RESUMO
BACKGROUND: Microsporidia are common opportunistic parasites in humans and animals, including rabbits. However, only limited epidemiology data concern about the prevalence and molecular characterization of Enterocytozoon bieneusi and Encephalitozoon spp. in rabbits. This study is the first detection and genotyping of Microsporidia in pet rabbits in China. RESULTS: A total of 584 faecal specimens were collected from rabbits in pet shops from four cities in Sichuan province, China. The overall prevalence of microsporidia infection was 24.8% by nested PCR targeting the internal transcribed spacer (ITS) region of E. bieneusi and Encephalitozoon spp. respectively. E. bieneusi was the most common species (n = 90, 15.4%), followed by Encephalitozoon cuniculi (n = 34, 5.8%) and Encephalitozoon intestinalis (n = 16, 2.7%). Mixed infections (E. bieneusi and E. cuniculi) were detected in five another rabbits (0.9%). Statistically significant differences in the prevalence of microsporidia were observed among different cities (χ2 = 38.376, df = 3, P < 0.01) and the rabbits older than 1 year were more likely to harbour microsporidia infections (χ2 = 9.018, df = 2, P < 0.05). Eleven distinct genotypes of E. bieneusi were obtained, including five known (SC02, I, N, J, CHY1) and six novel genotypes (SCR01, SCR02, SCR04 to SCR07). SC02 was the most prevalent genotype in all tested cities (43.3%, 39/90). Phylogenetic analysis showed that these genotypes were clustered into group 1-3 and group 10. Meanwhile, two genotypes (I and II) were identified by sequence analysis of the ITS region of E. cuniculi. CONCLUSION: To the best of our knowledge, this is the first report of microsporidia infection in pet rabbits in China. Genotype SC02 and four novel genotypes were classified into potential zoonotic group 1, suggesting that pet rabbits may cause microsporidiosis in humans through zoonotic transmissions. These findings provide preliminary reference data for monitoring microsporidia infections in pet rabbits and humans.
Assuntos
Encephalitozoon/isolamento & purificação , Enterocytozoon/isolamento & purificação , Microsporidiose/veterinária , Animais , China/epidemiologia , Encephalitozoon/classificação , Encephalitozoon/genética , Enterocytozoon/classificação , Enterocytozoon/genética , Fezes/microbiologia , Genótipo , Microsporidiose/epidemiologia , Reação em Cadeia da Polimerase/veterinária , Prevalência , CoelhosRESUMO
Intestinal microsporidiosis is known as an opportunistic infection in immunocompromised patients. The current study aimed to investigate intestinal microsporidia infection in human subjects with/without immunodeficiency. Totally, 600 stool samples were collected from immunocompromised (254) and immunocompetent (346) subjects. DNA extraction was performed and the SSU rRNA and the ITS genes were amplified to detect and characterize microsporidia and the relevant genotypes. Phylogenetic trees were drawn using MEGA7 software to illustrate the correlation between isolates. From 600 enrolled subjects, 283 and 317 were male and female, respectively. The average age ± SD of all tested subjects was 28.85 ± 26.92. The results of PCR demonstrated the presence of E. bieneusi and Encephalitozoon sp., among 10/600 (1.67%) and 26/600 (4.33%) of samples, respectively. Accordingly, E. bieneusi was seen among 4/346 (1.15%), 1/53 (1.88%), 3/124 (2.42%), and 2/63 (3.17%), and Encephalitozoon sp., was detected from 17/346 (4.91%), 3/53 (5.36%), 4/124 (3.22%) and 2/63 (3.17%) of healthy subjects, RA patients, cancer patients, and transplantation recipients, respectively. Statistical significant correlation was not seen between the presence of microsporidia and age, gender, stool appearance, and geographical region. Molecular analysis showed that all E. bieneusi were the genotype D. Phylogenetic tree demonstrated no classification according to the presence/absence of immunodeficiency, geographical locations and presence of diarrhea. The high prevalence of Encephalitozoon sp., in comparison to E. bieneusi in this study suggested the importance of this genus alongside with E. bieneusi in Iran. In addition, predominance of the genotype D highlighted the wide distribution of this genotype in Iran.
