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1.
Commun Biol ; 4(1): 776, 2021 06 23.
Artigo em Inglês | MEDLINE | ID: mdl-34163006

RESUMO

Agonist bias occurs when different ligands produce distinct signalling outputs when acting at the same receptor. However, its physiological relevance is not always clear. Using primary human cells and gene editing techniques, we demonstrate endogenous agonist bias with physiological consequences for the calcitonin receptor-like receptor, CLR. By switching the receptor-activity modifying protein (RAMP) associated with CLR we can "re-route" the physiological pathways activated by endogenous agonists calcitonin gene-related peptide (CGRP), adrenomedullin (AM) and adrenomedullin 2 (AM2). AM2 promotes calcium-mediated nitric oxide signalling whereas CGRP and AM show pro-proliferative effects in cardiovascular cells, thus providing a rationale for the expression of the three peptides. CLR-based agonist bias occurs naturally in human cells and has a fundamental purpose for its existence. We anticipate this will be a starting point for more studies into RAMP function in native environments and their importance in endogenous GPCR signalling.


Assuntos
Adrenomedulina/fisiologia , Peptídeo Relacionado com Gene de Calcitonina/fisiologia , Hormônios Peptídicos/fisiologia , Receptores Acoplados a Proteínas G/agonistas , Proteína Semelhante a Receptor de Calcitonina/fisiologia , Células Cultivadas , AMP Cíclico/metabolismo , Células Endoteliais/fisiologia , MAP Quinases Reguladas por Sinal Extracelular/metabolismo , Humanos , Receptores de Adrenomedulina/agonistas , Receptores de Adrenomedulina/análise , Receptores de Peptídeo Relacionado com o Gene de Calcitonina/fisiologia
2.
Front Endocrinol (Lausanne) ; 12: 792912, 2021.
Artigo em Inglês | MEDLINE | ID: mdl-35095763

RESUMO

The first intracellular loop (ICL1) of G protein-coupled receptors (GPCRs) has received little attention, although there is evidence that, with the 8th helix (H8), it is involved in early conformational changes following receptor activation as well as contacting the G protein ß subunit. In class B1 GPCRs, the distal part of ICL1 contains a conserved R12.48KLRCxR2.46b motif that extends into the base of the second transmembrane helix; this is weakly conserved as a [R/H]12.48KL[R/H] motif in class A GPCRs. In the current study, the role of ICL1 and H8 in signaling through cAMP, iCa2+ and ERK1/2 has been examined in two class B1 GPCRs, using mutagenesis and molecular dynamics. Mutations throughout ICL1 can either enhance or disrupt cAMP production by CGRP at the CGRP receptor. Alanine mutagenesis identified subtle differences with regard elevation of iCa2+, with the distal end of the loop being particularly sensitive. ERK1/2 activation displayed little sensitivity to ICL1 mutation. A broadly similar pattern was observed with the glucagon receptor, although there were differences in significance of individual residues. Extending the study revealed that at the CRF1 receptor, an insertion in ICL1 switched signaling bias between iCa2+ and cAMP. Molecular dynamics suggested that changes in ICL1 altered the conformation of ICL2 and the H8/TM7 junction (ICL4). For H8, alanine mutagenesis showed the importance of E3908.49b for all three signal transduction pathways, for the CGRP receptor, but mutations of other residues largely just altered ERK1/2 activation. Thus, ICL1 may modulate GPCR bias via interactions with ICL2, ICL4 and the Gß subunit.


Assuntos
Motivos de Aminoácidos/fisiologia , Receptores de Peptídeo Relacionado com o Gene de Calcitonina/ultraestrutura , Receptores de Hormônio Liberador da Corticotropina/ultraestrutura , Receptores de Glucagon/ultraestrutura , Proteína Semelhante a Receptor de Calcitonina/metabolismo , Proteína Semelhante a Receptor de Calcitonina/fisiologia , Proteína Semelhante a Receptor de Calcitonina/ultraestrutura , Sinalização do Cálcio , AMP Cíclico/metabolismo , Células HEK293 , Humanos , Sistema de Sinalização das MAP Quinases , Simulação de Dinâmica Molecular , Domínios Proteicos , Estrutura Terciária de Proteína , Proteína 1 Modificadora da Atividade de Receptores/metabolismo , Proteína 1 Modificadora da Atividade de Receptores/fisiologia , Proteína 1 Modificadora da Atividade de Receptores/ultraestrutura , Receptores de Peptídeo Relacionado com o Gene de Calcitonina/metabolismo , Receptores de Peptídeo Relacionado com o Gene de Calcitonina/fisiologia , Receptores de Hormônio Liberador da Corticotropina/metabolismo , Receptores de Hormônio Liberador da Corticotropina/fisiologia , Receptores Acoplados a Proteínas G , Receptores de Glucagon/metabolismo , Receptores de Glucagon/fisiologia
3.
JCI Insight ; 5(23)2020 12 03.
Artigo em Inglês | MEDLINE | ID: mdl-33268595

