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1.
Invest Ophthalmol Vis Sci ; 62(6): 28, 2021 05 03.
Artigo em Inglês | MEDLINE | ID: mdl-34043748

RESUMO

Purpose: C-type lectin-like receptor-1 (CLEC-1) is a member of the Dectin-1 cluster of pattern recognition receptors (PRRs). It is involved in host immunity, has immunoregulatory function, and supports allograft tolerance. Our study aimed to describe the role of CLEC-1 in response to fungal keratitis, in situ, in vivo, and in vitro. Methods: Quantitative polymerase chain reaction (qRT-PCR) and immunofluorescence were used to detect the expression of CLEC-1 in corneas of patients with Aspergillus fumigatus (A. fumigatus) keratitis. In vitro and in vivo experiments were designed in THP-1 macrophages and C57BL/6 mouse models, respectively. The expression of CLEC-1 in corneas of mice model was determined by qRT-PCR, Western blot, and immunofluorescence. CLEC-1 overexpression in mouse corneas was achieved by intrastromal injection of adeno-associated virus (AAV) vectors. Disease response was evaluated by slit-lamp photography, clinical score, and colony forming unit (CFU). Bioluminescence imaging system image acquisition, myeloperoxidase (MPO) assays, immunofluorescence staining, qRT-PCR, and Western blot were used to investigate the role of CLEC-1. To further define the role of CLEC-1, we used lentivirus vectors to overexpress CLEC-1 or/and Dectin-1 in THP-1 macrophages. Results: The expression of CLEC-1 was increased in corneas of patients with A. fumigatus keratitis. In corneas of mice from the A. fumigatus keratitis model, the expression of CLEC-1 was decreased in the acute inflammatory stage and increased during convalescence. Following Natamycin treatment, CLEC-1 was upregulated in A. fumigatus keratitis mice. Compared with normal C57BL/6 mice, overexpression of CLEC-1 converted the characteristic susceptible response to resistance, as demonstrated by slit-lamp photography and clinical score. In vivo studies revealed decreased MPO levels and neutrophils recruitment and higher fungal load after the upregulation of CLEC-1. Compared with control corneas, CLEC-1 overexpression impaired corneal pro-inflammatory cytokine IL-1ß production. Conclusions: These findings demonstrate that CLEC-1 may act as a negative regulator of Dectin-1 induced host inflammatory response via suppressing neutrophils recruitment and production of pro-inflammatory cytokine IL-1ß production in response to A. fumigatus keratitis.


Assuntos
Aspergilose/metabolismo , Infecções Oculares Fúngicas/metabolismo , Regulação da Expressão Gênica/fisiologia , Ceratite/metabolismo , Lectinas Tipo C/genética , Lectinas Tipo C/fisiologia , Proteínas de Membrana/fisiologia , Animais , Aspergilose/imunologia , Aspergilose/microbiologia , Aspergillus fumigatus , Western Blotting , Citocinas/metabolismo , Dependovirus/genética , Modelos Animais de Doenças , Infecções Oculares Fúngicas/imunologia , Infecções Oculares Fúngicas/microbiologia , Feminino , Vetores Genéticos , Humanos , Ceratite/imunologia , Ceratite/microbiologia , Macrófagos/metabolismo , Camundongos , Camundongos Endogâmicos C57BL , Microscopia de Fluorescência , Infiltração de Neutrófilos , Peroxidase/metabolismo , Reação em Cadeia da Polimerase em Tempo Real , Receptores Mitogênicos/fisiologia , Microscopia com Lâmpada de Fenda
2.
Front Immunol ; 11: 251, 2020.
Artigo em Inglês | MEDLINE | ID: mdl-32133013

RESUMO

C-type lectin-like receptors (CLRs) represent a family of transmembrane pattern recognition receptors, expressed primarily by myeloid cells. They recognize not only pathogen moieties for host defense, but also modified self-antigens such as damage-associated molecular patterns released from dead cells. Upon ligation, CLR signaling leads to the production of inflammatory mediators to shape amplitude, duration and outcome of the immune response. Thus, following excessive injury, dysregulation of these receptors leads to the development of inflammatory diseases. Herein, we will focus on four CLRs of the "Dectin family," shown to decode the immunogenicity of cell death. CLEC9A on dendritic cells links F-actin exposed by dying cells to favor cross-presentation of dead-cell associated antigens to CD8+ T cells. Nevertheless, CLEC9A exerts also feedback mechanisms to temper neutrophil recruitment and prevent additional tissue damage. MINCLE expressed by macrophages binds nuclear SAP130 released by necrotic cells to potentiate pro-inflammatory responses. However, the consequent inflammation can exacerbate pathogenesis of inflammatory diseases. Moreover, in a tumor microenvironment, MINCLE induces macrophage-induced immune suppression and cancer progression. Similarly, triggering of LOX-1 by oxidized LDL, amplifies pro-inflammatory response but promotes tumor immune escape and metastasis. Finally, CLEC12A that recognizes monosodium urate crystals formed during cell death, inhibits activating signals to prevent detrimental inflammation. Interestingly, CLEC12A also sustains type-I IFN response to finely tune immune responses in case of viral-induced collateral damage. Therefore, CLRs acting in concert as sensors of injury, could be used in a targeted way to treat numerous diseases such as allergies, obesity, tumors, and autoimmunity.


