RESUMO
Moreau score has been used to differentiate chronic lymphocytic leukemia (CLL) from other mature B-cell neoplasms. However, it showed limitations in Asian patients. Therefore, we conducted a new score system replacing CD5 and CD23 with CD43 and CD180 to evaluate its diagnostic value of CLL. 237 untreated samples diagnosed with mature B-cell neoplasms were collected and were randomly divided into an exploratory and a validation cohort by a 2:1 ratio. The expression of CD5, CD19, CD20, CD23, CD43, CD79b, CD180, CD200, FMC7, and surface immunoglobulin (SmIg) were analyzed among all the samples. A proposed score was developed based on the logistic regression model. The sensitivity and specificity of the proposed score were calculated by ROC curves. CD43/CD180, CD200, FMC7, and CD79b were included in our new CLL score, which showed a sensitivity of 91.8% and a specificity of 83.1%. These results were confirmed in a validation cohort with a sensitivity of 90.5% (p = 0.808) and a specificity of 79.5% (p = 0.639). In CD5 negative or CD23 negative CLL group, the new CLL score displayed improved sensitivity of 79.4% compared to Moreau score and CLLflow score (41.2% and 47.1%, respectively). In atypical CLL group, the new CLL score showed improved sensitivity of 84.2% compared to Moreau score and CLLflow score (61.4% and 64.9%, respectively). This proposed atypical CLL score helped to offer an accurate differentiation of CLL from non-CLL together with morphological and molecular methods, particularly in Chinese patients with atypical immunophenotype.
Assuntos
Antígenos CD/análise , Biomarcadores Tumorais/análise , Leucemia Linfocítica Crônica de Células B/diagnóstico , Leucossialina/análise , Antígenos CD19/análise , Antígenos CD20/análise , Antígenos CD5/análise , Antígenos CD79/análise , Diagnóstico Diferencial , Citometria de Fluxo/métodos , Glicoproteínas/análise , Humanos , Imunofenotipagem , Leucemia Linfocítica Crônica de Células B/metabolismo , Modelos Logísticos , Linfoma de Células B/diagnóstico , Linfoma de Células B/metabolismo , Curva ROC , Receptores de Antígenos de Linfócitos B/análise , Receptores de IgE/análise , Sensibilidade e EspecificidadeRESUMO
Objectives: CD43 can be useful in routine flow cytometry. We conducted a systematic review aiming to describe when CD43 is used by flow cytometry in malignant hematology and to determine its value in these settings. Methods: Systematic review of MEDLINE (search 'CD43' AND 'flow cytometry,' starting in 2010). Results: Twenty-one of 103 entries retrieved were included in this systematic review. CD43 is used in three settings: 1) in the classification of mature B cell lymphoproliferative disorders, 2) as part of a strategy to quantify residual disease in chronic lymphocytic leukemia (CLL) and 3) to help classify CD10-positive B cell populations. In this section, the published data is summarized, the clinical usefulness in each of these settings is evaluated and illustrative cases are shown. Conclusion: CD43 has a growing role in the diagnosis and management of B cell malignancies; it has become essential for the classification of B cell lymphoproliferative disorders and may be of help in the differential diagnosis of CD10-positive lymphomas by FC. It is also required for optimal quantification of CLL residual disease, which will soon be used to guide therapeutic decisions.
Assuntos
Neoplasias Hematológicas/diagnóstico , Leucemia Linfocítica Crônica de Células B/diagnóstico , Leucossialina/análise , Transtornos Linfoproliferativos/diagnóstico , Animais , Linfócitos B/patologia , Citometria de Fluxo/métodos , Humanos , Neoplasia Residual/diagnósticoRESUMO
OBJECTIVE: Atypical chronic lymphocytic leukemia (CLL) is most frequently confused with mantle cell lymphoma (MCL). Several markers may contribute to the diagnosis of CLL. However, there is no consensus on which markers are needed to be used in flow cytometry for the diagnosis of CLL. The aim of the present study was to investigate the role of CD43 and CD200 markers in the differential diagnosis between CLL and MCL. MATERIALS AND METHODS: To address this issue, 339 consecutive patients with CLL and MCL were included in the flow cytometry lymphoproliferative disease panel for evaluation of CD43 and CD200 expressions, but not in the Matutes scoring system. RESULTS: CD200 was expressed in 97.3% of atypical CLL cases, whereas it was dimly expressed in only 6.1% of MCL cases. CD43 expression was 95.7% in atypical CLL cases. In the MCL cases, its expression rate was 39.4%. CONCLUSION: CD43 and CD200 were found to be more valuable markers than CD22, CD79b, and FMC7. CD43 and CD200 could also be considered as definitive markers in atypical CLL patients, for whom the Matutes scoring system remains ineffective.
