RESUMO
Leukocytederived microparticles (LMPs) include neutrophil, lymphocyte and monocytederived MPs. LMPs act as proinflammatory mediators in autoimmune diseases, infectious diseases and vascular diseases. The present study examined the hypothesis that the percentage of LMPs was increased in patients with inflamed odontogenic keratocysts (OKCs), and investigated the biological effects of Jurkat cellderived MPs on the fibroblasts of OKCs in vitro. Cyst fluid MPs, obtained by centrifugation of samples from 20 patients with inflamed OKCs, 3 patients with uninflamed OKCs, 15 patients with radicular cysts (RCs) and 12 patients with inflamed dentigerous cysts (DCs), were analyzed by transmission electron microscopy, dynamic light scattering and immunofluorescence staining. The percentages and concentrations of cyst fluid LMPs were further determined by flow cytometry. The cytokine levels of apoptotic Jurkat cellderived MPs and Jurkat cell supernatants were compared by cytokine antibody arrays. Fibroblasts were isolated from 3 patients with OKC and cocultured with apoptotic Jurkat cellderived MPs with or without interleukin (IL)15Rα to detect the levels of matrix metallopeptidase 9 (MMP9) and receptor activator of nuclear factorκB ligand (RANKL) by reverse transcriptionquantitative polymerase chain reaction and enzymelinked immunosorbent assay. The supernatant from Jurkat MPstreated fibroblasts was collected to make conditioned medium in which the osteoclastogenesis of Raw264.7 cells was determined. Antibodies against human soluble (s)RANKL were added to the conditioned medium to investigate the inhibitory effects. Mean percentages of lymphocyte and neutrophilderived MPs were significantly higher in inflamed OKCs than in DCs. Significant elevations in IL15 were detected in apoptotic Jurkat cellderived MPs compared with that in Jurkat cell supernatant. Furthermore, higher levels of MMP9 and RANKL were detected in Jurkat cell MPtreated OKC fibroblasts, and this was partially blocked by IL15Rα. Increased osteoclastlike cell formation was observed in the Jurkat MPstreated fibroblast supernatant and Raw264.7 coculture groups. The antihuman sRANKL antibody in the Jurkat MPstreated fibroblast supernatant group decreased the osteoclastogenesis of the Raw264.7 cells. These results indicate that LMPs serve as novel communication tools that contribute toward the bone resorption of inflamed OKCs by inducing RANKL of OKC fibroblasts via IL15.
Assuntos
Micropartículas Derivadas de Células/fisiologia , Interleucina-15/metabolismo , Linfócitos/citologia , Cistos Odontogênicos/imunologia , Ligante RANK/metabolismo , Adolescente , Adulto , Idoso , Animais , Micropartículas Derivadas de Células/metabolismo , Células Cultivadas , Criança , Técnicas de Cocultura , Feminino , Humanos , Células Jurkat , Linfócitos/metabolismo , Masculino , Camundongos , Pessoa de Meia-Idade , Células RAW 264.7 , Adulto JovemRESUMO
OBJECTIVE: The present study was designed to analyze the immunolocalization of proteins involved in cytoskeleton remodeling, such as moesin and Rho-A, in benign odontogenic lesions that present with expansive growth and invasive clinical behavior. MATERIALS AND METHODS: Expressions of moesin and Rho-A in odontogenic epithelium were evaluated by immunohistochemical analysis in 45 odontogenic lesions using monoclonal antibodies. RESULTS: Our results demonstrated strong membranous and cytoplasmic expressions of moesin in the epithelial cells in 66.7% and 44.4% of the odontogenic lesions, respectively. Furthermore, Rho-A expression in odontogenic epithelium was strong in the membrane and cytoplasm of 51.1% and 62.2% of the odontogenic lesions, respectively. A statistically significant correlation was found between the membranous and cytoplasmic expressions of moesin (pâ¯=â¯0.000) and those of Rho-A (pâ¯=â¯0.048) in odontogenic epithelial cells, while no statistically significant correlation was found between moesin and Rho-A expressions (pâ¯>â¯0.05). CONCLUSIONS: The present study confirmed the strong expressions of moesin and Rho-A by odontogenic epithelial cells, suggesting their involvement in the development of benign odontogenic lesions. However, this study has failed to detect the connection between the moesin and Rho-A interaction in expansive growth and local invasiveness of these lesions.
