RESUMO
Ginsenoside compound K (CK) is an active metabolite of ginsenoside and has been shown to have ameliorative property in various diseases. However, the detailed in vivo metabolism of this compound has rarely been reported. In the present study, a method using liquid chromatography quadrupole time-of-flight tandem mass spectrometry together with multiple data processing techniques, including extracted ion chromatogram, multiple mass defect filter and MS/MS scanning, was developed to detect and characterize the metabolites of CK in rat urine and feces. After oral administration of CK at a dose of 50 mg/kg, urine and feces were collected for a period of time and subjected to a series of pretreatment. A total of 12 metabolites were tentatively or conclusively identified, comprising 11 phase I metabolites and a phase II metabolite. Metabolic pathways of CK has been proposed, including oxidation, deglycosylation, deglycosylation with sequential oxidation and dehydrogenation and deglycosylation with sequential glucuronidation. Relative quantitative analyses suggested that deglycosylation was the main metabolic pathway. The result could offer insights for better understanding of the mechanism of its pharmacological activities.
Assuntos
Cromatografia Líquida de Alta Pressão/métodos , Fezes/química , Ginsenosídeos , Espectrometria de Massas em Tandem/métodos , Animais , Ginsenosídeos/análise , Ginsenosídeos/metabolismo , Ginsenosídeos/urina , Masculino , Metabolômica , Ratos , Ratos Sprague-Dawley , Espectrometria de Massas por Ionização por ElectrosprayRESUMO
Pharmacokinetic and metabolism studies of 12-riboside-pseudoginsengenin DQ (RPDQ), a novel ginsenoside with an anti-cancer effect, were carried out, aiming at discussing the characteristics of the ginsenoside with glycosylation site at C-12. In the pharmacokinetic analysis, we developed and validated a method by UPLC-MS to quantify RPDQ in rat plasma. In the range of 5â»1000 ng/mL, the assay was linear (R² > 0.9966), with the LLOQ (lower limit of quantification) being 5 ng/mL. The LOD (limit of detection) was 1.5 ng/mL. The deviations of intra-day and inter-day, expressed as relative standard deviation (RSD), were ≤ 3.51% and ≤ 5.41% respectively. The accuracy, expressed as relative error (RE), was in the range â»8.82~3.47% and â»5.61~2.87%, respectively. The recoveries were in the range 85.66~92.90%. The method was then applied to a pharmacokinetic study in rats intragastrically administrated with 6, 12, and 24 mg/kg RPDQ. The results showed that RPDQ exhibited slow oral absorption (Tmax = 7.0 h, 7.5 h, and 7.0 h, respectively), low elimination (t1/2 = 12.59 h, 12.83 h, and 13.74 h, respectively) and poor absolute bioavailability (5.55, 5.15, and 6.08%, respectively). Moreover, the investigation of metabolites were carried out by UPLC-QTOF-MS. Thirteen metabolites of RPDQ were characterized from plasma, bile, urine, and feces of rats. Some metabolic pathways, including oxidation, acetylation, hydration, reduction, hydroxylation, glycine conjugation, sulfation, phosphorylation, glucuronidation, glutathione conjugation, and deglycosylation, were profiled. In general, both the rapid quantitative method and a good understanding of the characteristics of RPDQ in vivo were provided in this study.
Assuntos
Antineoplásicos/farmacocinética , Ginsenosídeos/farmacocinética , Animais , Antineoplásicos/sangue , Antineoplásicos/metabolismo , Antineoplásicos/urina , Bile/química , Disponibilidade Biológica , Líquidos Corporais/metabolismo , Linhagem Celular Tumoral , Cromatografia Líquida de Alta Pressão/métodos , Fezes/química , Ginsenosídeos/sangue , Ginsenosídeos/metabolismo , Ginsenosídeos/urina , Humanos , Limite de Detecção , Ratos Wistar , Reprodutibilidade dos Testes , Espectrometria de Massas em Tandem/métodosRESUMO
This study describes the characterization of oxygenated metabolites of ginsenoside Rg1 in rat urine and plasma. These in vivo metabolites were profiled by using UHPLC-QTOF MS-based method. On the basis of high-resolution MS/MS data, and comparison with chemically synthesized authentic compounds, nine oxygenated metabolites of Rg1 were characterized as vinaginsenosides 21 and 22 (M1 and M2), vinaginsenoside R15 (M3), 6-O-(ß-d-glucopyranosyl)-20-O-(ß-d-glucopyranosyl) 3ß, 6α, 12ß, 20(S)-tetrahydroxy-24ξ-hydroxydammar-25-ene (M4 and M5), floralginsenoside A (M7 and M8), floralginsenoside B (M9) and epoxyginsenoside Rg1 (M13), respectively. Among these metabolites, M4, M5 and M13 are new ginsenosides and others were detected as in vivo metabolites of Rg1 for the first time. In addition, a series of oxygenated metabolites of Rh1 and deglycosylated metabolite of Rg1, were observed and characterized by comparing with compounds synthesized by us, which revealed an association between C-20 configuration and the extent of oxidation metabolism. Appearance of all these metabolites in blood stream and urine after i.v. dosing and oral administration of Rg1 was further examined, which clearly showed that mono-oxygenated metabolites of Rg1 were major circulating metabolites at the early stage after dosing. Characterization of exact chemical structures of these circulating metabolites contribute greatly to our understanding of chemical exposure after consumption of ginseng products, and provide valuable information for explaining multiple bioactivities of ginseng products.