Assuntos
Antígenos CD/análise , Leucemia Linfocítica Crônica de Células B/diagnóstico , Leucossialina/análise , Linfoma de Célula do Manto/diagnóstico , Adulto , Idoso , Idoso de 80 Anos ou mais , Biomarcadores Tumorais/análise , Diagnóstico Diferencial , Feminino , Citometria de Fluxo , Humanos , Imunofenotipagem , Masculino , Pessoa de Meia-IdadeAssuntos
Linfoma não Hodgkin/diagnóstico , Linfoma não Hodgkin/patologia , Vulva/patologia , Neoplasias Vulvares/diagnóstico , Neoplasias Vulvares/patologia , Antígenos CD20/análise , Antineoplásicos/administração & dosagem , Cloridrato de Bendamustina/administração & dosagem , Biomarcadores Tumorais/análise , Biópsia , Feminino , Histocitoquímica , Humanos , Imuno-Histoquímica , Leucossialina/análise , Linfoma não Hodgkin/diagnóstico por imagem , Linfoma não Hodgkin/tratamento farmacológico , Imageamento por Ressonância Magnética , Microscopia , Pessoa de Meia-Idade , Rituximab/administração & dosagem , Resultado do Tratamento , Neoplasias Vulvares/diagnóstico por imagem , Neoplasias Vulvares/tratamento farmacológicoAssuntos
Perna (Membro) , Papulose Linfomatoide/patologia , Neoplasias Cutâneas/patologia , Idoso , Feminino , Humanos , Antígeno Ki-1/análise , Leucossialina/análise , Papulose Linfomatoide/metabolismo , Papulose Linfomatoide/cirurgia , Recidiva Local de Neoplasia , Pele/química , Pele/patologia , Neoplasias Cutâneas/metabolismo , Neoplasias Cutâneas/cirurgiaRESUMO
Granulocyte-monocyte progenitors (GMPs) and monocyte-dendritic cell progenitors (MDPs) produce monocytes during homeostasis and in response to increased demand during infection. Both progenitor populations are thought to derive from common myeloid progenitors (CMPs), and a hierarchical relationship (CMP-GMP-MDP-monocyte) is presumed to underlie monocyte differentiation. Here, however, we demonstrate that mouse MDPs arose from CMPs independently of GMPs, and that GMPs and MDPs produced monocytes via similar but distinct monocyte-committed progenitors. GMPs and MDPs yielded classical (Ly6Chi) monocytes with gene expression signatures that were defined by their origins and impacted their function. GMPs produced a subset of "neutrophil-like" monocytes, whereas MDPs gave rise to a subset of monocytes that yielded monocyte-derived dendritic cells. GMPs and MDPs were also independently mobilized to produce specific combinations of myeloid cell types following the injection of microbial components. Thus, the balance of GMP and MDP differentiation shapes the myeloid cell repertoire during homeostasis and following infection.
