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1.
Ultrasound Obstet Gynecol ; 58(1): 92-98, 2021 07.
Artigo em Inglês | MEDLINE | ID: mdl-32304613

RESUMO

OBJECTIVE: Esophageal atresia and/or tracheoesophageal fistula (EA/TEF) remains one of the most frequently missed congenital anomalies prenatally. The aim of our study was to elucidate the sonographic manifestation of EA/TEF throughout pregnancy. METHODS: This was a retrospective study of data obtained from a tertiary center over a 12-year period. The prenatal ultrasound scans of fetuses with EA/TEF were assessed to determine the presence and timing of detection of three principal signs: small/absent stomach and worsening polyhydramnios, both of which were considered as 'suspected' EA/TEF, and esophageal pouch, which was considered as 'detected' EA/TEF. We assessed the yield of the early (14-16 weeks' gestation), routine mid-trimester (19-26 weeks) and third-trimester (≥ 27 weeks) anomaly scans in the prenatal diagnosis of EA/TEF. RESULTS: Seventy-five cases of EA/TEF with available ultrasound images were included in the study. A small/absent stomach was detected on the early anomaly scan in 3.6% of fetuses scanned, without a definitive diagnosis. On the mid-trimester scan, 19.4% of scanned cases were suspected and 4.3% were detected. On the third-trimester anomaly scan, 43.9% of scanned cases were suspected and 33.9% were detected. An additional case with an esophageal pouch was detected on magnetic resonance imaging (MRI) in the mid-trimester and a further two were detected on MRI in the third trimester. In total, 44.0% of cases of EA/TEF in our cohort were suspected, 33.3% were detected and 10.7% were suspected but, eventually, not detected prenatally. CONCLUSIONS: Prenatal diagnosis of EA/TEF on ultrasound is not feasible before the late second trimester. A small/absent stomach may be visualized as early as 15 weeks' gestation. Polyhydramnios does not develop before the mid-trimester. An esophageal pouch can be detected as early as 22 weeks on a targeted scan in suspected cases. The detection rates of all three signs increase with advancing pregnancy, peaking in the third trimester. The early and mid-trimester anomaly scans perform poorly as a screening and diagnostic test for EA/TEF. © 2020 International Society of Ultrasound in Obstetrics and Gynecology.


Assuntos
Atresia Esofágica/diagnóstico por imagem , Atresia Esofágica/embriologia , Feto/diagnóstico por imagem , Feto/embriologia , Fístula Traqueoesofágica/diagnóstico por imagem , Fístula Traqueoesofágica/embriologia , Ultrassonografia Pré-Natal/estatística & dados numéricos , Esôfago/anormalidades , Esôfago/diagnóstico por imagem , Esôfago/embriologia , Feminino , Desenvolvimento Fetal , Humanos , Estudos Longitudinais , Poli-Hidrâmnios/diagnóstico por imagem , Valor Preditivo dos Testes , Gravidez , Segundo Trimestre da Gravidez , Terceiro Trimestre da Gravidez , Estudos Retrospectivos , Estômago/anormalidades , Estômago/diagnóstico por imagem , Estômago/embriologia , Ultrassonografia Pré-Natal/métodos
2.
Cell Physiol Biochem ; 39(1): 385-94, 2016.
Artigo em Inglês | MEDLINE | ID: mdl-27372649

RESUMO

BACKGROUND/AIMS: Patients with esophageal atresia (EA) and tracheoesophageal fistula (TEF) often suffer chronic respiratory tract disease. We previously reported that primary lung maldevelopment caused by deficient branching of embryonal airways in experimental EA-TEF was induced by Adriamycin. In this study, we investigated the Vascular endothelial growth factor (VEGF) pathway in the developing lung in an EA-TEF rat model. We further analyzed the effect of recombinant VEGF treatment in vitro on branching morphogenesis of embryo lungs in experimental EA-TEF. METHODS: Pregnant rats received either Adriamycin or vehicle on E7, E8 and E9. Lungs were recovered at E15, E18 and E21. Expression of VEGF and receptors (Flk-1 and Flt-1) were assessed by quantitative PCR, immunohistochemistry and immunoblotting. E13 lungs were cultured for 72 hours with 50 ng/mL of recombinant rat VEGF in serum-free medium. The rates of increase in bud count and airway contour were evaluated. RESULTS: Our results showed a significant downregulation of VEGF during pseudoglandular and canalicular stages. In contrast, there were significantly higher levels of the Flt-1 receptor in the canalicular stage, which may represent a compensatory response to decreased VEGF. However, both variables returned to normal levels at the saccular stage. Exogenous VEGF treatment enhanced hypoplastic lung growth, evidenced by the increase in bud count and airway contour. CONCLUSIONS: A VEGF signaling defect possibly plays an important role in defective embryonic airway branching. Additionally, VEGF treatment may accelerate lung growth in EA-TEF lungs.


