glucose/metabolismo , Hipocampo/metabolismo , Hipocampo/patologia , Humanos , Lactatos/análise , Lactatos/metabolismo , Imageamento por Ressonância Magnética/estatística & dados numéricos , Espectroscopia de Ressonância Magnética/estatística & dados numéricos , Masculino , Pessoa de Meia-Idade , Mitocôndrias/enzimologia , Doenças Mitocondriais/metabolismo , Doenças Mitocondriais/patologia , Giro Para-Hipocampal/química , Giro Para-Hipocampal/metabolismo , Giro Para-Hipocampal/patologia , Cuidados Pré-Operatórios , Células Piramidais/química , Células Piramidais/metabolismo , Células Piramidais/patologia , Esclerose , Succinatos/análise , Succinatos/metabolismoRESUMO
Zinc is an essential metal that, when in excess, can be deleterious to the cell. Therefore, homeostatic mechanisms for this cation must be finely tuned. To better understand the response of yeast in front of an excess of zinc, we screened a systematic deletion mutant library for altered growth in the presence of 6 mM zinc. Eighty-nine mutants exhibited increased zinc sensitivity, including many genes involved in vacuolar assembling and biogenesis. Interestingly, a mutant lacking the Aft1 transcription factor, required for the transcriptional response to iron starvation, was found to be highly sensitive to zinc. Genome-wide transcriptional profiling revealed that exposure to 5 mM ZnCl(2) results in rapid increase in the expression of numerous chaperones required for proper protein folding or targeting to vacuole and mitochondria, as well as genes involved in stress response (mainly oxidative), sulphur metabolism and some components of the iron regulon. The effect of the lack of Aft1 both in the absence and in the presence of zinc overload was also investigated. Exposure to high zinc generated reactive oxygen species and markedly decreased glutathione content. Interestingly, zinc excess results in decreased intracellular iron content and aconitase and cytochrome c activities in stationary-phase cultures. These findings suggest that high zinc levels may alter the assembly and/or function of iron-sulphur-containing proteins, as well as the biosynthesis of haem groups, thus establishing a link between zinc, iron and sulphur metabolism.
Assuntos
Ferro/metabolismo , Proteínas de Saccharomyces cerevisiae/metabolismo , Saccharomyces cerevisiae/efeitos dos fármacos , Zinco/toxicidade , Aconitato Hidratase/análise , Aconitato Hidratase/metabolismo , Cloretos/farmacologia , Citocromos c/metabolismo , Perfilação da Expressão Gênica , Genes Fúngicos , Genoma Fúngico , Glutationa/metabolismo , Homeostase/genética , Proteínas Ferro-Enxofre/genética , Proteínas Ferro-Enxofre/metabolismo , Estresse Oxidativo , Espécies Reativas de Oxigênio/metabolismo , Saccharomyces cerevisiae/genética , Saccharomyces cerevisiae/crescimento & desenvolvimento , Proteínas de Saccharomyces cerevisiae/genética , Fatores de Transcrição/genética , Transcrição Gênica , Zinco/metabolismo , Compostos de Zinco/farmacologiaRESUMO
A system for the controlled expression of genes in Azotobacter vinelandii by using genomic fusions to the sucrose catabolic regulon was developed. This system was used for the functional analysis of the A. vinelandii isc genes, whose products are involved in the maturation of [Fe-S] proteins. For this analysis, the scrX gene, contained within the sucrose catabolic regulon, was replaced by the contiguous A. vinelandii iscS, iscU, iscA, hscB, hscA, fdx, and iscX genes, resulting in duplicate genomic copies of these genes: one whose expression is directed by the normal isc regulatory elements (Pisc) and the other whose expression is directed by the scrX promoter (PscrX). Functional analysis of [Fe-S] protein maturation components was achieved by placing a mutation within a particular Pisc-controlled gene with subsequent repression of the corresponding PscrX-controlled component by growth on glucose as the carbon source. This experimental strategy was used to show that IscS, IscU, HscBA, and Fdx are essential in A. vinelandii and that their depletion results in a deficiency in the maturation of aconitase, an enzyme that requires a [4Fe-4S] cluster for its catalytic activity. Depletion of IscA results in a null growth phenotype only when cells are cultured under conditions of elevated oxygen, marking the first null phenotype associated with the loss of a bacterial IscA-type protein. Furthermore, the null growth phenotype of cells depleted of HscBA could be partially reversed by culturing cells under conditions of low oxygen. Conserved amino acid residues within IscS, IscU, and IscA that are essential for their respective functions and/or whose replacement results in a partial or complete dominant-negative growth phenotype were also identified using this system.