Assuntos
Citoplasma/metabolismo , Citoesqueleto/metabolismo , Epitélio/metabolismo , Proteínas dos Microfilamentos/metabolismo , Cistos Odontogênicos/metabolismo , Tumores Odontogênicos/metabolismo , Proteína rhoA de Ligação ao GTP/metabolismo , Adolescente , Adulto , Idoso , Criança , Citoplasma/imunologia , Citoesqueleto/imunologia , Epitélio/imunologia , Feminino , Humanos , Imuno-Histoquímica , Masculino , Proteínas dos Microfilamentos/imunologia , Pessoa de Meia-Idade , Cistos Odontogênicos/imunologia , Tumores Odontogênicos/imunologia , Proteína rhoA de Ligação ao GTP/imunologiaRESUMO
AIM: To analyse the immunoreactivity of IL-1α, TNF-α and IL-10 in odontogenic cysts and tumours and to investigate possible associations with established biological behaviours of these different lesions. METHODOLOGY: Immunohistochemical expression of anti-IL-1α, anti-TNF-α and anti-IL-10 antibodies was assessed on epithelium and mesenchyme of 20 radicular cysts (RCs), 20 residual cysts (RECs), 20 dentigerous cysts (DCs), 18 solid ameloblastomas (SAs), 20 keratocystic odontogenic tumours (KCOTs) and 15 dental follicles (DFs). Comparative analysis of data was performed using the nonparametric Wilcoxon signed-rank test and Kruskal-Wallis's test. RESULTS: Significantly greater expression of IL-1α in the epithelium was noted in RC, KCOT and SA (P = 0.01), whilst IL-10 and TNF-α was in the epithelium of RC, DC and KCOT (P < 0.01). In the mesenchyme, significantly greater immunopositivity was observed for IL-1α, IL-10 and TNF-α in KCOT, DC and RC (P < 0.01). In epithelial and mesenchymal tissues, there were a significant number of cases of RC and DC with IL-1α < IL-10 ratio (P < 0.01), whilst SA and KCOT showed IL-1α > IL-10 (P < 0.01). There was a significantly greater percentage of DF, DC and KCOT with TNF-α > IL10 ratio (P < 0.01). CONCLUSION: These results suggest involvement of the proteins in the pathogenesis of odontogenic cysts and tumours, with emphasis on the highest immunoreactivity of osteolysis stimulating factors in tumours with aggressive biological behaviour, such as SA and KCOT.
Assuntos
Cistos Odontogênicos/imunologia , Tumores Odontogênicos/imunologia , Cisto Radicular/imunologia , Saco Dentário/imunologia , Saco Dentário/patologia , Epitélio/imunologia , Epitélio/patologia , Humanos , Técnicas Imunoenzimáticas , Mesoderma/imunologia , Mesoderma/patologia , Cistos Odontogênicos/patologia , Tumores Odontogênicos/patologia , Cisto Radicular/patologia , Raiz Dentária/imunologia , Raiz Dentária/patologia , Fator de Necrose Tumoral alfaRESUMO
BACKGROUND: To compare the metallothionein (MT) immunoexpression in non-syndromic and syndromic keratocystic odontogenic tumour (KOT), to correlate MT with cellular proliferation, and to evaluate the influence of inflammation in MT. STUDY DESIGN: Fourteen cases of KOT were submitted to immunohistochemistry for MT and Ki-67 analysis. The lesions were grouped according to their grade of inflammation, and statistical analysis was performed. RESULTS: MT was higher in non-syndromic KOT than in syndromic KOT (p<0.05). No statistical difference in Ki-67 could be identified; however, an inverse correlation was observed between MT and Ki-67 in both lesions. When analysing inflammation, non-syndromic KOT showed no differences in either MT or Ki-67. CONCLUSIONS: The MT immunophenotype of syndromic KOT was different from non-syndromic KOT. MT might not be involved in the proliferation control of both KOT. MT and Ki-67 immunoexpressions proved to be unaffected by inflammation in non-syndromic KOT.
Assuntos
Metalotioneína/imunologia , Cistos Odontogênicos/imunologia , Humanos , Imuno-Histoquímica , Antígeno Ki-67/biossíntese , Antígeno Ki-67/imunologia , Metalotioneína/biossíntese , Cistos Odontogênicos/metabolismo , Cistos Odontogênicos/patologia , SíndromeRESUMO
The study involved 67 patients with odontogenic cysts (OC) aged 18 to 45 years, who were divided into groups: Group 1 (n = 67) patients with OC aged 18 to 45 years, group 2--control group, consisted of 20 healthy persons of similar age. We studied the characteristics of immune status and immunoreactivity in patients with odontogenic cysts. Condition of cellular and humoral immunity was assessed by using the methods of direct rosette developing with erythrocytes coated with monoclonal antibodies to CD3+, CD4+, CD8+, CD22+, CD4/CD8 indicators of immunoregulatory index and phagocytic immunity. State of nonspecific resistance was studied by determining the phagocytic activity of neutrophils and their oxygen dependent metabolism in NBT test. The concentration of cytokines (IL-6 and IL-4) in serum was determined by ELISA. During the study we found that in patients with (OC) developed significant changes in the structure of the immune response at the cellular as well as at the humoral level that makes it necessary to develop new individualized preventive measures along with existing therapies OC.
