RESUMO
Orthodontically induced inflammatory root resorption (OIIRR) is considered an undesired and inevitable complication induced by orthodontic forces. This inflammatory mechanism is regulated by immune cells that precede orthodontic tooth movement (OTM) and can influence the severity of OIIRR. The process of OIIRR is based on an immune response. On some occasions, the immune system attacks the dentition by inflammatory processes during orthodontic treatment. Studies on the involvement of the PD-1/PD-L1 immune checkpoint have demonstrated its role in evading immune responses, aiming to identify possible novel therapeutic approaches for periodontitis. In the field of orthodontics, the important question arises of whether PD-L1 has a role in the development of OIIRR to amplify the amount of resorption. We hypothesize that blocking of the PD-L1 immune checkpoint could be a suitable procedure to reduce the process of OIIRR during orthodontic tooth movement. This review attempts to shed light on the regulation of immune mechanisms and inflammatory responses that could influence the pathogenesis of OIIRR and to acquire knowledge about the role of PD-L1 in the immunomodulation involved in OIIRR. Possible clinical outcomes will be discussed in relation to PD-L1 expression and immunologic changes throughout the resorption process.
Assuntos
Antígeno B7-H1 , Reabsorção da Raiz , Técnicas de Movimentação Dentária , Antígeno B7-H1/imunologia , Humanos , Fatores Imunológicos , Receptor de Morte Celular Programada 1 , Reabsorção da Raiz/etiologia , Reabsorção da Raiz/imunologia , Técnicas de Movimentação Dentária/efeitos adversos , Técnicas de Movimentação Dentária/métodosRESUMO
Patients with periodontitis undergoing orthodontic therapy may suffer from undesired dental root resorption. The purpose of this in vitro study was to investigate the molecular mechanisms resulting in PD-L1 expression of cementoblasts in response to infection with Porphyromonas gingivalis (P. gingivalis) peptidoglycan (PGN) and compressive force (CF), and its interaction with hypoxia-inducible factor (HIF)-1α molecule: The cementoblast (OCCM-30) cells were kinetically infected with various concentrations of P. gingivalis PGN in the presence and absence of CF. Western blotting and RT-qPCR were performed to examine the protein expression of PD-L1 and HIF-1α as well as their gene expression. Immunofluorescence was applied to visualize the localization of these proteins within cells. An HIF-1α inhibitor was added for further investigation of necroptosis by flow cytometry analysis. Releases of soluble GAS-6 were measured by ELISA. P. gingivalis PGN dose dependently stimulated PD-L1 upregulation in cementoblasts at protein and mRNA levels. CF combined with P. gingivalis PGN had synergistic effects on the induction of PD-L1. Blockade of HIF-1α inhibited the P. gingivalis PGN-inducible PD-L1 protein expression under compression, indicating an HIF-1α dependent regulation of PD-L1 induction. Concomitantly, an HIF-1α inhibitor decreased the GAS-6 release in the presence of CF and P. gingivalis PGN co-stimulation. The data suggest that PGN of P. gingivalis participates in PD-L1 up-regulation in cementoblasts. Additionally, the influence of compressive force on P. gingivalis PGN-induced PD-L1 expression occurs in HIF-1α dependently. In this regard, HIF-1α may play roles in the immune response of cementoblasts via immune-inhibitory PD-L1. Our results underline the importance of molecular mechanisms involved in bacteria-induced periodontics and root resorption.
Assuntos
Antígeno B7-H1 , Reabsorção da Raiz , Antígeno B7-H1/biossíntese , Antígeno B7-H1/genética , Antígeno B7-H1/imunologia , Cemento Dentário/imunologia , Humanos , Subunidade alfa do Fator 1 Induzível por Hipóxia/genética , Subunidade alfa do Fator 1 Induzível por Hipóxia/imunologia , Peptidoglicano/imunologia , Porphyromonas gingivalis/metabolismo , Reabsorção da Raiz/genética , Reabsorção da Raiz/imunologiaRESUMO
BACKGROUND: MicroRNAs (miRNAs) are involved in the regulation of osteoclast biology and several pathogenic progression. This study aimed to identify the role of miR-26a in osteoclastogenesis and orthodontically induced inflammatory root resorption(OIIRR). METHODS: Rat orthodontic tooth movement (OTM) model was established by ligating a closed coil spring between maxillary first molar and incisor, and 50 g orthodontic force was applied to move upper first molar to middle for 7 days. Human periodontal ligament (hPDL) cells were isolated from periodontium of healthy donors, and then subjected to compression force (CF) for 24 h to mimic an in vitro OTM model. The levels of associated factors in vivo and in vitro were measured subsequently. RESULT: The distance of tooth movement was increased and root resorption pits were occurred in rat OTM model. The expression of miR-26a was decreased in vivo and vitro experiments. CF treatment enhanced the secretion of inflammatory factors receptor activator of nuclear factor-kappa B ligand (RANKL) and IL-6, osteoclast marker levels, and the number of tartrate-resistant acid phosphatase (TRAP)-positive osteoclasts, while miR-26a overexpression reversed these results. Furthermore, miR-26a overexpression inhibited the osteoclastogenesis and rescued the root resorption in OTM rats through inhibition of Jagged1. Additionally, Runx1 could bind to miR-26a promoter and promote its expression, thereby suppressing the osteoclastogenesis. CONCLUSION: We concluded that Runx1/miR-26a/Jagged1 signaling axis restrained osteoclastogenesis and alleviated OIIRR.