Assuntos
Azotobacter vinelandii/metabolismo , Proteínas de Bactérias/fisiologia , Genes Bacterianos , Proteínas Ferro-Enxofre/biossíntese , Aconitato Hidratase/análise , Aerobiose , Fusão Gênica Artificial , Azotobacter vinelandii/genética , Azotobacter vinelandii/crescimento & desenvolvimento , Proteínas de Bactérias/genética , Sequência Conservada , Deleção de Genes , Duplicação Gênica , Regulação Bacteriana da Expressão Gênica , Genes Essenciais , Glucose/metabolismo , Família Multigênica , Mutação , Oxigênio/metabolismoRESUMO
BACKGROUND: Mitochondrial (m) aconitase plays an important role in the unique pathway of citrate accumulation in prostate epithelial cells through its limited activity. In the current study, we characterized the human m-aconitase gene promoter. METHODS: A 1,411-bp 5'-flanking fragment of the human m-aconitase gene was cloned, followed by 5' serial deletion analysis of promoter activity. Transcriptional start sties and transcription factors bound to the promoter were identified by 5' RACE, DNA pull-down assay and transcription factor array analysis. RESULTS: Two transcriptional start sites were identified. The promoter fragment pulled down 15 transcription factors, some without consensus sequences in the promoter. Deletion of one Sp1 site eliminated all promoter activity. CONCLUSIONS: The m-aconitase promoter is contained in a 153-bp 5' fragment lacking a TATA or CAAT sequence. Sp1 binding to a specific Sp1 site is required for promoter activity while other transcription factors are recruited through protein-protein interactions.
Assuntos
Aconitato Hidratase/genética , Mitocôndrias/enzimologia , Regiões Promotoras Genéticas/genética , Fatores de Transcrição/genética , Aconitato Hidratase/análise , Aconitato Hidratase/fisiologia , Sequência de Bases , Fator de Ligação a CCAAT/genética , Fator de Ligação a CCAAT/fisiologia , Linhagem Celular Tumoral , DNA/análise , DNA/genética , Epitélio/enzimologia , Regulação da Expressão Gênica , Humanos , Masculino , Dados de Sequência Molecular , Análise de Sequência com Séries de Oligonucleotídeos , Regiões Promotoras Genéticas/fisiologia , Próstata/enzimologia , Ligação Proteica/genética , Ligação Proteica/fisiologia , Fator de Transcrição Sp1/análise , Fator de Transcrição Sp1/genética , Fator de Transcrição Sp1/fisiologia , Proteína de Ligação a TATA-Box/genética , Proteína de Ligação a TATA-Box/fisiologia , Fatores de Transcrição/análise , Fatores de Transcrição/fisiologia , Transcrição Gênica/genética , Transcrição Gênica/fisiologiaRESUMO
BACKGROUND: In prostate cancer, normal citrate-producing glandular secretory epithelial cells undergo a metabolic transformation to malignant citrate-oxidizing cells. m-Aconitase is the critical step involved in this altered citrate metabolism that is essential to prostate malignancy. The limiting m-aconitase activity in prostate epithelial cells could be the result of a decreased level of m-aconitase enzyme and/or the inhibition of existing m-aconitase. Earlier studies identified zinc as an inhibitor of m-aconitase activity in prostate cells; and that the depletion of zinc in malignant cells is an important factor in this metabolic transformation. However, a possibility remains that an altered expression and level of m-aconitase enzyme might also be involved in this metabolic transformation. To address this issue, the in situ level of m-aconitase enzyme was determined by immunohistochemical analysis of prostate cancer tissue sections and malignant prostate cell lines. RESULTS: The immunocytochemical procedure successfully identified the presence of m-aconitase localized in the mitochondrial compartment in PC-3, LNCaP, and DU-145 malignant prostate cell lines. The examination of prostate tissue sections from prostate cancer subjects demonstrated that m-aconitase enzyme is present in the glandular epithelium of normal glands, hyperplastic glands, adenocrcinomatous glands, and prostatic intraepithelial neoplastic foci. Quantitative analysis of the relative level of m-aconitase in the glandular epithelium of citrate-producing adenomatous glands versus the citrate-oxidizing adenocarcinomatous glands revealed no significant difference in m-aconitase enzyme levels. This is in contrast to the down-regulation of ZIP1 zinc transporter in the malignant glands versus hyperplastic glands that exists in the same tissue samples. CONCLUSION: The results demonstrate the existence of m-aconitase enzyme in the citrate-producing glandular epithelial cells; so that deficient m-aconitase enzyme is not associated with the limiting m-aconitase activity that prevents citrate oxidation in these cells. The level of m-aconitase is maintained in the malignant cells; so that an altered enzyme level is not associated with the increased m-aconitase activity. Consequently, the elevated zinc level that inhibits m-aconitase enzyme is responsible for the impaired citrate oxidation in normal and hyperplastic prostate glandular epithelial cells. Moreover, the down-regulation of ZIP1 zinc transporter and corresponding depletion of zinc results in the increase in the activity of the existing m-aconitase activity in the malignant prostate cells. The studies now define the mechanism for the metabolic transformation that characterizes the essential transition of normal citrate-producing epithelial cells to malignant citrate-oxidizing cells.
