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1.
NPJ Biofilms Microbiomes ; 10(1): 69, 2024 Aug 14.
Artigo em Inglês | MEDLINE | ID: mdl-39143108

RESUMO

The gut microbiota (GM) can regulate bone mass, but its association with incident fractures is unknown. We used Cox regression models to determine whether the GM composition is associated with incident fractures in the large FINRISK 2002 cohort (n = 7043, 1092 incident fracture cases, median follow-up time 18 years) with information on GM composition and functionality from shotgun metagenome sequencing. Higher alpha diversity was associated with decreased fracture risk (hazard ratio [HR] 0.92 per standard deviation increase in Shannon index, 95% confidence interval 0.87-0.96). For beta diversity, the first principal component was associated with fracture risk (Aitchison distance, HR 0.90, 0.85-0.96). In predefined phyla analyses, we observed that the relative abundance of Proteobacteria was associated with increased fracture risk (HR 1.14, 1.07-1.20), while the relative abundance of Tenericutes was associated with decreased fracture risk (HR 0.90, 0.85-0.96). Explorative sub-analyses within the Proteobacteria phylum showed that higher relative abundance of Gammaproteobacteria was associated with increased fracture risk. Functionality analyses showed that pathways related to amino acid metabolism and lipopolysaccharide biosynthesis associated with fracture risk. The relative abundance of Proteobacteria correlated with pathways for amino acid metabolism, while the relative abundance of Tenericutes correlated with pathways for butyrate synthesis. In conclusion, the overall GM composition was associated with incident fractures. The relative abundance of Proteobacteria, especially Gammaproteobacteria, was associated with increased fracture risk, while the relative abundance of Tenericutes was associated with decreased fracture risk. Functionality analyses demonstrated that pathways known to regulate bone health may underlie these associations.


Assuntos
Fraturas Ósseas , Microbioma Gastrointestinal , Humanos , Masculino , Feminino , Fraturas Ósseas/microbiologia , Fraturas Ósseas/epidemiologia , Fraturas Ósseas/etiologia , Pessoa de Meia-Idade , Finlândia/epidemiologia , Idoso , Bactérias/classificação , Bactérias/genética , Bactérias/isolamento & purificação , Metagenoma , Estudos de Coortes , Incidência , Metagenômica/métodos , Proteobactérias/genética , Proteobactérias/isolamento & purificação , Fatores de Risco , Adulto
2.
Diagn Microbiol Infect Dis ; 110(1): 116411, 2024 Sep.
Artigo em Inglês | MEDLINE | ID: mdl-39018934

RESUMO

One of the main barriers for the implementation of metagenomic sequencing in routine diagnosis of infectious diseases is the presence of host DNA. While several enrichment methods are likely to overcome this issue, their effectiveness for specimens such as bone in the case of chronic infections remains to be determined. We compared the relevance of two methods for bacterial DNA enrichment when compared to a reference protocol during pretreatment of bone samples from fracture-related infections before HTS by both Illumina Miseq and Oxford Nanopore Technology (ONT). The bacterial/human DNA ratio was higher for either protocols than the reference technique (p = 0.00012), without any significant difference between them. HTS sensitivity over culture ranged from 21.7 % to 85 %. The ability of the studied protocols to improve the bacterial/human DNA ratio depends on the sequencing technique employed. In this context, there is room for improvement in enhancing the sensitivity of HTS for diagnostic purpose.


Assuntos
DNA Bacteriano , Sequenciamento de Nucleotídeos em Larga Escala , Humanos , Sequenciamento de Nucleotídeos em Larga Escala/métodos , DNA Bacteriano/genética , Fraturas Ósseas/microbiologia , Sensibilidade e Especificidade , Metagenômica/métodos , Masculino , Bactérias/genética , Bactérias/isolamento & purificação , Bactérias/classificação , Feminino , Pessoa de Meia-Idade , Idoso , Adulto
4.
Injury ; 54 Suppl 6: 110898, 2023 Nov.
Artigo em Inglês | MEDLINE | ID: mdl-38143112

RESUMO

Postoperative bone infection is a severe complication in the treatment of fractures. The management of this pathology is challenging, but recent advances have been made to achieve standardization that can help diagnosis and decision-making. However, we are unaware of studies validating these models in Latin America. Therefore, this study aims to collect data from patients with fracture-related infections treated in different institutions in Latin America to create a registry that will assist in future clinical decision-making regarding the diagnostic process and the surgical and medical treatment of these patients.


Assuntos
Fraturas Ósseas , Infecções , Humanos , Fraturas Ósseas/complicações , Fraturas Ósseas/microbiologia , Fraturas Ósseas/cirurgia , América Latina/epidemiologia , Sistema de Registros , Infecções/etiologia , Infecções/terapia
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