RESUMO
BACKGROUND: The frequent use of antibacterial agents and the exposure of the patients to lifesaving intervention processes are consistently associated with the increased chance of nosocomial infections and the emergence of multidrug resistant microorganisms in the hospital environment. Thus, new antimicrobial agents are of unmet need to treat the severe nosocomial infections caused by these putative pathogens resistant to currently available agents. METHOD: Design, synthesis, and biological evaluation of analogues of nitazoxanide (NTZ), an FDA approved thiazolide antiparasitic, as new antimicrobial agents against nosocomial pathogens were described. The NTZ analogues were rationally explored on the basis of either increasing the electronic resonance effects at the nitrothiazolide moiety or improving the anionic form of the whole NTZ structure. RESULTS: The MICs and MBCs values of these NTZ analogues against prevalent nosocomial pathogens were measured. The benzologous analogues 3a and 4a and p-chlorobenzenesulfonamides 8d and 9d exhibited tremendous antimicrobial activities, which were 100- to 2000-fold more potent than NTZ and ciprofloxacin. CONCLUSION: The results demonstrated that delicate manipulation of the NTZ core structure could lead to promising antimicrobial agents against the nosocomial pathogens.
Assuntos
Antibacterianos/farmacologia , Tiazóis/farmacologia , Antibacterianos/síntese química , Ciprofloxacina/farmacologia , Infecção Hospitalar/microbiologia , Bactérias Gram-Negativas/efeitos dos fármacos , Bacilos Gram-Positivos Formadores de Endosporo/efeitos dos fármacos , Testes de Sensibilidade Microbiana , Nitrocompostos , Ftalimidas/síntese química , Ftalimidas/farmacologia , Relação Estrutura-Atividade , Sulfonamidas/síntese química , Sulfonamidas/farmacologia , Tiazóis/síntese químicaRESUMO
Aiming to identify new sources of bioactive secondary metabolites, we isolated 82 endophytic fungi from stems and barks of the native Brazilian tree Caesalpinia echinata Lam. (Fabaceae). We tested their ethyl acetate extracts in several in vitro assays. The organic extracts from three isolates showed antibacterial activity against Staphylococcus aureus and Escherichia coli [minimal inhibitory concentration (MIC) 32-64 µg/mL]. One isolate inhibited the growth of Salmonella typhimurium (MIC 64 µg/mL) and two isolates inhibited the growth of Klebsiella oxytoca (MIC 64 µg/mL), Candida albicans and Candida tropicalis (MIC 64-128 µg/mL). Fourteen extracts at a concentration of 20 µg/mL showed antitumour activities against human breast cancer and human renal cancer cells, while two isolates showed anti-tumour activities against human melanoma cancer cells. Six extracts were able to reduce the proliferation of human peripheral blood mononuclear cells, indicating some degree of selective toxicity. Four isolates were able to inhibit Leishmania (Leishmania) amazonensis and one isolate inhibited Trypanosoma cruzi by at least 40% at 20 µg/mL. The trypanocidal extract obtained from Fusarium sp. [KF611679] culture was subjected to bioguided fractionation, which revealed beauvericin as the compound responsible for the observed toxicity of Fusarium sp. to T. cruzi. This depsipeptide showed a half maximal inhibitory concentration of 1.9 µg/mL (2.43 µM) in a T. cruzi cellular culture assay.
