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1.
Opt Express ; 30(2): 2424-2437, 2022 Jan 17.
Artigo em Inglês | MEDLINE | ID: mdl-35209383

RESUMO

A powerful and convenient method for measuring three-dimensional (3D) deformation of moving amoeboid cells will assist the progress of environmental and cytological studies as protists amoebae play a role in the fundamental environmental ecosystem. Here we develop an inexpensive and useful method for measuring 3D deformation of single protists amoeba through binocular microscopy and a newly proposed algorithm of stereo-scopy. From the movies taken from the left and right optical tubes of the binocular microscope, we detect the 3D positions of many intrinsic intracellular vesicles and reconstruct cellular surfaces of amoeboid cells in 3D space. Some observations of sampled behaviors are shown in a single-celled organism of Amoeba proteus. The resultant surface time series is then analyzed to obtain surface velocity, curvature and volume increasing rates of pseudo-pods for characterizing the movements of amoeboid cells. The limitations and errors of this method are also discussed.


Assuntos
Amoeba/citologia , Imageamento Tridimensional/métodos , Microscopia/instrumentação , Visão Binocular/fisiologia
2.
Methods Mol Biol ; 2215: 49-82, 2021.
Artigo em Inglês | MEDLINE | ID: mdl-33367999

RESUMO

Cryo-electron tomography (cryo-ET) is a powerful technique to examine cellular structures as they exist in situ. However, direct imaging by TEM for cryo-ET is limited to specimens up to ∼400 nm in thickness, narrowing its applicability to areas such as cellular projections or small bacteria and viruses. Cryo-focused ion beam (cryo-FIB) milling has emerged in recent years as a method to generate thin specimens from cellular samples in preparation for cryo-ET. In this technique, specimens are thinned with a beam of gallium ions to gradually ablate cellular material in order to leave a thin, electron-transparent section (a lamella) through the bulk material. The lamella can be used for high-resolution cryo-ET to visualize cells in 3D in a near-native state. This approach has proved to be robust and relatively simple for new users and exhibits minimal sectioning artifacts. In this chapter, we describe a general approach to cryo-FIB milling for users with prior cryo-EM experience, with extensive notes on operation and troubleshooting.


Assuntos
Microscopia Crioeletrônica/métodos , Tomografia com Microscopia Eletrônica/métodos , Técnicas de Preparação Histocitológica/instrumentação , Amoeba/citologia , Animais , Bactérias/citologia , Fungos/citologia , Gálio/química , Células HeLa , Técnicas de Preparação Histocitológica/métodos , Humanos , Camundongos , Células NIH 3T3 , Fluxo de Trabalho
3.
Biomech Model Mechanobiol ; 20(1): 167-191, 2021 Feb.
Artigo em Inglês | MEDLINE | ID: mdl-32772275

RESUMO

Amoeboid cells often migrate using pseudopods, which are membrane protrusions that grow, bifurcate, and retract dynamically, resulting in a net cell displacement. Many cells within the human body, such as immune cells, epithelial cells, and even metastatic cancer cells, can migrate using the amoeboid phenotype. Amoeboid motility is a complex and multiscale process, where cell deformation, biochemistry, and cytosolic and extracellular fluid motions are coupled. Furthermore, the extracellular matrix (ECM) provides a confined, complex, and heterogeneous environment for the cells to navigate through. Amoeboid cells can migrate without significantly remodeling the ECM using weak or no adhesion, instead utilizing their deformability and the microstructure of the ECM to gain enough traction. While a large volume of work exists on cell motility on 2D substrates, amoeboid motility is 3D in nature. Despite recent progress in modeling cellular motility in 3D, there is a lack of systematic evaluations of the role of ECM microstructure, cell deformability, and adhesion on 3D motility. To fill this knowledge gap, here we present a multiscale, multiphysics modeling study of amoeboid motility through 3D-idealized ECM. The model is a coupled fluid‒structure and coarse-grain biochemistry interaction model that accounts for large deformation of cells, pseudopod dynamics, cytoplasmic and extracellular fluid motion, stochastic dynamics of cell-ECM adhesion, and microstructural (pore-scale) geometric details of the ECM. The key finding of the study is that cell deformation and matrix porosity strongly influence amoeboid motility, while weak adhesion and microscale structural details of the ECM have secondary but subtle effects.


