RESUMO
Nondestructive assembly of a nanostructured enzyme platform is developed in combination of the specific biomolecular attraction and electrostatic coupling for highly efficient direct electron transfer (DET) of enzymes with unprecedented applicability and versatility. The biologically assembled conductive nanomesh enzyme platform enables DET-based flexible integrated biosensors and DET of eight different enzyme with various catalytic activities.
Assuntos
Condutividade Elétrica , Enzimas Imobilizadas/química , Nanoestruturas/química , Bacteriófago M13/enzimologia , Biocatálise , Transporte de Elétrons , Enzimas Imobilizadas/metabolismo , Glucose Oxidase/química , Glucose Oxidase/metabolismo , Ouro/química , Nanotubos de Carbono/química , Polietilenoimina/química , Eletricidade EstáticaRESUMO
Two family 11 endoxylanases (EC 3.2.1.8) were functionally displayed on the surface of bacteriophage M13. The genes encoding endo-1,4-xylanase I from Aspergillus niger (ExlA) and endo-1,4-xylanase A from Bacillus subtilis (XynA) were fused to the gene encoding the minor coat protein g3p in phagemid vector pHOS31. Phage rescue resulted in functional monovalent display of the enzymes as was demonstrated by three independent tests. Firstly, purified recombinant phage particles showed a clear hydrolytic activity in an activity assay based on insoluble, chromagenic arabinoxylan substrate. Secondly, specific binding of endoxylanase displaying phages to immobilized endoxylanase inhibitors was demonstrated by interaction ELISA. Finally, two rounds of selection and amplification in a biopanning procedure against immobilized endoxylanase inhibitor were performed. Phages displaying endoxylanases were strongly enriched from background phages displaying unrelated proteins. These results open perspectives to use phage display for analysing protein-protein interactions at the interface between endoxylanases and their inhibitors. In addition, this technology should enable engineering of endoxylanases into novel variants with altered binding properties towards endoxylanase inhibitors.
Assuntos
Aspergillus niger/enzimologia , Bacillus subtilis/enzimologia , Bacteriófago M13/enzimologia , Endo-1,4-beta-Xilanases/metabolismo , Proteínas de Membrana/metabolismo , Biblioteca de Peptídeos , Mapeamento de Interação de Proteínas/métodos , Aspergillus niger/genética , Bacillus subtilis/genética , Bacteriófago M13/genética , Clonagem Molecular , Endo-1,4-beta-Xilanases/genética , Regulação Enzimológica da Expressão Gênica/fisiologia , Regulação Viral da Expressão Gênica/fisiologia , Engenharia de Proteínas/métodos , Proteínas Recombinantes/metabolismoRESUMO
Phage display can be used as a protein engineering tool to select proteins with desirable binding properties from a library of randomly constructed mutants. Here, we describe the development of this method for the directed evolution of Bacillus subtilis lipase A, an enzyme that has marked properties for the preparation of pharmaceutically relevant chiral compounds. The lipase gene was cloned upstream of the phage g3p encoding sequence and downstream of a modified g3p signal sequence. Consequently, the enzyme was displayed at the surface of bacteriophage fd as a fusion to its minor coat protein g3p. The phage-bound lipase was correctly folded and fully enzymatically active as determined from the hydrolysis of p-nitrophenylcaprylate with K(m)-values of 0.38 and 0.33 mM for the phage displayed and soluble lipase, respectively. Both soluble lipase and lipase expressed on bacteriophages reacted covalently with a phosphonate suicide inhibitor. The phage does not hamper lipase binding, since both soluble and phage-bound lipase have a similar half-life of inactivation of approximately 5 min. Therefore, we conclude that the Bacillus lipase can be functionally expressed on bacteriophages as a fusion to the phage coat protein g3p. The specific interaction with the suicide inhibitor offers a fast and reproducible method for the future selection of mutant enzymes with an enantioselectivity towards new substrates.
Assuntos
Bacillus subtilis/enzimologia , Bacillus subtilis/genética , Bacteriófago M13/enzimologia , Bacteriófago M13/genética , Lipase/metabolismo , Fagos Bacilares/enzimologia , Fagos Bacilares/genética , Bacillus subtilis/metabolismo , Células Cultivadas , Clonagem Molecular , Inibidores Enzimáticos/química , Regulação Bacteriana da Expressão Gênica , Regulação Enzimológica da Expressão Gênica , Isomerismo , Lipase/química , Lipase/genética , Organofosfonatos/química , Biblioteca de Peptídeos , Ligação Proteica , Engenharia de Proteínas/métodos , Proteínas Recombinantes de Fusão/genética , Proteínas Recombinantes de Fusão/metabolismo , Especificidade por SubstratoRESUMO
Recently, we reported the successful use of the gVI-cDNA phage display technology to clone cDNAs coding for novel peroxisomal enzymes by affinity selection using immobilized antisera directed against peroxisomal subfractions (Fransen, M.; Van Veldhoven, P.P.; Subramani, S. Biochem. J., 1999, 340, 561-568). To identify other unknown peroxisomal enzymes, we further exploited this promising approach. Here we report the isolation and cloning of another novel human cDNA encoding a protein ending in the tripeptide AKL, a C-terminal peroxisomal targeting signal (PTS1). Primary structure analysis revealed that this molecule shared the highest sequence similarity to members of the 2,4-dienoyl-CoA reductase (DCR) family. However, functional analysis indicated that a recombinantly expressed version of the novel protein did not possess DCR activity with either 2-trans,4-trans-hexadienoyl-CoA or 2-trans,4-trans-decadienoyl-CoA as a substrate. The recombinant protein interacted with HsPex5p, the human PTS1-binding protein. Binding was competitively inhibited by a PTS1-containing peptide and was abolished when the last amino acid of the PTS1 signal was deleted. Transfection of mammalian cells with gene fusions between green fluorescent protein (GFP) and the human cDNA confirmed a peroxisomal localization and, therefore, the functionality of the PTS1. These results further demonstrate the suitability of the gVI-cDNA phage display technology for cDNA expression cloning using an antibody as a probe.
Assuntos
Bacteriófago M13/enzimologia , Ácidos Graxos Dessaturases/metabolismo , Oxirredutases atuantes sobre Doadores de Grupo CH-CH , Peroxissomos/enzimologia , Proteínas Virais de Fusão/metabolismo , Sequência de Aminoácidos , Animais , Clonagem Molecular , Escherichia coli/metabolismo , Técnicas In Vitro , Dados de Sequência Molecular , Coelhos , Saccharomyces cerevisiae/metabolismoRESUMO
We describe a convenient approach for concomitant functional characterization of peptide domains (monoclonal antibody epitopes, receptor-ligand, DNA-protein, and protein-protein interaction sites, etc) encoded by the sequential series of overlapping M13 subclones generated for nucleotide sequence determination of a new cDNA. We have employed this method to rapidly map the location and amino acid sequence of an epitope-containing domain within a polypeptide encoded by a newly isolated cDNA.