Assuntos
Ginsenosídeos/metabolismo , Oxigênio/química , Animais , Ginsenosídeos/sangue , Ginsenosídeos/urina , Masculino , Ratos , Ratos Sprague-DawleyRESUMO
RATIONALE: Neopanaxadiol (NPD) is one of the major ginsenosides in Panax ginseng C. A. Meyer (Araliaceae) that has been suggested to be a drug candidate against Alzheimer's disease. However, few data are available regarding its metabolism in rats. METHODS: In this study, a method of ultraperformance liquid chromatography/quadrupole-time-of-flight mass spectrometry (UPLC/QTOFMS) was developed to identify major metabolites of NPD in the stomach, intestine, urine and feces of rats, with the aim of determining the main metabolic pathways of NPD in rats after oral administration. RESULTS: UPLC/QTOFMS revealed two metabolites in the stomach of rats, one metabolite in the intestine and two metabolites in feces. One metabolite, named M2, was isolated and purified from rats feces, which was identified as (20S,22S)-dammar-22,25-epoxy-3ß,12ß,20-triol based on extensive NMR spectroscopy and mass spectrometry data. The main metabolites of NPD in rats were the products of epoxidation, dehydrogenation and hydroxylation. NPD was predominantly metabolized by 20,22-double-bond epoxidation and rearrangement to yield an expoxidation product (M2). CONCLUSIONS: Based on the profiles of the metabolites, possible metabolic pathways of NPD in rats were proposed for the first time. This study provides new and available information on the metabolism of NPD, which is indispensable for further research on metabolic pathways of dammarane ginsengenins in vivo.
Assuntos
Ginsenosídeos/análise , Ginsenosídeos/metabolismo , Animais , Cromatografia Líquida de Alta Pressão/métodos , Fezes/química , Mucosa Gástrica/metabolismo , Ginsenosídeos/urina , Mucosa Intestinal/metabolismo , Intestinos/química , Panax/química , Ratos , Ratos Sprague-Dawley , Espectrometria de Massas em Tandem/métodosRESUMO
Oxygenated metabolites have been suggested as the major circulating metabolites of ginsenosides. In the current study, 10 oxygenated metabolites of ginsenoside Rb1 in plasma and urine of rat following iv dose were characterized by comparison with chemically synthesized authentic compounds as quinquenoside L16 (M1 and M2), notoginsenoside A (M3), ginsenoside V (M4 and M7), epoxyginsenoside Rb1 (M5 and M9), notoginsenoside K (M6), and notoginsenoside C (M8 and M10), 9 of which were detected as in vivo metabolites for the first time. After oral administration of ginsenoside Rb1, M3, M4, and M7 were observed as major circulating metabolites and presented in the bloodstream of rat for 24 h. Characterization of the exact chemical structures of these circulating metabolites could contribute greatly to our understanding of chemical exposure of ginsenosides after consumption of ginseng products and provide valuable information for explaining multiple bioactivities of ginseng products.