Assuntos
Atresia Esofágica/genética , Pulmão/metabolismo , Morfogênese/genética , Transdução de Sinais/genética , Fator A de Crescimento do Endotélio Vascular/genética , Animais , Western Blotting , Doxorrubicina , Atresia Esofágica/induzido quimicamente , Atresia Esofágica/embriologia , Feminino , Regulação da Expressão Gênica no Desenvolvimento/efeitos dos fármacos , Humanos , Imuno-Histoquímica , Pulmão/embriologia , Pulmão/patologia , Morfogênese/efeitos dos fármacos , Técnicas de Cultura de Órgãos , Gravidez , Ratos Wistar , Proteínas Recombinantes/metabolismo , Proteínas Recombinantes/farmacologia , Reação em Cadeia da Polimerase Via Transcriptase Reversa , Fatores de Tempo , Fístula Traqueoesofágica/induzido quimicamente , Fístula Traqueoesofágica/embriologia , Fístula Traqueoesofágica/genética , Fator A de Crescimento do Endotélio Vascular/metabolismo , Fator A de Crescimento do Endotélio Vascular/farmacologia , Receptor 1 de Fatores de Crescimento do Endotélio Vascular/genética , Receptor 1 de Fatores de Crescimento do Endotélio Vascular/metabolismo
3.
J Pediatr Surg ; 50(8): 1251-9, 2015 Aug.
Artigo em Inglês | MEDLINE | ID: mdl-26220889

RESUMO

INTRODUCTION: Esophageal atresia and tracheoesophageal fistula (EA-TEF) survivors suffer respiratory morbidity of unclear pathogenesis. Defective lung morphogenesis has been described in the rat model. This study examined fetal lung growth and maturity in rats and patients with EA-TEF. METHODS: Pregnant rats received either adriamycin or vehicle. Control and adriamycin-exposed lungs, with and without EA-TEF, were weighed and processed for RT-PCR, DNA quantification, immunofluorescence and immunoblot analysis of TTF1, VEGF, Sp-B, and α-sma. Twenty human lungs were also processed for immunofluorescence and Alcian-blue staining. RESULTS: Lungs from fetuses with EA-TEF (E21) showed decreased total DNA; FGF7 and TTF1 mRNA expressions were upregulated at E15 and E18, respectively. Protein expression and immunofluorescent distribution of maturity markers were similar. Lungs from stillborns with EA-TEF showed decreased epithelial expression of Sp-B and VEGF whereas those from newborns tended to have less Sp-B and more VEGF and mucous glands. DISCUSSION: The lungs of rats with EA-TEF were hypoplastic but achieved near-normal maturity. Stillborns with EA-TEF exhibited an apparently disturbed differentiation of the airway epithelium. Newborns with EA-TEF demonstrated subtle differences in the expression of differentiation markers, and increased number of mucous glands that could influence postnatal respiratory adaptation and explain some respiratory symptoms of EA-TEF survivors.


Assuntos
Atresia Esofágica/embriologia , Maturidade dos Órgãos Fetais , Pulmão/embriologia , Fístula Traqueoesofágica/embriologia , Animais , Biomarcadores/metabolismo , Doxorrubicina , Atresia Esofágica/induzido quimicamente , Atresia Esofágica/metabolismo , Feminino , Humanos , Pulmão/metabolismo , Gravidez , Ratos , Ratos Sprague-Dawley , Fístula Traqueoesofágica/induzido quimicamente , Fístula Traqueoesofágica/metabolismo
4.
Eur Radiol ; 25(2): 306-14, 2015 Feb.
Artigo em Inglês | MEDLINE | ID: mdl-25304819

RESUMO

OBJECTIVE: Present challenges are to improve the diagnosis rate of oesophageal atresia (OA) and evaluate as completely as possible a fetus affected by OA, specifically the type of OA and the length of the gap. Our aim was to evaluate the accuracy of fetal MR imaging (fMRI) for diagnosis of OA. METHODS: We reviewed fMRI performed because of sonographic suspicion of an OA. The signs reviewed included stomach size, "pouch sign", bowing of the trachea and visualization of the lower oesophageal lumen. The fetuses were assigned by consensus as having or not having EA, as well as having a tracheaoesophageal fistula (TOF). All findings were correlated with postnatal data. Sensitivity, specificity, positive predictive value (PPV) and negative predictive value (NPV) were calculated. RESULTS: Se, Sp, PPV and NPV of the technique were respectively 91%, 100%, 100% and 88%. The presence of the pouch sign yielded corresponding values of 82%, 100%, 100% and 78%. Mid-tracheal bowing was correlated positively with EA. The type of atresia was correctly evaluated in 90% of patients. CONCLUSION: fMRI is useful for the diagnosis of EA through the visualization of the oesophageal pouch or through associated signs such as tracheal bowing. Visualization of the lower oesophageal lumen seems to be a good sign of TEF. KEY POINTS: • Challenges are to improve the prenatal diagnosis of EA and associated malformations. • fMRI is able to diagnose EA through demonstration of the pouch sign. • Tracheal bowing is a promising indirect sign of EA. • Tracheoesophageal fistula can also be suspected thanks to fMRI. • Obstetrical US, fMRI and fetal CT are complementary for assessing associated malformations.


