RESUMO
CD8 virtual memory T (TVM) cells are Ag-naive CD8 T cells that have undergone partial differentiation in response to common γ-chain cytokines, particularly IL-15 and IL-4. TVM cells from young individuals are highly proliferative in response to TCR and cytokine stimulation but, with age, they lose TCR-mediated proliferative capacity and exhibit hallmarks of senescence. Helminth infection can drive an increase in TVM cells, which is associated with improved pathogen clearance during subsequent infectious challenge in young mice. Given the cytokine-dependent profile of TVM cells and their age-associated dysfunction, we traced proliferative and functional changes in TVM cells, compared with true naive CD8 T cells, after helminth infection of young and aged C57BL/6 mice. We show that IL-15 is essential for the helminth-induced increase in TVM cells, which is driven only by proliferation of existing TVM cells, with negligible contribution from true naive cell differentiation. Additionally, TVM cells showed the greatest proliferation in response to helminth infection and IL-15 compared with other CD8 T cells. Furthermore, TVM cells from aged mice did not undergo expansion after helminth infection due to both TVM cell-intrinsic and -extrinsic changes associated with aging.
Assuntos
Helmintíase , Interleucina-15 , Animais , Camundongos , Envelhecimento/imunologia , Linfócitos T CD8-Positivos/parasitologia , Citocinas , Helmintíase/imunologia , Helmintíase/metabolismo , Helmintos/patogenicidade , Memória Imunológica , Interleucina-15/metabolismo , Camundongos Endogâmicos C57BL , Receptores de Antígenos de Linfócitos TRESUMO
BACKGROUND: Rwanda is a sub-Saharan country, where intestinal parasite infections, anemia and undernutrition coexist. The purpose of this research is to study the relationship between intestinal parasite infections and undernutrition/anemia to clarify the priorities of intervention in the rural area of Gakenke district in the Northern Province of Rwanda. MATERIALS AND METHODS: A total of 674 students from Nemba I School, participated in a cross-sectional study, in which their parasitological and nutritional status were analysed. Statistical analysis was performed by χ2 test, univariate analysis and Odds ratios (OR). RESULTS: A total of 95.3% of children presented intestinal parasitism, most of whom (94.5%) infected by protozoa and 36.1% infected by soil-transmitted helminths (STH), with Trichuris trichiura (27.3%) being the most prevalent. Multiple infections were found to be high (83.8%), with protozoa and STH co-infections in 30.6%. STH infections were mainly of low/moderate intensity. Neither infection nor STH infection of any intensity profile, was significantly related to anemia. In addition, STH infection, regardless of the intensity profile, was not associated with stunting, underweight or thinness. There was no difference between genders nor among ages in odds of anemia and nutritional status in STH-infected schoolchildren. CONCLUSION: Multiparasitism remains high among Rwandan schoolchildren and is likely to cause nutritional problems. This work emphasizes the importance of keeping up health programs to reduce the prevalence of infection.
