RESUMO
BACKGROUND: Ischemic stroke is a major health issue that causes high incidents of morbidity and mortality worldwide. Irisin is an excise-induced protein that has exhibited pleiotropic properties. Accumulating evidence reveals its critical roles in the regulation of various cellular functions, including nervous system functions. This study aims to disclose the effect of irisin on rat cerebral neurons suffering from hypoxia/reoxygenation (H/R) treatment and to explore the potential underlying molecular mechanisms. METHODS: The percentage of rat cerebral neuron cell death was determined by flow cytometry analysis and MTT assay. The expression levels of target genes were measured by western blotting and real-time quantitative reverse transcription PCR assay. RESULTS: Our results demonstrated that irisin treatment substantially reduced H/R-induced apoptosis of rat cerebral neurons. Further investigation revealed that irisin treatment markedly decreased mitogen-activated protein kinase (MAPK) signaling pathway activation and suppressed pro-informatory cytokine expression in cerebral neurons with H/R challenge. Finally, we showed that the neuroprotective effect and anti-inflammatory effect of irisin were comparable with three MAPK signaling inhibitors. CONCLUSION: Irisin exerts profound neuroprotective and anti-inflammatory effects on H/R-stimulated cerebral neurons by inhibiting the MAPK signaling activation. Therefore, irisin may serve as a potential drug for the treatment of patients with ischemic stroke.
Assuntos
Fibronectinas , AVC Isquêmico , Animais , Ratos , Anti-Inflamatórios/imunologia , Anti-Inflamatórios/farmacologia , Apoptose/genética , Apoptose/imunologia , Citocinas/genética , Citocinas/imunologia , Fibronectinas/genética , Fibronectinas/imunologia , Fibronectinas/farmacologia , Hipóxia Encefálica/genética , Hipóxia Encefálica/imunologia , AVC Isquêmico/genética , AVC Isquêmico/imunologia , Neurônios/imunologiaRESUMO
Hypoxia (HYPX) induced-overload Ca2+ entry results in increase of mitochondrial oxidative stress, inflammation and apoptosis in several neurons. Ca2+ permeable TRPM2 channel was gated by ADP-ribose (ADPR) and reactive oxygen species (ROS), although its activity was modulated in HYPX-exposed neurons by resveratrol (RSV). The aim of this study was to evaluate if a therapy of RSV can modulate the effect of HYPX in the TRPM2 expressing SH-SY5Y neuronal and HEK293 (no expression of TRPM2) cell lines. The SH-SY5Y and HEK293 cells were divided into four groups as control, RSV (50 µM and 24 hours), and HYPX and RSV + HYPX. For induction of HYPX in the cells, CoCl2 (200 µM and 24 hours) incubation was used. HYPX-induced intracellular Ca2+ responses to TRPM2 activation were increased in the SH-SY5Y cells but not in the HEK293 cells from coming H2O2 and ADPR. RSV treatment improved intracellular Ca2+ responses, mitochondrial function, suppressed the generation of cytokine (IL-1ß and TNF-α), cytosolic and mitochondrial ROS in the SH-SY5Y cells. Intracellular free Zn2+, apoptosis, cell death, PARP-1, TRPM2 expression, caspase -3 and -9 levels are increased through activating TRPM2 in the SH-SY5Y cells exposed to the HYPX. However, the values were decreased in the cells by RSV and TRPM2 blockers (ACA and 2-APB). In SH-SY5Y neuronal cells exposed to HYPX conditions, the neuroprotective effects of RSV were shown to be exerted via modulation of oxidative stress, inflammation, apoptosis and death through modulation of TRPM2 channel. RSV could be used as an effective agent in the treatment of neurodegeneration exposure to HYPX.
Assuntos
Apoptose/efeitos dos fármacos , Mitocôndrias/efeitos dos fármacos , Neurônios/efeitos dos fármacos , Resveratrol/farmacologia , Canais de Cátion TRPM/metabolismo , Adenosina Difosfato Ribose/metabolismo , Hipóxia Celular/efeitos dos fármacos , Hipóxia Celular/imunologia , Linhagem Celular Tumoral , Cobalto/toxicidade , Células HEK293 , Humanos , Peróxido de Hidrogênio/metabolismo , Hipóxia Encefálica/tratamento farmacológico , Hipóxia Encefálica/imunologia , Hipóxia Encefálica/patologia , Mitocôndrias/metabolismo , Mitocôndrias/patologia , Neurônios/citologia , Neurônios/imunologia , Neurônios/patologia , Estresse Oxidativo/efeitos dos fármacos , Estresse Oxidativo/imunologia , Resveratrol/uso terapêuticoRESUMO
Moderate anemia is associated with increased mortality and morbidity, including acute kidney injury (AKI), in surgical patients. A red blood cell (RBC)-specific antibody model was utilized to determine whether moderate subacute anemia could result in tissue hypoxia as a potential mechanism of injury. Cardiovascular and hypoxic cellular responses were measured in transgenic mice capable of expressing hypoxia-inducible factor-1α (HIF-1α)/luciferase activity in vivo. Antibody-mediated anemia was associated with mild intravascular hemolysis (6 h) and splenic RBC sequestration ( day 4), resulting in a nadir hemoglobin concentration of 89 ± 13 g/l on day 4. At this time point, renal tissue oxygen tension (PtO2) was decreased in anemic mice relative to controls (13.1 ± 4.3 vs. 20.8 ± 3.7 mmHg, P < 0.001). Renal tissue hypoxia was associated with an increase in HIF/luciferase expression in vivo ( P = 0.04) and a 20-fold relative increase in renal erythropoietin mRNA transcription ( P < 0.001) but no increase in renal blood flow ( P = 0.67). By contrast, brain PtO2 was maintained in anemic mice relative to controls (22.7 ± 5.2 vs. 23.4 ± 9.8 mmHg, P = 0.59) in part because of an increase in internal carotid artery blood flow (80%, P < 0.001) and preserved cerebrovascular reactivity. Despite these adaptive changes, an increase in brain HIF-dependent mRNA levels was observed (erythropoietin: P < 0.001; heme oxygenase-1: P = 0.01), providing evidence for subtle cerebral tissue hypoxia in anemic mice. These data demonstrate that moderate subacute anemia causes significant renal tissue hypoxia, whereas adaptive cerebrovascular responses limit the degree of cerebral tissue hypoxia. Further studies are required to assess whether hypoxia is a mechanism for acute kidney injury associated with anemia.