Assuntos
Imunidade Celular , Imunidade Humoral , Subpopulações de Linfócitos/imunologia , Cistos Odontogênicos/imunologia , Adolescente , Adulto , Antígenos CD/genética , Antígenos CD/imunologia , Estudos de Casos e Controles , Feminino , Expressão Gênica , Humanos , Imunofenotipagem , Interleucina-4/sangue , Interleucina-4/imunologia , Interleucina-6/sangue , Interleucina-6/imunologia , Subpopulações de Linfócitos/patologia , Masculino , Pessoa de Meia-Idade , Neutrófilos/imunologia , Neutrófilos/patologia , Cistos Odontogênicos/sangue , Cistos Odontogênicos/genética , Cistos Odontogênicos/patologia , Fagocitose , Formação de RosetaRESUMO
BACKGROUND: The aggressive biological behavior of odontogenic keratocysts (OKCs), unlike that of other odontogenic cysts, has argued for its recent re-classification as a neoplasm, 'keratocystic odontogenic tumor'. Identification of mutations in the PTCH gene in some of the OKCs that were expected to produce truncated proteins, resulting in loss of control of the cell cycle, provided additional support for OKCs having a neoplastic nature. METHODS: We investigated the immunohistochemical expression of the sonic hedgehog (SHH) signaling pathway-related proteins, PTCH, smoothened (SMO) and GLI-1, and of the SHH-induced bcl-2 oncoprotein in a series of primary OKC (pOKC), recurrent OKC (rOKC) and nevoid basal cell carcinoma syndrome-associated OKCs (NBCCS-OKCs), and compared them to solid ameloblastomas (SAMs), unicystic ameloblastomas (UAMs), 'orthokeratinized' OKCs (oOKCs), dentigerous cysts (DCs) and radicular cysts (RCs). RESULTS: All studied lesions expressed the SHH pathway-related proteins in a similar pattern. The expression of bcl-2 in OKCs (pOKCs and NBCCS-OKCs) and SAMs was significantly higher than in oOKCs, DCs and RCs (P < 0.001). CONCLUSIONS: The present results of the immunoprofile of OKCs (that includes the expression of the SHH-related proteins and the SHH-induced bcl-2 oncoprotein) further support the notion of OKC having a neoplastic nature. As OKCs vary considerably in their biologic behavior, it is suggested that the quality and quantity of interactions between the SHH and other cell cycle regulatory pathways are likely to work synergistically to define the individual phenotype and corresponding biological behavior of this lesion.
Assuntos
Proteínas Hedgehog/metabolismo , Neoplasias Maxilomandibulares/metabolismo , Cistos Odontogênicos/metabolismo , Receptores de Superfície Celular/metabolismo , Receptores Acoplados a Proteínas G/metabolismo , Fatores de Transcrição/metabolismo , Ameloblastoma/imunologia , Ameloblastoma/metabolismo , Ameloblastoma/patologia , Análise de Variância , Síndrome do Nevo Basocelular/imunologia , Síndrome do Nevo Basocelular/metabolismo , Síndrome do Nevo Basocelular/patologia , Estudos de Casos e Controles , Regulação Neoplásica da Expressão Gênica/fisiologia , Humanos , Imuno-Histoquímica , Doenças Maxilomandibulares/imunologia , Doenças Maxilomandibulares/metabolismo , Doenças Maxilomandibulares/patologia , Neoplasias Maxilomandibulares/classificação , Neoplasias Maxilomandibulares/imunologia , Neoplasias Maxilomandibulares/patologia , Cistos Odontogênicos/imunologia , Cistos Odontogênicos/patologia , Receptores Patched , Receptor Patched-1 , Proteínas Proto-Oncogênicas c-bcl-2/genética , Proteínas Proto-Oncogênicas c-bcl-2/metabolismo , Receptores de Superfície Celular/genética , Receptores Acoplados a Proteínas G/genética , Valores de Referência , Sistemas do Segundo Mensageiro/fisiologia , Transdução de Sinais/fisiologia , Receptor Smoothened , Fatores de Transcrição/genética , Proteína GLI1 em Dedos de ZincoRESUMO
OBJECTIVE: the role of p53 expression in odontogenic lesions has not been fully determined, but has been associated with cell proliferation. The purpose of this study was to analyze p53 and proliferating cell nuclear antigen (PCNA) expression in 4 different odontogenic lesions. DESIGN: expression of p53 and PCNA was analyzed in radicular and dentigerous cysts, odontogenic keratocysts, and calcifying odontogenic cysts (Gorlin cysts) using monoclonal antibodies for detection of p53 and PCNA. RESULTS: PCNA expression was significantly greater in the basal layer of radicular cysts and in the suprabasal layer of odontogenic keratocysts; the percentage of p53 positive cells was significantly greater in the suprabasal layer of odontogenic keratocysts. CONCLUSIONS: The patterns of p53 and PCNA expression in dentigerous and radicular cysts were similar although the two lesions are of different origin. In odontogenic keratocysts and Gorlin cysts, results indicate a different pattern of tumor growth.
