[HPLC and UPLC-MS detection of 5-HMF from rabbit ncurolymph after treated with Cornus officinalis].

OBJECTIVE: To detect 5-HMF from rabbit ncurolymph after given different dosage of Cornus officinalis via intragastric administration by HPLC and UPLC-MS. METHODS: Rabbit ncurolymph was cramped out three days after given low, medium, and high dosage of Cornus officinalis. 5-HMF from rabbit ncurolymph was detected with HPLC and UPLC-MS, respectively. RESULTS: 5-HMF from rabbit ncurolymph was detected with HPLC method only in rabbits given high dose group. Meanwhile, 5-HMF could be detected with UPLC-MS method in rabbits given medium as well as low dose group. CONCLUSION: These results suggest that 5-HMF can cross the blood brain barrier (BBB) of rabbits and enter the rabbit ncurolymph.

A gas chromatographic--mass spectrometric study of profiles of volatile metabolites in hepatic encephalopathy.

Volatile organic substances present in blood plasma and cerebrospinal fluids of certain control groups of human subjects and cirrhotic patients some of whom were suffering from hepatic encephalopathy were quantitatively analysed and identified. A rapid, reproducible, direct injection capillary column gas chromatographic method was developed for the concentration and detection of such volatiles at mg/l and lower concentrations. Of at least forty volatiles detected, twenty-one were identified. The mean concentration of one of these, 3-methylbutanal, was found to be significantly elevated (p less than 0.01) in chronic encephalopathics (2.37 +/- 0.79 mg/l, n = 18), when compared to the controls (0.30 +/- 0.08 mg/l, n = 20). Furthermore, the concentration of this component increased with the clinically diagnosed severity of the encephalopathic state. The presence of 3-methylbutanal is related to leucine, a branched-chain amino acid linked with hepatic encephalopathy.