Assuntos
Amoeba/citologia , Movimento Celular , Junções Célula-Matriz/metabolismo , Matriz Extracelular/metabolismo , Adesão Celular , Núcleo Celular/metabolismo , Simulação por Computador , Modelos Biológicos , Análise Numérica Assistida por Computador , Fatores de Tempo
4.
Protist ; 171(6): 125770, 2020 12.
Artigo em Inglês | MEDLINE | ID: mdl-33166717

RESUMO

Dziani Dzaha is a hypersaline lake (Mayotte island), whose microbial community is dominated by photosynthetic microorganisms. Here, we describe two new free-living heteroloboseans. One belonging to the Pharyngomonas genus and the other, whose 18S rRNA gene sequence shares only 85% homology to its closest relatives Euplaesiobystra hypersalinica, was proposed as a new species of this genus being called Euplaesiobystra dzianiensis. Both strains were salt tolerant to 75‰ and grew between 25 and 37°C. Their distribution patterns varied seasonally and depended also on depth. Noticeably, both free-living amoebae isolates were able to graze on Arthrospira filaments, which are found within the same water layer. In conclusion, we document for the first time the presence and ecology of free-living amoebae in the thalassohaline lake Dziani Dzaha, and describe a new species of the Euplaesiobystra genus.


Assuntos
Amoeba/classificação , Amoeba/citologia , Lagos/parasitologia , Amoeba/genética , Lagos/química , RNA Ribossômico 18S/genética , Tolerância ao Sal/fisiologia , Estações do Ano , Especificidade da Espécie
5.
Protist ; 171(6): 125773, 2020 12.
Artigo em Inglês | MEDLINE | ID: mdl-33243724

RESUMO

The marine protozoan parasite Neoparamoeba perurans has been established as the causative agent for amoebic gill disease (AGD) in Atlantic salmon (Salmo salar). Freshwater bathing is the only routinely used treatment for AGD in Australia while hydrogen peroxide (H2O2) is used in countries with cooler water temperatures. The identification of new treatments that do not rely on either freshwater or H2O2 bathing is highly sought. However, in vitro based methods for high throughput screening of antiparasitic compounds have not been established for this parasite. To this end the present study evaluated two in vitro bioassays based on metabolic energy production and cellular membrane integrity to distinguish between amoebistatic (crenated or pseudocyst forms with recovery possible) and amoebicidal (death) activity. Amoebae were subject to either freshwater, H2O2 or chloramine-T for 4h treatment and assessed 24h after recovery. Visualization by microscopy and bioassay assessment 24h post-treatment confirmed that H2O2 and freshwater are 95% amoebicidal albeit due to different mechanisms of action. These data are consistent with other studies where amoebae have been observed to recover following exposure to these compounds and provide evidence for the inclusion of a recovery component to differentiate between the mechanism of action of amoebicidal and amoebistatic treatments. Together these bioassays are a critical tool for high throughput screening of novel and more effective treatments against AGD.


Assuntos
Amebíase/parasitologia , Amoeba/fisiologia , Bioensaio/normas , Doenças dos Peixes/parasitologia , Ensaios de Triagem em Larga Escala/métodos , Amoeba/citologia , Animais , Organismos Aquáticos , Pesqueiros , Ensaios de Triagem em Larga Escala/normas , Viabilidade Microbiana
6.
Eur J Protistol ; 74: 125691, 2020 Jun.
Artigo em Inglês | MEDLINE | ID: mdl-32200034