Assuntos
Caesalpinia/microbiologia , Depsipeptídeos/farmacologia , Endófitos/isolamento & purificação , Fusarium/isolamento & purificação , Tripanossomicidas/farmacologia , Trypanosoma cruzi/efeitos dos fármacos , Anti-Infecciosos/isolamento & purificação , Anti-Infecciosos/farmacologia , Candida albicans/efeitos dos fármacos , Linhagem Celular Tumoral/efeitos dos fármacos , Fracionamento Químico , Misturas Complexas , Primers do DNA , Depsipeptídeos/isolamento & purificação , Endófitos/classificação , Enterobacteriaceae/efeitos dos fármacos , Fusarium/metabolismo , Bacilos Gram-Positivos Formadores de Endosporo/efeitos dos fármacos , Humanos , Leishmania/efeitos dos fármacos , Leucócitos Mononucleares/efeitos dos fármacos , Testes de Sensibilidade Microbiana , Pseudomonas aeruginosa/efeitos dos fármacos , Tripanossomicidas/isolamento & purificaçãoRESUMO
Bone grafts are used for reconstructing bone defects caused by implant-associated complications, trauma, and tumors. Surgery with bone allografts is complex and time consuming; therefore, it is prone to a higher infection rate (2.0%-2.5%). In the case of site infection, systemically administered antibiotics cannot reach the infected bone graft. This study evaluated the use of resorbable bone graft substitute powder (HERAFILL; Heraeus Medical GmbH, Wehrheim, Germany) as a bone void-filling material as well as an antibiotic carrier for mixing with bone grafts. The antibiotic activity of the bone chips mixed with HERAFILL powder was measured by drug release tests and bacterial susceptibility with Bacillus subtilis, Staphylococcus epidermidis, and Staphylococcus aureus. HERAFILL powder was added to the bone chips (bone chips/HERAFILL; w/w = 1:1), mixed with a spatula, and vortexed for 1 minute. Gentamicin base release was evaluated in phosphate-buffered saline for up to 7 days using B subtilis bioassay. Antimicrobial efficacy was tested with S aureus and S epidermidis. The average amount of gentamicin base released from bone chips mixed with HERAFILL at 0 to 12 hours was 99.66 mg/mL. On day 7, the gentamicin base released 0.42 mg/mL. The elution released from bone chips mixed with HERAFILL promoted the formation of a zone of inhibition on S epidermidis and S aureus plates. This study confirmed the capacity of bone grafts to act as antibiotic carriers once mixed with HERAFILL powder. Bone chips mixed with HERAFILL showed efficacy against S aureus and S epidermidis.
Assuntos
Antibacterianos/farmacologia , Substitutos Ósseos , Gentamicinas/farmacologia , Bacilos Gram-Positivos Formadores de Endosporo/efeitos dos fármacos , Antibacterianos/administração & dosagem , Bacillus subtilis/efeitos dos fármacos , Substitutos Ósseos/farmacologia , Carbonato de Cálcio , Portadores de Fármacos , Gentamicinas/administração & dosagem , Humanos , Testes de Sensibilidade Microbiana , Pós , Staphylococcus aureus/efeitos dos fármacos , Staphylococcus epidermidis/efeitos dos fármacosRESUMO
The antimicrobial activity of hemoglobin fragments (hemocidins) has been reported in a variety of models. The cattle tick Rhipicephalus (Boophilus) microplus is a blood sucking arthropod from where the first in vivo-generated hemocidin was characterized (Hb 33-61). In the present work we identified a novel antimicrobial peptide from the midgut of fully engorged R. (B.) microplus females, which comprises the amino acids 98-114 of the alpha subunit of bovine hemoglobin, and was designated Hb 98-114. This peptide was active against several yeast and filamentous fungi, although no activity was detected against bacteria up to 50µM of the synthetic peptide. Hb 98-114 was capable of permeabilizing Candida albicans cell membrane and had a fungicidal effect against this yeast. Circular dichroism (CD) and nuclear magnetic resonance (NMR) experiments showed that Hb 98-114 has a random conformation in aqueous solution but switches to an alpha-helical conformation in the presence of sodium dodecyl sulfate (SDS). This alpha helix adopts an amphipathic structure which may be the mechanism of cell membrane permeabilization. Importantly, Hb 98-114 may play an important role in defending the tick midgut against fungal pathogens and is the first hemocidin with specific antifungal activity to be characterized.
Assuntos
Antibacterianos/farmacologia , Antifúngicos/farmacologia , Trato Gastrointestinal/química , Hemoglobinas/farmacologia , Fragmentos de Peptídeos/farmacologia , Rhipicephalus , Sequência de Aminoácidos , Animais , Antibacterianos/química , Antibacterianos/isolamento & purificação , Antifúngicos/química , Antifúngicos/isolamento & purificação , Bovinos , Membrana Celular/efeitos dos fármacos , Membrana Celular/metabolismo , Cromatografia Líquida de Alta Pressão , Cromatografia de Fase Reversa , Enterobacteriaceae/efeitos dos fármacos , Feminino , Corantes Fluorescentes/metabolismo , Bacilos Gram-Positivos Formadores de Endosporo/efeitos dos fármacos , Hemoglobinas/química , Hemoglobinas/isolamento & purificação , Testes de Sensibilidade Microbiana , Fungos Mitospóricos/efeitos dos fármacos , Fungos Mitospóricos/metabolismo , Modelos Moleculares , Dados de Sequência Molecular , Fragmentos de Peptídeos/química , Fragmentos de Peptídeos/isolamento & purificação , Permeabilidade , Estrutura Secundária de Proteína , Pseudomonas aeruginosa/efeitos dos fármacosRESUMO
The aim of the study was to investigate antimicrobial susceptibilities of bovine Helcococcus ovis isolates and to detect genes encoding for H. ovis erythromycin and tetracycline resistance. Twenty-nine isolates were included and the minimal inhibitory concentrations (MICs) of seven antimicrobials were determined using test conditions as given in an approved CLSI guideline for the pyridoxal-dependent Abiotrophia spp. and Granulicatella spp. Furthermore, the macrolide resistance phenotype was examined by the erythromycin-clindamycin double-disk test (D-zone test). Erythromycin MICs of ≥ 8 µg/ml were found in three (10%) isolates which also presented the macrolide, lincosamide, and streptogramin B (MLS(B)) resistance phenotype, either constitutive or inducible. The erm(B) gene was detected in one of these isolates. Increased tetracycline MICs (≥ 8 µg/ml) were obtained for 24 (83%) isolates, mostly associated with the tet(M) gene alone (n=21) or both the tet(L) and tet(M) genes (n=2). The MICs determined for penicillin, ampicillin, amoxicillin-clavulanic acid, and cephalothin did not indicate resistance to these antimicrobials. The study suggests that resistance to MLS(B) antimicrobials and tetracycline is frequent in H. ovis. Moreover, this is the first report about occurrence of the resistance genes erm(B), tet(L), and tet(M) in the Helcococcus genus.
