RESUMO
Radiation workers in medical diagnostic departments are occupationally exposed to long-term low-dose ionizing radiation, which may cause radiation-induced side effects. This study investigated subtypes of peripheral blood lymphocytes and immunoglobulin levels in workers who were occupationally exposed to X-ray radiation at the Department of Radiology. Seventeen radiology workers received low levels of ionizing radiation as the study group and 18 individuals who were not exposed to radiation were included as the control group. The percentage of lymphocyte subtypes (CD4+, CD8+ and CD4+/CD8+) and serum levels of immunoglobulins (IgA, IgG and IgM) were measured using peripheral blood samples. Considering all lymphocyte subtypes and serum levels of IgA, IgG and IgM, there was no significant difference between the study and control groups (P > 0.05). There were no significant differences in all mentioned parameters regarding gender (P > 0.05). For the length of employment period, there was a significant difference concerning CD4+/CD8+ (P < 0.05). The findings showed that exposure to low levels of ionizing radiation does not affect the immune system of workers in diagnostic radiology dose level. Because of relatively small samples of workers, it is suggested that these factors be investigated on larger samples of radiology workers.
Assuntos
Imunidade Celular/efeitos da radiação , Imunidade Humoral/efeitos da radiação , Exposição Ocupacional , Adulto , Feminino , Humanos , Imunoglobulinas/sangue , Linfócitos/efeitos da radiação , Masculino , Pessoa de Meia-Idade , Doses de Radiação , Radiologia , Raios X , Adulto JovemRESUMO
Worldwide mobile telephone and microwave use have resulted in an increasing presence of extremely low-frequency electromagnetic field radiations (ELF-EMFs) in ecosystems. ELF-EMFs have been associated with altered physiological processes that can adversely affect exposed organisms. In this study, Trichoplusia ni Hübner larvae were exposed for 24, 48, or 72 h to ELF-EMFs (60 Hz and 2.0 mT) to assess effects on immune response parameters and fertility. Trichoplusia ni life cycle and fertility were not affected by 24-h exposure. However, the number of apoptotic-like cells and cellular immune response significantly increased (P < 0.01) after 72-h exposure (2- and 1.1-fold, respectively), whereas hemolymph total protein and hemocyte cells were reduced (P < 0.01; 16 and 50%, respectively) after 48-h exposure. Hemocyte cell type analysis resulted in significantly (P < 0.01) higher granulocytes number in the unexposed (2-fold increase) and oenocytoids in the 72-h-exposed larvae (28.6-fold increase). Quantitative retrotranscription (RT-qPCR) showed that after 72-h ELF-EMF exposure, the antimicrobial peptides cecropin, lysozyme, gallerimycin, and pgrp were downregulated by 24,866.0, 2.69-, 119.1-, and 1.45-fold, respectively, whereas attacin and defensin were upregulated by 1.59- and 1.85-fold, respectively. The effect of ELF-EMFs on the T. ni larvae immune response and their potential impact on its physiology and susceptibility to pathogens are discussed. This information may provide new insight of ELF-EMFs on other pest species, as well as for the preservation of ecologically important species.
Assuntos
Campos Eletromagnéticos , Fertilidade/efeitos da radiação , Imunidade Celular/efeitos da radiação , Imunidade Humoral/efeitos da radiação , Lepidópteros/efeitos da radiação , Animais , Feminino , Larva/crescimento & desenvolvimento , Larva/imunologia , Larva/efeitos da radiação , Lepidópteros/crescimento & desenvolvimento , Lepidópteros/imunologia , Lepidópteros/fisiologia , MasculinoRESUMO
Effects of genetic strain and light intensity on growth performance and carcass characteristics of broilers grown to heavy weights were investigated. The experimental design was a randomized complete block design. Treatment structure was a 2 × 5 factorial arrangement with the main factors being strain (Ross × Ross 308, Ross × Ross 708) and light intensity (25, 10, 5, 2.5, and 0.2 lx) with trial as replicates. In each of the 5 trials, chicks of 2 different strains of the same commercial hatchery were equally and randomly distributed into 10 environmentally controlled rooms (5 rooms/strain) at 1 d of age at 50% RH. Each room was randomly assigned 1 of 5 light intensities from 22 to 56 d of age. Feed and water were provided ad libitum. Birds and feed were weighed on 0, 14, 28, 42, and 56 d of age for growth performance. Humoral immune response was determined on d 28, whereas ocular and blood samples were performed on d 42 and 55, respectively. On d 56, 20 (10 males and 10 females) birds/strain from each room were processed to determine weights and yields. Genetic strain was significant (P ≤ 0.05) for most of the examined variables, where Ross × Ross 308 had better growth performance and meat yield in comparison with Ross × Ross 708. Although, there was no main effect of light intensity on growth performance and meat yield, results indicated that birds under 10 and 5 lx intensities showed slightly better growth performance and meat yield compared with birds under 25, 2.5, and 0.2 lx in both strains. There was no effect of strain and light intensity on ocular indices, immune response, plasma corticosterone levels, and mortality. This study shows the positive influence on profits to commercial poultry facilities that are using a low lighting environment to reduce energy cost, optimize feed conversion, and maximize production without compromising the welfare of the broilers.