Assuntos
Atresia Esofágica/diagnóstico , Doenças Fetais/diagnóstico , Adulto , Atresia Esofágica/embriologia , Feminino , Idade Gestacional , Humanos , Imageamento por Ressonância Magnética/métodos , Gravidez , Diagnóstico Pré-Natal/métodos , Estudos Retrospectivos , Sensibilidade e Especificidade , Estômago/embriologia , Fístula Traqueoesofágica/diagnóstico , Fístula Traqueoesofágica/embriologia
5.
Early Hum Dev ; 90(12): 947-50, 2014 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-25448787

RESUMO

Oesophageal atresia with tracheo-oesophageal fistula is a relatively common congenital anomaly occurring in around 1:2500 births. The aetiology and embryology of the condition remain unclear, whilst associations with other significant anomalies are common. Studies in rodent models are contributing to our understanding of the condition. Advances in surgical care and neonatal management have improved survival considerably to around 90%. Long-gap and isolated oesophageal atresia present significant management challenges. Post-operative and long-term complications including oesophageal stricture, gastro-oesophageal reflux and respiratory compromise however remain relatively common and continue to pose a challenge for the ongoing management of patients.


Assuntos
Atresia Esofágica/cirurgia , Desenvolvimento Infantil , Atresia Esofágica/embriologia , Atresia Esofágica/etiologia , Guias como Assunto , Humanos , Recém-Nascido , Complicações Pós-Operatórias , Prognóstico , Fístula Traqueoesofágica/embriologia , Fístula Traqueoesofágica/etiologia , Fístula Traqueoesofágica/cirurgia
6.
Pediatr Surg Int ; 30(2): 151-7, 2014 Feb.
Artigo em Inglês | MEDLINE | ID: mdl-24363086

RESUMO

PURPOSE: Gastrointestinal malformations such as esophageal atresia with tracheoesophageal fistula (EA/TEF) and duodenal atresia (DA) have been reported in infants born to hyperthyroid mothers or with congenital hypothyroidism. The present study aimed to test whether maternal thyroid status during embryonic foregut division has any influence on the prevalence of EA/TEF and DA in an accepted rat model of these malformations. METHODS: Pregnant rats received either vehicle or 1.75 mg/kg i.p. adriamycin on gestational days 7, 8 and 9. Transient maternal hyper or hypothyroidism was induced by oral administration of levothyroxine (LT4, 50 µg/kg/day) or propylthiouracil (PTU, 2 mg/kg/day), respectively, on days 7 to 12 of gestation. Plasma cholesterol, total T3, free T4 and TSH were measured at gestational days 7, 12, and 21. At the end of gestation, the mothers were sacrificed and embryo-fetal mortality was recorded. Fetuses were dissected to determine the prevalence of esophageal and intestinal atresias. RESULTS: At gestational day 12, mothers treated with LT4 or PTU had hyper or hypothyroid status, respectively; plasma cholesterol levels were similar. In the adriamycin-exposed fetuses from hyperthyroid mothers, the embryonal resorption rate and the prevalence of both EA/TEF and DA were significantly higher than in the other groups; maternal hypothyroidism during the same period did not have significant effect on the prevalence of atresias. CONCLUSIONS: Maternal hyperthyroidism during the embryonic window corresponding to foregut cleavage increased the prevalence of both EA/TEF and duodenal atresia in fetal rats exposed to adriamycin. This suggests that maternal thyroid hormone status might be involved in the pathogenesis of foregut atresias and invites further research on this likely clinically relevant issue in humans.


