In vitro effect of Er: YAG laser irradiation in caries cavity preparation on biobehaviors of adjacent human dental pulp cells in the pulp chamber.

The erbium-doped yttrium aluminum garnet (Er: YAG) laser has been successfully applied in caries removal; however, little is known about proper parameters of Er: YAG laser on different conditions of caries removal, especially the influence of Er: YAG irradiation on human dental pulp cells (hDPCs). Here, we tested the effects of Er: YAG laser at different output energy levels (100, 200, 300, 400, and 500 mJ) on biobehaviors of hDPCs. To simulate clinical deep caries conditions, hDPCs were cultured on the pulpal side of 500-µm-thick dentin disks in an in vitro pulp chamber model. Temperature change, structural change, and ablation depth of dentin disk were also recorded. The findings suggested that the biological behaviors of hDPCs are strongly correlated with the energy output of the Er: YAG laser. Er: YAG laser irradiation at 100 mJ may be proper and safe for deep caries removal since it would not cause any adverse effect on hDPCs biobehaviors.

Er:YAG laser in selective caries removal and dentin treatment with chitosan: a randomized clinical trial in primary molars.

This study evaluated the effect of chitosan on dentin treatment after selective removal of caries lesions with Er:YAG laser in reducing Streptococcus mutans, as well as its effect on the performed restorations. The sample consisted of children (aged 7 to 9 years) with active carious lesions and dentin cavitation located on the occlusal surface of deciduous molars. Eighty teeth were randomly distributed into 4 groups according to the caries removal method: Er:YAG laser (250 mJ/4 Hz) or bur and dentin surface treatment: 2.5% chitosan solution or distilled water. The bacterial load of caries-affected dentin was quantified by counting CFU/mg (n = 10). The teeth were restored and evaluated at 7 days, 6 months, and 12 months using modified USPHS criteria (n = 20). Microbiological data was analyzed by Mann-Whitney and clinical analyses were done using Kruskal-Wallis and Dunn test (α = 0.05). The results showed that the Er:YAG laser significantly reduced the amount of Streptococcus mutans (p = 0.0068). After dentin treatment with chitosan, there was a significant reduction in the amount of Streptococcus mutans for both removal methods (p = 0.0424). For the retention and secondary caries criteria, no significant differences were observed along the evaluated time (p > 0.05). The laser-treated group was rated "bravo" for discoloration (p = 0.0089) and marginal adaptation (p = 0.0003) after 6 and 12 months compared to baseline. The Er:YAG laser reduced the amount of Streptococcus mutans and the chitosan showed an additional antibacterial effect. After 1 year, the Er:YAG laser-prepared teeth, regardless of the dentin treatment, showed greater discoloration and marginal adaptation of the restorations.

Caries removal with Er:YAG laser followed by dentin biomodification with carbodiimide and chitosan: Wettability and surface morphology analysis.

This study aimed to investigate dentin wettability and surface morphology after selective removal of carious lesion by erbium-doped yttrium aluminum garnet (Er:YAG) laser, followed by dentin biomodification with carbodiimide (EDC) and chitosan (CHI). Seventy-eight bovine dentin specimens were submitted to caries induction. Specimens were distributed according to methods of carious removal (n = 39): bur at low-speed (40,000 rpm) or Er:YAG laser (noncontact mode, 250 mJ/pulse and 4Hz). All specimens were etched with 35% phosphoric acid, and subdivided according to dentin biomodification (n = 13): Control (no biomodification), EDC or CHI. The contact angle (n = 10) between adhesive system (3M ESPE) and dentin surface was measured by a goniometer. Eighteen specimens (n = 3) were analyzed by scanning electron microscopy. Data were analyzed by two-way ANOVA and Tukey's test (α = .05). The method used to remove carious lesion did not influence the wettability of dentinal surface (p = .748). The angles produced on the remaining dentin after biomodification were influenced (p = .007). CHI promoted higher contact angles (p = .007) and EDC did not differ from the control group (p = .586). In the bur-treated group, most tubules were open, regardless of which biomodifier was used. Laser modified the organic matrix layer. CHI promoted partially closed tubules in some areas while EDC exposed dentinal tubules. Regardless of which method was used for selective removal of carious lesion, biomodification with EDC did not affect the dentin wettability, whereas CHI changed the wettability of remaining dentin. Both biomodifiers promoted a slight change on dentin morphology.