RESUMO

Atherosclerosis develops preferentially in areas of the arterial system, in which blood flow is disturbed. Exposure of endothelial cells to disturbed flow has been shown to induce inflammatory signaling, including NF-κB activation, which leads to the expression of leukocyte adhesion molecules and chemokines. Here, we show that disturbed flow promotes the release of adrenomedullin from endothelial cells, which in turn activates its Gs-coupled receptor calcitonin receptor-like receptor (CALCRL). This induces antiinflammatory signaling through cAMP and PKA, and it results in reduced endothelial inflammation in vitro and in vivo. Suppression of endothelial expression of Gαs, the α subunit of the G-protein Gs; CALCRL; or adrenomedullin leads to increased disturbed flow-induced inflammatory signaling in vitro and in vivo. Furthermore, mice with induced endothelial-specific deficiency of Gαs, CALCRL, or adrenomedullin show increased atherosclerotic lesions. Our data identify an antiinflammatory signaling pathway in endothelial cells stimulated by disturbed flow and suggest activation of the endothelial adrenomedullin/CALCRL/Gs system as a promising approach to inhibit progression of atherosclerosis.


Assuntos
Adrenomedulina/metabolismo , Circulação Sanguínea/fisiologia , Proteína Semelhante a Receptor de Calcitonina/metabolismo , Animais , Aterosclerose/patologia , Proteína Semelhante a Receptor de Calcitonina/fisiologia , Bovinos , Moléculas de Adesão Celular/metabolismo , Células Endoteliais/metabolismo , Endotélio Vascular/metabolismo , Células Endoteliais da Veia Umbilical Humana/citologia , Humanos , Inflamação/metabolismo , Camundongos , NF-kappa B/metabolismo , Cultura Primária de Células , Transdução de Sinais , Molécula 1 de Adesão de Célula Vascular/metabolismo
4.
PLoS One ; 10(4): e0123697, 2015.
Artigo em Inglês | MEDLINE | ID: mdl-25860809

RESUMO

Cerebral blood flow autoregulation (CA) shifts to higher blood pressures in chronic hypertensive patients, which increases their risk for brain damage. Although cerebral vascular smooth muscle cells express the potent vasodilatatory peptides calcitonin gene-related peptide (CGRP) and adrenomedullin (AM) and their receptors (calcitonin receptor-like receptor (Calclr), receptor-modifying proteins (RAMP) 1 and 2), their contribution to CA during chronic hypertension is poorly understood. Here we report that chronic (10 weeks) hypertensive (one-kidney-one-clip-method) mice overexpressing the Calclr in smooth muscle cells (CLR-tg), which increases the natural sensitivity of the brain vasculature to CGRP and AM show significantly better blood pressure drop-induced cerebrovascular reactivity than wt controls. Compared to sham mice, this was paralleled by increased cerebral CGRP-binding sites (receptor autoradiography), significantly in CLR-tg but not wt mice. AM-binding sites remained unchanged. Whereas hypertension did not alter RAMP-1 expression (droplet digital (dd) PCR) in either mouse line, RAMP-2 expression dropped significantly in both mouse lines by about 65%. Moreover, in wt only Calclr expression was reduced by about 70% parallel to an increase of smooth muscle actin (Acta2) expression. Thus, chronic hypertension induces a stoichiometric shift between CGRP and AM receptors in favor of the CGRP receptor. However, the parallel reduction of Calclr expression observed in wt mice but not CLR-tg mice appears to be a key mechanism in chronic hypertension impairing cerebrovascular reactivity.