Assuntos
Ginsenosídeos/sangue , Ginsenosídeos/urina , Panax/metabolismo , Extratos Vegetais/sangue , Extratos Vegetais/urina , Animais , Cromatografia Líquida de Alta Pressão , Ginsenosídeos/química , Masculino , Espectrometria de Massas , Estrutura Molecular , Panax/química , Panax/classificação , Extratos Vegetais/química , Ratos , Ratos Sprague-DawleyRESUMO
20(S)-Protopanaxatriol (PPT), one of the aglycones of ginsenosides, has been shown to exert cardioprotective effects against myocardial ischemic injury. However, studies on PPT metabolism have rarely been reported. This study is the first to investigate the in vivo metabolism of PPT following oral administration by ultra-performance liquid chromatography coupled with electrospray ionization quadrupole time-of-flight tandem mass spectrometry (UPLC-Q/TOF-MS) and nuclear magnetic resonance (NMR) spectroscopy. The structures of the metabolites were identified based on the characteristics of their MS data, MS(2) data, and chromatographic retention times. A total of 22 metabolites, including 17 phase I and 5 phase II metabolites, were found and tentatively identified by comparing their mass spectrometry profiles with those of PPT. Two new monooxygenation metabolites, (20S,24S)-epoxy-dammarane-3,6,12,25-tetraol and (20S,24R)-epoxy-dammarane-3,6,12,25-tetraol, were chemicallly synthesized and unambiguously characterized according to the NMR spectroscopic data. The metabolic pathways of PPT were proposed accordingly for the first time. Results revealed that oxidation of (1) double bonds at Δ((24,25)) to form 24,25-epoxides, followed by rearrangement to yield 20,24-oxide forms; and (2) vinyl-methyl at C-26/27 to form corresponding carboxylic acid were the predominant metabolic pathways. Phase II metabolic pathways were proven for the first time to consist of glucuronidation and cysteine conjugation. This study provides valuable and new information on the metabolism of PPT, which is indispensable for understanding the safety and efficacy of PPT, as well as its corresponding ginsenosides.
Assuntos
Sapogeninas/urina , Administração Oral , Animais , Cromatografia Líquida de Alta Pressão , Cromatografia por Troca Iônica , Cisteína/química , Compostos de Epóxi/química , Fezes , Ginsenosídeos/urina , Espectroscopia de Ressonância Magnética , Masculino , Estrutura Molecular , Oxigênio/química , Ratos , Ratos Sprague-Dawley , Sapogeninas/farmacocinética , Software , Espectrometria de Massas por Ionização por Electrospray , Espectrometria de Massas em TandemRESUMO
A rapid resolution liquid chromatography coupled with quadruple-time-of-flight mass spectrometry (RRLC-Q-TOF-MS) method was developed for pharmacokinetic study of ginsenoside Rc and applied in the simultaneous determination of ginsenoside Rc metabolites in rats. The experimental results indicate that the concentration versus time profile of ginsenoside Rc shows a two-compartment pharmacokinetic model after intravenous administration of ginsenoside Rc at a dosage of 0.4mg/kg for rats. In the metabolic study, prototype ginsenoside Rc and its deglycosylated metabolites Mb, Mc, and compound K were characterized by comparing the retention time (tR), accurate mass, and characteristic MS/MS fragment ions with standard compounds. The experiments show that part of the ginsenoside Rc was excreted through urine as prototype and part was metabolized into metabolites Mb and Mc after intravenous administration. In contrast, most of ginsenoside Rc were transformed into Mc and CK in feces after oral administration. The in vivo metabolic pathway of ginsenoside Rc was summarized.
Assuntos
Ginsenosídeos/farmacocinética , Administração Intravenosa , Administração Oral , Animais , Química Farmacêutica , Cromatografia Líquida , Fezes , Trato Gastrointestinal/efeitos dos fármacos , Ginsenosídeos/administração & dosagem , Ginsenosídeos/urina , Glicosilação , Masculino , Espectrometria de Massas , Controle de Qualidade , Ratos , Ratos Wistar , Espectrometria de Massas em Tandem , Fatores de Tempo , UrináliseRESUMO
Due to its effect of systems regulation and promotion on body, Ginseng is always referred to be long-term used as a dietary supplement. But it was still unclear about its target of the tonic effects and also the side-effects long-term use may bring. Urine metabolomic method is suitable for long-term studies of pharmaco-dynamics, pharmacology and toxicology of traditional Chinese medicine because of its characteristics of non-invasive and monitoring the whole-body metabolism. This study was designed to detect the dynamic variation of rat urine metabolome along with a long-term administration of total ginsenosides using GC-TOF based metabolomic technology. Our result showed that either short-term or chronic administration of ginsenosides did not impact the rat urine metabolome significantly (as the PCA subgroup was not successful). By comparison, the short-term (1-3 w) dose of ginsenosides had the biggest metabolic influence including TCA cycle, catecholamines and neurotransmitter amino acids. Medium-term (6-10 w) dose had a gradually lower effect and long-term (27 w) dose almost had no effect. Our study indicates that both short and long-term administration of ginsenosides showed almost no obvious side-effect on the experimental animals.
