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1.
Rev. cuba. hematol. inmunol. hemoter ; 37(2): e1297, 2021. graf
Artigo em Espanhol | LILACS, CUMED | ID: biblio-1289428

RESUMO

Introducción: La enfermedad granulomatosa crónica es una inmunodeficiencia primaria congénita del sistema inmune innato, originada por defectos en el complejo enzimático nicotinamida adenina dinucleótido fosfato oxidasa presente en células fagocíticas. Estos defectos funcionales causan incapacidad para producir especies reactivas del oxígeno en los fagocitos, que afectan la eliminación de algunos microorganismos patógenos dentro del fagolisosoma. El diagnóstico de esta enfermedad se realiza actualmente mediante la prueba de 1,2,3-dihidrorodamina asistida por citometría de flujo multiparamétrica, o la tinción de fagocitos con nitroazul de tetrazolio asistida por microscopio óptico. Objetivos: Describir los aspectos fisiopatológicos y moleculares de la enfermedad granulomatosa crónica; y discutir aspectos relacionados con las pruebas de diagnóstico antes mencionadas. Métodos: Se realizó una investigación bibliográfica-documental a partir de artículos científicos publicados desde 1933 hasta 2018, para ello fueron consultadas las bases de datos SciELO, PubMed y Springer. Desarrollo: Se exponen las características fisiopatológicas de la enfermedad granulomatosa crónica, así como la relación entre las mutaciones genéticas más abundantes en la población afectada y la gravedad de las manifestaciones clínicas que presentan los pacientes. Además, se analizan críticamente los beneficios y las deficiencias de dos técnicas que se utilizan actualmente para diagnosticar la enfermedad. Conclusiones: La enfermedad granulomatosa crónica puede generar consecuencias inmunológicas e inflamatorias graves, que se hallan en consonancia con las características genéticas expresadas en el complejo enzimático dañado. El diagnóstico de la enfermedad resulta más confiable, exhaustivo y específico, mediante la citometría de flujo y su prueba de 1,2,3-dihidrorodamina(AU)


Introduction: Chronic granulomatous disease is a congenital primary immunodeficiency of the innate immune system, caused by defects in the nicotinamide adenine dinucleotide phosphate oxidase enzyme complex present in phagocytic cells. These functional defects cause inability to produce reactive oxygen species in phagocytes, affecting the elimination of some pathogenic microorganisms within the phagolysosome. The diagnosis of this disease is currently made by means of the 1,2,3-dihydrorodamine test assisted by multiparametric flow cytometry, or the staining of phagocytes with nitro-blue tetrazolium assisted by light microscopy. Objectives: To characterize molecular and pathophysiologically the chronic granulomatous disease; and to discuss aspects related to the aforementioned diagnostic tests. Methods: In this work, a bibliographic-documentary research was carried out from scientific articles published from 1933 to 2018, for which the SciELO, PubMed and Springer databases were consulted. Development: The pathophysiological characteristics of chronic granulomatous disease are exposed, as well as the relationship between the most abundant genetic mutations in the affected population, and the severity of the clinical manifestations presented by the patients. In addition, the benefits and deficiencies of two techniques currently used to diagnose the disease are critically analyzed. Conclusions: Chronic granulomatous disease can generate severe immunological and inflammatory consequences, which are in line with the genetic characteristics expressed in the damaged enzyme complex. The diagnosis of the disease is more reliable, exhaustive and specific, using flow cytometry and its 1,2,3-dihydrorodamine test(AU)


Assuntos
Humanos , Espécies Reativas de Oxigênio , Testes Diagnósticos de Rotina , Nitroazul de Tetrazólio/uso terapêutico , Técnicas e Procedimentos Diagnósticos , Citometria de Fluxo/métodos , Doença Granulomatosa Crônica/fisiopatologia , Doença Granulomatosa Crônica/genética
3.
Medicine (Baltimore) ; 99(23): e20599, 2020 Jun 05.
Artigo em Inglês | MEDLINE | ID: mdl-32502033

RESUMO

Pediatricians are unfamiliar with chronic granulomatous disease (CGD) because of its rarity and paucity of available data, potentially leading to misdiagnosis, late treatments, and mortality. The main purpose of this study was to summarize the clinical manifestations and auxiliary examination findings of four children with CGD confirmed by genetic testing.This was a case series study of children hospitalized at the Pediatric Respiratory Department of Shandong Provincial Hospital. The clinical, laboratory, treatment, and prognosis data were analyzed.All 4 children were boys. Two were brothers. The children's age was from 34 days to 3 years and 2 months at disease onset. The manifestations were repeated pulmonary infection, lymphadenitis, skin infection, and granuloma formation. Pulmonary infections were common. Abnormal responses were common after BCG vaccination. Thoracic computed tomography (CT) mainly showed nodules and masses, while the consolidation area in CT images reduced slowly. No abnormalities in cellular immune functions and immunoglobulin were found. The disease in all four children was confirmed by genetic testing. Long-term antibiotics and anti-fungal drugs were needed to prevent bacterial and fungal infections.CGD should be considered in children with repeated severe bacterial and fungal infections. Abnormal responses after BCG vaccination and nodular or mass-shaped consolidation in thoracic CT images should hint toward CGD. Gene sequencing could provide molecular evidence for diagnosis. The treatments of CGD include the prevention and treatment of infections and complications. Immunologic reconstitution treatment is currently the only curative treatment for CGD.