Assuntos
Células Dendríticas/fisiologia , Células Precursoras de Granulócitos/fisiologia , Monócitos/fisiologia , Células Progenitoras Mieloides/fisiologia , Animais , Antígenos Ly/análise , Diferenciação Celular , Leucossialina/análise , Camundongos , Análise de Sequência de RNA , TranscriptomaRESUMO
We encountered a patient with infantile nephrotic syndrome associated with a dense interstitial inflammatory infiltrate and prominent extramedullary hematopoiesis. Immunohistochemical analysis revealed numerous terminal deoxynucleotidyl transferase (TdT)-positive cells, which may raise concern for lymphoblastic lymphoma. Thus, we further characterized a group of pediatric kidneys with inflammation. TdT-positive nuclei were quantitated, and dual immunostains for TdT/CD79a, TdT/CD3, and TdT/CD43 were performed in a subset of cases; flow cytometry was performed in 1 case. TdT-positive nuclei were present in inflamed pediatric kidneys in 40 of 42 patients. TdT counts (average of 3 maximal high-power fields) ranged from 1 to >200, with a mean of 47. The presence and number of TdT-positive nuclei showed a strong association with younger patient age. Extramedullary hematopoiesis was identified in 11/42 patients, all under the age of 1. The presence of extramedullary hematopoiesis did not correlate with TdT count (P=0.158). Dual immunostaining and flow cytometric analysis in 1 case showed weak expression of B-cell markers and favored normal precursor B cells. Although TdT is a common marker of lymphoblastic lymphoma, we have demonstrated that TdT-positive cells may be part of the inflammatory milieu in infant kidneys. Together with cytologic, architectural, and clinical features, these data can help to avoid misinterpretation of involvement by lymphoblastic lymphoma/leukemia.
Assuntos
DNA Nucleotidilexotransferase/análise , Rim/química , Nefrite/metabolismo , Síndrome Nefrótica/metabolismo , Adolescente , Biomarcadores/análise , Biópsia , Complexo CD3/análise , Antígenos CD79/análise , Criança , Pré-Escolar , Diagnóstico Diferencial , Citometria de Fluxo , Hematopoese Extramedular , Humanos , Imuno-Histoquímica , Lactente , Recém-Nascido , Rim/patologia , Rim/cirurgia , Leucossialina/análise , Masculino , Nefrectomia , Nefrite/diagnóstico , Nefrite/cirurgia , Síndrome Nefrótica/diagnóstico , Síndrome Nefrótica/cirurgia , Leucemia-Linfoma Linfoblástico de Células Precursoras/diagnóstico , Leucemia-Linfoma Linfoblástico de Células Precursoras/metabolismo , Valor Preditivo dos Testes , Adulto JovemRESUMO
Inactivation of the tumor suppressor gene encoding the transcriptional regulator Ikaros (IKZF1) is a hallmark of BCR-ABL1+ precursor B cell acute lymphoblastic leukemia (pre-B ALL). However, the mechanisms by which Ikaros functions as a tumor suppressor in pre-B ALL remain poorly understood. Here, we analyzed a mouse model of BCR-ABL1+ pre-B ALL together with a new model of inducible expression of wild-type Ikaros in IKZF1 mutant human BCR-ABL1+ pre-B ALL. We performed integrated genome-wide chromatin and expression analyses and identified Ikaros target genes in mouse and human BCR-ABL1+ pre-B ALL, revealing novel conserved gene pathways associated with Ikaros tumor suppressor function. Notably, genetic depletion of different Ikaros targets, including CTNND1 and the early hematopoietic cell surface marker CD34, resulted in reduced leukemic growth. Our results suggest that Ikaros mediates tumor suppressor function by enforcing proper developmental stage-specific expression of multiple genes through chromatin compaction at its target genes.
Assuntos
Proteínas de Fusão bcr-abl/análise , Fator de Transcrição Ikaros/fisiologia , Leucemia-Linfoma Linfoblástico de Células Precursoras B/genética , Proteínas Supressoras de Tumor/fisiologia , Animais , Antígenos CD34/análise , Ciclo Celular , Linhagem Celular Tumoral , Regulação Leucêmica da Expressão Gênica , Humanos , Fator de Transcrição Ikaros/genética , Leucossialina/análise , Camundongos , Camundongos Endogâmicos C57BL , Proteínas Proto-Oncogênicas c-kit/genéticaRESUMO
The goal of this study was to identify immunological markers for use in antigen-specific assays that predict long-term survival after renal allograft and distinguish stable-functioning (SP) patients from poorly functioning (PP) patients. For this prospective study, 20 patients were enrolled. Eight SP and six PP patients were enrolled in this study. Serum cytokine/chemokine levels were analyzed by the Luminex multiplex assay. To detect indirect alloreactive T cells, we performed indirect mixed lymphocyte reaction using donor-antigen-pulsed autologous dendritic cells as stimulators. Serum induced protein-10 levels were significantly higher in the serum of PP patients, whereas sCD40L levels were higher in SP patients. The PP patients had significantly higher numbers of donor-specific CD4(+)CD43(high)CD45RO(+) T cells after indirect allostimulation, whereas this cell population was unchanged in SP patients. The donor-specific CD4(+)CD43(high)CD45RO(+) T cells had the effector memory T cell phenotype. Prospectively, we studied whether these cells influence graft outcome and found that their strong proliferation in pre-transplant patients is related to a poorly functioning graft. Indirectly allostimulated CD4(+)CD43(high)CD45RO(+) T cells may not only contribute to chronic allograft nephropathy development but may also have a role in the progression of acute rejection. Thus, these cells may have potential use as immune-monitoring markers in a noninvasive in vitro assay that predicts graft outcome.