Assuntos
Medicamentos de Ervas Chinesas/metabolismo , Ginsenosídeos/urina , Panax/metabolismo , Animais , Ginsenosídeos/metabolismo , Masculino , Metabolômica , Ratos , Ratos Wistar , Fatores de TempoRESUMO
Following oral administration of ginsenoside Re, the compound and its metabolites were identified and quantified in rat urine and faeces by liquid chromatography coupled with triple quadrupole mass spectrometry (LC-MS/MS). Ginsenoside Re (200 mg/kg) was orally administered to rats by gastric intubation, and urine and faeces samples were then collected during the next 24 h using metabolic cages. Samples were prepared by solid phase extraction and analysed by LC-MS/MS. The precursor-product ion pairs used for LC-MS/MS analysis were: m/z 945â475 for ginsenoside Re, 799â637 for ginsenoside Rg1, 783â475 for ginsenoside Rg2, 637â475 for ginsenosides Rh1 and F1, 475â391 for protopanaxatriol, and 779â641 for digoxin (internal standard). The major ginsenosides excreted in urine were ginsenosides Re and Rg1, and only minimal amounts of ginsenosides Rg2 and Rh1 were found. Greater amounts of ginsenoside metabolites were detected in the faeces samples; biotransformation to ginsenoside Rg1 was predominant but further deglycosylated metabolites including ginsenoside F1 and protopanaxatriol were additionally detected. The total recovery of ginsenosides over 24 h was approximately 46%.
Assuntos
Fezes/química , Ginsenosídeos/metabolismo , Urina/química , Administração Oral , Animais , Biotransformação , Ginsenosídeos/administração & dosagem , Ginsenosídeos/urina , Masculino , RatosRESUMO
Ginsenoside Re (G-Re) improved the memory function of experimental animals in a preclinical study. Several types of saponins including G-Rg1, G-Rg2, G-F1, G-Rh1, and protopanaxatriol (PPT) may be the metabolites of G-Re according to reports from preclinical trials. In order to support a study of the pharmacokinetics of G-Re, an analytical method for G-Re and the co-detection of its probable metabolites using liquid chromatography tandem mass spectrometry (LC-MS/MS) was developed and validated. Solid phase extraction was utilized in the sample preparation. Separation of the analytes was achieved using a gradient elution (0.05% formic acid-methanol-acetonitrile, each organic phase containing 0.05% formic acid) at a flow rate of 0.3 mL/min with a retention time of approximately 2.88 min for G-Re. Data were acquired in the multiple reaction mode (MRM) and the linear range of the standard curve of plasma and urine samples for G-Re was 0.05-20 ng/mL with r(2)≥0.99. In the analysis of probable metabolites, G-Re, G-Rg1, G-F1, G-Rh1 and PPT were all detected in samples; however, G-Rg2 was not detected.
Assuntos
Cromatografia Líquida/métodos , Ginsenosídeos/sangue , Ginsenosídeos/urina , Sapogeninas/sangue , Sapogeninas/urina , Espectrometria de Massas em Tandem/métodos , Estabilidade de Medicamentos , Ginsenosídeos/metabolismo , Ginsenosídeos/farmacocinética , Humanos , Modelos Lineares , Reprodutibilidade dos Testes , Sapogeninas/metabolismo , Sapogeninas/farmacocinética , Sensibilidade e Especificidade , Extração em Fase SólidaRESUMO
OBJECTIVE: To develop and validate a HPLC-UV-ELSD method for the simultaneous determination of ginsenosides and epimedium flavonoids in rat urine after intravenous administration of Jiweiling freeze-dried powder. METHOD: Chromatographic separation was performed on a C18 HPLC column, with gradient elution of acetonitrile and water as mobile phase. An UV detector was used at detection wavelength of 220 nm. An evaporative light scattering detector (ELSD) was used at drift tube temperature of 80 degrees C and gas pressure of 172.4 kPa. RESULT: The calibration curves were linear over the investigated concentration ranges with all correlation coefficients higher than 0.998. The a intra- and inter-day RSD were less than 9.1% and the relative errors were verage extraction recoveries for all compounds were between 88.67% and 101.2%. The within the range of -11.58% to 10.89%. CONCLUSION: The proposed method showed appropriate accuracy and selectivity and was successfully applied to the rat urine samples analysis of saponins and flavonoids after intravenous administration of Jiweiling freeze-dried powder, which may provide some references to the apprehension of the action mechanism and clinical application.