Assuntos
Testes Genéticos/métodos , Doença Granulomatosa Crônica/fisiopatologia , Pré-Escolar , Progressão da Doença , Doença Granulomatosa Crônica/diagnóstico , Doença Granulomatosa Crônica/genética , Humanos , Lactente , Masculino , Tórax/diagnóstico por imagem , Tórax/patologia , Tomografia Computadorizada por Raios X
4.
Autoimmunity ; 52(7-8): 256-263, 2019.
Artigo em Inglês | MEDLINE | ID: mdl-31556326

RESUMO

Blau syndrome is an autosomal dominant rare disease caused by mutations in NOD2 gene. Less than 200 patients published with Blau Syndrome Worldwide. We reported a 41-year old female Turkish patient diagnosed as Blau syndrome. Granulomatous dermatitis and severe headache, as well as recurrent chest and pelvic pain have been present since she was 8 years old. Arthritis started when she was teenage, hypertension diagnosed when she was 20 and other symptoms also occurred during the lifetime (severe preeclampsia, ischemic stroke, recurrent hemiparesis, recurrent-transient-vision-loss and renal-artery-stenosis). Genomic DNA was isolated from peripheral blood and 12 genes sequenced in Autoinflammatory panel on IonTorrent-S5-NGS platform with Parseq-VariFind™AIPassay. NGS analysis showed 107 variants in in the index case, mainly benign with no strong association with Blau syndrome. Additionally, we identified one very rare missense mutation in NOD2 gene (c2803G>A, p.Val935Met) and in silico assessment of the mutation indicated possible pathogenic significance and strong association with Blau syndrome. In addition, we analyzed family members of the index case and identified the same mutation in NOD2 gene. The segregation analysis shows the presence of the same mutant allele in NOD2 gene in the index case affected sister, as well as in her son with arthralgia, while in her non affecter brother we didn't detect the Val935Met mutation in NOD2 gene. Blau Syndrome is known as a very rare disease, mainly caused by mutations in NOD2 gene. Missense mutation diagnosed in our case could be responsible for the phenotype of the index case. Our results indicate the importance of NGS testing and its major role in the detection of rare mutations that may responsible for the onset of autoinflammatory disorders.


Assuntos
Artrite/genética , Dermatite/genética , Doença Granulomatosa Crônica/genética , Cefaleia/genética , Mutação de Sentido Incorreto , Proteína Adaptadora de Sinalização NOD2/genética , Acidente Vascular Cerebral/genética , Sinovite/genética , Uveíte/genética , Adulto , Alelos , Artrite/diagnóstico , Artrite/fisiopatologia , Criança , Dermatite/diagnóstico , Dermatite/fisiopatologia , Éxons , Feminino , Expressão Gênica , Doença Granulomatosa Crônica/diagnóstico , Doença Granulomatosa Crônica/fisiopatologia , Cefaleia/diagnóstico , Cefaleia/fisiopatologia , Sequenciamento de Nucleotídeos em Larga Escala , Humanos , Masculino , Pessoa de Meia-Idade , Sarcoidose , Acidente Vascular Cerebral/diagnóstico , Acidente Vascular Cerebral/fisiopatologia , Sinovite/diagnóstico , Sinovite/fisiopatologia , Uveíte/diagnóstico , Uveíte/fisiopatologia
5.
J Pak Med Assoc ; 68(9): 1387-1390, 2018 Sep.
Artigo em Inglês | MEDLINE | ID: mdl-30317271

RESUMO

Chronic granulomatous disease (CGD) is the most common of the primary immunodeficiency in children. It is caused by single gene defect resulting in dysfunctional nicotinamide adenine dineucleotide phosphate (NADPH) oxidase complex causing recurrent bacterial and fungal infections. Here we present the case of a 9 year old boy who was a known case of CGD since three years of age. He presented with recent history of fever, left sided pain in the scapular region and difficulty in breathing. Chest imaging revealed developing left upper lobe consolidation and erosion of the 3rd posterior rib. The child underwent video assisted thoracoscopic surgery (VATS) and biopsy of the lesion. Histopathology revealed fungal hyphae which were confirmed to be Aspergillus nidulans on staining. He was successfully treated with voriconazole therapy. We will also review the literature on fungal osteomyelitis in CGD patients.