Assuntos
Injúria Renal Aguda/sangue , Anemia/sangue , Anticorpos Monoclonais , Encéfalo/irrigação sanguínea , Eritrócitos/metabolismo , Hipóxia Encefálica/sangue , Rim/irrigação sanguínea , Oxigênio/sangue , Injúria Renal Aguda/imunologia , Injúria Renal Aguda/patologia , Injúria Renal Aguda/fisiopatologia , Anemia/imunologia , Anemia/patologia , Anemia/fisiopatologia , Animais , Encéfalo/metabolismo , Encéfalo/patologia , Circulação Cerebrovascular , Modelos Animais de Doenças , Eritrócitos/imunologia , Eritrócitos/patologia , Eritropoetina/genética , Eritropoetina/metabolismo , Glicoforinas/sangue , Glicoforinas/imunologia , Hemólise , Hipóxia Encefálica/imunologia , Hipóxia Encefálica/patologia , Hipóxia Encefálica/fisiopatologia , Subunidade alfa do Fator 1 Induzível por Hipóxia/genética , Subunidade alfa do Fator 1 Induzível por Hipóxia/metabolismo , Rim/metabolismo , Rim/patologia , Masculino , Camundongos Transgênicos , Circulação Renal , Índice de Gravidade de Doença , Baço/metabolismo , Baço/patologia , Regulação para CimaRESUMO
Hypoxic-ischemic brain damage (HIBD) is a common cause of infant death. The purpose of our research was to explore the immunoregulatory mechanism of placenta-derived mesenchymal stem cells (PD-MSCs) in HIBD treatment. Seven-day-old rat pups were randomly divided into HIBD, PD-MSC, fibroblast, and control groups. Forty-eight hours after HIBD induction, cells at a density of 5 × 104 cells/10 µl were injected into the cerebral tissue in the PD-MSC and fibroblast groups. The TNF-α, interleukin- 17 (IL-17), interferon-γ (IFN-γ), and IL-10 levels were detected through quantitative real-time polymerase chain reaction (RT-PCR) and enzyme-linked immunosorbent assay (ELISA). Regulatory T cell (Tregs) populations were detected through flow cytometry, and forkhead box P3 (Foxp3) was measured through western blot analysis. Behavioral tests and gross and pathological examinations showed that PD-MSC treatment exerted significantly stronger neuroprotective effects than the other treatments. The expression levels of pro-inflammatory cytokines were substantially upregulated after HI injury. Compared with fibroblast treatment, PD-MSC treatment inhibited the production of pro-inflammatory cytokines and increased the production of IL-10 in the ischemic hemispheres and peripheral blood serum (all P < 0.01). Flow cytometry results showed a notable increase in the number of Tregs within the spleen of the HIBD group. Moreover, the number of Tregs and the Foxp3 expression levels were higher in the PD-MSC treatment group than in the HIBD and fibroblast groups (all P < 0.01). Our research suggests that the mechanism of PD-MSC treatment for HIBD partially involves inflammatory response suppression.
Assuntos
Hipóxia Encefálica/terapia , Hipóxia/terapia , Inflamação/terapia , Isquemia/terapia , Transplante de Células-Tronco Mesenquimais , Células-Tronco Mesenquimais/fisiologia , Placenta/citologia , Linfócitos T Reguladores/imunologia , Animais , Animais Recém-Nascidos , Escala de Avaliação Comportamental , Citocinas/metabolismo , Feminino , Fatores de Transcrição Forkhead/metabolismo , Regulação da Expressão Gênica , Humanos , Hipóxia/imunologia , Hipóxia Encefálica/imunologia , Terapia de Imunossupressão , Lactente , Inflamação/imunologia , Isquemia/imunologia , Neuroproteção , Gravidez , RatosRESUMO
Inflammatory activation of microglia is a hallmark of several disorders of the central nervous system. In addition to protecting the brain against inflammatory insults, microglia are neuroprotective and play a significant role in maintaining neuronal connectivity, but the prolongation of an inflammatory status may limit the beneficial functions of these immune cells. The finding that estrogen receptors are present in monocyte-derived cells and that estrogens prevent and control the inflammatory response raise the question of the role that this sex steroid plays in the manifestation and progression of pathologies that have a clear sex difference in prevalence, such as multiple sclerosis, Parkinson's disease, and Alzheimer's disease. The present review aims to provide a critical review of the current literature on the actions of estrogen in microglia and on the involvement of estrogen receptors in the manifestation of selected neurological disorders. This current understanding highlights a research area that should be expanded to identify appropriate replacement therapies to slow the progression of such diseases.