Assuntos
Antibacterianos/farmacologia , Farmacorresistência Bacteriana/genética , Bacilos Gram-Positivos Formadores de Endosporo/efeitos dos fármacos , Bacilos Gram-Positivos Formadores de Endosporo/genética , Animais , Bovinos/microbiologia , Clindamicina/farmacologia , Eritromicina/farmacologia , Lincosamidas/farmacologia , Macrolídeos/farmacologia , Testes de Sensibilidade Microbiana , Fenótipo , Estreptogramina Grupo B/farmacologia , Tetraciclina/farmacologia , Resistência a Tetraciclina/genéticaRESUMO
The inhibitory effects of hinokitiol, a constituent of the woody oils isolated from Cupressaceae heartwood, on mushroom tyrosinase and melanin formation in B16 melanoma cells as well as its antimicrobial activity were investigated. Our results showed that hinokitiol could strongly inhibit both monophenolase activity and diphenolase activity of the enzyme and the inhibition was reversible. The IC(50) values were estimated as 9.67 µM for monophenolase activity and 0.21 µM for diphenolase activity. The lag time of the monophenolase activity was not obviously lengthened by the compound. Kinetic analyses showed that the inhibition mechanism of hinokitiol was a mixed-type inhibition of the diphenolase activity. Hinokitiol effectively inhibited both cellular tyrosinase activity and melanin biosynthesis in B16 melanoma cells with significant cytotoxicity. Furthermore, it was found that hinokitiol could inhibit the proliferation of Salmonella enteritidis, Escherichia coli, Bacillus subtilis, Staphyloccocus aureus, Klebsiella pneumoniae, and Ralstonia solanacearum to different extents. This research may widen the use of hinokitiol in the fields of food preservation, depigmentation, and insecticide use.
Assuntos
Antibacterianos/farmacologia , Inibidores Enzimáticos/farmacologia , Melaninas/biossíntese , Monofenol Mono-Oxigenase/antagonistas & inibidores , Monoterpenos/farmacologia , Tropolona/análogos & derivados , Agaricales/enzimologia , Animais , Linhagem Celular Tumoral , Proliferação de Células/efeitos dos fármacos , Sobrevivência Celular/efeitos dos fármacos , Conservantes de Alimentos/farmacologia , Proteínas Fúngicas/antagonistas & inibidores , Proteínas Fúngicas/metabolismo , Bactérias Gram-Negativas/efeitos dos fármacos , Bacilos Gram-Positivos Formadores de Endosporo/efeitos dos fármacos , Cinética , Melanócitos/efeitos dos fármacos , Melanócitos/enzimologia , Melanoma Experimental , Camundongos , Testes de Sensibilidade Microbiana , Monofenol Mono-Oxigenase/metabolismo , Especificidade por Substrato , Tropolona/farmacologiaRESUMO
Himatanthus articulatus (Vahl) Woodson is a tree found in the northern Amazon savannahs (common name: sucuba) that is used in local Amerindian medicine. Leaf, bark and branch wood methanol extracts, sequentially obtained hexane, ethyl acetate and methanol extracts and latex were evaluated for antifungal and antibacterial activities against American Type Culture Collection (ATCC) and local clinical strains using the disc diffusion method. Methanol extracts and latex inhibited Candida albicans, leaf methanol extracts inhibited Staphylococcus aureus and Bacillus subtilis and bark methanol extracts inhibited B. subtilis. Active extracts inhibited the ATCC and clinical strains. Polar antifungal and antibacterial principles in latex and extracts are thought to be responsible for the inhibition.