Assuntos
Encephalitozoon , Enterocytozoon , Microsporidiose/epidemiologia , Adolescente , Adulto , Idoso , Criança , Pré-Escolar , DNA Espaçador Ribossômico , Encephalitozoon/classificação , Encephalitozoon/genética , Encephalitozoon/isolamento & purificação , Enterocytozoon/classificação , Enterocytozoon/genética , Enterocytozoon/isolamento & purificação , Feminino , Genes Fúngicos , Humanos , Hospedeiro Imunocomprometido , Irã (Geográfico)/epidemiologia , Masculino , Pessoa de Meia-Idade , Epidemiologia Molecular , Infecções Oportunistas/epidemiologia , Patologia Molecular/métodos , Filogenia , RNA Ribossômico 18S , Adulto JovemRESUMO
Cryptosporidium spp. Enterocytozoon bieneusi and Encephalitozoon intestinalis are opportunistic pathogens responsible for gastrointestinal diseases. We evaluated the ParaGENIE Crypto-Micro Real-Time PCR kit (Ademtech, France), the first CE-IVD compliant PCR assay available for these pathogens. This study was conducted blindly against a reference panel of 115 stool specimens including positive samples for Cryptosporidium spp. (nâ¯=â¯48) and E. bieneusi (nâ¯=â¯38) as well as negative or positive samples for other parasites to test for cross-reactivity. An additional set of samples corresponding to 8 rare Cryptosporidium species was also included. Discrepancies were evaluated with external in-house PCR tests. The ParaGENIE Crypto-Micro PCR assay displayed a sensitivity/specificity of 91.7%/100% and 97.3%/98.7% for Cryptosporidium spp. and E. bieneusi, respectively, and was able to detect all 12 Cryptosporidium species of the reference panel, including rare species. This new CE-IVD assay will facilitate the diagnosis of intestinal cryptosporidiosis and microsporidiosis, a major concern in immunocompromised patients and travelers.
Assuntos
Técnicas Bacteriológicas/métodos , Cryptosporidium/genética , Encephalitozoon/genética , Enterocytozoon/genética , Fezes/microbiologia , Fezes/parasitologia , Reação em Cadeia da Polimerase Multiplex , Cryptosporidium/classificação , DNA Fúngico/genética , DNA de Protozoário/genética , Encephalitozoon/classificação , Enterocytozoon/classificação , Humanos , Kit de Reagentes para Diagnóstico , Sensibilidade e EspecificidadeRESUMO
Microsporidia are opportunistic pathogens that infect a wide range of invertebrates and vertebrates. To assess the potential role of dogs in the transmission of these zoonotic pathogens, a total of 282 fecal samples from dogs in the Central Anatolia Region of Turkey were analyzed by utilizing species specific polymerase chain reaction for the four most frequent human microsporidia. Two microsporidia species were recognized in 41 samples (14.5%). Encephalitozoon intestinalis was detected in 35 samples (12.4%) and it was the most common microsporidium. The second microsporidium, E. cuniculi, was identified in six (2.1%) of the samples. Sequence analysis of the intergenic spacer of the ribosomal ribonucleic acid (RNA) internal transcribed spacer (ITS) gene revealed the presence of three E. intestinalis haplotypes closely associated with each other. No polymorphic region was found among the ITS sequences of E. cuniculi isolates and they were characterized as genotype III. This study provides the first data on the zoonotic microsporidia species from dogs in Turkey.