Assuntos
Criação de Animais Domésticos/métodos , Galinhas/fisiologia , Iluminação , Carne/análise , Animais , Análise Química do Sangue/veterinária , Galinhas/classificação , Galinhas/genética , Galinhas/crescimento & desenvolvimento , Galinhas/imunologia , Olho/crescimento & desenvolvimento , Olho/efeitos da radiação , Feminino , Imunidade Humoral/efeitos da radiação , Masculino , Carne/efeitos da radiação , Fenômenos Fisiológicos Oculares/efeitos da radiação , Distribuição AleatóriaRESUMO
Our previous study suggested that green light or blue light would enhance the broiler immune response; this study was conducted to evaluate whether a combination of green and blue monochromatic light would result in improved immune response. A total of 192 Arbor Acre male broilers were exposed to white light, red light, green light, and blue light from 0 to 26 days. From 27 to 49 days, half of the broilers in green light and blue light were switched to blue light (G-B) and green light (B-G), respectively. The levels of anti-Newcastle disease virus (NDV) and anti-bovine serum albumin (BSA) IgG in G-B group were elevated by 11.9-40.3% and 17.4-48.7%, respectively, compared to single monochromatic lights (P<0.05). Moreover, the proliferation of peripheral blood T and B lymphocytes and the IL-2 concentration in the G-B groups increased by 10.4-36.2%, 10.0-50.0% and 24.7-60.3% (P<0.05), respectively, compared with the single monochromatic light groups. However, the serum TNF-α concentration in the G-B group was reduced by 3.64-40.5% compared to other groups, and no significant difference was found between the G-B and B-G groups in any type of detection index at the end of the experiment. These results suggested that the combination of G-B and B-G monochromatic light could effectively enhance the antibody titer, the proliferation index of lymphocytes and alleviate the stress response in broilers. Therefore, the combination of green and blue monochromatic light can improve the immune function of broilers.
Assuntos
Sistema Imunitário/efeitos da radiação , Luz , Animais , Linfócitos B/citologia , Linfócitos B/imunologia , Proliferação de Células/efeitos da radiação , Galinhas , Imunidade Humoral/efeitos da radiação , Imunoglobulina G/imunologia , Imunoglobulina G/metabolismo , Interleucina-2/imunologia , Masculino , Vírus da Doença de Newcastle/imunologia , Linfócitos T/citologia , Linfócitos T/imunologia , Fatores de Tempo , Fator de Necrose Tumoral alfa/metabolismoRESUMO
The ability of post-radiation (4 Gy) bystander chemosignals (the volatile components of mouse urine) to distantly modulate the humoral immune response to the sheep red blood cells in the spleen and popliteal lymph nodes of intact recipients has been investigated. It was shown that the exposure of animals to chemosignals before antigen injection resulted in the decrease and increase of the immune response in the spleen and lymph nodes, respectively. When animals were exposed to chemosignals after the antigenic stimulus, an increased immune response was observed in both spleen and lymph nodes. The contribution of radiation-induced bystander signaling in the response of socially organized animals to the effect of ionizing irradiation is discussed.