RESUMO

We performed karyotyping of Amoeba sp. strain Cont. Based on the results of a cytological analysis, we concluded that the chromosome number of Amoeba sp. strain Cont in mitosis was unstable. In all cases they appeared to be hypergaploid (the basic chromosome number is 30), with monosomy of all chromosomes except four shortest ones. The presence of "extrachromosomes" in the nucleus could prolong until the beginning of the anaphase. It was only then that they were ejected from the nucleus and the euploidy (haploidy) was restored. The stage of endoprophase nucleus was revealed in the cell cycle of Amoeba sp. strain Cont. This stage has not yet been found in other amoebae from the "proteus-type" group that had been previously studied (A. proteus strain B and A. borokensis). The maximum number of endoreplication rounds in the strain Cont amoebae nuclear cycle was 4 or 5. The regular extrusion of chromosomes from the nucleus into the cytoplasm occurred in each of the endoreplication rounds. Comparative cytological analysis of A. proteus strain B, A. borokensis and Amoeba sp. strain Cont karyotypes indicated that strain Cont, though rather close to the former two amoebae, is actually a distinct species.


Assuntos
Amoeba/citologia , Amoeba/genética , Instabilidade Cromossômica/genética , Cariótipo , Mitose/genética , Especificidade da Espécie
7.
PLoS Negl Trop Dis ; 13(10): e0007742, 2019 10.
Artigo em Inglês | MEDLINE | ID: mdl-31589617

RESUMO

Paracoccidioides spp. are thermodimorphic fungi that cause a neglected tropical disease (paracoccidioidomycosis) that is endemic to Latin America. These fungi inhabit the soil, where they live as saprophytes with no need for a mammalian host to complete their life cycle. Despite this, they developed sophisticated virulence attributes allowing them not only to survive in host tissues but also to cause disease. A hypothesis for selective pressures driving the emergence or maintenance of virulence of soil fungi is their interaction with soil predators such as amoebae and helminths. We evaluated the presence of environmental amoeboid predators in soil from armadillo burrows where Paracoccidioides had been previously detected and tested if the interaction of Paracoccidioides with amoebae selects for fungi with increased virulence. Nematodes, ciliates, and amoebae-all potential predators of fungi-grew in cultures from soil samples. Microscopical observation and ITS sequencing identified the amoebae as Acanthamoeba spp, Allovahlkampfia spelaea, and Vermamoeba vermiformis. These three amoebae efficiently ingested, killed and digested Paracoccidioides spp. yeast cells, as did laboratory adapted axenic Acanthamoeba castellanii. Sequential co-cultivation of Paracoccidioides with A. castellanii selected for phenotypical traits related to the survival of the fungus within a natural predator as well as in murine macrophages and in vivo (Galleria mellonella and mice). These changes in virulence were linked to the accumulation of cell wall alpha-glucans, polysaccharides that mask recognition of fungal molecular patterns by host pattern recognition receptors. Altogether, our results indicate that Paracoccidioides inhabits a complex environment with multiple amoeboid predators that can exert selective pressure to guide the evolution of virulence traits.


Assuntos
Amoeba/fisiologia , Interações Hospedeiro-Patógeno/fisiologia , Paracoccidioides/fisiologia , Microbiologia do Solo , Acanthamoeba castellanii/fisiologia , Amoeba/citologia , Amoeba/microbiologia , Animais , Tatus , Cilióforos , Técnicas de Cocultura , Modelos Animais de Doenças , Fungos , Macrófagos/microbiologia , Masculino , Camundongos , Camundongos Endogâmicos BALB C , Nematoides , Paracoccidioides/patogenicidade , Paracoccidioidomicose/microbiologia , Fagocitose , Solo , Virulência , Fatores de Virulência/fisiologia
8.
Biocontrol Sci ; 24(1): 39-45, 2019.
Artigo em Inglês | MEDLINE | ID: mdl-30880312