Assuntos
Subunidade alfa 2 de Fator de Ligação ao Core/metabolismo , MicroRNAs/genética , MicroRNAs/metabolismo , Osteogênese/genética , Reabsorção da Raiz/imunologia , Técnicas de Movimentação Dentária/efeitos adversos , Adolescente , Adulto , Animais , Células Cultivadas , Modelos Animais de Doenças , Regulação para Baixo/imunologia , Feminino , Humanos , Proteína Jagged-1/genética , Masculino , Osteoclastos , Osteogênese/imunologia , Ligamento Periodontal/citologia , Ligamento Periodontal/patologia , Cultura Primária de Células , Regiões Promotoras Genéticas/genética , Ratos , Reabsorção da Raiz/genética , Reabsorção da Raiz/patologia , Regulação para Cima/imunologia , Adulto JovemRESUMO
AIM: To evaluate the expression of TNF-α, IL-6, IFN-γ, TGF-ß, IL-4, IL-10, RANKL, RANK and OPG on mouse calvarial bone treated with MTA, Geristore® and Emdogain® . METHODOLOGY: Bone wounds were made on the heads of C57BL/6 mice, breaking the periosteum and the cortical surface of the calvaria. Each repair agent was inserted into sectioned Eppendorf microtubes and placed on the bone wound, and soft tissues were sutured. At 14 and 21 days, animals were sacrificed and the treated region was dissected. The calvaria bone was removed, and RNA was extracted. mRNA expression of the aforementioned cytokines was assessed using real-time PCR. Data were analysed by nonparametric methods, including the Mann-Whitney and Kruskal-Wallis tests (P < 0.05). RESULTS: Following treatment with Emdogain® and MTA, mRNA expression of RANKL, RANK and OPG increased significantly (P < 0.05) between days 14 to 21. Geristore® did not alter the basal expression of these mediators during the same period of evaluation. Whilst treatment with Emdogain® did cause a significant increase in TNF-α mRNA expression between days 14 and 21 (P < 0.05), treatment with MTA did not alter the basal expression of this cytokine at either experimental time point. However, TNF-α mRNA expression was down-regulated significantly at day 21 (P < 0.05) when Geristore® was applied. A significant increase in the mRNA expression of IL-6, TGF-ß, IL-10, IL-4 and IFN-γ was observed with Emdogain® and MTA treatment between days 14 to 21, whereas Geristore® reduced significantly the expression of IL-6, TGF-ß and IL-4 (P < 0.05). CONCLUSION: The clinical indication of these repair agents depends on the root resorption diagnosis. Whilst MTA and Emdogain® induce a pro- and anti-inflammatory response early and late, respectively, Geristore® was not associated with an inflammatory reaction when compared with both repair agents.
Assuntos
Compostos de Alumínio/farmacologia , Compostos de Cálcio/farmacologia , Citocinas/metabolismo , Proteínas do Esmalte Dentário/farmacologia , Regulação da Expressão Gênica/efeitos dos fármacos , Cimentos de Ionômeros de Vidro/farmacologia , Óxidos/farmacologia , Resinas Sintéticas/farmacologia , Reabsorção da Raiz/imunologia , Silicatos/farmacologia , Animais , Citocinas/genética , Combinação de Medicamentos , Inflamação/metabolismo , Interferon gama/metabolismo , Interleucina-10/metabolismo , Interleucina-4/metabolismo , Interleucina-6/metabolismo , Camundongos , Camundongos Endogâmicos C57BL , Osteoprotegerina/metabolismo , Ligante RANK/metabolismo , RNA Mensageiro/metabolismo , Receptor Ativador de Fator Nuclear kappa-B/metabolismo , Fator de Crescimento Transformador beta/metabolismo , Fator de Necrose Tumoral alfa/metabolismoRESUMO
INTRODUCTION: The objectives of this study were (1) to investigate the expressions of interleukin (IL)-17, RANKL (the receptor activator of NF-kappaB ligand), and osteoprotegerin (OPG) in root resorption areas during experimental tooth movement in rats, and (2) to determine the effect of IL-17 on the expressions of RANKL and OPG mRNA from human dental pulp cells. METHODS: Twelve male 6-week-old Wistar rats were subjected to an orthodontic force of 50 g to induce a mesially tipping movement of the maxillary first molars for 7 days. The expression levels of tartrate resistant acid phosphatase (TRAP), interleukin (IL)-17, IL-17 receptor (IL-17R), receptor activator of nuclear factor-kappa B ligand (RANKL), and OPG proteins were determined in dental pulp by immunohistochemical analysis. Furthermore, the effects of IL-17 on the expressions of RANKL and OPG mRNA were investigated using human dental pulp cells in vitro. RESULTS: In the experimental tooth movements in vivo, resorption lacunae with multinucleated cells were observed in the 50-g group. The immunoreactivities for IL-17, IL-17R, and RANKL were detected in dental pulp tissues subjected to the orthodontic force on day 7. Moreover, IL-17 increased the mRNA expression of RANKL from human dental pulp cells in vitro. CONCLUSIONS: The results of this study suggest that IL-17 and RANKL may be involved in the process of orthodontically induced inflammatory root resorption in dental pulp cells.