Assuntos
Doenças do Cão/microbiologia , Encephalitozoon/isolamento & purificação , Encefalitozoonose/microbiologia , Microsporídios/isolamento & purificação , Microsporidiose/microbiologia , Animais , Doenças do Cão/epidemiologia , Cães , Encephalitozoon/classificação , Encephalitozoon/genética , Encefalitozoonose/epidemiologia , Fezes/microbiologia , Genótipo , Humanos , Microsporídios/classificação , Microsporídios/genética , Microsporidiose/epidemiologia , Turquia/epidemiologia , Zoonoses/microbiologia , Zoonoses/transmissãoRESUMO
BACKGROUND : Microsporidia may cause infection in both immunocompromised and immunocompetent populations. The best strategy to control microsporidiosis is obtaining thorough knowledge of its outbreak and pathogenicity. PURPOSE : Because of the lack of precise estimation of microsporidia prevalence among Iranian children with cancer, the current study aimed at evaluating the rate of intestinal microsporidia in children undergoing chemotherapy. METHODS: Patients with cancer undergoing chemotherapy in a children's hospital in Northwestern Iran were studied; 132 stool samples were collected and stained by the Weber and Ryan-blue modified trichrome staining techniques. The extracted DNA samples were evaluated by the nested polymerase chain reaction (PCR) method. All positive isolates were sequenced for genotyping and phylogenetic analysis. RESULTS: A total of 17 (12.8%) samples were microscopically positive for microsporidia infection, whereas only 14 (10.6%) cases were positive based on nested PCR results. In the positive samples detected with nested PCR, the frequency of Enterocytozoon bieneusi and Encephalitozoon intestinalis infections was 71.4% (n = 10) and 28.6% (n = 4), respectively. After sequencing and phylogenetic analysis, the genotype of E. bieneusi was type D and the sequences of the isolated species were similar to those of the registered ones. CONCLUSION: E. bieneusi is a major contributor to microsporidiosis in young immunocompromised patients in Iran. Microsporidia species are well-detected when confirmatory techniques such as molecular methods are in agreement with staining. So, to ensure this, a suggestion has been made to introduce a certain diagnostic test for microsporidiosis.
Assuntos
Encephalitozoon/classificação , Encephalitozoon/isolamento & purificação , Encefalitozoonose/epidemiologia , Encefalitozoonose/microbiologia , Neoplasias/complicações , Infecções Oportunistas/epidemiologia , Infecções Oportunistas/microbiologia , Criança , DNA Fúngico/química , DNA Fúngico/isolamento & purificação , Encephalitozoon/genética , Encefalitozoonose/patologia , Fezes/microbiologia , Genótipo , Humanos , Irã (Geográfico) , Infecções Oportunistas/patologia , Filogenia , Reação em Cadeia da Polimerase , Prevalência , Análise de Sequência de DNARESUMO
Microsporidia are obligate intracellular parasites that produce spores. The infections caused by these parasites are mostly considered to be opportunistic in immunodeficient patients. Because of the zoonotic nature of microsporidia as well as the increasing prevalence of immunodeficiency diseases, the aim of this study was to evaluate the molecular diagnosis of Enterocytozoon bieneusi (E. bieneusi) and Encephalitozoon spp. in exotic birds in southwestern Iran. Initially, 816 stool specimens were collected and stained by modified trichrome (Weber) staining. The slides were explored using light microscopy. In the next stage, the extracted DNA was amplified using a multiplex/nested PCR method. RFLP with the Mnl1 restriction enzyme was used to differentiate the Encephalitozoon species in the products of the molecular analysis. Out of 816 samples, 138 and 181 cases were found to be positive by the staining and the multiplex/nested-PCR methods, respectively. Of the 181 samples, 103 and 78 samples were positive for E. bieneusi and Encephalitozoon spp., respectively. The Encephalitozoon species were 17 E. cuniculi, 52 E. intestinalis and 9 E. hellem. Of 103 E. bieneusi samples, 57, 39, 2 and 5 cases were detected as genotypes D, M, E and L, respectively. The results showed a relatively high prevalence of microsporidia in exotic birds, and according to the results of the genotyping, these birds can be an important source of microsporidiosis. It is essential that high-risk individuals, including patients with immunodeficiency diseases, receive accurate and valid information about the risk of direct and indirect contact with infected exotic birds.