Assuntos
Circulação Cerebrovascular/fisiologia , Hipertensão/fisiopatologia , Receptores de Peptídeo Relacionado com o Gene de Calcitonina/fisiologia , Adrenomedulina/fisiologia , Animais , Sítios de Ligação , Encéfalo/fisiopatologia , Proteína Semelhante a Receptor de Calcitonina/genética , Proteína Semelhante a Receptor de Calcitonina/fisiologia , Circulação Cerebrovascular/genética , Feminino , Hipertensão/genética , Camundongos , Camundongos Endogâmicos DBA , Camundongos Transgênicos , Dados de Sequência Molecular , Ratos , Proteína 1 Modificadora da Atividade de Receptores/genética , Proteína 1 Modificadora da Atividade de Receptores/fisiologia , Proteína 2 Modificadora da Atividade de Receptores/genética , Proteína 2 Modificadora da Atividade de Receptores/fisiologia , Receptores de Peptídeo Relacionado com o Gene de Calcitonina/genética , Proteínas Recombinantes/genética , Proteínas Recombinantes/metabolismo
5.
Bone ; 52(1): 83-92, 2013 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-23017661

RESUMO

Enzymatically released cells from neonatal mouse calvarial bones are frequently used as primary mouse osteoblasts for in vitro studies. We, here, report that although these cells lack mRNA expression of the osteoclastic genes Calcr, Acp5 and Mmp-9 at early time points after their isolation, these transcripts are gradually upregulated when the calvarial osteoblast cultures are differentiated to more mature osteoblasts in long term cultures. Similarly, Calcr, Acp5, Mmp-9, as well as Rank and Nfatc1 mRNA expressions are robustly increased when the osteoblast cultures were induced to differentiate by treatment with bone morphogenetic protein (BMP-2). The increased Calcr mRNA resulted in functionally active calcitonin receptors. Enhanced osteoblastic differentiation was associated with increased Rankl mRNA expression and decreased Opg and Cfs1 mRNA expression. Treatment of the osteoblastic cells with BMP-2 or RANKL, either on plastic dishes or bone slices, resulted in the formation of multinucleated tartrate-resistant acid phosphatase positive cells, which were able to excavate resorption pits and release CTX from the bones. In contrast, increased osteoblastic differentiation induced by BMP-2 in the mouse calvarial osteoblastic cell line MC3T3-E1 was not associated with increased mRNA expression of Calcr, Acp5, Rank, c-Fms or Oscar. Interestingly, Ctsk mRNA was increased during osteoblastic differentiation in both mouse calvarial osteoblast cultures and in MC3T3-E1 cultures. Also osteoblasts isolated from mouse long bones by outgrowth from explant cultures were contaminated with osteoclast progenitors able to differentiate into bone resorbing osteoclasts. These data indicate that mouse calvarial osteoblast cultures contain osteoclast progenitor cells, which will be differentiated along the osteoclastic lineage during osteoblastic differentiation. Moreover, the data show that BMP-2 not only stimulates osteoblastic differentiation but can also induce osteoclastogenesis through increased RANKL.


Assuntos
Proteína Morfogenética Óssea 2/fisiologia , Proteína Semelhante a Receptor de Calcitonina/fisiologia , Osteoblastos/citologia , Osteoclastos/citologia , Células 3T3 , Animais , Calcitonina/metabolismo , Camundongos , Reação em Cadeia da Polimerase em Tempo Real
6.
Yakugaku Zasshi ; 132(11): 1211-5, 2012.
Artigo em Japonês | MEDLINE | ID: mdl-23123709

RESUMO

Skin inflammation is one of several allergic symptoms that are regulated by several mediator molecules. One of these molecules, calcitonin gene-related peptide (CGRP) affects several immune cells including T cells, B cells, dendiritic cells and mast cells. CGRP binds to CGRP receptors composed of receptor activity-modifying protein 1 (RAMP1) and calcitonin receptor-like receptor (CLR) to modulate various functions such as pain transmission and vasodilation. Studies showing that CGRP physiologically regulates skin inflammation using a CGRP antagonist, capsaicin-induced depletion model, RAMP1-deficient mice and mouse contact hypersensitivity (CHS) model have been reported. Interestingly, while CGRP has inhibitory effects on Th1-mediated CHS, it was demonstrated that CGRP enhances Th2-mediated CHS response. Moreover, these skin inflammations were affected by elevated CGRP concentrations through an abnormal condition of the nervous system induced by exposure to psychological stress or neonatal chemical stimulation. In this review, we present the importance of CGRP in the regulation of skin inflammation under the several nervous conditions and provide a new insight into understanding various types of skin inflammation and skin diseases.