Assuntos
Anemia/epidemiologia , Desnutrição/epidemiologia , Adolescente , Animais , Criança , Pré-Escolar , Estudos Transversais , Fezes/parasitologia , Feminino , Transtornos do Crescimento/epidemiologia , Transtornos do Crescimento/parasitologia , Helmintíase/epidemiologia , Helmintos/patogenicidade , Humanos , Enteropatias Parasitárias/epidemiologia , Enteropatias Parasitárias/parasitologia , Masculino , Estado Nutricional/fisiologia , Prevalência , Ruanda/epidemiologia , Solo/parasitologia , Magreza/epidemiologia , Magreza/parasitologia , Tricuríase/epidemiologia , Trichuris/patogenicidadeAssuntos
Vacinas contra COVID-19/administração & dosagem , COVID-19/prevenção & controle , Ensaios Clínicos como Assunto , Helmintíase/imunologia , Helmintos/imunologia , Projetos de Pesquisa , SARS-CoV-2/imunologia , Desenvolvimento de Vacinas , África/epidemiologia , Animais , COVID-19/epidemiologia , COVID-19/imunologia , COVID-19/virologia , Vacinas contra COVID-19/efeitos adversos , Helmintíase/epidemiologia , Helmintíase/parasitologia , Helmintos/patogenicidade , Interações Hospedeiro-Patógeno , Humanos , Esquemas de Imunização , SARS-CoV-2/patogenicidade , Resultado do Tratamento , VacinaçãoRESUMO
Maternal infection during pregnancy is known to alter the development and function of offspring's immune system, leading to inappropriate immune responses to common childhood infections and immunizations. Although this is an expanding field, maternal parasitic infections remain understudied. Millions of women of reproductive age are currently at risk for parasitic infection, whereas many pregnant, chronically infected women are excluded from mass drug administration due partially to a lack of resources, as well as fear of unknown adverse fetal developmental outcomes. In areas endemic for multiple parasitic infections, such as sub-Saharan Africa, there are increased rates of morbidity and mortality for various infections during early childhood in comparison with nonendemic areas. Despite evidence supporting similar immunomodulatory effects between various parasite species, there is no clear mechanistic understanding of how maternal infection reprograms offspring immunity. This brief review will compare the effects of selected maternal parasitic infections on offspring immunity.
Assuntos
Desenvolvimento Fetal/imunologia , Helmintíase/imunologia , Malária Falciparum/imunologia , Doenças Parasitárias/transmissão , Complicações Parasitárias na Gravidez/epidemiologia , Adulto , África Subsaariana/epidemiologia , Animais , Feminino , Helmintíase/parasitologia , Helmintíase/transmissão , Helmintos/patogenicidade , Humanos , Recém-Nascido , Transmissão Vertical de Doenças Infecciosas , Malária Falciparum/parasitologia , Malária Falciparum/transmissão , Doenças Parasitárias/epidemiologia , Doenças Parasitárias/imunologia , Gravidez , Solo/parasitologiaRESUMO
Nectonema, the only horsehair worm (Nematomorpha) genus found in marine environments, was previously known to be parasitic only in decapod crustaceans. We report Nectonema sp. as the first record of a marine nematomorph parasitic in isopod crustaceans. This is also the third record of marine nematomorphs from the North Pacific. Six infected isopods (Natatolana japonensis) collected from 1425 m of depth in the Sea of Japan each contained one to seven (mean 2.33) nematomorphs in the body cavity in the pereon. There was no correlation between the host body length and number of parasites. For Nectonema sp., we describe and illustrate morphological features of the parasitic juvenile stage and present nucleotide sequences for the cytochrome c oxidase subunit I gene (COI or cox1; 451 nt), 18S rRNA gene (1777 nt), and region spanning the internal transcribed spacer 1 (ITS1) and the 28S rRNA gene including the 5.8S rRNA gene and ITS2 (1218 nt in total). In an 18S maximum-likelihood tree that included 24 nematomorph species, Nectonema sp. grouped with N. agile from the northwestern Atlantic; the 18S gene from these two taxa was divergent by 11.8% K2P distance, suggesting that they are different species. Nectonema species may have a broader range of host groups than previously suspected, but may have been previously misidentified as nematode parasites.