Assuntos
Interleucina-17/análise , Osteoprotegerina/análise , Ligante RANK/análise , Reabsorção da Raiz/imunologia , Técnicas de Movimentação Dentária , Fosfatase Ácida/análise , Adolescente , Animais , Técnicas de Cultura de Células , Células Cultivadas , Polpa Dentária/efeitos dos fármacos , Feminino , Humanos , Interleucina-17/farmacologia , Isoenzimas/análise , Masculino , Osteoclastos/patologia , Osteoprotegerina/efeitos dos fármacos , Ligante RANK/efeitos dos fármacos , Ratos , Ratos Wistar , Receptores de Interleucina-17/análise , Reabsorção da Raiz/patologia , Estresse Mecânico , Fosfatase Ácida Resistente a TartaratoRESUMO
Anti-dentin autoantibodies are associated with inflammatory root resorption in permanent teeth and are modulated by dental trauma and orthodontic force. However, it is not known whether deciduous tooth trauma can stimulate the development of a humoral immune response against dentin. The aim of this study was to evaluate the levels of salivary SIgA reactivity against human dentin extract in young adults with a history of trauma in the primary dentition. A sample of 78 patients, aged 18 to 25, who had completed an early childhood (0 to 5 years old) caries prevention program years earlier at the Universidade Estadual de Londrina Pediatric Clinic, underwent radiographic examination and salivary sampling. Anti-dentin SIgA levels were analyzed by immunoenzymatic assay and Western blotting. Although dental trauma to deciduous teeth had occurred in 34 (43.6%) of the patients, no differences in SIgA levels were detected between individuals who had experienced trauma and those who had not (p > 0.05). Multivariate regression analysis showed no association between dental trauma and SIgA levels (p > 0.05). Patients with a history of deciduous trauma presented low levels of anti-dentin antibodies, associated with orthodontic root resorption (p < 0.05). Western blot analysis showed that salivary antibodies recognized a single band of approximately 45 kDa in dentin extract. We concluded that salivary SIgA recognizes a specific component of the dentin matrix and that anti-dentin antibodies were not triggered by trauma to primary teeth. However, trauma to deciduous teeth may down-modulate SIgA in response to orthodontic root response.
Assuntos
Dentina/imunologia , Imunoglobulina A Secretora/imunologia , Reabsorção da Raiz/imunologia , Reabsorção de Dente , Dente Decíduo/imunologia , Adolescente , Adulto , Pré-Escolar , Dentina/lesões , Feminino , Humanos , Imunoglobulina A Secretora/análise , Lactente , Recém-Nascido , Masculino , Reabsorção da Raiz/etiologia , Saliva/imunologia , Dente Decíduo/lesões , Adulto JovemRESUMO
This retrospective study evaluated the frequency of development of root resorption in dental trauma cases involving supporting tissue. For 249 traumatized teeth of 125 patients aged between 7 and 51 years, we collected data on the gender and age of the patient, the teeth involved, the type of trauma, and the period between dental injury and initial examination. Radiographic parameters examined in relation to root resorption included the presence of inflammatory external root resorption, internal root resorption, replacement resorption, and canal calcification. Data were analyzed by chi-squared test, Fisher's exact test, and mult iple logistic regression (P < 0.05). The results indicated that there was a significant relationship between the period from the date of injury until initial examination and the occurrence of inflammatory external resorption (P = 0.0199), as well as the type of injury (P = 0.0406). Furthermore, external resorption was most frequently associated with intrusive luxation (92.8%), followed by avulsion (89.0%), lateral luxation (80.2%), and extrusive luxation (77.4%). Among the types of dental injury, replacement resorption was observed more frequently in cases of avulsion (87.2%). The only factor that was significantly associated with this type of resorption was the type of injury (P < 0.0001). Root resorption is observed more frequently and its risk of development is higher in cases of severe trauma, especially avulsion and intrusive luxation.