Assuntos
Doenças Desmielinizantes , Estrogênios/metabolismo , Hipóxia Encefálica , Inflamação , Microglia , Doenças Neurodegenerativas , Receptores de Estrogênio/metabolismo , Acidente Vascular Cerebral , Animais , Doenças Desmielinizantes/imunologia , Doenças Desmielinizantes/metabolismo , Humanos , Hipóxia Encefálica/imunologia , Hipóxia Encefálica/metabolismo , Inflamação/imunologia , Inflamação/metabolismo , Microglia/imunologia , Microglia/metabolismo , Doenças Neurodegenerativas/imunologia , Doenças Neurodegenerativas/metabolismo , Acidente Vascular Cerebral/imunologia , Acidente Vascular Cerebral/metabolismoRESUMO
Transient hypoxia in pregnancy stimulates a physiological reflex response that redistributes blood flow and defends oxygen delivery to the fetal brain. We designed the present experiment to test the hypotheses that transient hypoxia produces damage of the cerebral cortex and that ketamine, an antagonist ofNMDAreceptors and a known anti-inflammatory agent, reduces the damage. Late gestation, chronically catheterized fetal sheep were subjected to a 30-min period of ventilatory hypoxia that decreased fetal PaO2from 17 ± 1 to 10 ± 1 mmHg, or normoxia (PaO217 ± 1 mmHg), with or without pretreatment (10 min before hypoxia/normoxia) with ketamine (3 mg/kg, i.v.). One day (24 h) after hypoxia/normoxia, fetal cerebral cortex was removed andmRNAextracted for transcriptomics and systems biology analysis (n = 3-5 per group). Hypoxia stimulated a transcriptomic response consistent with a reduction in cellular metabolism and an increase in inflammation. Ketamine pretreatment reduced both of these responses. The inflammation response modeled with transcriptomic systems biology was validated by immunohistochemistry and showed increased abundance of microglia/macrophages after hypoxia in the cerebral cortical tissue that ketamine significantly reduced. We conclude that transient hypoxia produces inflammation of the fetal cerebral cortex and that ketamine, in a standard clinical dose, reduces the inflammation response.
Assuntos
Anti-Inflamatórios/farmacologia , Córtex Cerebral/efeitos dos fármacos , Hipóxia Fetal/tratamento farmacológico , Hipóxia Encefálica/tratamento farmacológico , Mediadores da Inflamação/metabolismo , Ketamina/farmacologia , Fármacos Neuroprotetores/farmacologia , Animais , Córtex Cerebral/imunologia , Córtex Cerebral/metabolismo , Córtex Cerebral/patologia , Modelos Animais de Doenças , Feminino , Hipóxia Fetal/genética , Hipóxia Fetal/imunologia , Hipóxia Fetal/metabolismo , Hipóxia Fetal/patologia , Perfilação da Expressão Gênica/métodos , Regulação da Expressão Gênica , Idade Gestacional , Hipóxia Encefálica/genética , Hipóxia Encefálica/imunologia , Hipóxia Encefálica/metabolismo , Hipóxia Encefálica/patologia , Mediadores da Inflamação/imunologia , Análise de Sequência com Séries de Oligonucleotídeos , Gravidez , RNA Mensageiro/metabolismo , Ovinos , Biologia de Sistemas , Fatores de TempoRESUMO
Neonatal brain hypoxic ischemia (HI) often results in long-term motor and cognitive impairments. Post-ischemic inflammation greatly effects outcome and adenosine receptor signaling modulates both HI and immune cell function. Here, we investigated the influence of adenosine A1 receptor deficiency (A1R(-/-)) on key immune cell populations in a neonatal brain HI model. Ten-day-old mice were subjected to HI. Functional outcome was assessed by open locomotion and beam walking test and infarction size evaluated. Flow cytometry was performed on brain-infiltrating cells, and semi-automated analysis of flow cytometric data was applied. A1R(-/-) mice displayed larger infarctions (+33%, p < 0.05) and performed worse in beam walking tests (44% more mistakes, p < 0.05) than wild-type (WT) mice. Myeloid cell activation after injury was enhanced in A1R(-/-) versus WT brains. Activated B lymphocytes expressing IL-10 infiltrated the brain after HI in WT, but were less activated and did not increase in relative frequency in A1R(-/-). Also, A1R(-/-) B lymphocytes expressed less IL-10 than their WT counterparts, the A1R antagonist DPCPX decreased IL-10 expression whereas the A1R agonist CPA increased it. CD4(+) T lymphocytes including FoxP3(+) T regulatory cells, were unaffected by genotype, whereas CD8(+) T lymphocyte responses were smaller in A1R(-/-) mice. Using PCA to characterize the immune profile, we could discriminate the A1R(-/-) and WT genotypes as well as sham operated from HI-subjected animals. We conclude that A1R signaling modulates IL-10 expression by immune cells, influences the activation of these cells in vivo, and affects outcome after HI.