Assuntos
Doenças do Sistema Digestório/induzido quimicamente , Doenças do Sistema Digestório/embriologia , Atresia Esofágica/embriologia , Atresia Esofágica/etiologia , Hipertireoidismo/complicações , Complicações na Gravidez , Efeitos Tardios da Exposição Pré-Natal , Animais , Modelos Animais de Doenças , Doxorrubicina , Obstrução Duodenal/induzido quimicamente , Obstrução Duodenal/complicações , Atresia Esofágica/induzido quimicamente , Feminino , Atresia Intestinal , Gravidez , Ratos , Ratos Sprague-Dawley , Fístula Traqueoesofágica/induzido quimicamente , Fístula Traqueoesofágica/embriologia
7.
Pediatr Surg Int ; 30(2): 143-9, 2014 Feb.
Artigo em Inglês | MEDLINE | ID: mdl-24356861

RESUMO

INTRODUCTION: Oesophageal atresia/tracheo-oesophageal atresia (OA/TOF) frequently arises with associated anomalies and has been clinically linked with 22q11 deletion syndromes, a group of conditions due to Tbx1 gene mutation which include Di George syndrome. Tbx1 and Tbx2 genes modulate pharyngeal and cardiac development, but are also expressed in the developing foregut and are known to interact with key signalling pathways described in oesophageal formation including bone morphogenic proteins. The adriamycin mouse model (AMM) reliably displays OA/TOF-like foregut malformations providing a powerful system for investigating the disturbances in gene regulation and morphology involved in tracheo-oesophageal malformations. We hypothesised that foregut abnormalities observed in the AMM are associated with altered Tbx1 and Tbx2 gene expression. METHODS: Time-mated CBA/Ca mice received intra-peritoneal injection of adriamycin (for treated) or saline (for controls) on embryonic days (E)7 and 8. Untreated Cd1 embryos were used to establish normal expression patterns. Embryos harvested on E9-E11 underwent whole-mount in situ hybridization with labelled RNA probes for Tbx1 and Tbx2. Optical projection tomography was used to visualise expression in whole embryos by 3D imaging. RESULTS: Tbx1 expression was visualised in a highly specific pattern in the proximal oesophageal endoderm in normal and control embryos. In the AMM, extensive ectopic expression of Tbx1 was detected in the dorsal foregut and adjacent to the TOF. The focally restricted oesophageal expression pattern persisted in the AMM, but was posteriorly displaced in relation to the tracheal bifurcation. Tbx2 was widely expressed in the ventral foregut mesoderm of controls, lacking specific endoderm localisation. In the AMM, altered Tbx2 expression in the foregut was only seen in severely affected embryos. CONCLUSION: Highly specific Tbx1 expression in the proximal oesophageal endoderm suggests that Tbx1 may be an important regulator of normal oesophageal development. Altered Tbx1 expression in dorsal foregut and adjacent to the TOF in the AMM suggests that Tbx1 gene disruption may contribute to the pathogenesis of tracheo-oesophageal malformations.


Assuntos
Atresia Esofágica/genética , Esôfago/anormalidades , Regulação da Expressão Gênica no Desenvolvimento/genética , Proteínas com Domínio T/genética , Fístula Traqueoesofágica/genética , Animais , Modelos Animais de Doenças , Doxorrubicina , Atresia Esofágica/induzido quimicamente , Atresia Esofágica/embriologia , Esôfago/embriologia , Feminino , Camundongos , Camundongos Endogâmicos CBA , Gravidez , Fístula Traqueoesofágica/induzido quimicamente , Fístula Traqueoesofágica/embriologia
8.
Dis Esophagus ; 26(4): 356-8, 2013.
Artigo em Inglês | MEDLINE | ID: mdl-23679023

RESUMO

Foregut separation involves dynamic changes in the activities of signaling pathways and transcription factors. Recent mouse genetic studies demonstrate that some of these pathways interact with each other to form a complex network, leading to a unique dorsal-ventral patterning in the early foregut. In this review, it is discussed how this unique dorsal-ventral patterning is set prior to the foregut separation and how disruption of this patterning affects the separation process. Roles of downstream targets of these pathways in regulating separation at cellular and molecular levels would be discussed further. Understanding the mechanism of normal separation process will provide insights into the pathobiology of a relatively common birth defect, esophageal atresia with/without tracheoesophageal fistula.


Assuntos
Atresia Esofágica/embriologia , Regulação da Expressão Gênica no Desenvolvimento , Fístula Traqueoesofágica/embriologia , Animais , Biomarcadores/metabolismo , Proteínas Morfogenéticas Ósseas/genética , Proteínas Morfogenéticas Ósseas/metabolismo , Atresia Esofágica/genética , Atresia Esofágica/metabolismo , Proteínas Hedgehog/genética , Proteínas Hedgehog/metabolismo , Humanos , Camundongos , Mutação , Fatores de Transcrição SOXB1/genética , Fatores de Transcrição SOXB1/metabolismo , Fístula Traqueoesofágica/genética , Fístula Traqueoesofágica/metabolismo , Via de Sinalização Wnt/fisiologia
9.
Pediatr Radiol ; 43(10): 1385-90, 2013 Oct.
Artigo em Inglês | MEDLINE | ID: mdl-23567909

RESUMO

Tracheal agenesis is a rare and essentially lethal anomaly with divergent prenatal imaging findings depending on the presence or absence of a tracheoesophageal fistula. All prenatally diagnosed cases of tracheal agenesis reported to date have not had a tracheoesophageal fistula and presented with thoracoabdominal findings similar to congenital high airway obstruction syndrome. We present the case of a 32-week gestation fetus with rapid onset of polyhydramnios and no persistent findings of congenital high airway obstruction syndrome that was ultimately diagnosed with tracheal agenesis plus tracheoesophageal fistula by fetal MRI. Additionally, we present the novel uses of intraoperative US during a staged ex utero intrapartum therapy delivery and postdelivery MRI, facilitated by proximity within the neonatal ICU, to confirm diagnosis and direct patient management while minimizing unnecessary investigations.