Assuntos
Reabsorção da Raiz/etiologia , Traumatismos Dentários/complicações , Adolescente , Adulto , Criança , Dentição Permanente , Feminino , Humanos , Masculino , Pessoa de Meia-Idade , Estudos Retrospectivos , Reabsorção da Raiz/imunologia , Dente , Traumatismos Dentários/imunologia , Adulto JovemRESUMO
Anti-dentin autoantibodies are associated with inflammatory root resorption in permanent teeth and are modulated by dental trauma and orthodontic force. However, it is not known whether deciduous tooth trauma can stimulate the development of a humoral immune response against dentin. The aim of this study was to evaluate the levels of salivary SIgA reactivity against human dentin extract in young adults with a history of trauma in the primary dentition. A sample of 78 patients, aged 18 to 25, who had completed an early childhood (0 to 5 years old) caries prevention program years earlier at the Universidade Estadual de LondrinaPediatric Clinic, underwent radiographic examination and salivary sampling. Anti-dentin SIgA levels were analyzed by immunoenzymatic assay and Western blotting. Although dental trauma to deciduous teeth had occurred in 34 (43.6%) of the patients, no differences in SIgA levels were detected between individuals who had experienced trauma and those who had not (p > 0.05). Multivariate regression analysis showed no association between dental trauma and SIgA levels (p > 0.05). Patients with a history of deciduous trauma presented low levels of anti-dentin antibodies, associated with orthodontic root resorption (p < 0.05). Western blot analysis showed that salivary antibodies recognized a single band of approximately 45 kDa in dentin extract. We concluded that salivary SIgA recognizes a specific component of the dentin matrix and that anti-dentin antibodies were not triggered by trauma to primary teeth. However, trauma to deciduous teeth may down-modulate SIgA in response to orthodontic root response.
Assuntos
Adolescente , Adulto , Pré-Escolar , Feminino , Humanos , Lactente , Recém-Nascido , Masculino , Adulto Jovem , Dentina/imunologia , Imunoglobulina A Secretora/imunologia , Reabsorção da Raiz/imunologia , Reabsorção de Dente , Dente Decíduo/imunologia , Dentina/lesões , Imunoglobulina A Secretora/análise , Reabsorção da Raiz/etiologia , Saliva/imunologia , Dente Decíduo/lesõesRESUMO
In orthodontic tooth movement (OTM), we should be concerned about external root resorption (ERR) as an undesirable iatrogenic problem, but its mechanisms are not fully understood. Since our previous epidemiologic studies found that patients with allergic diseases showed higher rates of ERR during orthodontic treatment, we explored the possible effect of allergic sensitization on ERR. In ovalbumin (OVA)-sensitized Brown-Norway rats, the amounts of ERR and OTM were greater than those in animals subjected to orthodontic force alone. The expression levels of RANKL and pro-inflammatory cytokines were increased in the periodontal tissues of sensitized rats with OTM, compared with control rats. Furthermore, leukotriene B4 (LTB4), a potent lipid mediator of allergic inflammation, and enzymes of the 5-lipoxygenase pathway, the biosynthetic pathway of leukotrienes, were also up-regulated. We found that low doses of aspirin suppressed ERR in allergen-sensitized rats, as well as the expressions of RANKL, pro-inflammatory cytokines, and LTB4. The present findings indicate that allergen sensitization has adverse effects on ERR under OTM, and that aspirin is a potential therapeutic agent for combating ERR.
Assuntos
Alérgenos/imunologia , Imunização , Reabsorção da Raiz/imunologia , Processo Alveolar/imunologia , Processo Alveolar/patologia , Animais , Araquidonato 5-Lipoxigenase/análise , Aspirina/farmacologia , Fenômenos Biomecânicos , Reabsorção Óssea/imunologia , Reabsorção Óssea/patologia , Inibidores de Ciclo-Oxigenase/farmacologia , Epóxido Hidrolases/análise , Doença Iatrogênica , Imunoglobulina E/sangue , Mediadores da Inflamação/análise , Interleucina-1beta/análise , Interleucina-1beta/efeitos dos fármacos , Interleucina-6/análise , Leucotrieno B4/análise , Leucotrienos/análise , Fios Ortodônticos , Ovalbumina/imunologia , Periodonto/imunologia , Ligante RANK/análise , Ligante RANK/efeitos dos fármacos , Ratos , Ratos Endogâmicos BN , Reabsorção da Raiz/prevenção & controle , Técnicas de Movimentação Dentária/efeitos adversos , Técnicas de Movimentação Dentária/instrumentação , Fator de Necrose Tumoral alfa/análise , Fator de Necrose Tumoral alfa/efeitos dos fármacos , Regulação para CimaRESUMO
OBJECTIVE: The aim of this study was to investigate how atopic dermatitis (AD) contributes to root resorption during orthodontic tooth movement. MATERIALS AND METHODS: Atopic dermatitis model mice and wild-type mice were subjected to an excessive orthodontic force (OF) to induce movement of the upper first molars. The expression levels of the tartrate-resistant acid phosphatase (TRAP), IL-17, IL-6, and RANKL proteins were determined in the periodontal ligament (PDL) by an immunohistochemical analysis. Furthermore, the effects of the compression force on co-cultures of CD4(+) cells from AD patients or healthy individuals and human PDL cells were investigated with regard to the levels of secretion and mRNA expression of IL-17, IL-6, RANKL, and osteoprotegerin. RESULTS: The immunoreactivities for TRAP, IL-17, IL-6, and RANKL in the AD group were found to be significantly increased. The double immunofluorescence analysis for IL-17/CD4 detected immunoreaction. The secretion of IL-17, IL-6, and RANKL, and the mRNA levels of IL-6 and RANKL in the AD patients were increased compared with those in healthy individuals. CONCLUSION: Th17 cells may therefore be associated with the deterioration of root resorption of AD mice, and may explain why AD patients are more susceptible to root resorption than healthy individuals when an excessive OF is applied.