Assuntos
Antibacterianos/farmacologia , Antifúngicos/farmacologia , Apocynaceae/química , Candida albicans/efeitos dos fármacos , Escherichia coli/efeitos dos fármacos , Bacilos Gram-Positivos Formadores de Endosporo/efeitos dos fármacos , Antibacterianos/isolamento & purificação , Antifúngicos/isolamento & purificação , Bacillus subtilis/efeitos dos fármacos , Testes de Sensibilidade a Antimicrobianos por Disco-Difusão , Extratos Vegetais/farmacologia , Staphylococcus aureus/efeitos dos fármacosRESUMO
Most research on growing bacterial colonies on agar plates has concerned the effect of genetic or morphotype variation. Some studies have indicated that there is a correlation between microscopic bacterial motion and macroscopic colonial expansion, especially for swarming strains, but no measurements have been obtained for a single strain to relate the microscopic scale to the macroscopic scale. We examined here a single strain (Paenibacillus dendritiformis type T; tip splitting) to determine both the macroscopic growth of colonies and the microscopic bacterial motion within the colonies. Our multiscale measurements for a variety of growth conditions revealed that motion on the microscopic scale and colonial growth are largely independent. Instead, the growth of the colony is strongly affected by the availability of a surfactant that reduces surface tension.
Assuntos
Bacilos Gram-Positivos Formadores de Endosporo/crescimento & desenvolvimento , Bacilos Gram-Positivos Formadores de Endosporo/fisiologia , Tensoativos/metabolismo , Tensoativos/farmacologia , Ágar , Técnicas Bacteriológicas , Contagem de Colônia Microbiana , Meios de Cultura , Bacilos Gram-Positivos Formadores de Endosporo/efeitos dos fármacos , Microscopia de Contraste de Fase , Movimento/efeitos dos fármacosRESUMO
Himatanthus articulatus (Vahl) Woodson is a tree found in the northern Amazon savannahs (common name: sucuba) that is used in local Amerindian medicine. Leaf, bark and branch wood methanol extracts, sequentially obtained hexane, ethyl acetate and methanol extracts and latex were evaluated for antifungal and antibacterial activities against American Type Culture Collection (ATCC) and local clinical strains using the disc diffusion method. Methanol extracts and latex inhibited Candida albicans, leaf methanol extracts inhibited Staphylococcus aureus and Bacillus subtilis and bark methanol extracts inhibited B. subtilis. Active extracts inhibited the ATCC and clinical strains. Polar antifungal and antibacterial principles in latex and extracts are thought to be responsible for the inhibition.
Assuntos
Antibacterianos/farmacologia , Antifúngicos/farmacologia , Apocynaceae/química , Candida albicans/efeitos dos fármacos , Escherichia coli/efeitos dos fármacos , Bacilos Gram-Positivos Formadores de Endosporo/efeitos dos fármacos , Antibacterianos/isolamento & purificação , Antifúngicos/isolamento & purificação , Bacillus subtilis/efeitos dos fármacos , Testes de Sensibilidade a Antimicrobianos por Disco-Difusão , Extratos Vegetais/farmacologia , Staphylococcus aureus/efeitos dos fármacosRESUMO
A study was conducted to determine the effects of three commercially available disinfectants on the reduction of Alicyclobacillus acidoterrestris spores in single-strength apple juice applied to stainless steel surfaces. Apple juice was inoculated with A. acidoterrestris spores, spread onto the surface of stainless steel chips (SSC), dried to obtain spore concentrations of approximately 10(4) CFU/cm2, and treated with disinfectants at temperatures ranging from 40 to 90 degrees C. The concentrations of disinfectants were 200, 500, 1,000, and 2,000 ppm of total chlorine for Clorox (CL) (sodium hypochlorite); 50, 100, and 200 ppm of total chlorine for Carnebon 200 (stabilized chlorine dioxide); and 1,500, 2,000, and 2,600 ppm for Vortexx (VOR) (hydrogen peroxide, peroxyacetic acid, and octanoic acid). For all temperatures tested, VOR at 2,600 ppm (90 degrees C) and CL at 2,000 ppm (90 degrees C) were the most inhibitory against A. acidoterrestris spores, resulting in 2.55- and 2.32-log CFU/cm2 reductions, respectively, after 2 min. All disinfectants and conditions tested resulted in the inactivation of A. acidoterrestris spores, with a maximum reduction of > 2 log CFU/cm2. Results from this study indicate that A. acidoterrestris spores, in single-strength apple juice, may be effectively reduced on stainless steel surface by VOR and CL, which may have practical applications in the juice industry.