Assuntos
Peptídeo Relacionado com Gene de Calcitonina/fisiologia , Imunomodulação/genética , Dermatopatias/imunologia , Animais , Peptídeo Relacionado com Gene de Calcitonina/antagonistas & inibidores , Peptídeo Relacionado com Gene de Calcitonina/imunologia , Peptídeo Relacionado com Gene de Calcitonina/metabolismo , Proteína Semelhante a Receptor de Calcitonina/metabolismo , Proteína Semelhante a Receptor de Calcitonina/fisiologia , Células Dendríticas/imunologia , Dermatite de Contato/imunologia , Humanos , Inflamação/imunologia , Proteína 1 Modificadora da Atividade de Receptores/metabolismo , Proteína 1 Modificadora da Atividade de Receptores/fisiologia , Receptores de Peptídeo Relacionado com o Gene de Calcitonina/imunologia , Estresse Psicológico/imunologia , Células Th1/imunologia , Células Th2/imunologia
7.
Br J Pharmacol ; 167(8): 1679-90, 2012 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-22881710

RESUMO

BACKGROUND AND PURPOSE: Calcitonin gene-related peptide (CGRP) is a potent vasodilator, implicated in the pathogenesis of migraine. CGRP activates a receptor complex comprising, calcitonin receptor-like receptor (CLR) and receptor activity-modifying protein 1 (RAMP1). In vitro studies indicate recycling of CLR●RAMP1 is regulated by degradation of CGRP in early endosomes by endothelin-converting enzyme-1 (ECE-1). However, it is not known if ECE-1 regulates the resensitization of CGRP-induced responses in functional arterial tissue. EXPERIMENTAL APPROACH: CLR, ECE-1a-d and RAMP1 expression in rat mesenteric artery smooth muscle cells (RMA-SMCs) and mesenteric arteries was analysed by RT-PCR and by immunofluorescence and confocal microscopy. CGRP-induced signalling in cells was examined by measuring cAMP production and ERK activation. CGRP-induced relaxation of arteries was measured by isometric wire myography. ECE-1 was inhibited using the specific inhibitor, SM-19712. KEY RESULTS: RMA-SMCs and arteries contained mRNA for CLR, ECE-1a-d and RAMP1. ECE-1 was present in early endosomes of RMA-SMCs and in the smooth muscle layer of arteries. CGRP induced endothelium-independent relaxation of arteries. ECE-1 inhibition had no effect on initial CGRP-induced responses but reduced cAMP generation in RMA-SMCs and vasodilation in mesenteric arteries responses to subsequent CGRP challenges. CONCLUSIONS AND IMPLICATIONS: ECE-1 regulated the resensitization of responses to CGRP in RMA-SMCs and mesenteric arteries. CGRP-induced relaxation did not involve endothelium-derived pathways. This is the first report of ECE-1 regulating CGRP responses in SMCs and arteries. ECE-1 inhibitors may attenuate an important vasodilatory pathway, implicated in primary headaches and may represent a new therapeutic approach for the treatment of migraine.


Assuntos
Ácido Aspártico Endopeptidases/fisiologia , Peptídeo Relacionado com Gene de Calcitonina/fisiologia , Artérias Mesentéricas/fisiologia , Metaloendopeptidases/fisiologia , Miócitos de Músculo Liso/fisiologia , Animais , Pressão Sanguínea/fisiologia , Proteína Semelhante a Receptor de Calcitonina/fisiologia , Células Cultivadas , Endossomos/fisiologia , Enzimas Conversoras de Endotelina , Masculino , Artérias Mesentéricas/citologia , Proteólise , RNA Mensageiro/metabolismo , Ratos , Ratos Wistar , Proteína 1 Modificadora da Atividade de Receptores/fisiologia , Vasodilatação/fisiologia
9.
Endocrinology ; 153(4): 1850-60, 2012 Apr.
Artigo em Inglês | MEDLINE | ID: mdl-22315449

