Enhancing Sports Injury Risk Assessment in Soccer Through Machine Learning and Training Load Analysis.

Sports injuries pose significant challenges in athlete welfare and team dynamics, particularly in high-intensity sports like soccer. This study used machine learning algorithms to assess non-contact injury risk in professional male soccer players from physiological and mechanical load variables. Twenty-five professional male soccer players with a first-time, non-contact muscle injury were included in this study. Recordings of external load (speed, distance, and acceleration/deceleration data) and internal load (heart rate) were obtained during all training sessions and official matches over a 4-year period. Machine learning model training and evaluation features were calculated for each of nine different metrics for a 28-day period prior to the injury and an equal-length baseline epoch. The acute surge in the values of each workload metric was quantified by the deviation of maximum values from the average, while the variations of cumulative workload over the last four weeks preceding injury were also calculated. Seven features were selected by the model as prominent estimators of injury incidence. Three of the features concerned acute load deviations (number of sprints, training load score-incorporating heart rate and muscle load- and time of heart rate at the 90-100% of maximum). The four cumulative load features were (total distance, high speed and sprint running distance and training load score). The accuracy of the muscle injury risk assessment model was 0.78, with a sensitivity of 0.73 and specificity of 0.85. Our model achieved high performance in injury risk detection using a limited number of training load variables. The inclusion, for the first time, of heart rate related variables in an injury risk assessment model highlights the importance of physiological overload as a contributor to muscle injuries in soccer. By identifying the important parameters, coaches may prevent muscle injuries by controlling surges of training load during training and competition.

External Training Loads and Soft-Tissue Injury Occurrence During Congested Versus Noncongested Periods in Football.

OBJECTIVE: To analyze the influence of congested and noncongested fixture periods during 2 seasons in a professional male football team on soft-tissue injury incidence and external load. METHODS: Thirty-three professional football players from a Portuguese Liga I team participated in this study. Weekly external load and soft-tissue injury rate and burden of 2 consecutive seasons (2021-22 and 2022-23) were analyzed. RESULTS: Total soft-tissue injury rate and burden for the 2 seasons were 3.9 and 3.2 injuries per 1000 hours and 71.8 and 60.5 days per 1000 hours for congested and noncongested periods, respectively. No significant differences were observed between congested and noncongested periods. Total high-speed running, sprint distance, distance above 80% and 90% of maximal velocity, and meters accelerating and decelerating above 2 m/s2 were significantly higher for noncongested weeks. Match accelerations and decelerations above 3 m/s2 were higher during congested periods and training during noncongested periods. No differences between the 2 periods were observed for the total number of accelerations and decelerations above 3 m/s2. Overall, physical outputs per week were higher for training during noncongested weeks, whereas matches during congested periods registered higher external load. CONCLUSIONS: No effect of a congested schedule was observed on soft-tissue injury rates and injury burden. Higher match exposure during congested periods increased external load performed per week, and during noncongested periods, training load was superior to congested weeks.

Training injuries in elite men's senior and academy (Super League) rugby league; an analysis of 224,000 exposure-hours.

OBJECTIVES: Report two-years of training injury data in senior and academy professional rugby league. DESIGN: Prospective cohort study. METHODS: Match and training time-loss injuries and exposure data were recorded from two-seasons of the European Super League competition. Eleven/12 (2021) and 12/12 (2022) senior and 8/12 (2021) and 12/12 (2022) academy teams participated. Training injuries are described in detail and overall match injuries referred to for comparison only. RESULTS: 224,000 training exposure hours were recorded with 293 injuries at the senior (mean [95 % confidence interval]; 3 [2-3] per 1000 h) and 268 academy level (2 [2-3] per 1000 h), accounting for 31 % and 40 % of all injuries (i.e., matches and training). The severity of training injuries (senior: 35 [30-39], academy: 36 [30-42] days-lost) was similar to match injuries. Lower-limb injuries had the greatest injury incidence at both levels (senior: 1.85 [1.61-2.12], academy: 1.28 [1.08-1.51] per 1000 h). Head injuries at the academy level had greater severity (35 [25-45] vs. 18 [12-14] days-lost; p < 0.01) and burden (17 [16-18] vs. 4 [4-5] days-lost per 1000 h; p = 0.02) than senior level. At the senior level, the incidence of contact injuries was lower than non-contact injuries (risk ratio: 0.29 [0.09-0.88], p = 0.02). CONCLUSIONS: Training injuries accounted for about a third of injuries, with similar injury severity to match-play. Within training there is a higher rate of non-contact vs. contact injuries. Whilst current injury prevention interventions target matches, these data highlight the importance of collecting high quality training injury data to develop and evaluate injury prevention strategies in training.