Assuntos
Cistos Odontogênicos/imunologia , Cistos Odontogênicos/patologia , Antígeno Nuclear de Célula em Proliferação/biossíntese , Proteína Supressora de Tumor p53/biossíntese , Humanos , Imuno-Histoquímica , Antígeno Nuclear de Célula em Proliferação/análise , Proteína Supressora de Tumor p53/análiseRESUMO
OBJECTIVE: The purpose of this study was to present 12 additional cases of glandular odontogenic cyst (GOC) in the Department of Oral Pathology, School of Stomatology, Wuhan University, People's Republic of China, and to investigate their immunohistochemical cytokeratins (CKs) expression in the epithelial components. METHODS: A total of 12 GOCs were reviewed clinically and radiographically, and immunohistologic CKs AE1, 7, 8/18, 10/13, 14, 16, 19 and 20 were performed by using a standard biotin-streptavidin immunoperoxidase technique on paraffin sections. RESULTS: The present series showed that eight occurred in males and four in females. The mean age was 37.6 years with a peak incidence occurring in the third decades (six of 12). Mandibles were more affected than maxillas (7:5), especially anterior mandible (four of seven). Radiographically, ratio multilocular to unilocular radiolucencies was 5:7 usually with well-defined borders. Histologically, cystic spaces were lined by non-keratinized stratified epithelia containing focal plaque-like or whirlpool-like thickenings; surface epithelial layer-containing eosinophilic cuboidal cells; mucous cells; and mucin pools of microcystic areas in the epithelium. Immunohistochemistry showed that epithelium of GOCs stained for CKs AE1, 7, 8/18, 10/13, 14 and 19 with slight changes in their patterns, and no reaction to CKs 16 and 20. CONCLUSIONS: Most clinical and histologic features in this study were analogous to those reported west population, although with slight difference between them. Histologically, the morphology of the epithelium strongly suggested an odontogenic origin, and CKs expression of GOC was similar to that of odontogenic epithelium, suggesting histochemically that GOC might be derived from odontogenic epithelium.
Assuntos
Queratinas/análise , Doenças Mandibulares/imunologia , Doenças Maxilares/imunologia , Cistos Odontogênicos/imunologia , Adulto , Distribuição por Idade , Epitélio/imunologia , Epitélio/patologia , Feminino , Humanos , Masculino , Doenças Mandibulares/patologia , Doenças Maxilares/patologia , Pessoa de Meia-Idade , Cistos Odontogênicos/patologia , Distribuição por SexoRESUMO
Intracystic fluid pressure is thought to be involved in odontogenic cyst growth. In this study, we investigated the effects of positive pressure on the expression of interleukin-1alpha (IL-1alpha), matrix metalloproteinases (MMPs), and prostaglandin E2 (PGE2) in odontogenic keratocysts to determine whether this pressure stimulates inflammatory cytokine production and signaling of osteoclastogenic events. Positive pressure enhanced the expression of IL-1alpha mRNA and protein in odontogenic keratocyst epithelial cells, and increased the secretion of MMP-1, MMP-2, MMP-3, and PGE2 in a co-culture of odontogenic keratocyst fibroblasts and the epithelial cells. The pressure-induced secretions were inhibited by an interleukin-1 receptor antagonist. Recombinant human interleukin-1alpha (rhIL-1alpha) increased the secretion of MMP-1, MMP-2, MMP-3, and PGE2 in the fibroblasts. Furthermore, in the fibroblasts, rhIL-1alpha enhanced the expression of macrophage colony-stimulating factor (M-CSF) mRNA, and rhIL-1alpha-induced PGE2 increased the expression of nuclear factor kappaB ligand (RANKL) mRNA. Thus, positive pressure may play a crucial role in odontogenic keratocyst growth via stimulating the expression of IL-1alpha in epithelial cells.
