RESUMO
Research on modulation of iodine uptake by thyroid cells could help improve radioiodine treatment of dogs with thyroid tumors. The aim of this study was to characterize the immunohistochemical expression of thyroid transcription factor-1 (TTF-1), thyroglobulin, thyrotropin receptor (TSHR), sodium iodide symporter (NIS), pendrin, thyroid peroxidase (TPO), vimentin, and Ki-67 in follicular cell thyroid carcinomas (FTCs) and medullary thyroid carcinomas (MTCs), and to compare protein expression between FTC causing hyperthyroidism and FTC of euthyroid dogs. Immunohistochemistry was performed in 25 FTCs (9 follicular, 8 follicular-compact, and 8 compact) and 8 MTCs. FTCs and MTCs were positive for TTF-1, and expression was higher in FTCs of euthyroid dogs compared with FTCs of hyperthyroid dogs (P= .041). Immunolabeling for thyroglobulin was higher in follicular and follicular-compact FTCs compared with compact FTCs (P = .001), while vimentin expression was higher in follicular-compact FTCs compared with follicular FTCs (P = .011). The expression of TSHR, NIS, pendrin, and TPO was not significantly different among the different subtypes of FTCs or between FTCs causing hyperthyroidism and FTCs in euthyroid dogs. TSHR, NIS, pendrin, and TPO were also expressed in MTCs. Ki-67 labeling index was comparable between FTCs and MTCs, and between FTCs causing hyperthyroidism and FTCs in euthyroid dogs. Proteins of iodine transport were also expressed in canine MTCs, which could have implications for diagnosis and treatment. The different expression of thyroglobulin and vimentin between FTC histological subtypes could reflect variations in tumor differentiation.
Assuntos
Adenocarcinoma Folicular , Carcinoma Neuroendócrino , Doenças do Cão , Imuno-Histoquímica , Neoplasias da Glândula Tireoide , Cães , Animais , Neoplasias da Glândula Tireoide/veterinária , Neoplasias da Glândula Tireoide/patologia , Neoplasias da Glândula Tireoide/metabolismo , Doenças do Cão/metabolismo , Doenças do Cão/patologia , Imuno-Histoquímica/veterinária , Carcinoma Neuroendócrino/veterinária , Carcinoma Neuroendócrino/patologia , Carcinoma Neuroendócrino/metabolismo , Adenocarcinoma Folicular/veterinária , Adenocarcinoma Folicular/patologia , Adenocarcinoma Folicular/metabolismo , Tireoglobulina/metabolismo , Masculino , Simportadores/metabolismo , Feminino , Receptores da Tireotropina/metabolismo , Iodeto Peroxidase/metabolismo , Vimentina/metabolismo , Fator Nuclear 1 de Tireoide/metabolismo , Hipertireoidismo/veterinária , Hipertireoidismo/metabolismo , Hipertireoidismo/patologia , Antígeno Ki-67/metabolismoRESUMO
Canine thyroid carcinomas are relatively common malignant endocrine neoplasms in dogs derived from either thyroid follicular cells (forming follicular thyroid carcinomas) or medullary cells (parafollicular, C-cells; forming medullary thyroid carcinomas). Older and recent clinical studies often fail to discriminate between compact cellular (solid) follicular thyroid carcinomas and medullary thyroid carcinomas, which may skew conclusions. The compact subtype of follicular thyroid carcinomas appears to be the least differentiated subtype of follicular thyroid carcinomas and needs to be differentiated from medullary thyroid carcinomas. This review includes information on the signalment, presentation, etiopathogenesis, classification, histologic and immunohistochemical diagnosis, clinical management, and biochemical and genetic derangements of canine follicular and medullary carcinomas, and their correlates with human medicine.