Assuntos
Isquemia Encefálica/imunologia , Encéfalo/imunologia , Hipóxia Encefálica/imunologia , Receptor A1 de Adenosina/imunologia , Agonistas do Receptor A1 de Adenosina/farmacologia , Antagonistas do Receptor A1 de Adenosina/farmacologia , Animais , Animais Recém-Nascidos , Linfócitos T CD4-Positivos/efeitos dos fármacos , Linfócitos T CD4-Positivos/metabolismo , Infarto Cerebral/patologia , Feminino , Hipóxia Encefálica/congênito , Interleucina-10/biossíntese , Interleucina-10/genética , Masculino , Camundongos , Camundongos Endogâmicos C57BL , Camundongos Knockout , Atividade Motora , Células Mieloides/efeitos dos fármacos , Células Mieloides/imunologia , Células Mieloides/metabolismo , Equilíbrio Postural , Gravidez , Receptor A1 de Adenosina/efeitos dos fármacosRESUMO
AIM: Circulating immune cell activation is associated with worse outcome in adult and animal models of brain injury. Our aim was to profile the systemic inflammatory response over the first week of life in infants at risk of neonatal encephalopathy (NE) and correlate early neutrophil and monocyte endotoxin and activation responses with outcome. METHODS: Prospective observational study in a tertiary referral university hospital including 22 infants requiring resuscitation at birth who had serial (five time points) neutrophil and monocyte CD11b (marker of cell adhesion), intracellular reactive oxygen intermediates (ROI; cell activation) and Toll-like receptor (TLR; endotoxin recognition) before and after endotoxin stimulation ex vivo compared to neonatal controls. RESULTS: All neonates requiring resuscitation at delivery (n = 122 samples) had higher neutrophil and monocyte CD11b and TLR-4 expression compared with adults and neonatal controls. Neonates with abnormal neuroimaging and/or severe NE had increased CD11b, ROI and TLR-4. Increased polymorphonuclear leukocytes TLR-4 expression was associated with increased mortality in infants with NE. CONCLUSION: Innate immune dysregulation in the first week of life is associated with severity of outcome in neonatal brain injury in this cohort and may be amenable to immunomodulation.
Assuntos
Antígeno CD11b/metabolismo , Hipóxia Encefálica/imunologia , Fagócitos/metabolismo , Espécies Reativas de Oxigênio/metabolismo , Receptor 4 Toll-Like/metabolismo , Adulto , Feminino , Humanos , Recém-Nascido , Masculino , Ativação de Neutrófilo , Estudos Prospectivos , Estudos RetrospectivosAssuntos
Asfixia Neonatal/terapia , Líquido da Lavagem Broncoalveolar/química , Hipotermia Induzida , Hipóxia Encefálica/terapia , Asfixia Neonatal/imunologia , Estudos de Casos e Controles , Humanos , Hipóxia Encefálica/imunologia , Recém-Nascido , Inflamação , Interleucina-10/imunologia , Interleucina-1beta/imunologia , Interleucina-6/imunologia , Interleucina-8/imunologia , Surfactantes Pulmonares , Resultado do Tratamento , Fator de Necrose Tumoral alfa/imunologiaRESUMO
Guanosine (GUO) is an endogenous modulator of glutamatergic excitotoxicity and has been shown to promote neuroprotection in in vivo and in vitro models of neurotoxicity. This study was designed to understand the neuroprotective mechanism of GUO against oxidative damage promoted by oxygen/glucose deprivation and reoxygenation (OGD). GUO (100 µM) reduced reactive oxygen species production and prevented mitochondrial membrane depolarization induced by OGD. GUO also exhibited anti-inflammatory actions as inhibition of nuclear factor kappa B activation and reduction of inducible nitric oxide synthase induction induced by OGD. These GUO neuroprotective effects were mediated by adenosine A1 receptor, phosphatidylinositol-3 kinase and MAPK/ERK. Furthermore, GUO recovered the impairment of glutamate uptake caused by OGD, an effect that occurred via a Pertussis toxin-sensitive G-protein-coupled signaling, blockade of adenosine A2A receptors (A2A R), but not via A1 receptor. The modulation of glutamate uptake by GUO also involved MAPK/ERK activation. In conclusion, GUO, by modulating adenosine receptor function and activating MAPK/ERK, affords neuroprotection of hippocampal slices subjected to OGD by a mechanism that implicates the following: (i) prevention of mitochondrial membrane depolarization, (ii) reduction of oxidative stress, (iii) regulation of inflammation by inhibition of nuclear factor kappa B and inducible nitric oxide synthase, and (iv) promoting glutamate uptake.