Assuntos
Efeito Espectador/efeitos da radiação , Raios gama , Imunidade Humoral , Linfonodos/imunologia , Baço/imunologia , Urina , Animais , Células Produtoras de Anticorpos/citologia , Células Produtoras de Anticorpos/efeitos dos fármacos , Células Produtoras de Anticorpos/imunologia , Células Produtoras de Anticorpos/efeitos da radiação , Antígenos/imunologia , Contagem de Células , Eritrócitos/imunologia , Imunidade Humoral/efeitos dos fármacos , Imunidade Humoral/efeitos da radiação , Linfonodos/efeitos dos fármacos , Linfonodos/efeitos da radiação , Masculino , Camundongos , Camundongos Endogâmicos C57BL , Camundongos Endogâmicos CBA , Lesões Experimentais por Radiação/imunologia , Lesões Experimentais por Radiação/urina , Ovinos , Baço/efeitos dos fármacos , Baço/efeitos da radiação , Urina/química , Volatilização , Irradiação Corporal TotalRESUMO
AIM: To investigate the protective effect of purified fraction 1 polysaccharide extracted from Rheum tanguticum RTP1 on irradiation-induced immune damage in mice. METHODS: Kunming mice were randomly divided into five groups: normal group (NC), irradiation control group (IC), RTP1 low dose (200 mg/kg), middle dose (400 mg/kg) and high dose (800 mg/kg) groups. RTP1 was administered by the gastric route for 14 d, mice in the NC and IC groups being given by 0.9% sodium chloride solution in the same way. The mice in all groups except NC group were irradiated with 2.0 Gy6°Co γ-ray on the fourteenth day. Immune indives of non-specific immune function, cellular immunity and humoral immunity were assessed at the 24th hour after radiation. RESULTS: Compared with the IC group, the spleen index, thymus index, rate of carbon clearance, phagocytic function of macrophages, lymphocyte proliferation, hemolysin value of blood serum and NK activity were increased markedly (P < 0.05 or P < 0.05). CONCLUSION: RTP1 has an obvious protective effects on damage in γ-ray radiated mice.
Assuntos
Imunidade Celular/efeitos dos fármacos , Imunidade Humoral/efeitos dos fármacos , Fitoterapia , Extratos Vegetais/uso terapêutico , Polissacarídeos/uso terapêutico , Lesões Experimentais por Radiação/prevenção & controle , Animais , Medicamentos de Ervas Chinesas/uso terapêutico , Feminino , Raios gama , Proteínas Hemolisinas/sangue , Proteínas Hemolisinas/efeitos dos fármacos , Proteínas Hemolisinas/efeitos da radiação , Imunidade Celular/efeitos da radiação , Imunidade Humoral/efeitos da radiação , Células Matadoras Naturais/efeitos dos fármacos , Células Matadoras Naturais/efeitos da radiação , Macrófagos/efeitos dos fármacos , Macrófagos/efeitos da radiação , Masculino , Camundongos , Fagocitose/efeitos dos fármacos , Fagocitose/efeitos da radiação , Extratos Vegetais/administração & dosagem , Polissacarídeos/administração & dosagem , Protetores contra Radiação/uso terapêutico , Distribuição Aleatória , Rheum , Baço/efeitos dos fármacos , Baço/efeitos da radiação , Timo/efeitos dos fármacos , Timo/efeitos da radiaçãoRESUMO
There is now good evidence that the use of electromagnetic millimeter waves the following curative effects: analgesic, normalization of relations or increased formation of neurohumoral substances. The introduction of a therapeutic practice complex biological drugs that trigger, not overwhelming the body auxiliary immunological reaction, based on the activation of the regulation clones of T-lymphocytes and helper functions, is an important step in achieving a qualitatively level of health patients with chronic disease.