Assuntos
Aloenxertos , Linfócitos T CD4-Positivos/imunologia , Rejeição de Enxerto/patologia , Transplante de Rim , Antígenos Comuns de Leucócito/análise , Leucossialina/análise , Subpopulações de Linfócitos T/imunologia , Adulto , Idoso , Biomarcadores/análise , Linfócitos T CD4-Positivos/química , Citocinas/sangue , Feminino , Rejeição de Enxerto/diagnóstico , Humanos , Masculino , Pessoa de Meia-Idade , Estudos Prospectivos , Subpopulações de Linfócitos T/químicaRESUMO
Acute megakaryoblastic leukemia (AMKL) is a relatively common type of acute myeloid leukemia in children. We describe two unusual cases of AMKL that by flow cytometry (FC) lacked expression of any commonly evaluated myeloid antigens. One case presented as a periorbital myeloid sarcoma and clinically was thought to be a solid tumor. In both cases, the leukemic blasts were variably positive for the megakaryocytic marker CD61. Cytogenetics confirmed the presence of the t(1;22) in one case. Cytogenetics and inclusion of megakaryocytic markers in FC panels when evaluating pediatric specimens is critical for appropriate diagnosis for myeloid antigen negative AMKL.
Assuntos
Leucemia Megacarioblástica Aguda/imunologia , Pré-Escolar , Feminino , Humanos , Imunofenotipagem , Lactente , Leucemia Megacarioblástica Aguda/genética , Leucemia Megacarioblástica Aguda/patologia , Leucossialina/análise , Masculino , Translocação GenéticaRESUMO
Major histocompatibility complex class II-deficient (MHC-II KO; Aß(-/-)) mice were used to assess the roles of MHC-II molecules in inducing protective immune responses to vaccination. After vaccination with influenza A/PR8 virus-like particle (VLP) vaccine, in vivo and in vitro vaccine antigen-specific IgG isotype antibodies were not detected in MHC-II KO mice, which is quite different from CD4 T cell-deficient mice that induced vaccine-specific IgG antibodies. The deficiency in MHC-II did not significantly affect the induction of antigen-specific IgM antibody in sera. MHC-II KO mice that were vaccinated with influenza VLP, whole inactivated influenza virus, or live attenuated influenza virus vaccines were not protected against lethal infection with influenza A/PR8 virus. Adoptive transfer of fractionated spleen cells from wild-type mice to MHC-II KO mice indicated that CD43(+) cell populations with MHC-II contributed more significantly to producing vaccine-specific IgG antibodies than CD43(-) B220(+) conventional B cell or CD4 T cell populations, as well as conferring protection against lethal infection. Bone marrow-derived dendritic cells from MHC-II KO mice showed a significant defect in producing interleukin-6 and tumor necrosis factor alpha cytokines. Thus, results indicate that MHC-II molecules play multiple roles in inducing protective immunity to influenza vaccination. Importance: Major histocompatibility complex class II (MHC-II) has been known to activate CD4 T helper immune cells. A deficiency in MHC-II was considered to be equivalent to the lack of CD4 T cells in developing host immune responses to pathogens. However, the roles of MHC-II in inducing protective immune responses to vaccination have not been well understood. In the present study, we demonstrate that MHC-II-deficient mice showed much more significant defects in inducing protective antibody responses to influenza vaccination than CD4 T cell-deficient mice. Further analysis showed that CD43 marker-positive immune cells with MHC-II, as well as an innate immunity-simulating adjuvant, could rescue some defects in inducing protective immune responses in MHC-II-deficient mice. These results have important implications for our understanding of host immunity-inducing mechanisms to vaccination, as well as in developing effective vaccines and adjuvants.