Effects of 10,600 nm Carbon Dioxide Laser on Remineralizing Caries: A Literature Review.

Objective: To study the effects of carbon dioxide (CO2) lasers (λ = 10,600 nm) on remineralizing dental caries. Methods: This study involved performing a systematic search of English articles archived in the PubMed, Scopus, and Web of Science databases. The keywords used to identify the relevant articles were ((CO2 laser) OR (carbon dioxide laser)) AND ((dental caries) OR (tooth remineralization)). Publications before 2019 were selected. The titles and abstracts of the initially identified articles were screened. Duplicate records, reviews, and irrelevant studies were removed. Full texts were retrieved for publications that studied the effects of CO2 lasers on remineralizing dental caries. Results: The search identified 543 potentially relevant publications. A total of 285 duplicate records were removed. Sixteen articles were included in this review. Four studies reported that CO2 lasers inhibited bacterial growth. The growth of cariogenic bacteria, mainly Streptococcus mutans, on an irradiated tooth surface was slower compared with nonirradiated ones. Four studies investigated the reduction of the demineralization of enamel with cariogenic challenge. They found that CO2 lasers reduced the carbonate content of mineralized tissues and increased the microhardness of enamel. Nine studies used CO2 lasers associated with topical fluorides in remineralizing dental caries. The results of the synergistic effect of laser irradiation and fluoride application with regard to the inhibition of caries progression varied among these studies, whereas laser irradiation could enhance fluoride uptake to demineralized mineral tissues. Conclusions: CO2 laser irradiation increased acid resistance and facilitated the fluoride uptake of caries-like lesions. In addition, it reduced the growth of cariogenic bacteria.

Artificial Caries Resistance in Enamel after Topical Fluoride Treatment and 445 nm Laser Irradiation.

OBJECTIVE: This in vitro study is aimed at investigating the caries preventive effectiveness of 445 nm diode laser in combination with topical fluoridation. MATERIALS AND METHODS: A total of 30 caries-free bovine teeth were used in this study. Eighteen teeth were covered with nail varnish except four windows on the labial surface. The windows were assigned to no treatment/control (C), laser (L) (0.3 W, 60 s, and 90 J/cm2), fluoride (F), and fluoride followed by laser (FL) treatment groups. Artificial caries lesions were created, and the teeth were sectioned and investigated under polarized light microscopy for quantitative measurement of the resulted lesion depth. Ten teeth were used for surface temperature measurement and two teeth for scanning electron microscopy (SEM). Extra twelve human molars were used for the intrapulpal temperature measurement. The absorbance of fluoride at 445 nm was measured. RESULTS: The means of lesion depth for the C, L, F, and FL groups were 123.48 (±21.93), 112.33 (±20.42), 99.58 (±30.68), and 89.03 (±30.38) µm, respectively. The pairwise differences of the L, F, and FL groups compared with the C group were significant (p < 0.05). The differences between groups were tested: FL versus L p=0.02, F versus L p=0.16, and FL versus F p=0.91, and the difference of the F versus FL was not significant (p=0.91). Temperature increment at the enamel surface and pulp roof were ∆T = 16.67 (±4.11) and 2.12 (±0.66)°C, respectively. The topical fluoride absorbance at 445 nm is five orders higher than that at 810 nm. SEM shows that after laser irradiation the enamel surface was intact and without thermal damage. CONCLUSIONS: The 445 nm laser irradiation may be useful for caries prevention, and its effectiveness is lower than those previously achieved using the argon ion laser.

White Spot Lesion Remineralisation Agents in Primary Teeth: A Systematic Review.