Assuntos
Aspergilose , Aspergillus nidulans/isolamento & purificação , Doença Granulomatosa Crônica , Pneumopatias , Osteomielite , Voriconazol/administração & dosagem , Antifúngicos/administração & dosagem , Aspergilose/diagnóstico , Aspergilose/fisiopatologia , Aspergilose/terapia , Biópsia/métodos , Criança , Doença Granulomatosa Crônica/diagnóstico , Doença Granulomatosa Crônica/fisiopatologia , Humanos , Pulmão/diagnóstico por imagem , Pulmão/patologia , Pneumopatias/microbiologia , Pneumopatias/fisiopatologia , Pneumopatias/terapia , Masculino , Osteomielite/diagnóstico , Osteomielite/microbiologia , Osteomielite/fisiopatologia , Osteomielite/terapia , Costelas/diagnóstico por imagem , Costelas/patologia , Cirurgia Torácica Vídeoassistida/métodos , Resultado do Tratamento
6.
Bull Exp Biol Med ; 165(3): 302-306, 2018 Jul.
Artigo em Inglês | MEDLINE | ID: mdl-29998442

RESUMO

Correlations between extracellular matrix components in mouse lungs were examined during various terms of BCG-induced granulomatosis (on postinfection days 3, 30, 60, 90, and 180). During the development of pathological process, the revealed dynamic interrelations between structural units of proteoglycans and hydroxyproline weakened. Most correlations were observed on postinfection day 180. They reflect the relationships not only between the structural units of proteoglycans but also between collagens, presumably determining the maximum degree of fibrosis at this period. The established correlations characterize the systemic nature of reactions in extracellular matrix and its versatile implications determined by the processes going on in the organs and tissues during the onset and development of generalized pathology.


Assuntos
Vacina BCG/efeitos adversos , Galactose/metabolismo , Glicosaminoglicanos/metabolismo , Doença Granulomatosa Crônica/metabolismo , Hidroxiprolina/metabolismo , Proteoglicanas/metabolismo , Ácidos Urônicos/metabolismo , Animais , Vacina BCG/administração & dosagem , Progressão da Doença , Matriz Extracelular/química , Doença Granulomatosa Crônica/induzido quimicamente , Doença Granulomatosa Crônica/fisiopatologia , Injeções Intravenosas , Pulmão/efeitos dos fármacos , Pulmão/metabolismo , Pulmão/fisiopatologia , Masculino , Camundongos , Camundongos Endogâmicos BALB C
7.
J Pediatric Infect Dis Soc ; 7(suppl_1): S2-S5, 2018 May 09.
Artigo em Inglês | MEDLINE | ID: mdl-29746675

RESUMO

Chronic Granulomatous Disease is one of the classic primary immunodeficiencies of childhood. While the incidence and severity of bacterial and fungal infections have been greatly reduced in this patient population, much remains to be learned about the pathophysiology of the disease, particularly for autoinflammatory manifestations. In this review, we examine the epidemiology, pathophysiology, and genetic basis for CGD.


Assuntos
Doença Granulomatosa Crônica , Mutação , Criança , Feminino , Doença Granulomatosa Crônica/epidemiologia , Doença Granulomatosa Crônica/genética , Doença Granulomatosa Crônica/fisiopatologia , Humanos , Masculino , NADPH Oxidases/fisiologia
8.
BMJ Case Rep ; 20182018 Apr 19.
Artigo em Inglês | MEDLINE | ID: mdl-29674398

RESUMO

A 15-year-old girl presented with erythema nodosum and mild abdominal complaints. Her intestinal granulomatous disease was erroneously diagnosed as Crohn's disease despite the fact that the possibility of tuberculosis was considered. The final diagnosis of tuberculosis was made only when an anti-tumour necrosis factor therapy resulted in further deterioration. The patient was treated with isoniazid, rifampin, pyrazinamide and ethambutol, with slow and steady clinical improvement until complete recovery was achieved.


Assuntos
Antituberculosos/administração & dosagem , Doença de Crohn/diagnóstico , Doença Granulomatosa Crônica , Enteropatias , Tuberculose Gastrointestinal , Adolescente , Diagnóstico Diferencial , Feminino , Doença Granulomatosa Crônica/diagnóstico , Doença Granulomatosa Crônica/etiologia , Doença Granulomatosa Crônica/fisiopatologia , Humanos , Enteropatias/diagnóstico , Enteropatias/etiologia , Enteropatias/fisiopatologia , Tomografia Computadorizada por Raios X/métodos , Resultado do Tratamento , Teste Tuberculínico/métodos , Tuberculose Gastrointestinal/complicações , Tuberculose Gastrointestinal/diagnóstico , Tuberculose Gastrointestinal/tratamento farmacológico , Tuberculose Gastrointestinal/fisiopatologia
9.
Adv Ther ; 34(12): 2543-2557, 2017 12.
Artigo em Inglês | MEDLINE | ID: mdl-29168144