Assuntos
Helmintos/patogenicidade , Isópodes/parasitologia , Animais , DNA de Helmintos/química , DNA de Helmintos/isolamento & purificação , Feminino , Helmintos/classificação , Helmintos/genética , Helmintos/isolamento & purificação , Japão , Masculino , Filogenia , Reação em Cadeia da Polimerase , RNA Ribossômico 18S/química , RNA Ribossômico 18S/genética , RNA Ribossômico 28S/genética , RNA Ribossômico 5,8S/genéticaRESUMO
Stable isotopes of carbon and nitrogen characterize trophic relationships in predator-prey relationships, with clear differences between consumer and diet (discrimination factor Δ13C and Δ15N). However, parasite-host isotopic relationships remain unclear, with Δ13C and Δ15N remaining incompletely characterized, especially for helminths. In this study, we used stable isotopes to determine discrimination factors for 13 parasite-host pairings of helminths in coral reef fish. Differences in Δ15N values grouped according to parasite groups and habitat within the host with positive Δ15N values observed for trematodes and nematodes from the digestive tract and variable Δ15N values observed for cestodes and nematodes from the general cavity. Furthermore, Δ13C values showed more complex patterns with no effect of parasite group or habitat within host. A negative relationship was observed between Δ15N and host δ15N values among different host-parasite pairings as well as within 7 out of the 13 pairings, indicating that host metabolic processing affects host-parasite discrimination values. In contrast, no relationships were observed for Δ13C values. Our results indicate that parasite group, habitat within host, and host stable isotope value drive Δ15N of helminths in coral reef fish while their effect on Δ13C is more idiosyncratic. These results call for use of taxon- or species-specific and scaled framework for bulk stable isotopes in the trophic ecology of parasites.
Assuntos
Recifes de Corais , Ecossistema , Peixes/parasitologia , Helmintos/patogenicidade , Animais , Interações Hospedeiro-Parasita , Isótopos/análiseRESUMO
Helminths can interact with their hosts in many different ways, including through the secretion of soluble molecules (such as lipids, glycans and proteins) and extracellular vesicles (EVs). The field of helminth secreted EVs has significantly advanced in recent years, mainly due to the molecular characterisation of EV proteomes and research highlighting the potential of EVs and their constituent molecules in the diagnosis and control of parasitic infections. Despite these advancements, the lack of appropriate isolation and purification methods is impeding the discovery of suitable biomarkers for the differentiation of helminth EV populations. In the present review we offer our viewpoint on the different proteomic techniques and approaches that have been developed, as well as solutions to common pitfalls and challenges that could be applied to advance the study of helminth EVs.
Assuntos
Vesículas Extracelulares/metabolismo , Helmintos/metabolismo , Proteoma/metabolismo , Animais , Biomarcadores/metabolismo , Helmintos/patogenicidade , Doenças Parasitárias/parasitologia , Proteômica/métodosRESUMO
BACKGROUND: Soil-transmitted helminths (STH), i.e., Ascaris lumbricoides, Trichuris trichiura and hookworms are among the most prevalent Neglected Tropical Diseases (NTDs) in Ethiopia. Although pre-school aged children pay a high morbidity toll associated with STH infections, evidence on prevalence, intensity and intervention status is lacking in Ethiopia. This study, therefore, aimed to address these gaps to inform decision made on STH. METHODS: We did a community-based cross-sectional study in five districts of Gamo Gofa zone, Southern Ethiopia; in January 2019. Data were collected using pre-tested questionnaire, and the Kato-Katz technique was used to diagnose parasites eggs in stool. Then, collected data were edited and entered into EpiData 4.4.2, and exported to SPSS software (IBM, version 25) for analysis. RESULTS: A total of 2462 PSAC participated in this study. Overall, the prevalence of STH was 23.5% (578/2462) (95% confidence interval (CI) = 21.8%-25.2%). As caris lumbricoides was the most prevalent (18.6%), followed by Trichuris trichiura (9.2%), and hookworms (3.1%). Of the total, 7.4% PSAC were infected with two STH species. Most of the positive cases with STH showed low infection intensities, while 15.1% ascariasis cases showed moderate infection intensities. The study found that 68.7% of PSAC were treated with albendazole. Also, household's level data showed that 39.4% used water from hand-dug well; 52.5% need to travel ≥30 minutes to collect water; 77.5% did not treat water, and 48.9% had no hand washing facility. In addition, almost 93% care givers achieved less than the mean knowledge and practice score (≤5) on STH prevention. CONCLUSIONS: This study showed that significant proportions of pre-school aged children are suffering from STH infections despite preventive chemotherapy exist at the study area. Also, gaps in the interventions against STH were highlighted. Thus, a call for action is demanding to eliminate STH among PSAC in Ethiopia by 2030.