Assuntos
Aves/microbiologia , Encephalitozoon/genética , Enterocytozoon/genética , Microsporidiose/diagnóstico , Microsporidiose/epidemiologia , Adulto , Animais , Animais Exóticos , Encephalitozoon/classificação , Encephalitozoon/isolamento & purificação , Enterocytozoon/classificação , Enterocytozoon/isolamento & purificação , Fezes/microbiologia , Técnicas de Genotipagem , Humanos , Irã (Geográfico)/epidemiologia , Masculino , Microsporidiose/microbiologia , Microsporidiose/transmissão , Reação em Cadeia da Polimerase , Polimorfismo de Fragmento de RestriçãoRESUMO
The microsporidium parasitizing Inland Bearded Dragons Pogona vitticeps, and developing primarily in macrophages within foci of granulomatous inflammation of different organs, is described as a new species Encephalitozoon pogonae. Establishing the new species was based on sequencing the ITS-SSUrDNA region of the ribosomal gene and consequent SSUrDNA-inferred phylogenetic analyses, as well as on comparison of pathogenesis, host specificity, and ultrastructure among Encephalitozoon species and isolates. The new species is closely related to E. lacertae and E. cuniculi. Analysis of the literature suggests that this microsporidium has been reported previously as an unidentified microsporidian species or isolate of E. cuniculi and may represent a common infection in bearded dragons. All stages of E. pogonae develop in parasitophorous vacuoles. Uninucleate spores on methanol-fixed smears measured 2.1 × 1.1 µm, range 1.7-2.6 × 0.9-1.7 µm; on ultrathin sections spores measured 0.8-1.1 × 1.8-2.2 µm. Ultrastructural study revealed 3-6 polar filament coils, a mushroom-shaped polar disk, and a polar sac embracing half of the volume occupied by the lamellar polaroplast. In activated spores, polar filament everted eccentrically. The overall morphology and intracellular development of E. pogonae were similar to other Encepahalitozoon spp. We also review the existing data on microsporidia infecting reptiles.
Assuntos
Encephalitozoon/genética , Encefalitozoonose/veterinária , Lagartos/microbiologia , Animais , Encephalitozoon/classificação , Encephalitozoon/isolamento & purificação , Encefalitozoonose/microbiologia , Microscopia Eletrônica de Varredura , Filogenia , Análise de Sequência de DNA , Esporos Fúngicos/ultraestruturaRESUMO
Increased risk of zoonotic transmission of the potential human pathogenic species Enterocytozoon bieneusi, Encephalitozoon intestinalis and Encephalitozoon cuniculi was detected in wild immunocompetent mice (Mus musculus musculus; n=280). Analysis was conducted with the use of PMP1/PMP2 primers and SYBR Green RT-PCR. Using Real Time PCR and comparing the sequences with sequences in the GenBank, E. bieneusi was detected in 3 samples (1.07 %), E. cuniculi in 1 sample (0.35 %) and E. intestinalis in 1 sample (0.35 %). The results of this report document the low host specificity of detected microsporidia species, and imply the importance of synanthropic rodents as a potential source of human microsporidial infection.
Assuntos
Encephalitozoon/isolamento & purificação , Enterocytozoon/isolamento & purificação , Camundongos , Microsporidiose/veterinária , Doenças dos Roedores/epidemiologia , Animais , Encephalitozoon/classificação , Encephalitozoon/genética , Encefalitozoonose/epidemiologia , Encefalitozoonose/parasitologia , Encefalitozoonose/veterinária , Enterocytozoon/classificação , Enterocytozoon/genética , Fezes/parasitologia , Microsporidiose/epidemiologia , Microsporidiose/parasitologia , Prevalência , Reação em Cadeia da Polimerase em Tempo Real/veterinária , Doenças dos Roedores/parasitologia , Roedores , Eslováquia/epidemiologiaRESUMO
BACKGROUND: Infectious diseases represent the greatest threats to endangered species, and transmission from humans to wildlife under increased anthropogenic pressure has been always stated as a major risk of habituation. AIMS: To evaluate the impact of close contact with humans on the occurrence of potentially zoonotic protists in great apes, one hundred mountain gorillas (Gorilla beringei beringei) from seven groups habituated either for tourism or for research in Volcanoes National Park, Rwanda were screened for the presence of microsporidia, Cryptosporidium spp. and Giardia spp. using molecular diagnostics. RESULTS: The most frequently detected parasites were Enterocytozoon bieneusi found in 18 samples (including genotype EbpA, D, C, gorilla 2 and five novel genotypes gorilla 4-8) and Encephalitozoon cuniculi with genotype II being more prevalent (10 cases) compared to genotype I (1 case). Cryptosporidium muris (2 cases) and C. meleagridis (2 cases) were documented in great apes for the first time. Cryptosporidium sp. infections were identified only in research groups and occurrence of E. cuniculi in research groups was significantly higher in comparison to tourist groups. No difference in prevalence of E. bieneusi was observed between research and tourist groups. CONCLUSION: Although our data showed the presence and diversity of important opportunistic protists in Volcanoes gorillas, the source and the routes of the circulation remain unknown. Repeated individual sampling, broad sampling of other hosts sharing the habitat with gorillas and quantification of studied protists would be necessary to acquire more complex data.