Assuntos
Células Epiteliais/metabolismo , Interleucina-1/metabolismo , Doenças Maxilomandibulares/metabolismo , Metaloproteinase 3 da Matriz/metabolismo , Metaloproteinases da Matriz/metabolismo , Cistos Odontogênicos/metabolismo , Proteínas de Transporte/genética , Proteínas de Transporte/metabolismo , Células Cultivadas , Dinoprostona/metabolismo , Células Epiteliais/citologia , Fibroblastos/citologia , Fibroblastos/metabolismo , Humanos , Doenças Maxilomandibulares/imunologia , Doenças Maxilomandibulares/patologia , Fator Estimulador de Colônias de Macrófagos/genética , Fator Estimulador de Colônias de Macrófagos/metabolismo , Metaloproteinase 1 da Matriz/metabolismo , Metaloproteinase 2 da Matriz/metabolismo , Glicoproteínas de Membrana/genética , Glicoproteínas de Membrana/metabolismo , Cistos Odontogênicos/imunologia , Cistos Odontogênicos/patologia , Pressão , Ligante RANK , RNA Mensageiro/análise , Receptor Ativador de Fator Nuclear kappa-B , Estresse Mecânico , Regulação para CimaRESUMO
O cisto da região globulomaxilar é encontrado exclusivamente na maxila, no interior do osso, na junção da porção globular do processo nasal mediano com o processo maxilar- a fissura globulomaxilar - geralmente entre o incisivo lateral superior e o canino. Possui a forma de pêra invertida, radiotransparente e circundado por um halo esclerótico. Os dentes adjacentes possuem vitalidade, a não ser que estejam infectados. Este relato de caso clínico refere-se a uma paciente melanoderma, 50 anos, que apareceu na clínica de CTBMF, encaminhada de uma cidade vizinha com uma tumefação dolorida na região esquerda da face, na altura da bochecha. Já no exame clínico, foi feita punção para alívio do edema e dor. A paciente foi submetida à cirurgia, posteriormente, para remoção do cisto e o exame anátomo-patológico confirmou os achados histológicos consistentes com cisto na região globulomaxilar, provavelmente, um cisto residual
Assuntos
Humanos , Feminino , Pessoa de Meia-Idade , Cirurgia Bucal , Cistos Odontogênicos/etiologia , Cistos Odontogênicos/imunologia , Cistos Odontogênicos/patologia , Cistos Odontogênicos , Fissuras Dentárias/psicologiaRESUMO
In this, the first of three articles on the aggressive nature of the odontogenic keratocyst (OKC), there is a review of clinical and histological observations which indicated that this was an aggressive lesion with a predilection for recurrence unlike the majority of other jaw cysts. This led to the tentative suggestion that the OKC might be a benign neoplasm. Subsequently there were early laboratory investigations that compared proliferation rates of the OKC epithelium with other jaw cysts, comparative enzyme histochemistry to assess aspects of its metabolism and markers that would enable accurate presurgical diagnosis of this cyst. Comparative studies were also pursued on the walls of the OKC and other jaw cysts to identify factors that might influence the capacity of the OKC to resorb the bone surrounding it. The clinical and laboratory studies reviewed in this section provided cogent presumptive evidence of the distinctively aggressive nature of the OKC that led numbers of investigators to pursue immunocytochemical and genetic studies on this cyst. Parts 2 and 3 of this series review this work.
Assuntos
Doenças Mandibulares/patologia , Cistos Odontogênicos/patologia , Biomarcadores/análise , Reabsorção Óssea , Citocinas/metabolismo , Humanos , Isoantígenos/análise , Queratinas/análise , Lactoferrina/análise , Doenças Mandibulares/imunologia , Doenças Mandibulares/metabolismo , Metaloproteinases da Matriz/metabolismo , Índice Mitótico , Cistos Odontogênicos/imunologia , Cistos Odontogênicos/metabolismo , Proteína Relacionada ao Hormônio Paratireóideo , Proteínas/metabolismoRESUMO
Objetivo: Se realizó un estudio descriptivo comparativo para valorar la expresión de la proteína p53 en los quistes dentígeros (QD) y queratoquistes odontogénicos (QQO), con sus variantes ortoqueratósica (QQO-O) y paraqueratósica (QQO-P), con el propósito de relacionar el grado de expresión de la proteína p53 con el potencial de agresividad de las patologías mencionadas. Método: La proteína p53 fue identificada, empleando el anticuerpo monoclonal M7001 junto con técnica de inmunoperoxidasa estándar. Se emplearon un total de 25 muestras de tejido, fijadas en formalina neutra y embebidas en parafina, correspondientes a 13 quistes dentígeros y 12 queratoquistes odontogénicos, previamente diagnosticados histológicamente. Estas fueron obtenidas de los archivos de Patología Oral de la Facultad de Odontología de la Pontificia Universidad Javeriana. La expresión de la proteína p53 fue medida observando el número de células con núcleos teñidos de color café en el epitelio quístico y que fueron identificadas con el miroscopio óptico de luz. Resultados: Grado de expresión nulo (72 por ciento) distribuidos así: QD 52 por ciento, QQO-O 16 por ciento y QQO-P 4 por ciento; grado de expresión leve (8 por ciento distribuidos así: QQO-O 4 por ciento y QQO-P 4 por ciento; grado de expresión moderado (20 por ciento) se presentó únicamente en los QQO-P. Ninguna patología presentó grado de expresión alto. La variable localización también fue estudiada relacionándola con el grado de expresión; no mostró significancia clínica. Conclusiones: La proteína p53 marcó más en los queratoquistes odontogénicos comparada con los quistes dentígeros, posiblemente por los cambios de queratinización que se presentan en su epitelio, siendo ésta una característica histológica importante en lesiones con potencial de transformación neoplásica o carcinomaS propiamente dichos
Assuntos
Cistos Maxilomandibulares , Proteína Supressora de Tumor p53 , Cisto Dentígero/imunologia , Cistos Odontogênicos/imunologia , Biópsia , Microscopia , Biologia Molecular , Distribuição de Qui-Quadrado , Imuno-Histoquímica/métodos , Transformação Celular NeoplásicaRESUMO
A novel alpha-chemokine, designated KS1, was identified from an EST database of a murine immature keratinocyte cDNA library. The EST has 94% similarity to a recently cloned human gene, BRAK, that has no demonstrated function. Northern analysis of mouse and human genes showed detectable mRNA in brain, intestine, muscle and kidney. Tumour panel blots showed that BRAK was down-regulated in cervical adenocarcinoma and uterine leiomyoma, but was up-regulated in breast invasive ductal carcinoma. KS1 bound specifically to B cells and macrophages, as well as two B cell lines, CESS and A20, and a monocyte line, THP-1. KS1 showed no binding to naive or activated T cells. In addition, KS1 stimulated the chemotaxis of CESS and THP-1 cells but not T cells. The s.c. injection of KS1 creates a mixed inflammatory response in Nude and C3H/HeJ mice. The above data indicates that KS1 and its human homologue represents a novel non-ELR alpha-chemokine that may have important roles in trafficking of B cells and monocytes. We propose the name B cell- and monocyte-activating chemokine (BMAC) for this molecule to reflect the described biological functions.
