Assuntos
Herpesvirus Humano 4 , Mononucleose Infecciosa/complicações , Serosite/virologia , Membrana Serosa/virologia , Doença Aguda , Adolescente , Adulto , Idoso , Idoso de 80 Anos ou mais , Criança , Pré-Escolar , Feminino , Humanos , Lactente , Mononucleose Infecciosa/diagnóstico , Masculino , Pessoa de Meia-Idade , Adulto JovemRESUMO
Peritonitis of viral etiology is rarely reported in the literature; a prior report described a patient undergoing continuous ambulatory peritoneal dialysis who had the disease. We report a case of primary herpetic peritonitis (the agent of which was typed by polymerase chain reaction as herpes simplex virus biotype 1), which caused intestinal perforation, and we review the current literature and provide possible pathophysiologic mechanisms.
Assuntos
Herpes Simples/complicações , Herpesvirus Humano 1 , Perfuração Intestinal/etiologia , Peritonite/virologia , Feminino , Herpesvirus Humano 1/isolamento & purificação , Humanos , Técnicas Imunoenzimáticas , Corpos de Inclusão Viral , Pessoa de Meia-Idade , Peritonite/complicações , Membrana Serosa/virologiaRESUMO
The prevalence and cellular distribution of human herpesvirus 7 (HHV-7) in archival labial salivary glands was analysed for virus-specific DNA sequences by polymerase chain reaction (PCR) and in situ hybridization signals. In addition, the cellular expression of HHV-7-encoded protein was detected by immunohistochemical staining with a virus-specific monoclonal antibody. Eleven of 20 samples were positive for the HHV-7 DNA sequence by PCR. Eighteen of 20 tissues analysed by in situ hybridization showed signals in ductal, serous and mucous cells. Some nuclei of these cells and also the myoepithelial population were positive. In immunolocalization studies, all 20 salivary glands consistently showed HHV-7-expressed protein in the cytoplasm of ductal cuboidal and columnar cells. The protein was also found in the cytoplasm of mucous and serous acinar cells that were immunopositive for HHV-7. The observations are consistent with the suggestion that the labial salivary gland is a site for virus replication, potential persistence and a source of infective HHV-7 in saliva.
Assuntos
Herpesvirus Humano 7/isolamento & purificação , Glândulas Salivares/virologia , Adolescente , Adulto , Anticorpos Monoclonais , Southern Blotting , Núcleo Celular/ultraestrutura , Núcleo Celular/virologia , Criança , Citoplasma/ultraestrutura , Citoplasma/virologia , DNA Viral/análise , Células Epiteliais/citologia , Células Epiteliais/virologia , Feminino , Regulação Viral da Expressão Gênica , Infecções por Herpesviridae , Herpesvirus Humano 7/genética , Humanos , Imuno-Histoquímica , Hibridização In Situ , Masculino , Mucosa/citologia , Mucosa/virologia , Reação em Cadeia da Polimerase , Saliva/virologia , Ductos Salivares/citologia , Ductos Salivares/virologia , Glândulas Salivares/citologia , Análise de Sequência de DNA , Membrana Serosa/citologia , Membrana Serosa/virologia , Proteínas Virais/análise , Proteínas Virais/genética , Latência Viral , Replicação ViralRESUMO
Reports connect Kaposi's sarcoma-associated herpesvirus (KSHV) with various types of Kaposi's sarcoma (KS) and body-cavity-based lymphomas (primary effusion lymphomas). KSHV DNA sequences have also been detected in a few non-KS malignant vascular tumors. To determine whether KSHV is associated with malignant vascular tumors involving the body cavities, 11 primary vascular tumors of the serous membranes (4 angiosarcomas, 7 hemangioendotheliomas) were tested for KSHV sequences by polymerase chain reaction and Southern blot hybridization. The tumors were from patients who were not known to be immunocompromised. KSHV sequences were detected in the penile KS control but were absent in all 11 specimens studied. Our results were consistent with previous reports that KSHV sequences were rarely detected in non-KS vascular lesions. The results also suggested that although KSHV sequences might be present in primary effusion tumors such as primary effusion lymphoma, they might not be found in other tumors related to body cavities or to serous membranes.