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3.
Artigo em Inglês | MEDLINE | ID: mdl-31906336

RESUMO

China has been actively taking actions to control carbon emissions and promoting development of a carbon market. However, there are many disadvantages in a carbon market, owing to various designs and policies still under trial and implementation. Adopting the multi-agents technique, we constructed a framework about national carbon market to estimate the effect of a different design of policy made on the GDP (Gross Domestic Product) and environment. In particular, national and regional abatement policies were analyzed in our study. The results showed the carbon-trading mechanism can effectively reduce carbon emissions and make a negative impact on GDP. National abatement can neither be too high nor too low for reducing carbon emissions and maintaining economic stability. For different regions, the central region was impacted the most by a carbon trading mechanism, and the east region was the opposite. Moreover, the "sweeping approach" policy should be replaced by a regional "discriminating policy" because the abatement requirement to the western region was low and to the eastern region was relatively high, which is more beneficial to China's regional development.


Assuntos
Carbono , Modelos Teóricos , Carbono/economia , Carbono/provisão & distribuição , Dióxido de Carbono/análise , China , Mudança Climática , Simulação por Computador , Produto Interno Bruto , Licenciamento/estatística & dados numéricos
4.
J Biosci Bioeng ; 129(4): 428-434, 2020 Apr.
Artigo em Inglês | MEDLINE | ID: mdl-31732259

RESUMO

Xylose, the main component of xylan, is the second most abundant sugar in nature after glucose. Consequently, xylose represents an attractive feedstock for the production of value-added compounds such as biosurfactants (BSs), which are produced by various bacteria and yeasts. In this study, we screened and isolated yeast strains that synthesize BSs using xylose as the sole carbon source. We applied matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) to screen for BS-producing yeasts and isolated eight strains as the liamocin producers. Two of the eight strains, AS37 and SK25, were identified as Aureobasidium melanogenum, which is known as black yeasts, by based on 26S ribosomal RNA gene sequences. Both strains produced a wide variety of liamocin structures from not only xylose but also glucose and sucrose. According to the MALDI-TOF MS analysis, signals corresponding to sodium ion adducts of di-, tri-, tetra-, penta- and hexa-acylated C6-liamocins and di-, tri- and tetra-acylated C5-liamocins were detected. In addition, their mono-acetylated form was also detected. The dominant sugar component of liamocins produced by strains AS37 and SK25 is mannitol as estimated by HPLC analysis. This is the first report to describe the screening of liamocins-producing yeasts using xylose as the sole carbon source.


Assuntos
Ascomicetos/isolamento & purificação , Ascomicetos/metabolismo , Carbono/metabolismo , Manitol/análogos & derivados , Manitol/metabolismo , Xilose/metabolismo , Bactérias , Carbono/provisão & distribuição , Manitol/química , Técnicas Microbiológicas/métodos , Óleos/metabolismo , RNA Ribossômico , Espectrometria de Massas por Ionização e Dessorção a Laser Assistida por Matriz
5.
Artigo em Inglês | MEDLINE | ID: mdl-29466281

RESUMO

Reducing carbon emissions, including emission abatement outsourcing at the supply-chain level, is becoming a significant but challenging problem in practice. Confronting this challenge, we therefore break down the practice to focus on a low-carbon supply chain consisting of one supplier, one manufacturer and one third-party emission-reducing contractor. The contractor offers a carbon reduction service to the manufacturer. In view of the increasing proportion of Greenhouse Gases (GHG) emissions and absence of carbon reduction policies in developing countries, we adopt the prospect of consumers' low-carbon preferences to capture the demand sensitivity on carbon emission. By exploiting the Mean-Variance (MV) model, we develop a supply chain game model considering risk aversion. Comparing the supply chain performances of the cases under risk neutrality and risk aversion, we investigate the impact of the risk aversion of the supplier and the manufacturer on the low-carbon supply chain performances, respectively. We show that the risk aversion of chain members will not influence the relationship underlain by the profit-sharing contract between the manufacturer and contractor, whereas they may extend the supplier's concerning range. Although the manufacturer's risk aversion has a positive impact on the wholesale price, interestingly, the supplier's impact on the wholesale price is negative. Furthermore, we propose a contract to coordinate the risk-averse low-carbon supply chain by tuning the aversion levels of the supplier and the manufacturer, respectively. Through numerical study, we draw on managerial insights for industrial practitioners to adopt a low carbon strategy potentially by managing the risk attitudes along the supply chain channel.