Assuntos
Morte Celular/imunologia , Lectinas Tipo C/fisiologia , Animais , Humanos , Receptores Imunológicos/fisiologia , Receptores Mitogênicos/fisiologia , Receptores Depuradores Classe E/fisiologia
3.
Br J Haematol ; 183(2): 185-195, 2018 10.
Artigo em Inglês | MEDLINE | ID: mdl-30378120

RESUMO

The mechanisms involved in regulating von Willebrand factor (VWF) clearance remain poorly understood. However recent studies have shown that macrophages play a critical role in regulating the half-life of VWF, and have identified specific lectin (including asialoglycoprotein, macrophage galactose-type lectin, Sigec-5 and C-type lectin domain family 4 member M) and scavenger receptors (including low-density lipoprotein receptor-related protein-1, scavenger receptor A1 and stabilin-2) that are involved in VWF clearance. Further studies will be required to determine the relative importance of these individual receptors with respect to physiological and pathological VWF clearance. Nevertheless, recent clinical data have highlighted the importance of enhanced VWF clearance in the pathogenesis of type 1 von Willebrand disease (VWD). Moreover, increased clearance also contributes to reduced VWF levels in many patients with type 2 and type 3 VWD. Improved understanding regarding VWF clearance is not only of direct biological relevance, but may also have important implications for the development of novel therapeutic agents with extended plasma half-lives for the treatment of both VWD and haemophilia A.


Assuntos
Doenças de von Willebrand/sangue , Fator de von Willebrand/metabolismo , Desamino Arginina Vasopressina/uso terapêutico , Hemostáticos/uso terapêutico , Humanos , Mutação , Receptores Mitogênicos/fisiologia , Receptores Depuradores/fisiologia , Relação Estrutura-Atividade , Doenças de von Willebrand/tratamento farmacológico , Doenças de von Willebrand/etiologia , Fator de von Willebrand/genética
4.
Cancer Lett ; 412: 143-154, 2018 01 01.
Artigo em Inglês | MEDLINE | ID: mdl-29061505

RESUMO

Specific cell populations leading the local invasion of cancer are called "leading cells". However, the underlying mechanisms are unclear. Here, we identified leading cells in pancreatic cancer and determined how these cells lead and promote cancer cell invasion in the extracellular matrix (ECM). Using three-dimensional matrix remodeling assay, we found that pancreatic stellate cells (PSCs) frequently invaded the collagen matrix with pancreatic cancer cells (PCCs), which invaded behind the invading PSCs. In addition, invading PSCs changed the alignment of collagen fibers, resulting in ECM remodeling and an increase in the parallel fibers along the direction of invading PSCs. Endo180 expression was higher in PSCs than in PCCs, Endo180 knockdown in PSCs attenuated the invasive abilities of PSCs and co-cultured PCCs, and decreased the expression level of phosphorylated myosin light chain 2 (MLC2). In mouse models, Endo180-knockdown PSCs suppressed tumor growth and changes in collagen fiber orientation in co-transplantation with PCCs. Our findings suggest that PSCs lead the local invasion of PCCs by physically remodeling the ECM, possibly via the function of Endo180, which reconstructs the actin cell skeleton by phosphorylation of MLC2.


Assuntos
Matriz Extracelular/química , Neoplasias Pancreáticas/patologia , Células Estreladas do Pâncreas/fisiologia , Receptores Mitogênicos/fisiologia , Miosinas Cardíacas/metabolismo , Linhagem Celular Tumoral , Colágeno/química , Humanos , Cadeias Leves de Miosina/metabolismo , Invasividade Neoplásica , Fosforilação
5.
PLoS One ; 12(10): e0186780, 2017.
Artigo em Inglês | MEDLINE | ID: mdl-29065139

RESUMO

Tuberculosis is a fatal human infectious disease caused by Mycobacterium tuberculosis (M. tuberculosis) that is prevalent worldwide. Mycobacteria differ from other bacteria in that they have a cell wall composed of specific surface glycans that are the major determinant of these organisms' pathogenicity. The interaction of M. tuberculosis with pattern recognition receptors (PRRs), in particular C-type lectin receptors (CLRs), on the surface of macrophages plays a central role in initiating innate and adaptive immunity, but the picture as a whole remains a puzzle. Defining novel mechanisms by which host receptors interact with pathogens in order to modulate a specific immune response is an area of intense research. In this study, based on an in vitro lectin binding assay, CLEC9A (DNGR-1) is identified as a novel CLR that binds with mycobacteria. Our results with CLEC9A-knocked down cells and a CLEC9A-Fc fusion protein as blocking agents show that CLEC9A is involved in the activation of SYK and MAPK signaling in response to heat-killed M. tuberculosis H37Ra treatment, and it then promotes the production of CXCL8 and IL-1ß in macrophages. The CXCL8 and IL-1ß secreted by the activated macrophages are critical to neutrophil recruitment and activation. In a in vivo mouse model, when the interaction between CLEC9A and H37Ra is interfered with by treatment with CLEC9A-Fc fusion protein, this reduces lung inflammation and cell infiltration. These findings demonstrate that CLEC9A is a specialized receptor that modulates the innate immune response when there is a mycobacterial infection.