Assuntos
Flavonoides/urina , Ginsenosídeos/urina , Raios Ultravioleta , Animais , Calibragem , Cromatografia Líquida de Alta Pressão/métodos , Epimedium/química , Epimedium/metabolismo , Fitoterapia , Preparações de Plantas , Ratos , Saponinas/análiseRESUMO
Here we report a method capable of quantifying ginsenoside Rg3 in human plasma and urine. The method was validated over linear range of 2.5-1000.0ngmL(-1) for plasma and 2.0-20.0ngmL(-1) for urine using ginsenoside Rg1 as I.S. Compounds were extracted with ethyl acetate and analyzed by HPLC/MS/MS (API-4000 system equipped with ESI(-) interface and a C(18) column). The inter- and intra-day precision and accuracy of QC samples were Assuntos
Cromatografia Líquida de Alta Pressão/métodos
, Ginsenosídeos
, Espectrometria de Massas em Tandem/métodos
, Estabilidade de Medicamentos
, Ginsenosídeos/sangue
, Ginsenosídeos/química
, Ginsenosídeos/farmacocinética
, Ginsenosídeos/urina
, Humanos
, Modelos Lineares
, Sensibilidade e Especificidade
RESUMO
The development of liquid chromatography-mass spectrometry (LC-MS) and tandem MS/MS for the analysis of bioactive components and their metabolites of herbal medicines in biological fluids is reviewed with the aim of providing an overview of the current techniques and methods used. The issues and challenges associated with various stages of the analytical method development are discussed using Ginkgo biloba and Panax ginseng as case studies. LC-MS offers selectivity and specificity in both the chromatographic separation and detection steps. This is necessary in order to measure compounds at extremely low concentrations as is often observed in plasma and urine samples. Traditional methods of detection (UV-visible) do not offer sufficient selectivity and specificity needed. The strategies and pitfalls involved with the measurement of such compounds are discussed in this review. Matrix effects, 'unseen' matrix suppression and enhancement ionization effects can significantly reduce the accuracy and precision of the measurement. The impact of the correct choice of chromatography column formats on signal-to-noise ratio is also discussed. Analytical methods from sample preparation to mass spectrometric detection is outlined in order to provide good direction for analysts intent on the measurement of bioavailable compounds from herbal medicines in plasma and urine samples.
Assuntos
Cromatografia Líquida/métodos , Ginkgo biloba/química , Ginsenosídeos/sangue , Ginsenosídeos/urina , Panax/química , Espectrometria de Massas em Tandem/métodos , Líquidos Corporais/química , Flavonoides/sangue , Flavonoides/urina , Humanos , Plantas Medicinais/químicaRESUMO
Ginsenoside Re is one of the major the bioactive triterpene saponins in ginseng root, a well-known adaptogen in traditional Chinese medicine. It is believed that the lead compound may be further developed into a promising new drug for preventing hypertension and cardiovascular disease. To better understand the pharmacological activities of the component, an investigation of its in vivo metabolism was necessary. In the present study, a high-performance liquid chromatography coupled with electrospray ionization and quadrupole time-of-flight tandem mass spectrometry (HPLC-ESI-TOF-MS/MS) has been applied to discover and identify the metabolites of ginsenoside Re in rat urine following intravenous and oral administration of the component, respectively. The rat urine samples were collected and pretreated through C(18) solid-phase extraction cartridges prior to analysis. Negative electrospray ionization mass spectrometry was used to discern ginsenoside Re and its possible metabolites in urine samples. The metabolites were identified and tentatively characterized by means of comparing molecular mass, retention time, and fragmentation pattern of the analytes with those of the parent compound, ginsenoside Re. As a result, eleven and nine metabolites together with Re were detected and identified in rat urine collected after intravenous and oral administration, respectively. A possible metabolic pathway of ginsenoside Re was also investigated and proposed. Oxidation and deglycosylation were found to be the major metabolic processes of the constituent in rat.