Assuntos
Encefalite , Guanosina/metabolismo , Hipocampo/imunologia , Hipocampo/metabolismo , Hipóxia Encefálica , Animais , Sobrevivência Celular/efeitos dos fármacos , Sobrevivência Celular/fisiologia , Encefalite/tratamento farmacológico , Encefalite/imunologia , Encefalite/metabolismo , Subunidades alfa Gi-Go de Proteínas de Ligação ao GTP/metabolismo , Glucose/farmacologia , Ácido Glutâmico/farmacocinética , Guanosina/farmacologia , Hipocampo/citologia , Hipóxia Encefálica/tratamento farmacológico , Hipóxia Encefálica/imunologia , Hipóxia Encefálica/metabolismo , Sistema de Sinalização das MAP Quinases/efeitos dos fármacos , Sistema de Sinalização das MAP Quinases/imunologia , Masculino , Potencial da Membrana Mitocondrial/fisiologia , NF-kappa B/metabolismo , Proteínas do Tecido Nervoso/metabolismo , Fármacos Neuroprotetores/farmacologia , Óxido Nítrico Sintase Tipo II/metabolismo , Técnicas de Cultura de Órgãos , Estresse Oxidativo/efeitos dos fármacos , Estresse Oxidativo/fisiologia , Oxigênio/farmacologia , Fosfatidilinositol 3-Quinases/metabolismo , Ratos , Ratos Wistar , Espécies Reativas de Oxigênio/metabolismo , Receptor A1 de Adenosina/metabolismo , Sinaptotagminas , TrítioRESUMO
In this article there are materials, which were received in the process of treatment of 69 patients with azaleptin (leponeks) acute severe poisonings. The immunologic research has shown that the first phase of acute poisonings in patients with toxicohypoxic encephalopathy is accompanied by severe immune alterations. The alterations of proinflammation and antiinflammation cytokines have been investigated and its increase has not been revealed. It was shown, that the employment of citoflavin in intensive therapy of acute poisonings leads to decrease of the level of immunosupression.
Assuntos
Mononucleotídeo de Flavina/administração & dosagem , Hipóxia Encefálica/patologia , Sistema Imunitário/patologia , Inosina Difosfato/administração & dosagem , Síndromes Neurotóxicas/patologia , Niacinamida/administração & dosagem , Intoxicação/terapia , Succinatos/administração & dosagem , Contagem de Células Sanguíneas , Clozapina/uso terapêutico , Estado Terminal/terapia , Combinação de Medicamentos , Feminino , Humanos , Hipóxia Encefálica/sangue , Hipóxia Encefálica/induzido quimicamente , Hipóxia Encefálica/imunologia , Masculino , Síndromes Neurotóxicas/sangue , Síndromes Neurotóxicas/imunologiaRESUMO
The aim of this study was to investigate the comparative effects of transforming growth factor ß1 (TGF-ß1) and interleukin-10 (IL-10) on the repeated brief hypoxia-induced alterations in the activity of hippocampal slice CA1 pyramidal neurons. The method of field potentials measurement in CA1 region of hippocampal slices was used. The principal results of our work are summarized as follow. 1. TGF-ß1 reduces the depressive effect of brief hypoxia on the population spike amplitude more effectively than IL-10. 2. During TGF-ß1 exposure (in contrast to IL-10), three 3-min hypoxic episodes do not induce the rapid hypoxic preconditioning. 3. TGF-ß1 and IL-10 equally abolish posthypoxic hyperexcitability induced by repeated brief episodes of hypoxia in CA1 pyramidal neurons. These findings indicated that TGF-ß1 and IL-10 are able to evoke anti-hypoxic effect and abolish the development of posthypoxic hyperexcitability induced by repeated brief hypoxic episodes in hippocampal CA1 pyramidal neurons. Our results also demonstrated that TGF-ß1 reduced the effectiveness of hypoxia to depress neuronal activity more effectively than IL-10. We suggest that the present findings allow to explain the certain neuroprotective mechanisms of IL-10 and TGF-beta1 in the early phase of hypoxia and indicate that a therapeutic anti-inflammatory approach using these substances can provide neuroprotection in the brain hypoxic conditions.