Assuntos
Bronquite/sangue , Bronquite/reabilitação , Imunidade Celular , Imunidade Humoral , Minerais/uso terapêutico , Extratos Vegetais/uso terapêutico , Terapia por Radiofrequência , Adolescente , Antígenos CD/sangue , Contagem de Células Sanguíneas , Proteínas Sanguíneas/metabolismo , Bronquite/imunologia , Bronquite/prevenção & controle , Terapia Combinada , Feminino , Humanos , Imunidade Celular/efeitos dos fármacos , Imunidade Celular/efeitos da radiação , Imunidade Humoral/efeitos dos fármacos , Imunidade Humoral/efeitos da radiação , Imunoglobulinas/sangue , Masculino , Minerais/administração & dosagem , Extratos Vegetais/administração & dosagem , Prevenção SecundáriaRESUMO
BACKGROUND: Iran is considered as one of the countries which are located in high radiation area. Ramssar contains natural radioactive sources (Uranium-238) that can produce Radium-226 with high solubility and Radon gas with ability to fuse in dirt, soil, and water in its decay chain. Radioactive rays can produce free radicals with a short life time but high energy, which lead to changes structures of protein bindings, hormones activity, lipids oxidation and DNA breakage which can induce autoimmune diseases and other disorders. The aim of this study was to evaluate effects of natural radiation on innate and humoral immune response of those exposed to natural radioactive rays compared to control group. METHODS: Subjects from high and low natural radiation areas were included in the study. Neutrophil chemotaxis, Nitro-Blue Tetrazolium (NBT), and antioxidant effects were evaluated for effects of radioactivity on free radicals and innate immunity. Also Cytokines (IL-2, IL-4) levels for humoral immune system of the subjects were measured. RESULTS: Total plasma antioxidant level and potency of respiratory burst in individuals who lived in area with high levels of natural radiation decreased significantly compared with, control subjects who lived in the other areas of Ramssar with normal radiation. The level of IL-4 increased in individuals who lived in area with high levels of natural radiation, which could lead to Th2 pattern of immune response whether neutrophils chemotaxis in the groups living in the area with high level of natural radiation didn't change significantly when compared to control. CONCLUSION: Neutrophil respiratory burst activities suggested that NADPH probably reduced due to different reasons including decrease in NADPH oxidase and other enzymes, and mutation of the genes or production of oxidant substances. Neutrophil chemotaxis reduced but this reduction was not statistically significant.
Assuntos
Radiação de Fundo , Imunidade Humoral/efeitos da radiação , Imunidade Inata/efeitos da radiação , Fagócitos/efeitos da radiação , Adulto , Antioxidantes/metabolismo , Estudos de Casos e Controles , Citocinas/sangue , Feminino , Humanos , Irã (Geográfico) , Masculino , Pessoa de Meia-IdadeRESUMO
UNLABELLED: The aim of this study is to determine humoral immune status in Ukrainian children with clinical symptoms of irritable bowel syndrome 23 years after the Chernobyl disaster. METHOD AND MATERIAL: The test population consisted of 95 participants: 75 rural patients aged 4-18, who lived in a contaminated area exposed to natural environmental radiation (falling under three groups) and 20 healthy urban participants from Kiev aged 5-15 as a control group. Internal radiation activity has been measured by gamma-ray spectrometry. B-lymphocytes population was analyzed with monoclonal antibody against antigen CD22(+). Serum immunoglobulins were evaluated by enzyme-linked immunosorbent assay (ELISA) method. p < 0.05 was considered significant. RESULT: The percentage of CD22(+) in study groups is increased significantly in comparison to control group at p < 0.05. Reduced serum immunoglobulins levels have developed in the majority of the participants. CONCLUSION: Humoral immune status of study groups with clinical symptom of irritable bowel syndrome residing in a contaminated area has changed.
Assuntos
Radioisótopos de Césio/toxicidade , Acidente Nuclear de Chernobyl , Imunidade Humoral/efeitos da radiação , Síndrome do Intestino Irritável/etiologia , Síndrome do Intestino Irritável/imunologia , Adolescente , Linfócitos B/metabolismo , Criança , Pré-Escolar , Ensaio de Imunoadsorção Enzimática , Humanos , Imunoglobulina A/sangue , Imunoglobulina G/sangue , Imunoglobulina M/sangue , Modelos Lineares , Linfócitos T/metabolismo , Ucrânia/epidemiologiaRESUMO
The obtained data testify about immunmodulating properties of the given medicine. Influence of "Be phytomedicine on organism, which undergoes dust-radiation factor leads to positive dynamics in humoral and cellular parts of immunity, also to increasing immunological reactivity of an organism.
Assuntos
Contaminação Radioativa do Ar/efeitos adversos , Betula/química , Poeira , Imunidade Celular , Imunidade Humoral , Triterpenos/farmacologia , Animais , Imunidade Celular/efeitos dos fármacos , Imunidade Celular/efeitos da radiação , Imunidade Humoral/efeitos dos fármacos , Imunidade Humoral/efeitos da radiação , Ratos , Triterpenos/químicaRESUMO
This article presents the data about state of health and immunity in veterans of extra risk divisions. The increased morbidity and immunity infringement in the remote terms after nuclear tests, and also while liquidation of consequences of radiating failures on nuclear submarines are shown. Changes of humoral factors of nonspecific protection, concentration of immunoglobulinums, in blood whey, a sensitization of lymphocytes to respiratory viruses, humoral and cellular autoimmune shifts are registered. Some of the revealed changes (complement, lysozyme, concentration of immunoglobulinums) are a consequence of advanced age and accompanying diseases in the people surveyed, and others (autoimmune shifts, a sensitization to respiratory viruses) can be connected with carrying out of tests of the nuclear weapon. Some of immunological changes are apparently a consequence of joined actions of radiating and not radiating factors. Among the last ones stress plays the essential role. For the characteristic of a state of health in 20-40 years after carrying out nuclear tests and possible radiating influence the estimation of autoimmune changes has a great value. The important role of such changes in morbidity of veterans of extra risk divisions is shown.