Assuntos
Cromatografia Líquida de Alta Pressão/métodos , Ginsenosídeos/urina , Espectrometria de Massas por Ionização por Electrospray/métodos , Espectrometria de Massas em Tandem/métodos , Administração Oral , Animais , Ginsenosídeos/administração & dosagem , Ginsenosídeos/química , Glicosilação , Injeções Intravenosas , Masculino , Estrutura Molecular , Oxirredução , Ratos , Ratos Sprague-DawleyRESUMO
OBJECTIVE: To determine the ginsenoside Rg2 and study its excretion in bile, feces and urine of rat. METHOD: Reversed phase high-performance liquid chromatographic (RP-HPLC) method with an ultra-violet detector (UVD) was performed at a detection wavelength of 203 nm and with a Dikma Diamonsil C18 column (4.6 mm x 250 mm, 5 microm), which the mobile phase was consisted of methanol-aq. 4% H3PO4 (65:35), for determination of the ginsenoside Rg2 in bile, feces and urine after administration of the ginsenoside Rg2 to rat at a tail vein single dose of 20 mg x kg(-1). RESULT: The HPLC-UVD method fulfilled all the standard requirements of linearity, recovery, precision, and accuracy. After tail vein administration of the ginsenoside Rg2 to rat, the 5.5 hour cumulative biliary excretion rate and the 24-hour cumulative feces excretion rate of intact ginsenoside Rg2 were 27.2% and 22.6% of the administered dose, respectively. But intact ginsenoside Rg2 could not be detected in urine during this experimental period. CONCLUSION: The bile and feces were the main excretion routs of the unchanged form after tail vein administration of the ginsenoside Rg2 to rat.
Assuntos
Medicamentos de Ervas Chinesas/química , Fezes/química , Ginsenosídeos/urina , Animais , Química Farmacêutica , Cromatografia Líquida de Alta Pressão , Ginsenosídeos/análise , Ratos , Ratos Wistar , Tecnologia FarmacêuticaRESUMO
20(S)-Ginsenoside Rh1 is one of the important protopanaxatriol ginsenosides and has been reported to be the main hydrolysis product reaching the systemic circulation after oral ingestion of ginseng. However, its pharmacokinetic characteristics and metabolic fate have never been reported. The present study was therefore designed to elucidate its pharmacokinetic profiles and metabolic pathways both in vivo and in vitro. The absolute bioavailability of 20(S)-ginsenoside Rh1 in rats was only 1.01 %. Identification of metabolites showed that, after intragastrical administration of ginsenoside Rh1, two mono-oxygenated metabolites were detected from the urine, bile, liver tissue, and intestinal tract content, while the de-glucosylated product, 20(S)-protopanaxatriol, was only found in the contents of the intestinal tract. An in vitro incubation study confirmed that the CYP450-catalyzed mono-oxygenation, the intestinal bacteria mediated de-glucosylation, and the gastric acid mediated hydration reaction were the main metabolic pathways of 20(S)-ginsenoside Rh1. The presystemic metabolism as evidenced from this study may partially explain its poor bioavailability.
Assuntos
Ginsenosídeos/metabolismo , Ginsenosídeos/farmacocinética , Panax/química , Extratos Vegetais/farmacocinética , Animais , Bile/metabolismo , Disponibilidade Biológica , Sistema Enzimático do Citocromo P-450/metabolismo , Ácido Gástrico/metabolismo , Ginsenosídeos/urina , Mucosa Intestinal/metabolismo , Fígado/metabolismo , Masculino , Redes e Vias Metabólicas , Extratos Vegetais/metabolismo , Extratos Vegetais/urina , Ratos , Ratos Sprague-DawleyRESUMO
OBJECTIVE: To determine the pseudo-ginsenoside GQ (PGQ) in rat bile, feces and urine, and to study on the excretion of pseudo-ginsenoside GQ. METHOD: Reverse phase high-performance liquid chromatography (RP-HPLC) method with an evaporative light-scattering detector (ELSD) was performed on Diamonsil C18 column (4.6 mm x 250 mm, 5 microm), and the mobile phase was consisted of methanol-water (24: 7) with flow rate of 1.0 mL x min(-1). ELSD parameters were set as follows: nitrogen gas pressure 3.0 bar, drift tube temperature 50 degrees C. RESULT: The method fulfilled all the standard requirements of precision, accuracy and linearity. The main way of excretion of PGQ in rat administrated through sublingual vein was at the bile. The bile excretion ratio of PGQ was 41.60%, and feces excretion ratio was 9.97%. Only trace amount of PGQ was excreted in urine. CONCLUSION: Almost all unchanged PGQ was excreted in bile, feces and urine.