Assuntos
Anti-Inflamatórios/farmacologia , Região CA1 Hipocampal , Hipóxia Encefálica , Interleucina-10/farmacologia , Fator de Crescimento Transformador beta1/farmacologia , Animais , Região CA1 Hipocampal/efeitos dos fármacos , Região CA1 Hipocampal/imunologia , Região CA1 Hipocampal/fisiopatologia , Hipóxia Encefálica/tratamento farmacológico , Hipóxia Encefálica/imunologia , Hipóxia Encefálica/fisiopatologia , Precondicionamento Isquêmico/métodos , Masculino , Potenciais da Membrana/efeitos dos fármacos , Técnicas de Cultura de Órgãos , Células Piramidais/efeitos dos fármacos , Células Piramidais/fisiologia , Ratos , Ratos WistarRESUMO
R(-) enantiomers of the 2-arylpropionic acid derivatives ibuprofen and flurbiprofen weakly inhibit cyclooxygenase (COX) activity. However, a possible cytoprotective effect has been proposed. The aim of the study is to investigate the possible mechanism of this effect. An in vitro hypoxia-reoxygenation model in rat brain slices was used (n=6 rats per group). After reoxygenation, we measured LDH efflux (neuronal death), brain prostaglandin E(2) (PGE(2)) concentration, interleukins (IL)-1ß and 10, oxidative and nitrosative stress (lipid peroxides, glutathione, 3-nitrotyrosine, and nitrites/nitrates). Anti-COX activity was measured in human whole blood. Racemic, R(-), and S(+) enantiomers of ibuprofen and flurbiprofen were tested. All compounds had a cytoprotective effect with IC(50) values in the range of 10(-5) M. R(-) enantiomers did not significantly inhibit brain PGE(2). The concentration of IL-1ß was reduced by 53.1% by the racemic form, 30.6% by the S(+) and 43.2% by the R(-) enantiomer of ibuprofen. The IL-10 concentration increased significantly only with S(+)-flurbiprofen (33.1%) and R(-)-flurbiprofen (26.1%). Lipid peroxidation was significantly reduced by all three forms of flurbiprofen. Nitrite + nitrate concentrations were reduced by racemic, S(+), and R(-)-flurbiprofen. Peroxynitrite formation (3-nitrotyrosine) was significantly reduced by racemic and S(+)-ibuprofen. COX inhibition is not the main mechanism of cytoprotection for these compounds. Their influence on inflammatory mediators and oxidative and nitrosative stress could account for the potential cytoprotective effect of R(-) enantiomers.
Assuntos
Encéfalo/efeitos dos fármacos , Inibidores de Ciclo-Oxigenase/farmacologia , Citoproteção/efeitos dos fármacos , Flurbiprofeno/farmacologia , Ibuprofeno/farmacologia , Prostaglandina-Endoperóxido Sintases/metabolismo , Animais , Encéfalo/enzimologia , Encéfalo/imunologia , Encéfalo/patologia , Inibidores de Ciclo-Oxigenase/química , Citocinas/metabolismo , Dinoprostona/metabolismo , Flurbiprofeno/química , Hipóxia Encefálica/enzimologia , Hipóxia Encefálica/imunologia , Hipóxia Encefálica/patologia , Hipóxia Encefálica/prevenção & controle , Ibuprofeno/química , Técnicas In Vitro , Peroxidação de Lipídeos/efeitos dos fármacos , Masculino , Ratos , Ratos Wistar , Estereoisomerismo , Tirosina/análogos & derivados , Tirosina/análiseRESUMO
The developing brain is susceptible to hypoxic damage because of its high oxygen and energy requirements. Hypoxia-induced inflammatory response has been recognized as one of the main culprits in the development of hypoxic brain injury. In this regard, a hallmark feature is microglial activation which results in overproduction of inflammatory cytokines, free radicals and nitric oxide. Concomitantly, activated microglia exhibit enhanced expression of ion channels such as Kv1.2, Kv1.1 and Nav which further promote the release of inflammatory cytokines, chemokines and reactive oxygen species. Through the above-mentioned inflammatory mediators, activated microglia induce neuronal loss, axonal damage and oligodendroglial death along with myelination disturbances. Our recent studies have extended that tumor necrosis factor-alpha, interleukin-1beta, monocyte chemoattractant protein-1 and macrophage colony stimulating factor produced by activated microglia are linked to the pathogenesis of periventricular white matter damage in the hypoxic brain. It is envisaged that a better understanding of the interactions between microglia and neurons, axons and oligodendrocytes is key to the development of effective preventive and therapeutic strategies for mitigation of hypoxic brain injury.
Assuntos
Encéfalo/crescimento & desenvolvimento , Hipóxia Encefálica/etiologia , Hipóxia Encefálica/imunologia , Hipóxia Encefálica/patologia , Microglia/citologia , Microglia/imunologia , Axônios/patologia , Barreira Hematoencefálica/lesões , Encéfalo/imunologia , Quimiocinas/metabolismo , Citocinas/metabolismo , Humanos , Inflamação , Canais Iônicos/metabolismo , Oligodendroglia/patologia , Espécies Reativas de Oxigênio/metabolismoRESUMO
Epitope H contains an O-linked N-acetylglucosamine (O-GlcNAc) residue in a specific conformation and/or environment recognized by mouse IgM monoclonal antibody H (mabH). Epitope H is present in several types of cells and in several polypeptides outside the CNS. Previous results have shown that in the adult human brains, epitope H is confined mostly to a minority of fibrous astrocytes, and it is greatly upregulated in the reactive astrocytes. Post-translational modification with O-GlcNAc occurs on many proteins involved in several cell processes, such as cell cycle progression, apoptosis, proteasome degradation pathways, and modulation of cellular function in response to nutrition and stress. Hypoxia is one of the major causes of cellular stress. Therefore, in this study, we used the mAbH and the indirect immunoperoxidase method to investigate the expression of epitope H in ependymal cells in brains of persons who died with signs of hypoxic encephalopathy. The results of the present study showed that practically all ependymal cells showed cytoplasmic staining for epitope H in supranuclear cytoplasm in the brain of two premature neonates and in ten infants who died with signs of hypoxic encephalopathy. However, the overwhelming majority of ependymal cells of the nine human embryos taken from legal abortions, ranging from 26 days until 13 weeks of gestational age, and of the ten infants' brains without any sign of hypoxic encephalopathy remained negative. Only occasionally did the ependymal cells show weak cytoplasmic staining in some foci. In addition, the reactive astrocytes in the hypoxic brains showed strong cytoplasmic staining, confirming previous results.