Assuntos
Carbono/provisão & distribuição , Serviços Terceirizados/organização & administração , Comércio , Comportamento do Consumidor , Serviços Terceirizados/economia , Risco , Gestão de Riscos
6.
PLoS One ; 11(8): e0159900, 2016.
Artigo em Inglês | MEDLINE | ID: mdl-27487044

RESUMO

Small humic forest lakes often have high contributions of methane-derived carbon in their food webs but little is known about the temporal stability of this carbon pathway and how it responds to environmental changes on longer time scales. We reconstructed past variations in the contribution of methanogenic carbon in the pelagic food web of a small boreal lake in Finland by analyzing the stable carbon isotopic composition (δ13C values) of chitinous fossils of planktivorous invertebrates in sediments from the lake. The δ13C values of zooplankton remains show several marked shifts (approx. 10 ‰), consistent with changes in the proportional contribution of carbon from methane-oxidizing bacteria in zooplankton diets. The results indicate that the lake only recently (1950s) obtained its present state with a high contribution of methanogenic carbon to the pelagic food web. A comparison with historical and palaeobotanical evidence indicates that this most recent shift coincided with agricultural land-use changes and forestation of the lake catchment and implies that earlier shifts may also have been related to changes in forest and land use. Our study demonstrates the sensitivity of the carbon cycle in small forest lakes to external forcing and that the effects of past changes in local land use on lacustrine carbon cycling have to be taken into account when defining environmental and ecological reference conditions in boreal headwater lakes.


Assuntos
Agricultura , Carbono/provisão & distribuição , Cadeia Alimentar , Lagos , Animais , Carbono/metabolismo , Finlândia , Florestas , Humanos , Invertebrados/metabolismo , Zooplâncton/metabolismo
7.
Curr Opin Chem Biol ; 29: 26-31, 2015 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-26256682

RESUMO

In the sectors of biofuel and renewable chemicals the big feedstock demand asks, first, to expand the spectrum of carbon sources beyond primary biomass, second, to establish circular processing chains and, third, to prioritize product sectors exclusively depending on carbon: chemicals and heavy-duty fuels. Large-volume production lines will reduce greenhouse gas (GHG) emission significantly but also low-volume chemicals are indispensable in building 'low-carbon' industries. The foreseeable feedstock change initiates innovation, securing societal wealth in the industrialized world and creating employment in regions producing biomass. When raising the investments in rerouting to sustainable biofuel and chemicals today competitiveness with fossil-based fuel and chemicals is a strong issue. Many countries adopted comprehensive bioeconomy strategies to tackle this challenge. These public actions are mostly biased to biofuel but should give well-balanced attention to renewable chemicals as well.


Assuntos
Biocombustíveis/provisão & distribuição , Biomassa , Carbono/provisão & distribuição , Biocombustíveis/análise , Biocombustíveis/economia , Carbono/análise , Carbono/economia , Efeito Estufa
8.
ScientificWorldJournal ; 2015: 198385, 2015.
Artigo em Inglês | MEDLINE | ID: mdl-25821848

RESUMO

Flexibility and innovation at creating shapes, adapting processes, and modifying materials characterize composites materials, a "high-tech" industry. However, the absence of standard manufacturing processes and the selection of materials with defined properties hinder the configuration of the composites materials supply chain. An interesting alternative for a "high-tech" industry such as composite materials would be to review supply chain lessons and practices in "low-tech" industries such as food. The main motivation of this study is to identify lessons and practices that comprise innovations in the supply chain of a firm in a perceived "low-tech" industry that can be used to provide guidelines in the design of the supply chain of a "high-tech" industry, in this case composite materials. This work uses the case study/site visit with analogy methodology to collect data from a Spanish leading producer of fresh fruit juice which is sold in major European markets and makes use of a cold chain. The study highlights supply base management and visibility/traceability as two elements of the supply chain in a "low-tech" industry that can provide guidelines that can be used in the configuration of the supply chain of the composite materials industry.


Assuntos
Abastecimento de Alimentos , Indústrias , Transferência de Tecnologia , Carbono/provisão & distribuição , Fibra de Carbono , Resinas Compostas/provisão & distribuição , Indústria Alimentícia
9.
Acta amaz ; 44(1): 8679-86, 2014. tab, graf
Artigo em Português | LILACS, VETINDEX | ID: biblio-1455175

RESUMO

Wood density (WD) is an important variable for estimating tree carbon stocks in terrestrial ecosystems. WD has been little studied in savanna areas in the Brazilian Amazon. The aim of this study was to investigate variation in WD in eight tree species that occur in the open savannas of Roraima, the largest savanna area in the northern Brazilian Amazon. Variations were observed in WD in relation to species and diameter along the vertical dimension of 75 individuals sampled at six study sites. We used the direct method to obtain wood pieces from the stem and crown. The results indicated significant interspecific differences. Roupala montana Aubl. was the species with highest WD (0.674 g cm-3). Significant variation in WD was found between the stem and the woody parts of the crown, irrespective of species or sampling site. The wood density of branchs with diameter between 5 and 10 cm can be used as a predictor of individual WD for the whole tree. We conclude that the WD of the eight tree species investigated has interspecific variability and that there is a discrepancy between the WD of stem and the woody parts of the crown. Distinctions detected here provide an important tool for improving tree carbon estimates in Amazonian savannas.