Assuntos
Adenocarcinoma Folicular , Carcinoma Medular , Carcinoma Neuroendócrino , Doenças do Cão , Neoplasias da Glândula Tireoide , Humanos , Cães , Animais , Neoplasias da Glândula Tireoide/diagnóstico , Neoplasias da Glândula Tireoide/veterinária , Adenocarcinoma Folicular/diagnóstico , Adenocarcinoma Folicular/veterinária , Carcinoma Neuroendócrino/diagnóstico , Carcinoma Neuroendócrino/veterinária , Carcinoma Medular/patologia , Carcinoma Medular/veterinária , Doenças do Cão/diagnósticoRESUMO
A 10-year-old female mixed breed dog was presented for thyroid scintigraphy due to a cervical mass. Apart from 99m-Technetium-pertechnetate (Tc-pertechnetate) scintigraphy, a second scintigraphy using 99m-Technetium sestamibi (Tc-MIBI) was performed because of additional hypothyroidism suspective for a "cold" nodule and as screening for metastases.Twenty minutes following intravenous injection of 38 MBq Tc-pertechnetate, a "hot" cervical as well as a "hot" intrathoracal nodule were seen with an uptake of 8.40 and 0.25 %, respectively. The second scintigraphy was performed 20 minutes after intravenous injection of 364 MBq Tc-MIBI and 70 minutes following the first. After subtraction of pertechnetate activity and decay correction, both nodules showed an uptake of 0.99 and 0.03 %. Additionally, both thyroid lobes were visible in the thyroid loge with a weak MIBI-uptake. For both lesions, the ratio Tc-uptake/Tc-MIBI-uptake was 8.48 and 8.33, respectively.Following the extirpation of the cervical mass, histopathology revealed atrophied healthy thyroid tissue almost completely displaced by a well-differentiated follicular thyroid carcinoma.This case report describes performance, utility and calculative correction of consecutive pertechnetate- and MIBI-scan, that enable a visualization of hypofunctional thyroid tissue.Therefore and because of their similar MIBI metabolic activity, both nodules were considered to be dystopic tissue/metastases so that this dog had to be classified as prognostically less favorable WHO stabe IV. Different from human patients, both scintigraphies should be performed shortly after another in dogs in order to avoid the necessity of a second anesthetic procedure. A reliable qualitative/visual evaluation of the MIBI-scan is therefore not possible, so that a quantitative assessment using the uptake after calculative correction of the pertechnetate activity is recommended.
Assuntos
Adenocarcinoma Folicular , Doenças do Cão , Neoplasias da Glândula Tireoide , Nódulo da Glândula Tireoide , Humanos , Feminino , Cães , Animais , Pertecnetato Tc 99m de Sódio , Nódulo da Glândula Tireoide/diagnóstico por imagem , Nódulo da Glândula Tireoide/patologia , Nódulo da Glândula Tireoide/veterinária , Tecnécio , Tecnécio Tc 99m Sestamibi , Neoplasias da Glândula Tireoide/diagnóstico por imagem , Neoplasias da Glândula Tireoide/veterinária , Cintilografia , Adenocarcinoma Folicular/veterinária , Doenças do Cão/diagnóstico por imagemRESUMO
A 12-y-old, castrated male Weimaraner dog was presented for a wellness examination. A 7-cm, firm mass was palpated on the left, ventral, mid-lateral neck. The neck mass was removed surgically and submitted for histopathology. A thyroid carcinoma was diagnosed based on microscopic examination. Immunohistochemistry for chromogranin-A, calcitonin, and thyroglobulin identified dual immunoreactivity of the latter two, and a final diagnosis was of a well-differentiated, compact, mixed medullary and follicular cell thyroid carcinoma. These neoplasms are rare in humans and have not been reported in dogs, to our knowledge.
Assuntos
Adenocarcinoma Folicular , Carcinoma Medular , Doenças do Cão , Neoplasias da Glândula Tireoide , Humanos , Cães , Masculino , Animais , Tireoglobulina , Calcitonina , Carcinoma Medular/patologia , Carcinoma Medular/ultraestrutura , Carcinoma Medular/veterinária , Cromograninas , Adenocarcinoma Folicular/diagnóstico , Adenocarcinoma Folicular/cirurgia , Adenocarcinoma Folicular/veterinária , Neoplasias da Glândula Tireoide/cirurgia , Neoplasias da Glândula Tireoide/veterinária , Neoplasias da Glândula Tireoide/patologia , Doenças do Cão/diagnóstico , Doenças do Cão/cirurgia , Doenças do Cão/patologiaRESUMO
Thyroid carcinomas (TCs) originating from follicular cells of the thyroid gland occur in both humans and dogs, and they have highly similar histomorphologic patterns. In dogs, TCs have not been extensively investigated, especially concerning the familial origin of TCs. Here, we report familial thyroid follicular cell carcinomas (FCCs) confirmed by histology in 54 Dutch origin German longhaired pointers. From the pedigree, 45 of 54 histopathologically confirmed cases are closely related to a pair of first-half cousins in the past, indicating a familial disease. In addition, genetics contributed more to the thyroid FCC than other factors by an estimated heritability of 0.62 based on pedigree. The age of diagnosis ranged between 4.5 and 13.5 years, and 76% of cases were diagnosed before 10 years of age, implying an early onset of disease. We observed a significant higher pedigree-based inbreeding coefficient in the affected dogs (mean F, 0.23) compared to unaffected dogs (mean F, 0.14), suggesting the contribution of inbreeding to tumour development. The unique occurrence of familial thyroid FCC in this dog population and the large number of affected dogs make this population an important model to identify the genetic basis of familial thyroid FCC in this breed and may contribute to the research into pathogenesis, prevention and treatment in humans.