Assuntos
Bebidas/microbiologia , Desinfetantes/farmacologia , Conservação de Alimentos/métodos , Bacilos Gram-Positivos Formadores de Endosporo/efeitos dos fármacos , Compostos Clorados/farmacologia , Contagem de Colônia Microbiana , Relação Dose-Resposta a Droga , Microbiologia de Alimentos , Bacilos Gram-Positivos Formadores de Endosporo/crescimento & desenvolvimento , Peróxido de Hidrogênio/farmacologia , Malus/microbiologia , Óxidos/farmacologia , Ácido Peracético/farmacologia , Hipoclorito de Sódio/farmacologia , Esporos Bacterianos/efeitos dos fármacos , Esporos Bacterianos/crescimento & desenvolvimento , Aço Inoxidável , TemperaturaRESUMO
Paenibacillus larvae is the causative agent of American Foulbrood (AFB), a severe disease that affects larvae of the honeybees. Due to the serious effects associated with AFB and the problems related to the use of antibiotics, it is necessary to develop alternative strategies for the control of the disease. The aim of the present work was to evaluate the effect of a propolis ethanolic extract (PEE) against P. larvae and its potential for the control of AFB. In vitro activity of PEE against P. larvae isolates was evaluated by the disk diffusion method and the minimum inhibitory concentration (MIC) was determined. Toxicity for honeybees was evaluated by oral administration of PEE and its lethal concentration was assessed. Lastly, colonies from an apiary with episodes of AFB on previous years were divided into different groups and treated with sugar syrup supplemented with PEE by aspersion (group one), sugar syrup by aspersion (group two), fed with sugar syrup supplemented with PEE (group three) and fed with sugar syrup only (group four). All isolates were sensitive to PEE and the MIC median was 0.52% (range 0.32-0.64). PEE was not toxic for bees at least at 50%. Field assays showed that 21 and 42 days after the application of the treatments, the number of P. larvae spores/g of honey was significantly lower in colonies treated with PEE compared to the colonies that were not treated with PEE. To our knowledge, this is the first report about the use of propolis for the treatment of beehives affected with P. larvae spores.
Assuntos
Antibacterianos/farmacologia , Abelhas/microbiologia , Bacilos Gram-Positivos Formadores de Endosporo/efeitos dos fármacos , Própole/farmacologia , Animais , Antibacterianos/química , Larva/microbiologia , Testes de Sensibilidade Microbiana , Própole/química , Estados UnidosRESUMO
For a long period the thermal processing has been considered as the only way to reduce the initial spore number of Alicyclobacillus acidoterrestris and prevent the spoilage of acidic beverage. New methods, however, were proposed by the literature to control spore germination both in laboratory media and in real systems. After a brief introduction on the impact of A. acidoterrestris in food microbiology and a description of enumeration methods and heat processing applied by the juices manufactures, a review of innovative approaches to inhibit and/or control spore germination is proposed. In particular, this paper focuses on two different topics; the 1st is the use of some natural compounds (monolaurin, lysozyme, nisin and essential oils) or some chemicals, conventional (like sodium-benzoate, organic acids, surfactants and chlorine dioxide) or not conventional (chlorine dioxide as gas). The 2nd topic is a description of some innovative methods to reduce the initial spore number (high hydrostatic and homogenisation pressures, radiation and microwaves).