Assuntos
Ascaríase/transmissão , Helmintíase/transmissão , Solo/parasitologia , Tricuríase/transmissão , Adolescente , Idoso , Ancylostomatoidea/patogenicidade , Animais , Ascaríase/epidemiologia , Ascaríase/parasitologia , Ascaríase/prevenção & controle , Ascaris lumbricoides/patogenicidade , Criança , Pré-Escolar , Estudos Transversais , Etiópia/epidemiologia , Fezes/parasitologia , Feminino , Desinfecção das Mãos , Helmintíase/parasitologia , Helmintíase/patologia , Helmintíase/prevenção & controle , Helmintos/patogenicidade , Humanos , Masculino , Prevalência , Tricuríase/epidemiologia , Tricuríase/parasitologia , Tricuríase/prevenção & controle , Trichuris/patogenicidadeRESUMO
BACKGROUND: Soil-transmitted helminths (STH) remain one of the most common causes of morbidity among children in Ethiopia. Assessment of the magnitude of STH and its association with water, sanitation, and hygiene (WASH) and identify barriers for school-level prevention assist public health planners to prioritize promotion strategies and is a basic step for intervention. However, there is a lack of evidence on the prevalence of STH and its association with WASH and barriers for school-level prevention among schoolchildren. OBJECTIVE: To assess the prevalence of STH and its association with WASH and identify barriers for school level prevention in technology village of Hawassa University; 2019. METHODS: An institution-based analytical cross-sectional study was conducted on a sample of 1080 schoolchildren from September 5 to October 15, 2019. A two-stage cluster and purposive sampling technique were used to draw the study participants. A pretested, structured questionnaire, observation checklist, and in-depth interview were used to collect the data. Two grams of stool samples were collected from each study participant and examined using direct wet mount and Kato-Katz technique. Data were entered into Epi Info version 7 and analyzed using SPSS version 25. Both bi-variable and multivariable logistic regression analyses were done. Qualitative data were analyzed using thematic content analysis method by Atlas-Ti software and presented in narratives. RESULTS: The overall prevalence of STHs was 23.1% (95% CI = 21.4, 27.6). The identified predictors of STHs were large family size (AOR = 2.03; 95% CI = 1.53-3.99), absence of separate toilet room for male and female (AOR = 3.33; 95% CI = 1.91-5.79), toilet not easy to clean (AOR = 2.17; 95% CI = 1.44-3.33), inadequate knowledge about STHs (AOR = 2.08; 95% CI = 1.07-3.44) and children who had travelled greater than 100 meters to access toilet (AOR = 3.45; 95% CI = 2.24-8.92). These results were supported by the individual, institutional, socio-economic and cultural qualitative results. CONCLUSION: The STHs was moderate public health concerns. Reinforcing the existing fragile water, sanitation and hygiene programs and regular deworming of schoolchildren may support to reduce the burden of STHs. Also, increasing modern family planning methods utilization to decrease family size is recommended.