Assuntos
Adenocarcinoma Folicular , Doenças do Cão , Neoplasias da Glândula Tireoide , Adenocarcinoma Folicular/genética , Adenocarcinoma Folicular/patologia , Adenocarcinoma Folicular/veterinária , Animais , Doenças do Cão/epidemiologia , Doenças do Cão/genética , Doenças do Cão/patologia , Cães , Endogamia , Linhagem , Neoplasias da Glândula Tireoide/epidemiologia , Neoplasias da Glândula Tireoide/genética , Neoplasias da Glândula Tireoide/veterináriaRESUMO
Familial thyroid cancer originating from follicular cells accounts for 5-15% of all the thyroid carcinoma cases in humans. Previously, we described thyroid follicular cell carcinomas in a large number of the Dutch German longhaired pointers (GLPs) with a likely autosomal recessive inheritance pattern. Here, we investigated the genetic causes of the disease using a combined approach of genome-wide association study and runs of homozygosity (ROH) analysis based on 170k SNP array genotype data and whole-genome sequences. A region 0-5 Mb on chromosome 17 was identified to be associated with the disease. Whole-genome sequencing revealed many mutations fitting the recessive inheritance pattern in this region including two deleterious mutations in the TPO gene, chr17:800788G>A (686F>V) and chr17:805276C>T (845T>M). These two SNP were subsequently genotyped in 186 GLPs (59 affected and 127 unaffected) and confirmed to be highly associated with the disease. The recessive genotypes had higher relative risks of 16.94 and 16.64 compared to homozygous genotypes for the reference alleles, respectively. This study provides novel insight into the genetic causes leading to the familial thyroid follicular cell carcinoma, and we were able to develop a genetic test to screen susceptible dogs.
Assuntos
Adenocarcinoma Folicular/genética , Doenças do Cão/genética , Iodeto Peroxidase/genética , Mutação , Adenocarcinoma Folicular/veterinária , Animais , Cães , Feminino , Masculino , Polimorfismo de Nucleotídeo ÚnicoRESUMO
Thyroid carcinomas are a common form of endocrine neoplasia in dogs. In the present study, we combined histopathology with immunohistochemistry (IHC) to search for the presence of oestrogen receptor alpha (ORα), Cox-2 and Ki67 in canine thyroid carcinomas. Forty-eight thyroid carcinomas were diagnosed throughout the study period. Thyroglobulin and calcitonin IHC distinguished between thyroid tumours with a follicular and medullary (C-cell) origin, respectively. IHC-based diagnosis showed that 42 (87.50%) of the cases were follicular cell carcinoma. In these cases, the follicular-compact pattern was the most frequent (n = 20/42; 47.62%) and six cases (12.5%) were medullary cell (C-cell) carcinomas. Both medullary (C-cell) and follicular carcinomas expressed Ki67 and Cox-2. No differences were observed between medullary and follicular carcinomas with respect to expression of Ki67 (P = 0.34) and Cox-2 (P = 0.9523) markers. A total of 4.17% (n = 2/48) of thyroid carcinomas showed positive nuclear labelling for ORα, suggesting that oestrogen does not directly participate in the pathogenesis of canine thyroid neoplasia.