Assuntos
Temperatura Alta , Lectinas Tipo C/fisiologia , Macrófagos/fisiologia , Mycobacterium tuberculosis/fisiologia , Neutrófilos/citologia , Receptores Mitogênicos/fisiologia , Animais , Linhagem Celular , Técnicas de Silenciamento de Genes , Humanos , Lectinas Tipo C/genética , Macrófagos/enzimologia , Masculino , Camundongos , Camundongos Endogâmicos C57BL , Proteínas Quinases/metabolismo , Receptores Mitogênicos/genética , Transdução de Sinais
6.
Ann Rheum Dis ; 75(7): 1386-91, 2016 07.
Artigo em Inglês | MEDLINE | ID: mdl-26275430

RESUMO

BACKGROUND: Myeloid inhibitory C-type lectin-like receptor (MICL, Clec12A) is a C-type lectin receptor (CLR) expressed predominantly by myeloid cells. Previous studies have suggested that MICL is involved in controlling inflammation. OBJECTIVE: To determine the role of this CLR in inflammatory pathology using Clec12A(-/-) mice. METHODS: Clec12A(-/-) mice were generated commercially and primarily characterised using the collagen antibody-induced arthritis (CAIA) model. Mechanisms and progress of disease were characterised by clinical scoring, histology, flow cytometry, irradiation bone-marrow chimera generation, administration of blocking antibodies and in vivo imaging. Characterisation of MICL in patients with rheumatoid arthritis (RA) was determined by immunohistochemistry and single nucleotide polymorphism analysis. Anti-MICL antibodies were detected in patient serum by ELISA and dot-blot analysis. RESULTS: MICL-deficient animals did not present with pan-immune dysfunction, but exhibited markedly exacerbated inflammation during CAIA, owing to the inappropriate activation of myeloid cells. Polymorphisms of MICL were not associated with disease in patients with RA, but this CLR was the target of autoantibodies in a subset of patients with RA. In wild-type mice the administration of such antibodies recapitulated the Clec12A(-/-) phenotype. CONCLUSIONS: MICL plays an essential role in regulating inflammation during arthritis and is an autoantigen in a subset of patients with RA. These data suggest an entirely new mechanism underlying RA pathogenesis, whereby the threshold of myeloid cell activation can be modulated by autoantibodies that bind to cell membrane-expressed inhibitory receptors.


Assuntos
Artrite Experimental/genética , Artrite Reumatoide/genética , Lectinas Tipo C/fisiologia , Receptores Mitogênicos/fisiologia , Animais , Artrite Reumatoide/sangue , Artrite Reumatoide/etiologia , Artrite Reumatoide/patologia , Autoanticorpos/metabolismo , Ensaio de Imunoadsorção Enzimática , Humanos , Lectinas Tipo C/deficiência , Lectinas Tipo C/imunologia , Camundongos , Células Mieloides/metabolismo , Polimorfismo Genético , Receptores Mitogênicos/deficiência , Receptores Mitogênicos/imunologia , Membrana Sinovial/patologia
7.
Biochem Biophys Res Commun ; 464(2): 392-5, 2015 Aug 21.
Artigo em Inglês | MEDLINE | ID: mdl-26067556

RESUMO

The delivery of biologically functional peptides into mammalian cells can be a direct and effective method for cancer therapy and treatment of other diseases. Discoidin domain receptor 2 (DDR2) is a collagen-induced receptor tyrosine kinase recently identified as a novel therapeutic target in lung cancer. In this study, we report that peptides containing the functional domain of DDR2 can be efficiently delivered into lung malignant cancer cells via a gold nanoparticle-DNA aptamer conjugate (AuNP-Apt)-based system. Peptide delivery resulted in the abrogation of DDR2 activation triggered by collagen. Moreover, the peptide delivered by the AuNP-Apt system inhibited cancer cell proliferation and invasion mediated by DDR2 activation. Thus, these results suggest that peptide loaded onto AuNP-Apt conjugates can be used for the development of peptide-based biomedical applications for the treatment of DDR2-positive cancer.


Assuntos
Aptâmeros de Nucleotídeos , Carcinoma Pulmonar de Células não Pequenas/patologia , Ouro/química , Neoplasias Pulmonares/patologia , Nanopartículas Metálicas , Peptídeos/administração & dosagem , Receptores Proteína Tirosina Quinases/antagonistas & inibidores , Receptores Mitogênicos/antagonistas & inibidores , Linhagem Celular Tumoral , Membrana Celular , Receptores com Domínio Discoidina , Humanos , Peptídeos/química , Receptores Proteína Tirosina Quinases/fisiologia , Receptores Mitogênicos/fisiologia
8.
Int J Biochem Cell Biol ; 62: 80-7, 2015 May.
Artigo em Inglês | MEDLINE | ID: mdl-25747904