Assuntos
Helmintíase/epidemiologia , Helmintíase/transmissão , Helmintos/patogenicidade , Adolescente , Animais , Criança , Estudos Transversais , Etiópia/epidemiologia , Feminino , Humanos , Higiene , Masculino , Prevalência , Saúde Pública , Saneamento , Serviços de Saúde Escolar , Instituições Acadêmicas/estatística & dados numéricos , Solo/parasitologia , Água/parasitologiaRESUMO
Parasitic helminths have coevolved with humans over millennia, intricately refining and developing an array of mechanisms to suppress or skew the host's immune system, thereby promoting their long-term survival. Some helminths, such as hookworms, cause little to no overt pathology when present in modest numbers and may even confer benefits to their human host. To exploit this evolutionary phenomenon, clinical trials of human helminth infection have been established and assessed for safety and efficacy for a range of immune dysfunction diseases and have yielded mixed outcomes. Studies of live helminth therapy in mice and larger animals have convincingly shown that helminths and their excretory/secretory products possess anti-inflammatory drug-like properties and represent an untapped pharmacopeia. These anti-inflammatory moieties include extracellular vesicles, proteins, glycans, post-translational modifications, and various metabolites. Although the concept of helminth-inspired therapies holds promise, it also presents a challenge to the drug development community, which is generally unfamiliar with foreign biologics that do not behave like antibodies. Identification and characterization of helminth molecules and vesicles and the molecular pathways they target in the host present a unique opportunity to develop tailored drugs inspired by nature that are efficacious, safe, and have minimal immunogenicity. Even so, much work remains to mine and assess this out-of-the-box therapeutic modality. Industry-based organizations need to consider long-haul investments aimed at unraveling and exploiting unique and differentiated mechanisms of action as opposed to toe-dipping entries with an eye on rapid and profitable turnarounds.
Assuntos
Proteínas de Helminto/imunologia , Helmintíase/imunologia , Helmintos/imunologia , Imunomodulação , Animais , Helmintíase/patologia , Helmintíase/terapia , Helmintos/patogenicidade , HumanosRESUMO
There is limited knowledge about parasites of the endangered Ryukyu long-furred rat, Diplothrix legata (Murinae, Rodentia) endemic to Okinawa, Tokunoshima, and Amami-Oshima Islands in Japan. In the present study, postmortem histopathological examination of an individual found on Amami-Oshima Island revealed a mixed helminth infection of Calodium hepaticum, Hydatigera taeniaeformis, and Angiostrongylus cantonensis. These helminths are considered non-native to Amami-Oshima Island and are maintained by invasive mammals, such as non-native rats and outdoor cats. This observation presents a new host record for C. hepaticum and H. taeniaeformis and the first record of A. cantonensis in Ryukyu long-furred rat on Amami-Oshima Island.
Assuntos
Helmintíase Animal/diagnóstico , Helmintíase Animal/transmissão , Helmintos/patogenicidade , Murinae/parasitologia , Animais , Gatos/parasitologia , Coinfecção/parasitologia , Espécies em Perigo de Extinção , Feminino , Helmintos/genética , Ilhas , Japão , Ratos/parasitologia , Análise de Sequência de DNARESUMO
Interactions between co-infecting parasite species can impact transmission. Whether co-infection is beneficial or detrimental to a target parasite, and whether the mechanism involves changes in host susceptibility or parasite clearance, can be difficult to assess. We demonstrate the potential for host age-parasite intensity curves to allow assessment of these factors. A model is developed to generate predictions and test these predictions using helminth parasites of white-tailed deer (Odocoileus virginianus). We identify three beneficial interactions involving five helminth species, including susceptibility and clearance-based mechanisms. Our results suggest that analysis of age-intensity data represents a new tool for assessing the nature and strength of co-infecting parasite interactions.
Assuntos
Coinfecção/parasitologia , Cervos/parasitologia , Helmintos/patogenicidade , Animais , Suscetibilidade a Doenças/parasitologia , Transmissão de Doença Infecciosa , Interações Hospedeiro-Parasita , Modelos TeóricosRESUMO
Parasitic helminths and pest insects are organisms with great ecological importance, having direct or indirect detrimental effects on people's lives worldwide. Several reports in literature indicate that the glycan repertoire of parasites plays important roles in host-parasite interactions and modulation and evasion of the host immune system, while insect glycans are essential for their survival, growth and development. Although glycosylation is the result of a highly conserved machinery, differences between species and between different stages of one organism's life cycle occur. This review provides insight into recent glycomics studies both for helminths and insects, focussing on sex differences and the role of carbohydrate structures in reproduction. Information on the differential N-glycosylation process between males and females can generate a better understanding of the biology and physiology of these economic important organisms, and can contribute to the discovery of novel anti-fecundity vaccine candidates and drug targets, as well as in the elaboration of innovative pest management strategies.