Assuntos
Contaminação de Alimentos/prevenção & controle , Conservação de Alimentos/métodos , Bacilos Gram-Positivos Formadores de Endosporo/fisiologia , Esporos Bacterianos/crescimento & desenvolvimento , Bebidas/microbiologia , Compostos Clorados/farmacologia , Contagem de Colônia Microbiana , Microbiologia de Alimentos , Bacilos Gram-Positivos Formadores de Endosporo/efeitos dos fármacos , Bacilos Gram-Positivos Formadores de Endosporo/efeitos da radiação , Temperatura Alta , Pressão Hidrostática , Micro-Ondas , Óxidos/farmacologia , Esporos Bacterianos/efeitos dos fármacos , Esporos Bacterianos/efeitos da radiaçãoRESUMO
AIMS: To test the effect of bovicin HC5 against vegetative cells and endospores of Alicyclobacillus acidoterrestris DSMZ 2498 in synthetic media and in acidic mango pulp. METHODS AND RESULTS: Alicyclobacillus acidoterrestris was grown in synthetic medium at 40 degrees C and pH 4.0. The effect on vegetative cells was assayed by adding bovicin HC5 to synthetic medium (40-160 AU ml(-1)) or to mango pulp (100 AU ml(-1)) at various pH values and determining the effect on growth (OD(600 nm)) and viable cell number, respectively. The effect of bovicin HC5 on spore germination and thermal sensitivity of A. acidoterrestris was tested in mango pulp (pH 4.0) containing 80 AU ml(-1) of bovicin HC5. Bovicin HC5 was bactericidal against vegetative cells of A. acidoterrestris at different pH values and showed sporicidal activity against endospores of this bacterium. When spores of A. acidoterrestris were heat treated in the presence of bovicin HC5, D-values decreased 77% to 95% compared to untreated controls at temperatures ranging from 80 to 95 degrees C. CONCLUSION: Bovicin HC5 was bactericidal and sporicidal against A. acidoterrestrsi DSMZ 2498. SIGNIFICANCE AND IMPACT OF THE STUDY: These results indicated that bovicin HC5 has potential to prevent spoilage of acidic fruit juices by thermocidophilic spore-forming bacteria.
Assuntos
Bacteriocinas/farmacologia , Bebidas , Microbiologia de Alimentos , Conservantes de Alimentos/farmacologia , Bacilos Gram-Positivos Formadores de Endosporo/efeitos dos fármacos , Mangifera , Extratos Vegetais , Técnicas Bacteriológicas , Germinação , Bacilos Gram-Positivos Formadores de Endosporo/fisiologia , Temperatura Alta , Esporos Bacterianos/efeitos dos fármacos , Esporos Bacterianos/fisiologiaRESUMO
The honey bee disease American foulbrood (AFB) is a serious problem since its causative agent (Paenibacillus larvae) has become increasingly resistant to conventional antibiotics. The objective of this study was to investigate the in vitro activity of propolis collected from various states of Brazil against P. larvae. Propolis is derived from plant resins collected by honey bees (Apis mellifera) and is globally known for its antimicrobial properties and particularly valued in tropical regions. Tests on the activity of propolis against P. larvae were conducted both in Brazil and Minnesota, USA using two resistance assay methods that measured zones of growth inhibition due to treatment exposure. The propolis extracts from the various states of Brazil showed significant inhibition of P. larvae. Clear dose responses were found for individual propolis extracts, particularly between the concentrations of 1.7 and 0.12 mg propolis/treatment disk, but the source of the propolis, rather than the concentration, may be more influential in determining overall activity. Two of the three tested antibiotics (tylosin and terramycin) exhibited a greater level of inhibition compared to most of the Brazilian samples, which could be due to the low concentrations of active compounds present in the propolis extracts. Additionally, the majority of the Brazilian propolis samples were more effective than the few collected in MN, USA. Due to the evolution of resistance of P. larvae to conventional antibiotic treatments, this research is an important first step in identifying possible new active compounds to treat AFB in honey bee colonies.
Assuntos
Anti-Infecciosos/farmacologia , Abelhas/microbiologia , Bacilos Gram-Positivos Formadores de Endosporo/efeitos dos fármacos , Própole/farmacologia , Animais , Brasil , Relação Dose-Resposta a Droga , Bacilos Gram-Positivos Formadores de Endosporo/isolamento & purificação , Técnicas In Vitro , Testes de Sensibilidade Microbiana , Esporos Bacterianos/efeitos dos fármacos , Esporos Bacterianos/isolamento & purificaçãoRESUMO
In this study, thermal inactivation parameters (D- and z-values) of Alicyclobacillus acidoterrestris spores in McIlvaine buffers at different pH, apple juice and apple nectar produced with and without ascorbic acid addition were determined. The effects of pH, temperature and ascorbic acid concentration on D-values of A. acidoterrestris spores were also investigated using response surface methodology. A second order polynomial equation was used to describe the relationship between pH, temperature, ascorbic acid concentration and the D-values of A. acidoterrestris spores. Temperature was the most important factor on D-values, and its effect was three times higher than those of pH. Although the statistically significant, heat resistance of A. acidoterrestris spores was not so influenced from the ascorbic acid within the concentration studied. D-values in apple juice and apple nectars were higher than those in buffers as heating medium at similar pH. The D-values ranged from 11.1 (90 degrees C) to 0.7 min (100 degrees C) in apple juice, 14.1 (90 degrees C) to 1.0 min (100 degrees C) in apple nectar produced with ascorbic acid addition, and 14.4 (90 degrees C) to 1.2 min (100 degrees C) in apple nectar produced without ascorbic acid addition. However, no significant difference in z-values was observed among spores in the juices and buffers at different pH, and it was between 8.2 and 9.2 degrees C. The results indicated that the spores of A. acidoterrestris may survive in fruit juices and nectars after pasteurization treatment commonly applied in the food industry.