Assuntos
Antígenos de Histocompatibilidade Classe II/imunologia , Vírus da Influenza A/imunologia , Vacinas contra Influenza/imunologia , Leucócitos Mononucleares/imunologia , Transferência Adotiva , Animais , Anticorpos Antivirais/sangue , Feminino , Antígenos de Histocompatibilidade Classe II/análise , Antígenos de Histocompatibilidade Classe II/genética , Imunoglobulina G/sangue , Imunoglobulina M/sangue , Vacinas contra Influenza/administração & dosagem , Leucócitos Mononucleares/química , Leucossialina/análise , Camundongos , Camundongos Endogâmicos BALB C , Camundongos Endogâmicos C57BL , Camundongos Knockout , Vacinas Atenuadas/administração & dosagem , Vacinas Atenuadas/imunologia , Vacinas de Produtos Inativados/administração & dosagem , Vacinas de Produtos Inativados/imunologia , Vacinas de Partículas Semelhantes a Vírus/administração & dosagem , Vacinas de Partículas Semelhantes a Vírus/imunologiaRESUMO
PURPOSE: Patients with acute myeloid leukemia (AML) often present with fatigue and severe pancytopenia. We report the case of a 68-year-old woman with no significant medical history who presented with 1 year of progressively worsening bilateral temporomandibular joint (TMJ) pain. She was otherwise asymptomatic. A computed tomography scan revealed degenerative joint disease in both TMJs. Bilateral TMJ replacement was performed. MATERIALS AND METHODS: The excised TMJ tissue underwent formalin fixation and decalcification, and routine hematoxylin and eosin-stained sections were generated. RESULTS: Immunohistochemical stains showed a population of monotonous cells in the marrow space expressing CD33, CD43, and myeloperoxidase, confirming the diagnosis of myeloid neoplasm. Subsequent bone marrow biopsy with flow cytometry confirmed AML with myelodysplasia-related changes. CONCLUSIONS: Adult patients with AML can rarely present with musculoskeletal complaints alone, which could delay the diagnosis. To our knowledge, this is the first report of AML with myelodysplasia-related changes presenting in a patient with TMJ degenerative joint disease that was otherwise asymptomatic.
Assuntos
Artrite/diagnóstico , Leucemia Mieloide Aguda/diagnóstico , Síndromes Mielodisplásicas/diagnóstico , Transtornos da Articulação Temporomandibular/diagnóstico , Idoso , Medula Óssea/patologia , Feminino , Citometria de Fluxo/métodos , Seguimentos , Humanos , Leucossialina/análise , Osteoartrite/diagnóstico , Peroxidase/análise , Lectina 3 Semelhante a Ig de Ligação ao Ácido Siálico/análise , Tomografia Computadorizada por Raios X/métodosRESUMO
Stress triggers complex response mechanisms designed to recognize and adapt to perturbations in homeostasis. The immune system is highly responsive to stress, although the complete mechanisms linking stress and immune mediators including T lymphocytes, are not fully understood. Stress exerts its effects on immune effectors through two primary pathways: the sympathetic-adrenal-medullary pathway, and the hypothalamic-pituitary-adrenal pathway which modulate adaptive immunity and lymphocyte migration. In this report we show that stress via release of stress hormones induces early T cell activation and greatly impacts the cytoskeleton by modulating numerous actin-regulating proteins. In particular, proteomic profiling revealed significant decreases in numerous key actin-binding proteins including moesin. Although confocal microscopy showed that moesin and actin were uniformly distributed on the surface of resting T cells, a remarkable polarization and redistribution of moesin and actin was observed following treatment with stress hormones with moesin localizing at the distal pole complex. In addition, the alteration in moesin localization and eventual decrease in expression were accompanied by a loss of CD43; a receptor involved in negatively regulating T cell activation. In conclusion, we have defined a novel molecular mechanism whereby stress hormones negatively impact T cell activation and migration through regulation of key cytoskeletal and plasma membrane factors.