Assuntos
Constrição Patológica/embriologia , Constrição Patológica/patologia , Imageamento por Ressonância Magnética/métodos , Traqueia/anormalidades , Fístula Traqueoesofágica/embriologia , Fístula Traqueoesofágica/patologia , Ultrassonografia Pré-Natal/métodos , Constrição Patológica/cirurgia , Parto Obstétrico/métodos , Humanos , Recém-Nascido , Masculino , Traqueia/embriologia , Traqueia/patologia , Traqueia/cirurgia , Fístula Traqueoesofágica/cirurgia
10.
Birth Defects Res B Dev Reprod Toxicol ; 95(2): 175-83, 2012 Apr.
Artigo em Inglês | MEDLINE | ID: mdl-22311705

RESUMO

The notochord has important structural and signaling properties during vertebrate development with key roles in patterning surrounding tissues, including the foregut. The adriamycin mouse model is an established model of foregut anomalies where exposure of embryos in utero to the drug adriamycin leads to malformations including oesophageal atresia and tracheoesophageal fistula. In addition to foregut abnormalities, treatment also causes branching, displacement, and hypertrophy of the notochord. Here, we explore the hypothesis that the notochord may be a primary target of disruption leading to abnormal patterning of the foregut by examining notochord position and structure in early embryos following adriamycin exposure. Treated (n = 46) and control (n = 30) embryos were examined during the crucial period when the notochord normally delaminates away from the foregut endoderm (6-28 somite pairs). Transverse sections were derived from the anterior foregut and analyzed by confocal microscopy following immunodetection of extracellular matrix markers E-cadherin and Laminin. In adriamycin-treated embryos across all stages, the notochord was abnormally displaced ventrally with prolonged attachment to the foregut endoderm. While E-cadherin was normally detected in the foregut endoderm with no expression in the notochord of control embryos, treated embryos up to 24 somites showed ectopic notochordal expression indicating a change in characteristics of the tissue; specifically an increase in intracellular adhesiveness, which may be instrumental in structural changes, affecting mechanical and signaling properties. This is consistent with disruption of the notochord leading to altered signaling to the foregut causing abnormal patterning and congenital foregut malformations.


Assuntos
Doxorrubicina/toxicidade , Embrião de Mamíferos/embriologia , Notocorda/efeitos dos fármacos , Notocorda/embriologia , Anormalidades Induzidas por Medicamentos/embriologia , Animais , Caderinas , Modelos Animais de Doenças , Embrião de Mamíferos/anormalidades , Embrião de Mamíferos/efeitos dos fármacos , Atresia Esofágica/induzido quimicamente , Atresia Esofágica/embriologia , Atresia Esofágica/patologia , Imuno-Histoquímica/métodos , Laminina/metabolismo , Camundongos , Camundongos Endogâmicos CBA , Microscopia Confocal/métodos , Notocorda/anormalidades , Fístula Traqueoesofágica/induzido quimicamente , Fístula Traqueoesofágica/embriologia , Fístula Traqueoesofágica/patologia
12.
Pediatr Surg Int ; 26(4): 407-11, 2010 Apr.
Artigo em Inglês | MEDLINE | ID: mdl-20182749