PURPOSE: To perform a systematic review of articles related to the clinical efficacy of topical fluoride varnishes/gels, casein phosphopeptide-amorphous calcium phosphate (CPP-ACP), and other remineralisation agents of white spot lesions (WSL) in primary teeth. MATERIALS AND METHODS: Electronic and manual searches were conducted through diverse electronic databases. The search was limited to randomised, clinical, controlled trials, and quasi-experimental papers in full-text version. Suitable individual studies were evaluated through a previously reported quality system, their data extracted and carefully analysed. RESULTS: The search identified 298 citations, and 95 were chosen and reviewed in full text. Nine relevant citations met the eligibility criteria for inclusion in the systematic review. Pair comparisons were made between fluoride varnishes, CPP-ACP, dental lasers, and control interventions. CONCLUSIONS: Clinicians are encouraged to discuss more high-quality studies in order to provide sufficient evidence and to confirm the clinical utility of remineralisation agents of WSL in primary teeth.

Comparative study between laser and conventional techniques for class V cavity preparation in gamma-irradiated teeth (in vitro study).

OBJECTIVE: The purpose of this study was to compare laser with conventional techniques in class V cavity preparation in gamma-irradiated teeth. METHODS: Forty extracted human teeth with no carious lesions were used for this study and were divided into two main groups: Group I (n = 20) was not subjected to gamma radiation (control) and Group II (n=20) was subjected to gamma radiation of 60 Gray. Standard class V preparation was performed in buccal and lingual sides of each tooth in both groups. Buccal surfaces were prepared by the Er,Cr:YSGG laser (Waterlase iPlus) 2780 nm, using the gold handpiece with MZ10 Tip in non-contact and the "H" mode, following parameters of cavity preparation - power 6 W, frequency 50 Hz, 90% water and 70% air, then shifting to surface treatment laser parameters - power 4.5 W, frequency 50 Hz, 80% water and 50% air. Lingual surfaces were prepared by the conventional high-speed turbine using round diamond bur. Teeth were then sectioned mesio-distally, resulting in 80 specimens: 40 of which were buccal laser-treated (20 control and 20 gamma-irradiated specimens) and 40 were lingual conventional high-speed bur specimens (20 control and 20 gamma-irradiated specimens). RESULTS: Microleakage analysis revealed higher scores in both gamma groups compared with control groups. Chi-square test revealed no significant difference between both control groups and gamma groups (p=1, 0.819, respectively). A significant difference was revealed between all 4 groups (p=0.00018). CONCLUSION: Both laser and conventional high-speed turbine bur show good bond strength in control (non-gamma) group, while microleakage is evident in gamma group, indicating that gamma radiation had a dramatic negative effect on the bond strength in both laser and bur-treated teeth.

Comparative study between laser and conventional techniques for class V cavity preparation in gamma-irradiated teeth (in vitro study)

Abstract Objective: The purpose of this study was to compare laser with conventional techniques in class V cavity preparation in gamma-irradiated teeth. Methods: Forty extracted human teeth with no carious lesions were used for this study and were divided into two main groups: Group I (n = 20) was not subjected to gamma radiation (control) and Group II (n=20) was subjected to gamma radiation of 60 Gray. Standard class V preparation was performed in buccal and lingual sides of each tooth in both groups. Buccal surfaces were prepared by the Er,Cr:YSGG laser (Waterlase iPlus) 2780 nm, using the gold handpiece with MZ10 Tip in non-contact and the "H" mode, following parameters of cavity preparation - power 6 W, frequency 50 Hz, 90% water and 70% air, then shifting to surface treatment laser parameters - power 4.5 W, frequency 50 Hz, 80% water and 50% air. Lingual surfaces were prepared by the conventional high-speed turbine using round diamond bur. Teeth were then sectioned mesio-distally, resulting in 80 specimens: 40 of which were buccal laser-treated (20 control and 20 gamma-irradiated specimens) and 40 were lingual conventional high-speed bur specimens (20 control and 20 gamma-irradiated specimens). Results: Microleakage analysis revealed higher scores in both gamma groups compared with control groups. Chi-square test revealed no significant difference between both control groups and gamma groups (p=1, 0.819, respectively). A significant difference was revealed between all 4 groups (p=0.00018). Conclusion: Both laser and conventional high-speed turbine bur show good bond strength in control (non-gamma) group, while microleakage is evident in gamma group, indicating that gamma radiation had a dramatic negative effect on the bond strength in both laser and bur-treated teeth.