RESUMO

The anti-transplant rejection drug cyclosporin A (CsA) causes loss of collagen homeostasis in rapidly remodeling connective tissues, such as human gingiva. As a result of CsA treatment, collagen degradation by fibroblasts is inhibited, which leads to a net increase of tissue collagen and gingival overgrowth. Since fibrillar collagen is the primary ligand for the discoidin domain receptor 1 (DDR1), we hypothesized that CsA perturbs DDR1-associated functions that affect collagen homeostasis. For these experiments, human fibroblasts obtained from gingival explants or mouse 3T3 fibroblasts (wild type, over-expressing DDR1 or DDR1 knockdown) or mouse GD25 cells (expressing DDR1 but null for ß1 integrin), were treated with vehicle (dimethyl sulfoxide) or with CsA. The effect of CsA on cell binding to collagen was examined by flow cytometry; cell-mediated collagen remodeling was analyzed with contraction, compaction and migration assays. We found that CsA inhibited cell binding to collagen, internalization of collagen, contraction of collagen gels and cell migration over collagen in a DDR1-dependent manner. CsA also enhanced collagen compaction around cell extensions. Treatment with CsA strongly reduced surface levels of ß1 integrins in wild type and DDR1 over-expressing 3T3 cells but did not affect ß1 integrin activation or focal adhesion formation. We conclude that CsA inhibition of collagen remodeling is mediated through its effects on both DDR1 and cell surface levels of the ß1 integrin.


Assuntos
Colágeno/metabolismo , Ciclosporina/farmacologia , Receptores Proteína Tirosina Quinases/fisiologia , Receptores Mitogênicos/fisiologia , Animais , Movimento Celular/efeitos dos fármacos , Células Cultivadas , Colágeno/efeitos dos fármacos , Receptores com Domínio Discoidina , Fibroblastos/efeitos dos fármacos , Fibroblastos/fisiologia , Humanos , Integrina beta1/metabolismo , Camundongos , Células NIH 3T3 , Ligação Proteica/efeitos dos fármacos
9.
Int J Cancer ; 136(6): E508-20, 2015 Mar 15.
Artigo em Inglês | MEDLINE | ID: mdl-25155634

RESUMO

The epithelial-to-mesenchymal transition (EMT) process allows carcinoma cells to dissociate from the primary tumor thereby facilitating tumor cell invasion and metastasis. Ras-dependent hyperactive signaling is commonly associated with tumorigenesis, invasion, EMT, and metastasis. However, the downstream effectors by which Ras regulates EMT remain ill defined. In this study, we show that the H-Ras pathway leads to mesenchymal-like phenotypic changes in human breast epithelial cells by controlling the ZEB1/microRNA-200c axis. Moreover, H-Ras suppresses the expression of the discoidin domain receptor 1 (DDR1), a collagen receptor tyrosine kinase, via ZEB1, thus identifying ZEB1 as a novel transcriptional repressor of DDR1. Mutation studies on the putative promoter of the DDR1 gene revealed that bipartite Z- and E-box elements play a key role in transcriptional repression of DDR1 in Hs578T and MDA-MB-231 breast carcinoma cell lines by ZEB1. Furthermore, we found an inverse correlation between ZEB1 and DDR1 expression in various cancer cell lines and in human breast carcinoma tissues. Consistently, overexpression of DDR1 reduced the invasive phenotype of mesenchymal-like triple-negative breast cancer cells in 3D cultures and in vivo. Thus, ZEB1's role in maintenance of EMT in breast carcinoma cells is mediated in part by its ability to suppress DDR1 expression and consequently contribute to the activation of the invasive phenotype. Taken together, our results unveil a novel H-Ras/ZEB1/DDR1 network that contributes to breast cancer progression in triple-negative breast cancers.


Assuntos
Mama/patologia , Transição Epitelial-Mesenquimal , Genes ras/fisiologia , Proteínas de Homeodomínio/fisiologia , Receptores Proteína Tirosina Quinases/fisiologia , Receptores Mitogênicos/fisiologia , Fatores de Transcrição/fisiologia , Linhagem Celular Tumoral , Citoesqueleto/fisiologia , Receptores com Domínio Discoidina , Células Epiteliais/patologia , Feminino , Humanos , MicroRNAs/fisiologia , Morfogênese , Homeobox 1 de Ligação a E-box em Dedo de Zinco
10.
Int Rev Cell Mol Biol ; 310: 39-87, 2014.
Artigo em Inglês | MEDLINE | ID: mdl-24725424

RESUMO

The discoidin domain receptors, DDR1 and DDR2, are nonintegrin collagen receptors that are members of the receptor tyrosine kinase family. Both DDRs bind a number of different collagen types and play important roles in embryo development. Dysregulated DDR function is associated with progression of various human diseases, including fibrosis, arthritis, and cancer. By interacting with key components of the extracellular matrix and displaying distinct activation kinetics, the DDRs form a unique subfamily of receptor tyrosine kinases. DDR-facilitated cellular functions include cell migration, cell survival, proliferation, and differentiation, as well as remodeling of extracellular matrices. This review summarizes the current knowledge of DDR-ligand interactions, DDR-initiated signal pathways and the molecular mechanisms that regulate receptor function. Also discussed are the roles of DDRs in development and disease progression.