Assuntos
Doenças do Cão , Neoplasias da Glândula Tireoide/veterinária , Adenocarcinoma Folicular/patologia , Adenocarcinoma Folicular/veterinária , Animais , Biomarcadores Tumorais/metabolismo , Calcitonina/metabolismo , Carcinoma Neuroendócrino/patologia , Carcinoma Neuroendócrino/veterinária , Diagnóstico Diferencial , Cães , Neoplasias das Glândulas Endócrinas/veterinária , Imuno-Histoquímica/veterinária , Tireoglobulina/metabolismo , Neoplasias da Glândula Tireoide/patologiaRESUMO
An 18-y-old female llama (Lama glama) in Saskatoon, Saskatchewan was examined during a routine herd check, and a mass was detected in the ventral cervical area just below the angle of the jaw. No clinical signs were evident except for the mass and chronic loss of body condition. Postmortem examination revealed bilateral enlargement of the thyroid gland with multiple cysts. Histopathology of the thyroid gland revealed follicular compact-cellular carcinoma lesions, with infiltration of neoplastic thyroid follicular cells in regional lymph nodes.
Assuntos
Adenocarcinoma Folicular/veterinária , Camelídeos Americanos , Neoplasias da Glândula Tireoide/veterinária , Adenocarcinoma Folicular/diagnóstico , Adenocarcinoma Folicular/patologia , Animais , Evolução Fatal , Feminino , Saskatchewan , Células Epiteliais da Tireoide/patologia , Glândula Tireoide/patologia , Neoplasias da Glândula Tireoide/diagnóstico , Neoplasias da Glândula Tireoide/patologiaRESUMO
Inflammatory processes, neoplastic growths or rare dysontogenetic malformations may cause mass formation in the gills of fish. In the present case, a follicular carcinoma of the thyroid gland in a Barca snakehead and its surgical removal are reported, and neoplasms in fish are discussed. Following clinical, radiological, cytological and sonographic examinations, the gill-associated partly cystic mass was incompletely removed surgically. The subsequent histological examination identified the mass partly as a follicular carcinoma of the thyroid gland. Because the main alterations of the surgical specimen were non-neoplastic, the development from a rare preexisting hamartoma is discussed. No bacteriological or mycological secondary infections were identified. This report is the first description of a follicular carcinoma and its surgical removal in a snakehead.
Assuntos
Adenocarcinoma Folicular/veterinária , Doenças dos Peixes/cirurgia , Neoplasias da Glândula Tireoide/veterinária , Adenocarcinoma Folicular/cirurgia , Animais , Peixes , Neoplasias da Glândula Tireoide/cirurgiaAssuntos
Adenocarcinoma Folicular/veterinária , Doenças do Cão/patologia , Hiperpigmentação/veterinária , Neoplasias da Glândula Tireoide/veterinária , Adenocarcinoma Folicular/complicações , Adenocarcinoma Folicular/patologia , Animais , Antibacterianos/efeitos adversos , Autopsia/veterinária , Diagnóstico Diferencial , Cães , Hiperpigmentação/induzido quimicamente , Hiperpigmentação/patologia , Coxeadura Animal/etiologia , Masculino , Minociclina/efeitos adversos , Neoplasias da Glândula Tireoide/complicações , Neoplasias da Glândula Tireoide/patologiaRESUMO
An iodide transport defect (ITD) in the thyroid gland was determined to cause congenital dyshormonogenic hypothyroidism with goiter (CDHG) in 2 members of a family of Shih-Tzu dogs. Strikingly, both dogs were also diagnosed with dilated cardiomyopathy at 24 and 1.5 mo of age. The only sign of hypothyroidism was a moderate growth delay in the adult dog. The ITD was recognized by the absence of uptake of technetium-99m in the salivary glands (sg) and goiter observed by scintigraphy. In the same scan, radiopharmaceutical uptake was found in the anterior mediastinum of both dogs and in the right axillary lymph node in the oldest dog. A follicular thyroid carcinoma was diagnosed by histopathology after thyroidectomy of the older dog. An adenomatous goiter with ectopic thyroid tissue, and degenerative changes in myocardium were the findings after necropsy in the youngest dog. A homozygous mutation of the intron 9 splice acceptor site of SLC5A5 gene, encoding the sodium/iodine symporter (NIS), was found in the DNA of one of the affected dogs. The mutation was a single base transition of guanine > adenine (G > A) at position 45,024,672 of dog chromosome 20 (CFA20). Five of eight healthy dogs, including both parents of one of the dogs exhibiting CDHG, were heterozygous A/G, and the other 3 were homozygous for the wild-type allele G/G. No sequence variant was found in thyroid peroxidase of the affected dog. Congenital dyshormonogenic hypothyroidism with goiter in this family is an autosomal recessive trait. Our findings are the first evidence of an SLC5A5 mutation in dogs and establish a new genetic cause of CDHG.