RESUMO

PURPOSE: The Adriamycin mouse model (AMM) is a reproducible teratogenic model of esophageal atresia/tracheo-esophageal fistula (EA/TEF). Tbx4 is a member of the T-box family of transcription factor genes, which is reported to play a key role in separation of the respiratory tract and the esophagus. Up-regulation of Tbx4 is reported to cause TEF in the chick. Optical projection tomography (OPT) is a technique that allows three-dimensional (3D) imaging of gene expression in small tissue specimens in an anatomical context. The aim of this study was to investigate the temporo-spatial expression of Tbx4 during the critical period of separation of the trachea and esophagus in normal and Adriamycin treated embryos using OPT. MATERIALS AND METHODS: Time-mated CBA/Ca mice received intraperitoneal injections of Adriamycin (6 mg/kg) or saline on days 7 and 8 of gestation. Embryos were harvested on days 9-12, stained following whole mount in situ hybridization with labeled RNA probes to detect Tbx4 transcripts (n = 5 for each treatment/day of gestation). Immunolocalization with the endoderm marker Hnf3beta was used to visualize morphology. Embryos were scanned by OPT to obtain 3D representations of gene expression domains. Animal licence no. B100/4106. RESULTS: OPT elegantly revealed Tbx4 gene expression in both controls and in the disorganized pulmonary mesenchyme in the treated embryos. Although characteristic morphological abnormalities were observed in Adriamycin treated embryos, there was no significant difference in Tbx4 transcript distribution around lung primordia in comparison with control embryos. CONCLUSION: Although previously reported morphological abnormalities of notochord and esophagus were observed in AMM, Tbx4 gene expression was unaltered, suggesting that esophageal anomalies can occur in the presence of normal Tbx4 gene expression in this model.


Assuntos
Atresia Esofágica/genética , Regulação da Expressão Gênica no Desenvolvimento/genética , Proteínas com Domínio T/genética , Fístula Traqueoesofágica/genética , Animais , Antibióticos Antineoplásicos/administração & dosagem , Modelos Animais de Doenças , Doxorrubicina/administração & dosagem , Atresia Esofágica/induzido quimicamente , Atresia Esofágica/embriologia , Esôfago/embriologia , Feminino , Imageamento Tridimensional/métodos , Hibridização In Situ/métodos , Masculino , Mesoderma , Camundongos , Camundongos Endogâmicos CBA , Cloreto de Sódio/administração & dosagem , Tomografia Óptica/métodos , Traqueia/embriologia , Fístula Traqueoesofágica/induzido quimicamente , Fístula Traqueoesofágica/embriologia
13.
Semin Pediatr Surg ; 18(1): 2-11, 2009 Feb.
Artigo em Inglês | MEDLINE | ID: mdl-19103415

RESUMO

Esophageal atresia (OA) and tracheoesophageal fistula (TOF) are important human birth defects of unknown etiology. The embryogenesis of OA/TOF remains poorly understood, mirroring the lack of clarity of the mechanisms of normal tracheoesophageal development. The development of rat and mouse models of OA/TOF has allowed the parallel study of both normal and abnormal embryogenesis. Although controversies persist, the fundamental morphogenetic process appears to be a rearrangement of the proximal foregut into separate respiratory (ventral) and gastrointestinal (dorsal) tubes. This process depends on the precise temporal and spatial pattern of expression of a number of foregut patterning genes. Disturbance of this pattern disrupts foregut separation and underlies the development of tracheoesophageal malformations.


Assuntos
Atresia Esofágica/embriologia , Fístula Traqueoesofágica/embriologia , Animais , Modelos Animais de Doenças , Atresia Esofágica/genética , Feminino , Humanos , Camundongos , Gravidez , Ratos , Fístula Traqueoesofágica/genética
14.
Development ; 134(13): 2521-31, 2007 Jul.
Artigo em Inglês | MEDLINE | ID: mdl-17522155

RESUMO

Sox2 is expressed in developing foregut endoderm, with highest levels in the future esophagus and anterior stomach. By contrast, Nkx2.1 (Titf1) is expressed ventrally, in the future trachea. In humans, heterozygosity for SOX2 is associated with anopthalmia-esophageal-genital syndrome (OMIM 600992), a condition including esophageal atresia (EA) and tracheoesophageal fistula (TEF), in which the trachea and esophagus fail to separate. Mouse embryos heterozygous for the null allele, Sox2(EGFP), appear normal. However, further reductions in Sox2, using Sox2(LP) and Sox2(COND) hypomorphic alleles, result in multiple abnormalities. Approximately 60% of Sox2(EGFP/COND) embryos have EA with distal TEF in which Sox2 is undetectable by immunohistochemistry or western blot. The mutant esophagus morphologically resembles the trachea, with ectopic expression of Nkx2.1, a columnar, ciliated epithelium, and very few p63(+) basal cells. By contrast, the abnormal foregut of Nkx2.1-null embryos expresses elevated Sox2 and p63, suggesting reciprocal regulation of Sox2 and Nkx2.1 during early dorsal/ventral foregut patterning. Organ culture experiments further suggest that FGF signaling from the ventral mesenchyme regulates Sox2 expression in the endoderm. In the 40% Sox2(EGFP/COND) embryos in which Sox2 levels are approximately 18% of wild type there is no TEF. However, the esophagus is still abnormal, with luminal mucus-producing cells, fewer p63(+) cells, and ectopic expression of genes normally expressed in glandular stomach and intestine. In all hypomorphic embryos the forestomach has an abnormal phenotype, with reduced keratinization, ectopic mucus cells and columnar epithelium. These findings suggest that Sox2 plays a second role in establishing the boundary between the keratinized, squamous esophagus/forestomach and glandular hindstomach.