Assuntos
Regulação da Expressão Gênica , Receptores Proteína Tirosina Quinases/fisiologia , Receptores Mitogênicos/fisiologia , Sequência de Aminoácidos , Animais , Artrite/metabolismo , Aterosclerose/metabolismo , Diferenciação Celular , Movimento Celular , Proliferação de Células , Sobrevivência Celular , Colágeno/química , Receptor com Domínio Discoidina 1 , Receptores com Domínio Discoidina , Matriz Extracelular/metabolismo , Fibrose/metabolismo , Humanos , Nefropatias/metabolismo , Ligantes , Camundongos , Dados de Sequência Molecular , Neoplasias/metabolismo , Processamento de Proteína Pós-Traducional , Estrutura Terciária de Proteína , Transdução de Sinais , Distribuição Tecidual
11.
Cell Mol Life Sci ; 71(17): 3269-79, 2014 Sep.
Artigo em Inglês | MEDLINE | ID: mdl-24705941

RESUMO

The discoidin domain receptors (DDRs) are collagen-binding receptor tyrosine kinases that have been implicated in a number of fundamental biological processes ranging from growth and development to immunoregulation. In this review, we examine how recent proteomic technologies have enriched our understanding of DDR signaling mechanisms. We provide an overview on the use of large-scale proteomic profiling and chemical proteomics to reveal novel insights into DDR therapeutics, signaling networks, and receptor crosstalk. A perspective of how proteomics may be harnessed to answer outstanding fundamental questions including the dynamic regulation of receptor activation kinetics is presented. Collectively, these studies present an emerging molecular portrait of these unique receptors and their functional role in health and disease.


Assuntos
Processamento de Proteína Pós-Traducional , Receptores Proteína Tirosina Quinases/fisiologia , Receptores Mitogênicos/fisiologia , Transdução de Sinais/fisiologia , Animais , Antineoplásicos/farmacologia , Antineoplásicos/uso terapêutico , Fenômenos Fisiológicos Celulares/fisiologia , Receptores com Domínio Discoidina , Ativação Enzimática , Células Epiteliais/enzimologia , Transição Epitelial-Mesenquimal , Proteínas de Choque Térmico HSP90/antagonistas & inibidores , Proteínas de Choque Térmico HSP90/fisiologia , Humanos , Peptídeos e Proteínas de Sinalização Intracelular/fisiologia , Mesoderma/enzimologia , Terapia de Alvo Molecular , Proteínas de Neoplasias/antagonistas & inibidores , Proteínas de Neoplasias/fisiologia , Neoplasias/tratamento farmacológico , Neoplasias/enzimologia , Fosforilação , Mapeamento de Interação de Proteínas , Inibidores de Proteínas Quinases/farmacologia , Inibidores de Proteínas Quinases/uso terapêutico , Processamento de Proteína Pós-Traducional/efeitos dos fármacos , Proteômica , Receptores Proteína Tirosina Quinases/antagonistas & inibidores , Receptores Mitogênicos/antagonistas & inibidores , Transdução de Sinais/efeitos dos fármacos
12.
Blood ; 121(9): 1489-91, 2013 Feb 28.
Artigo em Inglês | MEDLINE | ID: mdl-23449615

RESUMO

The mechanisms that regulate 3-dimensional (3D) neutrophil chemotaxis are poorly understood. In this issue of Blood, Afonso et al demonstrate that the collagen receptor Discoidin domain receptor 2 (DDR2) promotes neutrophil chemotaxis in 3D by triggering matrix metalloproteinase (MMP) activity and the generation of chemotactic collagen peptides.


Assuntos
Quimiotaxia de Leucócito , Neutrófilos/fisiologia , Receptores Proteína Tirosina Quinases/fisiologia , Receptores Mitogênicos/fisiologia , Técnicas de Cultura de Tecidos , Receptores com Domínio Discoidina , Humanos
13.
Blood ; 121(9): 1644-50, 2013 Feb 28.
Artigo em Inglês | MEDLINE | ID: mdl-23233663

RESUMO

Neutrophils express a variety of collagen receptors at their surface, yet their functional significance remains unclear. Although integrins are essential for neutrophil adhesion and migration on 2-dimensional (2D) surfaces, neutrophils can compensate for the absence of integrins in 3-dimensional (3D) lattices. In contrast, we demonstrate that the inhibition of the tyrosine-kinase collagen receptor discoidin domain receptor 2 (DDR2) has no impact on human primary neutrophil migration on 2D surfaces but is an important regulator of neutrophil chemotaxis in 3D collagen matrices. In this context, we show that DDR2 activation specifically regulates the directional migration of neutrophils in chemoattractant gradients. We further demonstrate that DDR2 regulates directionality through its ability to increase secretion of metalloproteinases and local generation of collagen-derived chemotactic peptide gradients. Our findings highlight the importance of collagen-derived extracellular signaling during neutrophil chemotaxis in 3D matrices.


Assuntos
Quimiotaxia de Leucócito , Neutrófilos/fisiologia , Receptores Proteína Tirosina Quinases/fisiologia , Receptores Mitogênicos/fisiologia , Técnicas de Cultura de Tecidos , Ensaios de Migração de Leucócitos/métodos , Polaridade Celular/efeitos dos fármacos , Células Cultivadas , Quimiotaxia de Leucócito/efeitos dos fármacos , Quimiotaxia de Leucócito/fisiologia , Colágeno/química , Colágeno/farmacologia , Dipeptídeos/farmacologia , Receptores com Domínio Discoidina , Matriz Extracelular/química , Matriz Extracelular/efeitos dos fármacos , Matriz Extracelular/metabolismo , Humanos , Metaloproteinase 2 da Matriz/metabolismo , Neutrófilos/efeitos dos fármacos , Neutrófilos/metabolismo , Cultura Primária de Células , Inibidores de Proteases/farmacologia , Receptores Proteína Tirosina Quinases/metabolismo , Receptores Mitogênicos/metabolismo , Técnicas de Cultura de Tecidos/métodos , Alicerces Teciduais/química
14.
Biochem Biophys Res Commun ; 429(1-2): 39-44, 2012 Dec 07.
Artigo em Inglês | MEDLINE | ID: mdl-23131558