Assuntos
Doenças dos Símios Antropoides/epidemiologia , Criptosporidiose/epidemiologia , Encefalitozoonose/epidemiologia , Giardíase/epidemiologia , Zoonoses/epidemiologia , Animais , Doenças dos Símios Antropoides/microbiologia , Doenças dos Símios Antropoides/parasitologia , Criptosporidiose/parasitologia , Cryptosporidium/classificação , Cryptosporidium/genética , Cryptosporidium/isolamento & purificação , DNA Intergênico/genética , Encephalitozoon/classificação , Encephalitozoon/genética , Encephalitozoon/isolamento & purificação , Encefalitozoonose/microbiologia , Giardia/classificação , Giardia/genética , Giardia/isolamento & purificação , Giardíase/parasitologia , Hominidae , Dados de Sequência Molecular , Parques Recreativos , Filogenia , Ruanda/epidemiologia , Zoonoses/microbiologia , Zoonoses/parasitologiaRESUMO
Cryptosporidium spp. and microsporidia are opportunistic parasites affecting a wide range of hosts in which they can be potentially life threatening in immunocompromised individuals. Diagnosis usually relies on the identification of the stained Cryptosporidium oocyst or microsporidial spores, but these methods lack sensitivity and require highly trained technicians to perform and interpret the results. Molecular diagnosis offers an alternative with both superior sensitivity and specificity as compared to microscopy. Although replacing microscopy with nucleic acid based methods is hampered by the higher costs, in particular in developing countries, multiplexing the detection of more than one parasite in a single test has been found to be very effective and would decrease the cost of the test without the need for new equipment, as it would be the case for quantitative PCR. The method shown in this report for the simultaneous detection of Cryptosporidium spp., Enterocytozoon bieneusi and Encephalitozoon intestinalis by multiplex nested PCR, has proved to have several advantages versus microscopy such as higher sensitivity and specificity, low subjectivity and a minimal need for specialist's training to interpret the results. The present multiplex assay can fill an important gap to identify other possible causative agents of several diarrheal diseases which until present remain undiagnosed and can improve the epidemiology of the disease with a more reliable detection method.
Assuntos
Cryptosporidium/genética , Encephalitozoon/genética , Enterocytozoon/genética , Reação em Cadeia da Polimerase/métodos , Cryptosporidium/classificação , DNA Fúngico/genética , DNA de Protozoário/genética , Encephalitozoon/classificação , Enterocytozoon/classificação , Sensibilidade e Especificidade , Especificidade da EspécieRESUMO
Microsporidia are ubiquitous fungi with genomes that have undergone a strong reduction to the extreme cases of Encephalitozoon cuniculi and Encephalitozoon intestinalis. Genetic variability within species of the Encephalitozoon genus has been reported, with most of the studies based on the internal transcribed spacer (ITS) of the rDNA. However, in contrast to the picture of E. cuniculi and Encephalitozoon hellem, where different strains have been identified, no genetic variability has yet been observed in E. intestinalis. We have analysed tandem repeats included in putative coding sequences which could be used as polymorphic markers in E. intestinalis. Eight candidate loci (M2, M2A, M3, M5, M7, M7A, M8 and PTP1) were established and 9 E. intestinalis cultured strains from North America, South America and Europe were analysed. M2, M7 and PTP1 nucleotide sequences were identical among the different strains and the GenBank sequence. In contrast, we observed variants in 4 markers (M2A, M3, M7A and M8) which did not correspond to their respective reference sequences. The most noticeable finding was that with the M5 marker two genotypes were defined among the different strains studied, demonstrating genotypic variability of E. intestinalis. Although the diversity described is certainly not high, which can be explained by a lower chance of genetic variability in its minimal genome, we have demonstrated that polymorphisms actually exist in E. intestinalis. Epidemiological studies using this genetic marker should now be conducted to elucidate the genetic variability in E. intestinalis and improve our knowledge of the epidemiology of this microsporidia.
