RESUMO
Control of visceral leishmaniasis (VL) depends on proinflammatory Th1 cells that activate infected tissue macrophages to kill resident intracellular parasites. However, proinflammatory cytokines produced by Th1 cells can damage tissues and require tight regulation. Th1 cell IL-10 production is an important cell-autologous mechanism to prevent such damage. However, IL-10-producing Th1 (type 1 regulatory; Tr1) cells can also delay control of parasites and the generation of immunity following drug treatment or vaccination. To identify molecules to target in order to alter the balance between Th1 and Tr1 cells for improved antiparasitic immunity, we compared the molecular and phenotypic profiles of Th1 and Tr1 cells in experimental VL caused by Leishmania donovani infection of C57BL/6J mice. We also identified a shared Tr1 cell protozoan signature by comparing the transcriptional profiles of Tr1 cells from mice with experimental VL and malaria. We identified LAG3 as an important coinhibitory receptor in patients with VL and experimental VL, and we reveal tissue-specific heterogeneity of coinhibitory receptor expression by Tr1 cells. We also discovered a role for the transcription factor Pbx1 in suppressing CD4+ T cell cytokine production. This work provides insights into the development and function of CD4+ T cells during protozoan parasitic infections and identifies key immunoregulatory molecules.
Assuntos
Interleucina-10 , Infecções por Protozoários , Células Th1 , Células Th1/imunologia , Interleucina-10/genética , Interleucina-10/imunologia , Interleucina-10/metabolismo , Linfócitos T Reguladores/imunologia , Camundongos Endogâmicos C57BL , Leishmania donovani , Leishmaniose Visceral/imunologia , Fator de Transcrição 1 de Leucemia de Células Pré-B/metabolismo , Linfócitos T CD4-Positivos/imunologia , Linfócitos T CD4-Positivos/parasitologia , Infecções por Protozoários/imunologia , Humanos , Animais , Camundongos , Proteína do Gene 3 de Ativação de Linfócitos/antagonistas & inibidores , Interferon gama/metabolismo , Ligação Proteica , Regiões Promotoras Genéticas/imunologia , Modelos Animais de DoençasRESUMO
Protozoan parasite infection causes severe diseases in humans and animals, leading to tremendous economic and medical pressure. Natural immunity is the first line of defence against parasitic infection. Currently, the role of natural host immunity in combatting parasitic infection is unclear, so further research on natural host immunity against parasites will provide a theoretical basis for the prevention and treatment of related parasitic diseases. Extracellular traps (ETs) are an important natural mechanism of immunity involving resistance to pathogens. When immune cells such as neutrophils and macrophages are stimulated by external pathogens, they release a fibrous network structure, consisting mainly of DNA and protein, that can capture and kill a variety of extracellular pathogenic microorganisms. In this review, we discuss the relevant recently reported data on ET formation induced by protozoan parasite infection, including the molecular mechanisms involved, and discuss the role of ETs in the occurrence and development of parasitic diseases.
Assuntos
Armadilhas Extracelulares/imunologia , Imunidade Inata/imunologia , Neutrófilos/imunologia , Infecções Protozoárias em Animais/imunologia , Infecções por Protozoários/imunologia , Transdução de Sinais/imunologia , Animais , Armadilhas Extracelulares/parasitologia , Interações Hospedeiro-Parasita/imunologia , Humanos , Leishmania/imunologia , Leishmania/fisiologia , Neutrófilos/parasitologia , Plasmodium/imunologia , Plasmodium/fisiologia , Infecções por Protozoários/parasitologia , Infecções Protozoárias em Animais/parasitologia , Toxoplasma/imunologia , Toxoplasma/fisiologiaRESUMO
The trypanosomatid pathogens Leishmania spp., Trypanosoma cruzi, and Trypanosoma brucei, currently grouped as TriTryps, have evolved through the time to overcome the upfront innate immune response and establish the infection in humans adapting many aspects of the parasite-cell host interaction. Extracellular vesicles (EVs) emerge as critical structures carrying different key molecules from parasites and target cells that interact continuously during infection. Current information regarding the structure and composition of these vesicles provide new insights into the primary role of TriTryps-EVs reviewed in this work. Expanding knowledge about these critical vesicular structures will promote advances in basic sciences and in translational applications controlling pathogenesis in the neglected tropical diseases caused by TriTryps.