Assuntos
Ácido Ascórbico/farmacologia , Bebidas/microbiologia , Manipulação de Alimentos/métodos , Bacilos Gram-Positivos Formadores de Endosporo/fisiologia , Temperatura Alta , Malus/microbiologia , Contagem de Colônia Microbiana , Qualidade de Produtos para o Consumidor , Relação Dose-Resposta a Droga , Microbiologia de Alimentos , Bacilos Gram-Positivos Formadores de Endosporo/efeitos dos fármacos , Bacilos Gram-Positivos Formadores de Endosporo/crescimento & desenvolvimento , Humanos , Concentração de Íons de Hidrogênio , Modelos Biológicos , Esporos Bacterianos/crescimento & desenvolvimento , TemperaturaRESUMO
The kdp region from the thermoacidophilic bacterium Alicyclobacillus acidocaldarius consists of two divergent operons: kdpZFABCN, which is tenfold induced at low K+ concentrations and encodes the K+-translocating P-type ATPase KdpZFABC as well as KdpN, a novel covalent homo-dimer of the cytoplasmic N-terminal part from sensor kinase KdpD; and secondly, the constitutively expressed kdpHE operon, encoding the remainder of KdpD and the response regulator KdpE.
Assuntos
Proteínas de Bactérias/genética , Genes Bacterianos , Bacilos Gram-Positivos Formadores de Endosporo/enzimologia , Bacilos Gram-Positivos Formadores de Endosporo/genética , Proteínas Quinases/genética , Adenosina Trifosfatases/genética , Adenosina Trifosfatases/metabolismo , Proteínas de Bactérias/metabolismo , Expressão Gênica/efeitos dos fármacos , Bacilos Gram-Positivos Formadores de Endosporo/efeitos dos fármacos , Dados de Sequência Molecular , Óperon , Potássio/farmacologia , Proteínas Quinases/metabolismoRESUMO
The adaptation time of Alicyclobacillus acidoterrestris CRA 7152 in orange juice was determined as a response to pH (3 to 5.8), temperature (20 to 54 degrees C), soluble solids concentration ((o)Brix; 11 to 19 (o)Brix), and nisin concentration (0 to 70 IU/ ml) effects. A four-factor central composite rotational design was used. Viable microorganisms were enumerated by plating on K medium (pH 3.7). Two primary models were used to represent growth and adaptation time. A second-order polynomial model was applied to analyze the effects of factors. Results showed that the Baranyi and Roberts model was better than the modified Gompertz model, considering the determination coefficient (R2) for experimental data description. Inhibition of bacteria can be obtained through several studied combinations for at least 47 days of storage. The shortest period of adaptation was observed between 37 to 45 degrees C, with pHs between 4 and 5, yet the longest periods of adaptation could be obtained around 20 degrees C with pHs close to 3.0. Statistical analysis of the quadratic model showed that the adaptation time increased as temperature or pH decreased, and as nisin concentration or soluble solids increased. The model showed that adaptation time has a minimum value for juice without nisin added, with 13.5% soluble solids, pH 5.0, and incubated at 43.8 degrees C. The statistical parameters that validated this model were an R2 of 0.816, a bias factor of 0.96, and an accuracy factor of 1.14. Manipulation of more than one factor, as well as the use of an antimicrobial agent, can be an alternative to preventing the development of A. acidoterrestris in orange juice, thus contributing to increased orange juice shelf life.