RESUMO

Discoidin domain receptor-2 (DDR2) is a cell surface tyrosine kinase receptor that can be activated by soluble collagen and has been implicated in diverse physiological functions including organism growth and wound repair. In the current studies, we used fibronectin and collagen-coated 2D surfaces and collagen matrices in combination with siRNA technology to investigate the role of DDR2 in a range of fibroblast motile activities. Silencing DDR2 with siRNA inhibited cell spreading and migration, and similar inhibition occurred regardless whether cells were interacting with fibronectin or collagen surfaces. Under the assay conditions used, DDR2 tyrosine kinase activation was not observed unless soluble collagen was added to the incubation medium. Finally silencing DDR2 also inhibited human fibroblast migration in 3D collagen matrices but had no effect on 3D collagen matrix remodeling and contraction. Taken together, our findings suggest that DDR2 is required for normal fibroblast spreading and migration independent of adhesion ligand and collagen activation of DDR2 tyrosine kinase.


Assuntos
Movimento Celular , Colágeno/metabolismo , Fibroblastos/fisiologia , Receptores Proteína Tirosina Quinases/fisiologia , Receptores Mitogênicos/fisiologia , Adesão Celular , Células Cultivadas , Receptores com Domínio Discoidina , Ativação Enzimática , Fibroblastos/enzimologia , Humanos , Ligantes , RNA Interferente Pequeno/genética , Receptores Proteína Tirosina Quinases/genética , Receptores Mitogênicos/genética , Solubilidade
15.
Biochem Biophys Res Commun ; 427(3): 611-7, 2012 Oct 26.
Artigo em Inglês | MEDLINE | ID: mdl-23022180

RESUMO

Discoidin domain receptor 2 (DDR2) is a receptor tyrosine kinase that is activated by fibrillar collagens. DDR2 regulates cell proliferation, cell adhesion, migration, and extracellular matrix remodeling. The decrement of endogenous DDR2 represses osteoblastic marker gene expression and osteogenic differentiation in murine preosteoblastic cells, but the functions of DDR2 in chondrogenic cellular proliferation remain unclear. To better understand the role of DDR2 signaling in cellular proliferation in endochondral ossification, we inhibited Ddr2 expression via the inhibitory effect of miRNA on Ddr2 mRNA (miDdr2) and analyzed the cellular proliferation and differentiation in the prechondrocyte ATDC5 cell lines. To investigate DDR2's molecular role in endochondral cellular proliferation in vivo, we also produced transgenic mice in which the expression of truncated, kinase dead (KD) DDR2 protein is induced, and evaluated the DDR2 function in cellular proliferation in chondrocytes. Although the miDdr2-transfected ATDC5 cell lines retained normal differentiation ability, DDR2 reduction finally promoted cellular proliferation in proportion to the decreasing ratio of Ddr2 expression, and it also promoted earlier differentiation to cartilage cells by insulin induction. The layer of hypertrophic chondrocytes in KD Ddr2 transgenic mice was not significantly thicker than that of normal littermates, but the layer of proliferative chondrocytes in KD-Ddr2 transgenic mice was significantly thicker than that of normal littermates. Taken together, our data demonstrated that DDR2 might play a local and essential role in the proliferation of chondrocytes.


Assuntos
Proliferação de Células , Condrócitos/fisiologia , Receptores Proteína Tirosina Quinases/fisiologia , Receptores Mitogênicos/fisiologia , Animais , Cartilagem/citologia , Cartilagem/fisiologia , Linhagem Celular , Condrócitos/citologia , Subunidade alfa 1 de Fator de Ligação ao Core/genética , Receptores com Domínio Discoidina , Expressão Gênica , Camundongos , Camundongos Transgênicos , Osteogênese/genética , Osteogênese/fisiologia , Receptores Proteína Tirosina Quinases/genética , Receptores Mitogênicos/genética , Transfecção
16.
Am J Physiol Cell Physiol ; 303(12): C1207-17, 2012 Dec 15.
Artigo em Inglês | MEDLINE | ID: mdl-23015544

RESUMO

As increase in collagen deposition is no longer taken as simply a consequence but, rather, an inducer of disease progression; therefore, the understanding of collagen signal transduction is fundamentally important. Cells contain at least two types of collagen receptors: integrins and discoidin domain receptors (DDRs). The integrin heterodimers α(1)ß(1), α(2)ß(1), α(10)ß(1), and α(11)ß(1) are recognized as the non-tyrosine kinase collagen receptors. DDR1 and 2, the tyrosine kinase receptors of collagen, are specifically expressed in epithelium and mesenchyme, respectively. While integrin ß(1) and DDR1 are both required for cell adhesion on collagen, their roles in epithelial cell differentiation during development and disease progression seem to counteract each other, with integrin ß(1) favoring epithelium mesenchyme transition (EMT) and DDR1 inducing epithelial cell differentiation. The in vitro evidence shows that the integrin ß(1) and DDR1 exert opposing actions in regulation of membrane stability of E-cadherin, which itself is a critical regulator of epithelial cell differentiation. Here, we review the functional roles of integrin ß(1) and DDR1 in regulation of epithelial cell differentiation during development and disease progression, and explore the underlining mechanisms regarding to the regulation of membrane stability of E-cadherin.