Assuntos
Vesículas Extracelulares/imunologia , Leishmania major/imunologia , Infecções por Protozoários/imunologia , Trypanosoma brucei brucei/imunologia , Trypanosoma cruzi/imunologia , Animais , Vesículas Extracelulares/parasitologia , Interações Hospedeiro-Parasita/imunologia , Humanos , Imunidade Inata/imunologia , Infecções por Protozoários/parasitologiaRESUMO
Since the pioneering discoveries, by the Nobel laureates Jules Hoffmann and Bruce Beutler, that Toll and Toll-like receptors can sense pathogenic microorganisms and initiate, in vertebrates and invertebrates, innate immune responses against microbial infections, many other families of pattern recognition receptors (PRRs) have been described. One of such receptor clusters is composed by, if not all, at least several members of the scavenger receptor cysteine-rich (SRCR) superfamily. Many SRCR proteins are plasma membrane receptors of immune cells; however, a small subset consists of secreted receptors that are therefore in circulation. We here describe the first characterization of biological and functional roles of the circulating human protein SSC4D, one of the least scrutinized members of the family. Within leukocyte populations, SSC4D was found to be expressed by monocytes/macrophages, neutrophils, and B cells, but its production was particularly evident in epithelial cells of several organs and tissues, namely, in the kidney, thyroid, lung, placenta, intestinal tract, and liver. Similar to other SRCR proteins, SSC4D shows the capacity of physically binding to different species of bacteria, and this opsonization can increase the phagocytic capacity of monocytes. Importantly, we have uncovered the capacity of SSC4D of binding to several protozoan parasites, a singular feature seldom described for PRRs in general and here demonstrated for the first time for an SRCR family member. Overall, our study is pioneer in assigning a PRR role to SSC4D.
Assuntos
Infecções Bacterianas/imunologia , Infecções por Protozoários/imunologia , Receptores de Reconhecimento de Padrão/imunologia , Receptores Depuradores Classe B/imunologia , Animais , Bactérias , Linhagem Celular , Células Epiteliais/imunologia , Humanos , Leishmania , Leucócitos/imunologia , Neospora , Fagocitose , Plasmodium berghei , Receptores de Reconhecimento de Padrão/química , Receptores de Reconhecimento de Padrão/genética , Proteínas Recombinantes/imunologia , Receptores Depuradores Classe B/química , Receptores Depuradores Classe B/genética , Trypanosoma brucei bruceiRESUMO
Editorial: The Apicomplexa parasite Toxoplasma gondii glides on substrate with a helical path and releases material that forms a trail behind. The helical microtubules (green) periodically compress and relax, acting as spring force by coupling with the myosin motor (red).
Assuntos
Apicomplexa , Interações Hospedeiro-Parasita/imunologia , Infecções por Protozoários , Proteínas de Protozoários/imunologia , Animais , Apicomplexa/imunologia , Apicomplexa/parasitologia , Humanos , Infecções por Protozoários/imunologia , Infecções por Protozoários/parasitologiaRESUMO
PD-1 as an immune checkpoint molecule down-regulates T cell activity during immune responses in order to prevent autoimmune tissue damage. In chronic infections or tumors, lasting antigen-exposure leads to permanent PD-1 expression that can limit immune-mediated clearance of pathogens or degenerated cells. Blocking PD-1 can enhance T cell function; in cancer treatment PD-1 blockade is already used as a successful therapy. However, the role of PD-1 expression and blocking in the context of acute and chronic infections is less defined. Building on its success in cancer therapy leads to the hypothesis that blocking PD-1 in infectious diseases is also beneficial in acute or chronic infections. This review will focus on the role of PD-1 expression in acute and chronic infections with virus, bacteria, and parasites, with a particular focus on recent studies regarding PD-1 blockade in infectious diseases.
