RESUMO
Porcine reproductive and respiratory syndrome virus (PRRSV) causes a highly contagious disease that threatens the global swine industry. Recent studies have focused on the damage that PRRSV causes to the reproductive system of male pigs, although pathological research is lacking. Therefore, we examined the pathogenic mechanisms in male piglets infected with PRRSV. Gross and histopathological changes indicated that PRRSV affected the entire reproductive system, as confirmed via immunohistochemical analysis. PRRSV infected Sertoli cells and spermatogonia. To test the new hypothesis that PRRSV infection in piglets impairs blood - testis barrier (BTB) development, we investigated the pathology of PRRSV damage in the BTB. PRRSV infection significantly decreased the quantity and proliferative capacity of Sertoli cells constituting the BTB. Zonula occludens-1 and ß-catenin were downregulated in cell - cell junctions. Transcriptome analysis revealed that several crucial genes and signalling pathways involved in the growth and development of Leydig cells, Sertoli cells, and tight junctions in the testes were downregulated. Apoptosis, necroptosis, inflammatory, and oxidative stress-related pathways were activated, whereas hormone secretion-related pathways were inhibited. Many Sertoli cells and spermatogonia underwent apoptosis during early differentiation. Infected piglets exhibited disrupted androgen secretion, leading to significantly reduced testosterone and anti-Müllerian hormone levels. A cytokine storm occurred, notably upregulating cytokines such as tumour necrosis factor-α and interleukin-6. Markers of oxidative-stress damage (i.e. H2O2, malondialdehyde, and glutathione) were upregulated, whereas antioxidant-enzyme activities (i.e. superoxide dismutase, total antioxidant capacity, and catalase) were downregulated. Our results demonstrated that PRRSV infected multiple organs in the male reproductive system, which impaired growth in the BTB.
Assuntos
Barreira Hematotesticular , Síndrome Respiratória e Reprodutiva Suína , Vírus da Síndrome Respiratória e Reprodutiva Suína , Células de Sertoli , Testículo , Animais , Masculino , Suínos , Vírus da Síndrome Respiratória e Reprodutiva Suína/patogenicidade , Vírus da Síndrome Respiratória e Reprodutiva Suína/fisiologia , Síndrome Respiratória e Reprodutiva Suína/virologia , Síndrome Respiratória e Reprodutiva Suína/patologia , Células de Sertoli/virologia , Células de Sertoli/metabolismo , Barreira Hematotesticular/virologia , Testículo/virologia , Testículo/patologia , Espermatogônias/virologia , Apoptose , Células Intersticiais do Testículo/virologia , Citocinas/metabolismo , Testosterona/sangue , Proteína da Zônula de Oclusão-1/metabolismo , Proteína da Zônula de Oclusão-1/genéticaRESUMO
IMPORTANCE: SARS-CoV-2 is a new virus responsible for the Covid-19 pandemic. Although SARS-CoV-2 primarily affects the lungs, other organs are infected. Alterations of testosteronemia and spermatozoa motility in infected men have raised questions about testicular infection, along with high level in the testis of ACE2, the main receptor used by SARS-CoV-2 to enter host cells. Using an organotypic culture of human testis, we found that SARS-CoV-2 replicated with slow kinetics in the testis. The virus first targeted testosterone-producing Leydig cells and then germ-cell nursing Sertoli cells. After a peak followed by the upregulation of antiviral effectors, viral replication in the testis decreased and did not induce any major damage to the tissue. Altogether, our data show that SARS-CoV-2 replicates in the human testis to a limited extent and suggest that testicular damages in infected patients are more likely to result from systemic infection and inflammation than from viral replication in the testis.