Assuntos
Diferenciação Celular/fisiologia , Células Epiteliais/fisiologia , Integrina beta1/fisiologia , Receptores Proteína Tirosina Quinases/fisiologia , Receptores de Colágeno/fisiologia , Receptores Mitogênicos/fisiologia , Animais , Caderinas/fisiologia , Colágeno/fisiologia , Receptores com Domínio Discoidina , Feminino , Fibrose , Humanos , Mesoderma/fisiologia , Camundongos , Neoplasias/patologia
17.
Biochim Biophys Acta ; 1820(11): 1797-808, 2012 Nov.
Artigo em Inglês | MEDLINE | ID: mdl-22846227

RESUMO

BACKGROUND: Complex carbohydrates play important functions for circulation of proteins and cells. They provide protective shields and refraction from non-specific interactions with negative charges from sialic acids to enhance circulatory half-life. For recombinant protein therapeutics carbohydrates are especially important to enhance size and reduce glomerular filtration loss. Carbohydrates are, however, also ligands for a large number of carbohydrate-binding lectins exposed to the circulatory system that serve as scavenger receptors for the innate immune system, or have more specific roles in targeting of glycoproteins and cells. SCOPE OF REVIEW: Here we provide an overview of the common lectin receptors that play roles for circulating glycoproteins and cells, and present a discussion of ways to engineer glycosylation of recombinant biologics and cells to improve therapeutic effects. MAJOR CONCLUSIONS: While the pharmaceutical industry has learned how to exploit carbohydrates to improve pharmacokinetic properties of recombinant therapeutics, our understanding of how to improve cell-based therapies by manipulation of complex carbohydrates is still at its infancy. Progress with the latter has recently been achieved with cold-stored platelets, where exposure of uncapped glycans lead to rapid clearance from circulation by several lectin-mediated pathways. GENERAL SIGNIFICANCE: Understanding lectin-mediated clearance pathways is essential for progress in development of biological pharmaceuticals.


Assuntos
Metabolismo dos Carboidratos , Transfusão de Plaquetas , Receptores Mitogênicos/fisiologia , Animais , Endocitose , Glicosilação , Humanos , Receptores de Hialuronatos/fisiologia , Lectinas Tipo C/fisiologia , Antígeno de Macrófago 1/fisiologia , Receptor de Manose , Lectinas de Ligação a Manose/fisiologia , Taxa de Depuração Metabólica , Receptores de Superfície Celular/fisiologia , Proteínas Recombinantes/uso terapêutico
18.
Am J Pathol ; 179(6): 2894-904, 2011 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-22019896

RESUMO

Hepatic stellate cells (HSCs) interact with fibrillar collagen through the discoidin domain receptor 2 (DDR2) in acute hepatic injury, generating increased fibrosis. However, the contribution of DDR2 signaling to chronic liver fibrosis in vivo is unclear, despite its relevance to chronic human liver disease. We administered carbon tetrachloride (CCl(4)) to DDR2(+/+) and DDR2(-/-) mice twice weekly, and liver tissues and isolated HSCs were analyzed. In contrast to changes seen in acute injury, after chronic CCl(4) administration, DDR2(-/-) livers had increased collagen deposition, gelatinolytic activity, and HSC density. Increased basal gene expression of osteopontin, transforming growth factor-ß1, monocyte chemoattractant protein-1, and IL-10 and reduced basal gene expression of matrix metalloproteinase-2, matrix metalloproteinase-13, and collagen type I in quiescent DDR2(-/-) HSCs were amplified further after chronic CCl(4). In concordance, DDR2(-/-) HSCs isolated from chronically injured livers had enhanced in vitro migration and proliferation, but less extracellular matrix degradative activity. Macrophages from chronic CCl(4)-treated DDR2(-/-) livers showed stronger chemoattractive activity toward DDR2(-/-) HSCs than DDR2(+/+) macrophages, increased extracellular matrix degradation, and higher cytokine mRNA expression. In conclusion, loss of DDR2 promotes chronic liver fibrosis after CCl(4) injury. The fibrogenic sinusoidal milieu generated in chronic DDR2(-/-) livers recruits more HSCs to injured regions, which enhances fibrosis. Together, these findings suggest that DDR2 normally orchestrates gene programs and paracrine interactions between HSCs and macrophages that together attenuate chronic hepatic fibrosis.