RESUMO

 Legionella spp. exist naturally in association with amoeba in water environments and are known to be the etiological agent of a severe form of pneumonia. To detect diverse Legionella populations in cooling tower water systems, amoebic coculturing was performed for 15 water samples obtained from five different kinds of facilities in six geographically different locations. The growth of Legionella in coculture with Acanthamoeba sp. cells was monitored by quantitative PCR targeting Legionella-specific 16S rRNA genes. Seven out of the 15 samples were positive for Legionella growth and subjected to clone library analysis. A total of 333 clones were classified into 14 operational taxonomic units composed of seven known species and seven previously undescribed groups. Four of the seven Legionella-growth-positive samples harbored detectable levels of free-living amoeba and were predominated by either L. drozanskii or L. lytica, by both L. bozemanii and L. longbeachae, or by a not-yet-described group named OTU 4. The Legionella-growth- positive samples contained higher ATP levels (>980 pM) than the growth-negative samples (<160 pM) , suggesting that ATP content would be a good indicator of the presence of viable but nonculturable Legionella populations able to grow with amoeba.


Assuntos
Amoeba/citologia , Microbiologia Industrial/normas , Legionella/classificação , Microbiologia da Água/normas , Trifosfato de Adenosina/análise , Técnicas de Cocultura , Legionella/citologia , Legionella/genética , Filogenia , RNA Ribossômico 16S/genética , Reação em Cadeia da Polimerase em Tempo Real , Estações do Ano
9.
Sci Rep ; 9(1): 183, 2019 01 17.
Artigo em Inglês | MEDLINE | ID: mdl-30655573

RESUMO

The discovery of giant viruses in the last years has fascinated the scientific community due to virus particles size and genome complexity. Among such fantastic discoveries, we have recently described tupanviruses, which particles present a long tail, and has a genome that contains the most complete set of translation-related genes ever reported in the known virosphere. Here we describe a new kind of virus-host interaction involving tupanvirus. We observed that tupanvirus-infected amoebas were induced to aggregate with uninfected cells, promoting viral dissemination and forming giant host cell bunches. Even after mechanical breakdown of bunches, amoebas reaggregated within a few minutes. This remarkable interaction between infected and uninfected cells seems to be promoted by the expression of a mannose receptor gene. Our investigations demonstrate that the pre-treatment of amoebas with free mannose inhibits the formation of bunches, in a concentration-dependent manner, suggesting that amoebal-bunch formation correlates with mannose receptor gene expression. Finally, our data suggest that bunch-forming cells are able to interact with uninfected cells promoting the dissemination and increase of tupanvirus progeny.


Assuntos
Amoeba/virologia , Agregação Celular/efeitos dos fármacos , Vírus Gigantes/patogenicidade , Interações Hospedeiro-Patógeno , Viroses/transmissão , Amoeba/citologia , Vírus Gigantes/genética , Lectinas Tipo C/metabolismo , Manose/farmacologia , Receptor de Manose , Lectinas de Ligação a Manose/metabolismo , Receptores de Superfície Celular/metabolismo
10.
Biophys J ; 115(7): 1316-1329, 2018 10 02.
Artigo em Inglês | MEDLINE | ID: mdl-30177444

RESUMO

Recently, it has been reported that the cells of the immune system, as well as Dictyostelium amoebae, can swim in a bulk fluid by changing their shape repeatedly. We refer to this motion as amoeboid swimming. Here, we explore how the propulsion and the deformation of the cell emerge as an interplay between the active forces that the cell employs to activate the shape changes and the passive, viscoelastic response of the cell membrane, the cytoskeleton, and the surrounding environment. We introduce a model in which the cell is represented by an elastic capsule enclosing a viscous liquid. The motion of the cell is activated by time-dependent forces distributed along its surface. The model is solved numerically using the boundary integral formulation. The cell can swim in a fluid medium using cyclic deformations or strokes. We measure the swimming velocity of the cell as a function of the force amplitude, the stroke frequency, and the viscoelastic properties of the cell and the medium. We show that an increase in the shear modulus leads both to a regular slowdown of the swimming, which is more pronounced for more deflated swimmers, and to a tendency toward cell buckling. For a given stroke frequency, the swimming velocity shows a quadratic dependence on force amplitude for small forces, as expected, but saturates for large forces. We propose a scaling relationship for the dependence of swimming velocity on the relevant parameters that qualitatively reproduces the numerical results and allows us to define regimes in which the cell motility is dominated by elastic response or by the effective cortex viscosity. This leads to an estimate of the effective cortex viscosity of 103 Pa ⋅ s for which the two effects are comparable, which is close to that provided by several experiments.