RESUMO

Chronic granulomatous disease (CGD) is a primary immunodeficiency caused by defects in any of the five subunits of the NADPH oxidase complex responsible for the respiratory burst in phagocytic leukocytes. Patients with CGD are at increased risk of life-threatening infections with catalase-positive bacteria and fungi and inflammatory complications such as CGD colitis. The implementation of routine antimicrobial prophylaxis and the advent of azole antifungals has considerably improved overall survival. Nevertheless, life expectancy remains decreased compared to the general population. Inflammatory complications are a significant contributor to morbidity in CGD, and they are often refractory to standard therapies. At present, hematopoietic stem cell transplantation (HCT) is the only curative treatment, and transplantation outcomes have improved over the last few decades with overall survival rates now > 90% in children less than 14 years of age. However, there remains debate as to the optimal conditioning regimen, and there is question as to how to manage adolescent and adult patients. The current evidence suggests that myeloablative conditioning results is more durable myeloid engraftment but with increased toxicity and high rates of graft-versus-host disease. In recent years, gene therapy has been proposed as an alternative to HCT for patients without an HLA-matched donor. However, results to date have not been encouraging. with negligible long-term engraftment of gene-corrected hematopoietic stem cells and reports of myelodysplastic syndrome due to insertional mutagenesis. Multicenter trials are currently underway in the United States and Europe using a SIN-lentiviral vector under the control of a myeloid-specific promoter, and, should the trials be successful, gene therapy may be a viable option for patients with CGD in the future.


Assuntos
Terapia Genética , Doença Granulomatosa Crônica/fisiopatologia , Doença Granulomatosa Crônica/terapia , Transplante de Células-Tronco Hematopoéticas , Adolescente , Adulto , Idoso , Idoso de 80 Anos ou mais , Criança , Pré-Escolar , Europa (Continente) , Feminino , Doença Granulomatosa Crônica/diagnóstico , Humanos , Lactente , Masculino , Pessoa de Meia-Idade , Adulto Jovem
10.
J Immunol Res ; 2017: 8745254, 2017.
Artigo em Inglês | MEDLINE | ID: mdl-28251166

RESUMO

Chronic Granulomatous Disease (CGD) is a rare inherited primary immunodeficiency, which is characterized by recurrent infections due to defective phagocyte NADPH oxidase enzyme. Nowadays, little is known about Chinese CGD patients. Here we report 48 CGD patients in our single center study, which is the largest cohort study from Mainland China. The ratio of male to female was 11 : 1. The mean onset age was 0.29 years old, and 52% patients had an onset within the 1st month of life. The mean diagnosis age was 2.24 years old. 11 patients (23%) had died with an average age of 2.91 years old. 13 patients (28%) had positive family histories. The most prevalent infectious sites were the lungs (77%), followed by gastrointestinal tract (54%), lymph nodes (50%), and skin (46%). In addition, septicopyemia, thrush, and hepatosplenomegaly were also commonly observed, accounting for 23%, 23%, and 40% of the cases. Lesions due to BCG vaccination occurred in more than half of the patients. X-linked CGD due to CYBB gene mutations accounted for 75% of the cases, and 11 of them were novel mutations. Autosomal recessive inheritance accounted for 6% patients, including 1 patient with CYBA, 1 with NCF1, and 1 with NCF2 gene mutations.


Assuntos
Doença Granulomatosa Crônica/genética , Doença Granulomatosa Crônica/fisiopatologia , Mutação , Adolescente , Adulto , Idoso , Vacina BCG/efeitos adversos , Criança , Pré-Escolar , China/epidemiologia , Estudos de Coortes , Feminino , Trato Gastrointestinal/microbiologia , Testes Genéticos , Doença Granulomatosa Crônica/congênito , Doença Granulomatosa Crônica/epidemiologia , Humanos , Síndromes de Imunodeficiência/genética , Lactente , Pulmão/microbiologia , Linfonodos/microbiologia , Masculino , Glicoproteínas de Membrana/genética , Pessoa de Meia-Idade , NADPH Oxidase 2 , NADPH Oxidases/genética , Pele/microbiologia , Adulto Jovem
11.
Rev Chil Pediatr ; 88(1): 136-141, 2017 02.
Artigo em Espanhol | MEDLINE | ID: mdl-28288231

RESUMO

Primary immunodeficiency diseases (PID) are congenital disorders secondary to an impaired immune response. Infections, autoimmune disorders, atopy, and lymphoproliferative syndromes are commonly associated with this disorder. OBJECTIVE: To present and discuss 3 infants diagnosed with PID. CLINICAL CASES: The cases are presented of three patients with PID diagnosed during their first admission to a Paediatric Intensive Critical Care Unit. The first patient, a 4-month-old infant affected by a severe pneumonia, and was diagnosed as a Severe Combined Immunodeficiency Disease. The second patient was an 8-month-old infant with Candida lusitaniae mesenteric adenitis, and diagnosed with a Chronic Granulomatous Disease. The last patient, a 6-month-old infant presented with ecthyma gangrenosum and X-linked agammaglobulinaemia. CONCLUSION: PID should be suspected when an infectious disease does not responde to the appropriate therapy within the expected period. An update of each disease is presented.