Assuntos
Citoesqueleto/ultraestrutura , Restrição Física/efeitos adversos , Estresse Fisiológico/imunologia , Estresse Psicológico/imunologia , Linfócitos T/imunologia , Actinas/biossíntese , Actinas/genética , Animais , Antígenos CD/biossíntese , Antígenos CD/genética , Antígenos de Diferenciação de Linfócitos T/biossíntese , Antígenos de Diferenciação de Linfócitos T/genética , Catecolaminas/fisiologia , Divisão Celular/efeitos dos fármacos , Movimento Celular/efeitos dos fármacos , Células Cultivadas/imunologia , Células Cultivadas/metabolismo , Células Cultivadas/ultraestrutura , Citoesqueleto/metabolismo , Feminino , Glucocorticoides/fisiologia , Ionomicina/farmacologia , Lectinas Tipo C/biossíntese , Lectinas Tipo C/genética , Leucossialina/análise , Lipopolissacarídeos/farmacologia , Ativação Linfocitária/efeitos dos fármacos , Camundongos , Camundongos Endogâmicos BALB C , Proteínas dos Microfilamentos/biossíntese , Proteínas dos Microfilamentos/genética , Proteômica , Estresse Fisiológico/fisiologia , Estresse Psicológico/fisiopatologia , Linfócitos T/efeitos dos fármacos , Linfócitos T/metabolismo , Linfócitos T/ultraestrutura , Acetato de Tetradecanoilforbol/farmacologiaRESUMO
Autoantibodies are central to the pathogenesis of several autoimmune diseases including systemic lupus erythematosus. Plasma cells secrete these autoantibodies, but the anatomical sites of these cells are not well defined. Here, we found that although dsDNA-specific plasma cells in NZB/W mice were present in spleen and bone marrow, a large number were in the kidneys and their number correlated with the serum dsDNA-IgG titer. We observed renal plasma cells only in mice with nephritis, where they located mainly to the tubulointerstitium of the cortex and outer medulla. These cells had the phenotypic characteristics of fully differentiated plasma cells and, similar to long-lived bone marrow plasma cells, they were not in cell cycle. In patients with lupus nephritis, plasma cells were often present in the medulla in those with the most severe disease, especially combined proliferative and membranous lupus nephritis. The identification of the kidney as a major site of autoreactive plasma cells has implications for our understanding of the pathogenesis of lupus nephritis and for strategies to deplete autoreactive plasma cells, a long-standing therapeutic aim.
Assuntos
Autoanticorpos/biossíntese , Rim/imunologia , Nefrite Lúpica/imunologia , Animais , Anticorpos Antinucleares/sangue , Membrana Basal/imunologia , Biópsia , Quimiocina CXCL12/análise , Feminino , Humanos , Antígeno Ki-67/análise , Rim/patologia , Leucossialina/análise , Lúpus Eritematoso Sistêmico/patologia , Nefrite Lúpica/etiologia , Nefrite Lúpica/patologia , Masculino , Camundongos , Camundongos Endogâmicos C57BL , Camundongos Endogâmicos NZB , Plasmócitos/imunologia , Plasmócitos/patologiaAssuntos
Neoplasias do Colo/diagnóstico , Neoplasias do Colo/patologia , Sarcoma Mieloide/diagnóstico , Sarcoma Mieloide/patologia , Colonoscopia , Histocitoquímica , Humanos , Imuno-Histoquímica , Leucossialina/análise , Masculino , Microscopia , Pessoa de Meia-Idade , Peroxidase/análise , Radiografia Abdominal , Tomografia Computadorizada por Raios XRESUMO
Previous studies have reported changes both in dental pulp and in periodontal ligament (PDL) following orthodontic tooth movement. However, pulpal changes following extensive root resorption after orthodontic tooth movement have not been studied in detail. The aim of this study was therefore to evaluate inflammatory changes, both in the dental pulp and in the compressed PDL, after experimentally induced extensive root resorption. Extensive root resorption was induced in rats by the activation and re-activation of orthodontic force, with a short intervening period of no force application. The distribution of immune cells, nerve fibres and blood vessels was studied immunohistochemically using antibodies against CD68-immunoreactive (IR) cells, major histocompatibility complex (MHC) class II Ia-expressing cells, CD43-IR cells, protein gene product 9.5 (PGP 9.5), and laminin. In the compressed PDL of experimental first molars, significantly increased density of CD68-IR cells and MHC class II Ia-expressing cells were found, whereas the density of CD43-IR cells were unchanged when compared with control second molars. In the compressed PDL, there was an increased density of blood vessels, but no sprouting of nerve fibres. In the dental pulp, however, no increased density of immune cells or sprouting of nerve fibres was recorded. In conclusion, inflammation after extensive root resorption was confined to the compressed PDL, whereas the dental pulp was unaffected.