Assuntos
Hipotireoidismo Congênito/veterinária , Doenças do Cão/genética , Bócio/genética , Mutação/genética , Simportadores/genética , Adenocarcinoma Folicular/genética , Adenocarcinoma Folicular/veterinária , Animais , Cardiomiopatias/genética , Cardiomiopatias/veterinária , Hipotireoidismo Congênito/tratamento farmacológico , Hipotireoidismo Congênito/genética , Cães , Heterozigoto , Homozigoto , Terapia de Reposição Hormonal/veterinária , Linhagem , Fenótipo , Glândula Tireoide/diagnóstico por imagemAssuntos
Adenocarcinoma Folicular/veterinária , Doenças do Cão/diagnóstico , Neoplasias da Glândula Tireoide/veterinária , Adenocarcinoma Folicular/complicações , Adenocarcinoma Folicular/diagnóstico , Animais , Biópsia por Agulha Fina/veterinária , Doenças do Cão/diagnóstico por imagem , Doenças do Cão/patologia , Cães , Masculino , Cisto Tireoglosso/complicações , Cisto Tireoglosso/diagnóstico , Cisto Tireoglosso/veterinária , Neoplasias da Glândula Tireoide/complicações , Neoplasias da Glândula Tireoide/diagnóstico , Tomografia Computadorizada por Raios X/veterináriaAssuntos
Adenocarcinoma Folicular/veterinária , Doenças dos Cavalos/patologia , Neoplasias da Glândula Tireoide/veterinária , Adenocarcinoma Folicular/patologia , Adenocarcinoma Folicular/cirurgia , Animais , Biópsia por Agulha Fina/veterinária , Diagnóstico Diferencial , Doenças dos Cavalos/cirurgia , Cavalos , Masculino , Neoplasias da Glândula Tireoide/patologia , Neoplasias da Glândula Tireoide/cirurgiaRESUMO
BACKGROUND: Prognostic markers for dogs with thyroid tumors are limited. HYPOTHESIS/OBJECTIVES: To identify clinical, pathologic, and immunohistochemical prognostic factors for dogs with thyroid tumors. ANIMALS: Seventy dogs with thyroid neoplasia. METHODS: Retrospective study. Dogs with thyroid neoplasia were included when follow-up information and formalin-fixed paraffin-embedded tumor samples were available. Immunohistochemistry (IHC) was performed for thyroglobulin, calcitonin, Ki-67, and E-cadherin. Correlation of tumor variables (diameter, volume, localization, scintigraphic uptake, thyroid function, IHC) with local invasiveness and metastatic disease was performed on all tumor samples. Forty-four dogs treated by thyroidectomy were included in a survival analysis. RESULTS: Fifty dogs (71%) had differentiated follicular cell thyroid carcinoma (dFTC) and 20 (29%) had medullary thyroid carcinoma (MTC). At diagnosis, tumor diameter (P = .007; P = .038), tumor volume (P = .020), tumor fixation (P = .002), ectopic location (P = .002), follicular cell origin (P = .044), and Ki-67 (P = .038) were positively associated with local invasiveness; tumor diameter (P = .002), tumor volume (P = .023), and bilateral location (P = .012) were positively associated with presence of distant metastases. Forty-four dogs (28 dFTC, 16 MTC; stage I-III) underwent thyroidectomy. Outcome was comparable between dogs with dFTC and MTC. Macroscopic (P = .007) and histologic (P = .046) vascular invasion were independent negative predictors for disease-free survival. Although time to presentation, histologic vascular invasion and Ki-67 were negatively associated with time to metastases, and time to presentation was negatively associated with time to recurrence, no independent predictors were found. E-cadherin expression was not associated with outcome. CONCLUSIONS AND CLINICAL IMPORTANCE: Prognostic factors have been identified that provide relevant information for owners and clinicians.