Assuntos
Amoeba/citologia , Amoeba/fisiologia , Citoesqueleto/metabolismo , Elasticidade , Modelos Biológicos , Fenômenos Biomecânicos , Natação
11.
Protoplasma ; 255(2): 517-525, 2018 Mar.
Artigo em Inglês | MEDLINE | ID: mdl-28914376

RESUMO

Cubic membranes (CM) are highly organized membrane structures found in biological systems. They are mathematically well defined and reveal a three-dimensional nano-periodic structure with cubic symmetry. These membrane arrangements are frequently induced in cells under stress, disease conditions, or upon viral infection. In this study, we investigated CM formation in the mitochondria of amoeba Chaos carolinense and observed a striking correlation between the organism's ability to generate CM and the cell survival under starvation. Since starvation also induces autophagy, rapamycin was used to pharmacologically induce autophagy, and interestingly, CM formation was observed in parallel. Conversely, inhibition of autophagy reverted the cubic mitochondrial inner membrane morphology to tubular structure. In starved Chaos cells, mitochondria and autophagosomes did not co-localize and ATP production was sustained. CM transition in the mitochondria during starvation or upon induction of autophagy might prevent their sequestration by autophagosomes, thus slowing their rate of degradation. Such sustained mitochondrial activity may allow amoeba Chaos cells to survive for a longer period upon starvation.


Assuntos
Amoeba/citologia , Membrana Celular/metabolismo , Estresse Fisiológico , Amoeba/metabolismo , Amoeba/ultraestrutura , Autofagossomos/metabolismo , Autofagossomos/ultraestrutura , Autofagia , Sobrevivência Celular , Mitocôndrias/metabolismo , Mitocôndrias/ultraestrutura , Proteínas Mitocondriais/metabolismo , Inanição
13.
Sci Rep ; 7(1): 12675, 2017 10 04.
Artigo em Inglês | MEDLINE | ID: mdl-28978932

RESUMO

Dictyostelium discoideum amoeba is a well-established model organism for studying the crawling locomotion of eukaryotic cells. These amoebae extend pseudopodium - a temporary actin-based protrusion of their body membrane to probe the medium and crawl through it. Experiments show highly-ordered patterns in the growth direction of these pseudopodia, which results in persistence cell motility. Here, we propose a discrete model for studying and investigating the cell locomotion based on the experimental evidences. According to our model, Dictyostelium selects its pseudopodium growth direction based on a second-order Markov chain process, in the absence of external cues. Consequently, compared to a random walk process, our model indicates stronger growth in the mean-square displacement of cells, which is consistent with empirical findings. In the presence of external chemical stimulants, cells tend to align with the gradient of chemoattractant molecules. To quantify this tendency, we define a coupling coefficient between the pseudopodium extension direction and the gradient of an external stimulant, which depends on the local stimulant concentration and its gradient. Additionally, we generalize the model to weak-coupling regime by utilizing perturbation methods.