Assuntos
Padronização Corporal , Diferenciação Celular , Proteínas de Ligação a DNA/metabolismo , Sistema Digestório/embriologia , Sistema Digestório/metabolismo , Endoderma/citologia , Endoderma/metabolismo , Transativadores/metabolismo , Animais , Proteínas de Ligação a DNA/genética , Atresia Esofágica/embriologia , Atresia Esofágica/genética , Atresia Esofágica/metabolismo , Atresia Esofágica/patologia , Fator 10 de Crescimento de Fibroblastos/genética , Fator 10 de Crescimento de Fibroblastos/metabolismo , Regulação da Expressão Gênica no Desenvolvimento , Camundongos , Camundongos Endogâmicos C57BL , Mutação/genética , Proteínas Nucleares/deficiência , Proteínas Nucleares/genética , Proteínas Nucleares/metabolismo , Fenótipo , Fatores de Transcrição SOXB1 , Fator Nuclear 1 de Tireoide , Fatores de Tempo , Fístula Traqueoesofágica/embriologia , Fístula Traqueoesofágica/genética , Fístula Traqueoesofágica/metabolismo , Fístula Traqueoesofágica/patologia , Transativadores/genética , Fatores de Transcrição/deficiência , Fatores de Transcrição/genética , Fatores de Transcrição/metabolismo
15.
J Pediatr Surg ; 42(2): 375-80, 2007 Feb.
Artigo em Inglês | MEDLINE | ID: mdl-17270552

RESUMO

BACKGROUND/PURPOSE: Organogenesis relies on temperospatially coordinated signaling systems. The adriamycin rat model provided insights into the dysmorphogenesis of tracheoesophageal malformations. An adriamycin mouse model (AMM) would facilitate the investigation of their molecular pathogenesis. To transfer the knowledge gained from the rat, we describe a histological account of the critical period of organogenesis of these malformations in the AMM. METHOD: CBA/Ca mice were accurately time-mated (n = 18). Dams received intraperitoneal injections of adriamycin (6 mg/kg) (n = 12) or saline control (n = 6) on days 7 and 8. Fetuses were harvested on days 9, 9.5, 10, 11, 12, and 13, resin embedded, and 1-mum sections of the developing foregut were examined. RESULTS: Day 11 control fetuses showed normal separation of the respiratory primordium, with apoptotic bodies at the point of separation. A more caudal point of separation of the distal foregut without apoptotic bodies was found in 4 of 10 AMM fetuses. Day 13 AMM fetuses had dorsal or ventral outpouchings of the foregut, indicating which malformation they would develop. Abnormal branching of the notochord was seen from day 9.5 in AMM fetuses. This was not always associated with abnormal tracheoesophageal development. CONCLUSION: This study confirms that the abnormal observations made in the rat model apply to the mouse.


Assuntos
Sistema Digestório/embriologia , Embrião não Mamífero , Atresia Esofágica/embriologia , Notocorda/embriologia , Fístula Traqueoesofágica/embriologia , Animais , Sistema Digestório/efeitos dos fármacos , Modelos Animais de Doenças , Doxorrubicina , Atresia Esofágica/induzido quimicamente , Atresia Esofágica/patologia , Feminino , Camundongos , Notocorda/anormalidades , Notocorda/efeitos dos fármacos , Valores de Referência , Fatores de Tempo , Fístula Traqueoesofágica/induzido quimicamente , Fístula Traqueoesofágica/patologia
16.
Pediatr Surg Int ; 23(5): 469-72, 2007 May.
Artigo em Inglês | MEDLINE | ID: mdl-17205296