Assuntos
Dermatite Atópica/imunologia , Reabsorção da Raiz/imunologia , Células Th17/imunologia , Técnicas de Movimentação Dentária , Fosfatase Ácida/análise , Adulto , Animais , Linfócitos T CD4-Positivos/imunologia , Técnicas de Cultura de Células , Técnicas de Cocultura , Dermatite Atópica/patologia , Modelos Animais de Doenças , Feminino , Humanos , Imunoglobulina E/sangue , Interleucina-17/análise , Interleucina-17/sangue , Interleucina-6/análise , Isoenzimas/análise , Masculino , Camundongos , Camundongos Endogâmicos BALB C , Osteoprotegerina/análise , Ligamento Periodontal/imunologia , Ligamento Periodontal/patologia , Ligante RANK/análise , Reabsorção da Raiz/patologia , Estresse Mecânico , Fosfatase Ácida Resistente a Tartarato , Técnicas de Movimentação Dentária/efeitos adversos , Adulto JovemRESUMO
BACKGROUND AND OBJECTIVE: Orthodontic tooth movement is known to cause sterile inflammation of the periodontal ligament (PDL). It may also be accompanied by pathological effects of external apical root resorption, with interindividual differences in the incidence and extent of resorption. An involvement of autoimmunological mechanisms is currently under discussion. This study aimed to improve our understanding of similarities between the inflammatory mechanisms underlying the pathophysiology of periodontitis and root resorption. MATERIALS AND METHODS: Human PDL cells were stimulated with interleukin (IL)-1ß/IL-17A/IFN-γ, or left non-stimulated. Their potential for phagocytosis was then evaluated by incubation with dextran or E. coli or S. aureus particles, followed by flow cytometric and immunohistochemical analysis. Real-time polymerase chain reaction (PCR) was used to analyze receptor activator of NF-κB ligand (RANKL) and osteoprotegerin (OPG) expression in PDL cells. Verification was obtained in vivo by studying IL-17A, RANKL, and OPG expression in biopsies of inflamed periodontal tissues and in biopsies of rat maxillae with mechanically induced root resorption. Statistical analysis included Wilcoxon's rank sum test to analyze gene expression data and one-way ANOVA in conjunction with Tukey's post hoc test to analyze flow cytometric data. RESULTS: PDL cells phagocytosed foreign particles under both inflammatory and non-inflammatory conditions. Furthermore, IL-17A significantly downregulated RANKL expression while significantly upregulating OPG expression in PDL cells. These immunomodulatory cytokines were also demonstrable in both inflammatorily altered periodontal tissues and root resorption lacunae, while the incidence of IL-7A was strikingly variable in resorption areas. CONCLUSION: PDL cells were demonstrated to effect phagocytosis and to express immunomodulatory molecules, which proves their capability of participating in periodontal osteoimmunological processes. The development of root resorption and periodontitis appears to be governed by similar pathophysiological mechanisms.
Assuntos
Infecções Bacterianas/imunologia , Citocinas/imunologia , Ligamento Periodontal/imunologia , Periodontite/imunologia , Fagocitose/imunologia , Reabsorção da Raiz/imunologia , Técnicas de Movimentação Dentária/efeitos adversos , Adolescente , Células Cultivadas , Feminino , Humanos , Masculino , Ligamento Periodontal/patologia , Reabsorção da Raiz/patologia , Estresse Fisiológico/imunologiaRESUMO
OBJECTIVE: The aim of this study was to investigate how T-helper 17 cells (Th17 cells), interleukin (IL)-17, and interleukin-6 contribute to root resorption during orthodontic tooth movement. MATERIALS AND METHODS: Fifteen male 6-week-old Wistar rats were subjected to orthodontic force of 10 or 50 g to induce a mesially tipping movement of the upper first molars for 7 days. The expression levels of TRAP, IL-17, the IL-17 receptor (IL-17R), and IL-6 proteins were determined in periodontal ligament (PDL) by immunohistochemical analysis. Moreover, the fluorescent localization immunoassay was performed to detect Th17 cells. Furthermore, the effects of IL-17 on IL-6 release were investigated using human PDL cells in vitro. The effect of IL-17 on osteoclastogenesis was evaluated by TRAP staining, actin ring staining, and the pit formation assay. RESULTS: The immunoreactivity for Th17, IL-17, IL-17R, and IL-6 was detected in PDL tissue subjected to the orthodontic force on day 7. IL-17 increased the release of IL-6 from human periodontal ligament cells in a time-dependent manner. Moreover, IL-17 stimulated osteoclastogenesis from human osteoclast precursor cells, and these effects were partially suppressed by an anti-IL-6 antibody. CONCLUSION: These results suggest that Th17 cells may aggravate the process of orthodontically induced inflammatory root resorption.