Densidade da madeira (DM) é uma variável importante para estimativas de estoques de carbono arbóreo em ecossistemas terrestres. Este tema é pobremente investigado em áreas de savana da Amazônia brasileira. O objetivo deste estudo foi investigar a DM das oito principais espécies arbóreas que ocorrem na savana aberta de Roraima, a maior área de savana do norte do bioma Amazônia. Foram verificadas as variações na DM em função da espécie e dos diferentes diâmetros observados ao longo da dimensão vertical de 75 indivíduos amostrados em seis sítios de coleta. Foi utilizado o método direto para obtenção de peças de madeira do fuste e da copa. Os resultados indicaram discrepância significativa interespecífica, sendo Roupala montana Aubl. a espécie de maior DM média (0,674 g cm-3). Foi detectado que existe variação significativa da DM entre as peças do fuste e da copa, independente da espécie e do sítio de coleta. A densidade da madeira de peças da copa com diâmetro entre 5 e 10 cm pode ser utilizada como preditora da DM média do indivíduo arbóreo. Nós concluimos que a DM das oito espécies arbóreas investigadas possui variabilidade interespecífica, com discrepâncias entre a DM do fuste e das partes lenhosas da copa. As distinções aqui detectadas devem ser considerados como uma importante ferramenta para melhorar as estimativas de estoque de carbono em áreas de savanas na Amazônia.


Assuntos
Biomassa , Carbono/provisão & distribuição , Madeira/análise , Pradaria , Ecossistema Amazônico , Flora/classificação
10.
Bioprocess Biosyst Eng ; 34(8): 1027-38, 2011 Oct.
Artigo em Inglês | MEDLINE | ID: mdl-21647681

RESUMO

Fibrolytic enzyme production by Aspergillus japonicus C03 was optimized in a medium containing agro-industrial wastes, supplemented with peptone and yeast extract. A 2(3) full factorial composite and response surface methodology were used to design the experiments and analysis of results. Tropical forages were hydrolyzed by A. japonicus C03 enzymatic extract in different levels, and they were also tested as enzymatic substrate. Optimal production to xylanase was obtained with soybean bran added to crushed corncob (1:3), 0.01% peptone, and 0.2% yeast extract, initial pH 5.0, at 30 °C under static conditions for 5 days of incubation. Optimal endoglucanase production was obtained with wheat bran added to sugarcane bagasse (3:1), 0.01% peptone, and 0.2% yeast extract, initial pH 4.0, at 30 °C, for 6 days, under static conditions. Addition of nitrogen sources as ammonium salts either inhibited or did not influence xylanase production. This enzymatic extract had a good result on tropical forage hydrolyzes and showed better performance in the Brachiaria genera, due to their low cell wall lignin quantity. These results represent a step forward toward the use of low-cost agricultural residues for the production of valuable enzymes with potential application in animal feed, using fermentation conditions.


Assuntos
Ração Animal , Aspergillus/enzimologia , Carbono/metabolismo , Celulase/biossíntese , Endo-1,4-beta-Xilanases/biossíntese , Nitrogênio/metabolismo , Animais , Aspergillus/metabolismo , Brachiaria/química , Carbono/provisão & distribuição , Celulase/química , Cynodon/química , Endo-1,4-beta-Xilanases/química , Fermentação , Concentração de Íons de Hidrogênio , Hidrólise , Nitrogênio/provisão & distribuição , Panicum/química , Peptonas/metabolismo , Ruminantes , Temperatura
11.
13.
J Mol Biol ; 377(4): 1024-37, 2008 Apr 04.
Artigo em Inglês | MEDLINE | ID: mdl-18304578