Paediatric laser dentistry. Part 2: Hard tissue laser applications.

AIM: Erbium lasers can provide effective and minimally invasive caries removal in children. The bonding phase remains a critic step as well as the choice of material. Glass ionomers exhibits lower bonding properties in laser irradiated teeth compared to the conventional method or to composite and resin modified glass ionomer. Laser can also provide effective decontamination and coagulation effects in vital and non vital pulp therapy of primary teeth, improving and simplifying the cleaning and disinfecting steps.

Applications of Light Amplification by Stimulated Emission of Radiation (Lasers) for Restorative Dentistry.

Light amplification by stimulated emission of radiation (laser) has been used widely in a range of biomedical and dental applications in recent years. In the field of restorative dentistry, various kinds of lasers have been developed for diagnostic (e.g. caries detection) and operative applications (e.g. tooth ablation, cavity preparation, restorations, bleaching). The main benefits for laser applications are patient comfort, pain relief and better results for specific applications. Major concerns for using dental lasers frequently are high cost, need for specialized training and sensitivity of the technique, thereby compromising its usefulness particularly in developing countries. The main aim of this paper is to evaluate and summarize the applications of lasers in restorative dentistry, including a comparison of the applications of lasers for major restorative dental procedures and conventional clinical approaches. A remarkable increase in the use of lasers for dental application is expected in the near future.

FT-Raman Spectroscopy Study of the Remineralization of Microwave-Exposed Artificial Caries.

Dental caries is a microbially mediated disease that can result in significant tooth structure degradation. Although the preponderance of lesions is treated by surgical intervention, various strategies have been developed for its noninvasive management. Here, we use a novel approach for noninvasive treatment based on killing Streptococcus mutans with high-frequency microwave energy (ME). The rationale for this approach is based on modulating the pH of caries to a physiological state to enable spontaneous tooth remineralization from exogenous sources. In the present study, after demonstrating that ME kills >99% of S. mutans in planktonic cultures, 8 enamel slabs were harvested from a single tooth. Baseline mineral concentration at each of 12 points per slab was obtained using Fourier transform (FT)-Raman spectroscopy. Surface demineralization was subsequently promoted by subjecting all samples to an S. mutans acidic biofilm for 6 d. Half of the samples were then exposed to high-frequency ME, and the other half were used as controls. All samples were next subjected to a remineralization protocol consisting of two 45-min exposures per 24-h period in tryptic soy broth followed by immersion in a remineralizing solution for the remaining period. After 10 d, samples were removed and cleaned. FT-Raman spectra were again obtained at the same 12 points per sample, and the mineral concentration was determined. The effect of the remineralization protocol on the demineralized slabs was expressed as a percentage of mineral loss or gain relative to baseline. The mineral concentration of the microwave-exposed group collectively approached 100% of baseline values, while that of the control group was in the order of 40%. Differences between groups were significant (P = 0.001, Mann-Whitney U test). We concluded that killing of S. mutans by ME promotes effective remineralization of S. mutans-demineralized enamel compared with controls.

CO2 laser and topical fluoride therapy in the control of caries lesions on demineralized primary enamel.

This study evaluated the effect of CO2 laser irradiation and topical fluoride therapy in the control of caries progression on primary teeth enamel. 30 fragments (3 × 3 × 2 mm) from primary canines were submitted to an initial cariogenic challenge that consisted of immersion on demineralizing solution for 3 hours and remineralizing solution for 21 hours for 5 days. Fragments were randomly assigned into three groups (n = 10): L: CO2 laser (λ = 10.6 µm), APF: 1.23% acidulated phosphate fluoride, and C: no treatment (control). CO2 laser was applied with 0.5 W power and 0.44 J/cm(2) energy density. Fluoride application was performed with 0.1 g for 1 minute. Cariogenic challenge was conducted for 5 days following protocol previously described. Subsurface Knoop microhardness was measured at 30 µm from the edge. Obtained data were subjected to analysis the variance (ANOVA) and Duncan test with significance of 5%. It was found that the L group showed greater control of deciduous enamel demineralization and were similar to those of APF group, while being statistically different from C group (P ≤ 0.05) that showed the lowest microhardness values. It was concluded that CO2 laser can be an additional resource in caries control progression on primary teeth enamel.