Assuntos
Infecções/imunologia , Receptor de Morte Celular Programada 1/fisiologia , Animais , Antígenos Virais/imunologia , Autoantígenos/imunologia , Infecções Bacterianas/tratamento farmacológico , Infecções Bacterianas/imunologia , Humanos , Tolerância Imunológica , Ativação Linfocitária , Subpopulações de Linfócitos/imunologia , Camundongos Transgênicos , Polimorfismo de Nucleotídeo Único , Receptor de Morte Celular Programada 1/antagonistas & inibidores , Receptor de Morte Celular Programada 1/deficiência , Receptor de Morte Celular Programada 1/genética , Infecções por Protozoários/tratamento farmacológico , Infecções por Protozoários/imunologia , Sepse/imunologia , Viroses/tratamento farmacológico , Viroses/imunologiaRESUMO
Inflammasomes are cytosolic complexes that assemble in response to cellular stress or upon sensing microbial molecules, culminating in cytokine processing and an inflammatory form of cell death called pyroptosis. Inflammasomes are usually composed of a sensor molecule, an adaptor protein, and an inflammatory caspase, such as Caspase-1, which cleaves and activates multiple substrates, including Gasdermin-D, pro-IL-1ß, and pro-IL-18. Ultimately, inflammasome activation promotes inflammation and restriction of the microbial infection. In recent years, many studies have addressed the role of inflammasomes during fungal, bacterial, viral, and parasitic diseases, revealing sophisticated aspects of the host-pathogen interaction. In this review, we summarize recent advances on inflammasome activation in response to intracellular parasites, including Leishmania spp., Plasmodium spp., Trypanosoma cruzi, and Toxoplasma gondii.
Assuntos
Interações Hospedeiro-Patógeno/imunologia , Inflamassomos/imunologia , Infecções por Protozoários/imunologia , Animais , Eucariotos/imunologia , Humanos , Leishmaniose/imunologia , Leishmaniose/parasitologia , Malária/imunologia , Malária/parasitologia , Infecções por Protozoários/parasitologia , Pesquisa/tendências , Toxoplasmose/imunologia , Toxoplasmose/parasitologia , Tripanossomíase/imunologia , Tripanossomíase/parasitologiaRESUMO
DNA vaccinations are promising strategies for treating diseases that require cellular immunity (i.e., cancer and protozoan infection). Here, we report on the use of a liposomal nanocarrier (lipid nanoparticles (LNPs)) composed of an SS-cleavable and pH-activated lipidlike material (ssPalm) as an in vivo DNA vaccine. After subcutaneous administration, the LNPs containing an ssPalmE, an ssPalm with vitamin E scaffolds, elicited a higher gene expression activity in comparison with the other LNPs composed of the ssPalms with different hydrophobic scaffolds. Immunization with the ssPalmE-LNPs encapsulating plasmid DNA that encodes ovalbumin (OVA, a model tumor antigen) or profilin (TgPF, a potent antigen of Toxoplasma gondii) induced substantial antitumor or antiprotozoan effects, respectively. Flow cytometry analysis of the cells that had taken up the LNPs in draining lymph nodes (dLNs) showed that the ssPalmE-LNPs were largely taken up by macrophages and a small number of dendritic cells. We found that the transient deletion of CD169+ macrophages, a subpopulation of macrophages that play a key role in cancer immunity, unexpectedly enhanced the activity of the DNA vaccine. These data suggest that the ssPalmE-LNPs are effective DNA vaccine carriers, and a strategy for avoiding their being trapped by CD169+ macrophages will be a promising approach for developing next-generation DNA vaccines.