The Acute: Chronic Workload Ratio and Injury Risk in Semiprofessional Football Players.

The purpose of this study was to analyze the association and predictive capacity between the acute:chronic workload ratio (ACWR) and non-contact injuries in a semiprofessional football team. Seventeen football or soccer players from a Spanish Third Division football team participated voluntarily in this study. A prospective longitudinal study was developed during the 2020/2021 season. Twenty-four weeks were analyzed from October to March, including a regenerative microcycle due to the absence of competition during Christmas. Rate of perceived exertion (RPE) and session-rate of perceived exertion (sRPE) were registered for every training and game session. Afterward, acute and chronic workloads were calculated, and ACWR was subsequently derived from them. Furthermore, non-contact injuries were registered during the period mentioned. The main findings were that there is a poor correlation between the ACWR and non-contact injuries (r=0.069 (p<0.05)), and the use of the ACWR by itself is insufficient to predict the occurrence of non-contact injuries in a semiprofessional football team. Consequently, the ACWR is not an useful predictive tool for injuries in semiprofessional football teams.

Injury incidence and prevalence in elite short-course triathletes: a 4-year prospective study.

OBJECTIVE: To characterise the prevalence, incidence rate (IR) and burden of injuries in elite short-course triathletes over a 4-year training and competition period. METHODS: Fifty elite Australian triathletes were prospectively monitored for injury during four consecutive seasons (2018-2021). Injuries requiring medical attention were prospectively recorded and further subcategorised according to time loss. The IR and burden (injury IR×mean injury severity) were calculated per 365 athlete days, with sex differences in IR compared using IR ratios (IRR) from negative binomial regression models. RESULTS: Two hundred and sixty-six injuries were reported in 46 (92.0%) athletes, of which 67.3% resulted in time loss. The injury IR was 1.87 injuries per 365 athlete days (95% CI 1.70 to 2.80), and comparable between sexes (IRR 0.82, 95% CI 0.64 to 1.04, p=0.109). Most injuries (70.7%) were training related. The most frequently injured body sites were the ankle (15.8%), foot (12.4%) and lower leg (12.0%). Bone stress injuries (BSIs) were the most burdensome injury type with 31.38 days of time loss per 365 days (95% CI 24.42 to 38.34). Twenty athletes (40.0%) reported at least one bone stress injury (BSI) (range 0-3). The rate of BSIs in female athletes was three times greater compared with male athletes (IRR 2.99, 95% CI 1.26 to 7.07, p=0.013). CONCLUSION: Two-thirds of injuries reported in elite short-course triathletes resulted in time loss, with the majority occurring during training activities. Foot, ankle and other lower leg injuries had the highest incidence, with BSIs carrying the highest injury burden. The considerably higher rate of BSI observed in female athletes warrants consideration for future prevention strategies in female triathletes.

The Association Between Training Load Indices and Injuries in Elite Soccer Players.

ABSTRACT: Tiernan, C, Comyns, T, Lyons, M, Nevill, AM, and Warrington, G. The association between training load indices and injuries in elite soccer players. J Strength Cond Res 36(11): 3143-3150, 2022-To investigate the association between contact injuries, noncontact injuries, and training load indices, across different lag periods in elite soccer players. Internal load (session rate of perceived exertion) was collected from 15 elite soccer players over 1 season (40-weeks). Acute (7 days), chronic (28 days), acute:chronic workload ratio (ACWR) (uncoupled), exponentially weighted moving averages (EWMA) ACWR, and 2-, 3-, and 4-week cumulative load were calculated on a rolling weekly basis. Multilevel logistic regression was used to analyze the associations between contact, noncontact injuries, and training load indices, across different lag periods (5 and 7 days). A player was at a significantly higher risk of a noncontact injury 5 days later, if week-to-week acute load changes increased (odds ratio [OR] = 1.97). An increase in EWMA ACWR was associated with an increased risk of both a contact (OR = 1.30) and noncontact injury (OR = 1.35), 5 days later. An increase in 2-week cumulative load (OR = 1.77) was associated with an increased risk of a contact injury 7 days later and 3-week cumulative load (OR = 1.55) 5 days later. These findings suggest that to reduce the potential risk of a noncontact injury, training load should be gradually increased, avoiding an increase in week-to-week acute load change (≥9%) or EWMA ACWR (>1.20). Findings indicated that EWMA ACWR may be a more sensitive measure for detecting a player at a higher risk of an injury than ACWR. Furthermore, a high 2- and 3-week cumulative load was associated with an increased risk of a contact injury, which may indicate accumulated fatigue. Practitioners must note that this study investigated associations with injury risk and not injury prediction.