Assuntos
Linfócitos B/imunologia , Quimiocinas CXC/genética , Monócitos/imunologia , Sequência de Aminoácidos , Animais , Sequência de Bases , Northern Blotting , Linhagem Celular , Quimiocinas CXC/análise , Quimiocinas CXC/metabolismo , Quimiocinas CXC/farmacologia , Quimiotaxia/efeitos dos fármacos , Citometria de Fluxo , Biblioteca Gênica , Humanos , Queratinócitos/imunologia , Camundongos , Camundongos Endogâmicos C3H , Camundongos Nus , Dados de Sequência Molecular , Cistos Odontogênicos/imunologia , RNA Mensageiro/análise , Pele/efeitos dos fármacos , Pele/imunologiaRESUMO
Os cistos odontogênicos represetam entidades de interesse na clínica odontológica em funçäo de sua frequência e comportamento biológico variado. Levando em consideraçäo que a atividade proliferativa pode interferir no curso clínico dessas lesöes, foi avaliado nestetrabalho, a expressäo do PCNA e da proteína p53 em 11 ceratocistos odontogênico, 12 cistos dentígero, 12 cistos odontogênicos calcificantes e 11 cistos radiculares, através do método da streptavidina-biotina. Todos os casos analisados demonstraram positividade ao PCNA. Os ceratocistos odontogênicos e os cistos odontogênicos calcificantes expressaram maior reatividade com índices de 42,6 porcento e 41,13 porcento, respectivamente, enquanto cistos dentígeros e radiculares exibiram imunorreatividade em 32,45 porcento e 26,45 porcento, dos cistos estudados. Os índices de positividade do PCNA foram comparados entre os 4 grupos de cistos através da análise de variância e teste qui-quadrado, näo sendo verificada, no entanto, difernça estatisticamentesignificante. Por outro lado, somento 2 casos de cistos odontogênicos calcificantes e 1 cisto dentígero expressaram a proteína p53, os índices foram expressos em porcentuais. Frente a estes dados, concluímos que näo foi possível relacionar a expressäo da proteína p53 e do antígeno nuclear de proliferaçäo celular (PCNA) à atividade proliferativa do epitélio cístico e seu comportamento biológico (
Assuntos
Humanos , Antígeno Nuclear de Célula em Proliferação/efeitos adversos , Antígeno Nuclear de Célula em Proliferação/fisiologia , Antígeno Nuclear de Célula em Proliferação/imunologia , Cistos Odontogênicos/diagnóstico , Cistos Odontogênicos/imunologia , Cistos Odontogênicos/patologia , Proteína Supressora de Tumor p53RESUMO
Proliferating cell nuclear antigen (PCNA) is a nuclear protein synthesized in the late G1 and S phase of the cell cycle, and immunohistochemical detection of the protein represents a useful marker for the proliferating fraction of cells in tissue specimens. PCNA expression was studied in odontogenic keratocysts (n = 15) and ameloblastomas (n = 46) using an avidin-biotin-peroxidase complex method on routinely processed paraffin sections. The percentage of PCNA-positive cells determined by point counting was significantly lower in the ameloblastomas (mean 9.4%, standard deviation (SD) 11.0) than in odontogenic keratocysts (mean 29.9%, SD 24.0). In ameloblastomas, the mean percentage of PCNA-positive cells was lowest in the acanthomatous pattern and highest in plexiform pattern. The mean percentage of PCNA-positive cells in plexiform pattern was non-significantly higher than that in follicular pattern. The mean percentage of PCNA-positive cells in plexiform and follicular patterns was significantly higher than that in cystic and acanthomatous patterns. The frequency of PCNA-positive cells was significantly higher in the peripheral cells of follicular and plexiform patterns than in the central cells of both patterns (p < 0.01). Therefore, peripheral cells were regarded as reserve cell of central cells. The mean percentage of PCNA-positive cells in the epithelial lining of odontogenic keratocyst was not significantly different from those in the peripheral cells of follicular and plexiform patterns of ameloblastoma. In contrast, the odontogenic keratocyst exhibited a mean percentage of PCNA-positive cells which was statistically higher than that in other histological elements of ameloblastomas. The present study suggests that odontogenic keratocyst is regarded as benign odontogenic tumour.