Assuntos
DNA Fúngico/genética , Encephalitozoon/genética , Sequências de Repetição em Tandem/genética , Animais , Sequência de Bases , Encephalitozoon/classificação , Encefalitozoonose/parasitologia , Proteínas Fúngicas/genética , Marcadores Genéticos , Variação Genética , Genoma Fúngico/genética , Genótipo , Humanos , Dados de Sequência Molecular , Polimorfismo de Nucleotídeo Único , Análise de Sequência de DNARESUMO
Microsporidia are eukaryotic, intracellular obligate parasites that infect invertebrate and vertebrate animals, and have emerged as important opportunistic parasites in AIDS patients. We used light microscopy to detect microsporidial spores in stool samples of a domestic cat confirmed as Encephalitozoon intestinalis by PCR, owned by an AIDS patient with chronic diarrhea and E. intestinalis infection. Cats can be considered hosts of E. intestinalis.
Assuntos
Síndrome da Imunodeficiência Adquirida/complicações , Doenças do Gato/microbiologia , Encephalitozoon/classificação , Encefalitozoonose/veterinária , Albendazol/uso terapêutico , Animais , Anti-Helmínticos/uso terapêutico , Gatos , Encefalitozoonose/complicações , Encefalitozoonose/tratamento farmacológico , Encefalitozoonose/microbiologia , Humanos , Masculino , Pessoa de Meia-IdadeRESUMO
The work is described by microscopic analysis, the serological analysis (IFAT) and the molecular analysis of isolates from clinical samples (blood, faeces and urine) from ten domestic rabbits (Oryctolagus cuniculus), breed Malický, four New Zealand domestic rabbits, 11 sows of breed Slo0076akian Improved White and 15 clinically healthy laboratory BALB/c mice. The aim of the study was to validate the suitability of species-unspecific primer pairs 530F and 580R for genotype determination of the Microsporidia strain and species-specific primer pairs ECUNF and ECUNR, SINTF and SINTR and EBIER1 and EBIEF1 for the determination of E ncephalitozoon cuniculi, Encephalitozoon intestinalis and Enterocytozoon bieneusi species for diagnostic purposes. Sequences of animals were compared with those from the GenBank database. In rabbits, two murine genotypes II and four canine genotypes III were identified. Genotype II was identified in mice. The Encephalitozoon intestinalis identified in the sample from swine showed no genetic heterogeneity.
Assuntos
Encephalitozoon/classificação , Encephalitozoon/isolamento & purificação , Encefalitozoonose/veterinária , Técnicas de Diagnóstico Molecular/métodos , Reação em Cadeia da Polimerase/métodos , Medicina Veterinária/métodos , Animais , Primers do DNA/genética , Encephalitozoon/genética , Encefalitozoonose/diagnóstico , Camundongos , Camundongos Endogâmicos BALB C , Coelhos , SuínosRESUMO
We detected and identified genotypes of human-pathogenic microsporidia in fecal samples from 51 asymptomatic captive-bred pet parrots in South Korea. Microsporidia were identified in 8 samples (15.7%); 7 parrots tested positive for Encephalitozoon hellem, and 1 parrot tested positive for both E. hellem and Encephalitozoon cuniculi. In genotypic identifications, E. hellem was present in genotypes 1A and 2B and E. cuniculi was present in genotype II. Pet parrots might be a source of human microsporidian infection.