Assuntos
Aconitato Hidratase/metabolismo , Ácido Cítrico/metabolismo , Mitocôndrias/enzimologia , Próstata/enzimologia , Neoplasias da Próstata/enzimologia , Aconitato Hidratase/análise , Linhagem Celular Tumoral , Humanos , MasculinoRESUMO
The NADP(H)-dependent enzymes glucose-6-phosphate dehydrogenase (G6PDH) and ferredoxin(flavodoxin)-NADP(H) reductase (FPR), encoded by the zwf and fpr genes, respectively, are committed members of the soxRS regulatory system involved in superoxide resistance in Escherichia coli. Exposure of E. coli cells to the superoxide propagator methyl viologen (MV) led to rapid accumulation of G6PDH, while FPR was induced after a lag period of several minutes. Bacteria expressing G6PDH from a multicopy plasmid accumulated higher NADPH levels and displayed a protracted soxRS response, whereas FPR build-up had the opposite effects. Inactivation of either of the two genes resulted in enhanced sensitivity to MV killing, while further increases in the cellular content of FPR led to higher survival rates under oxidative conditions. In contrast, G6PDH accumulation over wild-type levels of expression failed to increase MV tolerance. G6PDH and FPR could act concertedly to deliver reducing equivalents from carbohydrates, via NADP(+), to the FPR acceptors ferredoxin and/or flavodoxin. To evaluate whether this electron-transport system could mediate reductive repair reactions, the pathway was reconstituted in vitro from purified components; the reconstituted system was found to be functional in reactivation of oxidatively damaged iron-sulfur clusters of hydro-lyases such as aconitase and 6-phosphogluconate dehydratase. Recovery of these activities after oxidative challenge was faster and more extensive in transformed bacteria overexpressing FPR than in wild-type cells, indicating that the reductase could sustain hydro-lyase repair in vivo. However, FPR-deficient mutants were still able to fix iron-sulfur clusters at significant rates, suggesting that back-up routes for ferredoxin and/or flavodoxin reduction might be called into action to rescue inactivated enzymes when FPR is absent.
Assuntos
Proteínas de Bactérias/fisiologia , Proteínas de Escherichia coli/fisiologia , Escherichia coli/fisiologia , Ferredoxina-NADP Redutase/fisiologia , Glucosefosfato Desidrogenase/fisiologia , Estresse Oxidativo , Transativadores/fisiologia , Fatores de Transcrição/fisiologia , Aconitato Hidratase/análise , Adaptação Fisiológica , Fusão Gênica Artificial , Transporte de Elétrons , Escherichia coli/enzimologia , Ferredoxinas/metabolismo , Flavodoxina/metabolismo , Deleção de Genes , Regulação Bacteriana da Expressão Gênica , Genes Reporter , Hidroliases/análise , Mutagênese Insercional , Paraquat , Regulon , Superóxidos/metabolismo , Superóxidos/toxicidade , beta-Galactosidase/análise , beta-Galactosidase/genéticaRESUMO
Staphylococcal polysaccharide intercellular adhesin (PIA) is important for the development of a mature biofilm. PIA production is increased during growth in a nutrient-replete or iron-limited medium and under conditions of low oxygen availability. Additionally, stress-inducing stimuli such as heat, ethanol, and high concentrations of salt increase the production of PIA. These same environmental conditions are known to repress tricarboxylic acid (TCA) cycle activity, leading us to hypothesize that altering TCA cycle activity would affect PIA production. Culturing Staphylococcus epidermidis with a low concentration of the TCA cycle inhibitor fluorocitrate dramatically increased PIA production without impairing glucose catabolism, the growth rate, or the growth yields. These data lead us to speculate that one mechanism by which staphylococci perceive external environmental change is through alterations in TCA cycle activity leading to changes in the intracellular levels of biosynthetic intermediates, ATP, or the redox status of the cell. These changes in the metabolic status of the bacteria result in the attenuation or augmentation of PIA production.