RESUMO

A spectrum of tracheo-oesophageal malformations is seen in humans: oesophageal atresia, tracheal agenesis and laryngotracheo-oesophageal clefts. They are thought to share a common but unknown aetiology. These birth defects are frequently associated with other VACTERL anomalies. The adriamycin rat model (ARM) has proved to be a valuable model of the VACTERL anomalies, illustrating the dysmorphogenesis of oesophageal atresia and tracheal agenesis. As organogenesis relies on temporaspatially co-ordinated signalling systems, the next step would be to study the molecular pathogenesis of tracheo-oesophageal malformations. However, the mouse is the foremost mammal studied by developmental biologists, offering an expanding wealth of knowledge and scientific research techniques with which to investigate these anomalies. A limited dose response analysis of the teratogenicity of adriamycin in the mouse has identified a dose and timing of injections that produced tracheo-oesophageal malformations and other VACTERL anomalies. A clear account of the types and variability of the tracheo-oesophageal malformations produced by this dose is essential in order to be able to plan and interpret any future investigations of early gestation fetuses. CBA/Ca mice were accurately time-mated (n = 10). Nine dams received intraperitoneal injections of adriamycin (6 mg/kg) and one control dam received saline injections, on days 7 and 8. Fetuses were harvested on day 18, near term. Tracheo-oesophageal malformations were examined by dissecting microscope and serial transverse sections. Results are reported in the standard teratological manner as mean percentage per litter (+/-SEM). The resorption rate of the adriamycin treated fetuses was 50.4%. There were 29 adriamycin treated fetuses for inspection. Tracheo-oesophageal malformations were found in 29.2% (+/-10.3), affecting five out of nine litters. Oesophageal atresia occurred in 15.6% (+/-8.1), laryngotracheo-oesophageal cleft in 10.4% (+/-7) and tracheal agenesis in 3.1% (+/-3.1). All of these malformations occurred with a tracheo-oesophageal fistula. Unlike the ARM, the AMM can produce fetuses with complete laryngotracheo-oesophageal cleft as well as oesophageal atresia or tracheal agenesis. Their occurrence was found to be reproducible but variable. These are important considerations when planning and interpreting experiments using this model.


Assuntos
Antibióticos Antineoplásicos/toxicidade , Doxorrubicina/toxicidade , Doenças do Esôfago/induzido quimicamente , Esôfago/anormalidades , Traqueia/anormalidades , Doenças da Traqueia/induzido quimicamente , Animais , Modelos Animais de Doenças , Atresia Esofágica/induzido quimicamente , Atresia Esofágica/embriologia , Doenças do Esôfago/embriologia , Esôfago/efeitos dos fármacos , Esôfago/embriologia , Feminino , Camundongos , Camundongos Endogâmicos CBA , Reprodutibilidade dos Testes , Cloreto de Sódio/administração & dosagem , Traqueia/efeitos dos fármacos , Traqueia/embriologia , Doenças da Traqueia/embriologia , Fístula Traqueoesofágica/induzido quimicamente , Fístula Traqueoesofágica/embriologia
17.
Eur J Pediatr Surg ; 16(4): 260-4, 2006 Aug.
Artigo em Inglês | MEDLINE | ID: mdl-16981091

RESUMO

The authors present a set of female diamnionic and dichorionic twins with different blood types and congenital oesophageal atresia (EA) in both. Surgical management was successful. It can be assumed that EA with tracheo-oesophageal fistula in twin B occurred during an early embryological stage whereas the isolated EA in twin A was the result of a later event. To our knowledge, this is the first published set of dizygotic twins with different types of EA.


Assuntos
Doenças em Gêmeos , Atresia Esofágica/embriologia , Fístula Traqueoesofágica/embriologia , Feminino , Humanos , Recém-Nascido , Gêmeos Dizigóticos
18.
Pediatr Surg Int ; 22(2): 154-7, 2006 Feb.
Artigo em Inglês | MEDLINE | ID: mdl-16315037

RESUMO

The organogenesis of esophageal atresia with tracheoesophageal fistula (EA/TEF) remains unknown. The fistula tract appears to develop from a non-branching trifurcation of the embryonic lung bud. The non-branching growth of the fistula differs from the other lung buds and suggests a deficiency in bone morphogenetic protein (BMP) signaling, since BMPs are critical to proper lung development and branching. With IRB approval, portions of newborn human proximal esophageal pouch and distal fistula samples were recovered at the time of surgical repair of EA/TEF. The tissues were processed for immunohistochemistry. Commercially available fetal tissues were used as controls. In control tissues, BMP ligands (BMP 2, 4, and 7) were all present in the esophagus but absent in the trachea. BMPRIA was absent in both tissues. BMPRIB was detected in trachea but not in esophagus and BMPRII was detected in esophagus but not in trachea. In the EA/TEF specimens, all BMP ligands were present in the proximal esophageal pouch but absent in the fistula tract. BMPRIA and BMPRIB were not detected in either tissue. However, BMPRII was found in both fistula tract and proximal pouch. The submucosa of the fistula appears to maintain a mixed (identical neither to lung, esophagus, or trachea) BMP signaling pattern, providing one mechanism which could potentially explain the esophageal dismotility and lack of lung branching seen in the fistula/distal esophagus.


Assuntos
Receptores de Proteínas Morfogenéticas Ósseas Tipo II/metabolismo , Receptores de Proteínas Morfogenéticas Ósseas Tipo I/metabolismo , Proteínas Morfogenéticas Ósseas/metabolismo , Atresia Esofágica/embriologia , Fístula Traqueoesofágica/embriologia , Estudos de Casos e Controles , Atresia Esofágica/patologia , Humanos , Imuno-Histoquímica , Recém-Nascido , Ligantes , Transdução de Sinais , Fístula Traqueoesofágica/patologia
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