RESUMO
The effect of UV-light (240-390 nm) in doses of 151 and 755 J/m2 on the expression of membrane markers CD5, CD19, CD20 in human peripheral blood B cells was studied by flow cytometry. In 24 h after exposure to UV light, we observed activation of processes accompanied by structural rearrangements of B-cell membranes leading to changes in the expression of receptor molecules: the content of of CD19 and CD20 increased due to activation of the synthesis of these proteins, while the content of CD5 decreased. The percentage of CD5+ cells decreased over 24 h after UV-irradiation of lymphocytes, while addition of autologous plasma to the incubation medium produced a photoprotective effect on CD5+ cells.
Assuntos
Antígenos CD , Linfócitos B , Transfusão de Sangue Autóloga , Antígenos CD/metabolismo , Antígenos CD/efeitos da radiação , Antígenos CD19/metabolismo , Antígenos CD19/efeitos da radiação , Antígenos CD20/metabolismo , Antígenos CD20/efeitos da radiação , Linfócitos B/metabolismo , Linfócitos B/efeitos da radiação , Biomarcadores/metabolismo , Antígenos CD5/metabolismo , Antígenos CD5/efeitos da radiação , Membrana Celular/metabolismo , Membrana Celular/efeitos da radiação , Humanos , Imunidade Celular/efeitos da radiação , Imunoterapia/métodos , Raios Ultravioleta/efeitos adversosRESUMO
OBJECTIVE: To find effects of low dose radiation (LDR) induced proteins on lymphocyte subsets. METHOD: Stimulative effects of LDR-induced proteins on human peripheral blood lymphocyte subsets were studied. RESULT: The 14C-TdR incorporation in CD4, CD8, CD19 cells increased by 118.23%, 115.18% and 121.62% respectively compared with control group. CONCLUSION: LDR induced proteins can stimulate proliferation of CD4, CD8 and CD19 cells.