Assuntos
Osteoclastos/imunologia , Células Th17/imunologia , Técnicas de Movimentação Dentária/métodos , Fosfatase Ácida/análise , Actinas/análise , Adolescente , Animais , Biomarcadores/análise , Fenômenos Biomecânicos , Técnicas de Cultura de Células , Linhagem Celular , Tecido Conjuntivo/patologia , Dentina/patologia , Relação Dose-Resposta a Droga , Feminino , Fibroblastos/patologia , Humanos , Interleucina-17/análise , Interleucina-17/farmacologia , Interleucina-6/análise , Isoenzimas/análise , Masculino , Dente Molar/patologia , Fios Ortodônticos , Osteoclastos/efeitos dos fármacos , Ligamento Periodontal/efeitos dos fármacos , Ligamento Periodontal/patologia , Ratos , Ratos Wistar , Receptores de Interleucina-17/análise , Reabsorção da Raiz/imunologia , Reabsorção da Raiz/patologia , Estresse Mecânico , Fosfatase Ácida Resistente a Tartarato , Fatores de Tempo , Técnicas de Movimentação Dentária/instrumentaçãoRESUMO
BACKGROUND AND OBJECTIVE: A therapeutic protocol to minimize root resorption induced by tooth replantation has not yet been universally established. In this context, noninvasive modality such as ultrasound therapy have been a focus of increased interest. This study aimed to evaluate the inhibitory effect of ultrasound therapy on root resorption of replanted rat molars. In addition, the study aimed to promote insights into the mechanism through which ultrasound mediates the metabolism of periodontal cells in vitro. MATERIAL AND METHODS: An experimental model of tooth replantation in rats, involving luxation and immediate replacement of the maxillary first molars, was used to assess the inhibitory effect of an ultrasound-therapy regimen (15 min of exposure to ultrasound, each day for 21 d) on root resorption. Moreover, the effect of ultrasound on osteoclastogenesis/cementoclastogenesis was examined in vitro using a mouse osteoblastic stromal cell line (ST2) and a mouse cementoblastic cell line (OCCM-30). RESULTS: The area of root resorption lacunae was statistically decreased (p < 0.01) in the ultrasound-treated sample. In addition, immunohistochemical staining, using murine TNF-α polyclonal antibody, failed to detect tumor necrosis factor-α (TNF-α) protein in the ultrasound-treated sample compared with the control. An in vitro study showed that the lipopolysaccharide (LPS)-induced expression of Tnfalpha mRNA was significantly reduced by ultrasound therapy in both osteoblastic and cementoblastic cells. Moreover, the TNF-α-induced up-regulation of Rankl mRNA was also inhibited by ultrasound. CONCLUSION: Ultrasound may contribute to the reduction of the trauma-induced inflammatory reaction through impairment of the TNF-α signaling pathway. It is therefore suggested that ultrasound shows potential as a therapeutic tool to optimize the regenerative potential of periodontal tissues on replanted teeth.
Assuntos
Reabsorção da Raiz/prevenção & controle , Transdução de Sinais , Reimplante Dentário/efeitos adversos , Fator de Necrose Tumoral alfa/fisiologia , Terapia por Ultrassom , Animais , Linhagem Celular , Cemento Dentário/citologia , Lipopolissacarídeos/farmacologia , Masculino , Dente Molar/cirurgia , Osteoblastos , Osteoclastos , Ligamento Periodontal/fisiologia , Ligante RANK/antagonistas & inibidores , Ratos , Ratos Wistar , Proteínas Recombinantes/farmacologia , Regeneração/fisiologia , Reabsorção da Raiz/etiologia , Reabsorção da Raiz/imunologia , Células Estromais , Fator de Necrose Tumoral alfa/antagonistas & inibidores , Fator de Necrose Tumoral alfa/biossínteseRESUMO
The aim of this study was to analyse serum IgG levels and salivary secretory IgA (sIgA) levels in human dentine extract (HDE) before (T0) and 6 months after (T6) orthodontic treatment and to correlate anti-HDE autoantibodies to root resorption. Fifty orthodontic patients were selected, 19 males (15.6 ± 8.5 years) and 31 females (21.4 ± 11.2 years), 19 in the mixed dentition (10.3 ± 1.9 years) and 31 in the permanent dentition (24.6 ± 9.9 years). Fifty individuals not undergoing orthodontic treatment matched by gender and age were selected as the controls. Periapical radiographs of the upper central incisors and saliva sampling were obtained of all patients at T0 and T6. Serum samples were collected from the permanent dentition patients (n = 31). Antibody levels were determined by means of immunoenzyme assay. At T6, root resorption was classified as grade 0 (no resorption), grade 1 (slight resorption), and grade 2 (moderate to severe resorption). Differences between antibody levels at T0 and T6 and among different grades of resorption were determined by paired t- and Kruskal-Wallis tests, respectively. Spearman's rank correlation coefficient was applied to detect correlation between sIgA and IgG levels, and logistic regression to determine the association of root resorption grade and the studied variables. Differences were considered significant at P < 0.05. Serum anti-HDE IgG levels decreased (P < 0.01) in grade 2 root resorption patients during treatment and was not correlated to salivary sIgA levels or other variables. Patients who had grade 2 root resorption at T6 showed higher levels of anti-HDE sIgA (P < 0.001). Anti-HDE sIgA levels at T0 and root shape were the main factors associated with the degree of root resorption. The results suggest that variations to systemic and local humoural immune response to dentine antigens may occur during orthodontic treatment. High levels of salivary sIgA before treatment were associated with more advanced lesions after 6 months of treatment.