Assuntos
Lipídeos/química , Nanopartículas/química , Infecções por Protozoários/imunologia , Vacinas de DNA/química , Vacinas de DNA/imunologia , Vitamina E/imunologia , Animais , Vacinas Anticâncer/química , Vacinas Anticâncer/imunologia , DNA/imunologia , Células Dendríticas/imunologia , Feminino , Concentração de Íons de Hidrogênio , Interações Hidrofóbicas e Hidrofílicas , Imunidade Celular/imunologia , Imunização/métodos , Lipossomos/química , Lipossomos/imunologia , Linfonodos/imunologia , Macrófagos/imunologia , Camundongos Endogâmicos BALB C , Camundongos Endogâmicos C57BL , Neoplasias/imunologia , Ovalbumina/imunologia , Plasmídeos/imunologia , Vitamina E/químicaAssuntos
Infecções por Protozoários/tratamento farmacológico , Infecções por Protozoários/imunologia , Infecções por Protozoários/prevenção & controle , Antiprotozoários/uso terapêutico , Interações Hospedeiro-Parasita/imunologia , Humanos , Malária/tratamento farmacológico , Malária/imunologia , Malária/prevenção & controle , Plasmodium , Vacinas Protozoárias , Trypanosoma , Tripanossomíase/tratamento farmacológico , Tripanossomíase/imunologia , Tripanossomíase/prevenção & controleRESUMO
BACKGROUND: Whirling disease (WD), caused by the myxozoan parasite Myxobolus cerebralis, is responsible for high mortalities in rainbow trout hatcheries and natural populations. To elucidate how resistant and susceptible rainbow trout strains respond to early invasion, a well-established model of WD was used to demonstrate the kinetics of local and systemic immune responses in two rainbow trout strains, the susceptible American Trout Lodge (TL) and the more resistant German Hofer strain (HO). METHODS: Parasite load and cellular immune responses were compared across several time points after M. cerebralis exposure to elucidate the kinetics of immune cells in resistant and susceptible rainbow trout in response to early invasion. In the course of the 20 days following exposure, leukocyte kinetics was monitored by flow cytometry in the caudal fin (CF), head kidney (HK) and spleen (SP). For the analysis of the leukocyte composition, cells were stained using a set of monoclonal antibodies with known specificity for distinct subpopulations of rainbow trout leukocytes. RESULTS: Experiments indicated general increases of CF, HK and SP myeloid cells, while decreases of B cells and T cells in the SP and HK were observed at several time points in the TL strain. On the other hand, in the HO strain, increases of T cells were dominant in CF, HK and SP at multiple time points. The differences between HO and TL were most distinct at 2, 4, 12 and 48 hours post-exposure (hpe) as well as at 4 days post-exposure (dpe), with the vast majority of innate immune response cells having higher values in the susceptible TL strain. Alteration of the leukocyte populations with augmented local cellular responses and excessive immune reactions likely lead to subsequent host tissue damage and supports parasite invasion and development in TL. CONCLUSIONS: The findings of this study highlight the significance of effective local and systemic immune reaction and indicate proper activation of T lymphocytes critical for host resistance during M. cerebralis infection. The present study provides insights into the cellular basis of protective immune responses against M. cerebralis and can help us to elucidate the mechanisms underlying the variation in resistance to WD.
Assuntos
Suscetibilidade a Doenças , Doenças dos Peixes/imunologia , Linfócitos/imunologia , Oncorhynchus mykiss/parasitologia , Doenças Parasitárias em Animais/imunologia , Animais , Linfócitos B/imunologia , Modelos Animais de Doenças , Imunidade Celular , Imunidade Inata , Cinética , Myxobolus/imunologia , Carga Parasitária , Infecções por Protozoários/imunologia , Linfócitos T/imunologiaRESUMO
The peritoneal cavity has a microenvironment capable of promoting proliferation, differentiation, and activation of the resident cells and recruitment of blood cells through the capillary network involved in the peritoneum. Among the cells found in the peritoneal cavity, B-1 cells are a particular cell type that contains features that are not very well defined. These cells differ from conventional B lymphocytes (B-2) by phenotypic, functional, and molecular characteristics. B-1 cells can produce natural antibodies, migrate to the inflammatory focus, and have the ability to phagocytose pathogens. However, the role of B-1 cells in immunity against parasites is still not completely understood. Several experimental models have demonstrated that B-1 cells can affect the susceptibility or resistance to parasite infections depending on the model and species. Here, we review the literature to provide information on the peculiarities of B-1 lymphocytes as well as their interaction with parasites.