Assuntos
Doenças do Cão/patologia , Neoplasias da Glândula Tireoide/veterinária , Adenocarcinoma Folicular/química , Adenocarcinoma Folicular/diagnóstico , Adenocarcinoma Folicular/mortalidade , Adenocarcinoma Folicular/patologia , Adenocarcinoma Folicular/cirurgia , Adenocarcinoma Folicular/veterinária , Animais , Caderinas/análise , Calcitonina/análise , Carcinoma Neuroendócrino/química , Carcinoma Neuroendócrino/diagnóstico , Carcinoma Neuroendócrino/mortalidade , Carcinoma Neuroendócrino/patologia , Carcinoma Neuroendócrino/cirurgia , Carcinoma Neuroendócrino/veterinária , Doenças do Cão/diagnóstico , Doenças do Cão/mortalidade , Doenças do Cão/cirurgia , Cães , Antígeno Ki-67/análise , Prognóstico , Estudos Retrospectivos , Análise de Sobrevida , Tireoglobulina/análise , Glândula Tireoide/química , Glândula Tireoide/patologia , Neoplasias da Glândula Tireoide/química , Neoplasias da Glândula Tireoide/diagnóstico , Neoplasias da Glândula Tireoide/mortalidade , Neoplasias da Glândula Tireoide/patologia , Neoplasias da Glândula Tireoide/cirurgia , Tireoidectomia/mortalidade , Tireoidectomia/veterináriaRESUMO
BACKGROUND: Information on the genetic events leading to thyroid cancer in dogs is lacking. HYPOTHESIS/OBJECTIVES: Upregulation of the PI3K/Akt pathway has an important role in the tumorigenesis of thyroid carcinoma in dogs. ANIMALS: Fifty-nine dogs with thyroid carcinoma and 10 healthy controls. METHODS: Quantitative RT-PCR was performed for VEGFR-1, VEGFR-2, EGFR, PIK3CA, PIK3CB, PDPK1, PTEN, AKT1, AKT2, COX-2, and CALCA. Mutation analysis was performed for known hotspots of RAS (N, K, H), PIK3CA, BRAF, RET, and for the entire coding region of PTEN. RESULTS: Forty-three dogs (73%) had follicular cell thyroid carcinoma (FTC) and 16 dogs (27%) had medullary thyroid carcinoma (MTC). The relative mRNA expressions of VEGFR-1 (P < .001), VEGFR-2 (P = .002), PDPK1 (P < .001), AKT1 (P = .009), and AKT2 (P < .001) were increased in FTC, and those of EGFR (P < .001), VEGFR-1 (P = .036), and PIK3CA (P = .019) were increased in MTC when compared to normal thyroid glands. Mutation analysis of K-RAS identified 2 activating missense mutations, which also have been described in thyroid cancer of humans. A G12R substitution was present in 1 FTC and an E63K substitution was present in 1 MTC. No functional mutations were found in the sequenced regions of H-RAS, N-RAS, PIK3CA, BRAF, RET, and PTEN. CONCLUSIONS AND CLINICAL IMPORTANCE: The increased expression of several genes associated with PI3K/Akt signaling suggests the involvement of this pathway in the pathogenesis of thyroid carcinoma in dogs, warranting further research on pathway activation and gene amplification. The mutations most frequently associated with thyroid cancer in humans are rare in dogs.
