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1.
Planta Med ; 84(1): 42-48, 2018 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-28701020

RESUMO

The genetic barcode ITS2 (ITS: internal transcribed spacer) and pollen morphology were used for the identification of the pharmacologically valuable wild Araliaceae species Panax ginseng, Oplopanax elatus, Aralia elata, Aralia continentalis, Eleutherococcus senticosus, and Eleutherococcus sessiliflorus inhabiting the natural forests of Primorye, Russia. The ITS2 locus successfully identified all six species, which supports the use of ITS2 as a standard barcode for medicinal plants. However, the ITS2 locus was insufficient for intra-specific discrimination in these species, neither within Primorye nor from other world representatives within GenBank. Araliaceae pollen was confirmed to undergo size-reducing metamorphosis. The final morphotypes were species-specific for each of the six species but could not discriminate intra-species geographic localities within Primorye. The morphologies of the final pollen morphotypes from homologous species inhabiting other parts of the world are not yet known. Therefore, whether pollen is applicable for Araliaceae intra-species discrimination between Primorye and other world localities could not be established. Based on these findings, we propose that the ITS2 genetic barcode and the final pollen morphotypes are suitable for the identification of Araliaceae species. However, further studies will be needed to determine the suitability of genetic and pollen traits for Araliaceae geographic authentication.


Assuntos
Araliaceae/genética , Código de Barras de DNA Taxonômico/métodos , Pólen/ultraestrutura , Aralia/genética , Aralia/ultraestrutura , Araliaceae/ultraestrutura , DNA Espaçador Ribossômico/genética , Eleutherococcus/genética , Eleutherococcus/ultraestrutura , Oplopanax/genética , Oplopanax/ultraestrutura , Panax/genética , Panax/ultraestrutura , Filogenia , Especificidade da Espécie
3.
Genetika ; 46(5): 631-9, 2010 May.
Artigo em Russo | MEDLINE | ID: mdl-20583598

RESUMO

Using allozyme analysis, genetic and genotypic variation of rare relict species, a member of one of ancient angiosperm families, Araliaceae, the clone-forming plant Oplopanax elatus (Nakai) Nakai, was evaluated. Electrophoretic separation of the enzymes is described, and genetic interpretation of the enzymes variation patterns is presented. The values of genetic variation indices obtained were low (P = 25%; A = 1.45: Ho = 0.131; He = 0.113) and comparable with the data reported for the rare plant species and the representatives of the family Araliaceae. The main factors responsible for the polymorphism level observed might be the evolutionary history of the species and gene drift. The level of genotypic diversity (G/N = 0.76; D = 0.97) was substantially higher, compared to the values reported for species with vegetative reproduction (D = 0.62). These results suggest certain contribution of the propagation by seeds in the formation of the species of interest.


Assuntos
Evolução Molecular , Deriva Genética , Oplopanax/genética , Polimorfismo Genético , Oplopanax/enzimologia , Proteínas de Plantas/genética
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