Assuntos
Adaptação Fisiológica , Antibacterianos/farmacologia , Bebidas/microbiologia , Conservação de Alimentos/métodos , Bacilos Gram-Positivos Formadores de Endosporo/fisiologia , Modelos Biológicos , Nisina/farmacologia , Citrus sinensis/microbiologia , Relação Dose-Resposta a Droga , Contaminação de Alimentos/análise , Manipulação de Alimentos/métodos , Microbiologia de Alimentos , Bacilos Gram-Positivos Formadores de Endosporo/efeitos dos fármacos , Bacilos Gram-Positivos Formadores de Endosporo/crescimento & desenvolvimento , Concentração de Íons de Hidrogênio , Cinética , Esporos Bacterianos/efeitos dos fármacos , Esporos Bacterianos/crescimento & desenvolvimento , Temperatura , Fatores de TempoRESUMO
The present study investigated the use of two-phase olive mill waste (TPOMW) as substrate for the production of exopolysaccharide (EPS) by the endospore-forming bacilli Paenibacillus jamilae. This microorganism was able to grow and produce EPS in aqueous extracts of TPOMW as a unique source of carbon. The effects of substrate concentration and the addition of inorganic nutrients were investigated. Maximal polymer yield in 100-ml batch-culture experiments (2 g l(-1)) was obtained in cultures prepared with an aqueous extract of 20% TPOMW (w/v). An inhibitory effect was observed on growth and EPS production when TPOMW concentration was increased. Nutrient supplementation (nitrate, phosphate, and other inorganic nutrients) did not increase yield. Finally, an adsorption experiment of Pb (II), Cd (II), Cu (II), Zn (II), Co (II), and Ni (II) by EPS is reported. Lead was preferentially complexed by the polymer, with a maximal uptake of 230 mg/g EPS.
Assuntos
Bacilos Gram-Positivos Formadores de Endosporo/metabolismo , Resíduos Industriais , Óleos de Plantas/metabolismo , Polissacarídeos Bacterianos/metabolismo , Biomassa , Meios de Cultura/química , Meios de Cultura/farmacologia , Fermentação , Bacilos Gram-Positivos Formadores de Endosporo/efeitos dos fármacos , Bacilos Gram-Positivos Formadores de Endosporo/crescimento & desenvolvimento , Microbiologia Industrial/métodos , Cinética , Metais Pesados/química , Metais Pesados/metabolismo , Azeite de Oliva , Polissacarídeos Bacterianos/químicaRESUMO
In this study, the effectiveness of an active polyvinylalcohol-based film against Alicyclobacillus acidoterrestris was assessed. The active film was fabricated by immobilizing an active compound on the surface of a polymeric matrix and then tested by putting the film in contact with a medium that had been inoculated with microbial cells. Microbiological tests showed that the film was antimicrobial against both a single strain and a culture cocktail of A. acidoterrestris, at 44 degrees C. By monitoring the viable cell concentration under three different packaging conditions, it was possible to demonstrate that the active film was equally effective against both the single strain and the culture cocktail and that it maintained this efficacy at various medium volumes. The same microbial tests were also conducted on viable spores of the investigated microorganism, inoculated both into a laboratory medium and apple juice. The results indicate that these viable spores were better inhibited than cells by the active film in both investigated media.
Assuntos
Anti-Infecciosos/farmacologia , Embalagem de Medicamentos/métodos , Conservação de Alimentos/métodos , Bacilos Gram-Positivos Formadores de Endosporo/efeitos dos fármacos , Muramidase/farmacologia , Álcool de Polivinil , Contagem de Colônia Microbiana , Qualidade de Produtos para o Consumidor , Meios de Cultura , Bacilos Gram-Positivos Formadores de Endosporo/crescimento & desenvolvimento , Humanos , Testes de Sensibilidade Microbiana , Esporos Bacterianos/efeitos dos fármacos , Esporos Bacterianos/crescimento & desenvolvimento , Fatores de TempoRESUMO
Alicyclobacillus acidoterrestris is a thermophilic spore-forming bacterium that spoils acidic juices. In the orchard, apples may be contaminated with spores which can potentially grow in the resulting juice and cause spoilage. This study was undertaken to evaluate the efficacy of gaseous chlorine dioxide against A. acidoterrestris spores on apple surfaces. A. acidoterrestris spores were inoculated onto apple surfaces and were placed at room temperature, in a tightly sealed chamber containing a chlorine dioxide generating sachet, low, medium, or high release, for 30 min, 1, 2, and 3 h. After exposure, surviving spores were enumerated on K agar. Chlorine dioxide treated apples were stored at 4 degrees C for 7 days to assess the effect on visual quality. Inoculated, untreated apples served as the visual quality control. After exposure to high and medium release sachets for 1 h, spores were reduced to an undetectable level, a 5 log10 reduction; however, visual quality was compromised. After 1, 2, and 3 h of exposure to low release sachets, spore reductions were 2.7, 3.7, and 4.5 log10, respectively. And, after 7 days of storage, there were no significant visual quality differences between the apples exposed to low release sachet for all treatment times when compared to the control. Gaseous chlorine dioxide can effectively reduce viable A. acidoterrestris spores on apple surfaces. Due to the efficacy and easy of use, chlorine dioxide gas sachets may be useful to maintain apple quality during storage and shipping.