RESUMO

In this article, we report on the genetic analysis of the Schizosaccharomyces pombe open reading frames SPCC1322.01 and SPAC637.11, respectively, which encode proteins that are similar to the exoribonuclease Dss1p and the RNA helicase Suv3p, respectively, forming the mitochondrial degradosome of Saccharomyces cerevisiae. While the helicase Suv3p is exchangeable between S. cerevisiae and S. pombe, the functions of Dss1p and the putative fission yeast RNase protein are specific for each species. Unlike S. cerevisiae mutants lacking a functional degradosome, the major defect of fission yeast knock-out strains is their inability to perform downstream processing of transcripts. In addition, the lack of pah1 results in instability of mitochondrial RNA ends. Overexpression of par1 and pah1 has no significant effect on the steady-state levels of mitochondrial RNAs. The Pet127p-stimulated RNA degradation activity is independent of Par1p/Pah1p in fission yeast mitochondria. The results presented herein indicate that both fission yeast proteins play only a minor role (if at all) in mitochondrial RNA degradation. We assume that the RNA-degrading function was taken over by other enzymes in fission yeast mitochondria, while the former degradosome proteins were recruited to new cellular pathways, for example, RNA processing in fission yeast (as discussed in this article) or mitochondrial DNA replication, apoptosis, or chromatin maintenance in eukaryotes, during evolution.


Assuntos
Mitocôndrias/genética , Processamento de Terminações 3' de RNA/fisiologia , Schizosaccharomyces/genética , Fatores de Poliadenilação e Clivagem de mRNA/fisiologia , Carbono/metabolismo , Carbono/provisão & distribuição , RNA Helicases DEAD-box/genética , RNA Helicases DEAD-box/fisiologia , Exorribonucleases , Fermentação/fisiologia , Proteínas Fúngicas/genética , Proteínas Fúngicas/fisiologia , Regulação Fúngica da Expressão Gênica , Biblioteca Gênica , Teste de Complementação Genética , Mitocôndrias/metabolismo , Complexos Multiproteicos/metabolismo , Complexos Multiproteicos/fisiologia , Fases de Leitura Aberta/genética , Fases de Leitura Aberta/fisiologia , Organismos Geneticamente Modificados , Fosfatidato Fosfatase/genética , Fosfatidato Fosfatase/fisiologia , Fosfoproteínas Fosfatases , RNA Fúngico/metabolismo , Proteínas de Saccharomyces cerevisiae/genética , Proteínas de Saccharomyces cerevisiae/fisiologia , Schizosaccharomyces/crescimento & desenvolvimento , Schizosaccharomyces/metabolismo , Proteínas de Schizosaccharomyces pombe/genética , Proteínas de Schizosaccharomyces pombe/fisiologia , Regulação para Cima , Fatores de Poliadenilação e Clivagem de mRNA/metabolismo
14.
J Biotechnol ; 125(3): 408-15, 2006 Sep 18.
Artigo em Inglês | MEDLINE | ID: mdl-16650498

RESUMO

l-Ribulose is an important chiral lead molecule used for the synthesis of, among others, l-ribose, a high-value rare sugar used in the preparation of antiviral drugs. These drugs--nucleoside-analogues--gain importance in the treatment of severe viral diseases, like those caused by the HIV or hepatitis virus. In this study, factors that may have an impact on l-ribulose production with Gluconobacter oxydans and on the stability of l-ribulose were investigated. A bioconversion-type process, using washed resting cells, was chosen to produce l-ribulose from ribitol. In this process, the cell production and bioconversion phase were separated. The former was first optimized and a maximum cell mass of 1.5 g CDWL(-1) could be produced. For the bioconversion phase, the aeration level of the system proved to be one of the most critical factors; a maximal production rate of 15.7 g L(-1)h(-1) or 5.9 g(g CDW)(-1)h(-1) of l-ribulose could be reached. Furthermore, resting cells were found capable of completely converting ribitol solutions of up to 300 g L(-1) within 30 h, although the kinetics indicated a rather low affinity of the dehydrogenase enzymes for the substrate.


Assuntos
Gluconobacter oxydans/enzimologia , Oxirredutases/metabolismo , Pentoses/biossíntese , Ribitol/metabolismo , Acetobacter/enzimologia , Acetobacter/crescimento & desenvolvimento , Biomassa , Carbono/provisão & distribuição , Contagem de Células , Estabilidade de Medicamentos , Gluconobacter oxydans/crescimento & desenvolvimento , Concentração de Íons de Hidrogênio , Modelos Biológicos , Oxirredutases/farmacocinética , Oxigênio/farmacologia , Pentoses/metabolismo , Pentoses/farmacocinética , Ribitol/farmacocinética , Desidrogenase do Álcool de Açúcar/metabolismo , Desidrogenase do Álcool de Açúcar/farmacocinética , Fatores de Tempo
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