Assuntos
Autoimunidade/efeitos da radiação , Sistema Imunitário , Imunidade Celular/efeitos da radiação , Imunidade Humoral/efeitos da radiação , Imunocompetência/efeitos da radiação , Militares , Adulto , Síndrome de Adaptação Geral/imunologia , Síndrome de Adaptação Geral/fisiopatologia , Síndrome de Adaptação Geral/psicologia , Disparidades nos Níveis de Saúde , Humanos , Sistema Imunitário/fisiopatologia , Sistema Imunitário/efeitos da radiação , Masculino , Pessoa de Meia-Idade , Militares/psicologia , Militares/estatística & dados numéricos , Liberação Nociva de Radioativos/psicologia , Liberação Nociva de Radioativos/estatística & dados numéricos , Saúde Radiológica , Estresse Psicológico/fisiopatologia , Saúde dos VeteranosRESUMO
The comparative analysis of demographic, morphological and physiological processes in mouselike rodents in pollution zones (90Sr + 90Y, 137Cs) on East-Ural radioactive track (EURT) and (Cu + Cd + Pb + Zn + SO2) on a site near copper-smelting factory is carried out. The direct (not mediated) defeat of animals by an irradiation leads to inherited adaptation (density preservation, tolerance increase to pollution, migration decrease and so forth). The mediated defeat of animals at pollution by metals influences animals as a result of degradation of a vegetative cover, reducing a forage reserve, shelters and reproduction places. Population is decreasing, migration is increasing. Hence, population reacts onto direct defeat of animals or on inhabitancy locuses degradation, id est unspecifically, without dependence from the physical and chemical nature of pollution.
Assuntos
Adaptação Fisiológica , Monitoramento Ambiental/métodos , Metais Pesados/toxicidade , Muridae/fisiologia , Poluentes Radioativos/toxicidade , Adaptação Fisiológica/efeitos dos fármacos , Adaptação Fisiológica/efeitos da radiação , Migração Animal/efeitos dos fármacos , Migração Animal/efeitos da radiação , Animais , Peso Corporal/efeitos dos fármacos , Peso Corporal/efeitos da radiação , Aberrações Cromossômicas/efeitos dos fármacos , Aberrações Cromossômicas/efeitos da radiação , Imunidade Celular/efeitos dos fármacos , Imunidade Celular/efeitos da radiação , Imunidade Humoral/efeitos dos fármacos , Imunidade Humoral/efeitos da radiação , Muridae/crescimento & desenvolvimento , Muridae/imunologia , Tamanho do Órgão/efeitos dos fármacos , Tamanho do Órgão/efeitos da radiação , Dinâmica Populacional , Monitoramento de Radiação/métodos , Traçadores Radioativos , SibériaRESUMO
Duchenne muscular dystrophy is a fatal, genetic disorder in which dystrophin-deficient muscle progressively degenerates, for which dystrophin gene transfer could provide effective treatment. The host immune response to dystrophin, however, is an obstacle to therapeutic gene expression. Understanding the dystrophin-induced host immune response will facilitate the discovery of strategies to prolong expression of recombinant dystrophin in dystrophic muscle. Using whole-body irradiation of the dystrophic mdx mouse before gene transfer, we temporally removed the immune system; a 600 rad dose removed peripheral immune cells, which were restored by self-reconstitution, and a 900 rad dose removed central and peripheral immune cells, which were restored by adoptive transfer of bone marrow from a syngeneic, dystrophin-normal donor. The anti-dystrophin humoral response was delayed and dystrophin expression was partially preserved in irradiated, vector-treated mice. Nonirradiated, vector-treated control mice lost muscle dystrophin expression completely, had an earlier anti-dystrophin humoral response and demonstrated muscle fibers focally surrounded with T cells. We conclude that dystrophin gene transfer induced anti-dystrophin humoral immunity and cell-mediated responses that were significantly diminished and delayed by temporal removal of the host central or peripheral immune cells. Furthermore, manipulation of central immunity altered the pattern of regulatory T cells in muscle.