Assuntos
Amoeba/citologia , Amoeba/crescimento & desenvolvimento , Quimiotaxia , Dictyostelium/citologia , Dictyostelium/crescimento & desenvolvimento , Locomoção , Pseudópodes/metabolismo , Difusão , Modelos Biológicos
14.
J Neuroimmunol ; 310: 143-149, 2017 09 15.
Artigo em Inglês | MEDLINE | ID: mdl-28606377

RESUMO

Microglia provide immune surveillance within the brain and spinal cord. Various microglial morphologies include ramified, amoeboid, and pseudopodic. The link between form and function is not clear, especially for human adult microglia which are limited in availability for study. Here, we examined primary human microglia isolated from normal-appearing white matter. Pseudopodic and amoeboid microglia were effective phagocytes, taking up E. coli bioparticles using ruffled cell membrane sheets and retrograde transport. Pseudopodic and amoeboid microglia were more effective phagocytes as compared to ramified microglia or monocyte-derived dendritic cells. Thus, amoeboid and pseudopodic microglia may both be effective as brain scavengers.


Assuntos
Amoeba/citologia , Microglia/fisiologia , Fagócitos/citologia , Fagócitos/fisiologia , Imagem com Lapso de Tempo , Actinas/metabolismo , Antígenos CD/metabolismo , Antígenos de Diferenciação Mielomonocítica/metabolismo , Infecções Bacterianas , Proteínas de Ligação ao Cálcio , Células Cultivadas , Proteínas de Ligação a DNA/metabolismo , Epilepsia/patologia , Escherichia coli/patogenicidade , Humanos , Proteínas dos Microfilamentos , Microglia/microbiologia , Microglia/patologia , Lobo Temporal/patologia , Fatores de Tempo
15.
Biotechnol Prog ; 33(5): 1418-1424, 2017 Sep.
Artigo em Inglês | MEDLINE | ID: mdl-28593695

RESUMO

Cell density and morphology changes were tested to examine the effects of salts including NaHCO3 , NaCl, KHCO3 , and KCl at 160 mM on protozoa. It was demonstrated that ionic stress rather than osmotic stress led to protozoa cell death and NaHCO3 was shown to be the most effective inhibitor. Deformation of cells and cell shrinkage were observed when protozoan cells were exposed to polyethylene glycol (PEG) or any of the salts. However, while PEG treated cells could fully recover in both number and size, only a small portion of the salt-treated cells survive and cell size was 36-58% smaller than the regular. The disappearance of salt-treated protozoa cells was hypothetically attributed to disruption of the cytoplasmic membrane of these cells. It is further hypothesized that the PEG-treated protozoan cells carried out regulatory volume increase (RVI) after the osmotic shock but the RVI of salt-treated protozoa was hurdled to varied extents. © 2017 American Institute of Chemical Engineers Biotechnol. Prog., 33:1418-1424, 2017.


Assuntos
Amoeba/efeitos dos fármacos , Incrustação Biológica/prevenção & controle , Clorófitas/fisiologia , Cilióforos/efeitos dos fármacos , Pressão Osmótica/efeitos dos fármacos , Sais/farmacologia , Amoeba/citologia , Amoeba/fisiologia , Técnicas de Cultura de Células/normas , Proliferação de Células/efeitos dos fármacos , Forma Celular/efeitos dos fármacos , Clorófitas/citologia , Cilióforos/citologia , Cilióforos/fisiologia , Concentração Osmolar , Polietilenoglicóis/farmacologia
16.
Bioelectromagnetics ; 38(6): 482-493, 2017 Sep.
Artigo em Inglês | MEDLINE | ID: mdl-28543319

RESUMO

The application of physiological strength electric fields may produce a wide range of effects on cells. The mechanisms by which cells detect the presence of these fields, however, are not fully understood. Previous experiments have shown that directionality of cells in the field is governed by an electromechanical mechanism in which the field exerts a torque on the negatively charged, inner glycocalyx that is then transmitted as a force on the cytoskeleton. This mechanism is similar to that by which cells detect fluid shear forces. Several authors, however, have reported that cell directionality and motility behave differently in an electric field. We propose here a second electromechanical mechanism in which the field bends the negatively charged, outer glycocalyx in proximity to the substrate, increasing cell adhesion and, thus, cell motility. The increase in motility depends not only on the field strength, but also on the adhesion of the cell to the substrate prior to application of the field. We show that these mechanisms are common to both human cells and amoebae and, hence, are evolutionarily conserved. Furthermore, the mechanism for detection of electric fields is simply an extension of the mechanism for detecting fluid shears. Bioelectromagnetics. 38:482-493, 2017. © 2017 Wiley Periodicals, Inc.