Assuntos
SARS-CoV-2 , Testículo , Replicação Viral , Humanos , Masculino , SARS-CoV-2/fisiologia , Testículo/virologia , Células Intersticiais do Testículo/virologia , Células de Sertoli/virologiaRESUMO
The pandemic caused by Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) has led to several hypotheses of functional alteration of different organs. The direct influence of this virus on the male urogenital organs is still to be evaluated. However some hypotheses can already be made, especially in the andrological field, for the biological similarity of the SARS-CoV and SARS-CoV2. As well as SARS-CoV, SARS CoV-2 uses the 'Angiotensin Converting Enzyme-2' (ACE2) as a receptor to enter human cells. It was found that ACE2, Angiotensin (1-7) and its MAS receptors are present, over in the lung, also in the testicles, in particular in Leydig and Sertoli cells. A first hypothesis is that the virus could enter the testicle and lead to alterations in testicular functionality. A second hypothesis is that the binding of the virus to the ACE2 receptor, could cause an excess of ACE2 and give rise to a typical inflammatory response. The inflammatory cells could interfere with the function of Leydig and Sertoli cells. Both hypotheses should be evaluated and confirmed, in order to possibly monitor fertility in patients COVID-19+.
Assuntos
Betacoronavirus , Infecções por Coronavirus/complicações , Infertilidade Masculina/virologia , Pneumonia Viral/complicações , Enzima de Conversão de Angiotensina 2 , Betacoronavirus/metabolismo , COVID-19 , Infecções por Coronavirus/fisiopatologia , Humanos , Inflamação/virologia , Células Intersticiais do Testículo/enzimologia , Células Intersticiais do Testículo/virologia , Masculino , Pandemias , Peptidil Dipeptidase A/metabolismo , Pneumonia Viral/fisiopatologia , SARS-CoV-2 , Células de Sertoli/enzimologia , Células de Sertoli/virologia , Testículo/enzimologia , Testículo/virologiaRESUMO
BACKGROUND: Recombinant adeno-associated viruses (AAVs) are emerging as favoured transgene delivery vectors for both research applications and gene therapy. In this context, a thorough investigation of the potential of various AAV serotypes to transduce specific cell types is valuable. Here, we rigorously tested the infectivity of a number of AAV serotypes in murine testis by direct testicular injection. RESULTS: We report the tropism of serotypes AAV2, 5, 8, 9 and AAVrh10 in mouse testis. We reveal unique infectivity of AAV2 and AAV9, which preferentially target intertubular testosterone-producing Leydig cells. Remarkably, AAV2 TM, a mutant for capsid designed to increase transduction, displayed a dramatic alteration in tropism; it infiltrated seminiferous tubules unlike wildtype AAV2 and transduced Sertoli cells. However, none of the AAVs tested infected spermatogonial cells. CONCLUSIONS: In spite of direct testicular injection, none of the tested AAVs appeared to infect sperm progenitors as assayed by reporter expression. This lends support to the current view that AAVs are safe gene-therapy vehicles. However, testing the presence of rAAV genomic DNA in germ cells is necessary to assess the risk of individual serotypes.
Assuntos
Dependovirus/fisiologia , Terapia Genética/instrumentação , Vetores Genéticos/fisiologia , Testículo/virologia , Animais , Proteínas do Capsídeo/genética , Proteínas do Capsídeo/metabolismo , Dependovirus/classificação , Dependovirus/genética , Terapia Genética/métodos , Vetores Genéticos/genética , Células Intersticiais do Testículo/virologia , Masculino , Camundongos , Sorogrupo , Tropismo ViralRESUMO
NS5A is a multifunctional non-structural protein of classical swine fever virus (CSFV) that plays an important role in viral replication, but how it exerts its functions is unknown. Here, we report the cleavage of NS5A of the vaccine C-strain, resulting in two truncated forms (b and c). Further experiments using calpain- and caspase-family-specific inhibitors, followed by a caspase-6-specific shRNAs and inhibitor, showed that the cleavage of C-strain NS5A to produce truncated form c is mediated by caspase-6, mapping to 272DTTD275, while the cleavage producing truncated form b is probably mediated by another unknown protease. shRNA-mediated downregulation of caspase-6 and blocking of enzyme activity in ST cells significantly impaired genome replication and virus production, indicating that NS5A cleavage is required for CSFV replication.