Dance Exposure, Individual Characteristics, and Injury Risk over Five Seasons in a Professional Ballet Company.

PURPOSE: This study aimed to describe the relationships between dance exposure, dancer characteristics, and injury risk across five seasons in a professional ballet company. METHODS: Dance exposure time and clinician-reported time-loss and medical attention injury data were prospectively collected from 118 professional dancers of The Royal Ballet between 2015/2016 and 2019/2020. Cox proportional hazards and shared frailty models were fitted to overuse and traumatic injuries; individualized robust Z-scores for 7- and 28-d accumulated exposure, and week-to-week change in exposure, age, sex, company rank, and injury history were included as time-varying covariates. RESULTS: Across 381,710 h of exposure, 1332 medical attention and 427 time-loss injuries were observed. Positive relationships were observed between week-to-week change in exposure and overuse time-loss (+1 Z-score hazard ratio (HR), 1.27; 95% confidence interval (CI), 1.06-1.53) and medical attention injury risk (+1 Z-score HR, 1.17; 95% CI, 1.06-1.28). A negative relationship was observed between 7-d accumulated exposure and overuse medical attention injury risk (+1 Z-score HR, 0.74; 95% CI, 0.66-0.84). Overuse time-loss injury risk was greater in soloists compared with the corps de ballet (HR, 1.47; 95% CI, 1.01-2.15) and in dancers with a higher previous injury rate (+1 injury per 1000 h HR, 1.06; 95% CI, 1.02-1.10). Only age was associated with traumatic time-loss (+1-yr HR, 1.05; 95% CI, 1.01-1.09) or medical attention injury risk (+1-yr HR, 1.04; 95% CI, 1.01-1.07). CONCLUSIONS: Professional ballet companies should implement training principles such as periodization and progression, particularly in the case of senior-ranking dancers, older dancers, and dancers with high rates of previous injury. These findings provide a basis for future prospective investigations into specific causal injury pathways.

Clinical Practice Guidelines for Exertional Rhabdomyolysis: A Military Medicine Perspective.

ABSTRACT: Exertional rhabdomyolysis (ER) is an uncommon condition with a paucity of evidence-based guidance for diagnosis, management, and return to duty or play. Recently, a clinical practice guideline for diagnosis and management of ER in warfighters was updated by a team of military and civilian physicians and researchers using current scientific literature and decades of experience within the military population. The revision concentrated on challenging and controversial clinical questions with applicability to providers in the military and those in the greater sports medicine community. Specific topics addressed: 1) diagnostic criteria for ER; 2) clinical decision making for outpatient versus inpatient treatment; 3) optimal strategies for inpatient management; 4) discharge criteria; 5) identification and assessment of warfighters/athletes at risk for recurrent ER; 6) an appropriate rehabilitative plan; and finally, 7) key clinical questions warranting future research.

Epidemiology and pain in elementary school-aged players: a survey of Japanese badminton players participating in the national tournament.

Pain is common in athletes which should be well managed. To identify risk factors for shoulder pain, and the association between shoulder pain, lower back pain and knee pain among elementary school-aged badminton players, we conducted a cross-sectional study to collect data of the past year among 611 elementary school age (7-12 years old) badminton players belonging to the Japan Schoolchildren Badminton Federation using a questionnaire. Odds ratio (OR) and 95% confidence interval (CI) were estimated by multivariate logistic regression analysis. The overall incidence rate of shoulder injuries, lower back injuries and knee injuries was 0.38 injuries per 1000 h of badminton training. Players with training time per day > 2.5 h were 2.64 times (95% CI 1.03-6.78, p = 0.043) more likely to sustain shoulder pain than those with training time per day ≤ 2.5 h. A significant association was revealed between shoulder pain and knee pain as well as between lower back pain and knee pain as training hours per day > 2.5 h. Moreover, lower back pain was significantly associated with shoulder pain independent of training hours per day (≤ 2.5 h: p = 0.001; > 2.5 h: p < 0.001). These findings indicate that training time per day is risk factor, and shoulder pain, lower back pain and knee pain were associated with each other in elementary school-aged badminton players.

