Phytotoxic, cytogenotoxic, and insecticidal activities of compounds from extracts of freshwater Lyngbya sp.

The development of agroecology has promoted the discovery of new bioactive compounds that might act as biocides to control infections and microbial contamination. Algae belonging to Lyngbya genus produce several allelochemicals, which are compounds with crop protection potential. The present study aimed to examine primary and secondary compounds derived from Lyngbya sp. extracts (aqueous and hydroethanolic) on phytotoxic, cytogenotoxic, and insecticidal activities. Determination of compounds indicated the presence predominantly of proteins and flavonoids. The extracts presented physicochemical characteristics that produced (1) 89% germination inhibition using hydroethanolic extract and (2) diminished development of seedlings of L. sativa by hydroethanolic extract as evidenced by reduced radicles length in 83.54%. Aqueous and hydroethanolic Lyngbya sp. extracts significantly interfered with meristematic cells of A. cepa, as evidenced by chromosomal alterations and aberrant mitotic phases in cells. Extracts also exhibited pro-oxidative activity and a potent insecticidal potential on S. zeamais, indicating that the hydroethanolic extract produced 100% insect mortality at 75 mg/ml after 48 hr while the aqueous extract initiated 90% mortality at the same concentration after 82 hr. Therefore, data demonstrate that Lyngbya genus provides basic information for new environmental and ecotoxicological studies to seek a possible source of proteins and flavonoids to be used in agroecological management.

Kalkitoxin Reduces Osteoclast Formation and Resorption and Protects against Inflammatory Bone Loss.

Osteoclasts, bone-specified multinucleated cells produced by monocyte/macrophage, are involved in numerous bone destructive diseases such as arthritis, osteoporosis, and inflammation-induced bone loss. The osteoclast differentiation mechanism suggests a possible strategy to treat bone diseases. In this regard, we recently examined the in vivo impact of kalkitoxin (KT), a marine product obtained from the marine cyanobacterium Moorena producens (previously Lyngbya majuscula), on the macrophage colony-stimulating factor (M-CSF) and on the receptor activator of nuclear factor κB ligand (RANKL)-stimulated in vitro osteoclastogenesis and inflammation-mediated bone loss. We have now examined the molecular mechanism of KT in greater detail. KT decreased RANKL-induced bone marrow-derived macrophages (BMMs) tartrate-resistant acid phosphatase (TRAP)-multinucleated cells at a late stage. Likewise, KT suppressed RANKL-induced pit area and actin ring formation in BMM cells. Additionally, KT inhibited several RANKL-induced genes such as cathepsin K, matrix metalloproteinase (MMP-9), TRAP, and dendritic cell-specific transmembrane protein (DC-STAMP). In line with these results, RANKL stimulated both genes and protein expression of c-Fos and nuclear factor of activated T cells (NFATc1), and this was also suppressed by KT. Moreover, KT markedly decreased RANKL-induced p-ERK1/2 and p-JNK pathways at different time points. As a result, KT prevented inflammatory bone loss in mice, such as bone mineral density (BMD) and osteoclast differentiation markers. These experiments demonstrated that KT markedly inhibited osteoclast formation and inflammatory bone loss through NFATc1 and mitogen-activated protein kinase (MAPK) signaling pathways. Therefore, KT may have potential as a treatment for destructive bone diseases.