Assuntos
Polpa Dentária/patologia , Ligamento Periodontal/patologia , Periodontite/etiologia , Pulpite/etiologia , Reabsorção da Raiz/etiologia , Técnicas de Movimentação Dentária/efeitos adversos , Animais , Antígenos CD/análise , Antígenos de Diferenciação Mielomonocítica/análise , Polpa Dentária/imunologia , Antígenos de Histocompatibilidade Classe II/análise , Laminina/análise , Leucócitos Mononucleares/patologia , Leucossialina/análise , Macrófagos/patologia , Masculino , Microvasos/patologia , Dente Molar/imunologia , Dente Molar/patologia , Fibras Nervosas/patologia , Ligamento Periodontal/imunologia , Periodontite/imunologia , Periodontite/patologia , Pressão , Pulpite/imunologia , Pulpite/patologia , Ratos , Ratos Sprague-Dawley , Reabsorção da Raiz/imunologia , Reabsorção da Raiz/patologia , Linfócitos T/patologia , Ubiquitina Tiolesterase/análiseAssuntos
Leucemia Mielomonocítica Crônica/patologia , Infiltração Leucêmica/diagnóstico , Pele/patologia , Toxoide Tetânico/efeitos adversos , Antígenos CD/análise , Antígenos de Diferenciação Mielomonocítica/análise , Protocolos de Quimioterapia Combinada Antineoplásica/uso terapêutico , Medula Óssea/patologia , Citarabina/administração & dosagem , Etoposídeo/administração & dosagem , Evolução Fatal , Humanos , Idarubicina/administração & dosagem , Imunização Secundária , Leucemia Mielomonocítica Crônica/diagnóstico , Leucemia Mielomonocítica Crônica/tratamento farmacológico , Leucemia Mielomonocítica Crônica/radioterapia , Infiltração Leucêmica/tratamento farmacológico , Infiltração Leucêmica/etiologia , Infiltração Leucêmica/patologia , Infiltração Leucêmica/radioterapia , Leucossialina/análise , Masculino , Pessoa de Meia-Idade , Radioterapia Adjuvante , Choque Séptico/etiologia , Toxoide Tetânico/administração & dosagemRESUMO
This study aimed to identify the frequency and distribution of developing B cell populations in the kidney of the rainbow trout, using four molecular B cell markers that are highly conserved between species, including two transcription factors, Pax5 and EBF1, recombination-activating gene RAG1, and the immunoglobulin heavy chain mu. Three distinct B cell stages were defined: early developing B cells (CLP, pro-B, and early pre-B cells), late developing B cell (late pre-B, immature B, and mature B cells), and IgM-secreting cells. Developmental stage-specific, combinatorial expression of Pax5, EBF1, RAG1 and immunoglobulin mu was determined in trout anterior kidney cells by flow cytometry. Trout staining patterns were compared to a well-defined primary immune tissue, mouse bone marrow, and using mouse surface markers B220 and CD43. A remarkable level of similarity was uncovered between the primary immune tissues of both species. Subsequent analysis of the entire trout kidney, divided into five contiguous segments K1-K5, revealed a complex pattern of early developing, late developing, and IgM-secreting B cells. Patterns in anterior kidney segment K1 were most similar to those of mouse bone marrow, while the most posterior part of the kidney, K5, had many IgM-secreting cells, but lacked early developing B cells. A potential second B lymphopoiesis site was uncovered in segment K4 of the kidney. The B cell patterns, or "B cell signatures" described here provide information on the relative abundance of distinct developing B cell populations in the trout kidney, and can be used in future studies on B cell development in other vertebrate species.