Assuntos
Doenças do Cão/fisiopatologia , Proteína Oncogênica v-akt/fisiologia , Fosfatidilinositol 3-Quinases/fisiologia , Transdução de Sinais/fisiologia , Neoplasias da Glândula Tireoide/veterinária , Adenocarcinoma Folicular/patologia , Adenocarcinoma Folicular/fisiopatologia , Adenocarcinoma Folicular/veterinária , Animais , Carcinoma Neuroendócrino/patologia , Carcinoma Neuroendócrino/fisiopatologia , Carcinoma Neuroendócrino/veterinária , Estudos de Casos e Controles , Doenças do Cão/patologia , Cães , Regulação Neoplásica da Expressão Gênica/fisiologia , Proteína Oncogênica v-akt/biossíntese , Fosfatidilinositol 3-Quinases/biossíntese , Reação em Cadeia da Polimerase/veterinária , Glândula Tireoide/patologia , Neoplasias da Glândula Tireoide/patologia , Neoplasias da Glândula Tireoide/fisiopatologia , Regulação para Cima/fisiologiaRESUMO
A dog developed a cervical mass, and computed tomography verified a mass surrounding the trachea with some pulmonary masses. Histopathologically, the cervical mass was composed of malignant neoplastic cells showing follicular appearance which reacted positive for thyroglobulin on immunohistochemistry. A characteristic feature of the tumor was abundant and metaplastic stromal components. Anastomosed collagenous tissues connecting to capsule of the tumor were abundant in the stroma. In parts of the collagenous tissues, mature cartilages and bones were continuously formed. There was no cellular atypia or invasion in the components. We diagnosed this case as follicular thyroid carcinoma with metaplastic stroma. This is the first case report that characterizes stromal components with chondroid and osseous metaplasia in a canine thyroid carcinoma.
Assuntos
Adenocarcinoma Folicular/veterinária , Osso e Ossos/patologia , Cartilagem/patologia , Doenças do Cão/patologia , Doenças do Cão/cirurgia , Metaplasia/veterinária , Adenocarcinoma Folicular/patologia , Adenocarcinoma Folicular/cirurgia , Animais , Cães , Evolução Fatal , Feminino , Técnicas Histológicas/veterinária , Imuno-Histoquímica/veterinária , Metaplasia/patologia , Tomografia Computadorizada por Raios X/veterináriaRESUMO
BACKGROUND: Thyroid carcinoma is a common endocrine tumor in the dog. Local invasive growth frequently precludes surgical excision and, in up to 38% of dogs, the tumor has already metastasized by the time of diagnosis. Therefore, it is important to investigate new treatment modalities that may be useful for the large number of dogs with inoperable tumors or metastatic disease. HYPOTHESIS/OBJECTIVES: To investigate the immunohistochemical expression of potential therapeutic targets in canine thyroid tumors. ANIMALS: 74 dogs with thyroid neoplasia. METHODS: Immunohistochemistry was performed for thyroglobulin, calcitonin, vascular endothelial growth factor (VEGF), p53, cycloxygenase-2 (cox-2), and P-glycoprotein (P-gp). RESULTS: Fifty-four (73%) tumors were classified as follicular cell thyroid carcinomas (FTCs) and 20 (27%) as medullary thyroid carcinomas (MTCs). Eighty percent of FTCs and all MTCs had a high percentage (76-100%) of neoplastic cells immunopositive for VEGF. Thirteen percent of FTCs and 50% of MTCs expressed cox-2. Seven percent of FTCs and 70% of MTCs expressed P-gp. No tumor was immunopositive for p53 expression. Expression of VEGF (P = .034), cox-2 (P = .013), and P-gp (P < .001) was significantly higher in MTCs compared to FTCs. CONCLUSIONS AND CLINICAL IMPORTANCE: VEGF is a potential therapeutic target in both FTC and MTC in dogs. Cox-2 and P-gp may be useful molecular targets in canine MTC.
Assuntos
Adenocarcinoma Folicular/veterinária , Carcinoma Medular/veterinária , Doenças do Cão/metabolismo , Neoplasias da Glândula Tireoide/veterinária , Membro 1 da Subfamília B de Cassetes de Ligação de ATP/metabolismo , Adenocarcinoma Folicular/metabolismo , Animais , Carcinoma Medular/metabolismo , Ciclo-Oxigenase 2/metabolismo , Cães , Neoplasias da Glândula Tireoide/metabolismo , Proteína Supressora de Tumor p53/metabolismo , Fator A de Crescimento do Endotélio Vascular/metabolismoRESUMO
Follicular thyroid carcinoma (FTC) is an aggressive tumour in dogs with little known about its molecular pathogenesis. The overall goal of this study was to examine FTC and normal thyroid tissue gene expression. Microarray analysis was performed on a pilot group of five FTC-affected dogs and four healthy dogs, and then osteopontin validated with quantitative polymerase chain reaction (PCR) and immunohistochemistry (IHC) of thyroid tissue from non-invasive FTC, invasive FTC and healthy dogs. On microarray analysis, 489 transcripts were differentially expressed between FTC and normal thyroid: 242 transcripts were down-regulated and 247 were up-regulated. Osteopontin expression was markedly increased in tumour tissue compared to normal thyroid tissue. Quantitative PCR and IHC confirmed differential expression of osteopontin in both tumour types (invasive and non-invasive) compared to normal thyroid tissue. There is justification for further investigation of osteopontin as a potential molecular marker for screening and monitoring of canine FTC.