Assuntos
Helmintos/metabolismo , Insetos/metabolismo , Parasitos/metabolismo , Polissacarídeos/metabolismo , Reprodução , Animais , Feminino , Glicosilação , Helmintos/crescimento & desenvolvimento , Helmintos/patogenicidade , Helmintos/fisiologia , Insetos/crescimento & desenvolvimento , Insetos/patogenicidade , Insetos/fisiologia , Masculino , Parasitos/crescimento & desenvolvimento , Parasitos/patogenicidade , Parasitos/fisiologia , Diferenciação SexualRESUMO
Much of our knowledge of the drivers of immune variation, and how these responses vary over time, comes from humans, domesticated livestock or laboratory organisms. While the genetic basis of variation in immune responses have been investigated in these systems, there is a poor understanding of how genetic variation influences immunity in natural, untreated populations living in complex environments. Here, we examine the genetic architecture of variation in immune traits in the Soay sheep of St Kilda, an unmanaged population of sheep infected with strongyle gastrointestinal nematodes. We assayed IgA, IgE and IgG antibodies against the prevalent nematode Teladorsagia circumcincta in the blood plasma of > 3,000 sheep collected over 26 years. Antibody levels were significantly heritable (h2 = 0.21 to 0.57) and highly stable over an individual's lifespan. IgA levels were strongly associated with a region on chromosome 24 explaining 21.1% and 24.5% of heritable variation in lambs and adults, respectively. This region was adjacent to two candidate loci, Class II Major Histocompatibility Complex Transactivator (CIITA) and C-Type Lectin Domain Containing 16A (CLEC16A). Lamb IgA levels were also associated with the immunoglobulin heavy constant loci (IGH) complex, and adult IgE levels and lamb IgA and IgG levels were associated with the major histocompatibility complex (MHC). This study provides evidence of high heritability of a complex immunological trait under natural conditions and provides the first evidence from a genome-wide study that large effect genes located outside the MHC region exist for immune traits in the wild.
Assuntos
Animais Selvagens/imunologia , Imunidade Inata , Ovinos/imunologia , Infecções por Strongylida/imunologia , Animais , Animais Selvagens/sangue , Anticorpos/sangue , Anticorpos/imunologia , Helmintos/imunologia , Helmintos/patogenicidade , Imunoglobulina A/sangue , Imunoglobulina A/imunologia , Imunoglobulina E/sangue , Imunoglobulina E/imunologia , Imunoglobulina G/sangue , Imunoglobulina G/imunologia , Ovinos/sangue , Infecções por Strongylida/sangueRESUMO
Helminth parasites infect more than a quarter of the human population and inflict significant changes to the immunological status of their hosts. Here, we analyze the impact of helminth infections on the efficacy of vaccinations using Litomosoides sigmodontis-infected mice. Concurrent helminth infection reduces the quantity and quality of antibody responses to vaccination against seasonal influenza. Vaccination-induced protection against challenge infections with the human pathogenic 2009 pandemic H1N1 influenza A virus is drastically impaired in helminth-infected mice. Impaired responses are also observed if vaccinations are performed after clearance of a previous helminth infection, suggesting that individuals in helminth-endemic areas may not always benefit from vaccinations, even in the absence of an acute and diagnosable helminth infection. Mechanistically, the suppression is associated with a systemic and sustained expansion of interleukin (IL)-10-producing CD4+CD49+LAG-3+ type 1 regulatory T cells and partially abrogated by in vivo blockade of the IL-10 receptor.