Assuntos
Ciclo do Ácido Cítrico , Polissacarídeos Bacterianos/biossíntese , Staphylococcus epidermidis/metabolismo , Ácido Acético/metabolismo , Aconitato Hidratase/análise , Adaptação Fisiológica , Biomassa , Citratos/farmacologia , Ciclo do Ácido Cítrico/efeitos dos fármacos , Regulação Bacteriana da Expressão Gênica , Glucose/metabolismo , Isocitrato Desidrogenase/análise , NAD/análise , Oxirredução , Staphylococcus epidermidis/efeitos dos fármacos , Staphylococcus epidermidis/crescimento & desenvolvimentoRESUMO
Three pairs of parental (rho+) and established mitochondrial DNA depleted (rho0) cells, derived from bone, lung and muscle were used to verify the influence of the nuclear background and the lack of efficient mitochondrial respiratory chain on antioxidant defences and homeostasis of intracellular reactive oxygen species (ROS). Mitochondrial DNA depletion significantly lowered glutathione reductase activity, glutathione (GSH) content, and consistently altered the GSH2 : oxidized glutathione ratio in all of the rho0 cell lines, albeit to differing extents, indicating the most oxidized redox state in bone rho0 cells. Activity, as well as gene expression and protein content, of superoxide dismutase showed a decrease in bone and muscle rho0 cell lines but not in lung rho0 cells. GSH peroxidase activity was four times higher in all three rho0 cell lines in comparison to the parental rho+, suggesting that this may be a necessary adaptation for survival without a functional respiratory chain. Taken together, these data suggest that the lack of respiratory chain prompts the cells to reduce their need for antioxidant defences in a tissue-specific manner, exposing them to a major risk of oxidative injury. In fact bone-derived rho0 cells displayed the highest steady-state level of intracellular ROS (measured directly by 2',7'-dichlorofluorescin, or indirectly by aconitase activity) compared to all the other rho+ and rho0 cells, both in the presence or absence of glucose. Analysis of mitochondrial and cytosolic/iron regulatory protein-1 aconitase indicated that most ROS of bone rho0 cells originate from sources other than mitochondria.
Assuntos
Antioxidantes/metabolismo , DNA Mitocondrial/metabolismo , Espécies Reativas de Oxigênio/metabolismo , Aconitato Hidratase/análise , Aconitato Hidratase/metabolismo , Western Blotting , Carcinoma/metabolismo , Catalase/análise , Catalase/metabolismo , Linhagem Celular Tumoral , Glutationa/análise , Glutationa/metabolismo , Glutationa Peroxidase/análise , Glutationa Peroxidase/metabolismo , Glutationa Redutase/análise , Glutationa Redutase/metabolismo , Glutationa Transferase/análise , Glutationa Transferase/metabolismo , Homeostase , Humanos , Neoplasias Pulmonares/metabolismo , Osteossarcoma/metabolismo , Rabdomiossarcoma/metabolismo , Frações Subcelulares/enzimologia , Superóxido Dismutase/análise , Superóxido Dismutase/metabolismoAssuntos
Antibióticos Antineoplásicos/toxicidade , Apoptose/efeitos dos fármacos , Doxorrubicina/toxicidade , Ferro/metabolismo , Oxidantes/farmacologia , Transdução de Sinais , Aconitato Hidratase/análise , Animais , Antibióticos Antineoplásicos/metabolismo , Antioxidantes/farmacologia , Caspases/metabolismo , Células Cultivadas , Quelantes/farmacologia , Citocromos c/metabolismo , Doxorrubicina/metabolismo , Endotélio/efeitos dos fármacos , Endotélio/metabolismo , Cardiopatias/induzido quimicamente , Cardiopatias/tratamento farmacológico , Cardiopatias/fisiopatologia , Humanos , Quelantes de Ferro/uso terapêutico , Proteína 1 Reguladora do Ferro/metabolismo , Proteínas Reguladoras de Ferro/metabolismo , Mitocôndrias/enzimologia , Mitocôndrias/metabolismo , Células Musculares/efeitos dos fármacos , Células Musculares/metabolismo , Oxidantes/análise , Oxirredução , Estresse Oxidativo , RNA Mensageiro/análise , RNA Mensageiro/metabolismo , Razoxano/farmacologia , Espécies Reativas de Oxigênio/metabolismo , Receptores da Transferrina/análise , Receptores da Transferrina/efeitos dos fármacos , Receptores da Transferrina/metabolismo , Elementos de Resposta , Superóxidos/análise , Regulação para CimaRESUMO
Iron regulatory protein 1 (IRP1) post-transcriptionally regulates the expression of proteins involved in the iron metabolism of mammals. IRP1 is a bifunctional cytosolic protein which can exhibit aconitase activity or bind to iron responsive element (IREs) in the untranslated regions of specific mRNAs. The modulation of IRP1 activities and its consequence for intracellular iron homeostasis is best characterized in rodents and humans. Little is known about IRP1 in farm animals. In this study, we analyzed the two activities of IRP1 in the livers of four farm animal species (cattle, goat, pig and rabbit) and their relationship to hepatic iron content. We found an inverse correlation between spontaneous IRP1 IRE binding activity and non-haem iron content in the liver. Using the electrophoretic mobility shift assay, we showed differential mobility of IRE/IRP1 complexes formed with hepatic cytosolic extracts from various farm animal species. We discuss this observation in relation to a comparative analysis of mammalian IRP1 amino acid sequences.