Assuntos
Dentina/imunologia , Ortodontia Corretiva/efeitos adversos , Reabsorção da Raiz/imunologia , Adolescente , Adulto , Análise de Variância , Estudos de Casos e Controles , Criança , Feminino , Humanos , Imunoglobulina A/sangue , Imunoglobulina G/sangue , Masculino , Valores de Referência , Reabsorção da Raiz/etiologia , Saliva/imunologia , Adulto JovemRESUMO
BACKGROUND: The aim of this in vivo study was to evaluate the correlation between the occurrence of root resorption in avulsed and replanted teeth, extra-alveolar time, and the possible influence of Th2 immunologic profile, represented by patient's atopy and its influence in the prognosis of the tooth. METHODS: This study evaluated the presence or absence of root resorption in 57 avulsed and replanted teeth. The replantation technique and endodontic treatments were performed following the International Association of Dental Trauma guidelines. Follow-up of the replanted teeth included monthly clinical and radiographic examinations during 1 year. The patient's medical examination, including the personal and family allergic history, in conjunction with the skin prick test based the evaluation of patient's atopy. RESULTS: Of the 37 teeth of atopic patients, 33 (89.2%) teeth did not present root resorption. Of these, 27 (72.9%) had an extra-alveolar time of <1 hour and 6 (16.2%) had an extra-alveolar time >1 hour. Four (10.8%) teeth presented root resorption and an extra-alveolar period of time >1 hour. CONCLUSION: These results showed the importance of the extra-alveolar time and the predominantly Th2 immunologic profile, represented by atopic patients, that suffered replantation after dental avulsion in a 1-year follow-up.
Assuntos
Avulsão Dentária/cirurgia , Reimplante Dentário/métodos , Distribuição de Qui-Quadrado , Humanos , Prognóstico , Radiografia , Reabsorção da Raiz/diagnóstico por imagem , Reabsorção da Raiz/imunologia , Reabsorção da Raiz/cirurgia , Testes Cutâneos , Fatores de Tempo , Avulsão Dentária/diagnóstico por imagem , Avulsão Dentária/imunologia , Resultado do TratamentoRESUMO
Previous studies have reported changes both in dental pulp and in periodontal ligament (PDL) following orthodontic tooth movement. However, pulpal changes following extensive root resorption after orthodontic tooth movement have not been studied in detail. The aim of this study was therefore to evaluate inflammatory changes, both in the dental pulp and in the compressed PDL, after experimentally induced extensive root resorption. Extensive root resorption was induced in rats by the activation and re-activation of orthodontic force, with a short intervening period of no force application. The distribution of immune cells, nerve fibres and blood vessels was studied immunohistochemically using antibodies against CD68-immunoreactive (IR) cells, major histocompatibility complex (MHC) class II Ia-expressing cells, CD43-IR cells, protein gene product 9.5 (PGP 9.5), and laminin. In the compressed PDL of experimental first molars, significantly increased density of CD68-IR cells and MHC class II Ia-expressing cells were found, whereas the density of CD43-IR cells were unchanged when compared with control second molars. In the compressed PDL, there was an increased density of blood vessels, but no sprouting of nerve fibres. In the dental pulp, however, no increased density of immune cells or sprouting of nerve fibres was recorded. In conclusion, inflammation after extensive root resorption was confined to the compressed PDL, whereas the dental pulp was unaffected.