Assuntos
Ameloblastoma/imunologia , Doenças Maxilomandibulares/imunologia , Neoplasias Maxilomandibulares/imunologia , Cistos Odontogênicos/imunologia , Antígeno Nuclear de Célula em Proliferação/metabolismo , Ameloblastoma/patologia , Biomarcadores , Divisão Celular , Humanos , Imuno-Histoquímica , Doenças Maxilomandibulares/patologia , Neoplasias Maxilomandibulares/patologia , Cistos Odontogênicos/patologiaRESUMO
A monospecific and high titer polyclonal antibody, designated as Fas D, raised against synthetic polypeptides selected from a part of the human Fas antigen (aa 104-114), was used to identify the Fas antigen in human oral epithelia in normal and pathological states. The human gingival proteins had been extracted and analysed by an ABC (avidin-biotin complex) immunoblotting technique. The antibody interacted with a single band in gingival proteins with an estimated molecular weight of 35,000, which is in good agreement with that calculated from amino acid sequences of the human Fas antigen. Using an indirect immunohistochemical method, the antibody localized on the stratum spinosum and the basal part of the stratum corneum of normal human gingiva. Specimens obtained from patients with odontogenic keratocysts, leukoplakia, lichen planus, and squamous cell carcinoma were also stained with the antibody. The pattern of the Fas antigen distribution in oral stratified squamous epithelia was, with some overlapping, characteristic for each disease.
Assuntos
Gengiva/imunologia , Doenças da Gengiva/imunologia , Receptor fas/análise , Apoptose , Carcinoma de Células Escamosas/imunologia , Estudos de Casos e Controles , Epitélio/imunologia , Técnica Indireta de Fluorescência para Anticorpo , Neoplasias Gengivais/imunologia , Humanos , Immunoblotting , Leucoplasia Oral/imunologia , Líquen Plano Bucal/imunologia , Mucosa Bucal/imunologia , Cistos Odontogênicos/imunologiaRESUMO
The aim of the present study was to investigate epithelial cell proliferation in the linings of odontogenic cysts, including three different subtypes of odontogenic keratocyst (OKC), namely simple (non-recurrent), recurrent and basal cell naevus syndrome (BCNS)-associated lesions. Ki67 immunoreactivity in OKC (simple, n = 10; recurrent, n = 8; syndrome, n = 9), dentigerous cysts (DC, n = 5), radicular cysts (RC, n = 5) and normal oral mucosa (n = 7) was studied using a biotin-streptavidin-peroxidase method on paraffin sections after microwave treatment. Ki67+ epithelial cells were counted manually and related to the length of basement membrane (BM) as determined by TV image analysis. Data were analysed by the Mann-Whitney U test. The number of Ki67+ cells in simple OKC linings (53.1 +/- 17.8 cells/mmBM) was similar to that in oral epithelium (42.5 +/- 12.7 cells/mmBM; P > 0.2). However, both contained significantly more Ki67+ cells than DC (3.9 +/- 1.3 cells/mmBM) and RC linings (6.7 +/- 4.8 cells/mmBM; P < 0.006). The epithelial distribution of Ki67+ cells differed between groups, with the percentage of positive cells in basal layers in OKC linings (7.0 +/- 2.1%) being significantly lower than that of oral epithelia (35.9 +/- 5.6%), DC (78.4 +/- 8.4%) and RC (80.6 +/- 7.7%) linings respectively (P < 0.003). Comparison of Ki67 expression within the OKC group revealed no significant difference between simple and recurrent lesions (44.0 +/- 13.8 cells/mmBM; P > 0.3). However, OKC associated with BCNS contained significantly higher numbers of Ki67+ cells (91.8 +/- 35.6 cells/mmBM; P < 0.01).(ABSTRACT TRUNCATED AT 250 WORDS)
Assuntos
Síndrome do Nevo Basocelular/patologia , Proteínas de Neoplasias/análise , Proteínas Nucleares/análise , Cistos Odontogênicos/patologia , Análise de Variância , Síndrome do Nevo Basocelular/imunologia , Biomarcadores , Proteínas de Ciclo Celular , Divisão Celular , Humanos , Técnicas Imunoenzimáticas , Queratinas , Antígeno Ki-67 , Micro-Ondas , Neoplasias Bucais/imunologia , Neoplasias Bucais/patologia , Cistos Odontogênicos/imunologia , Inclusão em Parafina , Antígeno Nuclear de Célula em Proliferação/análise , Recidiva , Fase S/fisiologia , Estatísticas não ParamétricasRESUMO