Assuntos
Agamaglobulinemia/diagnóstico , Doenças Genéticas Ligadas ao Cromossomo X/diagnóstico , Doença Granulomatosa Crônica/diagnóstico , Síndromes de Imunodeficiência/diagnóstico , Agamaglobulinemia/imunologia , Agamaglobulinemia/fisiopatologia , Doenças Genéticas Ligadas ao Cromossomo X/imunologia , Doenças Genéticas Ligadas ao Cromossomo X/fisiopatologia , Doença Granulomatosa Crônica/imunologia , Doença Granulomatosa Crônica/fisiopatologia , Humanos , Síndromes de Imunodeficiência/imunologia , Síndromes de Imunodeficiência/fisiopatologia , Lactente , Unidades de Terapia Intensiva Pediátrica , Masculino , Índice de Gravidade de Doença
12.
Ophthalmologica ; 235(1): 18-25, 2016.
Artigo em Inglês | MEDLINE | ID: mdl-26536452

RESUMO

PURPOSE: The aim of this study was to compare the outcomes of vitrectomy in granulomatous uveitis and nongranulomatous uveitis insufficiently managed by immunosuppressive therapy. METHODS: Thirty-eight eyes with granulomatous uveitis and 17 eyes with nongranulomatous uveitis that underwent vitrectomy for ocular complications between July 2006 and August 2012 were reviewed retrospectively. Visual acuity and ocular inflammation scores before and 6 months after surgery were compared. Patients treated with vitrectomy alone and those in whom vitrectomy was combined with phacoemulsification were analyzed separately. RESULTS: The mean visual acuity improved significantly both in granulomatous and nongranulomatous uveitis. In granulomatous uveitis, the mean inflammation scores decreased significantly both in the anterior segment and in the posterior segment. In nongranulomatous uveitis, the mean inflammation score in the posterior segment decreased significantly, although it did not change in the anterior segment. CONCLUSION: Vitrectomy was effective for treating ocular complications both in granulomatous uveitis and nongranulomatous uveitis, with favorable outcomes of improved visual acuity and decreased uveitis activity.


Assuntos
Doença Granulomatosa Crônica/cirurgia , Uveíte/cirurgia , Vitrectomia/métodos , Adulto , Idoso , Artrite/fisiopatologia , Artrite/cirurgia , Síndrome de Behçet/fisiopatologia , Síndrome de Behçet/cirurgia , Feminino , Doença Granulomatosa Crônica/fisiopatologia , Humanos , Iridociclite/fisiopatologia , Iridociclite/cirurgia , Masculino , Microcirurgia/métodos , Pessoa de Meia-Idade , Facoemulsificação , Estudos Retrospectivos , Sarcoidose/fisiopatologia , Sarcoidose/cirurgia , Resultado do Tratamento , Uveíte/fisiopatologia , Acuidade Visual/fisiologia
13.
Rev Chil Pediatr ; 86(2): 112-6, 2015.
Artigo em Espanhol | MEDLINE | ID: mdl-26235691

RESUMO

INTRODUCTION: Chronic granulomatous disease (CGD) is a rare form of primary immunodeficiency disease, characterized by an abnormal susceptibility to bacterial and fungal infections, and it is caused by a deficit in the phagocyte nicotinamide adenine dinucleotide phosphate oxidase complex (NADPH), resulting in the inability to generate reactive oxygen species that destroy microorganisms. The diagnosis is based on clinical characteristics and analysis of phagocytes, and later confirmed by molecular studies. Its management should consider antimicrobial prophylaxis, a search for infections and aggressive management of these. OBJECTIVE: To describe three cases of CGD emphasizing their forms of presentation and to conduct a review of the condition. CASE REPORTS: Three case reports, two of them first cousins, are presented. Molecular diagnosis was reached in one of the cases. Recurrent infections, abscesses, adenitis, granulomas and complications are identified to facilitate the suspected diagnosis of CGD, bearing in mind the importance of early diagnosis and genetic counseling. CONCLUSIONS: EGC is a rare congenital primary immunodeficiency disorder, mostly with X-linked inheritance, autosomal recessive form, and a specific presentation form. Its diagnosis should be timely to avoid complications. Prophylaxis and aggressive treatment of infections should be performed, as well as genetic counseling.