Assuntos
Acetilglucosamina/análise , Epêndima/imunologia , Epitopos/análise , Hipóxia Fetal/imunologia , Hipóxia Encefálica/imunologia , Anticorpos Monoclonais , Astrócitos/imunologia , Citoplasma/imunologia , Epêndima/embriologia , Epêndima/patologia , Hipóxia Fetal/patologia , Técnica Indireta de Fluorescência para Anticorpo , Idade Gestacional , Humanos , Hipóxia Encefálica/embriologia , Hipóxia Encefálica/mortalidade , Hipóxia Encefálica/patologia , Recém-Nascido , Recém-Nascido Prematuro , Regulação para CimaRESUMO
Anti-RhD allo-immunisation has become rare since anti-D prophylaxis was introduced in the seventies; however, it remains the first cause of fetal anemia. It may cause severe fetal complications such as fetal hydrops, cerebral anoxic lesions and fetal death. In the neonatal period, severe jaundices and anemias requiring transfusion or exsanguino-transfusion are still common in case of severe allo-immunisation. Neonatal death and sequellae due to bilirubin encephalopathy have not fully disappeared. Follow-up of pregnancies with maternal allo-immunisation requires identification of the antibody (anti-RhD, anti-Kell and anti-c are the most frequently responsible for fetal complications), dosage and titration. In RhD allo-immunization, feto-maternal incompatibility may be confirmed by non-invasive RHD genotyping of the fetus in maternal blood. In cases at risk for fetal anemia, weekly Doppler assessment of middle cerebral artery peak systolic velocity (MCA-PSV) allows identification of fetal anemia before the occurrence of fetal hydrops. The reference treatment of fetal anemia is in utero fetal transfusion. The risk of fetal loss due to in utero transfusion (IUT) is 3% per procedure. The cumulated risk of fetal loss can thus exceed 10% in case of early occurrence of fetal anemia requiring up to five or six IUTs in a single pregnancy.
Assuntos
Incompatibilidade de Grupos Sanguíneos , Anemia/embriologia , Anemia/terapia , Incompatibilidade de Grupos Sanguíneos/complicações , Incompatibilidade de Grupos Sanguíneos/diagnóstico , Incompatibilidade de Grupos Sanguíneos/terapia , Transfusão de Sangue Intrauterina , Feminino , Morte Fetal/imunologia , Doenças Fetais/terapia , Idade Gestacional , Frequência Cardíaca Fetal , Humanos , Hidropisia Fetal/imunologia , Hipóxia Encefálica/imunologia , Recém-Nascido , Sistema do Grupo Sanguíneo de Kell/imunologia , Kernicterus/imunologia , Artéria Cerebral Média/diagnóstico por imagem , Artéria Cerebral Média/embriologia , Gravidez , Prognóstico , Isoimunização Rh/complicações , Isoimunização Rh/prevenção & controle , Imunoglobulina rho(D)/uso terapêutico , Ultrassonografia Pré-NatalRESUMO
The objective was to explain the discrepancy in the development of hypoxic ischemic brain injury (HIE) in some asphyxiated newborns rather than others. Forty newborns were classified according to their cerebrospinal neuron-specific-enolase (CSF-NSE) levels on their 5th-day of life; group 1 with low-NSE (n = 25). The remaining 15 newborns had high-NSE and were further divided into a group with no HIE (n = 10, group 2) and another with HIE (n = 5, group 3). CSF-NSE, total-hydroperoxide (TH), biological-antioxidant-potentials (BAPs), 12 cytokines and erythropoietin (EPO) were measured. The TH/BAP gave the oxidative-stress-index (OSI). The BAPs of serial dilutions of three types of EPO were tested. CSF-NSE and TH and mean OSIs were higher in group 3. IL-8 and mean BAPs were higher in group 2 than in group 1. EPO was less detected in group 3. Serial EPO dilutions correlated with their BAPs. Compensatory antioxidants and IL-8 elevation could be protective of perinatal asphyxic brain injury. Antioxidative effect of EPO could be neuroprotective.