Com o objetivo de estabelecer as características imunocitoquímicas de folículos pericoronários, cistos dentígeros e queratocistos odontogênicos, foram selecionados dos arquivos do Laboratório de Anatomia Patológica do Departamento de Patologia da Faculdade de Odontologia de Bauru USP, dez casos de folículos pericoronários com epitélio reduzido do orgäo do esmalte, dez casos de folículos pericoronários com metaplasia escamosa, dez casos de cistos dentígeros e dez casos de queratocistos odontogênicos, submetidos a evidenciaçäo imunocitoquímica de um painel constituído dos seguintes marcadores: citoqueratina de alto peso molecular, citoqueratina de baixo peso molecular, laminina, fibronectina, colágeno IV, vimentina e proteína S100. A partir dos resultados obtidos pudemos concluir que o padräo imunocitoquímico das 4 condiçöes estudadas permite uma diferenciaçäo diagnóstica entre folículos pericoronários, cistos dentígeros e queratocistos odontogênicos, utilizando-se a marcaçäo da proteína S100. A diferenciaçäo pela marcaçäo com a proteína S100 pode ser complementada pela evidenciaçäo das células de Langerhans, que caracteristicamente estäo presentes nos revestimentos epiteliais dos cistos dentígeros
Assuntos
Humanos , Masculino , Feminino , Criança , Adolescente , Adulto , Pessoa de Meia-Idade , Cisto Dentígero/imunologia , Cisto Dentígero/patologia , Cisto Dentígero/química , Cisto Dentígero/ultraestrutura , Cistos Odontogênicos/imunologia , Cistos Odontogênicos/patologia , Cistos Odontogênicos/química , Cistos Odontogênicos/ultraestrutura , Saco Dentário/imunologia , Saco Dentário/patologia , Saco Dentário/química , Saco Dentário/ultraestrutura , Colágeno , Fibronectinas , Queratinas , Laminina , Patologia Bucal , VimentinaRESUMO
The expression of epidermal growth factor receptor (EGFR) was investigated in 67 cases of odontogenic cysts and 35 cases of odontogenic tumors using monoclonal antibody to EGFR (Biomarker, Israel) to determine the presence and significance of this transmembrane growth factor receptor. The cystic epithelial cells of odontogenic cystic lesions (keratocyst 60%; primordial cyst 75%; radicular cyst 35%; and follicular cyst 47.4%) were positive to EGFR staining. Cytochemical characterization of EGFR in those cystic epithelium was cell membrane positive type as in the normal epithelium. No expression of EGFR was found in the odontogenic tumors. This diversity of EGFR represents no binding activity of EGF, or loss of EGFR in the tumor cell upon EGFR mediated growth in odontogenic tumors was suggested a different tumor cell growth factor status or microenvironment in cell proliferation mechanism at the cellular level in cysts and tumors of odontogenic origin.
Assuntos
Receptores ErbB/análise , Neoplasias Maxilomandibulares/química , Cistos Odontogênicos/química , Tumores Odontogênicos/química , Ameloblastoma/química , Ameloblastoma/imunologia , Anticorpos Monoclonais , Cisto Folicular/química , Cisto Folicular/imunologia , Humanos , Técnicas Imunoenzimáticas , Neoplasias Maxilomandibulares/imunologia , Cistos Odontogênicos/imunologia , Tumores Odontogênicos/imunologia , Cisto Radicular/química , Cisto Radicular/imunologiaRESUMO
A resoluçäo näo cirúrgica dos cistos periodontais apicais ainda é questionável, principalmente porque sua comprovaçäo depende de resultados obtidos clínica e radiograficamente, näo fundamentados em observaçöes microscópicas. A partir de 348 casos registrados e organizados nos arquivos do Departamento de Patologia, da Faculdade de Odontologia de Bauru da Universidade de Säo Paulo, utilizando-se das técnicas de coloraçäo de rotina e de imuno-histoquímica, propusemo-nos: 1) a observar microscopicamente as alteraçöes morfológicas do revestimento epitelial, da parede conjuntiva e do lume dos cistos periodontais apicais de dentes tratados ou näo endodonticamente e dos cistos periodontais residuais, para estabelecer-se padröes morfológicos microscópicos próprios; 2) interrrelacionar os dados obtidos nos diversos grupos experimentais, a fim de implicar os aspectos microscópicos que possam sugerir uma regressäo näo cirúrgica dos cistos periodontais apicais de dentes tratados endodonticamente; 3) analisar as distribuiçöes das células B, das células T e das células de Langerhans nas estruturas dos cistos periodontais apicais de dentes tratados ou näo endodonticamente e dos cistos residuais, para estabelecer-se padröes próprios na distribuiçäo das células imunológicas, marcadas imuno-histoquimicamente, em cada grupo experimental já mencionados, e, correlacioná-los com os padröes microscópicos próprios, para aferir uma provável regressäo näo cirúrgica desta periapicopa...