Assuntos
Subpopulações de Linfócitos B/imunologia , Helmintíase/imunologia , Helmintos/imunologia , Imunidade Humoral/imunologia , Parasitos/imunologia , Cavidade Peritoneal/citologia , Infecções por Protozoários/imunologia , Animais , Citocinas/biossíntese , Citocinas/imunologia , Helmintíase/parasitologia , Humanos , Camundongos , Peritônio/citologia , Peritônio/imunologia , Infecções por Protozoários/parasitologiaRESUMO
Apicomplexans are a diverse and complex group of protozoan pathogens including Toxoplasma gondii, Plasmodium spp., Cryptosporidium spp., Eimeria spp., and Babesia spp. They infect a wide variety of hosts and are a major health threat to humans and other animals. Innate immunity provides early control and also regulates the development of adaptive immune responses important for controlling these pathogens. Innate immune responses also contribute to immunopathology associated with these infections. Natural killer (NK) cells have been for a long time known to be potent first line effector cells in helping control protozoan infection. They provide control by producing IL-12 dependent IFNγ and killing infected cells and parasites via their cytotoxic response. Results from more recent studies indicate that NK cells could provide additional effector functions such as IL-10 and IL-17 and might have diverse roles in immunity to these pathogens. These early studies based their conclusions on the identification of NK cells to be CD3-, CD49b+, NK1.1+, and/or NKp46+ and the common accepted paradigm at that time that NK cells were one of the only lymphoid derived innate immune cells present. New discoveries have lead to major advances in understanding that NK cells are only one of several populations of innate immune cells of lymphoid origin. Common lymphoid progenitor derived innate immune cells are now known as innate lymphoid cells (ILC) and comprise three different groups, group 1, group 2, and group 3 ILC. They are a functionally heterogeneous and plastic cell population and are important effector cells in disease and tissue homeostasis. Very little is known about each of these different types of ILCs in parasitic infection. Therefore, we will review what is known about NK cells in innate immune responses during different protozoan infections. We will discuss what immune responses attributed to NK cells might be reconsidered as ILC1, 2, or 3 population responses. We will then discuss how different ILCs may impact immunopathology and adaptive immune responses to these parasites.
Assuntos
Imunidade Adaptativa , Apicomplexa/imunologia , Imunidade Inata , Subpopulações de Linfócitos/imunologia , Subpopulações de Linfócitos/metabolismo , Infecções por Protozoários/imunologia , Infecções por Protozoários/parasitologia , Animais , Biomarcadores , Plasticidade Celular/imunologia , Citocinas/metabolismo , Interações Hospedeiro-Parasita , Humanos , Imunofenotipagem , Mediadores da Inflamação/metabolismo , Plasmodium/imunologiaRESUMO
Adenosine triphosphate (ATP) is released from host cells during parasite infections and acts as a danger signal in the extracellular space by activating plasma membrane purinergic type 2 receptors-P2 receptors. The activation of these receptors has been described as a crucial step in immune cell activation, inflammation and parasite control. The P2X7 receptor is most involved in the activation of host microbicidal mechanisms, including production of reactive oxygen and nitrogen species, phagolysosomal fusion, acidification of parasitophorous vacuoles and release of cytokines and chemokines. The P2X7 receptor also modulates adaptive immune responses in various infectious diseases. Here, we discuss key points from the recent literature regarding P2X7 receptor activation during intracellular parasite infections.
Assuntos
Infecções Bacterianas/imunologia , Infecções por Protozoários/imunologia , Receptores Purinérgicos P2X7/imunologia , Viroses/imunologia , Animais , Humanos , ImunomodulaçãoRESUMO
In biology, models are experimental systems meant to recreate aspects of diseases or human tissue with the goal of generating inferences and approximations that can contribute to the resolution of specific biological problems. Although there are many models for studying intracellular parasites, their data have produced critical contradictions, especially in immunological assays. Peripheral blood mononuclear cells (PBMCs) represent an attractive tissue source in pharmacogenomics and in molecular and immunologic studies, as these cells are easily collected from patients and can serve as sentinel tissue for monitoring physiological perturbations due to disease. However, these cells are a very sensitive model due to variables such as temperature, type of stimulus and time of collection as part of posterior processes. PBMCs have been used to study Toxoplasma gondii and other apicomplexan parasites. For instance, this model is frequently used in new therapies or vaccines that use peptides or recombinant proteins derived from the parasite. The immune response to T. gondii is highly variable, so it may be necessary to refine this cellular model. This mini review highlights the major approaches in which PBMCs are used as a model of study for T. gondii and other apicomplexan parasites. The variables related to this model have significant implications for data interpretation and conclusions related to host-parasite interaction.