Assuntos
Doença Granulomatosa Crônica/diagnóstico , Técnicas de Diagnóstico Molecular/métodos , Fagócitos/metabolismo , Adolescente , Criança , Feminino , Aconselhamento Genético/métodos , Doença Granulomatosa Crônica/genética , Doença Granulomatosa Crônica/fisiopatologia , Humanos , Lactente , Masculino
14.
Indian Heart J ; 67(3): 222-6, 2015.
Artigo em Inglês | MEDLINE | ID: mdl-26138178

RESUMO

BACKGROUND: Granulomatous myocarditis may present with sustained monomorphic ventricular tachycardia (SMVT) in the presence of normal left ventricular ejection fraction (LVEF), and could be mistaken for idiopathic ventricular tachycardia (IVT). The use of cardiac imaging for diagnosis can be limited by availability and high cost. ECG is readily available and inexpensive. Fragmented QRS (fQRS) on ECG has been found to be associated with myocardial scar. We hypothesized that fQRS could be useful in the diagnosis of granulomatous VT (GVT). METHODS: We compared the 12-lead ECG of 16 patients with GVT and 42 patients with IVT who presented with SMVT. RESULTS: The presence of fQRS was significantly higher in the GVT group compared to the IVT group (75% versus 19.1%, p < 0.001). The location of fQRS correlated with delayed enhancement cardiac magnetic resonance imaging (DE-CMR) in the same segment in 4/16 patients in the GVT group. It correlated with an affected segment on either DE-CMR or 18FDG positron emission computed tomography in 4/11 patients in the GVT group who had both imaging modality. Whenever fQRS was present in contiguous leads other than the inferior leads, it always corresponded to an affected segment on imaging. CONCLUSIONS: In patients presenting with SMVT and no structural heart disease, the presence of fQRS is strongly associated with granulomatous myocarditis. fQRS on the surface ECG is a helpful tool the presence of which should prompt a CMR for a definitive diagnosis.


Assuntos
Diagnóstico por Imagem/métodos , Eletrocardiografia , Doença Granulomatosa Crônica/diagnóstico , Volume Sistólico/fisiologia , Taquicardia Ventricular/etiologia , Função Ventricular Esquerda/fisiologia , Adulto , Feminino , Seguimentos , Doença Granulomatosa Crônica/complicações , Doença Granulomatosa Crônica/fisiopatologia , Humanos , Imagem Cinética por Ressonância Magnética , Masculino , Tomografia por Emissão de Pósitrons , Estudos Retrospectivos , Taquicardia Ventricular/diagnóstico , Taquicardia Ventricular/fisiopatologia
15.
Rev. chil. pediatr ; 86(2): 112-116, abr. 2015. ilus
Artigo em Espanhol | LILACS | ID: lil-752888

RESUMO

Introducción: La enfermedad granulomatosa crónica (EGC) es una forma infrecuente de inmunodeficiencia primaria que se caracteriza por una sensibilidad anormal a infecciones bacterianas y fúngicas, debida a un déficit en el complejo nicotinamida adenina dinucleótida fosfato oxidasa (NADPH) en los fagocitos. Objetivo: Describir tres casos de EGC con énfasis en su forma de presentación y realizar una revisión del tema. Casos Clínicos: Se presentan tres casos clínicos, dos de ellos con relación de parentesco (primos en primer grado). Se llegó a diagnóstico molecular en uno de los casos. Se destacan las manifestaciones clínicas: infecciones recurrentes, abscesos, adenitis y granulomas, y complicaciones, con la finalidad de facilitar la sospecha diagnóstica de EGC, debido a la importancia del diagnóstico temprano y el consejo genético. Conclusiones: La EGC es un trastorno inmunológico primario congénito infrecuente, con herencia ligada a X en su mayoría, pero también con formas autosómicas recesivas, con una forma de presentación característica y cuyo diagnóstico debe ser oportuno para evitar complicaciones, realizar profilaxis y tratamiento agresivo de las infecciones y consejo genético.


Introduction: Chronic granulomatous disease (CGD) is a rare form of primary immunodeficiency disease, characterized by an abnormal susceptibility to bacterial and fungal infections, and it is caused by a deficit in the phagocyte nicotinamide adenine dinucleotide phosphate oxidase complex (NADPH), resulting in the inability to generate reactive oxygen species that destroy micro-organisms. The diagnosis is based on clinical characteristics and analysis of phagocytes, and later confirmed by molecular studies. Its management should consider antimicrobial prophylaxis, a search for infections and aggressive management of these. Objective: To describe three cases of CGD emphasizing their forms of presentation and to conduct a review of the condition. Case reports: Three case reports, two of them first cousins, are presented. Molecular diagnosis was reached in one of the cases. Recurrent infections, abscesses, adenitis, granulomas and complications are identified to facilitate the suspected diagnosis of CGD, bearing in mind the importance of early diagnosis and genetic counseling. Conclusions: EGC is a rare congenital primary immunodeficiency disorder, mostly with X-linked inheritance, autosomal recessive form, and a specific presentation form. Its diagnosis should be timely to avoid complications. Prophylaxis and aggressive treatment of infections should be performed, as well as genetic counseling.