Effect of super short pulse Er:YAG laser on human dentin--Scanning electron microscopy analysis.

This study evaluated the effect of different pulse widths in the morphological characteristics of human dentin irradiated with Er:YAG in cavity preparation protocols and dentin pretreatment. Dentin discs with 2 mm thickness were obtained from 18 human molars. The experimental groups were composed from two variables: (1) clinical protocol-cavity preparation (E = 200 mJ/20 Hz)-and pretreatment (E = 80 mJ/2 Hz); and (2) pulse duration-50, 300, and 600 µs. This formed six experimental groups (n = 3): G1 (E = 200 mJ/20 Hz/50 µs); G2 (E = 200 mJ/20 Hz/300 µs); G3 (E = 200 mJ/20 Hz/600 µs); G4 (E = 80 mJ/2 Hz/50 µs); G5 (E = 80 mJ/2 Hz/300 µs); G6 (E = 80 mJ/2 Hz/600 µs). The samples were irradiated with the Er:YAG laser by noncontact mode at a focal distance of 7 mm from the target point under continuous water spray (60% water and 40% air). After the irradiation, they were processed for scanning electron microscopy (SEM). Morphological analysis showed an irregular dentin surface, absence of smear layer with opening of the exposure of dentinal tubules and protruding peritubular dentin-without indications of changes for all protocols used. Regardless of the analyzed experimental group, the dentin surface showed a microretentive morphology characteristic of ablation. The G1 and G4 showed a rougher surface when compared to other groups. Finally, we concluded that the pulse width can influence the morphological characteristics of the irradiated dentin tested in different clinical indications. The larger surface irregularity caused by regulation with less pulse width (50 µs) seems more appropriate to get a microretentive pattern necessary for successful adhesives restoration procedures.

Low-level laser therapy as an alternative for pulpotomy in human primary teeth.

This study aimed to evaluate the effects of low-level laser therapy (LLLT) on pulpal response of primary teeth. Twenty mandibular primary molars were randomly divided into the following groups: group I Buckley's formocresol (diluted at 1:5), group II calcium hydroxide, group III LLLT + zinc oxide/eugenol, and group IV LLLT + calcium hydroxide. LLLT parameters were set at 660-nm wavelength, 10-mW power output, and 2.5 J/cm(2) energy density for 10 s in continuous mode (InGaAlP laser, Twin Laser®, MMOptics, Sao Carlos, Sao Paulo, Brazil). The teeth were extracted at the regular exfoliation period. The dentin-pulp complex was graded by an established histopathological score system. Statistical analysis was performed by Kruskal-Wallis and chi-square test. The histopathological assessment revealed statistically significant differences among groups (P < 0.05). The lowest degree of pulpal inflammation was present in LLLT + calcium hydroxide (P = 0.0296). Calcium hydroxide showed the highest rate of hard tissue barrier (P = 0.0033), odontoblastic layer (P = 0.0033), and dense collagen fibers (P = 0.0095). On the other hand, formocresol showed the highest incidence of internal resorption (P = 0.0142). Based on this study, low-level laser therapy preceding the use of calcium hydroxide exhibited satisfactory results on pulp tissue healing. However, further clinical studies on human teeth with long-term follow-up are needed to test the low-level laser therapy efficacy.

Effects of diode laser on direct pulp capping treatment : a pilot study.