Carbohydrate restriction following strenuous glycogen-depleting exercise does not potentiate the acute molecular response associated with mitochondrial biogenesis in human skeletal muscle.

PURPOSE: Carbohydrate (CHO) restriction could be a potent metabolic regulator of endurance exercise-induced muscle adaptations. Here, we determined whether post-exercise CHO restriction following strenuous exercise combining continuous cycling exercise (CCE) and sprint interval exercise could affect the gene expression related to mitochondrial biogenesis and oxidative metabolism in human skeletal muscle. METHODS: In a randomized cross-over design, 8 recreationally active males performed two cycling exercise sessions separated by 4 weeks. Each session consisted of 60-min CCE and six 30-s all-out sprints, which was followed by ingestion of either a CHO or placebo beverage in the post-exercise recovery period. Muscle glycogen concentration and the mRNA levels of several genes related to mitochondrial biogenesis and oxidative metabolism were determined before, immediately after, and at 3 h after exercise. RESULTS: Compared to pre-exercise, strenuous cycling led to a severe muscle glycogen depletion (> 90%) and induced a large increase in PGC1A and PDK4 mRNA levels (~ 20-fold and ~ 10-fold, respectively) during the acute recovery period in both trials. The abundance of the other transcripts was not changed or was only moderately increased during this period. CHO restriction during the 3-h post-exercise period blunted muscle glycogen resynthesis but did not increase the mRNA levels of genes associated with muscle adaptation to endurance exercise, as compared with abundant post-exercise CHO consumption. CONCLUSION: CHO restriction after a glycogen-depleting and metabolically-demanding cycling session is not effective for increasing the acute mRNA levels of genes involved in mitochondrial biogenesis and oxidative metabolism in human skeletal muscle.

The relationship between training load and pain, injury and illness in competitive swimming: A systematic review.

BACKGROUND: Research suggests that the frequency of training, combined with the repetitive motion involved in high volume swimming can predispose swimmers to symptoms of over-training. The prevention of pain, injury and illness is of paramount importance in competitive swimming in order to maximise a swimmer's ability to train and perform consistently. A significant factor in the prevention of pain, injury or illness is the appropriate load monitoring and management practices within a training programme. OBJECTIVE: The purpose of this systematic review is to investigate the relationship between training load and pain, injury and illness in competitive swimmers. METHODS: The databases SPORTDiscus, CINAHL, Scopus, MEDLINE and Embase were searched in accordance with PRISMA guidelines. Studies were included if they reported on competitive swimmers and analysed the link between training load and either pain, injury or illness. The methodological quality and study bias were assessed using the Joanna Briggs Institute Critical Appraisal Checklist. RESULTS: The search retrieved 1,959 articles, 15 of which were included for review. The critical appraisal process indicated study quality was poor overall. Pain was the most explored condition (N = 12), with injury (N = 2) and illness (N = 1) making up the remaining articles. There was no evidence of an association between training load and pain, while there may be some evidence to suggest a relationship between training load and injury or illness. CONCLUSIONS: The relationship between training load and pain, injury or illness is unclear owing to a host of methodological constraints. The review highlighted that youth, masters and competitive swimmers of a lower ability (e.g. club versus international) may need particular consideration when planning training loads. Winter periods, higher intensity sessions and speed elements may also need to be programmed with care. Monitoring practices need to be developed in conjunction with consensus guidelines, with the inclusion of internal training loads being a priority. Future research should focus on longitudinal prospective studies, utilising the session Rating of Perceived Exertion (sRPE) monitoring method and investigating the applicability of Acute/Chronic Workload Ratio (ACWR) and exponentially weighted moving average (EWMA). Improved methods and study design will provide further clarity on the relationship between load and pain, injury, and illness.

Training and technique choices predict self-reported running injuries: An international study.

OBJECTIVES: The aim of this study was to describe the self-reported injury, training, and running technique choices of regular runners in four international regions. DESIGN AND SETTING: 756 participants began an expert derived self-report online survey in Ireland, USA, Hong Kong and Australia. PARTICIPANTS: 325 participants completed the survey (age = 38 ± 10 years; weight = 68.0 ± 13.1 kg; height = 1.70 ± 0.10 m). MAIN OUTCOME MEASURES: Descriptive statistics are reported examining injury incidence and location; shoe and orthosis choices; and training and technique practices. A backwards logistic regression was implemented to examine associations between injury and training choices. RESULTS: 68.3% reported having an injury in the last year. 81.45% of these injuries were believed to be running related. A large variation in training and footwear choices were observed for respondents. The regression (P ≤ 0.001) explained 20% of the variance in injury selection (Nagelkerke R2) and was able to identify 73% of cases accurately. Associated injury factors included competitive running, running on more than one surface, younger age, having a lower running age, and a higher proportion of running at an easy intensity. CONCLUSIONS: The high amount of variability in runner's choices highlights the lack of consistent information being presented to them and may be the reason for the high injury incidence.