Assuntos
Humanos , Masculino , Feminino , Lactente , Criança , Adolescente , Fagócitos/metabolismo , Técnicas de Diagnóstico Molecular/métodos , Doença Granulomatosa Crônica/diagnóstico , Aconselhamento Genético/métodos , Doença Granulomatosa Crônica/fisiopatologia , Doença Granulomatosa Crônica/genética
16.
J Am Heart Assoc ; 3(3): e000920, 2014 Jun 27.
Artigo em Inglês | MEDLINE | ID: mdl-24973227

RESUMO

BACKGROUND: In vitro study showed that NADPH oxidase (NOx), the most important enzyme producing reactive oxygen species (ROS), plays a role in the process of platelet activation. However, it is unclear if changes in its activity affect platelet activation in vivo. METHODS AND RESULTS: In vivo and ex vivo experiments assessing platelet activation were investigated in healthy subjects, obese patients, and subjects with different low rates of NOx2 activity, namely X-linked chronic granulomatous disease (X-CGD) patients and X-CGD carriers. We included 27 X-CGD patients, 31 women carriers of hereditary deficiency of NOx2, 31 obese women, and 62 healthy subjects matched for sex and age. Plasma levels of soluble sCD40 L (sCD40L) and soluble P (sP)-selectin, 2 markers of in vivo platelet activation, were reduced in X-CGD patients (sCD40L=-55%; sP-selectin=-51%, P<0.001) and in X-CGD carriers (sCD40L=-41%; sP-selectin=-57%, P<0.001) compared with respective controls. Conversely, obese women, who disclosed NOx2 upregulation, had significantly higher plasma levels of sCD40L (+47%, P<0.001) and sP-selectin (+70%, P<0.001) compared with controls. Ex vivo study showed platelet isoprostane downexpression and enhanced platelet NO generation in both X-CGD patients and X-CGD carriers compared with controls; opposite findings were observed in obese patients. Correlation analysis showed that platelet NOx2 regulation was directly associated with plasma levels of sCD40L (R=0.336, P<0.001) and sP-selectin (R=0.441; P<0.001). CONCLUSIONS: The study provides the first evidence that in vivo platelet activation is significantly and directly associated with NOx2 activity. Platelet NOx2 may be a novel target for platelet activation inhibition.


Assuntos
Doença Granulomatosa Crônica/enzimologia , Glicoproteínas de Membrana/fisiologia , NADPH Oxidases/fisiologia , Ativação Plaquetária/fisiologia , Adolescente , Adulto , Plaquetas/enzimologia , Ligante de CD40/sangue , Estudos de Casos e Controles , Criança , Feminino , Doença Granulomatosa Crônica/sangue , Doença Granulomatosa Crônica/fisiopatologia , Heterozigoto , Humanos , Masculino , Glicoproteínas de Membrana/metabolismo , NADPH Oxidase 2 , NADPH Oxidases/metabolismo , Obesidade/sangue , Obesidade/enzimologia , Obesidade/fisiopatologia , Selectina-P/sangue
17.
Rev Chil Pediatr ; 85(2): 213-21, 2014 Apr.
Artigo em Espanhol | MEDLINE | ID: mdl-25697211

RESUMO

UNLABELLED: Chronic granulomatous disease (CGD) is caused by mutations in the genes that encode five of the subunits of the human NADPH oxidase. The most common form is caused by mutations in CYBB, the human gene encoding gp 91 phox. OBJECTIVE: To identify the molecular defects causing CGD. CASE REPORT: A male patient with a history of acute diarrhea and recurrent perianal abscess since two months old. At 6 months, the patient presented a chronic inflammatory disease of the colon and bacterial colitis. After three years, he developed infections in the lower and perianal respiratory tract. The cDNA analysis identified abnormal mRNA expression, which was confirmed by sequencing. Specifically the exclusion of exon 2 was observed. Additionally, gDNA sequencing identified an alteration in the acceptor splice site of intron 1, including a deletion followed by insertion of three nucleotides (c.46-14_-11delTTCT insGAA). CONCLUSIONS: The first molecular study of a patient with CGD due to splicing pattern change, reported in Colombia, is presented. The definition of the mutation and its correlation with the phenotype is essential to provide appropriate genetic counseling to patients and their families.