At the present time, evidence-based, best practices have yet to be established for maintaining the vitality of teeth by managing caries-associated pulp exposure in permanent teeth. In terms of biomechanical and esthetic considerations, pulp capping has proven to be more effective than root-canal therapy. Given the low success rate of conventional methods, new techniques, such as laser-assisted repairs, should be developed. The purpose of this study was to compare the effectiveness of conventional and diode laser-assisted methods in direct pulp capping of carious teeth. Ten patients ranging in age from 12 to 40 were assigned randomly to experimental and control groups in this clinical trial in which the participants' teeth were treated with different techniques, i.e., conventional treatment and diode 808-nm, laser-assisted treatment. For each of these groups, five cases were chosen for treatment with the same method under rubber dam isolation. The data were analyzed by the runs test using SPSS software. The success rate was significantly different between conventional (60 %) and diode 808-nm, laser-assisted (100 %) groups after one year (P > 0.05). The laser-assisted procedure proved to be more effective than the conventional technique in enhancing the outcomes of pulp-capping therapy in carious exposures.

A randomized clinical trial of the effect of low-level laser therapy before composite placement on postoperative sensitivity in class V restorations.

This study aimed to investigate the efficacy of low-level laser irradiation when applied just before placement of resin composite on reducing postoperative sensitivity of class V lesions. In this randomized clinical trial, 31 patients with 62 class V cavities were included (two teeth in each participant). The teeth were randomly assigned into laser and placebo groups. After cavity preparation, the teeth in the experimental group were subjected to irradiation from a low-power red laser (630 nm, 28 mW, continuous wave, 60 s, 1.68 J), which was applied for 1 min on the axial wall of the cavity. In the control group, the same procedure was performed but with laser simulation. Then, a self-etch adhesive was applied and the cavities were restored with a microhybrid resin composite. Before treatment and on days 1, 14, and 30 after treatment, tooth sensitivity to a cold stimulus was recorded using a visual analogue scale. Data were analyzed by Friedman and Wilcoxon signed-rank tests (p < 0.05). Pain scores after restorative procedures were significantly lower in the laser group compared to the placebo application (p < 0.05). Although both groups experienced a significant improvement in pain and discomfort throughout the follow-up periods (p < 0.001), the changes in visual analogue scale (VAS) scores between baseline and each follow-up examination were significantly greater in the laser than the placebo group (p < 0.05). Low-level laser therapy (LLLT) before placement of resin composite could be suggested as a suitable approach to reduce postoperative sensitivity in class V restorations.

Costs and effectiveness of treatment alternatives for proximal caries lesions.

OBJECTIVES: Invasive therapy of proximal caries lesions initiates a cascade of re-treatment cycles with increasing loss of dental hard tissue. Non- and micro-invasive treatment aim at delaying this cascade and may thus reduce both the health and economic burden of such lesions. This study compared the costs and effectiveness of alternative treatments of proximal caries lesions. METHODS: A Markov-process model was used to simulate the events following the treatment of a proximal posterior lesion (E2/D1) in a 20-year-old patient in Germany. We compared three interventions (non-invasive; micro-invasive using resin infiltration; invasive using composite restoration). We calculated the risk of complications of initial and possible follow-up treatments and modelled time-dependent non-linear transition probabilities. Costs were calculated based on item-fee catalogues in Germany. Monte-Carlo-microsimulations were performed to compare cost-effectiveness of non- versus micro-invasive treatment and to analyse lifetime costs of all three treatments. RESULTS: Micro-invasive treatment was both more costly and more effective than non-invasive therapy, with ceiling-value-thresholds for willingness-to-pay between 16.73 € for E2 and 1.57 € for D1 lesions. Invasive treatment was the most costly strategy. Calculated costs and effectiveness were sensitive to lesion stage, patient's age, discounting rate and assumed initial treatment costs. CONCLUSIONS: Non- and micro-invasive treatments have lower long-term costs than invasive therapy of proximal lesions. Micro-invasive therapy had the highest cost-effectiveness for treating D1 lesions in young patients. Decision makers with a willingness-to-pay over 16.73 € and 1.57 € for E2 and D1 lesions, respectively, will find micro-invasive treatment more cost-effective than non-invasive therapy.

Influence of laser irradiation on pits and fissures: an in situ study.