Assuntos
Fatores de Transcrição Forkhead/metabolismo , Helmintos/imunologia , Helmintos/patogenicidade , Vírus da Influenza A Subtipo H1N1/patogenicidade , Influenza Humana/prevenção & controle , Linfócitos T/metabolismo , Vacinação/métodos , Animais , Formação de Anticorpos/genética , Formação de Anticorpos/fisiologia , Fatores de Transcrição Forkhead/genética , Humanos , Imunomodulação/imunologia , Vírus da Influenza A Subtipo H1N1/imunologia , Vacinas contra Influenza/uso terapêutico , Influenza Humana/imunologia , Interleucina-10/metabolismo , Camundongos , Estações do AnoRESUMO
Granuloma formation is a key host immune response generated to confine invading pathogens and limit extensive host damage. It consists of an accumulation of host immune cells around a pathogen. This host response has been extensively studied in the context of inflammatory diseases. However, there is much less known about Th2-type granulomas generated in response to parasitic worms. Based on in vitro data, innate immune cells within the granuloma are thought to immobilize and kill parasites but also act to repair damaged tissue. Understanding this dual function is key. The two billion people and many livestock/wild animals infected with helminths demonstrate that granulomas are not effective at clearing infection. However, the lack of high mortality highlights their importance in ensuring that parasite migration/tissue damage is restricted and wound healing is effective. In this review, we define two key cellular players (macrophages and eosinophils) and their associated molecular players involved in Th2 granuloma function. To date, the underlying mechanisms remain poorly understood, which is in part due to a lack of conclusive studies. Most have been performed in vitro rather than in vivo, using cells that have not been obtained from granulomas. Experiments using genetically modified mouse strains and/or antibody/chemical-mediated cell depletion have also generated conflicting results depending on the model. We discuss the caveats of previous studies and the new tools available that will help fill the gaps in our knowledge and allow a better understanding of the balance between immune killing and healing.
Assuntos
Eosinófilos/imunologia , Granuloma/imunologia , Helmintíase/imunologia , Helmintos/imunologia , Mucosa Intestinal/imunologia , Macrófagos/imunologia , Células Th2/imunologia , Animais , Comunicação Celular , Citocinas/biossíntese , Citocinas/imunologia , Modelos Animais de Doenças , Eosinófilos/parasitologia , Eosinófilos/patologia , Granuloma/parasitologia , Granuloma/patologia , Helmintíase/parasitologia , Helmintíase/patologia , Helmintos/crescimento & desenvolvimento , Helmintos/patogenicidade , Interações Hospedeiro-Parasita/imunologia , Humanos , Imunidade Inata , Mucosa Intestinal/patologia , Macrófagos/parasitologia , Macrófagos/patologia , Camundongos , Células Th2/parasitologia , Células Th2/patologia , Cicatrização/imunologiaRESUMO
Terpenoids play important roles in plant defense. Although some terpene synthases have been characterized, terpenoids and their biosynthesis in wheat (Triticumaestivum L.) still remain largely unknown. Here, we describe the identification of a terpene synthase gene in wheat. It encodes a sesquiterpene synthase that catalyzes ß-patchoulene formation with E,E-farnesyl diphosphate (FPP) as the substrate, thus named as TaPS. TaPS exhibits inducible expression in wheat in response to various elicitations. Particularly, alamethicin treatment strongly induces TaPS gene expression and ß-patchoulene accumulation in wheat. Overexpression of TaPS in Arabidopsis successfully produces ß-patchoulene, verifying the biochemical function of TaPS in planta. Furthermore, these transgenic Arabidopsis plants exhibit resistance against herbivory by repelling beet armyworm larvae feeding, thereby indicating anti-herbivory activity of ß-patchoulene. The catalytic mechanism of TaPS is also explored by homology modeling and site-directed mutagenesis. Two key amino acids are identified to act in protonation and stability of intermediates and product formation. Taken together, one wheat sesquiterpene synthase is identified as ß-patchoulene synthase. TaPS exhibits inducible gene expression and the sesquiterpene ß-patchoulene is involved in repelling insect infestation.
