RESUMO
Actinodaphne angustifolia Nees (Family: Lauraceae) is commonly used in folk medicine against urinary disorder and diabetes. The objective of the present study was to evaluate the antioxidant, cytotoxic, thrombolytic, and antidiarrheal activities of carbon tetrachloride (CCl4) fraction of leaves of A. angustifolia (CTFAA) in different experimental models. Antioxidant activity was evaluated by using qualitative and quantitative assays, while antidiarrheal effects assessed with castor oil-induced diarrheal models in mice. The clot lysis and brine shrimp lethality bioassay were used to investigate the thrombolytic and cytotoxic activities, respectively. CTFAA showed antioxidant effects in all qualitative and quantitative procedures. The fraction produced dose-dependent and significant (P<0.05 and P<0.01) activities in castor oil-induced diarrheal models. Moreover, CTFAA significantly (P<0.05) demonstrated a 15.29% clot lysis effect in the thrombolytic test, and the brine shrimp lethality assay LC50 value was 424.16 µg/ml bioassay. In conclusion, the current study showed CTFAA has significant antidiarrheal effects along with modest antioxidant and thrombolytic effects, and these data warrant further experiment to justify and include CTFAA as a supplement to mitigate the onset of diarrheal and cardiovascular disease.
Assuntos
Antidiarreicos/farmacologia , Antioxidantes/farmacologia , Diarreia/prevenção & controle , Fibrinólise/efeitos dos fármacos , Fibrinolíticos/farmacologia , Lauraceae , Extratos Vegetais/farmacologia , Folhas de Planta , Animais , Antidiarreicos/isolamento & purificação , Antidiarreicos/toxicidade , Antioxidantes/isolamento & purificação , Antioxidantes/toxicidade , Artemia/efeitos dos fármacos , Tetracloreto de Carbono/química , Óleo de Rícino , Defecação/efeitos dos fármacos , Diarreia/induzido quimicamente , Diarreia/fisiopatologia , Modelos Animais de Doenças , Relação Dose-Resposta a Droga , Feminino , Fibrinolíticos/isolamento & purificação , Fibrinolíticos/toxicidade , Humanos , Lauraceae/química , Lauraceae/toxicidade , Dose Letal Mediana , Masculino , Camundongos , Extratos Vegetais/isolamento & purificação , Extratos Vegetais/toxicidade , Folhas de Planta/química , Folhas de Planta/toxicidade , Solventes/químicaRESUMO
The hydro-alcohol extract of the dry leaves of Peumus boldus and boldine, showed abortive and teratogenic action and changes in the blood levels of bilirubin, cholesterol, glucose, alanine aminotransferase (ALT), aspartate aminotransferase (AST) and urea in rats. The long term administration of the extract and boldine did not cause histological modification during a period of 90 days.