Assuntos
Polpa Dentária/patologia , Ligamento Periodontal/patologia , Periodontite/etiologia , Pulpite/etiologia , Reabsorção da Raiz/etiologia , Técnicas de Movimentação Dentária/efeitos adversos , Animais , Antígenos CD/análise , Antígenos de Diferenciação Mielomonocítica/análise , Polpa Dentária/imunologia , Antígenos de Histocompatibilidade Classe II/análise , Laminina/análise , Leucócitos Mononucleares/patologia , Leucossialina/análise , Macrófagos/patologia , Masculino , Microvasos/patologia , Dente Molar/imunologia , Dente Molar/patologia , Fibras Nervosas/patologia , Ligamento Periodontal/imunologia , Periodontite/imunologia , Periodontite/patologia , Pressão , Pulpite/imunologia , Pulpite/patologia , Ratos , Ratos Sprague-Dawley , Reabsorção da Raiz/imunologia , Reabsorção da Raiz/patologia , Linfócitos T/patologia , Ubiquitina Tiolesterase/análiseRESUMO
BACKGROUND: Replantation is the most indicated procedure for traumatic dental avulsion, but it invariably results in a greater or lower degree of root resorption. The outcomes of these cases can be affected by some well-known factors. Because studies have shown the importance of the innate and acquired immune response in the mechanisms involved in the resorption of bone tissues, an endeavor was made to verify if such an influence applies to dental resorption. METHODS: Fifty-seven avulsed, endodontically treated teeth were assessed in the Endodontics Clinic, Pontifical Catholic University of Paraná. Follow-up of the replanted teeth included periodical clinical examinations and radiographs, in accordance with the control rules of the International Association of Dental Traumatology. The evaluation of atopy was based on the patient's personal and family history in conjunction with the skin-prick test for five allergen extracts. RESULTS: Of the 46 teeth with a favorable outcome, 33 (71.74%) were in atopic patients, and 13 (28.26%) were in non-atopic patients. Of the 11 teeth with an unfavorable outcome, four (36.36%) were in atopic patients, and seven (63.64%) were in non-atopic patients; a greater prevalence of unfavorable prognoses occurred in non-atopic patients. CONCLUSION: The outcome after 1 year for avulsed and replanted teeth is more favorable in atopic patients.
Assuntos
Hipersensibilidade/complicações , Imunidade Inata/imunologia , Reabsorção da Raiz/imunologia , Avulsão Dentária/imunologia , Reimplante Dentário , Distribuição de Qui-Quadrado , Criança , Feminino , Seguimentos , Humanos , Hipersensibilidade/diagnóstico , Hipersensibilidade/imunologia , Masculino , Prognóstico , Reabsorção da Raiz/complicações , Testes Cutâneos , Estatísticas não Paramétricas , Avulsão Dentária/terapia , Dente não Vital/imunologia , Dente não Vital/cirurgia , Resultado do Tratamento , Adulto JovemRESUMO
Root resorption seems to be related to a complex combination of mechanical factors and biological activity, which comprehends the role of immunologic structures including specialized cells. The aim of this research was to explain the development of the process--from mineralization to the destruction of hard tissues--and the possible relationship between root resorption and immunology, along with discussing current concepts described in the literature.
Assuntos
Reabsorção da Raiz/imunologia , Animais , Humanos , Imunidade Celular/imunologia , Imunidade Inata/imunologia , Odontogênese/fisiologia , Reabsorção da Raiz/fisiopatologia , Calcificação de Dente/imunologia , Calcificação de Dente/fisiologiaRESUMO
The objective of this study was to determine whether there is an association between excessive root resorption and immune system factors in a sample of Japanese orthodontic patients. The records of 60 orthodontic patients (18 males, age 17.7 +/- 5.7 years; 42 females, age 16.4 +/- 6.0 years) and 60 pair-matched controls (18 males, age 15.9 +/- 4.5 years; 42 females, age 18.5 +/- 5.2 years) based on age, sex, treatment duration, and the type of malocclusion were reviewed retrospectively. The validity of our hypothesis was tested using the logistic regression analysis. The pretreatment records revealed that the incidence of allergy and root morphology abnormality was significantly higher in the root resorption group (P = .030 and .001), with a mean odds ratio of 2.794 and 6.317 and 95% confidence interval of 1.107-7.053 and 2.043-19.537, respectively. The incidence of asthma also tended to be higher in the root resorption group. From these results, we concluded that allergy, root morphology abnormality, and asthma may be high-risk factors for the development of excessive root resorption during orthodontic tooth movement in Japanese patients.
Assuntos
Asma/complicações , Hipersensibilidade/complicações , Reabsorção da Raiz/imunologia , Raiz Dentária/anormalidades , Adolescente , Povo Asiático , Asma/epidemiologia , Métodos Epidemiológicos , Feminino , Humanos , Hipersensibilidade/epidemiologia , Masculino , Ortodontia Corretiva , Reabsorção da Raiz/epidemiologia , Raiz Dentária/imunologiaRESUMO
Replacement dental resorption may be a consequence of trauma and may cause dental transplants or reimplants to fail. Previously, we demonstrated the participation of the immunopathological response in inflammatory dental resorption. The induction mechanisms of the two types of dental resorption are well known to be different. The aim of the present study was to observe the immune response of patients who suffered dental trauma with subsequent replacement dental resorption. Four patients with replacement radicular resorption and four healthy individuals with no evidence of radicular resorption participated in the study. The results of ELISA demonstrated that serum from patients with replacement dental resorption contained larger amounts of IgG and smaller amounts of IgM anti-total human-dentin extract and anti-fractions of extract than did serum from control individuals. These results signal the hypothesis that dentin is immunogenic and the serological profile of patients with replacement dental resorption may be identified through biochemical analysis of their blood. Precise screening by this method may allow early diagnosis of dental resorption before it becomes visible radiographically.