Assuntos
Animais Domésticos/metabolismo , Proteína 1 Reguladora do Ferro/metabolismo , Ferro/metabolismo , Fígado/metabolismo , Aconitato Hidratase/análise , Aconitato Hidratase/metabolismo , Sequência de Aminoácidos , Animais , Bovinos , Ensaio de Desvio de Mobilidade Eletroforética , Ferro/análise , Fígado/química , Fígado/enzimologia , Camundongos , Dados de Sequência Molecular , Coelhos , Elementos de Resposta/genética , Homologia de Sequência de Aminoácidos , Regiões não Traduzidas/metabolismoRESUMO
We have previously reported the usefulness of two-dimensional gel electrophoresis followed by Western blotting with sera from patients with systemic candidiasis in the detection of the major Candida albicans antigens (Pitarch et al., Electrophoresis 1999, 20, 1001-1010). The identification of these antigens would be useful for the characterization of good markers for the disease, and for the development of efficient diagnostic strategies. In this work we have used nanoelectrospray tandem mass spectrometry to obtain amino acid sequence information from the immunogenic proteins previously detected. We report here the cross-species identification of these antigens by matching of tandem mass spectrometry data to Saccharomyces cerevisiae proteins. Using this approach, we unambiguously identified the four C. albicans immunogenic proteins analyzed, namely aconitase, pyruvate kinase, phosphoglycerate mutase and methionine synthase. Furthermore, we report for the first time that aconitase, methionine synthase and phosphoglycerate mutase have antigenic properties in C. albicans.
Assuntos
Antígenos de Fungos/imunologia , Candida albicans/imunologia , Eletroforese em Gel Bidimensional/métodos , Proteínas Fúngicas/imunologia , Saccharomyces cerevisiae/imunologia , Espectrometria de Massas por Ionização por Electrospray/métodos , Espectrometria de Massas por Ionização e Dessorção a Laser Assistida por Matriz/métodos , 5-Metiltetra-Hidrofolato-Homocisteína S-Metiltransferase/análise , 5-Metiltetra-Hidrofolato-Homocisteína S-Metiltransferase/imunologia , Aconitato Hidratase/análise , Aconitato Hidratase/imunologia , Sequência de Aminoácidos , Antígenos de Fungos/análise , Candida albicans/química , Bases de Dados Factuais , Proteínas Fúngicas/análise , Dados de Sequência Molecular , Fosfoglicerato Mutase/análise , Fosfoglicerato Mutase/imunologia , Piruvato Quinase/análise , Piruvato Quinase/imunologia , Saccharomyces cerevisiae/química , Alinhamento de Sequência , Homologia de Sequência de Aminoácidos , Especificidade da EspécieRESUMO
The metabolic consequences of two insertions, iscR1::MudJ and iscA2::MudJ, in the isc gene cluster of Salmonella enterica serovar Typhimurium were studied. Each of these insertions had polar effects and caused a nutritional requirement for the thiazole moiety of thiamine. Data showed that IscS was required for the synthesis of nicotinic acid and the thiazole moiety of thiamine and that one or more additional isc gene products were required for a distinct step in the thiazole biosynthetic pathway. Strains with isc lesions had reduced succinate dehydrogenase and aconitase activities. Furthermore, isc mutants accumulated increased levels of pyruvate in the growth medium in response to exogenously added iron (FeCl(3)), and this response required a functional ferric uptake regulator, Fur.