Injury prevention knowledge, beliefs and strategies in elite female footballers at the FIFA Women's World Cup France 2019.

OBJECTIVES: This study assessed knowledge, beliefs and practices of elite female footballers regarding injury prevention. METHODS: A survey was sent to players participating in the FIFA Women's World Cup France 2019. Questions covered three injury prevention domains: (1) knowledge; (2) attitudes and beliefs; (3) prevention practices in domestic clubs. Additionally, ACL injury history was assessed. RESULTS: Out of 552 players, 196 women responded (35.5%). More than 80% of these considered injury risk to be moderate or high. Players listed knee, ankle, thigh, head and groin as the most important injuries in women's football. The most important risk factors identified were low muscle strength, followed by poor pitch quality, playing on artificial turf, too much training, reduced recovery and hard tackles. In these elite players, 15% did not have any permanent medical staff in their domestic clubs, yet more than 75% had received injury prevention advice and more than 80% performed injury prevention exercises in their clubs. Players identified the two most important implementation barriers as player motivation and coach attitude. Two-thirds of players used the FIFA 11+ programme in their clubs. CONCLUSIONS: This diverse group of elite players demonstrated good knowledge of risk level and injury types in women's football. Of the risk factors emphasised by players, there was only one intrinsic risk factor (strength), but several factors out of their control (pitch quality and type, training volume and hard tackles). Still players had positive attitudes and beliefs regarding injury prevention exercises and indicated a high level of implementation, despite a lack of medical support.

Factors associated with musculoskeletal injuries in an infantry commanders course.

OBJECTIVES: To evaluate the prevalence of injuries among young combat soldiers as assessed and reported by the military physicians, and to investigate whether parameters such as anthropometric measures, postural balance, proprioceptive ability, and chronic ankle instability are related to injuries during and following an infantry commanders course. METHODS: In this cross-sectional study, 165 soldiers were tested for anthropometric measurements, proprioceptive ability, and dynamic postural balance (DPB), as well as for their responses to an ankle stability questionnaire (CAIT), on three occasions: pre-course, middle-course, and end-course testing. All musculoskeletal injuries were assessed and recorded in the digital medical file of each participant by specialist military physicians before and during/following the course. RESULTS: Ninety-eight soldiers (59.4%) were injured before the course. Forty soldiers (24.2%) incurred an injury during/following the course (with 33 out of the 40 [82.5%] soldiers that were injured during/following the course having also been injured before the course). Sixty soldiers had no injury before/during/following the course. A survival curve showed that half of the soldiers who were injured during/following the course (20 soldiers) were recorded as injured in the first half of the course (during the first 46 days of the 92-day course). Logistic regression showed that the pre-course parameters that were significant among injured soldiers compared with the non-injured soldiers were: low CAIT results (OR = 2.736, 95% CI = 1.178-6.354), high BMI (OR = 1.234, 95% CI = 1.082-1.406) and reduced proprioceptive ability (OR =.858, 95% CI =.797-.924). CONCLUSION: With a high prevalence of soldiers injured during and following a commanders course, a somatosensory intervention program should be generalized into the practical daily preparation and training routines of the soldiers for improving somatosensory abilities, optimizing military physical readiness, and for preventing future musculoskeletal injuries.

Risk Factors for Lower-Extremity Injuries in Female Ballet Dancers: A Systematic Review.