Assuntos
Calpaína/genética , Caspase 6/genética , Vírus da Febre Suína Clássica/efeitos dos fármacos , Células Intersticiais do Testículo/efeitos dos fármacos , RNA Polimerase Dependente de RNA/química , Proteínas não Estruturais Virais/química , Clorometilcetonas de Aminoácidos/farmacologia , Sequência de Aminoácidos , Animais , Calpaína/antagonistas & inibidores , Calpaína/metabolismo , Caspase 6/metabolismo , Inibidores de Caspase/farmacologia , Linhagem Celular , Vírus da Febre Suína Clássica/genética , Vírus da Febre Suína Clássica/metabolismo , Células Intersticiais do Testículo/virologia , Masculino , Plasmídeos/química , Plasmídeos/metabolismo , Inibidores de Proteases/farmacologia , Proteólise/efeitos dos fármacos , RNA Interferente Pequeno/genética , RNA Interferente Pequeno/metabolismo , RNA Polimerase Dependente de RNA/genética , RNA Polimerase Dependente de RNA/metabolismo , Suínos , Testículo/efeitos dos fármacos , Testículo/virologia , Proteínas não Estruturais Virais/genética , Proteínas não Estruturais Virais/metabolismo , Replicação Viral/efeitos dos fármacosRESUMO
Mumps virus (MuV) infection frequently causes orchitis and impairs male fertility. However, the mechanisms underlying the innate immune responses to MuV infection in the testis have yet to be investigated. This study showed that MuV induced innate immune responses in mouse Sertoli and Leydig cells through TLR2 and retinoic acid-inducible gene I (RIG-I) signaling, which result in the production of proinflammatory cytokines and chemokines, including TNF-α, IL-6, MCP-1, CXCL10, and type 1 interferons (IFN-α and IFN-ß). By contrast, MuV did not induce the cytokine production in male germ cells. In response to MuV infection, Sertoli cells produced higher levels of proinflammatory cytokines and chemokines but lower levels of type 1 IFNs than Leydig cells did. The MuV-induced cytokine production by Sertoli and Leydig cells was significantly reduced by the knockout of TLR2 or the knockdown of RIG-I signaling. The local injection of MuV into the testis triggered the testicular innate immune responses in vivo. Moreover, MuV infection suppressed testosterone synthesis by Leydig cells. This is the first study examining the innate immune responses to MuV infection in testicular cells. The results provide novel insights into the mechanisms underlying the MuV-induced innate immune responses in the testis.
Assuntos
Imunidade Inata , Células Intersticiais do Testículo/imunologia , Vírus da Caxumba/imunologia , Caxumba/imunologia , Caxumba/virologia , Células de Sertoli/imunologia , Proteínas Adaptadoras de Transdução de Sinal/metabolismo , Animais , Citocinas/genética , Citocinas/metabolismo , Modelos Animais de Doenças , Regulação da Expressão Gênica , Fator Regulador 3 de Interferon/metabolismo , Células Intersticiais do Testículo/metabolismo , Células Intersticiais do Testículo/virologia , Masculino , Proteínas de Membrana/metabolismo , Camundongos , Camundongos Knockout , Caxumba/genética , Caxumba/metabolismo , NF-kappa B/metabolismo , Proteínas do Tecido Nervoso/metabolismo , Receptores de Superfície Celular , Células de Sertoli/metabolismo , Células de Sertoli/virologia , Testosterona/biossíntese , Receptor 2 Toll-Like/genética , Receptor 2 Toll-Like/metabolismoRESUMO
BACKGROUND: The immuno-privileged status of the testis is essential to the maintenance of its functions, and innate immunity is likely to play a key role in limiting harmful viral infections, as demonstrated in the rat. In men mumps virus infects Leydig cells and has deleterious effects on testosterone production and spermatogenesis. The aim of this study was to test whether mumps virus infection of isolated human Leydig cells was associated with an inhibition of their innate antiviral defences. METHODS: Leydig cell production of mRNA and protein for interferons (IFNs) and of three antiviral proteins-2'5' oligoadenylate synthetase (2'5'OAS), double-stranded RNA-activated protein kinase (PKR) and MxA-was investigated, in the absence or presence of mumps virus or viral stimuli including poly I:C, a mimetic of RNA viruses replication product. RESULTS: Stimulated or not, human Leydig cells appeared unable to produce routinely detectable IFNs alpha, beta and gamma. Although the level of PKR remained unchanged after stimulation, the expression of 2'5'OAS and MxA was enhanced following either mumps virus or poly I:C exposure (P < 0.05 versus control). CONCLUSIONS: Overall, our results demonstrate that mumps virus replication in human Leydig cells is not associated with a specific inhibition of IFNs or 2'5'OAS, MxA and PKR production and that these cells display relatively weak endogenous antiviral abilities, as opposed to their rat counterparts.