RESUMO

Calcitonin gene-related peptide (CGRP) is a neuropeptide with multiple neuroendocrine roles, including vasodilation, migraine, and pain. The receptor for CGRP is a G protein-coupled receptor (GPCR) that requires three proteins for function. CGRP binds to a heterodimer composed of the GPCR calcitonin-like receptor (CLR) and receptor activity-modifying protein (RAMP1), a single transmembrane protein required for pharmacological specificity and trafficking of the CLR/RAMP1 complex to the cell surface. In addition, the CLR/RAMP1 complex requires a third protein named CGRP-receptor component protein (RCP) for signaling. Previous studies have demonstrated that depletion of RCP from cells inhibits CLR signaling, and in vivo studies have demonstrated that expression of RCP correlates with CLR signaling and CGRP efficacy. It is not known whether RCP interacts directly with CLR to exert its effect. The current studies identified a direct interaction between RCP and an intracellular domain of CLR using yeast two-hybrid analysis and coimmunoprecipitation. When this interacting domain of CLR was expressed as a soluble fusion protein, it coimmunoprecipitated with RCP and inhibited signaling from endogenous CLR. Expression of this dominant-negative domain of CLR did not significantly inhibit trafficking of CLR to the cell surface, and thus RCP may not have a chaperone function for CLR. Instead, RCP may regulate CLR signaling in the cell membrane, and direct interaction between RCP and CLR is required for CLR activation. To date, RCP has been found to interact only with CLR and represents a novel neuroendocrine regulatory step in GPCR signaling.


Assuntos
Proteína Semelhante a Receptor de Calcitonina/fisiologia , Receptores de Peptídeo Relacionado com o Gene de Calcitonina/fisiologia , Transdução de Sinais/fisiologia , Animais , Células Cultivadas , Proteínas de Fluorescência Verde , Camundongos , Modelos Animais , Células NIH 3T3 , Estrutura Terciária de Proteína/fisiologia , Proteína 1 Modificadora da Atividade de Receptores/fisiologia , Proteínas Recombinantes de Fusão
10.
Biochim Biophys Acta ; 1813(10): 1906-16, 2011 Oct.
Artigo em Inglês | MEDLINE | ID: mdl-21703310

RESUMO

The first and third extracellular loops (ECL) of G protein-coupled receptors (GPCRs) have been implicated in ligand binding and receptor function. This study describes the results of an alanine/leucine scan of ECLs 1 and 3 and loop-associated transmembrane (TM) domains of the secretin-like GPCR calcitonin receptor-like receptor which associates with receptor activity modifying protein 1 to form the CGRP receptor. Leu195Ala, Val198Ala and Ala199Leu at the top of TM2 all reduced αCGRP-mediated cAMP production and internalization; Leu195Ala and Ala199Leu also reduced αCGRP binding. These residues form a hydrophobic cluster within an area defined as the "minor groove" of rhodopsin-like GPCRs. Within ECL1, Ala203Leu and Ala206Leu influenced the ability of αCGRP to stimulate adenylate cyclase. In TM3, His219Ala, Leu220Ala and Leu222Ala have influences on αCGRP binding and cAMP production; they are likely to indirectly influence the binding site for αCGRP as well as having an involvement in signal transduction. On the exofacial surfaces of TMs 6 and 7, a number of residues were identified that reduced cell surface receptor expression, most noticeably Leu351Ala and Glu357Ala in TM6. The residues may contribute to the RAMP1 binding interface. Ile360Ala impaired αCGRP-mediated cAMP production. Ile360 is predicted to be located close to ECL2 and may facilitate receptor activation. Identification of several crucial functional loci gives further insight into the activation mechanism of this complex receptor system and may aid rational drug design.


Assuntos
Peptídeo Relacionado com Gene de Calcitonina/química , Peptídeo Relacionado com Gene de Calcitonina/fisiologia , Proteína Semelhante a Receptor de Calcitonina/química , Proteína Semelhante a Receptor de Calcitonina/fisiologia , Domínios e Motivos de Interação entre Proteínas/fisiologia , Estrutura Secundária de Proteína/fisiologia , Sequência de Aminoácidos , Substituição de Aminoácidos , Animais , Células COS , Peptídeo Relacionado com Gene de Calcitonina/genética , Peptídeo Relacionado com Gene de Calcitonina/metabolismo , Proteína Semelhante a Receptor de Calcitonina/genética , Proteína Semelhante a Receptor de Calcitonina/metabolismo , Bovinos , Membrana Celular/metabolismo , Chlorocebus aethiops , AMP Cíclico/metabolismo , Humanos , Modelos Biológicos , Modelos Moleculares , Dados de Sequência Molecular , Mutagênese Sítio-Dirigida , Proteínas Mutantes/química , Proteínas Mutantes/metabolismo , Proteínas Mutantes/fisiologia , Ligação Proteica , Domínios e Motivos de Interação entre Proteínas/genética , Estrutura Secundária de Proteína/genética , Proteína 1 Modificadora da Atividade de Receptores/química , Proteína 1 Modificadora da Atividade de Receptores/metabolismo
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