Assuntos
Distrofina/genética , Imunidade Humoral/efeitos da radiação , Distrofia Muscular de Duchenne/imunologia , Irradiação Corporal Total , Animais , DNA Complementar/administração & dosagem , Distrofina/imunologia , Técnicas de Transferência de Genes , Vetores Genéticos , Camundongos , Camundongos Endogâmicos mdx , Linfócitos T Reguladores/imunologia , Linfócitos T Reguladores/efeitos da radiaçãoRESUMO
The effects of acute exposure to low- and high-dose radiation on the quantitative and functional parameters of the immune system were analyzed. C57BL/6 mice were irradiated with different doses of γ radiation (0.01, 0.05, 0.1, 0.5 and 2 Gy) and splenocytes were isolated at various times. Alterations in the distribution and surviving fraction of splenocyte subsets such as CD4(+) and CD8(+) T lymphocytes, regulatory T cells (Treg), natural killer (NK) cells, dendritic cells (DCs) and B lymphocytes were analyzed by flow cytometry. Apoptosis frequency was quantified by the TdT-mediated dUTP-biotin nick end labeling (TUNEL) method 4 h after irradiation. Cytokine expression was investigated by real-time reverse transcription-polymerase chain reaction (RT-PCR). Low doses decreased apoptosis in the splenocyte subpopulations studied most prominently in NK cells and DCs. Exposure to 2 Gy increased apoptosis in all splenocyte subpopulations; B cells were the most sensitive and NK cells and DCs the least sensitive. The lowest cell numbers were measured 3 days after irradiation, with minor changes by day 7. CD8(+) and B cells were rather resistant to low doses but were very sensitive to 2 Gy, while NK cells, DCs and Treg cells were much more resistant to high doses. Expression of the T-helper 1 (Th1)- and helper 2 (Th2)-type cytokines decreased after low doses and increased after high doses. Interleukin 6 (IL-6) reacted at early times and IL-10 at later times. IL-5 levels were consistently elevated. These data highlight the differences in the responses of different splenocyte subpopulations to low- and high-dose radiation.
Assuntos
Sistema Imunitário/efeitos da radiação , Animais , Apoptose/efeitos da radiação , Comunicação Celular/efeitos da radiação , Contagem de Células , Sobrevivência Celular/efeitos da radiação , Citocinas/genética , Citocinas/metabolismo , Relação Dose-Resposta à Radiação , Feminino , Regulação da Expressão Gênica/efeitos da radiação , Sistema Imunitário/citologia , Sistema Imunitário/metabolismo , Imunidade Celular/efeitos da radiação , Imunidade Humoral/efeitos da radiação , Cinética , Subpopulações de Linfócitos/citologia , Subpopulações de Linfócitos/metabolismo , Subpopulações de Linfócitos/efeitos da radiação , Camundongos , Camundongos Endogâmicos C57BL , Baço/imunologia , Baço/efeitos da radiação , Irradiação Corporal TotalRESUMO
BACKGROUND: Interventional cardiologists (ICs) are likely to receive high radiation exposure as a result of procedures they undertake. AIMS: To assess the effects of low-dose X-ray radiation exposure on chromosomal damage and on selected indices of cellular and humoral immunity in ICs. METHODS: The study population consisted of 37 ICs and 37 clinical physicians as the control group with similar age, sex and duration of employment, without any work-related exposure to ionizing radiation. Cytogenetic studies were performed by chromosome aberration analysis and immunological studies by flow cytometry, enzyme-linked immunosorbent assay and immunodiffusion techniques. RESULTS: The frequencies of aberrant cells, chromosome breaks and dicentrics plus centric rings were significantly higher in the exposed group compared to the control group (P < 0.05; P < 0.01; P < 0.001, respectively), without positive correlation between the frequency of dicentric and centric ring aberrations and the cumulative doses of the ICs (r = 0.24, not significant). A significant increase was observed in the expression of activation marker CD69 on TCD4(+) stimulated cells in serum immunoglobulin G and interleukin (IL)-2 (P < 0.05) and a significant decrease in serum IL-10 (P < 0.05) in the ICs compared with that of the control group. There was no statistical difference between the two groups in terms of number of white blood cells and lymphocytes, CD3(+), CD4(+) and CD8(+) T cells, CD19(+) and CD16(+) 56(+) cells and concentrations of interferon (IFN)-gamma, IL-4, IL-6 and IL-8 cytokines. CONCLUSIONS: While cytogenetic results show higher chromosomal damage, some immune responses are stimulated or modulated immunologically in ICs.
