RESUMO
We report the findings of the immunophenotypic profile of three cases of nasal T/NK cell lymphoma in leukemic phase. Flow cytometry analysis was carried out using cell suspensions of tumor nasal biopsies and peripheral blood. Tumor samples were composed by a mixture of a predominant subset of medium-size true NK cytCD3epsilon-, sCD3epsilon-, CD56+ cells mixed with a minor subset of medium-size T/NK sCD3epsilon+, CD56+ cells. Both subsets were also detected in peripheral blood. In addition, an infiltration of small-size sCD3epsilon+, CD56- normal T lymphocytes was also present.
Assuntos
Citometria de Fluxo , Células Matadoras Naturais/patologia , Leucemia Linfocítica Crônica de Células B/patologia , Linfócitos do Interstício Tumoral/patologia , Neoplasias Nasofaríngeas/patologia , Neoplasias Nasais/patologia , Subpopulações de Linfócitos T/patologia , Adulto , Idoso , Antígenos CD/análise , Biomarcadores Tumorais/análise , Biópsia , Infecções por Vírus Epstein-Barr/sangue , Infecções por Vírus Epstein-Barr/patologia , Feminino , Humanos , Imunofenotipagem , Células Matadoras Naturais/química , Células Matadoras Naturais/virologia , Leucemia Linfocítica Crônica de Células B/sangue , Linfócitos do Interstício Tumoral/química , Masculino , Neoplasias do Seio Maxilar/sangue , Neoplasias do Seio Maxilar/patologia , Neoplasias Nasofaríngeas/sangue , Neoplasias Nasais/sangue , Receptores KIR/análise , Subpopulações de Linfócitos T/química , Proteínas da Matriz Viral/análiseRESUMO
We tested for squamous cell carcinoma-related antigen (SCC), carcinoembryonic antigen (CEA), ferritin, immunosuppressive acid protein (IAP) and sialic acid in the serum from 247 patients with head and neck squamous cell carcinoma prior to therapy. Significant correlations were found between IAP and tumor size, lymph node metastasis, and clinical stage (P<0.0001, P<0.001, and P<0.0001). Also, sialic acid and SCC were also correlated with tumor size, lymph node metastasis, and clinical stage. Moreover IAP, sialic acid and SCC were strongly associated with survival rate (P<0.0001, P = 0.0230 and P = 0.0159). A multivariate Cox proportional hazard model demonstrated that being positive for IAP was an independent predictor for patients with H&NSCC (P = 0.0115). The results indicate that IAP, sialic acid and SCC are useful as prognostic factors.
Assuntos
Biomarcadores Tumorais/sangue , Carcinoma de Células Escamosas/sangue , Neoplasias de Cabeça e Pescoço/sangue , Serpinas , Antígenos de Neoplasias/sangue , Antígeno Carcinoembrionário/sangue , Carcinoma de Células Escamosas/diagnóstico , Carcinoma de Células Escamosas/mortalidade , Intervalo Livre de Doença , Ferritinas/sangue , Neoplasias de Cabeça e Pescoço/diagnóstico , Neoplasias de Cabeça e Pescoço/mortalidade , Humanos , Neoplasias Hipofaríngeas/sangue , Neoplasias Laríngeas/sangue , Metástase Linfática , Neoplasias do Seio Maxilar/sangue , Análise Multivariada , Ácido N-Acetilneuramínico/sangue , Neoplasias Nasofaríngeas/sangue , Proteínas de Neoplasias/sangue , Neoplasias Orofaríngeas/sangue , Prognóstico , Modelos de Riscos Proporcionais , Fatores de TempoRESUMO
We report a case of sinonasal lymphoma with a natural killer (NK) phenotype. This 40-year-old man was admitted to our hospital because of left nasal obstruction. Physical examination and computed tomography of the skull revealed a tumor in the left nasal cavity and maxillary sinus. Histopathological examination revealed a diffuse proliferation of pleomorphic lymphoid cells. Imprint cytology showed that tumor cells contained some azurophilic granules, and expressed CD2, CD8, CD16, CD56 and HLA-DR antigens with little expression of other lymphoid or myeloid markers. Southern blot analysis revealed germline configuration for immunoglobulin heavy chain and T-cell receptor genes. These findings indicated that these cells were in fact NK cells. The patient's enlarged pancreas was also involved by lymphoma and the pattern of involvement simulated that seen in primary pancreatic lymphoma. Ulcerative colitis (UC) was also present, a rare finding in this disorder.