Assuntos
Apicomplexa/crescimento & desenvolvimento , Apicomplexa/imunologia , Interações Hospedeiro-Patógeno , Leucócitos Mononucleares/imunologia , Leucócitos Mononucleares/parasitologia , Modelos Teóricos , Infecções por Protozoários/fisiopatologia , Animais , Pesquisa Biomédica/tendências , Humanos , Infecções por Protozoários/imunologia , Infecções por Protozoários/parasitologiaRESUMO
Innate immunity is an evolutionarily ancient form of host defense that serves to limit infection. The invading microorganisms are detected by the innate immune system through germline-encoded PRRs. Different classes of PRRs, including TLRs and cytoplasmic receptors, recognize distinct microbial components known collectively as PAMPs. Ligation of PAMPs with receptors triggers intracellular signaling cascades, activating defense mechanisms. Despite the fact that Gram-negative bacteria and parasitic protozoa are phylogenetically distant organisms, they express glycoconjugates, namely bacterial LPS and protozoan GPI-anchored glycolipids, which share many structural and functional similarities. By activating/deactivating MAPK signaling and NF-κB, these ligands trigger general pro-/anti-inflammatory responses depending on the related patterns. They also use conservative strategies to subvert cell-autonomous defense systems of specialized immune cells. Signals triggered by Gram-negative bacteria and parasitic protozoa can interfere with host homeostasis and, depending on the type of microorganism, lead to hypersensitivity or silencing of the immune response. Activation of professional immune cells, through a ligand which triggers the opposite effect (antagonist versus agonist) appears to be a promising solution to restoring the immune balance.
Assuntos
Antígenos de Bactérias/imunologia , Antígenos de Protozoários/imunologia , Glicoconjugados/imunologia , Bactérias Gram-Negativas/imunologia , Infecções por Bactérias Gram-Negativas/imunologia , Moléculas com Motivos Associados a Patógenos/imunologia , Infecções por Protozoários/imunologia , Animais , Humanos , Imunidade Inata , Doenças Parasitárias , Receptores de Reconhecimento de Padrão/metabolismo , Transdução de SinaisRESUMO
The parasitic dinoflagellate Hematodinium is considered an important pathogen of economically important marine crustaceans and has been reported from many wild and cultured species. While limited studies have been conducted to reveal the host-parasite interaction in crustaceans, the underlying molecular mechanisms between Hematodinium and its crustacean hosts are scarcely known. We conducted a comprehensive study to investigate the proteomic responses to Hematodinium infection in the hepatopancreas of Portunus trituberculatus using an iTRAQ-based quantitative proteomic technology. A total of 905 identified proteins including 392 differentially expressed proteins (DEPs) were subjected to GO, COG and KEGG-pathway enrichment analysis, with sixteen DEPs further validated by quantitative real-time PCR. Hematodinium parasites resulted in immune-suppressive and adverse effects on affected hosts, thorough inhibition of the important pattern recognition receptors (C-lectin, SR class B, and Toll)-mediated immune responses, regulation of the complement and coagulation pathway, dysregulation of important cell adhesion molecules and extracellular matrix, and imbalance of the cellular redox homeostasis in the hepatopancreas of affected crabs. Moreover, the lysosomes pathway was dysregulated seriously in the hepatopancreas of P. trituberculatus post Hematodinium challenge. The results provided evidences on how the Hematodinium parasite overcame the innate immunity of P. trituberculatus and caused pathological alteration in affected tissues BIOLOGICAL SIGNIFICANCE: The manuscript presented the first iTRAQ-based proteomic study of the host-parasite interaction between an important marine crustacean and the parasitic dinoflagellate Hematodinium. The manuscript reported the key pathways and proteins involved in the host-parasite interactions. The major findings will contribute to the better understanding of the molecular mechanism of the particular host-parasite interaction, as wells as the pathogenic process in susceptible tissues of affected crustacean hosts.