Assuntos
Movimento Celular , Eletricidade , Glicocálix/metabolismo , Fenômenos Mecânicos , Amoeba/citologia , Fenômenos Biomecânicos , Modelos Biológicos
17.
Plant J ; 90(2): 221-234, 2017 Apr.
Artigo em Inglês | MEDLINE | ID: mdl-28182317

RESUMO

Plastid evolution has been attributed to a single primary endosymbiotic event that occurred about 1.6 billion years ago (BYA) in which a cyanobacterium was engulfed and retained by a eukaryotic cell, although early steps in plastid integration are poorly understood. The photosynthetic amoeba Paulinella chromatophora represents a unique model for the study of plastid evolution because it contains cyanobacterium-derived photosynthetic organelles termed 'chromatophores' that originated relatively recently (0.09-0.14 BYA). The chromatophore genome is about a third the size of the genome of closely related cyanobacteria, but 10-fold larger than most plastid genomes. Several genes have been transferred from the chromatophore genome to the host nuclear genome through endosymbiotic gene transfer (EGT). Some EGT-derived proteins could be imported into chromatophores for function. Two photosynthesis-related genes (psaI and csos4A) are encoded by both the nuclear and chromatophore genomes, suggesting that EGT in Paulinella chromatophora is ongoing. Many EGT-derived genes encode proteins that function in photosynthesis and photoprotection, including an expanded family of high-light-inducible (ncHLI) proteins. Cyanobacterial hli genes are high-light induced and required for cell viability under excess light. We examined the impact of light on Paulinella chromatophora and found that this organism is light sensitive and lacks light-induced transcriptional regulation of chromatophore genes and most EGT-derived nuclear genes. However, several ncHLI genes have reestablished light-dependent regulation, which appears analogous to what is observed in cyanobacteria. We postulate that expansion of the ncHLI gene family and its regulation may reflect the light/oxidative stress experienced by Paulinella chromatophora as a consequence of the as yet incomplete integration of host and chromatophore metabolisms.


Assuntos
Amoeba/citologia , Amoeba/metabolismo , Cromatóforos/metabolismo , Luz , Estresse Oxidativo/efeitos da radiação , Fotossíntese/genética , Fotossíntese/fisiologia , Plastídeos/metabolismo , Simbiose/efeitos da radiação
18.
J Eukaryot Microbiol ; 64(2): 257-265, 2017 03.
Artigo em Inglês | MEDLINE | ID: mdl-27543384

RESUMO

Since the first environmental DNA surveys, entire groups of sequences called "environmental clades" did not have any cultured representative. LKM74 is an amoebozoan clade affiliated to Dermamoebidae, whose presence is pervasively reported in soil and freshwater. We obtained an isolate from soil that we assigned to LKM74 by molecular phylogeny, close related to freshwater clones. We described Mycamoeba gemmipara based on observations made with light- and transmission electron microscopy. It is an extremely small amoeba with typical lingulate shape. Unlike other Dermamoebidae, it lacked ornamentation on its cell membrane, and condensed chromatin formed characteristic patterns in the nucleus. M. gemmipara displayed a unique life cycle: trophozoites formed walled coccoid stages which grew through successive buddings and developed into branched structures holding cysts. These structures, measuring hundreds of micrometres, are built as the exclusive product of osmotrophic feeding. To demonstrate that M. gemmipara is a genuine soil inhabitant, we screened its presence in an environmental soil DNA diversity survey performed on an experimental setup where pig cadavers were left to decompose in soils to follow changes in eukaryotic communities. Mycamoeba gemmipara was present in all samples, although related reads were uncommon underneath the cadaver.