Assuntos
Adenocarcinoma Folicular/veterinária , Doenças do Cão/metabolismo , Perfilação da Expressão Gênica , Regulação Neoplásica da Expressão Gênica/fisiologia , Osteopontina/metabolismo , Neoplasias da Glândula Tireoide/veterinária , Adenocarcinoma Folicular/metabolismo , Adenocarcinoma Folicular/patologia , Animais , Estudos de Casos e Controles , Doenças do Cão/patologia , Cães , Regulação para Baixo , Feminino , Imuno-Histoquímica/veterinária , Masculino , Análise em Microsséries , Osteopontina/genética , Projetos Piloto , Reação em Cadeia da Polimerase/veterinária , RNA/genética , RNA/metabolismo , Neoplasias da Glândula Tireoide/metabolismo , Neoplasias da Glândula Tireoide/patologia , Regulação para CimaRESUMO
An adult male barred owl (Strix varia) was found unable to fly on a pasture during the day. On presentation, several lacerations were noted on the left wing. The animal was anesthetized for radiographic examination, which revealed mild swelling and irregularity of the soft tissues of the left wing. Over the plane of the syrinx and great vessels, ill-defined soft tissue opacity was present. The anesthetic recovery was unsuccessful, and the patient died. On gross necropsy, a 1 cm in diameter, round, soft, red-tan nodule, with scattered light tan to white foci was noticed between the right subclavian artery and the syrinx. The histopathology of this structure was characteristic of a thyroid follicular carcinoma. Neoplastic cells were immunoreactive to thyroglobulin and pancytokeratin proteins. A blood sample, taken antemortem, was analyzed for total and free thyroxine. Due to the lack of reference intervals for the current species, 4 blood samples from other barred owls were taken, 2 of which were clinically normal and 2 with an unhealthy status. The thyroid values were higher than the controls (total thyroxine by radioimmunoassay [µg/dl] 1.1 vs. <0.2, <0.2, 0.6, <0.2; free thyroxine by equilibrium dialysis [ng/dl] >10 vs. <0.3, <0.3, 2.1, <0.3). Although the other 4 birds are not intended to serve as a reference interval because of the low number and unhealthy status, findings are indicative of a productive thyroid follicular carcinoma.
Assuntos
Adenocarcinoma Folicular/veterinária , Doenças das Aves/patologia , Estrigiformes , Neoplasias da Glândula Tireoide/veterinária , Adenocarcinoma Folicular/patologia , Animais , Masculino , Neoplasias da Glândula Tireoide/patologiaRESUMO
A 5-year-old male castrated ferret was presented to the Washington State University College of Veterinary Medicine for evaluation of progressive hair loss and a large, rapidly growing ventral neck mass. The patient had been diagnosed previously with an insulinoma, which was managed medically. Fine-needle aspirates of the neck mass were performed. The cytologic results were most consistent with epithelial neoplasia, likely a carcinoma; thyroid origin was considered likely based on tumor location and cell morphology. The tumor grew rapidly, and the owners elected euthanasia 1 week after examination. At necropsy, a circumscribed, ovoid mass disrupted the right cervical musculature next to the right lobe of the thyroid gland. Histopathologic evaluation revealed an infiltrative mass consisting of cuboidal cells arranged in solid sheets and irregular follicles enclosing colloid. The cells were large, with prominent nucleoli, and had a high mitotic rate. The histopathologic diagnosis was consistent with thyroid follicular adenocarcinoma. Immunochemical findings confirmed thyroglobulin production by neoplastic cells, but to a lesser extent than in normal ferret thyroid tissue. To our knowledge, this is the first case of thyroid follicular adenocarcinoma to be reported in a ferret, with only 1 other case of thyroid carcinoma, a C-cell carcinoma, described previously.