OBJECTIVE: The aim of this in situ study was to analyze the influence of the Er:YAG, Nd:YAG, and CO(2) lasers on the enamel acid resistance of pits and fissures. BACKGROUND DATA: The laser tissue interaction has been studied as a method of preventing occlusal caries. METHODS: Thirteen volunteers wore palatal acrylic appliances containing human occlusal enamel blocks that were divided into four groups (G1, control; G2, Er:YAG; G3, Nd:YAG; G4, CO(2)). Each palatal acrylic appliance was used in the four studied groups and was used for 14 consecutive days. A sucrose solution was applied to the specimens six times per day. The specimens were then sectioned in half, and a microhardness test was applied. The other halves were analyzed using polarized light microscopy to measure the caries-like lesion areas, and a morphological analysis was conducted using a scanning electron microscope (SEM). RESULTS: For the statistical analysis of the data obtained from the microhardness test (Knoop hardness number. [KHN]) (α=5%), Fisher's exact test was performed, and the group means were as follows: G1, 247±71; G2, 258±70; G3, 272±73; and G4, 298±56. The results demonstrated that the control group was significantly different from G3 and G4, which presented higher microhardness values. The Wilcoxon signed-rank test was used to analyze the data obtained from the caries-lesion area measurements (mm(2)) (α=5%) (G1, 0.01±1.08; G2, 0.13±0.18; G3, 0.05±0.17; and G4, 0.09±0.22). The results no showed significant differences among the groups in this analysis. CONCLUSIONS: Based on the results from the present study, it may be concluded that the CO(2) and Nd:YAG lasers increased the enamel acid resistance in pits and fissures.

The effect of fissure sealants on the values of two different caries detection devices.

OBJECTIVE: The aim of this in vitro study was to evaluate the effects of clear and opaque fissure sealants on readings of laser fluorescence (LF) and light-emitting diode (LED) based caries detection devices. BACKGROUND DATA: When planning patient care, the practitioner needs to consider any changes in the status of the sealed surface for the long-term success of the sealant. As visual inspection is difficult to perform on sealed surfaces, adjunct diagnostic methods must be used to improve follow-up assessments and increase the accuracy of caries diagnosis. METHODS: Forty-six freshly extracted permanent human molars were selected and divided into two groups. Each group was treated with a different sealant (clear and opaque). The teeth were measured twice by two blinded observers using an LF-based and an LED-based device before and after sealing. The data were analyzed using Wilcoxon's matched-pairs signed-rank test and a paired t-test. Cohen's κ and the intraclass correlation coefficient were used to examine intra- and inter-examiner repeatability. RESULTS: The values of the LED device were significantly higher after the application of the opaque sealant, but there was no statistically significant difference after the application of the clear sealant (p=0.15). The LF-based device readings were also significantly lower after both the clear and the opaque sealant applications (p<0.001). CONCLUSIONS: The readings from the LF-based device were affected by both sealants. The readings from the LED-based device were affected by the opaque sealant but not by the clear sealant.

In vitro assessment of laser efficiency for caries prevention in pits and fissures.

This study aimed to assess the in vitro efficacy of the lasers Er:YAG, Nd:YAG, and CO(2) operating in the low energy mode for caries prevention in pits and fissures. Forty-five caries-free enamel occlusal sections were randomly divided into three groups: G1 - Er:YAG (80 mJ/2 Hz); G2 - Nd:YAG Laser (1 W and 10 Hz); and G3 - CO(2) Laser (0.4 W and 20 Hz). After surface treatment, the samples were submitted to challenge with acid consisting of a 10-day immersion in demineralizing (6 h) and remineralizing solution (18 h). Next, enamel demineralization was quantitatively evaluated by subsurface microhardness test and polarized-light microscopy (PLM, mm(2)) and qualitatively assessed by scanning electron microscopy. The Wilcoxon test was used for comparison of each group with its own control. ANOVA (α = 5%) was employed for comparison among groups, and Fisher's LSD multiple comparison test was applied, to check the difference in means. Concerning the microhardness analyses, statistical difference between control, and experimental areas was only detected for the CO(2) group. Experimental values were higher than the controls. As for PLM analyses, smaller demineralized areas were measured for G2 (Nd:YAG) and G3 (CO(2)) compared with the control areas. In conclusion, the present findings suggest that the CO(2) laser should be selected in order to increase the enamel resistance to acid in pits and fissures.