BACKGROUND: Ballet dancers have a high prevalence of injuries to the lower extremity. Many studies have investigated the relationship between dance injury and risk factors. However, risk factors for lower-extremity injury comparing recreational- and elite-level ballet dancers are scarce. OBJECTIVE: To systematically review available original studies to assess risk factors for lower-extremity injury in female ballet dancers between recreational and elite ballet dancers. DATA SOURCES: Five online databases [Web of Science, PubMed, OVID (Medline), EBSCO, and ProQuest] were searched systematically. STUDY SELECTION: Included studies had an analytic study design published in the past 11 years and investigated an association between potential risk factors and lower-extremity injury in female ballet dancers. STUDY APPRAISAL: Assessed independently by 2 reviewers using the Downs and Black (DB) criteria and Oxford Centre of Evidence-Based Medicine. RESULTS: Seventeen studies were included. Alignment was a risk factor for lower-extremity injury in both recreational and elite ballet dancers. In elite ballet dancers, poor lumbopelvic movement control, inappropriate transversus abdominis contraction, decreased lower-extremity strength, and poor aerobic fitness were risk factors for lower-extremity injury. In recreational ballet dancers, hypermobility of the hip and ankle and longer training hours were risk factors for lower-extremity injury. Mean DB score was 15.94 (SD 1.57). The majority of studies were retrospective cohort studies or had poor follow-up, with 7 level 2b studies, 6 level 3b studies (cross-sectional), and 4 level 1b studies (prospective cohort with good follow-up). CONCLUSIONS: Alignment was identified as a common risk factor for recreational and elite ballet dancers. Other risk factors differed between recreational ballet dancers and elite ballet dancers. Future studies are warranted to use a prospective study design, identify dance level-specific risk factors, and implement evidence-based prevention strategies.

Changes in countermovement jump performance and subjective readiness-to-train scores following a simulated soccer match.

The study investigated whether countermovement jump (CMJ) metrics and subjective responses to a readiness-to-train questionnaire (RTT-Q) tracked simulated match-induced acute fatigue. This was a randomized cross-over repeated measures study. Participants were assigned into one of two groups; CONTROL or LIST. The LIST group performed the Loughborough Intermittent Shuttle Run (LIST), which was designed to simulate the demands of a soccer match. The CONTROL performed light physical activity at an intensity of <65% of maximal heart rate. Each group performed three CMJ's and completed an RTT-Q before (PRE), and again at 24 and 48 h after the LIST and/or CONTROL interventions. At 24 h there were significant differences in RTT-Q answers between the Pre and 24 h for the LIST group for questions; "Do you feel physically strong today?" and "Do you have muscle soreness today?" (p = 0.02 and 0.0008, respectively). The questions "Do you feel mentally strong today?" and "Do you have muscle soreness today?" (p = 0.02 and p = 0.0001 respectively) were the only questions that had a significant difference between Pre and 48 h for the LIST group. None of the CMJ metrics (LIST or CONTROL) changed significantly at any stage of the experiment. Although fatigue was detected by changes in the RTT-Q at 24 and 48 h after the LIST, none of the CMJ metrics changed. These findings suggest that subjective measures are more sensitive to low-level fatigue than objective measures, thus effective monitoring should include both.

Changes in supramaximal M-wave amplitude at different regions of biceps brachii following eccentric exercise of the elbow flexors.

PURPOSE: Previous evidence from surface electromyograms (EMGs) suggests that exercise-induced muscle damage (EIMD) may manifest unevenly within the muscle. Here we investigated whether these regional changes were indeed associated with EIMD or if they were attributed to spurious factors often affecting EMGs. METHODS: Ten healthy male subjects performed 3 × 10 eccentric elbow flexions. Maximal voluntary contraction (MVC), muscle soreness and ultrasound images from biceps brachii distal and proximal regions were measured immediately before (baseline) and during each of the following 4 days after the exercise. Moreover, 64 monopolar surface EMGs were detected while 10 supramaximal pulses were applied to the musculocutaneous nerve. The innervation zone (IZ), the number of electrodes detecting largest M-waves and their centroid longitudinal coordinates were assessed to characterize the spatial distribution of the M-waves amplitude. RESULTS: The MVC torque decreased (~ 25%; P < 0.001) while the perceived muscle soreness scale increased (~ 4 cm; 0 cm for no soreness and 10 cm for highest imaginable soreness; P < 0.005) across days. The echo intensity of the ultrasound images increased at 48 h (71%), 72 h (95%) and 96 h (112%) for both muscle regions (P < 0.005), while no differences between regions were observed (P = 0.136). The IZ location did not change (P = 0.283). The number of channels detecting the greatest M-waves significantly decreased (up to 10.7%; P < 0.027) and the centroid longitudinal coordinate shifted distally at 24, 48 and 72 h after EIMD (P < 0.041). CONCLUSION: EIMD consistently changed supramaximal M-waves that were detected mainly proximally from the biceps brachii, suggesting that EIMD takes place locally within the biceps brachii.