Assuntos
Antivirais/farmacologia , Imunidade Inata/fisiologia , Indutores de Interferon/farmacologia , Células Intersticiais do Testículo/virologia , Vírus da Caxumba/imunologia , Poli I-C/farmacologia , 2',5'-Oligoadenilato Sintetase/metabolismo , Células Cultivadas , Proteínas de Ligação ao GTP/metabolismo , Humanos , Interferons/metabolismo , Células Intersticiais do Testículo/efeitos dos fármacos , Células Intersticiais do Testículo/imunologia , Masculino , Vírus da Caxumba/patogenicidade , Proteínas de Resistência a Myxovirus , Replicação Viral/imunologia , eIF-2 Quinase/metabolismoRESUMO
We report an in vitro analysis of the spatial pattern of production of three antiviral proteins (2'5'oligoadenylate synthetase, 2'5'AS; double-stranded RNA-activated protein kinase, PKR; and Mx protein, Mx) in the rat testis, in basal conditions and following stimulation with interferon (IFN) or Sendai virus. The two major constituents of interstitial tissue--Leydig cells and macrophages--constitutively produce 2'5' oligoadenylate synthetase (2'5'AS), PKR and Mx. Production of an isoform of 2'5'AS was induced following Leydig cells stimulation by the Sendai virus. The most immature germ cells, spermatogonia, were devoid of 2'5'AS whatever the type of stimulation, whereas IFN treatment induced Mx production and increased PKR production in this cell type. IFN stimulation strongly increased PKR production in all three cell types. This new set of data extends our previous investigations and demonstrates that the testis possesses an anti-viral defense system involving IFNs and IFN-induced anti-viral proteins.
Assuntos
2',5'-Oligoadenilato Sintetase/metabolismo , Proteínas de Ligação ao GTP/metabolismo , Células Intersticiais do Testículo/metabolismo , Espermatogônias/metabolismo , eIF-2 Quinase/metabolismo , Animais , Linhagem Celular , Células Cultivadas , Humanos , Interferon-alfa/farmacologia , Interferon gama/farmacologia , Células Intersticiais do Testículo/efeitos dos fármacos , Células Intersticiais do Testículo/enzimologia , Células Intersticiais do Testículo/virologia , Macrófagos/efeitos dos fármacos , Macrófagos/metabolismo , Macrófagos/virologia , Masculino , Proteínas de Resistência a Myxovirus , Ratos , Vírus Sendai/fisiologia , Espermatogônias/efeitos dos fármacos , Espermatogônias/enzimologia , Espermatogônias/virologiaRESUMO
OBJECTIVES: With the use of highly active antiretroviral therapy, the identification of HIV reservoirs within the body has become an important issue. However, the testis has been largely ignored despite representing a pharmacologic sanctuary which could act as a viral reservoir. DESIGN: Because alterations in testosterone production have frequently been reported in HIV-infected individuals, we investigated whether the testosterone-producing Leydig cells could become directly infected by HIV-1, HIV-2 or SIV. METHODS: Purified Leydig cells were infected with a panel of HIV-1, HIV-2 and SIV strains and examined for expression of HIV/SIV receptors. Additionally, the impact of CD4 transduction on Leydig cell infection was determined. RESULTS: Leydig cells were unable to support productive infection of the seven HIV-1 isolates tested. No CD4, CXCR4 or CCR5 expression was evident on the surface of Leydig cells and transduction with a CD4 expressing adenovirus did not induce HIV-1 infection. In contrast, some primary and laboratory adapted CD4-independent HIV-2 and SIV strains were able to enter and replicate productively in Leydig cells. CONCLUSIONS: Our results suggest that Leydig cells do not represent a target for HIV-1 infection within the testis. In contrast, Leydig cells support HIV-2 and SIV infection and thus represent a potential target for infection. Receptor use and significance of HIV-2/SIV infection of Leydig cells remain to be determined.