Assuntos
Amebozoários/classificação , Amebozoários/crescimento & desenvolvimento , Meio Ambiente , Estágios do Ciclo de Vida , Filogenia , Amoeba/classificação , Amoeba/citologia , Amebozoários/genética , Amebozoários/ultraestrutura , Animais , Biodiversidade , Cadáver , Membrana Celular , DNA de Protozoário/genética , Água Doce/parasitologia , Sequenciamento de Nucleotídeos em Larga Escala/métodos , Microscopia Eletrônica de Transmissão , Organelas/ultraestrutura , RNA Ribossômico 18S/genética , Solo/parasitologia , Suínos/parasitologia , Suíça , Trofozoítos/crescimento & desenvolvimento
19.
Eur J Protistol ; 56: 180-190, 2016 Oct.
Artigo em Inglês | MEDLINE | ID: mdl-27682890

RESUMO

Planktonic testate amoebae in floodplains exhibit a broad-range of morphological variability. The variation size is already known, but it is necessary to know how this is for morphological variables. This study aimed to identify the relationships between testate amoebae morphology and environmental factors in four neotropical floodplains. We conducted detailed morphometric analyses on 27 common species of planktonic testate amoebae from genera Arcella, Centropyxis, Cucurbitella, Suiadifflugia, Difflugia, Lesquereusia and Netzelia. We sampled subsurface water from each lake in 72 lakes in four Brazilian floodplain lakes. Our goals were to assess: (1) the range of their morphological variability (a) over space within each floodplain, and (b) among the four floodplains, and (c) over time, and (2) which environmental factors explained this variation. Mean shell height and breadth varied considerably among the different floodplain lakes, especially in the Pantanal and Amazonian floodplains. The morphological variability of testate amoeba was correlated to environmental conditions (ammonia, nitrate, phosphate, chlorophyll-a, turbidity, temperature, and depth). Thus, understanding the morphological variation of the testate amoeba species can elucidate many questions involving the ecology of these organisms. Furthermore, could help molecular studies, bioindicator role of these organisations, environmental reconstruction, among others.


Assuntos
Amoeba/citologia , Meio Ambiente , Lagos/parasitologia , Brasil , Clima Tropical
20.
Eur J Protistol ; 56: 119-133, 2016 Oct.
Artigo em Inglês | MEDLINE | ID: mdl-27643668

RESUMO

The diversity of heterolobosean amoebae, important members of soil, marine and freshwater microeukaryote communities in the temperate zones, is greatly under-explored in high latitudes. To address this imbalance, we studied the diversity of this group of free-living amoebae in the Arctic and the Antarctic using culture dependent methods. Eighteen strain representatives of three heterolobosean genera, Allovahlkampfia Walochnik et Mulec, 2009 (1 strain), Vahlkampfia Chatton et Lalung-Bonnaier, 1912 (2) and Naegleria Alexeieff, 1912 (15) were isolated from 179 samples of wet soil and fresh water with sediments collected in 6 localities. The Allovahkampfia strain is the first representative of the genus from the Antarctic; 14 strains (7 from the Arctic, 7 from the Antarctic) of the highly represented genus Naegleria complete the 'polar' cluster of five Naegleria species previously known from the Arctic and Sub-Antarctic regions, whereas one strain enriches the 'dobsoni' cluster of Naegleria strains of diverse origin. Present isolations of Naegleria polarisDe Jonckheere, 2006 from Svalbard, in the Arctic and Vega Island, in the Antarctic and N. neopolarisDe Jonckheere, 2006 from Svalbard and Greenland in the Arctic, and James Ross Island, the Antarctic demonstrate their bipolar distribution, which in free-living amoebae has so far only been known for Vermistella Morand et Anderson, 2007.


Assuntos
Amoeba/classificação , Biodiversidade , Filogenia , Solo/parasitologia , Amoeba/citologia , Amoeba/genética , Amoeba/ultraestrutura , Regiões Antárticas , Regiões Árticas , Variação Genética , RNA Ribossômico 18S/genética , Especificidade da Espécie , Temperatura
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