Assuntos
Alveolados/imunologia , Proteínas de Artrópodes/imunologia , Braquiúros/imunologia , Hepatopâncreas , Proteômica , Infecções por Protozoários/imunologia , Animais , Hepatopâncreas/imunologia , Hepatopâncreas/parasitologiaRESUMO
The phylum Cnidaria (sea anemones, corals, hydra, jellyfish) is one the most distantly related animal phyla to humans, and yet cnidarians harbor many of the same cellular pathways involved in innate immunity in mammals. In addition to its role in pathogen recognition, the innate immune system has a role in managing beneficial microbes and supporting mutualistic microbial symbioses. Some corals and sea anemones undergo mutualistic symbioses with photosynthetic algae in the family Symbiodiniaceae. These symbioses can be disrupted by anthropogenic disturbances of ocean environments, which can have devastating consequences for the health of coral reef ecosystems. Several studies of cnidarian-Symbiodiniaceae symbiosis have implicated proteins in the host immune system as playing a role in both symbiont tolerance and loss of symbiosis (i.e., bleaching). In this review, we critically evaluate current knowledge about the role of host immunity in the regulation of symbiosis in cnidarians.
Assuntos
Cnidários/imunologia , Dinoflagellida/fisiologia , Imunidade Inata , Infecções por Protozoários/imunologia , Simbiose , Animais , Interações Hospedeiro-Parasita , Humanos , Tolerância Imunológica , Transdução de SinaisRESUMO
Helminths are ubiquitous and have chronically infected vertebrates throughout their evolution. As such helminths have likely exerted considerable selection pressure on our immune systems. The large size of multicellular helminths and their limited replicative capacity in the host necessarily elicits different host protective mechanisms than the immune response evoked by microbial pathogens such as bacteria, viruses and intracellular parasites. The cellular damage resulting from helminth migration through tissues is a major trigger of the type 2 and regulatory immune responses, which activates wound repair mechanisms that increases tissue tolerance to injury and resistance mechanisms that enhance resistance to further colonization with larval stages. While these wound healing and anti-inflammatory responses may be beneficial to the helminth infected host, they may also compromise the host's ability to mount protective immune responses to microbial pathogens. In this review we will first describe helminth-induced tolerance mechanisms that develop in specific organs including the lung and the intestine, and how adaptive immunity may contribute to these responses through differential activation of T cells in the secondary lymphoid organs. We will then integrate studies that have examined how the immune response is modulated in these specific tissues during coinfection of helminths with viruses, protozoa, and bacteria.
Assuntos
Infecções Bacterianas/imunologia , Helmintíase/imunologia , Interações Hospedeiro-Parasita/imunologia , Ativação Linfocitária , Infecções por Protozoários/imunologia , Linfócitos T/imunologia , Viroses/imunologia , Animais , Infecções Bacterianas/patologia , Suscetibilidade a Doenças , Helmintíase/patologia , Humanos , Infecções por Protozoários/patologia , Linfócitos T/patologia , Viroses/patologiaRESUMO
Guanylate-binding proteins (GBPs) are conserved family of IFN-inducible GTPases that play an important role in the host immunity against bacterial, viral, and protozoan pathogens. GBPs protect the host by associating with intracellular microbes, their vacuolar niche or, in the case of viruses, with their replication complex. This association results in a restriction of the respective pathogen, yet the exact molecular mechanisms of the antimicrobial functions of GBPs are still unclear. Recent work has linked the GBPs with the activation of inflammasomes, multi-protein complexes that assemble upon recognition of pathogen- or host-derived signals and that drive the release of cytokines and host cell death. Here, we will focus on the most recent findings that have started to unravel the manifold restriction mechanism controlled by GBPs in mouse and human cells, and that shed light on the molecular cues that control GBP recruitment to bacterial membranes.