Assuntos
Infecções por HIV/virologia , HIV-2/patogenicidade , Células Intersticiais do Testículo/virologia , Síndrome de Imunodeficiência Adquirida dos Símios/virologia , Vírus da Imunodeficiência Símia/patogenicidade , Linfócitos T CD4-Positivos/imunologia , Células Cultivadas , Suscetibilidade a Doenças , Infecções por HIV/imunologia , HIV-1/patogenicidade , Humanos , Masculino , Receptores de HIV/metabolismo , Receptores Virais/metabolismo , Síndrome de Imunodeficiência Adquirida dos Símios/imunologiaRESUMO
Several viruses infect the testis, inducing inflammation, which may lead to infertility. In this study we investigated the production in rat and human testicular cells exposed to the Sendai virus of several chemokines that play a major role in inflammatory processes. Exposure of rat testicular macrophages and Sertoli, Leydig, and peritubular cells to the Sendai virus led to the production of mRNA and protein for monocyte chemotactic protein-1 (MCP-1), regulated on activation normal T cell expressed and secreted protein, growth-related oncogene-alpha, and interferon-gamma-inducible protein-10. In rat peritubular cells exposed to the Sendai virus, MCP-1 production was time and dose dependent. In contrast, rat germ cells did not produce these chemokines. Chemokine synthesis was detected in human Leydig cells exposed to the Sendai virus, but not in human total germ cells, suggesting that rats and humans display similar responses in terms of chemokine production. MCP-1, regulated on activation normal T cell expressed and secreted protein, growth-related oncogene-alpha, and interferon-gamma-inducible protein-10 have been reported to be chemoattractants for a large variety of leukocytes. The ability of the Sendai virus to induce chemokine production in somatic cells (mostly peritubular and Leydig cells) may therefore increase the recruitment of leukocytes to sites of infection.
Assuntos
Quimiocina CCL2/biossíntese , Quimiocina CCL5/biossíntese , Quimiocinas CXC/biossíntese , Infecções por Respirovirus/genética , Vírus Sendai , Testículo/metabolismo , Testículo/virologia , Animais , Northern Blotting , Quimiocina CCL2/genética , Quimiocina CCL5/genética , Quimiocina CXCL10 , DNA/biossíntese , DNA/genética , Ensaio de Imunoadsorção Enzimática , Células Germinativas/metabolismo , Células Germinativas/virologia , Humanos , Células Intersticiais do Testículo/metabolismo , Células Intersticiais do Testículo/virologia , Macrófagos/metabolismo , Macrófagos/virologia , Masculino , RNA Mensageiro/biossíntese , RNA Mensageiro/genética , Ratos , Ratos Sprague-Dawley , Células de Sertoli/metabolismo , Células de Sertoli/virologia , Espermatócitos/metabolismo , Espermatócitos/virologia , Espermatogônias/metabolismo , Espermatogônias/virologiaAssuntos
Proteínas de Ligação ao GTP , Interferons/fisiologia , Testículo/imunologia , Testículo/virologia , Animais , Células Cultivadas , Interferon-alfa/biossíntese , Interferon-alfa/farmacologia , Interferon beta/biossíntese , Interferon gama/biossíntese , Interferon gama/farmacologia , Interferons/metabolismo , Células Intersticiais do Testículo/imunologia , Células Intersticiais do Testículo/virologia , Masculino , Proteínas de Resistência a Myxovirus , Biossíntese de Proteínas , Proteínas/genética , Ratos , Respirovirus/imunologia , Túbulos Seminíferos/citologia , Células de Sertoli/imunologia , Células de Sertoli/virologia , eIF-2 Quinase/biossíntese , eIF-2 Quinase/genéticaRESUMO
The pathogenesis of infertility associated with acute viral orchitis was investigated in a rabbit model. Infection of postpubertal male European rabbits with an attenuated strain of myxoma virus caused systemic disease with viral replication to high titres in the testes. Infected rabbits developed an interstitial orchitis and epididymitis. This was associated with degeneration of the seminiferous epithelium, decreased serum testosterone concentrations and increased serum LH concentrations. Virus was localized within the interstitial cells. Clearance of infectious virus from the testis occurred between day 20 and day 30 after infection, although viral DNA could still be detected by polymerase chain reaction at 120 days. Viral clearance was associated with a return to normal serum testosterone and LH concentrations. Anti-sperm antibodies were present in the serum as early as 5 days after infection and declined during the recovery phase. Rabbits were infertile at 60 days but returned to normal fertility 60-90 days after infection.