Assuntos
Cardiologia , Aberrações Cromossômicas/estatística & dados numéricos , Imunidade Celular/efeitos da radiação , Imunidade Humoral/efeitos da radiação , Doenças Profissionais/epidemiologia , Exposição Ocupacional/efeitos adversos , Adulto , Estudos de Casos e Controles , Aberrações Cromossômicas/efeitos da radiação , Análise Citogenética/métodos , Citocinas/metabolismo , Relação Dose-Resposta à Radiação , Ensaio de Imunoadsorção Enzimática , Humanos , Imunodifusão , Imunoglobulinas/sangue , Contagem de Linfócitos , Masculino , Pessoa de Meia-Idade , Doenças Profissionais/imunologia , Radiação Ionizante , Radiologia IntervencionistaRESUMO
BACKGROUND AND AIM: Sixty male Wistar rats, divided into 4 groups, 15 each, were designed as I-control rats, II-rats orally intubated with Nigella sativa oil (1 ml/kg b.wt./day) for 5 days/week, III-whole body gamma irradiated rats with the estimated LD50/30 (4 Gray) and IV-rats daily intubated with Nigella sativa oil then subjected to whole body gamma irradiation, to investigate the radioprotective potential of Nigella crude oil against hemopoietic adverse effects of gamma irradiation. RESULTS: Irradiation resulted in significant reduction in hemolysin antibodies titers and delayed type hypersensitivity reaction of irradiated rats, in addition to significant leukopenia and significant decrease in plasma total protein and globulin concentrations and depletion of lymphoid follicles of spleen and thymus gland. Furthermore, there was a significant increase in malondialdehyde concentration with a significant decrease in plasma glutathione peroxidase, catalase and erythrocyte superoxide dismutase activities were recorded. Oral administration of Nigella sativa oil before irradiation considerably normalized all the above-mentioned criteria; and produced significant regeneration in spleen and thymus lymphoid follicles. CONCLUSION: Our results strongly recommend Nigella sativa oil as a promising natural radioprotective agent against immunosuppressive and oxidative effects of ionizing radiation.
Assuntos
Nigella sativa/química , Óleos de Plantas/uso terapêutico , Lesões Experimentais por Radiação , Protetores contra Radiação/uso terapêutico , Administração Oral , Animais , Antioxidantes/metabolismo , Proteínas Sanguíneas/metabolismo , Células Cultivadas , Relação Dose-Resposta à Radiação , Contagem de Eritrócitos , Eritrócitos/efeitos dos fármacos , Eritrócitos/enzimologia , Eritrócitos/imunologia , Cobaias , Hemólise/efeitos dos fármacos , Hemólise/efeitos da radiação , Tolerância Imunológica/efeitos dos fármacos , Tolerância Imunológica/efeitos da radiação , Imunidade Celular/efeitos dos fármacos , Imunidade Celular/efeitos da radiação , Imunidade Humoral/efeitos dos fármacos , Imunidade Humoral/efeitos da radiação , Contagem de Leucócitos , Leucopenia/sangue , Leucopenia/etiologia , Leucopenia/prevenção & controle , Peroxidação de Lipídeos/efeitos dos fármacos , Peroxidação de Lipídeos/efeitos da radiação , Masculino , Óleos de Plantas/administração & dosagem , Óleos de Plantas/isolamento & purificação , Lesões Experimentais por Radiação/sangue , Lesões Experimentais por Radiação/imunologia , Lesões Experimentais por Radiação/prevenção & controle , Protetores contra Radiação/administração & dosagem , Protetores contra Radiação/isolamento & purificação , Ratos , Ratos Wistar , Sementes/química , Ovinos , Baço/efeitos dos fármacos , Baço/enzimologia , Baço/efeitos da radiação , Timo/efeitos dos fármacos , Timo/enzimologia , Timo/efeitos da radiação , Irradiação Corporal TotalRESUMO
The children--residents of contaminated territories, born from mothers irradiated in their childhood had in remote period of the Chernobyl Disaster an increase in the frequency of disorders connected with immune system activation and depressive types, increase in intensity of free radical processes in blood serum and erythrocytes.