Assuntos
Antioxidantes/metabolismo , Asfixia Neonatal/complicações , Hipóxia Encefálica/prevenção & controle , Interleucina-8/líquido cefalorraquidiano , Estresse Oxidativo , Assialoglicoproteínas/metabolismo , Asfixia Neonatal/líquido cefalorraquidiano , Asfixia Neonatal/imunologia , Eritropoetina/análogos & derivados , Eritropoetina/líquido cefalorraquidiano , Eritropoetina/metabolismo , Feminino , Humanos , Peróxido de Hidrogênio/líquido cefalorraquidiano , Hipóxia Encefálica/líquido cefalorraquidiano , Hipóxia Encefálica/etiologia , Hipóxia Encefálica/imunologia , Recém-Nascido , Masculino , Fosfopiruvato Hidratase/líquido cefalorraquidiano , Proteínas Recombinantes , Regulação para CimaRESUMO
Perinatal hypoxic-ischaemic brain damage is an important cause of neonatal death and permanent neurological impairment. Therapeutic hypothermia may reduce the development of brain damage after hypoxia. Whether to use room-air or 100% oxygen for resuscitation of the asphyxiated neonate is still debated, and there is little knowledge about the combined effects of therapeutic hypothermia and room air resuscitation. We used human NT2-N neurons to test whether oxygen level during reoxygenation would influence the protective effect of hypothermia. Oxygen-glucose deprived (OGD) human NT2-N neurons were exposed to 20 min of low (1%), medium (21%) or high (95%) oxygen concentrations immediately after hypoxia, followed by 20.5 h of hypothermia (33 degrees C) or normothermia (37 degrees C). Cell viability was determined by a methyltetrazolium assay (MTT), cellular energy failure by hypoxanthine release to supernatant, and inflammatory response by the release of IL-8 (Interleukin-8), bFGF (basic fibroblast growth factor), IP-10 (interferon-inducible protein-10) and MCP-1 (monocyte chemotactic protein-1) to supernatant. Post-hypoxic hypothermia resulted in significantly higher MTT cleavage (average 27% of control (SD 11%) vs 24% (SD 12%), p=0.005). Hypoxanthine release was increased both immediately after hypoxia and 21 h later, however less in hypothermic (median increase 2.0 mumol/L, IQR 1.2-3.2) compared to normothermic cells (2.7 mumol/L, IQR 2.1-4.1, p<0.05). All four inflammatory markers increased after hypoxia but not differently between normothermic and hypothermic cells. Oxygen level had no significant effect on cell viability, inflammatory markers or energy status, irrespective of temperature level. We conclude that hypothermia protects isolated neurons after in vitro hypoxia, and that this protection is not affected by hyperoxic, normoxic or hypoxic reoxygenation.
Assuntos
Sobrevivência Celular , Hipotermia Induzida , Hipóxia Encefálica/terapia , Neurônios/fisiologia , Oxigênio/metabolismo , Linhagem Celular , Quimiocina CCL2/metabolismo , Quimiocina CXCL10/metabolismo , Fator 2 de Crescimento de Fibroblastos/metabolismo , Glucose/deficiência , Humanos , Hipoxantina/metabolismo , Hipóxia/imunologia , Hipóxia/terapia , Hipóxia Encefálica/imunologia , Interleucina-8/metabolismo , Neurônios/imunologia , TemperaturaRESUMO
BACKGROUND: The long-term neurological prognosis of severe fetal anemia is usually considered favorable, especially when fetal hydrops regresses after successful in utero transfusion. CASES: We report two cases of prenatally diagnosed fetal cerebral anoxic lesions associated with severe fetal anemia despite appropriate and successful treatment by in utero transfusion. The two pregnancies were terminated. CONCLUSION: Profound fetal anemia may cause anoxic lesions of the fetal brain that may be diagnosed prenatally. If new onset ventriculomegaly is observed on ultrasonography after in utero transfusion for severe fetal anemia, anoxic lesions could be suspected.
Assuntos
Anemia/imunologia , Doenças Fetais/imunologia , Hipóxia Encefálica/imunologia , Sistema do Grupo Sanguíneo de Kell/imunologia , Isoimunização Rh/complicações , Adulto , Anemia/sangue , Anemia/diagnóstico por imagem , Feminino , Doenças Fetais/sangue , Doenças Fetais/diagnóstico por imagem , Humanos , Hipóxia Encefálica/sangue , Hipóxia Encefálica/diagnóstico por imagem , Gravidez , Isoimunização Rh/diagnóstico por imagem , Ultrassonografia Pré-NatalRESUMO
Microglial cells are endowed with different potassium ion channels but their expression and specific functions have remained to be fully clarified. This study has shown Kv1.2 expression in the amoeboid microglia in the rat brain between 1 (P1) and 10 (P10) days of age. Kv1.2 expression was localized in the ramified microglia at P14 and was hardly detected at P21. In postnatal rats exposed to hypoxia, Kv1.2 immunoreactivity in microglia was markedly enhanced. Quantitative RT-PCR analysis confirmed Kv1.2 mRNA expression in microglial cells in vitro. It was further shown that Kv1.2 and protein expression coupled with that of interleukin 1beta (IL-1beta) and tumor necrosis factor-alpha (TNF-alpha) was significantly increased when the cells were subjected to hypoxia. The same increase was observed in cells exposed to adenosine 5'-triphosphate (ATP) and lipopolysaccharide (LPS). Concomitantly, the intracellular potassium concentration decreased significantly. Blockade of Kv1.2 channel with rTityustoxin-Kalpha (TsTx) resulted in partial recovery of intracellular potassium concentration accompanied by a reduced expression of IL-1beta and TNF-alpha mRNA and protein expression and intracellular reactive oxygen species (ROS) production. We conclude that Kv1.2 in microglia modulates IL-1beta and TNF-alpha expression and ROS production probably by regulating the intracellular potassium concentration.