Assuntos
Doença Granulomatosa Crônica/genética , NADPH Oxidases/genética , Splicing de RNA/genética , Pré-Escolar , Colômbia , Éxons , Doença Granulomatosa Crônica/fisiopatologia , Humanos , Íntrons , Masculino , Mutação , Fenótipo , Sítios de Splice de RNA , RNA Mensageiro/genética , Análise de Sequência de DNA , Análise de Sequência de RNA
19.
Antioxid Redox Signal ; 18(12): 1491-6, 2013 Apr 20.
Artigo em Inglês | MEDLINE | ID: mdl-23216310

RESUMO

NADPH oxidase is known to modulate the arterial tone, but the role of its specific subunits is still unclear. The objective of this study was to compare the role of p47 and gp91phox (NOX2) on artery dilatation. We conducted a multicenter study enrolling 30 patients with chronic granulomatous disease (CGD) (25 with NOX2 deficiency and 5 with p47(phox) deficiency) and 30 healthy subjects (HS), matched for gender and age, in whom flow-mediated dilation (FMD), serum activity of NOX2 (soluble NOX2-derived peptide [sNOX2-dp]), urinary isoprostanes (8-iso-PGF2α), and platelet production of isoprostanes and NOX2 were determined. Compared to HS, patients with CGD had significantly higher FMD and lower sNOX2-dp and 8-iso-PGF2α levels. Compared to patients with NOX2 deficiency and HS, patients with p47(phox) hereditary deficiency had intermediate FMD and oxidative stress, that is, higher and lower FMD and lower and higher isoprostanes compared to HS and patients with NOX2 deficiency, respectively. In agreement with this finding, an ex vivo study showed higher inhibition of NOX2 activity and lower isoprostane formation in platelets from patients with NOX2 deficiency compared to platelets from ones with p47(phox) deficiency. Our observations lead to the hypothesis that oxidants are implicated in artery vasoconstriction.


Assuntos
Doença Granulomatosa Crônica/fisiopatologia , Glicoproteínas de Membrana/deficiência , NADPH Oxidases/deficiência , Vasodilatação , Adolescente , Plaquetas/enzimologia , Artérias Carótidas/diagnóstico por imagem , Artérias Carótidas/patologia , Espessura Intima-Media Carotídea , Estudos de Casos e Controles , Criança , Feminino , Doença Granulomatosa Crônica/enzimologia , Doença Granulomatosa Crônica/urina , Humanos , Isoprostanos/urina , Masculino , NADPH Oxidase 2 , Adulto Jovem
20.
Swiss Med Wkly ; 142: w13602, 2012.
Artigo em Inglês | MEDLINE | ID: mdl-22714396

RESUMO

The monogenic autoinflammatory syndromes are conditions caused by mutations of genes coding for proteins that play a pivotal role in the regulation of the inflammatory response. Due to their genetic nature, most of these disorders have an early onset. Clinically they are characterised by recurrent flares of systemic inflammation presenting most of the time as sudden fever episodes associated with elevation of acute phase reactants and with a number of clinical manifestations such as rash, serositis, lymphadenopathy and arthritis. Symptom-free intervals are characterised by complete wellbeing, normal growth and complete normalisation of acute phase reactants. Familial Mediterranean fever (FMF), mevalonate-kinase deficiency (MKD) and tumour necrosis factor (TNF) receptor-associated periodic syndrome (TRAPS) are the three monogenic disorders subsumed under the term periodic fevers, while a systemic inflammation dominated by a characteristic urticarial rash associated with a number of other clinical manifestations is typical of familial cold autoinflammatory syndrome (FCAS), Muckle-Wells syndrome (MWS) and chronic infantile neurological cutaneous and articular syndrome (CINCA). These diseases represent the clinical spectrum of different mutations of a gene named cold-induced autoinflammatory syndrome 1 (CIAS-1, or NLRP3) coding for a protein called cryopyrin. Hence these disorders are also known as cryopyrin-associated periodic syndromes (CAPS). Other conditions are characterised by typical granulomatous formations (granulomatous disorders). Blau's syndrome (familial juvenile systemic granulomatosis) presents with non-caseating granulomatous inflammation affecting the joint, skin, and uveal tract (the triad of arthritis, dermatitis and uveitis) and is associated with mutations of the NACHT domain of the gene CARD15 (or NOD2).


Assuntos
Doença Granulomatosa Crônica/genética , Doenças Hereditárias Autoinflamatórias/genética , Doença Granulomatosa Crônica/fisiopatologia , Doença Granulomatosa Crônica/terapia , Doenças Hereditárias Autoinflamatórias/fisiopatologia , Doenças Hereditárias Autoinflamatórias/terapia , Humanos , Inflamação/genética
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