Assuntos
Doenças das Aves/diagnóstico , Doenças das Aves/microbiologia , Encephalitozoon/classificação , Encephalitozoon/isolamento & purificação , Encefalitozoonose/veterinária , Papagaios/microbiologia , Zoonoses/microbiologia , Animais , Doenças Assintomáticas , Doenças das Aves/transmissão , Análise por Conglomerados , DNA Fúngico/química , DNA Fúngico/genética , DNA Espaçador Ribossômico/química , DNA Espaçador Ribossômico/genética , Encephalitozoon/genética , Encefalitozoonose/diagnóstico , Encefalitozoonose/microbiologia , Encefalitozoonose/transmissão , Fezes/microbiologia , Genótipo , Humanos , Dados de Sequência Molecular , Animais de Estimação , Filogenia , República da Coreia , Medição de Risco , Análise de Sequência de DNA , Zoonoses/transmissãoRESUMO
Abstract. The species identification of Enterocytozoon bieneusi and Encephalitozoon intestinalis is only possible using transmission electron microscopy (TEM), mo lecular techniques and immunofluorescence antibody assays (IFA). In this study, 50 positive and 50 negative fecal specimens for microsporidial spores using the Weber modified trichrome (WMT) staining technique were examined using IFA-MAbs. Of the 100 specimens examined, the microsporidial spores identified by IFA-MAbs were Enterocytozoon Bieneusi 42 (75%) Encephalitozoon intestinalis 7 (12.5%) and mixed infections 7 (12.5%). The sensitivity and specificity of IFA-MAbs in detecting microsporidial spores were 98% and 86%, respectively. The agreement between the WMT staining technique and IFA-MAbs was statistically significant by Kappa statistics (K = 0.840; p < 0.001). E. bieneusi was the commonest Microsporidia species isolated from the studied population; the presence of microsporidial spores detected by IFA-MAbs should be confirmed by other methods.
Assuntos
Encephalitozoon/classificação , Enterocytozoon/isolamento & purificação , Microsporidiose/microbiologia , Anticorpos Monoclonais/química , Encephalitozoon/isolamento & purificação , Fezes/microbiologia , Imunofluorescência/métodos , Humanos , Malásia , Microscopia Eletrônica de Transmissão , Sensibilidade e Especificidade , Esporos Fúngicos , Coloração e Rotulagem/métodosRESUMO
In the present population-based study, we determined the prevalences of the most common human-pathogenic microsporidia, Encephalitozoon spp. and Enterocytozoon bieneusi, in asymptomatic healthy people living in the Czech Republic. A total of 382 males and females (ages, 1 to 84 years) living in the Czech Republic, of whom 265 were Czech nationals and 117 were foreign students, were included in a study testing for the presence of microsporidia by use of coprology and molecular methods. Single-species infections with Enterocytozoon bieneusi or an Encephalitozoon sp. were detected for 9 and 136 individuals, respectively. Moreover, coinfections were detected for 14 individuals. Four genotypes of 3 human-pathogenic Encephalitozoon spp. and 7 E. bieneusi genotypes, including 3 novel genotypes, were detected. Some of these were reported in humans for the first time. The highest prevalence was recorded for individuals older than 50 years and for loose, unformed stool samples. These findings clearly show that exposure to microsporidia is common among immunocompetent people and that microsporidiosis is not linked to any clinical manifestation in healthy populations.
Assuntos
Infecções Assintomáticas/epidemiologia , Encephalitozoon/isolamento & purificação , Encefalitozoonose/epidemiologia , Encefalitozoonose/microbiologia , Adulto , República Tcheca/epidemiologia , DNA Fúngico/química , DNA Fúngico/genética , Encephalitozoon/classificação , Feminino , Humanos , Masculino , Pessoa de Meia-Idade , Dados de Sequência Molecular , Prevalência , Análise de Sequência de DNARESUMO
We describe a new microsporidian species, Encephalitozoon romaleae n. sp., isolated from an invertebrate host, the grasshopper Romalea microptera, collected near Weeks Island, Louisiana, and Jacksonville, Florida. This microsporidian is characterized by specificity to the gastric caecae and midgut tissues of the host and a life cycle that is nearly identical to that of Encephalitozoon hellem and Encephalitozoon cuniculi. Mature spores are larger (3.97 x 1.95 microm) than those of other Encephalitozoon species. Polar filament coils number 7 to 8 in a single row. Analysis of the small subunit (SSU) rDNA shows that E. romaleae fits well into the Encephalitozoon group and is a sister taxon to E. hellem. This is the first Encephalitozoon species that has been shown to complete its life cycle in an invertebrate host.