Assuntos
Alquil e Aril Transferases/genética , Arabidopsis/genética , Plantas Geneticamente Modificadas/genética , Triticum/genética , Alquil e Aril Transferases/química , Alquil e Aril Transferases/farmacologia , Animais , Arabidopsis/química , Regulação da Expressão Gênica de Plantas/genética , Helmintos/efeitos dos fármacos , Helmintos/patogenicidade , Herbivoria/efeitos dos fármacos , Herbivoria/genética , Plantas Geneticamente Modificadas/crescimento & desenvolvimento , Sesquiterpenos de Guaiano/metabolismoRESUMO
BACKGROUND: Asthma is a chronic disease of the airways and its most common phenotype is characterized by a T2 type response with IgE production and inflammatory mediators in response to common allergens. Cysteinyl leukotrienes (CysLTs), LTC4, LTD4 and LTE4, are mediators known to possess important proinflammatory action. CysLTs can bind to the Cysteinyl leukotriene receptor type 2 (CysLTR2) and activate an inflammatory. Polymorphisms in CysLTR2 have been associated with asthma and atopy, although the mechanism is not clear. OBJECTIVE: To evaluate the association between genetic polymorphisms in CYSLTR2 with asthma phenotypes, atopy markers and helminth infection. METHODS: Genotyping was performed using a panel Illumina and carried out in 1245 participants of SCAALA program (Social Change, Asthma, Allergy in Latin American). Logistic regressions for asthma, helminth infections (Trichuris trichiura and Ascaris lumbricoides) and allergy markers (skin tests and IgE production) were performed using PLINK 1.9 software adjusted for sex, age, helminth infection and ancestry markers. RESULTS: The G allele of rs1323556 was negatively associated with asthma in the additive model (OR 0.74, 95% CI 0.59-0.93) and in the dominant model (OR 0.71, 95% CI 0.53-0.74). The G allele of rs1575464 was also negatively associated with asthma in two genetic models, additive (OR 0.77, 95% CI 0.62-0.96) and dominant (OR 0.73, 95% CI 0.55-0.97). The G allele of rs61735175 was positively associated with asthma severity in the additive model (OR 1.72, 95% CI 1.07-2.77) and in the dominant model (OR 1.77, 95% CI 1.09-2.85). Five SNVs were associated with atopy markers and four SNVs were associated with helminth infections. CONCLUSION: Polymorphisms in the CYSLTR2 gene are associated with asthma, atopy markers and helminth infection in Brazilian individuals, which may lead to protection or risk for such conditions, however, more studies are needed to evaluate the functional of this variants here in described.
Assuntos
Asma/genética , Predisposição Genética para Doença , Hipersensibilidade Imediata/genética , Receptores de Leucotrienos/genética , Alérgenos/genética , Alérgenos/imunologia , Animais , Asma/epidemiologia , Asma/parasitologia , Criança , Pré-Escolar , Feminino , Estudos de Associação Genética , Genótipo , Helmintíase/epidemiologia , Helmintíase/genética , Helmintíase/parasitologia , Helmintos/genética , Helmintos/patogenicidade , Humanos , Hipersensibilidade , Hipersensibilidade Imediata/parasitologia , Inflamação/epidemiologia , Inflamação/genética , Inflamação/parasitologia , Masculino , Fenótipo , Polimorfismo de Nucleotídeo Único/genéticaRESUMO
Group 2 innate lymphoid cells (ILC2) have emerged as a major component of type 2 inflammation in mice and humans. ILC2 secrete large amounts of interleukins 5 and 13, which are largely responsible for host protective immunity against helminth parasites because these cytokines induce profound changes in host physiology that include: goblet cell metaplasia, mucus accumulation, smooth muscle hypercontractility, eosinophil and mast cell recruitment, and alternative macrophage activation (M2). This review covers the initial recognition of ILC2 as a distinct cell lineage, the key studies that established their biological importance, particularly in helminth infection, and the new directions that are likely to be the focus of emerging work that further explores this unique cell population in the context of health and disease.