Assuntos
Ginsenosídeos/farmacocinética , Ginsenosídeos/urina , Animais , Bile/metabolismo , Cromatografia Líquida de Alta Pressão , Fezes , Feminino , Ginsenosídeos/administração & dosagem , Ginsenosídeos/metabolismo , Masculino , Ratos , Ratos Sprague-Dawley , Ratos Wistar , Reação em Cadeia da Polimerase Via Transcriptase ReversaRESUMO
The goal of this study was the application of a novel, fully automatic column-switching approach in a metabonomics study combining the orthogonal selectivities of hydrophilic interaction chromatography (HILIC) and reversed-phase chromatography. The temporal, pharmacodynamic effects of the ginsenoside Rg3 on the metabonome in urine of healthy and liver-tumor-bearing rats have been investigated. Within a total analysis time of 52 min we detected 5686 polar, and on the second column an additional 1808 apolar, urinary metabolite ions. The administration of a single, high dose of Rg3 in a beta-cyclodextrin-based formulation led to a considerable change of the metabolic pattern in cancer rats during 3 days studied. Seventeen biomarker candidates including three apolar metabolites, which were not retained on the HILIC column, were detected. Overall, the results suggest that the developed liquid chromatography-mass spectrometry strategy is a promising tool in metabonomics studies for global analysis of highly complex biosamples. It may not only increase the number of discovered biomarkers but consequently improve the comprehensive information on metabolic changes in a fully automatic manner.
Assuntos
Cromatografia Líquida de Alta Pressão/métodos , Ginsenosídeos/urina , Glicômica/métodos , Interações Hidrofóbicas e Hidrofílicas , Neoplasias/metabolismo , Espectrometria de Massas por Ionização por Electrospray/métodos , beta-Ciclodextrinas/urina , Animais , Biomarcadores , Linhagem Celular Tumoral , Saúde , Masculino , Estrutura Molecular , Ratos , Ratos Sprague-DawleyRESUMO
Ginsenoside Rb1 is an active protopanaxadiol saponin from Panax species. In order to compare the similarities and differences of microbial and mammalian metabolisms of ginsenoside Rb1, the microbial transformation by Acremonium strictum and metabolism in rats were comparatively studied. Microbial transformation of ginsenoside Rb1 by Acremonium strictum AS 3.2058 resulted in the formation of eight metabolites. Ten metabolites (M1-M10) were detected from the in vivo study in rats and eight of them were identified as the same compounds as those obtained from microbial metabolism by liquid chromatography-tandem mass spectrometry analysis and comparison with reference standards obtained from microbial metabolism. Their structures were identified as ginsenoside Rd, gypenoside XVII, 20(S)-ginsenoside Rg3, 20(R)-ginsenoside Rg3, ginsenoside F2, compound K, 12beta-hydroxydammar-3-one-20(S)-O-beta-d-glucopyranoside, and 25-hydroxyl-(E)-20(22)-ene-ginsenoside Rg3, respectively. The structures of the additional two metabolites were tentatively characterized as 20(22),24-diene-ginsenoside Rg3 and 25-hydroxyginsenoside Rd by HPLC-MS/MS analysis. M7-M10 are the first four reported metabolites in vivo. The time course of rat metabolism of ginsenoside Rb1 was also investigated.
Assuntos
Acremonium/metabolismo , Cromatografia Líquida de Alta Pressão/métodos , Ginsenosídeos/metabolismo , Espectrometria de Massas em Tandem/métodos , Animais , Fezes/química , Ginsenosídeos/química , Ginsenosídeos/urina , RatosRESUMO
A rapid analytical method for quantifying 17 ginsenosides in rat urine by ultra performance liquid chromatography (UPLC) coupled to electrospray ionization mass spectrometry (ESI-MS) is described. All analytes were extracted by solid-phase extraction optimized to obtain good recovery and quantified using digoxin as an internal standard. ESI-MS was optimized for different cone voltages at positive ionization mode to allow simultaneous analysis of all analytes in a relatively short time. Qualitative methodological considerations, including the linear range, precision, limit of quantification, limit of detection, recovery and sensitivity are also provided.