Assuntos
Linfócitos B/imunologia , Medula Óssea/imunologia , Rim/imunologia , Linfopoese , Truta/imunologia , Animais , Linfócitos B/metabolismo , Biomarcadores/análise , Western Blotting , Diferenciação Celular , Citometria de Fluxo , Expressão Gênica , Proteínas de Homeodomínio/análise , Cadeias Pesadas de Imunoglobulinas/análise , Cadeias mu de Imunoglobulina/análise , Antígenos Comuns de Leucócito/análise , Leucossialina/análise , Camundongos , Fator de Transcrição PAX5/análise , Células Precursoras de Linfócitos B , Transativadores/análise , Fatores de Transcrição/genética , Truta/genéticaRESUMO
Granulocytic sarcoma is an uncommon tumor composed of myeloid blasts and/or immature myeloid cells in an extramedullary site which is usually associated with acute or chronic myeloid leukemia. The tumor may also be the initial manifestation of leukemia. The histomorphological diagnosis of granulocytic sarcoma can be challenging to pathologists, especially in the absence of a known hematological disorder. In this case, differentiation of granulocytic sarcoma from malignant lymphomas and other small round cell tumors is very critical. Seven cases of granulocytic sarcoma are reported in this paper. One patient had granulocytic sarcomas at two different sites. Hematoxylin-eosin-stained sections were reexamined. Blastic, poorly differentiated, and well differentiated histopathological variants were found in two, five and one cases, respectively. Immunohistochemical studies were performed on formalin-fixed tissue from all cases using a avidin-biotin-peroxidase complex technique. The panel included antibodies against LCA, CD43, CD34, c-kit, myeloperoxidase, CD68 KP1, CD15, and CD99. All cases stained positively with LCA, CD43, CD34, myeloperoxidase, and CD68. Five cases were positive for c-kit, three cases were positive for CD15, and two cases were positive for CD99. An immunohistochemical panel including at least myeloperoxidase, CD68 and CD34 can be used for detection of myeloid differentiation. It is also important that granulocytic sarcoma be considered in the differential diagnosis of CD99-positive round cell tumors.
Assuntos
Biomarcadores Tumorais/análise , Sarcoma Mieloide/diagnóstico , Antígeno 12E7 , Adolescente , Adulto , Idoso , Antígenos CD/análise , Antígenos CD34/análise , Antígenos de Diferenciação Mielomonocítica/análise , Moléculas de Adesão Celular/análise , Diagnóstico Diferencial , Feminino , Fucosiltransferases/análise , Humanos , Imuno-Histoquímica/métodos , Leucossialina/análise , Antígenos CD15/análise , Masculino , Pessoa de Meia-Idade , Peroxidase/análise , Proteínas Proto-Oncogênicas c-kit/análise , Sarcoma Mieloide/classificação , Sarcoma Mieloide/metabolismo , Sarcoma Mieloide/patologiaRESUMO
Chronic lymphocytic leukemia (CLL) is a common hematologic malignancy associated with an increased risk of non-melanoma skin cancer. Basal cell carcinomas and squamous cell carcinomas in these patients may have an associated dense peritumoral leukemic infiltrate. This infiltrate can lead to the diagnosis of CLL and may also obscure tumor margins and pose a challenge in the assessment of perineural tumor spread. Immunohistochemical stains are useful in distinguishing leukemic B-cell infiltrates from tumor-reactive T-cell infiltrates. Leukemic cells of CLL are CD20+/CD23+/CD5+/CD43+/CD3-, whereas benign reactive infiltrates are composed of CD20-/CD23-/CD5+/CD43+/CD3+ T-cells. Given the paucity of symptoms in early stages of CLL, a dense lymphoid infiltrate surrounding a cutaneous neoplasm may serve as the first indication of CLL. We report a series of three cases of SCC with a coexisting infiltrate of CLL, including one with perineural involvement, one involving metastatic SCC, and one in which this histologic finding spurred the initial diagnosis of CLL.