Assuntos
Encephalitozoon/classificação , Gafanhotos/parasitologia , Animais , DNA Fúngico/química , DNA Ribossômico/química , Encephalitozoon/genética , Encephalitozoon/ultraestrutura , Microscopia Eletrônica de Transmissão , Microscopia de Contraste de Fase , FilogeniaRESUMO
Encephalitozoon species are the most common microsporidian pathogens of humans and domesticated animals. We recently discovered a new microsporidium, Encephalitozoon romaleae, infecting the eastern lubber grasshopper Romalea microptera. To understand its evolutionary relationships, we compared partial gene sequences of alpha- and beta-tubulin and methionine aminopeptidase 2 enzyme from this and related species. We also analyzed the rRNA internal transcribed spacer. Based on tubulin and MetAP-2 gene phylogenetic analysis, E. romaleae clustered with the Encephalitzoon group with strong bootstrap support (>99%). Within the Encephalitozoon clade, E. romaleae clustered with Encephalitozoon hellem for both the beta-tubulin and MetAP-2 phylogenies based on ML tree. The alpha-tubulin based ML tree, however, placed the new microsporidium closer to Encephalitozoon cuniculi. The rRNA internal transcribed spacer region of E. romaleae has 91% homology with E. hellem.
Assuntos
Encephalitozoon/classificação , Encephalitozoon/fisiologia , Encefalitozoonose/microbiologia , Gafanhotos/microbiologia , Filogenia , Aminopeptidases/genética , Animais , DNA Fúngico/genética , DNA Ribossômico/genética , DNA Espaçador Ribossômico/genética , Encephalitozoon/enzimologia , Encephalitozoon/genética , Humanos , Metaloendopeptidases/genética , Dados de Sequência Molecular , RNA Ribossômico/genética , Tubulina (Proteína)/genéticaRESUMO
Human-associated microsporidia were frequently observed in fecal samples of 331 feral pigeons in Amsterdam, The Netherlands, obtained during high- and low-breeding periods. Thirty-six of 331 samples (11%) contained the human pathogens Enterocytozoon bieneusi (n = 18), Encephalitozoon hellem (n = 11), Encephalitozoon cuniculi (n = 6), and Encephalitozoon intestinalis (n = 1); 5 samples contained other microsporidia. Pigeon feces can be an important source of human microsporidian infection.
Assuntos
Columbidae/microbiologia , Encephalitozoon/classificação , Encephalitozoon/isolamento & purificação , Enterocytozoon/classificação , Enterocytozoon/isolamento & purificação , Fezes/microbiologia , Animais , DNA Fúngico/química , DNA Fúngico/genética , Encephalitozoon/genética , Enterocytozoon/genética , Humanos , Dados de Sequência Molecular , Países Baixos , Análise de Sequência de DNARESUMO
BACKGROUND: Microsporidia are intracellular parasites that are highly-derived relatives of fungi. They have compacted genomes and, despite a high rate of sequence evolution, distantly related species can share high levels of gene order conservation. To date, only two species have been analysed in detail, and data from one of these largely consists of short genomic fragments. It is therefore difficult to determine how conservation has been maintained through microsporidian evolution, and impossible to identify whether certain regions are more prone to genomic stasis. PRINCIPAL FINDINGS: Here, we analyse three large fragments of the Enterocytozoon bieneusi genome (in total 429 kbp), a species of medical significance. A total of 296 ORFs were identified, annotated and their context compared with Encephalitozoon cuniculi and Antonospora locustae. Overall, a high degree of conservation was found between all three species, and interestingly the level of conservation was similar in all three pairwise comparisons, despite the fact that A. locustae is more distantly related to E. cuniculi and E. bieneusi than either are to each other. CONCLUSIONS/SIGNIFICANCE: Any two genes that are found together in any pair of genomes are more likely to be conserved in the third genome as well, suggesting that a core of genes tends to be conserved across the entire group. The mechanisms of rearrangments identified among microsporidian genomes were consistent with a very slow evolution of their architecture, as opposed to the very rapid sequence evolution reported for these parasites.