Assuntos
Comunicação Celular/fisiologia , Células Estreladas do Fígado/fisiologia , Cirrose Hepática/patologia , Macrófagos/fisiologia , Receptores Proteína Tirosina Quinases/deficiência , Receptores Mitogênicos/deficiência , Lesão Pulmonar Aguda/induzido quimicamente , Lesão Pulmonar Aguda/patologia , Animais , Tetracloreto de Carbono/toxicidade , Movimento Celular/fisiologia , Proliferação de Células , Células Cultivadas , Colágeno Tipo I/metabolismo , Colagenases/metabolismo , Receptores com Domínio Discoidina , Gelatinases/metabolismo , Cirrose Hepática/fisiopatologia , Masculino , Camundongos , Camundongos Knockout , Receptores Proteína Tirosina Quinases/fisiologia , Receptores Mitogênicos/fisiologia , Transdução de Sinais/fisiologia
19.
Oncol Rep ; 24(2): 311-9, 2010 Aug.
Artigo em Inglês | MEDLINE | ID: mdl-20596615

RESUMO

Discoidin domain receptors (DDRs) are a novel class of receptor tyrosine kinases that bind to several collagens and facilitate cell adhesion. DDR1 is involved in cancer invasion. However, very limited data are available on the aspect of clinical significance of DDR1 expression in cancer. The aim of this study was to investigate prognostic impact of DDR1 expression in non-small cell lung carcinoma (NSCLC). Tumor tissues from 171 NSCLC, including 86 squamous cell carcinomas, 69 adenocarcinomas, and 16 pure bronchioloalveolar carcinomas (BAC), were analyzed for expression of DDR1 using immunohistochemical staining. In addition, two lung adenocarcinoma cell lines (A549, H358) were transfected with two isoforms of DDR1, DDR1a and DDR1b, and then migration and invasion assays were carried out. DDR1 was expressed in 6 of 16 BAC (38%) and 95 of 155 invasive NSCLC (61%, p=0.065). DDR1 up-regulation tend to be more frequently observed in invasive adenocarcinoma (64%) compared to DDR1 expression in BAC (38%) (p=0.056). In invasive NSCLC, DDR1 expression was significantly correlated with lymph node metastasis (p=0.001). Overexpression of DDR1 in lung cancer cells resulted in a significant increase of cell motility and invasiveness (p<0.001) and the interaction of DDR1 with collagen facilitates the invasiveness of NSCLC cells. DDR1 overexpression produced an activation of MMP-9 in H358 cells. In conclusion, these findings indicate that up-regulation of DDR1 may contribute to the progression and poor prognosis of NSCLC and this effect may be associated with increased invasiveness.


Assuntos
Carcinoma Pulmonar de Células não Pequenas/diagnóstico , Neoplasias Pulmonares/diagnóstico , Receptores Proteína Tirosina Quinases/fisiologia , Receptores Mitogênicos/fisiologia , Idoso , Biomarcadores Tumorais/genética , Biomarcadores Tumorais/metabolismo , Biomarcadores Tumorais/fisiologia , Carcinoma Pulmonar de Células não Pequenas/metabolismo , Carcinoma Pulmonar de Células não Pequenas/mortalidade , Carcinoma Pulmonar de Células não Pequenas/patologia , Adesão Celular/genética , Movimento Celular/genética , Receptores com Domínio Discoidina , Feminino , Regulação Neoplásica da Expressão Gênica , Humanos , Neoplasias Pulmonares/metabolismo , Neoplasias Pulmonares/mortalidade , Neoplasias Pulmonares/patologia , Metástase Linfática , Masculino , Pessoa de Meia-Idade , Invasividade Neoplásica , Neoplasias Pleurais/metabolismo , Neoplasias Pleurais/secundário , Prognóstico , Receptores Proteína Tirosina Quinases/genética , Receptores Proteína Tirosina Quinases/metabolismo , Receptores Mitogênicos/genética , Receptores Mitogênicos/metabolismo , Análise de Sobrevida , Células Tumorais Cultivadas
20.
Mol Immunol ; 47(9): 1866-9, 2010 May.
Artigo em Inglês | MEDLINE | ID: mdl-20353877

RESUMO

T cell migration through extracellular matrix of the tissue is an important process in the development of inflammation. However, the mechanisms regulating this process are complex and still not well defined. In this study, we show that activation of human peripheral blood T cells with anti-CD3 mAb increases the mRNA and protein levels of the discoidin domain receptor 1 (DDR1), which is known to bind to collagens. Furthermore, our findings indicate that DDR1 is involved in the migration of activated T cells in three-dimensional (3D) collagen. Indeed, the use of a DDR1 blocking molecule (DDR1:Fc) reduced the capacity of anti-CD3-activated human T cells to migrate in 3D collagen, whereas a control immunoglobulin had no effect. As a control, the DDR1:Fc molecule did not interfere with the capacity of human T cells to migrate through fibronectin. Together these results suggest that DDR1 can represent an additional receptor regulating T cell movement in the tissues and therefore can contribute to the development of inflammatory diseases.


Assuntos
Movimento Celular/fisiologia , Colágeno/fisiologia , Receptores Proteína Tirosina Quinases/fisiologia , Receptores Mitogênicos/fisiologia , Linfócitos T/fisiologia , Adulto , Western Blotting , Técnicas de Cultura de Células , Movimento Celular/efeitos dos fármacos , Células Cultivadas , Receptores com Domínio Discoidina , Expressão Gênica , Humanos , Células Jurkat , Muromonab-CD3/farmacologia , Receptores Proteína Tirosina Quinases/genética , Receptores Proteína Tirosina Quinases/metabolismo , Receptores Mitogênicos/genética , Receptores Mitogênicos/metabolismo , Reação em Cadeia da Polimerase Via Transcriptase Reversa , Linfócitos T/citologia , Linfócitos T/metabolismo
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